FastQCFastQC Report
Mon 16 Jan 2017
ERR1146434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4992949
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC179630.35976734390837956No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA141780.2839604410139178No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT132680.2657347391291199No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC125210.250773640988522No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC121040.2424218633116421No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA116850.23403002914710327No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC101460.20320656189358233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90690.18163614328926653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84250.1687379542631018No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG75130.15047219589064498No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC72490.14518473951967065No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT69100.13839516486148767No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT68920.13803465647255758No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG66290.13276722834541269No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC65170.1305240650365145No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG64520.12922222918760037No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA61840.12385465984130822No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT59680.11952855917414738No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT59520.1192081072728762No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC59420.11900782483458172No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA58730.11762587601034981No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT58160.11648426611207123No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA56930.1140207921210491No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT56370.1128992104666No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC55260.11067607540153125No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT55140.11043573647557786No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA55040.11023545403728338No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG54070.10829271438582688No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC53090.10632994649054098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96650.045.3955731
GTATCAA167750.038.405351
GGGCACC51700.037.57224794
CAACGCA191650.032.174425
ATCAACG194050.031.7640383
GTACTAG44500.031.5969261
CTGTGCG65400.031.3359059
TAGTACT67050.030.9931554
TATCAAC214400.029.9558242
AACGCAG221150.028.8799516
GTGGTAT28150.028.2320231
TAGGCAT58250.028.1588695
ACTGTGC77450.028.0336848
GTACTGT77100.027.5516746
TACTGTG86700.027.1028427
ACGCAGA232300.026.4619887
TCAACGC235150.026.3919354
CGCAGAG234400.026.2046018
CTAGTAC81500.026.1328773
TATTCTC32250.025.6488955
Produced by FastQC (version 0.11.5)