##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146433_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4345264
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.59662980200973	31.0	31.0	34.0	27.0	34.0
2	30.63806088651921	31.0	31.0	34.0	26.0	34.0
3	30.80534439334411	31.0	31.0	34.0	27.0	34.0
4	34.2595029899219	37.0	35.0	37.0	30.0	37.0
5	34.457251158962954	37.0	35.0	37.0	30.0	37.0
6	34.61801285261379	37.0	35.0	37.0	31.0	37.0
7	34.47609926577534	37.0	35.0	37.0	30.0	37.0
8	34.43386040525961	37.0	35.0	37.0	30.0	37.0
9	35.797707573118686	39.0	35.0	39.0	30.0	39.0
10-11	35.71931245144138	39.0	35.0	39.0	29.5	39.0
12-13	35.71984912769396	39.0	35.0	39.0	30.0	39.0
14-15	36.748480644674295	40.0	36.0	41.0	29.5	41.0
16-17	36.59448217645694	40.0	36.0	41.0	28.0	41.0
18-19	36.558257449950105	40.0	36.0	41.0	28.0	41.0
20-21	36.88863001189341	40.0	36.0	41.0	30.0	41.0
22-23	36.6096707817983	40.0	36.0	41.0	28.5	41.0
24-25	36.7313379578318	40.0	36.0	41.0	29.5	41.0
26-27	36.803654622596	40.0	36.0	41.0	30.0	41.0
28-29	36.63936874721536	40.0	35.5	41.0	29.5	41.0
30-31	36.58297171357137	40.0	35.0	41.0	29.5	41.0
32-33	36.68402437688481	40.0	35.5	41.0	30.0	41.0
34-35	36.73253408768719	40.0	35.0	41.0	30.0	41.0
36-37	36.67788159706752	40.0	35.0	41.0	30.0	41.0
38-39	36.51021215281742	39.0	35.0	41.0	29.0	41.0
40-41	36.47609466306305	39.0	35.0	41.0	29.0	41.0
42-43	36.3226893693916	39.0	35.0	41.0	29.0	41.0
44-45	36.15273870586459	39.0	35.0	41.0	28.0	41.0
46-47	36.02446698290369	39.0	35.0	41.0	28.0	41.0
48-49	35.90447519414241	39.0	34.5	41.0	28.0	41.0
50-51	35.68906768380471	38.0	34.0	40.0	27.5	41.0
52-53	35.514403497693124	38.0	34.0	40.0	27.0	41.0
54-55	35.327125693628744	38.0	34.0	40.0	27.0	41.0
56-57	35.18995267030956	37.5	34.0	40.0	27.0	41.0
58-59	34.94075089568781	37.0	33.0	40.0	26.5	41.0
60-61	34.696620734666524	36.5	33.0	40.0	26.0	41.0
62-63	34.434555184679226	36.0	33.0	39.5	26.0	41.0
64-65	34.14940012850772	35.5	33.0	39.0	26.0	41.0
66-67	33.86310244901115	35.0	32.0	39.0	26.0	41.0
68-69	33.57987684983007	35.0	32.0	38.5	25.0	40.5
70-71	33.50401678701225	35.0	32.0	38.0	26.0	40.0
72-73	33.29315744682026	35.0	32.0	37.0	25.5	40.0
74-75	33.023067758368654	35.0	32.0	37.0	25.0	39.5
76-77	32.781212142691444	35.0	32.0	36.0	25.0	39.0
78-79	32.56325599549302	35.0	32.0	36.0	25.0	39.0
80-81	32.3343306643739	35.0	31.5	36.0	24.5	38.0
82-83	32.09588416261935	35.0	31.0	35.0	24.0	37.0
84-85	31.911643228121466	35.0	31.0	35.0	24.0	37.0
86-87	31.73841336222609	35.0	31.0	35.0	24.0	36.5
88-89	31.72391850069409	35.0	32.0	35.0	24.0	36.0
90-91	31.64636084251728	35.0	31.5	35.0	24.0	36.0
92-93	31.505732908288195	35.0	31.0	35.0	24.0	36.0
94-95	31.4799249251599	35.0	31.0	35.0	24.0	35.5
96-97	31.391730168753845	35.0	31.0	35.0	23.0	35.0
98-99	31.31430679470799	35.0	31.0	35.0	23.0	35.0
100	30.837510678292503	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7913700773274925
1101	2	0.9408955213692529
1101	3	0.9126664947920169
1101	4	0.923837833922299
1101	5	0.8578087749272001
1101	6	0.5863364231128401
1101	7	0.6778658554853862
1101	8	0.718511495595962
1101	9	0.8452006414073523
1101	10-11	0.9043360344902425
1101	12-13	0.7963275941221113
1101	14-15	1.0929353831519322
1101	16-17	1.1668567005118007
1101	18-19	1.2985234198244413
1101	20-21	0.9691032212444171
1101	22-23	1.3191401388040305
1101	24-25	1.1845387348670968
1101	26-27	1.0471360527859517
1101	28-29	1.1954352083621131
1101	30-31	1.24867679209148
1101	32-33	1.0973551350292325
1101	34-35	0.99933372673474
1101	36-37	0.97131764896465
1101	38-39	1.0397374855124752
1101	40-41	0.947469939006865
1101	42-43	0.9587483066732503
1101	44-45	0.9677768553550123
1101	46-47	0.9258894028079254
1101	48-49	0.9568892031302596
1101	50-51	1.0262493644894377
1101	52-53	0.9775394229031917
1101	54-55	0.9788992894554838
1101	56-57	0.8826800022485131
1101	58-59	0.7856358813454705
1101	60-61	0.8091981667773567
1101	62-63	0.7973792667747901
1101	64-65	0.7822905921530037
1101	66-67	0.8416836047169625
1101	68-69	0.8242571091524766
1101	70-71	0.7441395484246698
1101	72-73	0.7263612371072838
1101	74-75	0.765154835916583
1101	76-77	0.7162129637907455
1101	78-79	0.7091782505068664
1101	80-81	0.7504362159865892
1101	82-83	0.7089818476463918
1101	84-85	0.7127947042400855
1101	86-87	0.6914902949569459
1101	88-89	0.6885868011407261
1101	90-91	0.6821471387482276
1101	92-93	0.7076103267022447
1101	94-95	0.6739413151849085
1101	96-97	0.6550255335320472
1101	98-99	0.6196595233922011
1101	100	0.681196451640389
1102	1	0.7922850967195103
1102	2	0.9202967966106002
1102	3	0.893360624507082
1102	4	0.9689568407850189
1102	5	0.8167297584875328
1102	6	0.5747056875395344
1102	7	0.5999023673962967
1102	8	0.6564213963135543
1102	9	0.867619815227151
1102	10-11	0.8567947435409309
1102	12-13	0.8004115374417324
1102	14-15	1.0001680800153565
1102	16-17	1.1549092643401977
1102	18-19	1.1936339078166682
1102	20-21	0.9413082086035658
1102	22-23	1.1918573452282502
1102	24-25	1.061675045425794
1102	26-27	0.8956798054171244
1102	28-29	1.112613007258588
1102	30-31	1.1278189530204585
1102	32-33	1.0225493029433608
1102	34-35	0.9447561364466992
1102	36-37	0.8658348527676765
1102	38-39	0.9127829801168232
1102	40-41	0.9001115720216006
1102	42-43	0.9366884278042349
1102	44-45	0.9976888792372591
1102	46-47	0.9476932360758923
1102	48-49	0.8860083647071804
1102	50-51	0.985943519457436
1102	52-53	0.8807870305070722
1102	54-55	0.9457723109757765
1102	56-57	0.7867476909878377
1102	58-59	0.8218725672139584
1102	60-61	0.8721586932403582
1102	62-63	0.7804912053724351
1102	64-65	0.7765839878067453
1102	66-67	0.7963278116133097
1102	68-69	0.8573606727254628
1102	70-71	0.771098656887304
1102	72-73	0.7208029538083309
1102	74-75	0.7175520908264374
1102	76-77	0.6964791521220377
1102	78-79	0.7083834223083514
1102	80-81	0.7713720994190325
1102	82-83	0.7404512425252889
1102	84-85	0.7239694484901698
1102	86-87	0.7563688459114637
1102	88-89	0.7392671280479881
1102	90-91	0.7618939956366333
1102	92-93	0.7994689236810402
1102	94-95	0.7018871262181428
1102	96-97	0.7216883323438168
1102	98-99	0.7719996235536577
1102	100	0.8178814890195305
1103	1	0.5976462091368084
1103	2	0.7492691427060016
1103	3	0.7506316670407998
1103	4	0.7050227989321698
1103	5	0.6942933857862599
1103	6	0.532285019159005
1103	7	0.5935323908625989
1103	8	0.6168369102242508
1103	9	0.7313629317038703
1103	10-11	0.8041396955809148
1103	12-13	0.6702325094734931
1103	14-15	0.8386106854897122
1103	16-17	1.0501787403067269
1103	18-19	1.122964786517862
1103	20-21	0.8190554179947185
1103	22-23	1.0294604652220087
1103	24-25	0.9226347997220969
1103	26-27	0.7973406758281385
1103	28-29	1.067473713540771
1103	30-31	1.0843096750391652
1103	32-33	0.8407154273450317
1103	34-35	0.8893984058047835
1103	36-37	0.7751934491440906
1103	38-39	0.9084230664583757
1103	40-41	0.8509416851532592
1103	42-43	0.8988750257961726
1103	44-45	0.9172391804085152
1103	46-47	0.8652228459349587
1103	48-49	0.8875111612743893
1103	50-51	0.9112603918111972
1103	52-53	0.9595033278946019
1103	54-55	0.8957030328914186
1103	56-57	0.8570721932710228
1103	58-59	0.8380084669175858
1103	60-61	0.7965138530888254
1103	62-63	0.7303552580018717
1103	64-65	0.7738048125863344
1103	66-67	0.8184290020626648
1103	68-69	0.8148282447202462
1103	70-71	0.713806228650192
1103	72-73	0.627348074099686
1103	74-75	0.7083240309799734
1103	76-77	0.6478843281064002
1103	78-79	0.6369493756243969
1103	80-81	0.6995274085809413
1103	82-83	0.6633987786281565
1103	84-85	0.5943493796823169
1103	86-87	0.55831517037252
1103	88-89	0.5711043210069384
1103	90-91	0.5818047940641407
1103	92-93	0.6728307755798895
1103	94-95	0.6405082250181628
1103	96-97	0.6161592262082465
1103	98-99	0.6513340128597775
1103	100	0.6430707557153532
1104	1	0.6320911638208067
1104	2	0.7864238839448596
1104	3	0.6920336028832601
1104	4	0.801120106140921
1104	5	0.6999358983371877
1104	6	0.4580031112443521
1104	7	0.4862737836339548
1104	8	0.48407786973253764
1104	9	0.7447781819150947
1104	10-11	0.7414084882764271
1104	12-13	0.6619160077375525
1104	14-15	0.8490294097354294
1104	16-17	0.9746321647070104
1104	18-19	0.9724229238254836
1104	20-21	0.7258587340431859
1104	22-23	1.0328121037998699
1104	24-25	0.9131728497355098
1104	26-27	0.7750382610894135
1104	28-29	0.926695972088524
1104	30-31	1.034096238008658
1104	32-33	0.8436961835440826
1104	34-35	0.7233435309824898
1104	36-37	0.7227491083095146
1104	38-39	0.8706402379498073
1104	40-41	0.7393328301500404
1104	42-43	0.704123861897294
1104	44-45	0.7550870524452407
1104	46-47	0.7160851908325441
1104	48-49	0.7039927344374561
1104	50-51	0.786751912903398
1104	52-53	0.7772499291945323
1104	54-55	0.7080595054884355
1104	56-57	0.6316660677170134
1104	58-59	0.6857873493800213
1104	60-61	0.688352967408278
1104	62-63	0.6337221617136635
1104	64-65	0.6418027659409802
1104	66-67	0.7451007144538409
1104	68-69	0.7408568435071032
1104	70-71	0.6229235326437106
1104	72-73	0.6298744132141891
1104	74-75	0.6398481680943959
1104	76-77	0.6356629168006123
1104	78-79	0.6286645260625505
1104	80-81	0.6738195127760278
1104	82-83	0.65743787449248
1104	84-85	0.6008515247310022
1104	86-87	0.6811176759450994
1104	88-89	0.648504474955903
1104	90-91	0.6014274523889895
1104	92-93	0.6716078977759352
1104	94-95	0.6268312192120042
1104	96-97	0.6240892109683429
1104	98-99	0.7139025888156176
1104	100	0.8352037328742448
1105	1	0.4991186589683174
1105	2	0.6798598535105889
1105	3	0.6990021730734242
1105	4	0.748074282909748
1105	5	0.7055761854112603
1105	6	0.42790246954125166
1105	7	0.5194212415157153
1105	8	0.5451274540760878
1105	9	0.6300767957604094
1105	10-11	0.6219012470989824
1105	12-13	0.6224177980650367
1105	14-15	0.8126652337264204
1105	16-17	0.9624212959038587
1105	18-19	0.9189081379740358
1105	20-21	0.7663782333731604
1105	22-23	0.9501525608838151
1105	24-25	0.8129178349189985
1105	26-27	0.6593119588603713
1105	28-29	0.9306991262077915
1105	30-31	0.946159546181562
1105	32-33	0.7623129127232673
1105	34-35	0.7740824350102287
1105	36-37	0.6073459241427983
1105	38-39	0.7043562014369726
1105	40-41	0.6275531919628392
1105	42-43	0.64540403454928
1105	44-45	0.6746306765136012
1105	46-47	0.5811462182482643
1105	48-49	0.6787145688345362
1105	50-51	0.739596556340274
1105	52-53	0.7017922019908838
1105	54-55	0.6785179255967364
1105	56-57	0.7224212193875772
1105	58-59	0.701257676223797
1105	60-61	0.7042127671091833
1105	62-63	0.6649093577853975
1105	64-65	0.6453873495247109
1105	66-67	0.7065004207140788
1105	68-69	0.6513898253370343
1105	70-71	0.6291923123026208
1105	72-73	0.631872730815779
1105	74-75	0.6544628979954084
1105	76-77	0.5771962802677422
1105	78-79	0.608814405009312
1105	80-81	0.6602613005822846
1105	82-83	0.665424377745893
1105	84-85	0.5855700845599792
1105	86-87	0.6297723616211037
1105	88-89	0.6481144300746742
1105	90-91	0.6336353337153611
1105	92-93	0.7153247203785256
1105	94-95	0.5833909432762177
1105	96-97	0.5776191608547165
1105	98-99	0.627764006157495
1105	100	0.7463052742741105
1106	1	0.6061460398391887
1106	2	0.5807685406367611
1106	3	0.6008618128639469
1106	4	0.7103083567458413
1106	5	0.5902421340465551
1106	6	0.3349247153467303
1106	7	0.3429585378906168
1106	8	0.4325493966932683
1106	9	0.5402255063762169
1106	10-11	0.4626335074230923
1106	12-13	0.36805886270015264
1106	14-15	0.639626938427682
1106	16-17	0.7819551065788275
1106	18-19	0.740413682089077
1106	20-21	0.5305125886283975
1106	22-23	0.8614357149726501
1106	24-25	0.672338276955962
1106	26-27	0.5342827723776153
1106	28-29	0.7198047667918388
1106	30-31	0.7737769811769013
1106	32-33	0.6753685202146755
1106	34-35	0.5784188550884224
1106	36-37	0.4496576011059261
1106	38-39	0.6211078051573935
1106	40-41	0.5582733256325412
1106	42-43	0.5696077592711504
1106	44-45	0.5321566963726951
1106	46-47	0.4968932912609887
1106	48-49	0.5185688646458999
1106	50-51	0.6042463492698644
1106	52-53	0.6063433621645444
1106	54-55	0.6083863516158061
1106	56-57	0.5885473875052298
1106	58-59	0.5923424127027772
1106	60-61	0.5406761312704731
1106	62-63	0.5586139760964173
1106	64-65	0.5743884542221167
1106	66-67	0.5437412315803982
1106	68-69	0.46526360894098673
1106	70-71	0.5199276339157137
1106	72-73	0.5188929313730029
1106	74-75	0.5295288418906523
1106	76-77	0.5311042405846678
1106	78-79	0.516513952772435
1106	80-81	0.5599129610078748
1106	82-83	0.4845942680925006
1106	84-85	0.4340406799466656
1106	86-87	0.5081559637334117
1106	88-89	0.48851619188488726
1106	90-91	0.44151929396556255
1106	92-93	0.44020132519618116
1106	94-95	0.47453975834890727
1106	96-97	0.4871457824381409
1106	98-99	0.44392276219010185
1106	100	0.427451135586967
1107	1	0.3546229645179224
1107	2	0.5919684361660487
1107	3	0.4701782179420704
1107	4	0.6649739357641664
1107	5	0.5547837688014212
1107	6	0.24499815366842626
1107	7	0.25187578367561514
1107	8	0.3685459978657377
1107	9	0.44365808394395145
1107	10-11	0.441481806068154
1107	12-13	0.3339684328878789
1107	14-15	0.49391486994289835
1107	16-17	0.6383155993558773
1107	18-19	0.7255338851716715
1107	20-21	0.46707411693608236
1107	22-23	0.7307316273796047
1107	24-25	0.49759510814048014
1107	26-27	0.4243430548014828
1107	28-29	0.6151590627968915
1107	30-31	0.59535812421354
1107	32-33	0.5591335819970951
1107	34-35	0.45924078105539934
1107	36-37	0.40714302902996735
1107	38-39	0.4167831015400978
1107	40-41	0.2413748792814232
1107	42-43	0.413207847330078
1107	44-45	0.40647642351355273
1107	46-47	0.35702893553821724
1107	48-49	0.3715569563966028
1107	50-51	0.42628680167040045
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2214	4	-0.7322498423057482
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2214	100	-0.9899044646103583
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2215	4	-2.0385175559030984
2215	5	-1.493838596688434
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2215	9	-1.8119419341536371
2215	10-11	-1.3650671485942354
2215	12-13	-1.3335217937396848
2215	14-15	-3.7580091416664345
2215	16-17	-3.5128588851874767
2215	18-19	-2.381518420009307
2215	20-21	-1.870903683050571
2215	22-23	-3.30947997111722
2215	24-25	-5.5848016000685305
2215	26-27	-3.6175416303787884
2215	28-29	-1.790523088809877
2215	30-31	-3.8401665559316527
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2215	100	-3.059891609302422
2216	1	-1.0533473759591487
2216	2	-0.9769336130131201
2216	3	-1.203039397640044
2216	4	-1.1885181745910955
2216	5	-1.2783171570186695
2216	6	-1.1686998479608377
2216	7	-1.3306875138158318
2216	8	-1.1460030247989437
2216	9	-1.483534927917674
2216	10-11	-1.446393153493105
2216	12-13	-1.6512719196693268
2216	14-15	-2.0556602455574335
2216	16-17	-1.745507165648668
2216	18-19	-3.7662067155713927
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2216	22-23	-1.555884369521813
2216	24-25	-1.8020345315114312
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2216	28-29	-1.5934099730585984
2216	30-31	-1.803802062493311
2216	32-33	-1.8573979590129426
2216	34-35	-2.0225241414980957
2216	36-37	-1.98631799044243
2216	38-39	-2.156244083394121
2216	40-41	-2.1298685985193657
2216	42-43	-2.1448206320478747
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2216	54-55	-1.8617294925313956
2216	56-57	-1.9569071212654237
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2216	60-61	-1.9528525525864922
2216	62-63	-2.0200904322777475
2216	64-65	-2.0361771903911148
2216	66-67	-2.0193826551000527
2216	68-69	-2.022788442601467
2216	70-71	-2.1215759059481485
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2216	74-75	-2.141789407543289
2216	76-77	-1.9444397727825944
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2216	80-81	-1.9627470866465586
2216	82-83	-2.136100728191863
2216	84-85	-2.013722136907546
2216	86-87	-1.9757830399137895
2216	88-89	-1.8924308673697006
2216	90-91	-1.9563205634714222
2216	92-93	-1.820882196609226
2216	94-95	-1.796683768130908
2216	96-97	-1.7839239614830262
2216	98-99	-1.7466992134061279
2216	100	-1.790617818720687
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	17784.0
1	3051.0
2	486.0
3	470.0
4	416.0
5	256.0
6	227.0
7	337.0
8	684.0
9	1125.0
10	1836.0
11	3171.0
12	5306.0
13	7300.0
14	9380.0
15	11930.0
16	14408.0
17	17195.0
18	19799.0
19	22750.0
20	25775.0
21	29956.0
22	34625.0
23	39787.0
24	45086.0
25	50237.0
26	55983.0
27	63913.0
28	76503.0
29	90143.0
30	107283.0
31	128114.0
32	158311.0
33	202485.0
34	278906.0
35	377635.0
36	555303.0
37	864983.0
38	756904.0
39	265097.0
40	324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.166436691406055	21.26335917610588	12.781037826901153	23.78916630558691
2	15.202361578577506	19.89694677663942	41.295909309464804	23.604782335318266
3	18.38250749978205	26.9394391298426	29.1084734529799	25.569579917395448
4	12.027302071886083	17.05045417520502	40.3806345898227	30.541609163086203
5	13.01385424806826	38.56071695115477	35.20334826211135	13.22208053866563
6	29.48110769486936	38.763400140838066	18.481168979085894	13.274323185206685
7	26.694250068175933	31.980678049291605	23.870991468929468	17.45408041360299
8	25.780515956819322	35.448148722136	20.199827443832504	18.571507877212177
9	25.953763871451617	15.341954459742976	21.80243217276861	36.901849496036796
10-11	25.39256761477981	26.10904342329511	27.869061451743594	20.62932751018149
12-13	25.69893087609208	23.94648758241583	27.754858302733115	22.599723238758976
14-15	23.118353393580204	25.153001147660436	25.215329485896476	26.513315972862877
16-17	22.06140060647259	27.695832201538067	26.560697466614158	23.682069725375182
18-19	21.54947229795638	28.255415840686176	28.100851060679254	22.094260800678185
20-21	23.25012751048744	27.339370622809923	27.04188278474807	22.36861908195457
22-23	23.635847883406527	27.02882006709183	26.816586568844752	22.518745480656893
24-25	23.080106924603534	27.1803448073224	27.500683664106457	22.238864603967613
26-27	22.86600214311448	27.433183808787465	26.592419065044627	23.108394983053433
28-29	22.100461648947164	27.83558204976389	27.165819217240568	22.898137084048376
30-31	22.928660716010405	27.413502597267254	27.035846556210664	22.621990130511684
32-33	23.26856851766942	27.690839403275664	26.12582142594408	22.914770653110835
34-35	23.285344559917558	27.51124331983225	27.025498123375506	22.177913996874686
36-37	23.01328114288293	27.14728779936374	26.750965120295096	23.08846593745824
38-39	23.10093895627305	27.191958480454993	27.301761757777975	22.40534080549398
40-41	22.78451575641093	26.42302421831143	27.900958578824632	22.89150144645301
42-43	22.74163680382479	26.68052385830308	27.215463441148085	23.362375896724046
44-45	22.887266008695146	27.587167264370283	27.002233838687367	22.523332888247204
46-47	22.84070978925745	27.27528435052191	26.590693301312395	23.293312558908248
48-49	23.474464598195325	26.82805373127146	26.88560080767177	22.811880862861443
50-51	23.150099446818395	26.769091336511558	26.7486605452962	23.332148671373847
52-53	22.565475666469354	27.379246225708897	26.6421392355877	23.413138872234054
54-55	22.62105819701813	27.23178038703915	26.64884995435914	23.498311461583583
56-57	23.54436219315838	26.732335050812374	26.45645334043137	23.266849415597886
58-59	22.632518681524118	27.036828117021294	27.261420561673148	23.069232639781436
60-61	22.72976558676302	27.618951330938263	26.567866913924398	23.083416168374324
62-63	23.04257502131238	26.954533203936055	26.21571945620754	23.787172318544027
64-65	22.2581589295031	27.180991669568417	26.649260024918416	23.91158937601007
66-67	23.00323898039271	26.74693995074492	26.56087701765319	23.688944051209184
68-69	22.42668506843853	26.892227218377336	26.804418461660934	23.876669251523204
70-71	22.453824291620784	26.545289890910368	27.038684552382215	23.962201265086634
72-73	23.00033365372112	26.725639923310545	27.26731817838951	23.00670824457882
74-75	23.160949020713005	27.053733123151453	26.835001900753152	22.950315955382393
76-77	22.82287148899588	27.040379482602383	26.526304002968644	23.610445025433098
78-79	22.69333559507248	26.955225086050422	26.629722098830673	23.72171722004643
80-81	22.371589904389904	27.232457205310745	27.24707906876776	23.148873821531595
82-83	23.18610965612848	26.390748747429615	28.09159773750715	22.331543858934754
84-85	23.40864584746911	26.924579547838007	26.747332120216356	22.919442484476534
86-87	22.175394368039235	27.499550024770397	27.25356356592053	23.071492041269835
88-89	23.105471749926693	26.322661497144505	27.07687724867232	23.494989504256477
90-91	22.884655637231948	26.383357578729083	27.297589147060435	23.434397636978535
92-93	23.08266625463521	26.651376824603396	27.17724050540716	23.088716415354238
94-95	22.035872014253112	26.64578235981575	27.73192843841732	23.586417187513817
96-97	22.43475201449032	26.603116377959314	27.21577646853779	23.74635513901257
98-99	21.80252008206758	27.324956328156315	27.16496293637668	23.707560653399423
100	22.650341869563253	26.57750245588596	26.456813867809814	24.31534180674097
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14898.0
1	8788.5
2	2465.5
3	1761.5
4	931.0
5	519.0
6	416.5
7	405.0
8	425.0
9	395.5
10	374.5
11	363.5
12	350.5
13	366.0
14	402.0
15	553.0
16	671.5
17	762.0
18	953.0
19	1184.5
20	1513.5
21	1922.0
22	2610.5
23	3545.5
24	4792.0
25	6580.0
26	9241.5
27	12374.5
28	17127.5
29	26387.0
30	38827.0
31	51035.5
32	66550.0
33	81310.0
34	93003.0
35	105667.5
36	117831.0
37	138170.5
38	146112.5
39	135876.5
40	125688.5
41	116167.0
42	114797.5
43	121976.5
44	132599.0
45	155016.5
46	177125.5
47	190186.0
48	198860.0
49	195054.0
50	185314.5
51	186869.5
52	187149.0
53	179815.0
54	170899.5
55	149463.5
56	131727.5
57	110594.5
58	89479.0
59	83790.5
60	69743.5
61	50437.0
62	38069.0
63	31004.0
64	22930.5
65	12414.0
66	7400.0
67	5148.5
68	3618.0
69	2697.0
70	2071.0
71	1628.0
72	1279.5
73	1043.0
74	847.5
75	676.5
76	474.0
77	304.0
78	229.5
79	151.0
80	91.0
81	70.0
82	57.0
83	44.0
84	38.5
85	33.5
86	29.0
87	28.0
88	19.5
89	13.5
90	13.5
91	12.0
92	9.5
93	8.5
94	8.5
95	4.5
96	3.5
97	4.0
98	3.0
99	2.5
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9976839151775357
2	0.4451743323305558
3	0.4787971455819486
4	0.46420654763439
5	0.4308368835587435
6	0.48671381071437775
7	0.5037898732965362
8	0.5070808125812378
9	0.5207278545101057
10-11	0.5340527065789328
12-13	0.5322346352258459
14-15	0.5291393112133118
16-17	0.5344209235618365
18-19	0.5322576487872773
20-21	0.5309343690049673
22-23	0.49452691482036537
24-25	0.5285294518353775
26-27	0.508979431399335
28-29	0.5152851472315606
30-31	0.518898276376303
32-33	0.5200604612285928
34-35	0.5325798386473182
36-37	0.5502887741688423
38-39	0.5395299341996251
40-41	0.5444548363459619
42-43	0.5422225208871084
44-45	0.5523945150398226
46-47	0.5505994572481672
48-49	0.5227760614775074
50-51	0.537504740793655
52-53	0.5299677994248451
54-55	0.540335408849727
56-57	0.5279426060188749
58-59	0.5342138015089531
60-61	0.5274363076673823
62-63	0.5214642884759131
64-65	0.5329710691916533
66-67	0.5309343690049673
68-69	0.550967674231071
70-71	0.5354680406069688
72-73	0.5389315816024066
74-75	0.5360548864234717
76-77	0.5234894818818834
78-79	0.5547534050865494
80-81	0.5286099993003878
82-83	0.5282072619753369
84-85	0.5371250170300355
86-87	0.844448116386024
88-89	1.1111868001575969
90-91	1.1019928823657206
92-93	1.1013715162070705
94-95	1.1069062777313416
96-97	1.0994728973889734
98-99	1.0951348410591395
100	1.0914411644493867
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4345264.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.3301123650695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9077239200438	24.96682855266664
2	12.724003511914312	8.481849335712859
3	4.87318814749043	4.872717255959428
4	2.357675459668839	3.1432635196451715
5	1.280065910016956	2.1332370307780044
6	0.8267787853557677	1.6533977890178049
7	0.5500312484235144	1.2832822319978632
8	0.3710891282433415	0.9894753873445008
9	0.2844102899968626	0.8531484231039688
>10	1.404502230809102	8.709396626023839
>50	0.16917337766916696	3.99684192647057
>100	0.19069298370305524	13.92648952142817
>500	0.037130572908598065	8.711545693548626
>1k	0.02236116286532822	13.115625025724349
>5k	7.591752824648469E-4	1.5437832826928422
>10k+	4.1409560861718926E-4	1.6191183978856403
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	13188	0.30350284815836276	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	13166	0.3029965498068702	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12146	0.27952271714676025	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	11517	0.2650471870063591	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10351	0.23821337437725304	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10019	0.23057287198200155	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	9043	0.20811163602487676	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8093	0.18624875266497043	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6140	0.14130326718928932	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5932	0.1365164464115414	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5858	0.13481344286561184	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	5727	0.13179866631808793	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5603	0.12894498470058435	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5506	0.12671266924173077	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5151	0.11854285493355524	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5035	0.11587328180750352	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5024	0.11562013263175724	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4883	0.11237522046991852	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4852	0.11166180006554262	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4649	0.1069900470949521	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4646	0.10692100641065767	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4636	0.10669087079634286	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4506	0.10369910781025042	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4389	0.10100652112276721	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.602712286296068E-5
2	0.0	0.0	0.0	0.0	4.602712286296068E-5
3	0.0	0.0	0.0	0.0	4.602712286296068E-5
4	0.0	0.0	0.0	2.301356143148034E-5	4.602712286296068E-5
5	0.0	0.0	0.0	2.301356143148034E-5	4.602712286296068E-5
6	0.0	0.0	0.0	2.301356143148034E-5	4.602712286296068E-5
7	0.0	0.0	0.0	2.301356143148034E-5	4.602712286296068E-5
8	0.0	0.0	0.0	2.301356143148034E-5	6.904068429444103E-5
9	0.0	0.0	0.0	2.301356143148034E-5	6.904068429444103E-5
10-11	0.0	0.0	0.0	2.301356143148034E-5	6.904068429444103E-5
12-13	0.0	0.0	0.0	2.301356143148034E-5	1.3808136858888204E-4
14-15	0.0	0.0	0.0	2.301356143148034E-5	1.956152721675829E-4
16-17	0.0	0.0	0.0	2.301356143148034E-5	2.3013561431480342E-4
18-19	0.0	0.0	0.0	4.602712286296069E-5	2.3013561431480342E-4
20-21	0.0	0.0	0.0	6.904068429444103E-5	2.416423950305436E-4
22-23	0.0	0.0	0.0	8.05474650101812E-5	2.7616273717776413E-4
24-25	0.0	0.0	0.0	1.4958814930462225E-4	2.7616273717776413E-4
26-27	0.0	0.0	0.0	3.912305443351658E-4	3.106830793249846E-4
28-29	0.0	0.0	0.0	0.0012887594401628992	3.4520342147220515E-4
30-31	0.0	1.150678071574017E-5	0.0	0.005016956392062715	3.4520342147220515E-4
32-33	0.0	2.301356143148034E-5	0.0	0.014740186096863159	3.6821698290368546E-4
34-35	0.0	2.301356143148034E-5	0.0	0.030608036703868857	3.912305443351658E-4
36-37	0.0	2.301356143148034E-5	0.0	0.0564867865335685	3.912305443351658E-4
38-39	0.0	2.301356143148034E-5	0.0	0.10161638050070146	3.912305443351658E-4
40-41	0.0	2.301356143148034E-5	0.0	0.15223470886924245	3.912305443351658E-4
42-43	0.0	2.301356143148034E-5	0.0	0.20555713070598242	3.912305443351658E-4
44-45	0.0	2.301356143148034E-5	0.0	0.2705474281884829	4.02737325050906E-4
46-47	0.0	2.301356143148034E-5	0.0	0.3550187054227315	4.257508864823863E-4
48-49	0.0	2.301356143148034E-5	0.0	0.4543337297802849	4.487644479138667E-4
50-51	0.0	2.301356143148034E-5	0.0	0.5639588296591416	4.6027122862960684E-4
52-53	0.0	2.301356143148034E-5	0.0	0.6721916090713936	5.753390357870086E-4
54-55	0.0	2.301356143148034E-5	0.0	0.7935996524031681	5.983525972184889E-4
56-57	0.0	2.301356143148034E-5	0.0	0.9451669679908976	5.983525972184889E-4
58-59	0.0	2.301356143148034E-5	0.0	1.1343499497383818	5.983525972184889E-4
60-61	0.0	2.301356143148034E-5	0.0	1.3328649306463314	6.213661586499693E-4
62-63	0.0	2.301356143148034E-5	0.0	1.5437612076044172	6.673932815129299E-4
64-65	0.0	2.301356143148034E-5	0.0	1.7627352446249525	7.019136236601504E-4
66-67	0.0	2.301356143148034E-5	0.0	2.0134910099823626	7.249271850916308E-4
68-69	0.0	2.301356143148034E-5	0.0	2.298732597144846	7.364339658073709E-4
70-71	0.0	2.301356143148034E-5	0.0	2.6235690167501904	7.824610886703316E-4
72-73	0.0	2.301356143148034E-5	0.0	2.960430022203484	8.515017729647727E-4
74-75	0.0	2.301356143148034E-5	0.0	3.292941464546228	9.320492379749539E-4
76-77	0.0	2.301356143148034E-5	0.0	3.682515032458327	9.895831415536547E-4
78-79	0.0	2.301356143148034E-5	0.0	4.104342106716646	0.001012596702985135
80-81	0.0	2.301356143148034E-5	0.0	4.56494703198701	0.0010241034837008753
82-83	0.0	2.301356143148034E-5	0.0	5.04230582997949	0.0011276645101425367
84-85	0.0	2.301356143148034E-5	0.0	5.507145250553246	0.0011276645101425367
86-87	0.0	2.301356143148034E-5	0.0	6.006148303072035	0.0011736916330054976
88	0.0	2.301356143148034E-5	0.0	6.41028945537026	0.0012197187558684582
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9930	0.0	34.395206	1
GGGCACC	4090	0.0	34.283516	94
CAACGCA	9105	0.0	33.233444	5
GGTATCA	6045	0.0	32.152115	1
GTACTAG	4170	0.0	31.789148	1
ATCAACG	9830	0.0	30.59647	3
AACGCAG	11225	0.0	28.857586	6
TAGGCAT	5160	0.0	28.774431	5
TATCAAC	11695	0.0	28.651293	2
TACCCCG	320	0.0	27.897995	5
GCATCGT	4220	0.0	27.480648	8
ACGCAGA	11235	0.0	26.703009	7
GCTATTG	5095	0.0	26.170778	8
CGCAGAG	11435	0.0	26.031284	8
TAGTACT	5740	0.0	25.78565	4
AGGGCTA	5220	0.0	25.65333	5
CTATTGA	5200	0.0	25.462927	9
CTGTGCG	5705	0.0	25.348814	9
CTAGGCA	6085	0.0	24.864214	4
GGGCTAT	5710	0.0	24.747288	6
>>END_MODULE