##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146434_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4992949
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88284759167378	31.0	31.0	34.0	30.0	34.0
2	31.881040242950608	31.0	31.0	34.0	30.0	34.0
3	32.04442845300443	33.0	31.0	34.0	30.0	34.0
4	35.78064366369454	37.0	35.0	37.0	35.0	37.0
5	35.67973916817496	37.0	35.0	37.0	33.0	37.0
6	35.71175611847828	37.0	35.0	37.0	33.0	37.0
7	35.645932293720605	37.0	35.0	37.0	33.0	37.0
8	35.62831545044822	37.0	35.0	37.0	33.0	37.0
9	37.23789407822912	39.0	37.0	39.0	33.0	39.0
10-11	37.179205715900565	39.0	37.0	39.0	33.0	39.0
12-13	37.11389461418493	39.0	37.0	39.0	33.0	39.0
14-15	38.3284718109478	40.0	38.0	41.0	33.0	41.0
16-17	38.33131662270134	40.0	38.0	41.0	33.0	41.0
18-19	38.39043839622636	40.0	38.0	41.0	33.5	41.0
20-21	38.404495419440494	40.0	38.0	41.0	34.0	41.0
22-23	38.37376698620395	40.0	38.0	41.0	34.0	41.0
24-25	38.30353164031918	40.0	38.0	41.0	33.0	41.0
26-27	38.16448936289956	40.0	37.0	41.0	33.0	41.0
28-29	38.10391313830765	40.0	37.0	41.0	33.0	41.0
30-31	37.98239427240294	40.0	37.0	41.0	33.0	41.0
32-33	37.86377569648718	40.0	37.0	41.0	33.0	41.0
34-35	37.77062233161204	40.0	37.0	41.0	33.0	41.0
36-37	37.69337479713893	40.0	36.0	41.0	32.5	41.0
38-39	37.5829801185632	40.0	36.0	41.0	32.0	41.0
40-41	37.460802824142604	40.0	36.0	41.0	32.0	41.0
42-43	37.33826912712307	40.0	36.0	41.0	31.0	41.0
44-45	37.148225828062735	39.0	35.5	41.0	31.0	41.0
46-47	36.92326268503844	39.0	35.0	41.0	30.5	41.0
48-49	36.67245609758882	39.0	35.0	41.0	30.0	41.0
50-51	36.47797534082563	39.0	35.0	41.0	30.0	41.0
52-53	36.293213890228	39.0	35.0	41.0	30.0	41.0
54-55	36.09505965312283	38.0	35.0	40.0	29.0	41.0
56-57	35.841348469611845	38.0	35.0	40.0	29.0	41.0
58-59	35.570151427543124	37.0	34.0	40.0	28.0	41.0
60-61	35.312543849336336	37.0	34.0	40.0	28.0	41.0
62-63	35.03611943562812	36.5	34.0	40.0	28.0	41.0
64-65	34.7629370938898	36.0	34.0	39.0	28.0	41.0
66-67	34.53791837248888	35.5	34.0	39.0	28.0	41.0
68-69	34.34134075873797	35.0	33.0	39.0	27.5	41.0
70-71	34.125226494402405	35.0	33.0	38.0	27.0	40.5
72-73	33.912017827540396	35.0	33.0	37.0	27.0	40.0
74-75	33.72172487642073	35.0	33.0	37.0	27.0	39.5
76-77	33.553478014696324	35.0	33.0	36.5	27.0	39.0
78-79	33.42996463612987	35.0	33.0	36.0	27.0	39.0
80-81	33.272238610889076	35.0	33.0	36.0	27.0	38.0
82-83	33.07488320028905	35.0	33.0	35.5	27.0	37.0
84-85	32.92479053961897	35.0	33.0	35.0	27.0	37.0
86-87	32.78540397668793	35.0	33.0	35.0	27.0	36.5
88-89	32.670109989106635	35.0	33.0	35.0	27.0	36.0
90-91	32.57126339564053	35.0	33.0	35.0	27.0	36.0
92-93	32.494680899003775	35.0	33.0	35.0	27.0	36.0
94-95	32.44640101471094	35.0	33.0	35.0	27.0	35.5
96-97	32.393481587735025	35.0	33.0	35.0	27.0	35.0
98-99	32.35464261701852	35.0	33.0	35.0	26.0	35.0
100	31.856861345869945	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.44727940844010305
1101	2	0.567727231870613
1101	3	0.47114402576443837
1101	4	0.3136946929765898
1101	5	0.3567958764664638
1101	6	0.31971034103668927
1101	7	0.3193446037464227
1101	8	0.3629775466340064
1101	9	0.4263928753673767
1101	10-11	0.481725603535196
1101	12-13	0.49679918850631566
1101	14-15	0.6321405718361675
1101	16-17	0.6143947092539861
1101	18-19	0.6028781405721091
1101	20-21	0.586353592234687
1101	22-23	0.5999800434084648
1101	24-25	0.6575851350206605
1101	26-27	0.6369027778519083
1101	28-29	0.6838072207211923
1101	30-31	0.7344562225300635
1101	32-33	0.7424389632837674
1101	34-35	0.74413122157938
1101	36-37	0.730130451238324
1101	38-39	0.7497423070843396
1101	40-41	0.717081574792843
1101	42-43	0.7072033308732273
1101	44-45	0.6876395261160084
1101	46-47	0.6819422013927152
1101	48-49	0.7409923247072001
1101	50-51	0.7328388696710348
1101	52-53	0.7174466065132776
1101	54-55	0.6720766106987099
1101	56-57	0.6812863680330068
1101	58-59	0.6345207501952999
1101	60-61	0.7203416254405113
1101	62-63	0.6565440254515522
1101	64-65	0.6403643570909949
1101	66-67	0.5830926350891019
1101	68-69	0.6039094157384639
1101	70-71	0.6440676814111512
1101	72-73	0.6803041743346157
1101	74-75	0.5355574565536685
1101	76-77	0.5551264778598224
1101	78-79	0.529903098349024
1101	80-81	0.5455876959475745
1101	82-83	0.495961715412939
1101	84-85	0.5185617447537183
1101	86-87	0.5270160343293995
1101	88-89	0.5120909645743197
1101	90-91	0.49808211521128243
1101	92-93	0.48379250953782815
1101	94-95	0.5031765540835309
1101	96-97	0.5083294572263028
1101	98-99	0.5147556104170974
1101	100	0.5568457654822687
1102	1	0.3928769909358891
1102	2	0.45140004859173644
1102	3	0.3668176043585163
1102	4	0.2713071592784857
1102	5	0.2873678999529119
1102	6	0.32174116039498557
1102	7	0.3179643359607738
1102	8	0.3390986382622856
1102	9	0.4460112818808142
1102	10-11	0.4020921114534275
1102	12-13	0.4419370255992163
1102	14-15	0.5254134306883174
1102	16-17	0.496283787089439
1102	18-19	0.495122846212233
1102	20-21	0.4667717924754058
1102	22-23	0.5410325599787456
1102	24-25	0.5829336350730543
1102	26-27	0.5568447562710688
1102	28-29	0.5435995905980278
1102	30-31	0.5940670793228691
1102	32-33	0.6013199348983846
1102	34-35	0.5565330694752504
1102	36-37	0.5694636260052306
1102	38-39	0.5333830150826557
1102	40-41	0.5387079885846546
1102	42-43	0.6300345874494226
1102	44-45	0.5508745529495513
1102	46-47	0.5453538502125355
1102	48-49	0.5498298695988026
1102	50-51	0.477826272604311
1102	52-53	0.5547792422634288
1102	54-55	0.6155355117914141
1102	56-57	0.6156557953496673
1102	58-59	0.6153019463682128
1102	60-61	0.5884404132361851
1102	62-63	0.536011734219656
1102	64-65	0.5812203048890794
1102	66-67	0.5298713643345963
1102	68-69	0.48751913729858387
1102	70-71	0.452439193616037
1102	72-73	0.48200830215611035
1102	74-75	0.40680084670193395
1102	76-77	0.4573107122795008
1102	78-79	0.42694534838062026
1102	80-81	0.38161740550690126
1102	82-83	0.41920151204500655
1102	84-85	0.4516799670150604
1102	86-87	0.4753236584818765
1102	88-89	0.4221989641034227
1102	90-91	0.4452370321242469
1102	92-93	0.42644963520829293
1102	94-95	0.4476631014926653
1102	96-97	0.44817282328902763
1102	98-99	0.4098446227936563
1102	100	0.4567312906733143
1103	1	0.2863370995481489
1103	2	-0.15943664514601252
1103	3	0.20741372287943705
1103	4	0.18738043802163418
1103	5	0.24341560440599608
1103	6	0.29660906067934434
1103	7	0.32309357200759337
1103	8	0.33298502483503256
1103	9	0.4312487811816652
1103	10-11	0.4122958539764596
1103	12-13	0.4668532002615322
1103	14-15	0.5384596787642622
1103	16-17	0.5400543032172891
1103	18-19	0.5454991293463607
1103	20-21	0.5465604490815323
1103	22-23	0.4802991335235163
1103	24-25	0.4982394709358786
1103	26-27	0.5568750045630253
1103	28-29	0.5558204096599724
1103	30-31	0.5589933070216802
1103	32-33	0.5673615573907824
1103	34-35	0.556963804801569
1103	36-37	0.5201940915336394
1103	38-39	0.5855985365679501
1103	40-41	0.6354821056812554
1103	42-43	0.6266978918683321
1103	44-45	0.6611039810651249
1103	46-47	0.6198146028693898
1103	48-49	0.6072215795724958
1103	50-51	0.5735915222640173
1103	52-53	0.5419420462249747
1103	54-55	0.5178361708952011
1103	56-57	0.5268800165807974
1103	58-59	0.47075021323939836
1103	60-61	0.49984636121958204
1103	62-63	0.49355572845552587
1103	64-65	0.5338737840728953
1103	66-67	0.5373322468677486
1103	68-69	0.48549723297075786
1103	70-71	0.42926799253202574
1103	72-73	0.46160408245091133
1103	74-75	0.4420412303872112
1103	76-77	0.41218253413289574
1103	78-79	0.38164825906684285
1103	80-81	0.3721984595708676
1103	82-83	0.4012744834950155
1103	84-85	0.3783235842374779
1103	86-87	0.4242150787706649
1103	88-89	0.40347622816349826
1103	90-91	0.38642741810404146
1103	92-93	0.42754311184022953
1103	94-95	0.4169125088245167
1103	96-97	0.43775351560238107
1103	98-99	0.45078249961355255
1103	100	0.4912729791923134
1104	1	0.31702602910709743
1104	2	0.4200905695218964
1104	3	0.3485096939185581
1104	4	0.24380840143061278
1104	5	0.24709330749742975
1104	6	0.2533394381507179
1104	7	0.2682576267486567
1104	8	0.2692275639482915
1104	9	0.3295999416182056
1104	10-11	0.35312766901866155
1104	12-13	0.3600333523066652
1104	14-15	0.42320625676436663
1104	16-17	0.41666336027168427
1104	18-19	0.4006353899942212
1104	20-21	0.4330635039844637
1104	22-23	0.4114484929017266
1104	24-25	0.40211022806070673
1104	26-27	0.5001889624233016
1104	28-29	0.43863279002874833
1104	30-31	0.46282793551682744
1104	32-33	0.5186150465208783
1104	34-35	0.47486667217743417
1104	36-37	0.43265122595703076
1104	38-39	0.427239489357504
1104	40-41	0.3875891704314185
1104	42-43	0.4419942546416067
1104	44-45	0.4513303732444385
1104	46-47	0.4494591074074492
1104	48-49	0.48019921372900143
1104	50-51	0.5037383378038811
1104	52-53	0.49564064376420447
1104	54-55	0.5843727505687681
1104	56-57	0.5160391034130996
1104	58-59	0.468183525326225
1104	60-61	0.4605117514552646
1104	62-63	0.4671394280654866
1104	64-65	0.47261335154654915
1104	66-67	0.46956507206233766
1104	68-69	0.4644766255011703
1104	70-71	0.4746989069356786
1104	72-73	0.43091190575874094
1104	74-75	0.4006156889978172
1104	76-77	0.4062648730914944
1104	78-79	0.3914521233331172
1104	80-81	0.4347910199579701
1104	82-83	0.3731846619109689
1104	84-85	0.3341892501308479
1104	86-87	0.3240488973282467
1104	88-89	0.3390513104971262
1104	90-91	0.3285688703368592
1104	92-93	0.3651109598344746
1104	94-95	0.41502655675685673
1104	96-97	0.4180132869440385
1104	98-99	0.3872662836353058
1104	100	0.3799515791761259
1105	1	0.09757541198742103
1105	2	-0.42007790116538857
1105	3	0.0010646740746054206
1105	4	0.06177750257081982
1105	5	0.11757840046995227
1105	6	0.19583895226675452
1105	7	0.1887149947656397
1105	8	0.1669326762002541
1105	9	0.21630048777059585
1105	10-11	0.30823845089801694
1105	12-13	0.24799998572085258
1105	14-15	0.32052450155257617
1105	16-17	0.2988673880548305
1105	18-19	0.28847947715602373
1105	20-21	0.33258357428508845
1105	22-23	0.3423936628791253
1105	24-25	0.3122928387777293
1105	26-27	0.3303615886617308
1105	28-29	0.30735276502085185
1105	30-31	0.283640373487664
1105	32-33	0.34587147013535
1105	34-35	0.39737747116690514
1105	36-37	0.3825017259134853
1105	38-39	0.4414070978863762
1105	40-41	0.4392004049960363
1105	42-43	0.4648398733795531
1105	44-45	0.45060825928765524
1105	46-47	0.41748002104939985
1105	48-49	0.42543682341915456
1105	50-51	0.4479950986473611
1105	52-53	0.37481592764677885
1105	54-55	0.345788024704099
1105	56-57	0.3586952260617764
1105	58-59	0.32310022915662273
1105	60-61	0.3755643828361386
1105	62-63	0.40157614977749034
1105	64-65	0.39350681386022757
1105	66-67	0.38341706434786715
1105	68-69	0.354610180419094
1105	70-71	0.3499054076195449
1105	72-73	0.3525249786851532
1105	74-75	0.3322508662843049
1105	76-77	0.30059204174686016
1105	78-79	0.26877674711243316
1105	80-81	0.2828151859310921
1105	82-83	0.26860045761550566
1105	84-85	0.3157253487554854
1105	86-87	0.2819935486934
1105	88-89	0.26857237171877557
1105	90-91	0.2471698491208727
1105	92-93	0.2599413930521095
1105	94-95	0.26056489367349656
1105	96-97	0.25803968025494584
1105	98-99	0.25188703272187496
1105	100	0.28278915159501494
1106	1	0.2322137125495729
1106	2	0.2450651472127845
1106	3	0.21405655146804037
1106	4	0.16940266176862906
1106	5	0.16906366561994446
1106	6	0.18770067670318014
1106	7	0.20251646173497306
1106	8	0.19859573573916833
1106	9	0.21236857068447534
1106	10-11	0.20588738070605928
1106	12-13	0.19854840828923415
1106	14-15	0.28071358630899823
1106	16-17	0.2117653466562217
1106	18-19	0.2797095971730599
1106	20-21	0.26685959684237304
1106	22-23	0.2739167688346953
1106	24-25	0.26936564646350547
1106	26-27	0.2934986173035483
1106	28-29	0.29935473370225907
1106	30-31	0.28086621686957614
1106	32-33	0.2903821403327882
1106	34-35	0.27110498913576464
1106	36-37	0.3238999026531033
1106	38-39	0.30485211762371733
1106	40-41	0.3888250831321116
1106	42-43	0.3206312039749619
1106	44-45	0.30777030176971465
1106	46-47	0.3245111246353787
1106	48-49	0.27708208277194757
1106	50-51	0.27179866389310803
1106	52-53	0.3306216677098419
1106	54-55	0.286860635341327
1106	56-57	0.28528856013111437
1106	58-59	0.2800430993926213
1106	60-61	0.30857630285360216
1106	62-63	0.2803235781527391
1106	64-65	0.2655166050149944
1106	66-67	0.2557368444793724
1106	68-69	0.3125450421081766
1106	70-71	0.30154148941434045
1106	72-73	0.25191125496849764
1106	74-75	0.24934385758170663
1106	76-77	0.2615375901459558
1106	78-79	0.22722288932157397
1106	80-81	0.2535385670998167
1106	82-83	0.2512574538689307
1106	84-85	0.23188974290744113
1106	86-87	0.200661007362946
1106	88-89	0.18961228926841756
1106	90-91	0.16277250622938055
1106	92-93	0.1733056881987025
1106	94-95	0.18416849706942884
1106	96-97	0.19392715437331987
1106	98-99	0.19677087739502497
1106	100	0.17585337834757908
1107	1	0.044505464509967396
1107	2	-0.5126401308911959
1107	3	-0.028054589196898405
1107	4	0.0580416130312571
1107	5	0.12848512528146472
1107	6	0.17777108846348
1107	7	0.1811281942041134
1107	8	0.1422327619635979
1107	9	0.14970063822197943
1107	10-11	0.2039180449635083
1107	12-13	0.1856604713423735
1107	14-15	0.29030255575129615
1107	16-17	0.32067929858577315
1107	18-19	0.27392394976585166
1107	20-21	0.20190662668846215
1107	22-23	0.22525233764592656
1107	24-25	0.24701301721390934
1107	26-27	0.2518194361870627
1107	28-29	0.21778176077711464
1107	30-31	0.25565088076677256
1107	32-33	0.31032518914874174
1107	34-35	0.340127606081154
1107	36-37	0.29653050416524707
1107	38-39	0.3535008782561917
1107	40-41	0.32489657559639085
1107	42-43	0.34028007295670903
1107	44-45	0.30680244571613
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2216	72-73	-0.7112749053358911
2216	74-75	-0.8052463633389522
2216	76-77	-0.689801759088148
2216	78-79	-0.5787583873586968
2216	80-81	-0.49911740322531983
2216	82-83	-0.4496662038551946
2216	84-85	-0.5842736329005405
2216	86-87	-0.5079247977597348
2216	88-89	-0.5198120520123979
2216	90-91	-0.5578358327574477
2216	92-93	-0.6044253317002557
2216	94-95	-0.5857445475459215
2216	96-97	-0.609756081317208
2216	98-99	-0.5515445780224795
2216	100	-0.5722661324556064
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	13.0
13	56.0
14	268.0
15	819.0
16	2061.0
17	4027.0
18	6995.0
19	11101.0
20	16242.0
21	23492.0
22	32137.0
23	38837.0
24	41323.0
25	43294.0
26	48230.0
27	58364.0
28	73624.0
29	92084.0
30	113751.0
31	141436.0
32	175878.0
33	223689.0
34	305219.0
35	423166.0
36	633381.0
37	1097906.0
38	998529.0
39	386178.0
40	846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.17639916078997	21.371912904516822	13.869279856996176	24.582408077697036
2	14.732729785486812	20.21038716526128	40.34598513830192	24.710897910949985
3	17.946144522039944	26.367096588637658	29.94541788207282	25.741341007249574
4	12.23745283852339	17.36495936094584	39.01683759623866	31.380750204292106
5	12.689921100337145	38.58720873290062	35.17098732433944	13.551882842422794
6	29.6184279070345	38.896191409125144	17.794253456223966	13.691127227616384
7	25.949178086993097	32.46746145796301	23.79108438440393	17.792276070639957
8	26.055122934362036	35.02641424937447	19.805489701577166	19.11297311468633
9	26.05021601462382	14.715131278128416	21.488122550420602	37.746530156827156
10-11	25.45321412255563	26.041233347266314	27.48441852700679	21.02113400317127
12-13	25.881638286311354	23.84508634075774	27.902007410850782	22.371267962080125
14-15	23.194375062343738	24.829533686851548	25.70282677033187	26.27326448047284
16-17	21.774937199592532	27.39144272094107	27.002132269615338	23.831487809851062
18-19	21.255544568951134	27.276044678205203	29.495414433434032	21.97299631940963
20-21	22.78338051489236	26.420921994657864	28.76723193408942	22.02846555636036
22-23	23.091562270487465	26.09061953538601	28.516985755226553	22.30083243889997
24-25	22.624637526303346	26.18953510135344	29.212228005155673	21.973599367187543
26-27	22.546799316595237	26.44282934218236	28.262718479677762	22.747652861544644
28-29	21.733198156039645	26.912972674064967	28.87112405914821	22.482705110747176
30-31	22.59445269719358	26.448157191271132	28.669840208662258	22.28754990287303
32-33	23.013784038250744	26.747879860178823	27.906764118760275	22.331571982810157
34-35	22.861642365454408	26.609214055123783	28.640928321296137	21.88821525812567
36-37	22.695705483873358	26.16050153927068	28.391487675920583	22.75230530093538
38-39	22.773615352369912	26.143046924773316	29.016258728058308	22.067078994798464
40-41	22.382345206715172	25.556531601765936	29.39607841723492	22.665044774283974
42-43	22.55505118596397	25.785594666369178	28.543228648798102	23.116125498868747
44-45	22.712929773566685	26.600682282154292	28.252611833207187	22.433776111071836
46-47	22.771292076085697	26.395633121828403	27.600261889316315	23.23281291276959
48-49	23.5454391509425	26.173998825738305	27.601128356737618	22.67943366658158
50-51	23.034022578640396	26.277546596209977	27.268834510426604	23.419596314723023
52-53	22.424883570811556	26.807864450448022	27.213406345628606	23.553845633111813
54-55	22.543998195849653	26.71939053045385	27.031071122138258	23.705540151558242
56-57	23.457446110717612	26.234053626261293	26.88664436653675	23.42185589648435
58-59	22.428348828545907	26.551300436215936	27.742782809326773	23.277567925911388
60-61	22.612234556842424	27.0858746261602	27.017668421308255	23.28422239568912
62-63	23.006503771618735	26.381302913368433	26.657953045384602	23.95424026962823
64-65	22.14864401779389	26.56597333559786	27.071706520535262	24.21367612607299
66-67	22.81886916930255	26.190213438991666	27.066899742016194	23.924017649689592
68-69	22.337100057411657	26.098389739726812	27.394873728904624	24.16963647395691
70-71	22.32944422012645	25.83896932291216	27.494335970672022	24.337250486289363
72-73	22.909627156215695	25.863132189012944	27.737014738183785	23.49022591658757
74-75	22.980882111276486	26.324141790966305	27.250339303995453	23.444636793761752
76-77	22.671517917193594	26.287142971021083	26.82360526213537	24.217733849649957
78-79	22.388170754410226	26.32659267980509	27.070667256702922	24.214569309081764
80-81	22.056991919657033	26.69799914697087	27.591726919318045	23.65328201405405
82-83	22.834307862582364	25.72166371109978	28.439556821190674	23.004471605127183
84-85	23.108158235870025	26.259051309044818	27.077164262331333	23.555626192753827
86-87	21.82456789037887	26.83507256124687	27.494606453907938	23.845753094466325
88-89	22.683734227251758	25.665766859467233	27.355514245122382	24.294984668158623
90-91	22.34515749215521	25.655426566937305	27.66560333594684	24.333812604960634
92-93	22.61880046257391	26.01009774763122	27.271019255985905	24.100082533808965
94-95	21.50378816138854	25.88782075194415	27.986398570357757	24.62199251630955
96-97	21.836539511146764	25.814733696463136	27.561972918488074	24.786753873902022
98-99	21.240762165820907	26.48970763294435	27.425115172184704	24.844415029050037
100	21.99362717958196	25.749946980026404	26.736960393979498	25.51946544641214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	168.0
1	120.5
2	107.0
3	159.0
4	113.5
5	85.0
6	183.0
7	461.0
8	792.5
9	925.0
10	754.0
11	557.5
12	593.0
13	687.0
14	856.0
15	1205.5
16	1573.0
17	2030.0
18	2753.5
19	3615.5
20	4671.0
21	5862.0
22	7214.5
23	8562.5
24	9816.5
25	11572.5
26	14140.0
27	16984.0
28	21512.0
29	30919.0
30	43463.5
31	55081.0
32	71916.0
33	88487.0
34	100080.5
35	113281.0
36	126607.0
37	150354.5
38	161142.5
39	150286.0
40	140201.0
41	131164.0
42	130112.0
43	137635.5
44	149164.0
45	177110.0
46	203590.5
47	219793.0
48	231303.0
49	225494.0
50	214115.0
51	217644.0
52	219727.5
53	212056.0
54	204841.5
55	179676.0
56	157093.5
57	131885.5
58	105428.0
59	100704.5
60	84529.0
61	59856.5
62	44605.5
63	36107.0
64	26159.5
65	13796.5
66	8307.0
67	5870.0
68	4183.0
69	2939.5
70	2178.0
71	1580.0
72	1168.5
73	1010.0
74	810.0
75	583.5
76	407.5
77	242.5
78	125.0
79	65.5
80	33.5
81	20.5
82	16.0
83	3.0
84	1.0
85	0.5
86	1.5
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012717934831699664
2	0.004987032713532623
3	0.0021029656020920703
4	0.026477338342530635
5	0.00304429306207614
6	0.0
7	4.806778519067589E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009353189868352351
16-17	0.0029441518429288984
18-19	0.0
20-21	5.007060957362072E-5
22-23	6.008473148834487E-4
24-25	1.0014121914724145E-5
26-27	1.5021182872086217E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.5063548616258644E-4
36-37	0.0
38-39	0.0
40-41	3.2045190127117263E-4
42-43	5.507767053098279E-4
44-45	0.0
46-47	0.0
48-49	4.90691973821483E-4
50-51	0.0
52-53	0.0
54-55	3.404801451006209E-4
56-57	7.0098853403069E-5
58-59	1.802541944650346E-4
60-61	3.004236574417243E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0012117087516816216
70-71	7.410450216895866E-4
72-73	0.0
74-75	1.5021182872086217E-4
76-77	0.0029441518429288984
78-79	7.110026559454143E-4
80-81	0.0030743354278203123
82-83	0.0022231350650687596
84-85	0.006028501392663935
86-87	0.008171523482414901
88-89	0.00797124104412042
90-91	0.006078572002237556
92-93	0.009002695601337006
94-95	0.0018325843103945184
96-97	0.0048868914943853824
98-99	0.0019727820172006564
100	0.08538040344493805
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4992949.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.29165175730271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.70560521021319	16.61803285479526
2	16.40386292793688	8.29761577295815
3	6.834914500269003	5.185988319952268
4	3.4292545280534776	3.4692604524272794
5	1.9474877979173941	2.4627591593261515
6	1.2317073700027894	1.8691148321408244
7	0.8286687367704506	1.4670880778793527
8	0.5712116266896698	1.1557508433566022
9	0.4103346102517417	0.9340236059810025
>10	2.071868722311895	9.519245906375074
>50	0.21870215903975515	3.910391674058136
>100	0.2569220272127225	14.504611785981947
>500	0.04908412890963815	8.874541839811961
>1k	0.03807978387600926	17.089349733401747
>5k	0.0017416948966158079	2.802823070227231
>10k+	5.541756489232116E-4	1.8394020713269985
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	17963	0.35976734390837956	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	14178	0.2839604410139178	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	13268	0.2657347391291199	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	12521	0.250773640988522	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	12104	0.2424218633116421	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	11685	0.23403002914710327	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	10146	0.20320656189358233	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9069	0.18163614328926653	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8425	0.1687379542631018	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7513	0.15047219589064498	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7249	0.14518473951967065	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6910	0.13839516486148767	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6892	0.13803465647255758	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6629	0.13276722834541269	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	6517	0.1305240650365145	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6452	0.12922222918760037	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6184	0.12385465984130822	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5968	0.11952855917414738	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5952	0.1192081072728762	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5942	0.11900782483458172	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5873	0.11762587601034981	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5816	0.11648426611207123	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5693	0.1140207921210491	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5637	0.1128992104666	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5526	0.11067607540153125	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	5514	0.11043573647557786	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5504	0.11023545403728338	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5407	0.10829271438582688	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5309	0.10632994649054098	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.002824382944829E-5	0.0	0.0	0.0
5	0.0	1.6022595063558631E-4	0.0	0.0	0.0
6	0.0	1.6022595063558631E-4	0.0	0.0	2.002824382944829E-5
7	0.0	1.802541944650346E-4	0.0	0.0	4.005648765889658E-5
8	0.0	1.802541944650346E-4	0.0	0.0	4.005648765889658E-5
9	0.0	1.802541944650346E-4	0.0	0.0	4.005648765889658E-5
10-11	0.0	1.802541944650346E-4	0.0	0.0	4.005648765889658E-5
12-13	0.0	1.802541944650346E-4	0.0	0.0	1.0014121914724144E-4
14-15	0.0	1.9026831637975874E-4	0.0	0.0	1.0014121914724144E-4
16-17	0.0	2.0028243829448288E-4	0.0	0.0	1.1015534106196558E-4
18-19	0.0	2.4033892595337944E-4	0.0	0.0	1.2016946297668972E-4
20-21	0.0	3.605083889300692E-4	0.0	0.0	1.3018358489141386E-4
22-23	0.0	3.605083889300692E-4	0.0	4.005648765889658E-5	1.802541944650346E-4
24-25	0.0	3.705225108447933E-4	0.0	1.5021182872086215E-4	1.802541944650346E-4
26-27	0.0	3.805366327595175E-4	0.0	3.705225108447933E-4	1.802541944650346E-4
28-29	0.0	3.9055075467424164E-4	0.0	0.0014820900433791732	1.9026831637975874E-4
30-31	0.0	5.007060957362072E-4	0.0	0.006118628489896452	2.0028243829448288E-4
32-33	0.0	5.007060957362072E-4	0.0	0.01788522173969732	2.0028243829448288E-4
34-35	0.0	5.107202176509313E-4	0.0	0.03686198276809957	2.0028243829448288E-4
36-37	0.0	5.507767053098279E-4	0.0	0.06840646679948063	2.0028243829448288E-4
38-39	0.0	5.808190710540004E-4	0.0	0.12029964656158114	2.2031068212393116E-4
40-41	0.0	5.808190710540004E-4	0.0	0.17848169488612842	2.2031068212393116E-4
42-43	0.0	5.808190710540004E-4	0.0	0.2397480927604107	2.4033892595337944E-4
44-45	0.0	6.008473148834486E-4	0.0	0.3163761536518799	2.603671697828277E-4
46-47	0.0	6.208755587128969E-4	0.0	0.41444444956277343	2.80395413612276E-4
48-49	0.0	6.30889680627621E-4	0.0	0.527794295515536	3.2045190127117263E-4
50-51	0.0	6.409038025423453E-4	0.0	0.6492756084630547	3.2045190127117263E-4
52-53	0.0	6.609320463717935E-4	0.0	0.7681232073470008	3.404801451006209E-4
54-55	0.0	7.710873874337591E-4	0.0	0.905406804675954	3.404801451006209E-4
56-57	0.0	8.211579970073799E-4	0.0	1.0735338975022577	3.404801451006209E-4
58-59	0.0	9.81383947642966E-4	0.0	1.273796307552911	3.404801451006209E-4
60-61	0.0	9.81383947642966E-4	0.0	1.490121369154782	3.404801451006209E-4
62-63	0.0	9.81383947642966E-4	0.0	1.7147481378239595	3.404801451006209E-4
64-65	0.0	0.0010114263133871385	0.0	1.9507309207444339	3.705225108447933E-4
66-67	0.0	0.0010815251667902076	0.0	2.21950995293563	3.805366327595175E-4
68-69	0.0	0.0011015534106196558	0.0	2.528505698736358	3.805366327595175E-4
70-71	0.0	0.0011416098982785523	0.0	2.868294869424863	3.805366327595175E-4
72-73	0.0	0.001181666385937449	0.0	3.224306917615221	3.9055075467424164E-4
74-75	0.0	0.001181666385937449	0.0	3.5808597283889743	4.1057899850368987E-4
76-77	0.0	0.001181666385937449	0.0	3.9946632741492047	4.2059312041841404E-4
78-79	0.0	0.001261779361255242	0.0	4.442825272198855	4.2059312041841404E-4
80-81	0.0	0.0013619205804024836	0.0	4.929631766717424	4.2059312041841404E-4
82-83	0.0	0.0013719347023172079	0.0	5.441784003802162	4.2059312041841404E-4
84-85	0.0	0.0013819488242319319	0.0	5.941098136592222	4.2059312041841404E-4
86-87	0.0	0.00140197706806138	0.0	6.475541809059136	4.406213642478623E-4
88	0.0	0.00140197706806138	0.0	6.90571844414994	4.606496080773106E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9665	0.0	45.395573	1
GTATCAA	16775	0.0	38.40535	1
GGGCACC	5170	0.0	37.572247	94
CAACGCA	19165	0.0	32.17442	5
ATCAACG	19405	0.0	31.764038	3
GTACTAG	4450	0.0	31.596926	1
CTGTGCG	6540	0.0	31.335905	9
TAGTACT	6705	0.0	30.993155	4
TATCAAC	21440	0.0	29.955824	2
AACGCAG	22115	0.0	28.879951	6
GTGGTAT	2815	0.0	28.232023	1
TAGGCAT	5825	0.0	28.158869	5
ACTGTGC	7745	0.0	28.033684	8
GTACTGT	7710	0.0	27.551674	6
TACTGTG	8670	0.0	27.102842	7
ACGCAGA	23230	0.0	26.461988	7
TCAACGC	23515	0.0	26.391935	4
CGCAGAG	23440	0.0	26.204601	8
CTAGTAC	8150	0.0	26.132877	3
TATTCTC	3225	0.0	25.648895	5
>>END_MODULE