##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146432_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5455200
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84396447426309	31.0	31.0	34.0	30.0	34.0
2	31.843829373808475	31.0	31.0	34.0	30.0	34.0
3	32.00286992227599	33.0	31.0	34.0	30.0	34.0
4	35.751533949259425	37.0	35.0	37.0	35.0	37.0
5	35.65302463704356	37.0	35.0	37.0	33.0	37.0
6	35.68261200322628	37.0	35.0	37.0	33.0	37.0
7	35.61463172752603	37.0	35.0	37.0	33.0	37.0
8	35.59863341399032	37.0	35.0	37.0	33.0	37.0
9	37.193271007479105	39.0	37.0	39.0	33.0	39.0
10-11	37.13549237424842	39.0	37.0	39.0	33.0	39.0
12-13	37.05938324901012	39.0	37.0	39.0	33.0	39.0
14-15	38.25533738451386	40.0	38.0	41.0	33.0	41.0
16-17	38.26463319401672	40.0	37.5	41.0	33.0	41.0
18-19	38.31587375348292	40.0	38.0	41.0	33.0	41.0
20-21	38.32761997726939	40.0	38.0	41.0	33.0	41.0
22-23	38.29248762648483	40.0	38.0	41.0	33.0	41.0
24-25	38.21293783912597	40.0	37.0	41.0	33.0	41.0
26-27	38.06658545974483	40.0	37.0	41.0	33.0	41.0
28-29	38.00711357970377	40.0	37.0	41.0	33.0	41.0
30-31	37.881005920956156	40.0	37.0	41.0	33.0	41.0
32-33	37.756456775186976	40.0	36.5	41.0	33.0	41.0
34-35	37.66086412963777	40.0	36.0	41.0	32.0	41.0
36-37	37.58014536588943	40.0	36.0	41.0	32.0	41.0
38-39	37.458989312949114	40.0	36.0	41.0	32.0	41.0
40-41	37.32825432614753	40.0	36.0	41.0	31.0	41.0
42-43	37.193486764921545	39.0	35.5	41.0	31.0	41.0
44-45	36.97886612773134	39.0	35.0	41.0	31.0	41.0
46-47	36.72137446839713	39.0	35.0	41.0	30.0	41.0
48-49	36.447894577650686	39.0	35.0	41.0	30.0	41.0
50-51	36.23159810822702	38.5	35.0	41.0	29.0	41.0
52-53	36.02699231925502	38.0	35.0	40.5	29.0	41.0
54-55	35.81315927921983	38.0	34.5	40.0	29.0	41.0
56-57	35.5329260155448	37.5	34.0	40.0	28.0	41.0
58-59	35.24097778266608	37.0	34.0	40.0	28.0	41.0
60-61	34.9769585899692	36.5	34.0	40.0	28.0	41.0
62-63	34.70138867502566	36.0	34.0	39.0	27.5	41.0
64-65	34.422366457691744	35.0	33.0	39.0	27.0	41.0
66-67	34.20288385393753	35.0	33.0	39.0	26.5	41.0
68-69	34.02133249010119	35.0	33.0	38.0	26.0	41.0
70-71	33.83941386200323	35.0	33.0	37.5	26.0	40.0
72-73	33.6556899838686	35.0	33.0	37.0	26.0	40.0
74-75	33.483136640269834	35.0	33.0	36.5	26.5	39.0
76-77	33.33949974336413	35.0	33.0	36.0	27.0	39.0
78-79	33.23736682431442	35.0	33.0	36.0	27.0	39.0
80-81	33.105474409737496	35.0	33.0	36.0	27.0	38.0
82-83	32.92410800703915	35.0	33.0	35.0	27.0	37.0
84-85	32.791152111746584	35.0	33.0	35.0	27.0	37.0
86-87	32.66491072737938	35.0	33.0	35.0	26.0	36.5
88-89	32.565692641882976	35.0	33.0	35.0	26.0	36.0
90-91	32.47710963851004	35.0	33.0	35.0	26.0	36.0
92-93	32.39835918023171	35.0	33.0	35.0	26.0	36.0
94-95	32.364481778853204	35.0	33.0	35.0	26.0	35.0
96-97	32.31237039888546	35.0	33.0	35.0	26.0	35.0
98-99	32.28009917143276	35.0	33.0	35.0	26.0	35.0
100	31.777901635137116	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.477849830495817
1101	2	0.5698348510324145
1101	3	0.47855314731244647
1101	4	0.34791462248686855
1101	5	0.3819362048920567
1101	6	0.39589372610160467
1101	7	0.4108705918638407
1101	8	0.39776291351918047
1101	9	0.5176805127953727
1101	10-11	0.543766148989441
1101	12-13	0.5861995411848824
1101	14-15	0.7643919314957088
1101	16-17	0.7005876169250911
1101	18-19	0.7012412878883794
1101	20-21	0.729351319682884
1101	22-23	0.7170930835514895
1101	24-25	0.7438762278592534
1101	26-27	0.7617927862507798
1101	28-29	0.7344653601303079
1101	30-31	0.772575714211527
1101	32-33	0.761351332241432
1101	34-35	0.7912565573184054
1101	36-37	0.8060622228328356
1101	38-39	0.7975327940680756
1101	40-41	0.8189064850442307
1101	42-43	0.8388504397066754
1101	44-45	0.8481549539022808
1101	46-47	0.8526897270683094
1101	48-49	0.8099154918151328
1101	50-51	0.7216227744969714
1101	52-53	0.7930473783963947
1101	54-55	0.7275501800100628
1101	56-57	0.7063003247074491
1101	58-59	0.777294661864552
1101	60-61	0.7598681839108181
1101	62-63	0.7442154381811861
1101	64-65	0.7732905735286408
1101	66-67	0.7018519362196471
1101	68-69	0.6869428917349438
1101	70-71	0.6745222518001057
1101	72-73	0.5757149366378371
1101	74-75	0.5954343160379878
1101	76-77	0.6092427460138126
1101	78-79	0.5674901847868057
1101	80-81	0.5714969262342393
1101	82-83	0.5447133000940951
1101	84-85	0.584084277389131
1101	86-87	0.5855408807525535
1101	88-89	0.5582568066860105
1101	90-91	0.5365024115432746
1101	92-93	0.5357856699574768
1101	94-95	0.5607183013809376
1101	96-97	0.5856306959889039
1101	98-99	0.5848078006330653
1101	100	0.678654880012413
1102	1	0.42232521397808753
1102	2	0.5224100713320006
1102	3	0.40274013888188165
1102	4	0.3622005733120801
1102	5	0.31714913035639114
1102	6	0.3575424980624433
1102	7	0.34238797204697136
1102	8	0.34900681560483093
1102	9	0.4040107621464415
1102	10-11	0.4931190730167927
1102	12-13	0.5122966852956878
1102	14-15	0.6541419875999281
1102	16-17	0.6751323301342538
1102	18-19	0.6645260483285682
1102	20-21	0.5925391876311679
1102	22-23	0.5830248466370733
1102	24-25	0.5849434024156466
1102	26-27	0.591778743196457
1102	28-29	0.6208316152280986
1102	30-31	0.6665989302675897
1102	32-33	0.7343892518883735
1102	34-35	0.730966926529959
1102	36-37	0.7035651566617958
1102	38-39	0.7469246362347235
1102	40-41	0.6911495950182385
1102	42-43	0.7539491032880719
1102	44-45	0.6768404388629463
1102	46-47	0.7136062023104657
1102	48-49	0.7018126391192183
1102	50-51	0.6690447822501469
1102	52-53	0.6694944309516302
1102	54-55	0.7827830915836174
1102	56-57	0.7619542322364694
1102	58-59	0.7285979353642347
1102	60-61	0.6839223585520742
1102	62-63	0.696668071885874
1102	64-65	0.5955888859080289
1102	66-67	0.6155085293589977
1102	68-69	0.6113725284782845
1102	70-71	0.6339704524589322
1102	72-73	0.6735052068221137
1102	74-75	0.6135977997207576
1102	76-77	0.6177943806643711
1102	78-79	0.5954229641052109
1102	80-81	0.5946938299913498
1102	82-83	0.600609246664547
1102	84-85	0.5603599570779849
1102	86-87	0.5868374276602566
1102	88-89	0.5417977896672852
1102	90-91	0.5669544104350877
1102	92-93	0.5878469638462747
1102	94-95	0.5457197309665318
1102	96-97	0.5321604841470915
1102	98-99	0.5603373090832875
1102	100	0.6445938279852506
1103	1	0.305412000380624
1103	2	-0.1754679123775169
1103	3	0.2011647177325102
1103	4	0.22305554974181518
1103	5	0.28218165831277986
1103	6	0.30894601188245474
1103	7	0.3538281380072661
1103	8	0.36196621369391835
1103	9	0.4234440882528432
1103	10-11	0.45634503360022194
1103	12-13	0.4515242677220215
1103	14-15	0.5901760766670847
1103	16-17	0.5933535577383182
1103	18-19	0.5944615841119116
1103	20-21	0.567539974790428
1103	22-23	0.4826205150558778
1103	24-25	0.5314334503934433
1103	26-27	0.5389699157034968
1103	28-29	0.5675770698550338
1103	30-31	0.6040131571778389
1103	32-33	0.6213742971594627
1103	34-35	0.5694577942147276
1103	36-37	0.572184773826443
1103	38-39	0.6402189473020172
1103	40-41	0.6640224503998624
1103	42-43	0.6739139107828223
1103	44-45	0.6511633772323933
1103	46-47	0.6160550724833698
1103	48-49	0.6261303857752765
1103	50-51	0.6487098271132155
1103	52-53	0.6517573446237179
1103	54-55	0.6542345068458673
1103	56-57	0.6327239292461897
1103	58-59	0.5837306619147142
1103	60-61	0.606969550548591
1103	62-63	0.5997864549204053
1103	64-65	0.5599319861681167
1103	66-67	0.5417068496259816
1103	68-69	0.5405542413356699
1103	70-71	0.5327909753697213
1103	72-73	0.4599548894495271
1103	74-75	0.4978945265710024
1103	76-77	0.5015003916542895
1103	78-79	0.4720238603228921
1103	80-81	0.4343851229308129
1103	82-83	0.4375463960949162
1103	84-85	0.4004472545149227
1103	86-87	0.4836623249359704
1103	88-89	0.4620046117850265
1103	90-91	0.4385834229794554
1103	92-93	0.4224940031044184
1103	94-95	0.45753585539642216
1103	96-97	0.45162909385695116
1103	98-99	0.5321858771521377
1103	100	0.5362112686987466
1104	1	0.3729983077362071
1104	2	0.4676526025665204
1104	3	0.358314616121298
1104	4	0.2791296703865953
1104	5	0.258255193068031
1104	6	0.28663819860049955
1104	7	0.2550392768187706
1104	8	0.24926343603507206
1104	9	0.3396784110133737
1104	10-11	0.35294031021226857
1104	12-13	0.3820463105913916
1104	14-15	0.48758553385323467
1104	16-17	0.49931337845835344
1104	18-19	0.5036761310422335
1104	20-21	0.5112814491727988
1104	22-23	0.49152182113049037
1104	24-25	0.47071845735526807
1104	26-27	0.5546287452770571
1104	28-29	0.5659311542770453
1104	30-31	0.5785441068477226
1104	32-33	0.5525527532949397
1104	34-35	0.5705524631402739
1104	36-37	0.5939655230044423
1104	38-39	0.6051713891245996
1104	40-41	0.5923254088665573
1104	42-43	0.5672732466500605
1104	44-45	0.5108841204730439
1104	46-47	0.5361033385410963
1104	48-49	0.5153045063282562
1104	50-51	0.49172377470581097
1104	52-53	0.5225233921197798
1104	54-55	0.4691150603573462
1104	56-57	0.4886284479483791
1104	58-59	0.4937238980343466
1104	60-61	0.4882026632775478
1104	62-63	0.48803452366188793
1104	64-65	0.428789531914731
1104	66-67	0.4708059334766972
1104	68-69	0.4956256048221732
1104	70-71	0.47715581979475985
1104	72-73	0.4754724142092712
1104	74-75	0.4808755347088294
1104	76-77	0.4672110471347466
1104	78-79	0.4215679222615947
1104	80-81	0.42167809437359693
1104	82-83	0.44926315728982047
1104	84-85	0.37649595295132343
1104	86-87	0.3508375108842614
1104	88-89	0.36732857823042053
1104	90-91	0.35193901399440364
1104	92-93	0.3072533097550689
1104	94-95	0.36614529612425173
1104	96-97	0.4097234497794986
1104	98-99	0.3654075548531921
1104	100	0.4157967131887297
1105	1	0.10900988018181579
1105	2	-0.4774846356086542
1105	3	-0.03218921111644946
1105	4	0.07984423499525661
1105	5	0.1326093243685662
1105	6	0.1448923676366789
1105	7	0.20202123894135582
1105	8	0.156588126060889
1105	9	0.25979942155704805
1105	10-11	0.26437992846632596
1105	12-13	0.2682503283059816
1105	14-15	0.3210275925213608
1105	16-17	0.36637590940265596
1105	18-19	0.36726612816471516
1105	20-21	0.3532982192966472
1105	22-23	0.35217381613271925
1105	24-25	0.31639290749796345
1105	26-27	0.4074516467517526
1105	28-29	0.38871205097149186
1105	30-31	0.3284922132038375
1105	32-33	0.3426849269493317
1105	34-35	0.3516381278906522
1105	36-37	0.4159887860827425
1105	38-39	0.41728762577906764
1105	40-41	0.4499967457166534
1105	42-43	0.4373556412586197
1105	44-45	0.4222908976579518
1105	46-47	0.4324676155108875
1105	48-49	0.3801852287877594
1105	50-51	0.45382027683240267
1105	52-53	0.44683237595012315
1105	54-55	0.3660245466214107
1105	56-57	0.4464556654255958
1105	58-59	0.4183869473064874
1105	60-61	0.35756230304762937
1105	62-63	0.3546152044624762
1105	64-65	0.42369213610351153
1105	66-67	0.4179742440443448
1105	68-69	0.38829677281415087
1105	70-71	0.3834035197312602
1105	72-73	0.31453524733215943
1105	74-75	0.3669563661504256
1105	76-77	0.3308650283050767
1105	78-79	0.3059392113396555
1105	80-81	0.35128116043483004
1105	82-83	0.25207612697315085
1105	84-85	0.27651376659930094
1105	86-87	0.32070239640891884
1105	88-89	0.3005608167681686
1105	90-91	0.25980735107128083
1105	92-93	0.275261713754098
1105	94-95	0.3100988701685665
1105	96-97	0.28470675075809737
1105	98-99	0.3030274960753516
1105	100	0.28471221400763014
1106	1	0.26296010161970074
1106	2	0.2952751936553817
1106	3	0.25364993185743856
1106	4	0.18723531153038664
1106	5	0.2295214900550775
1106	6	0.21483177177709223
1106	7	0.23488348880227505
1106	8	0.22212036508082633
1106	9	0.23999368931653464
1106	10-11	0.24095275533571225
1106	12-13	0.252426030128845
1106	14-15	0.33536524758607555
1106	16-17	0.3127263737409791
1106	18-19	0.3160733528708448
1106	20-21	0.33914833806239386
1106	22-23	0.28634792702924017
1106	24-25	0.29367500787356704
1106	26-27	0.3021320231085838
1106	28-29	0.27769865008475136
1106	30-31	0.27886874380263293
1106	32-33	0.3237521590239396
1106	34-35	0.29250734660723765
1106	36-37	0.2551848093985072
1106	38-39	0.24572024972805906
1106	40-41	0.22673031527691734
1106	42-43	0.27892053791316584
1106	44-45	0.3203479056342715
1106	46-47	0.22939979333963834
1106	48-49	0.26247445925424984
1106	50-51	0.2518982903234317
1106	52-53	0.25357542164374536
1106	54-55	0.29767847574411377
1106	56-57	0.2549496344373523
1106	58-59	0.26623282398225
1106	60-61	0.27757842155936174
1106	62-63	0.253741903458085
1106	64-65	0.2805452228408001
1106	66-67	0.2780069017902633
1106	68-69	0.2286193208540368
1106	70-71	0.2568831224763244
1106	72-73	0.25752061374531365
1106	74-75	0.2925826321872833
1106	76-77	0.2663242000497519
1106	78-79	0.268003137696077
1106	80-81	0.23624095591029004
1106	82-83	0.25104009792421067
1106	84-85	0.23481528082588454
1106	86-87	0.23339966936358536
1106	88-89	0.2026697061675975
1106	90-91	0.2246337363462274
1106	92-93	0.21445146518878744
1106	94-95	0.17146643825211072
1106	96-97	0.20823810695652156
1106	98-99	0.29764261036542905
1106	100	0.3313261891749626
1107	1	0.05446331221187606
1107	2	-0.4903363747005578
1107	3	-0.04917258874080588
1107	4	0.028205238576191505
1107	5	0.005686316011868087
1107	6	0.11325118395015465
1107	7	0.13055424585301978
1107	8	0.16965420650787877
1107	9	0.139291226417825
1107	10-11	0.2110735405768409
1107	12-13	0.17183348009554322
1107	14-15	0.24309702552140777
1107	16-17	0.2656878829378613
1107	18-19	0.22398655315741678
1107	20-21	0.17886090356521578
1107	22-23	0.18469843760489368
1107	24-25	0.16867991618628508
1107	26-27	0.21351169610012022
1107	28-29	0.11354744738143552
1107	30-31	0.21752205837695016
1107	32-33	0.21739263419306099
1107	34-35	0.22756601668787368
1107	36-37	0.2723128759826423
1107	38-39	0.2531369880746084
1107	40-41	0.23012075543959298
1107	42-43	0.2187700916456663
1107	44-45	0.22749781698829707
1107	46-47	0.2791943480389918
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2216	86-87	-0.6639679592201517
2216	88-89	-0.6751251298430176
2216	90-91	-0.6552742702288477
2216	92-93	-0.6994289408205994
2216	94-95	-0.6851309325238546
2216	96-97	-0.6617867509032465
2216	98-99	-0.6895239764902215
2216	100	-0.6543493215208933
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	10.0
13	55.0
14	292.0
15	920.0
16	2401.0
17	4856.0
18	8707.0
19	13471.0
20	19812.0
21	28070.0
22	38650.0
23	45584.0
24	46927.0
25	48681.0
26	54475.0
27	67135.0
28	83535.0
29	104893.0
30	129917.0
31	161399.0
32	199731.0
33	254170.0
34	347961.0
35	486636.0
36	721544.0
37	1221634.0
38	976078.0
39	386810.0
40	843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.492958015025756	22.350302016738844	13.946065440657337	24.21067452757806
2	14.712375692289022	20.397931331968568	40.57006612940156	24.319626846340846
3	17.791809950462714	27.231519667070142	29.743323795439956	25.233346587027185
4	12.240290709379734	17.606433364248158	39.47616056199706	30.677115364375044
5	12.593413579583807	38.92050693651854	35.02007867217154	13.466000811726106
6	28.786350637923448	39.524288752016425	18.018972723273208	13.67038788678692
7	25.414675360314092	32.643028055399185	24.401526551596643	17.540770032690084
8	25.961284645842497	34.882937380847636	19.8702155741311	19.285562399178765
9	26.010833699956002	14.672239331280245	22.197371315442147	37.1195556533216
10-11	25.666501686464294	26.046111966564013	27.18321051473823	21.104175832233466
12-13	26.053499413403724	23.91475106320575	27.72804846751723	22.303701055873297
14-15	23.347916507781928	24.734586102816515	25.72599019412293	26.19150719527863
16-17	21.613766499765948	27.16823590369969	27.121792610300986	24.09620498623337
18-19	21.046927702009093	27.321308109693504	29.79965904091509	21.832105147382315
20-21	22.71764471243757	26.54030156192314	28.844461740893728	21.897591984745556
22-23	23.285962909992406	25.93960545992951	28.65720578050661	22.11722584957147
24-25	22.68137031468785	26.130355086005565	29.389896739798427	21.798377859508165
26-27	22.4964329849832	26.46166500296094	28.309867765412083	22.73203424664377
28-29	21.53893532776067	27.030457178471917	29.01849611379968	22.412111379967737
30-31	22.59877731338906	26.476902771667397	28.81407647748937	22.110243437454173
32-33	23.111719093708754	26.8145439213961	27.82755902625018	22.246177958644964
34-35	22.912434256239784	26.609327234293378	28.756875007962623	21.721363501504214
36-37	22.686180158380996	26.09382790731779	28.43245893826074	22.787532996040476
38-39	22.83735701715794	26.019724299750695	29.15097521630738	21.991943466783987
40-41	22.296427927872603	25.279307872644374	29.736254397425128	22.688009802057895
42-43	22.508973832421063	25.581272358964664	28.695219476802404	23.21453433181187
44-45	22.70925905558	26.588502713007774	28.312994940607126	22.3892432908051
46-47	22.75571014811556	26.38528376594809	27.54272987241531	23.316276213521046
48-49	23.802330117305107	26.05146011787704	27.562254636697332	22.58395512812052
50-51	23.126349457980407	26.156082834367467	27.207208462311964	23.510359245340158
52-53	22.330876961431294	26.769321014811553	27.12641149728699	23.773390526470155
54-55	22.494801961254275	26.754127840819258	26.876387728549872	23.874682469376598
56-57	23.72170014087489	26.039719936684207	26.65978943007537	23.578790492365535
58-59	22.326694608867392	26.509253253255455	27.800656604138137	23.36339553373902
60-61	22.56699141213179	27.278298275469687	26.843529255734282	23.31118105666424
62-63	23.169452998973455	26.31752273060566	26.334799824021115	24.178224446399767
64-65	22.125980715647454	26.52727672679279	26.95396135797038	24.392781199589383
66-67	22.958608300337293	26.130939287285525	26.88423889133304	24.02621352104414
68-69	22.32220757241002	25.95832456865163	27.340481647329373	24.378986211608975
70-71	22.230644423013153	25.685163347209496	27.472535630950446	24.611656598826908
72-73	22.97539045314562	25.779238158087697	27.758038202082417	23.487333186684264
74-75	23.079415411531187	26.34028378123724	27.21462578374934	23.365675023482233
76-77	22.70659801850098	26.32342217192032	26.63439022324586	24.33558958633284
78-79	22.432809386984328	26.413528229777604	26.899415884434774	24.25424649880329
80-81	22.010147307729746	26.88320697013754	27.639076355585097	23.467569366547615
82-83	23.064299034068593	25.624482422174854	28.72258470173998	22.588633842016574
84-85	23.423692543726702	26.290072745001652	26.88978384753532	23.39645086373633
86-87	21.787592384315086	26.99850315181414	27.548881305346946	23.66502315852383
88-89	22.800000366650565	25.63084521204777	27.41013623818354	24.159018183118125
90-91	22.3048879145972	25.678192627806578	27.69221560860019	24.32470384899603
92-93	22.721262533233666	26.022799405472025	27.33377667802222	23.922161383272094
94-95	21.421557695993364	25.92817254339941	28.134230729712456	24.516039030894767
96-97	21.828543752432434	25.900905071026564	27.561842670420578	24.708708506120423
98-99	21.124016140100604	26.723373146473623	27.466593927508498	24.68601678591727
100	22.07308139192482	25.803326745011013	26.595484898504797	25.528106964559377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	104.0
1	73.5
2	73.5
3	98.5
4	66.0
5	54.5
6	121.0
7	324.5
8	563.5
9	635.5
10	512.0
11	378.0
12	409.5
13	499.5
14	644.5
15	991.0
16	1423.5
17	1970.0
18	2751.5
19	3761.5
20	4992.5
21	6183.5
22	7467.0
23	8775.0
24	10010.5
25	11522.5
26	13661.0
27	15810.0
28	19734.0
29	29937.0
30	44525.5
31	57774.0
32	76661.5
33	95377.5
34	109113.5
35	123759.0
36	137179.5
37	166836.5
38	180466.5
39	166533.5
40	152305.5
41	138814.5
42	136656.0
43	145029.0
44	159872.5
45	196150.5
46	232007.5
47	256650.0
48	270076.5
49	258456.5
50	242435.5
51	247014.5
52	251375.5
53	241611.5
54	231936.0
55	200652.0
56	173114.5
57	140750.5
58	107407.5
59	104827.5
60	86194.5
61	56546.5
62	40287.5
63	33244.0
64	24095.0
65	9734.5
66	4310.0
67	2943.5
68	2195.5
69	1648.5
70	1258.5
71	1005.0
72	768.5
73	671.0
74	512.5
75	357.5
76	246.5
77	145.5
78	91.5
79	40.0
80	18.0
81	8.5
82	4.0
83	3.0
84	1.5
85	0.0
86	0.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012850124651708462
2	0.005297697609620179
3	0.002144742630884294
4	0.027606687197536294
5	0.002896319108373662
6	0.0
7	5.316028743217481E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009385540401818448
16-17	0.002942146942366916
18-19	0.0
20-21	3.666226719460331E-5
22-23	6.965830766974629E-4
24-25	9.165566798650828E-6
26-27	1.558146355770641E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.491127731338906E-4
36-37	0.0
38-39	0.0
40-41	3.4829153834873146E-4
42-43	6.140929755096055E-4
44-45	0.0
46-47	0.0
48-49	4.399472063352398E-4
50-51	9.165566798650828E-6
52-53	0.0
54-55	3.20794837952779E-4
56-57	9.165566798650829E-5
58-59	1.924769027716674E-4
60-61	8.249010118785745E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.001237351517817862
70-71	7.515764774893679E-4
72-73	0.0
74-75	2.199736031676199E-4
76-77	0.0025296964364276285
78-79	5.407684411203988E-4
80-81	0.00287798797477636
82-83	0.0018789411937234198
84-85	0.005930121718727086
86-87	0.007424109106907171
88-89	0.0076074204428801875
90-91	0.005444346678398593
92-93	0.008294837952779
94-95	0.0015673119225692918
96-97	0.004931074937674146
98-99	0.0016772987241531017
100	0.0851847778266608
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5455200.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.60409874948202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.32755964472334	10.847052514036626
2	15.535906095339383	5.159194379393895
3	6.6279105603992114	3.301514443428096
4	3.5076915919433342	2.3296823030141964
5	2.1627264289217707	1.7955061596965847
6	1.339320230328833	1.3342923216935367
7	0.8958252230237085	1.0412059325753686
8	0.6655233136866953	0.884033185642911
9	0.47047696920982407	0.7030661450505238
>10	2.547244233097424	7.8837864988836825
>50	0.31543291439897053	3.7273937054470596
>100	0.42022313499144376	15.56114779024787
>500	0.09346075123329572	10.760392579667052
>1k	0.08462285513558129	26.17622475788123
>5k	0.0051922639574051195	6.0584163780010964
>10k+	8.83789609771084E-4	2.437090905340296
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	24787	0.4543738084763162	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	19008	0.3484381874175099	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	18404	0.33736618272473967	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	16412	0.3008505645989148	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	16137	0.29580950285965685	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	15413	0.2825377621352104	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	12507	0.2292674879014518	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10193	0.18684924475729578	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9954	0.1824681038275407	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	9848	0.18052500366622673	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	9631	0.17654714767561228	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	9571	0.17544727965977416	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	9096	0.16673999120105587	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	9023	0.16540181844845286	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8954	0.16413697023023904	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	8753	0.16045241237718139	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	8565	0.15700615926088868	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	8413	0.15421982695409883	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	8351	0.15308329667106613	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	8248	0.15119518991054406	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	8187	0.15007699076110867	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7923	0.14523757149142102	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	7788	0.1427628684557853	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7710	0.14133304003519578	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	7425	0.13610866695996482	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	7338	0.13451385833699955	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	7337	0.13449552720340227	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7185	0.1317091948966124	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	7048	0.12919782959378207	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	6989	0.1281162927115413	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6939	0.1271997360316762	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	6667	0.12221366769321015	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	6548	0.12003226279513124	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	6530	0.11970230239037982	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	6506	0.11926235518404459	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	6391	0.11715427482035488	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	6201	0.11367135943686757	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6130	0.11236984895145917	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	6106	0.11192990174512393	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	6053	0.11095835166446692	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	5928	0.10866695996480423	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	5922	0.10855697316322042	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	5882	0.10782372781932834	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	5879	0.10776873441853643	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	5875	0.10769540988414723	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	5778	0.10591728992520898	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	5727	0.10498240211174659	No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG	5478	0.10041794984601848	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.8331133597301656E-5	0.0	0.0	0.0	0.0
7	1.8331133597301656E-5	0.0	0.0	0.0	0.0
8	1.8331133597301656E-5	0.0	0.0	0.0	0.0
9	1.8331133597301656E-5	0.0	0.0	0.0	0.0
10-11	1.0998680158380995E-4	0.0	0.0	0.0	0.0
12-13	1.0998680158380995E-4	0.0	0.0	0.0	0.0
14-15	1.558146355770641E-4	0.0	0.0	0.0	0.0
16-17	2.1080803636896908E-4	0.0	0.0	9.165566798650828E-6	0.0
18-19	2.199736031676199E-4	0.0	0.0	1.8331133597301656E-5	0.0
20-21	2.199736031676199E-4	0.0	0.0	3.666226719460331E-5	0.0
22-23	2.199736031676199E-4	0.0	0.0	5.4993400791904974E-5	0.0
24-25	3.20794837952779E-4	0.0	0.0	1.0998680158380995E-4	0.0
26-27	4.0328493914063647E-4	0.0	0.0	2.841325707581757E-4	0.0
28-29	4.0328493914063647E-4	0.0	0.0	0.0010540401818448452	0.0
30-31	4.216160727379381E-4	0.0	0.0	0.0044452998973456516	0.0
32-33	4.5827833993254143E-4	0.0	0.0	0.014234125238304736	0.0
34-35	4.8577504032849394E-4	0.0	0.0	0.030237204868749083	0.0
36-37	5.957618419123039E-4	0.0	0.0	0.058375494940607134	0.0
38-39	6.96583076697463E-4	0.0	0.0	0.10527570024930341	0.0
40-41	7.149142102947647E-4	1.8331133597301656E-5	0.0	0.1583809942806863	0.0
42-43	7.240797770934155E-4	1.8331133597301656E-5	0.0	0.2166190057193137	0.0
44-45	7.515764774893679E-4	1.8331133597301656E-5	0.0	0.2874688370728846	0.0
46-47	7.699076110866696E-4	1.8331133597301656E-5	0.0	0.3773922129344479	0.0
48-49	7.699076110866696E-4	1.8331133597301656E-5	0.0	0.4856192256929168	0.0
50-51	8.340665786772253E-4	1.8331133597301656E-5	0.0	0.6030393019504326	0.0
52-53	8.432321454758762E-4	1.8331133597301656E-5	0.0	0.7200835899692037	0.0
54-55	8.432321454758762E-4	1.8331133597301656E-5	0.0	0.8499505059392873	0.0
56-57	8.432321454758762E-4	1.8331133597301656E-5	0.0	1.0114386273647162	0.0
58-59	8.432321454758762E-4	1.8331133597301656E-5	0.0	1.2049145769174365	0.0
60-61	8.432321454758762E-4	3.666226719460331E-5	0.0	1.4100674585716382	0.0
62-63	8.432321454758762E-4	3.666226719460331E-5	0.0	1.6303160287432175	0.0
64-65	8.432321454758762E-4	3.666226719460331E-5	0.0	1.8662285525736912	0.0
66-67	8.432321454758762E-4	3.666226719460331E-5	0.0	2.1379601114532925	0.0
68-69	8.432321454758762E-4	3.666226719460331E-5	0.0	2.4417069951605805	0.0
70-71	8.432321454758762E-4	3.666226719460331E-5	0.0	2.7811262648482185	0.0
72-73	8.432321454758762E-4	3.666226719460331E-5	0.0	3.1404439800557267	0.0
74-75	8.432321454758762E-4	3.666226719460331E-5	0.0	3.500091655667987	0.0
76-77	8.432321454758762E-4	3.666226719460331E-5	0.0	3.915869262355184	0.0
78-79	8.432321454758762E-4	3.666226719460331E-5	0.0	4.363112259862151	0.0
80-81	8.432321454758762E-4	3.666226719460331E-5	0.0	4.8502896319108375	0.0
82-83	8.432321454758762E-4	3.666226719460331E-5	0.0	5.365257002493034	0.0
84-85	8.432321454758762E-4	3.666226719460331E-5	0.0	5.8734601847778265	0.0
86-87	8.432321454758762E-4	3.666226719460331E-5	0.0	6.4191597008359	0.0
88	8.615632790731778E-4	3.666226719460331E-5	0.0	6.854909077577357	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5560	0.0	42.468773	94
GGTATCA	11885	0.0	39.643677	1
GTATCAA	20195	0.0	37.487583	1
GTGGTAT	2570	0.0	35.12962	1
TATTCTC	3045	0.0	33.64679	5
CAACGCA	22095	0.0	32.692966	5
TACCCCG	470	0.0	31.998276	5
ATCAACG	22620	0.0	31.944717	3
GTACTAG	6780	0.0	30.099926	1
TATCAAC	25680	0.0	29.403036	2
CTGTGCG	8700	0.0	29.068129	9
AACGCAG	26545	0.0	28.610477	6
TAGTACT	8975	0.0	28.390863	4
ACTGTGC	9515	0.0	27.65959	8
GTACTGT	9375	0.0	27.47108	6
TCCGCAC	275	0.0	27.353003	3
TATCCGC	190	4.456524E-10	27.22353	1
CAGTACG	865	0.0	27.171144	9
ACGCAGA	26760	0.0	26.870255	7
CGCAGAG	26890	0.0	26.705399	8
>>END_MODULE