FastQCFastQC Report
Mon 16 Jan 2017
ERR1146433_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146433_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4345264
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN131880.30350284815836276No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC131660.3029965498068702No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA121460.27952271714676025No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT115170.2650471870063591No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC103510.23821337437725304No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA100190.23057287198200155No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC90430.20811163602487676No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC80930.18624875266497043No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT61400.14130326718928932No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT59320.1365164464115414No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC58580.13481344286561184No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG57270.13179866631808793No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC56030.12894498470058435No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT55060.12671266924173077No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA51510.11854285493355524No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT50350.11587328180750352No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG50240.11562013263175724No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC48830.11237522046991852No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC48520.11166180006554262No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC46490.1069900470949521No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA46460.10692100641065767No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG46360.10669087079634286No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT45060.10369910781025042No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA43890.10100652112276721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99300.034.3952061
GGGCACC40900.034.28351694
CAACGCA91050.033.2334445
GGTATCA60450.032.1521151
GTACTAG41700.031.7891481
ATCAACG98300.030.596473
AACGCAG112250.028.8575866
TAGGCAT51600.028.7744315
TATCAAC116950.028.6512932
TACCCCG3200.027.8979955
GCATCGT42200.027.4806488
ACGCAGA112350.026.7030097
GCTATTG50950.026.1707788
CGCAGAG114350.026.0312848
TAGTACT57400.025.785654
AGGGCTA52200.025.653335
CTATTGA52000.025.4629279
CTGTGCG57050.025.3488149
CTAGGCA60850.024.8642144
GGGCTAT57100.024.7472886
Produced by FastQC (version 0.11.5)