##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146432_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5455200 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.583454868749083 31.0 31.0 34.0 27.0 34.0 2 30.642898152221733 31.0 31.0 34.0 27.0 34.0 3 30.894981485555068 31.0 31.0 34.0 27.0 34.0 4 34.39091399032116 37.0 35.0 37.0 30.0 37.0 5 34.58201917436574 37.0 35.0 37.0 31.0 37.0 6 34.703931844845286 37.0 35.0 37.0 31.0 37.0 7 34.633755865962755 37.0 35.0 37.0 31.0 37.0 8 34.56107273793811 37.0 35.0 37.0 30.0 37.0 9 35.95910654054847 39.0 35.0 39.0 30.0 39.0 10-11 35.88201954098841 39.0 35.0 39.0 30.0 39.0 12-13 35.85859326880774 39.0 35.0 39.0 30.0 39.0 14-15 36.88955299530723 40.0 36.0 41.0 30.0 41.0 16-17 36.72751631470891 40.0 36.0 41.0 29.5 41.0 18-19 36.682403486581606 40.0 36.0 41.0 28.5 41.0 20-21 37.01585074791025 40.0 36.0 41.0 30.0 41.0 22-23 36.73063150755243 40.0 36.0 41.0 29.5 41.0 24-25 36.840230514004986 40.0 36.0 41.0 30.0 41.0 26-27 36.905938645695855 40.0 36.0 41.0 30.0 41.0 28-29 36.753720211907904 40.0 36.0 41.0 29.5 41.0 30-31 36.70321234785159 40.0 35.5 41.0 30.0 41.0 32-33 36.809313040768444 40.0 35.5 41.0 30.0 41.0 34-35 36.86075790071858 40.0 36.0 41.0 30.0 41.0 36-37 36.79186363469717 40.0 35.0 41.0 30.0 41.0 38-39 36.61811693430121 39.5 35.0 41.0 30.0 41.0 40-41 36.575584946473086 39.0 35.0 41.0 30.0 41.0 42-43 36.41573535342425 39.0 35.0 41.0 29.0 41.0 44-45 36.21448067898519 39.0 35.0 41.0 28.5 41.0 46-47 36.07298137556826 39.0 35.0 41.0 28.0 41.0 48-49 35.93733877768001 38.0 34.5 40.5 28.0 41.0 50-51 35.70003464584249 38.0 34.0 40.0 27.5 41.0 52-53 35.49700570098255 38.0 34.0 40.0 27.5 41.0 54-55 35.2929351811116 37.5 34.0 40.0 27.0 41.0 56-57 35.12782858556973 37.0 33.5 40.0 27.0 41.0 58-59 34.84819859950139 36.5 33.0 40.0 27.0 41.0 60-61 34.59813095761842 36.0 33.0 39.5 26.5 41.0 62-63 34.32190854597448 35.5 33.0 39.0 26.0 41.0 64-65 34.02475197976243 35.0 33.0 39.0 26.0 41.0 66-67 33.739229175832236 35.0 32.0 38.5 26.0 40.5 68-69 33.456776378501246 35.0 32.0 38.0 26.0 40.0 70-71 33.41113304736765 35.0 32.0 37.0 26.0 40.0 72-73 33.215150315295496 35.0 32.0 37.0 26.0 40.0 74-75 32.95944346678399 35.0 32.0 36.0 25.0 39.0 76-77 32.74301263015105 35.0 32.0 36.0 25.0 39.0 78-79 32.538553123625164 35.0 32.0 36.0 25.0 39.0 80-81 32.33770081756856 35.0 32.0 35.0 24.5 37.5 82-83 32.10428233611967 35.0 31.0 35.0 24.0 37.0 84-85 31.93920937820795 35.0 31.0 35.0 24.0 37.0 86-87 31.780997213667696 35.0 31.0 35.0 24.0 36.0 88-89 31.793388785012468 35.0 32.0 35.0 24.0 36.0 90-91 31.720421341105734 35.0 32.0 35.0 24.0 36.0 92-93 31.5802361966564 35.0 32.0 35.0 24.0 36.0 94-95 31.572862131544213 35.0 32.0 35.0 24.0 35.0 96-97 31.481894247690278 35.0 31.5 35.0 24.0 35.0 98-99 31.40880426015545 35.0 31.0 35.0 23.5 35.0 100 30.915254252822994 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8352926427811909 1101 2 0.9707153322502187 1101 3 0.9579076345170279 1101 4 1.0504359878047609 1101 5 0.9366184782895317 1101 6 0.6858109761169828 1101 7 0.7332539311171757 1101 8 0.7344653373309598 1101 9 0.9927186445739338 1101 10-11 0.9944074899536517 1101 12-13 0.8382475392635911 1101 14-15 1.097943537964582 1101 16-17 1.2498128277489258 1101 18-19 1.3477705335938452 1101 20-21 1.0066724108228158 1101 22-23 1.3375170686281024 1101 24-25 1.1720076043870833 1101 26-27 1.0554296979370363 1101 28-29 1.262267429658948 1101 30-31 1.2376783148919586 1101 32-33 1.084221935233245 1101 34-35 1.0343366530563785 1101 36-37 1.0361363415428286 1101 38-39 1.1342495012769547 1101 40-41 1.0337938459042704 1101 42-43 1.046313078289863 1101 44-45 1.0783936043404552 1101 46-47 1.093271257658209 1101 48-49 1.0449945968608034 1101 50-51 1.046988527761755 1101 52-53 1.0586694626917321 1101 54-55 1.0682451428785242 1101 56-57 0.9699689138342507 1101 58-59 0.8525997110301162 1101 60-61 0.8865140078012814 1101 62-63 0.8640135691134745 1101 64-65 0.8983059552700752 1101 66-67 0.9373750045458422 1101 68-69 0.9051352193713171 1101 70-71 0.8825369686469244 1101 72-73 0.8015185579285102 1101 74-75 0.79675680399383 1101 76-77 0.8585280556526982 1101 78-79 0.8821049152038896 1101 80-81 0.8972629765624447 1101 82-83 0.8583603905446608 1101 84-85 0.858430675279056 1101 86-87 0.8756517197240079 1101 88-89 0.8378965081339231 1101 90-91 0.909739631439848 1101 92-93 0.9787124775145521 1101 94-95 0.9447359267642419 1101 96-97 0.9179501628439084 1101 98-99 0.9197531145975653 1101 100 1.0430717522708761 1102 1 0.7143774359821009 1102 2 0.884319871916265 1102 3 0.8645455685337424 1102 4 0.9649918301973059 1102 5 0.8730279240213079 1102 6 0.6137629077428741 1102 7 0.6986339658234328 1102 8 0.7194773964102481 1102 9 0.881925820354418 1102 10-11 0.8627601387081683 1102 12-13 0.8286570157454989 1102 14-15 0.9867779792390721 1102 16-17 1.210180457436877 1102 18-19 1.2153575039929905 1102 20-21 0.9712388531416281 1102 22-23 1.270442479741142 1102 24-25 1.0845478124058303 1102 26-27 0.9811473661661481 1102 28-29 1.1245342571527601 1102 30-31 1.1444803925366358 1102 32-33 0.9595799968984977 1102 34-35 0.8735519894495525 1102 36-37 0.8325514523955846 1102 38-39 0.9075911819906182 1102 40-41 0.8348544077033182 1102 42-43 0.8787568802010455 1102 44-45 0.9127159290269802 1102 46-47 0.9580598475250355 1102 48-49 0.8898040184655542 1102 50-51 0.9357165484376679 1102 52-53 0.8342544913972745 1102 54-55 0.8999963497473971 1102 56-57 0.8355930129513993 1102 58-59 0.7531461690137533 1102 60-61 0.7953026328533497 1102 62-63 0.7791146054817091 1102 64-65 0.7131710656645396 1102 66-67 0.7484811844654686 1102 68-69 0.8385847946203029 1102 70-71 0.7647682102516242 1102 72-73 0.7211770081985946 1102 74-75 0.7368442615165094 1102 76-77 0.750171196495117 1102 78-79 0.7317040251057563 1102 80-81 0.8018566253586528 1102 82-83 0.7488389143844358 1102 84-85 0.7139303147041609 1102 86-87 0.6699959937975954 1102 88-89 0.7143267589929323 1102 90-91 0.712019990471326 1102 92-93 0.8020563166511359 1102 94-95 0.7093125698489011 1102 96-97 0.7237201225641883 1102 98-99 0.7870604066076474 1102 100 0.8090281745991419 1103 1 0.7234341308483323 1103 2 0.8922586826949122 1103 3 0.8467065789844455 1103 4 0.8855374477278843 1103 5 0.8561407780727279 1103 6 0.6618277076372578 1103 7 0.6762013860147746 1103 8 0.693342205137327 1103 9 0.7716687164483957 1103 10-11 0.8976542236278533 1103 12-13 0.7770104053270117 1103 14-15 0.9630776528952225 1103 16-17 1.2369789851719162 1103 18-19 1.310378734887209 1103 20-21 1.0149599784739536 1103 22-23 1.2270935303864903 1103 24-25 1.083329300176942 1103 26-27 0.9948814675389386 1103 28-29 1.164031322227956 1103 30-31 1.17539986119057 1103 32-33 1.0206315022329946 1103 34-35 0.9914873897943295 1103 36-37 0.9556817206506238 1103 38-39 1.0288739034201413 1103 40-41 0.9931509499396611 1103 42-43 0.989045544181856 1103 44-45 1.0411586526357937 1103 46-47 0.9848014388804103 1103 48-49 0.9679227361164706 1103 50-51 1.0476979291433253 1103 52-53 1.0240084460471195 1103 54-55 0.9811007227031041 1103 56-57 0.8688819194480217 1103 58-59 0.8370317348654126 1103 60-61 0.8585139101156756 1103 62-63 0.8655997648171976 1103 64-65 0.875263145433081 1103 66-67 0.9344738887077995 1103 68-69 0.8965489699111728 1103 70-71 0.7568963817148386 1103 72-73 0.731713112894667 1103 74-75 0.7299853477573919 1103 76-77 0.6899629244712671 1103 78-79 0.7478061786850745 1103 80-81 0.7883346221344212 1103 82-83 0.7222358719047861 1103 84-85 0.6851285182239231 1103 86-87 0.7229502983471043 1103 88-89 0.7308625650991516 1103 90-91 0.7334942554635759 1103 92-93 0.7759315692422852 1103 94-95 0.7444888012475523 1103 96-97 0.8116277479794434 1103 98-99 0.8663805813699703 1103 100 0.8861271966996824 1104 1 0.5782995163309508 1104 2 0.7551530776656321 1104 3 0.7053284316121982 1104 4 0.7998840433355525 1104 5 0.7221838284895767 1104 6 0.4690732665403985 1104 7 0.5393241833128783 1104 8 0.5495284849566602 1104 9 0.710833756548304 1104 10-11 0.6750651805910479 1104 12-13 0.607616417349206 1104 14-15 0.8152773523110852 1104 16-17 0.8941137494722611 1104 18-19 0.8942438686821461 1104 20-21 0.718221197875863 1104 22-23 0.9786732551925681 1104 24-25 0.8784999986454523 1104 26-27 0.758036766961169 1104 28-29 0.9199760352885562 1104 30-31 0.9692663851516841 1104 32-33 0.8061525222994845 1104 34-35 0.6764033537343579 1104 36-37 0.6804845136021314 1104 38-39 0.7719353689902491 1104 40-41 0.6842775515136026 1104 42-43 0.6169206210729001 1104 44-45 0.6700649618619963 1104 46-47 0.6526623979481769 1104 48-49 0.6620248274499758 1104 50-51 0.7601928064352208 1104 52-53 0.7634885714697646 1104 54-55 0.6598785131946272 1104 56-57 0.6020505596804995 1104 58-59 0.5966162448810692 1104 60-61 0.6294678136574987 1104 62-63 0.5890523759106472 1104 64-65 0.590179629885597 1104 66-67 0.7046704201472096 1104 68-69 0.6683609887866098 1104 70-71 0.5072782462028158 1104 72-73 0.49882258935731016 1104 74-75 0.47466784390159944 1104 76-77 0.5341380725565159 1104 78-79 0.5161183283284885 1104 80-81 0.5046736204853346 1104 82-83 0.5305000537210489 1104 84-85 0.5024238699472257 1104 86-87 0.5474946767384843 1104 88-89 0.5049868862942546 1104 90-91 0.5176014553865258 1104 92-93 0.5133072009061728 1104 94-95 0.4814939259447861 1104 96-97 0.5548149461096727 1104 98-99 0.5889932644649711 1104 100 0.6511838411723758 1105 1 0.5556054070012593 1105 2 0.6932248603772173 1105 3 0.6182567385869575 1105 4 0.685424678245866 1105 5 0.6110783278692864 1105 6 0.3704468288045746 1105 7 0.5031725170473678 1105 8 0.4814611545023624 1105 9 0.5924030469657566 1105 10-11 0.6305551192149892 1105 12-13 0.5938225705823399 1105 14-15 0.7693406640237868 1105 16-17 0.8382133049556586 1105 18-19 0.8752999717156413 1105 20-21 0.7065249614773279 1105 22-23 0.9203411859177564 1105 24-25 0.7919912701481309 1105 26-27 0.6378256842665806 1105 28-29 0.9066623813291272 1105 30-31 0.8886828104246121 1105 32-33 0.7386690904320758 1105 34-35 0.6601527436783883 1105 36-37 0.5906470116197795 1105 38-39 0.7190941838361411 1105 40-41 0.6072757182008317 1105 42-43 0.6402461755218454 1105 44-45 0.7301254698507833 1105 46-47 0.6609556067628546 1105 48-49 0.7222011927534098 1105 50-51 0.7803516871126845 1105 52-53 0.7205585017412801 1105 54-55 0.6531011489630032 1105 56-57 0.6479431315781241 1105 58-59 0.6263368962337879 1105 60-61 0.6723675011517969 1105 62-63 0.615336391073491 1105 64-65 0.5663254191280203 1105 66-67 0.6437171241278605 1105 68-69 0.673692890887807 1105 70-71 0.6126623231516959 1105 72-73 0.5358345693717936 1105 74-75 0.5872691652224589 1105 76-77 0.6232762669332104 1105 78-79 0.6333567950272609 1105 80-81 0.6908747392199217 1105 82-83 0.661679014277226 1105 84-85 0.6235684201270111 1105 86-87 0.6488754715094736 1105 88-89 0.5874726279721969 1105 90-91 0.6255686878922404 1105 92-93 0.6686284091130084 1105 94-95 0.5873865470191184 1105 96-97 0.5666403188901086 1105 98-99 0.5887035498585469 1105 100 0.6735590083629646 1106 1 0.46890790673959515 1106 2 0.5486349901301644 1106 3 0.554958460681604 1106 4 0.6736874956062806 1106 5 0.5662828225652703 1106 6 0.27189538985656014 1106 7 0.25296930350970115 1106 8 0.3129025700898467 1106 9 0.5169937222062373 1106 10-11 0.41742122706187956 1106 12-13 0.31268383934185806 1106 14-15 0.5023479393084287 1106 16-17 0.6509003979294619 1106 18-19 0.6711839746388293 1106 20-21 0.4850232906151035 1106 22-23 0.7601595765617049 1106 24-25 0.573838704470873 1106 26-27 0.38808426371084437 1106 28-29 0.614052549359748 1106 30-31 0.6040286397621344 1106 32-33 0.5174475464315833 1106 34-35 0.4020172979547425 1106 36-37 0.3757418139263464 1106 38-39 0.4753988329737169 1106 40-41 0.4837066249789075 1106 42-43 0.48207780103133047 1106 44-45 0.49515559526210495 1106 46-47 0.42787775194707933 1106 48-49 0.4517613890588379 1106 50-51 0.5015440003631539 1106 52-53 0.4489622191079974 1106 54-55 0.498754628953364 1106 56-57 0.42988390673949084 1106 58-59 0.42403279523190207 1106 60-61 0.4308360466456804 1106 62-63 0.38803664581508457 1106 64-65 0.4334592778626245 1106 66-67 0.46958631842751686 1106 68-69 0.42676160834642474 1106 70-71 0.40793762805269296 1106 72-73 0.3667501375398601 1106 74-75 0.3983760864226866 1106 76-77 0.4133584390097198 1106 78-79 0.4112800950695856 1106 80-81 0.4454146566055641 1106 82-83 0.3935837713390242 1106 84-85 0.3796901927325571 1106 86-87 0.37423869001289134 1106 88-89 0.380875968372159 1106 90-91 0.3610638186071924 1106 92-93 0.39746978774536856 1106 94-95 0.4010332461432746 1106 96-97 0.5050730481588417 1106 98-99 0.47415516209440867 1106 100 0.4713270172979733 1107 1 0.4543609643776314 1107 2 0.5886225507987639 1107 3 0.528460795556704 1107 4 0.6445208196786467 1107 5 0.5135475402798662 1107 6 0.29684999259439593 1107 7 0.26513562159355786 1107 8 0.3449208830972097 1107 9 0.40455835449768074 1107 10-11 0.4307070108842197 1107 12-13 0.3046037379009334 1107 14-15 0.5167608754722153 1107 16-17 0.620865228479687 1107 18-19 0.7396229867232833 1107 20-21 0.4921646077913806 1107 22-23 0.7384573329627031 1107 24-25 0.4280224576223759 1107 26-27 0.4163141001769759 1107 28-29 0.6030631150122332 1107 30-31 0.6145477095757528 1107 32-33 0.5158328967665682 1107 34-35 0.41805312604451217 1107 36-37 0.39886552341054227 1107 38-39 0.4552331690846003 1107 40-41 0.2430380683340161 1107 42-43 0.4087846528877108 1107 44-45 0.33073897323330215 1107 46-47 0.31106602148716433 1107 48-49 0.32724712679860346 1107 50-51 0.36201039379423605 1107 52-53 0.3763925377371464 1107 54-55 0.36266471310962345 1107 56-57 0.234629189387924 1107 58-59 0.3098403441798254 1107 60-61 0.3458690786641796 1107 62-63 0.27279427888963426 1107 64-65 0.34144545457226627 1107 66-67 0.37045155208913627 1107 68-69 0.33986604390528186 1107 70-71 0.3137012651976221 1107 72-73 0.33505605100094016 1107 74-75 0.3072941016714239 1107 76-77 0.20948759542449835 1107 78-79 0.19419987064301125 1107 80-81 0.28726178248285095 1107 82-83 0.2945419851132698 1107 84-85 0.2500317409144124 1107 86-87 0.31916473563167713 1107 88-89 0.3508457072351021 1107 90-91 0.386063614865904 1107 92-93 0.4442130389079928 1107 94-95 0.37031295032575073 1107 96-97 0.3185577205169281 1107 98-99 0.34428009624893363 1107 100 0.4226284054799443 1108 1 0.2985020327751755 1108 2 0.40606815588884615 1108 3 0.4213035467872288 1108 4 0.5349199229129624 1108 5 0.45277396022719785 1108 6 0.169938186323094 1108 7 0.19568288662389222 1108 8 0.2106933193502556 1108 9 0.36655663687377427 1108 10-11 0.3189271632781612 1108 12-13 0.23599389623727518 1108 14-15 0.41014726621457953 1108 16-17 0.6107862368574573 1108 18-19 0.6174748297802495 1108 20-21 0.2722802160626827 1108 22-23 0.5144575844814767 1108 24-25 0.3584472055639125 1108 26-27 0.39455601070222457 1108 28-29 0.49896680624691214 1108 30-31 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1 -0.6821430671021567 2216 2 -0.6259446915365103 2216 3 -0.8995375328393962 2216 4 -0.8762805483723426 2216 5 -0.9471106792677588 2216 6 -0.8245608568983727 2216 7 -1.0625414821184194 2216 8 -0.8863388614789898 2216 9 -1.0673660462996963 2216 10-11 -1.0734236086000664 2216 12-13 -1.348219677786929 2216 14-15 -1.7348962031185238 2216 16-17 -1.4289125274255383 2216 18-19 -3.3832387889493205 2216 20-21 -1.6342579261402363 2216 22-23 -1.1184836884098885 2216 24-25 -1.3702022423058509 2216 26-27 -1.3305719971458316 2216 28-29 -1.2356352285154344 2216 30-31 -1.42298427765229 2216 32-33 -1.4074696966773956 2216 34-35 -1.574307973865146 2216 36-37 -1.581263276994754 2216 38-39 -1.6801855515623458 2216 40-41 -1.68371630925688 2216 42-43 -1.6509771342295707 2216 44-45 -1.5812953952676878 2216 46-47 -1.6821873768317772 2216 48-49 -1.7201403309929546 2216 50-51 -1.608093213497142 2216 52-53 -1.5052960312858659 2216 54-55 -1.4245829813026631 2216 56-57 -1.462614666721592 2216 58-59 -1.5777095233065594 2216 60-61 -1.55858077393205 2216 62-63 -1.6307881973818539 2216 64-65 -1.6258184204039736 2216 66-67 -1.5532969368123233 2216 68-69 -1.6165738145098452 2216 70-71 -1.6751022997275768 2216 72-73 -1.6218520189044483 2216 74-75 -1.6865080488534687 2216 76-77 -1.5660541670762953 2216 78-79 -1.7224695941002928 2216 80-81 -1.7264917791669525 2216 82-83 -1.8449553574271675 2216 84-85 -1.7121038493804548 2216 86-87 -1.6245243149897028 2216 88-89 -1.519287120754612 2216 90-91 -1.532945014512162 2216 92-93 -1.63258555103695 2216 94-95 -1.4261192111840622 2216 96-97 -1.5201436405443545 2216 98-99 -1.4288655936761145 2216 100 -1.3843201624699226 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 22273.0 1 3812.0 2 533.0 3 542.0 4 509.0 5 293.0 6 231.0 7 326.0 8 645.0 9 1077.0 10 1801.0 11 3112.0 12 5243.0 13 7623.0 14 10245.0 15 13132.0 16 16204.0 17 19669.0 18 22733.0 19 26141.0 20 30702.0 21 35138.0 22 41041.0 23 47525.0 24 53987.0 25 61540.0 26 70163.0 27 80405.0 28 96093.0 29 113888.0 30 135766.0 31 164325.0 32 204613.0 33 259632.0 34 363598.0 35 495726.0 36 732368.0 37 1125385.0 38 877904.0 39 308929.0 40 328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.66743814016871 21.94377994860135 12.78545136754479 23.60333054368515 2 15.174641591927157 19.99298071298058 41.307988526817006 23.524389168275253 3 18.266732388980316 27.561484808530345 28.94456374419726 25.227219058292082 4 12.071274656058346 17.126599996316553 40.8643387295799 29.937786618045198 5 12.99089222468951 38.829622611600115 35.08985433708803 13.089630826622342 6 28.933782279520027 39.08839903675144 18.629866784263598 13.347951899464938 7 26.291371902617 31.887078847199362 24.440408118823957 17.381141131359676 8 25.740497686370773 35.292204849225364 20.190348418780523 18.77694904562334 9 25.987853482634275 15.175997361375476 22.344930137256384 36.491219018733865 10-11 25.642944119919488 25.96959350350123 27.512788392474995 20.87467398410429 12-13 25.90840662782321 23.863348000524105 27.43432704979013 22.793918321862556 14-15 23.291258228500308 24.934737732849836 25.0891355601314 26.68486847851846 16-17 22.064339927409566 27.42594218749856 26.50475873679012 24.004959148301754 18-19 21.471973079708608 28.30196502647248 28.122278347114747 22.10378354670416 20-21 23.379523325473038 27.414101873276493 26.803285697331653 22.403089103918823 22-23 24.051338185753075 26.928672893105592 26.566196733192015 22.45379218794932 24-25 23.339972277127007 27.067440071677005 27.300488006595703 22.292099644600285 26-27 23.13274881513095 27.37713547266897 26.231197043852127 23.25891866834795 28-29 22.101622081363224 27.948527575309473 26.96577487461204 22.984075468715265 30-31 23.068539301205846 27.394576784237913 26.909358854897846 22.627525059658396 32-33 23.567697548593948 27.728320730423643 25.73335575215532 22.97062596882709 34-35 23.51020937697558 27.4764883624224 26.821404986373583 22.191897274228435 36-37 23.092103068320537 27.074455741694038 26.48358806365085 23.349853126334576 38-39 23.337783792814328 27.008210328303477 27.1452257510853 22.5087801277969 40-41 22.832536139142334 26.07000097314704 27.930554900237688 23.166907987472943 42-43 22.80201886535123 26.44243635063456 27.122775912212347 23.632768871801865 44-45 23.05350985660349 27.46987869639335 26.892220969955144 22.584390477048018 46-47 22.93281677560483 27.102959375176255 26.39177705172815 23.572446797490766 48-49 23.760637284402776 26.551492914373615 26.81000135989189 22.87786844133172 50-51 23.365233751027095 26.49739790304444 26.62892872778252 23.508439618145946 52-53 22.632352498085073 27.191376529741884 26.48938858975206 23.686882382420983 54-55 22.666669246839312 27.07148469676567 26.44050347646012 23.8213425799349 56-57 23.952612886504184 26.357082480209147 26.170539210112807 23.51976542317386 58-59 22.601869725174893 26.870307983949672 27.294629836082763 23.233192454792675 60-61 22.727663895947188 27.620517267718135 26.42959376806485 23.22222506826982 62-63 23.329734305510012 26.72324686053999 25.875050684747368 24.07196814920263 64-65 22.257068658331534 27.00437752978615 26.518046759994014 24.220507051888305 66-67 23.24598821931458 26.49752152637524 26.41623411511576 23.84025613919442 68-69 22.404718870579423 26.650515375368222 26.7726085207892 24.172157233263157 70-71 22.404971986137 26.309506653039065 26.906604976335373 24.378916384488562 72-73 23.136608487981096 26.5584300518235 27.240477746691933 23.064483713503478 74-75 23.326422088903588 26.935166101613078 26.74471909942563 22.993692710057708 76-77 22.90157667885889 26.911697894447734 26.28832160132129 23.898403825372085 78-79 22.789822728691195 26.881939737745448 26.407191125393464 23.921046408169893 80-81 22.359474189571234 27.28063367625491 27.203734080719517 23.15615805345434 82-83 23.408813465371274 26.036752671553298 28.311080025389877 22.243353837685547 84-85 23.66500400239176 26.772715842348425 26.602788119452715 22.9594920358071 86-87 22.132125514691243 27.483675832282522 27.202826755570165 23.18137189745607 88-89 23.27519242794964 26.00042282384975 27.04757454170371 23.6768102064969 90-91 22.87615595186043 26.138640269022872 27.23502561244388 23.750178166672814 92-93 23.15878597227811 26.441435037721277 27.184684029284377 23.215094960716232 94-95 21.94795473159151 26.413849995454104 27.86276270156135 23.775432571393036 96-97 22.389338992514404 26.43352508751729 27.24804867142847 23.929087248539833 98-99 21.694092894843582 27.26986298361321 27.108101413458442 23.92794270808477 100 22.62191072967132 26.35843755148668 26.23150579903343 24.788145919808564 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18790.0 1 10991.0 2 2877.0 3 1997.0 4 1056.5 5 553.5 6 403.0 7 387.5 8 367.0 9 341.0 10 330.5 11 308.5 12 290.5 13 296.0 14 319.0 15 423.5 16 515.5 17 573.5 18 715.0 19 863.0 20 1121.0 21 1452.5 22 2000.0 23 2873.5 24 3982.0 25 5541.5 26 8031.5 27 10673.5 28 15347.5 29 26266.5 30 41582.0 31 56486.0 32 75837.5 33 94860.0 34 110644.0 35 126533.0 36 141209.5 37 170553.5 38 182489.0 39 169080.5 40 155696.0 41 141448.0 42 138889.5 43 148916.0 44 164434.5 45 198943.0 46 234727.0 47 257841.0 48 268561.0 49 258300.5 50 244115.0 51 250964.5 52 254784.5 53 242281.0 54 228942.5 55 197428.5 56 171420.5 57 139744.0 58 107430.0 59 101648.5 60 82956.5 61 55508.5 62 40772.5 63 34283.5 64 24961.0 65 11023.0 66 5240.5 67 3500.0 68 2518.5 69 1875.0 70 1458.0 71 1171.0 72 933.5 73 797.5 74 640.0 75 464.0 76 312.0 77 200.5 78 143.0 79 85.5 80 49.5 81 35.5 82 28.0 83 23.5 84 20.0 85 20.0 86 17.0 87 13.0 88 10.5 89 6.5 90 6.5 91 4.0 92 3.5 93 5.5 94 4.5 95 5.0 96 3.5 97 1.0 98 1.0 99 2.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.995013931661534 2 0.4481778853204282 3 0.48207215134183895 4 0.46744390673119224 5 0.43420956151928436 6 0.485536735591729 7 0.4999083443320135 8 0.5016131397565625 9 0.5156547880920956 10-11 0.5296322774600382 12-13 0.5279916410030796 14-15 0.5248936794251357 16-17 0.5291281712861123 18-19 0.5281199589382607 20-21 0.5274233758615633 22-23 0.49206261915236843 24-25 0.5248936794251357 26-27 0.5058659627511365 28-29 0.5122543628097962 30-31 0.5142066285379088 32-33 0.517167106613873 34-35 0.5277808329667106 36-37 0.5460661387300191 38-39 0.5346916703328933 40-41 0.540841765654788 42-43 0.5385687050887227 44-45 0.5486233318668426 46-47 0.5462311189323948 48-49 0.5187344185364423 50-51 0.5333534975802904 52-53 0.5270842498900131 54-55 0.5353882534095908 56-57 0.5238488048100894 58-59 0.5296689397272327 60-61 0.5237388180085056 62-63 0.5182486434961139 64-65 0.5288898665493474 66-67 0.5274875348291538 68-69 0.5468910397418977 70-71 0.5308329667106614 72-73 0.5350216307376447 74-75 0.5319420002932981 76-77 0.520265068191817 78-79 0.5501723126558146 80-81 0.52506782519431 82-83 0.5247928581903505 84-85 0.5328768881067605 86-87 0.8411973896465758 88-89 1.1093727086083003 90-91 1.0993364129637777 92-93 1.0992997506965831 94-95 1.1039650241970964 96-97 1.0968892066285378 98-99 1.0926180525003666 100 1.0886310309429537 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5455200.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.483076699959415 #Duplication Level Percentage of deduplicated Percentage of total 1 72.74589665793869 16.355515741677568 2 12.659010934623726 5.692270275775404 3 5.126049000607499 3.4574805854522612 4 2.7089861031133218 2.4362536934168393 5 1.6036552369148556 1.802755184592414 6 1.0256530116787201 1.383590119747022 7 0.7032853652024015 1.1068413165463193 8 0.47324672623243563 0.8512033955110836 9 0.3764535812494902 0.7617451267086023 >10 1.936225203582366 8.028352981691212 >50 0.22828901466306284 3.646293224632904 >100 0.29108591345324103 14.685476643779625 >500 0.06304146361750008 9.809293087066218 >1k 0.0552021106282302 22.621235784592127 >5k 0.0031847371516286658 4.787410908942737 >10k+ 7.349393426835383E-4 2.5742819298676425 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 21976 0.40284499193430123 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 19104 0.3501979762428509 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 17800 0.3262941780319695 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 16812 0.30818301803783543 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 15746 0.28864202962311186 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 13978 0.2562325854230826 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 13386 0.24538055433347997 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 11408 0.2091215720780173 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 10004 0.1833846605074058 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 9860 0.18074497726939434 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 9417 0.1726242850857897 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 8716 0.15977416043408124 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 8692 0.159334213227746 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 8613 0.15788605367355918 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 8384 0.15368822407977709 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 8182 0.14998533509312215 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 7739 0.1418646429095175 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 7366 0.135027130077724 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 7277 0.13339565918756416 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 7272 0.13330400351957766 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 7182 0.1316542014958205 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 7154 0.13114092975509606 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 7137 0.13082930048394192 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 7075 0.12969277020090922 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 6898 0.1264481595541868 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 6795 0.12456005279366476 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 6698 0.12278193283472649 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 6639 0.1217003959524857 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 6587 0.120747177005426 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 6540 0.11988561372635283 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 6451 0.118254142836193 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 6119 0.11216820648188884 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 6052 0.11094002053086963 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 6042 0.11075670919489661 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 5975 0.10952852324387741 No Hit GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT 5863 0.10747543628097961 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 5685 0.10421249450065992 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 5585 0.10237938114092975 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 5573 0.10215940753776212 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.8331133597301656E-5 0.0 0.0 0.0 0.0 6 3.666226719460331E-5 0.0 0.0 0.0 0.0 7 3.666226719460331E-5 0.0 0.0 0.0 0.0 8 3.666226719460331E-5 0.0 0.0 0.0 1.8331133597301656E-5 9 3.666226719460331E-5 0.0 0.0 0.0 1.8331133597301656E-5 10-11 1.649802023757149E-4 0.0 0.0 0.0 1.8331133597301656E-5 12-13 1.8331133597301657E-4 0.0 0.0 0.0 1.8331133597301656E-5 14-15 2.0164246957031824E-4 9.165566798650828E-6 0.0 0.0 1.8331133597301656E-5 16-17 2.6580143716087404E-4 1.8331133597301656E-5 0.0 9.165566798650828E-6 1.8331133597301656E-5 18-19 2.7496700395952486E-4 1.8331133597301656E-5 0.0 1.8331133597301656E-5 1.8331133597301656E-5 20-21 2.7496700395952486E-4 1.8331133597301656E-5 0.0 2.7496700395952484E-5 1.8331133597301656E-5 22-23 2.7496700395952486E-4 1.8331133597301656E-5 0.0 3.666226719460331E-5 1.8331133597301656E-5 24-25 3.849538055433348E-4 1.8331133597301656E-5 0.0 9.165566798650829E-5 1.8331133597301656E-5 26-27 4.399472063352398E-4 1.8331133597301656E-5 0.0 2.4747030356357235E-4 1.8331133597301656E-5 28-29 4.5827833993254143E-4 1.8331133597301656E-5 0.0 9.990467810529404E-4 1.8331133597301656E-5 30-31 5.957618419123039E-4 1.8331133597301656E-5 0.0 0.0043719753629564454 1.8331133597301656E-5 32-33 6.41589675905558E-4 1.8331133597301656E-5 0.0 0.01404164833553307 1.8331133597301656E-5 34-35 6.599208095028596E-4 1.8331133597301656E-5 0.0 0.029861416630004398 1.8331133597301656E-5 36-37 8.157354450799237E-4 1.8331133597301656E-5 0.0 0.0575780906291245 1.8331133597301656E-5 38-39 9.715500806569878E-4 1.8331133597301656E-5 0.0 0.1040108520310896 1.8331133597301656E-5 40-41 9.898812142542894E-4 1.8331133597301656E-5 0.0 0.15643789411937234 1.8331133597301656E-5 42-43 9.898812142542894E-4 1.8331133597301656E-5 0.0 0.21401598474849684 1.8331133597301656E-5 44-45 9.898812142542894E-4 1.8331133597301656E-5 0.0 0.28386676932101484 1.8331133597301656E-5 46-47 9.898812142542894E-4 1.8331133597301656E-5 0.0 0.372506965830767 1.8331133597301656E-5 48-49 0.0010357090482475437 1.8331133597301656E-5 0.0 0.47921249450065995 1.8331133597301656E-5 50-51 0.0011640269834286552 1.8331133597301656E-5 0.0 0.5949919343012172 1.8331133597301656E-5 52-53 0.0012098548174219095 1.8331133597301656E-5 0.0 0.7104505792638216 1.8331133597301656E-5 54-55 0.001228185951019211 1.8331133597301656E-5 0.0 0.8394376008212348 1.8331133597301656E-5 56-57 0.001228185951019211 1.8331133597301656E-5 0.0 0.999816688664027 1.8331133597301656E-5 58-59 0.001228185951019211 1.8331133597301656E-5 0.0 1.191725326294178 1.8331133597301656E-5 60-61 0.001228185951019211 1.8331133597301656E-5 0.0 1.3957600087989441 1.8331133597301656E-5 62-63 0.001228185951019211 1.8331133597301656E-5 0.0 1.6137813462384514 1.8331133597301656E-5 64-65 0.001228185951019211 1.8331133597301656E-5 0.0 1.8473016571344774 1.8331133597301656E-5 66-67 0.001228185951019211 1.8331133597301656E-5 0.0 2.116595175245637 1.8331133597301656E-5 68-69 0.001228185951019211 1.8331133597301656E-5 0.0 2.4181973163220416 1.8331133597301656E-5 70-71 0.001228185951019211 1.8331133597301656E-5 0.0 2.7540328493914066 1.8331133597301656E-5 72-73 0.001228185951019211 1.8331133597301656E-5 0.0 3.109345211907905 1.8331133597301656E-5 74-75 0.001228185951019211 1.8331133597301656E-5 0.0 3.464492594222027 1.8331133597301656E-5 76-77 0.001228185951019211 1.8331133597301656E-5 0.0 3.8726352837659483 1.8331133597301656E-5 78-79 0.001228185951019211 1.8331133597301656E-5 0.0 4.311464290951752 1.8331133597301656E-5 80-81 0.001228185951019211 1.8331133597301656E-5 0.0 4.789576917436574 1.8331133597301656E-5 82-83 0.001228185951019211 1.8331133597301656E-5 0.0 5.294398005572664 1.8331133597301656E-5 84-85 0.001228185951019211 1.8331133597301656E-5 0.0 5.792060786039009 1.8331133597301656E-5 86-87 0.001228185951019211 1.8331133597301656E-5 0.0 6.328099794691304 1.8331133597301656E-5 88 0.001228185951019211 3.666226719460331E-5 0.0 6.756140929755096 1.8331133597301656E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5835 0.0 41.35781 94 TATTCTC 2730 0.0 35.100975 5 GTACTAG 7225 0.0 33.04484 1 TACCCCG 385 0.0 32.94236 5 CAACGCA 10850 0.0 31.863958 5 GTATCAA 12440 0.0 31.101725 1 TAGGCAT 8040 0.0 30.965029 5 ATCAACG 11515 0.0 30.071798 3 GCATCGT 7090 0.0 29.860262 8 CGTTAAC 1505 0.0 29.47015 1 AGGGCTA 6685 0.0 29.30126 5 TAGTACT 9300 0.0 28.135586 4 CTGTGCG 9035 0.0 27.904451 9 CTAGGCA 9255 0.0 27.764803 4 GCTATTG 6920 0.0 27.60906 8 GGTATCA 8280 0.0 27.124866 1 CTATTGA 7195 0.0 26.753489 9 GTACTGT 9830 0.0 26.453035 6 ACTGTGC 9945 0.0 26.385803 8 AACGCAG 14305 0.0 26.380768 6 >>END_MODULE