##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146431_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4734183
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.562576055889686	31.0	31.0	34.0	27.0	34.0
2	30.664223584090433	31.0	31.0	34.0	27.0	34.0
3	30.843339178058812	31.0	31.0	34.0	27.0	34.0
4	34.32400479660377	37.0	35.0	37.0	30.0	37.0
5	34.535676166299446	37.0	35.0	37.0	30.0	37.0
6	34.7391288000485	37.0	35.0	37.0	31.0	37.0
7	34.64012502262798	37.0	35.0	37.0	31.0	37.0
8	34.5805278334192	37.0	35.0	37.0	30.0	37.0
9	35.97441902858424	39.0	35.0	39.0	30.0	39.0
10-11	35.88527165088464	39.0	35.0	39.0	30.0	39.0
12-13	35.856008417925544	39.0	35.0	39.0	30.0	39.0
14-15	36.879781896897526	40.0	36.0	41.0	30.0	41.0
16-17	36.72403400966967	40.0	36.0	41.0	29.5	41.0
18-19	36.68476725551167	40.0	36.0	41.0	28.5	41.0
20-21	37.02078711363714	40.0	36.0	41.0	30.0	41.0
22-23	36.74272297881176	40.0	36.0	41.0	29.5	41.0
24-25	36.85490696071529	40.0	36.0	41.0	30.0	41.0
26-27	36.91787600944028	40.0	36.0	41.0	30.0	41.0
28-29	36.76778221289713	40.0	36.0	41.0	29.5	41.0
30-31	36.725762185365454	40.0	36.0	41.0	30.0	41.0
32-33	36.83146321128693	40.0	35.5	41.0	30.0	41.0
34-35	36.880744681817326	40.0	36.0	41.0	30.0	41.0
36-37	36.81703252704849	40.0	35.0	41.0	30.0	41.0
38-39	36.64251614270086	39.5	35.0	41.0	30.0	41.0
40-41	36.595343589379624	39.0	35.0	41.0	30.0	41.0
42-43	36.438373844019125	39.0	35.0	41.0	29.0	41.0
44-45	36.24135663957223	39.0	35.0	41.0	28.5	41.0
46-47	36.10029724241754	39.0	35.0	41.0	28.5	41.0
48-49	35.961924475669825	38.5	34.5	40.5	28.0	41.0
50-51	35.72189097886584	38.0	34.0	40.0	28.0	41.0
52-53	35.51872498380396	38.0	34.0	40.0	27.5	41.0
54-55	35.31878647276626	37.5	34.0	40.0	27.0	41.0
56-57	35.15114445301333	37.0	34.0	40.0	27.0	41.0
58-59	34.86901245684842	36.5	33.0	40.0	27.0	41.0
60-61	34.61654650865841	36.0	33.0	39.5	26.5	41.0
62-63	34.34306964897639	35.5	33.0	39.0	26.0	41.0
64-65	34.048287951690924	35.0	33.0	39.0	26.0	41.0
66-67	33.766526029940124	35.0	32.5	38.5	26.0	40.5
68-69	33.48434999238517	35.0	32.0	38.0	26.0	40.0
70-71	33.44426313896189	35.0	32.0	37.0	26.0	40.0
72-73	33.25494515104296	35.0	32.0	37.0	26.0	40.0
74-75	33.00153891389496	35.0	32.0	36.0	25.0	39.5
76-77	32.786784646896834	35.0	32.0	36.0	25.0	39.0
78-79	32.58319059487138	35.0	32.0	36.0	25.0	39.0
80-81	32.38591009261788	35.0	32.0	35.5	24.5	38.0
82-83	32.150128649441726	35.0	31.5	35.0	24.0	37.0
84-85	31.98015011249037	35.0	31.0	35.0	24.0	37.0
86-87	31.820828113319656	35.0	31.5	35.0	24.0	36.5
88-89	31.82911148555094	35.0	32.0	35.0	24.0	36.0
90-91	31.751720729849268	35.0	32.0	35.0	24.0	36.0
92-93	31.60695995486444	35.0	32.0	35.0	24.0	36.0
94-95	31.600932198860924	35.0	32.0	35.0	24.0	35.5
96-97	31.511759156754188	35.0	32.0	35.0	24.0	35.0
98-99	31.43508394162203	35.0	31.5	35.0	23.5	35.0
100	30.95262244826615	34.0	31.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7966601008644538
1101	2	0.940137634238507
1101	3	0.8705368429966107
1101	4	0.9746805214275156
1101	5	0.8842890721949885
1101	6	0.579275597164397
1101	7	0.6605617876040739
1101	8	0.7350005269101416
1101	9	0.9044458303755576
1101	10-11	0.9470932844412232
1101	12-13	0.8337489587303537
1101	14-15	1.0442582783094778
1101	16-17	1.1357504819852338
1101	18-19	1.283461452157077
1101	20-21	0.9573497350023814
1101	22-23	1.275581450061786
1101	24-25	1.1237456216958535
1101	26-27	1.0646251261480515
1101	28-29	1.1964294807455857
1101	30-31	1.2513518725823616
1101	32-33	1.0790521832261248
1101	34-35	1.0284392429961713
1101	36-37	0.9933790963252065
1101	38-39	1.0117617368510707
1101	40-41	0.9603616971624191
1101	42-43	0.9742482759949453
1101	44-45	1.0189539294268641
1101	46-47	1.0994533420882533
1101	48-49	1.064814626167994
1101	50-51	1.0259593577600015
1101	52-53	1.0451098235576168
1101	54-55	1.0431777629327001
1101	56-57	0.9075628317751736
1101	58-59	0.834958819033055
1101	60-61	0.9014213518311607
1101	62-63	0.7993731126949228
1101	64-65	0.8187276336751097
1101	66-67	0.8439470917110228
1101	68-69	0.820005629504017
1101	70-71	0.7680287387314166
1101	72-73	0.7728329818509323
1101	74-75	0.7741199342356495
1101	76-77	0.7302642904369634
1101	78-79	0.7314686657008735
1101	80-81	0.7236173286526082
1101	82-83	0.7052095024686551
1101	84-85	0.690592052457184
1101	86-87	0.6917064552406558
1101	88-89	0.7060342738013432
1101	90-91	0.7150069821215546
1101	92-93	0.79635771592541
1101	94-95	0.7388440535261864
1101	96-97	0.7522051722649614
1101	98-99	0.7686394305934705
1101	100	0.82822547729889
1102	1	0.8109530264423199
1102	2	0.9347891261049988
1102	3	0.8997975166788699
1102	4	0.9794865281600309
1102	5	0.8415656082857979
1102	6	0.6667912644192455
1102	7	0.7335590242643093
1102	8	0.6988259127732235
1102	9	0.9218925552432395
1102	10-11	0.9213630536319499
1102	12-13	0.8542689929246521
1102	14-15	1.0816206162256066
1102	16-17	1.199786775323254
1102	18-19	1.235765239401438
1102	20-21	0.985772086463264
1102	22-23	1.2318306622775665
1102	24-25	1.1140198478150438
1102	26-27	1.0199082971601214
1102	28-29	1.1538252541829124
1102	30-31	1.2090492670618715
1102	32-33	1.1050886752550184
1102	34-35	0.9689529764380609
1102	36-37	0.8999336626439529
1102	38-39	0.9810778028416891
1102	40-41	0.9033639919964074
1102	42-43	0.9743003068370584
1102	44-45	1.0300998828612435
1102	46-47	1.0010788894295075
1102	48-49	0.931639593882629
1102	50-51	1.0788535961733885
1102	52-53	0.9898110223740844
1102	54-55	1.0024960151315199
1102	56-57	0.8200015044136251
1102	58-59	0.7595793171766374
1102	60-61	0.8396480958513592
1102	62-63	0.8913024729916899
1102	64-65	0.7652863963117014
1102	66-67	0.804039207600745
1102	68-69	0.8713957848274916
1102	70-71	0.8073665751414225
1102	72-73	0.8030236973274967
1102	74-75	0.8057954209160911
1102	76-77	0.818692855551852
1102	78-79	0.8401876903294934
1102	80-81	0.8358523146168366
1102	82-83	0.8396992263393059
1102	84-85	0.8186095655518031
1102	86-87	0.8149669211798951
1102	88-89	0.7394797504612924
1102	90-91	0.7906841684761794
1102	92-93	0.8706692638285105
1102	94-95	0.7855567958870324
1102	96-97	0.8179441825181577
1102	98-99	0.8289384698713498
1102	100	0.8845513171006445
1103	1	0.5638015834735342
1103	2	0.7236391543900673
1103	3	0.6956478310410787
1103	4	0.7581793584400813
1103	5	0.7287269978893676
1103	6	0.5003237148158988
1103	7	0.5275181106242641
1103	8	0.5497522219448712
1103	9	0.6558859094039704
1103	10-11	0.7595847031877554
1103	12-13	0.652668881443681
1103	14-15	0.8156118962502603
1103	16-17	0.9880497078689672
1103	18-19	1.108579176796944
1103	20-21	0.8170445735552221
1103	22-23	1.068040361429503
1103	24-25	0.983333834221618
1103	26-27	0.8158545979307448
1103	28-29	0.9808491039822727
1103	30-31	1.0687257801902348
1103	32-33	0.893156178090436
1103	34-35	0.8610286089650643
1103	36-37	0.8265804575506124
1103	38-39	0.9268843145227663
1103	40-41	0.8369264559102803
1103	42-43	0.847047948697984
1103	44-45	0.9187279171302691
1103	46-47	0.8698313611083393
1103	48-49	0.7897996446697704
1103	50-51	0.8753915250547948
1103	52-53	0.8388911199783209
1103	54-55	0.8551505021638661
1103	56-57	0.7722271599712158
1103	58-59	0.7324037558462848
1103	60-61	0.6962788169716276
1103	62-63	0.656848314932688
1103	64-65	0.6753348404484569
1103	66-67	0.7263939235869898
1103	68-69	0.6934167800010513
1103	70-71	0.6504567982170713
1103	72-73	0.5607879952693153
1103	74-75	0.5790166418171125
1103	76-77	0.5719071519111694
1103	78-79	0.6222524129007283
1103	80-81	0.6691668270857747
1103	82-83	0.5791224382880529
1103	84-85	0.5949509784901679
1103	86-87	0.6712400842865733
1103	88-89	0.6423430415406273
1103	90-91	0.5832744018456744
1103	92-93	0.6859488174252633
1103	94-95	0.6542584896419186
1103	96-97	0.672620222436219
1103	98-99	0.6883179974415405
1103	100	0.716499742093891
1104	1	0.5575190772825387
1104	2	0.7021109040665259
1104	3	0.6628015586626823
1104	4	0.7269667271317246
1104	5	0.6736853851904883
1104	6	0.4608232528736025
1104	7	0.48191067753276684
1104	8	0.5091083666110592
1104	9	0.6510138789017574
1104	10-11	0.6765475722120584
1104	12-13	0.5289520208112677
1104	14-15	0.7153388251103934
1104	16-17	0.8939662126038499
1104	18-19	0.828615155701975
1104	20-21	0.713662893343276
1104	22-23	0.905952137460659
1104	24-25	0.7806623632944252
1104	26-27	0.7276512423752237
1104	28-29	0.8835737128621659
1104	30-31	0.9017215330190069
1104	32-33	0.6893604019049917
1104	34-35	0.6282817710777451
1104	36-37	0.5843561957242542
1104	38-39	0.808062532247753
1104	40-41	0.6421391323859922
1104	42-43	0.5463971655559234
1104	44-45	0.6481584500042601
1104	46-47	0.568348534289747
1104	48-49	0.5915927318061591
1104	50-51	0.6618944206535602
1104	52-53	0.6909729977822892
1104	54-55	0.6392573018135295
1104	56-57	0.6175924968003201
1104	58-59	0.6025844521677399
1104	60-61	0.6026495434938255
1104	62-63	0.5487748284047314
1104	64-65	0.519751549156382
1104	66-67	0.6032170345097541
1104	68-69	0.6077335885242832
1104	70-71	0.5150811141987859
1104	72-73	0.540444497538175
1104	74-75	0.5027784576084215
1104	76-77	0.5492444696720682
1104	78-79	0.528984743503635
1104	80-81	0.5442806359358627
1104	82-83	0.49764257690332414
1104	84-85	0.48073100885081743
1104	86-87	0.4998593402362559
1104	88-89	0.4219048193034567
1104	90-91	0.46015868952504846
1104	92-93	0.48574189448235927
1104	94-95	0.475304928957641
1104	96-97	0.48629274062459515
1104	98-99	0.5511513539269295
1104	100	0.6618988331842459
1105	1	0.43759328970534384
1105	2	0.5700646365134787
1105	3	0.5718888053320192
1105	4	0.6527414222950867
1105	5	0.52755152747838
1105	6	0.26288663339226304
1105	7	0.35971368184209496
1105	8	0.3796023943174873
1105	9	0.3971159882828701
1105	10-11	0.5313271920952332
1105	12-13	0.4188754212047954
1105	14-15	0.680479791521762
1105	16-17	0.7292441638353964
1105	18-19	0.7554716990134196
1105	20-21	0.5667039705195904
1105	22-23	0.7911535468777586
1105	24-25	0.6469293887563836
1105	26-27	0.5373535826634566
1105	28-29	0.7529501359326289
1105	30-31	0.711217201553012
1105	32-33	0.5806585348589124
1105	34-35	0.509659883539733
1105	36-37	0.4475772363480459
1105	38-39	0.539364960454435
1105	40-41	0.451682740340523
1105	42-43	0.43043501635238357
1105	44-45	0.458583414371283
1105	46-47	0.42418278413941835
1105	48-49	0.5138369118924615
1105	50-51	0.5799257761851848
1105	52-53	0.5102924630792458
1105	54-55	0.4799620178202346
1105	56-57	0.4701641162718744
1105	58-59	0.4976442776376686
1105	60-61	0.5261882370751749
1105	62-63	0.40964398510622857
1105	64-65	0.40380546054423405
1105	66-67	0.48804715810173604
1105	68-69	0.4364835385388375
1105	70-71	0.4333099506877076
1105	72-73	0.3720593475916303
1105	74-75	0.3699216009150419
1105	76-77	0.4415212360738039
1105	78-79	0.457407171173422
1105	80-81	0.5020942534717534
1105	82-83	0.43113508999252304
1105	84-85	0.4431222118599649
1105	86-87	0.4213816046621943
1105	88-89	0.3968776640405949
1105	90-91	0.37994921578951235
1105	92-93	0.4984987952002129
1105	94-95	0.4573901921486012
1105	96-97	0.4557751841051605
1105	98-99	0.4988051886191691
1105	100	0.6129106551031391
1106	1	0.42978026509948464
1106	2	0.43896166084059374
1106	3	0.430436738757507
1106	4	0.5957740874073636
1106	5	0.4778752269316229
1106	6	0.2545093212083316
1106	7	0.27771889428161245
1106	8	0.2875770983972217
1106	9	0.4111110628008632
1106	10-11	0.41130575797376423
1106	12-13	0.303100864951098
1106	14-15	0.5332736204799673
1106	16-17	0.6469852746128382
1106	18-19	0.6518762128289382
1106	20-21	0.5084011357506739
1106	22-23	0.7818564500462983
1106	24-25	0.5743737028136593
1106	26-27	0.46528333628580043
1106	28-29	0.6451346858081521
1106	30-31	0.6195116636182263
1106	32-33	0.5487450534812552
1106	34-35	0.48642974016452456
1106	36-37	0.36588520948486547
1106	38-39	0.5640756217375724
1106	40-41	0.4960350181870439
1106	42-43	0.45521325631583665
1106	44-45	0.4922325595578769
1106	46-47	0.5529012884303057
1106	48-49	0.421764831449579
1106	50-51	0.5281860869482244
1106	52-53	0.45626417574820266
1106	54-55	0.5477208488769847
1106	56-57	0.4991166178071822
1106	58-59	0.4782129255519081
1106	60-61	0.4392875675667369
1106	62-63	0.4535684667705624
1106	64-65	0.5144434960159501
1106	66-67	0.47521083778886464
1106	68-69	0.4166656427452722
1106	70-71	0.4011200441475964
1106	72-73	0.321402148657036
1106	74-75	0.33576944048228796
1106	76-77	0.3859140200812732
1106	78-79	0.46796652655266513
1106	80-81	0.4660792014495101
1106	82-83	0.4382929820190711
1106	84-85	0.36023003838350576
1106	86-87	0.3365784956697482
1106	88-89	0.3778277900602873
1106	90-91	0.38517615244252923
1106	92-93	0.4401049101938739
1106	94-95	0.41268718430960405
1106	96-97	0.3730434400275975
1106	98-99	0.4051812800209227
1106	100	0.3436232155673551
1107	1	0.4297968039068891
1107	2	0.6373177707372264
1107	3	0.5969603403746788
1107	4	0.7079206721296032
1107	5	0.5999670231125194
1107	6	0.41598069134469284
1107	7	0.39518003018897474
1107	8	0.4653255923414221
1107	9	0.5676992190877286
1107	10-11	0.6557607252240274
1107	12-13	0.5308724973229673
1107	14-15	0.7112409168592322
1107	16-17	0.8511670765177755
1107	18-19	0.944891747240554
1107	20-21	0.6954675170792655
1107	22-23	0.9670726271327723
1107	24-25	0.7220187047963478
1107	26-27	0.6235649242620127
1107	28-29	0.7786143395670919
1107	30-31	0.7333554592595561
1107	32-33	0.6547619333547843
1107	34-35	0.4846632776666908
1107	36-37	0.5225064059684215
1107	38-39	0.5874344421090072
1107	40-41	0.44300800951005925
1107	42-43	0.5854283291001607
1107	44-45	0.5086791780780331
1107	46-47	0.5253489075108888
1107	48-49	0.5512453585473622
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2213	68-69	-0.6773619512435403
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2213	100	-0.5293053872524283
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2214	3	-1.1079090192015961
2214	4	-0.7931405794547786
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2214	94-95	-1.029843381164369
2214	96-97	-0.9960051150646301
2214	98-99	-1.2009629350229751
2214	100	-1.0524616586313904
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2215	2	-2.206348745809482
2215	3	-3.707202875656435
2215	4	-2.0861442901123652
2215	5	-1.6181274357622755
2215	6	-1.4263616674663808
2215	7	-1.4048009324584783
2215	8	-1.2907948426408424
2215	9	-1.9057704113494367
2215	10-11	-1.455935918348544
2215	12-13	-1.4782252798860966
2215	14-15	-3.7435565883058715
2215	16-17	-3.4921197454796555
2215	18-19	-2.3613956107940695
2215	20-21	-1.8863729351344887
2215	22-23	-3.321088143032462
2215	24-25	-5.627874290879717
2215	26-27	-3.695239243791825
2215	28-29	-1.805997404614466
2215	30-31	-4.008830953905651
2215	32-33	-4.091999390130823
2215	34-35	-3.085534928686023
2215	36-37	-2.0484263356743995
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2215	44-45	-3.30630591425313
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2215	64-65	-1.8239953812509668
2215	66-67	-1.6907921721523422
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2215	80-81	-1.6083286107864225
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2215	84-85	-1.490650000722333
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2215	90-91	-1.6839121205755596
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2215	94-95	-2.14249164274176
2215	96-97	-1.6841220028581176
2215	98-99	-3.261972999615253
2215	100	-3.2207770078296427
2216	1	-0.5243899782538364
2216	2	-0.5271542502068058
2216	3	-0.789244333155743
2216	4	-0.6921936928157635
2216	5	-0.8772154884488401
2216	6	-0.7220709650665711
2216	7	-0.9259890378288347
2216	8	-0.7095904024142854
2216	9	-0.9410335654257054
2216	10-11	-0.9960376872059555
2216	12-13	-1.127444852216179
2216	14-15	-1.5512734115082907
2216	16-17	-1.1723201128250835
2216	18-19	-3.0688162081970134
2216	20-21	-1.4683079020130805
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2216	24-25	-1.2323277505030745
2216	26-27	-1.1029769973952952
2216	28-29	-1.0876261376623049
2216	30-31	-1.2961107213138376
2216	32-33	-1.1885576858197524
2216	34-35	-1.5286000388305183
2216	36-37	-1.4837793980474814
2216	38-39	-1.5388022471830283
2216	40-41	-1.5506232693532027
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2216	54-55	-1.3049798787052893
2216	56-57	-1.4160167917448874
2216	58-59	-1.3999027820172856
2216	60-61	-1.41184900103314
2216	62-63	-1.4636493272608533
2216	64-65	-1.519337343355808
2216	66-67	-1.4162555256140479
2216	68-69	-1.6102532533159781
2216	70-71	-1.6335256950776937
2216	72-73	-1.5951408308187425
2216	74-75	-1.5778246406717145
2216	76-77	-1.3726332603303035
2216	78-79	-1.5385493913623733
2216	80-81	-1.5480451549168208
2216	82-83	-1.6541752077702405
2216	84-85	-1.4650894441326798
2216	86-87	-1.5142926315500027
2216	88-89	-1.4253865395717575
2216	90-91	-1.4089405917846811
2216	92-93	-1.3442996685893789
2216	94-95	-1.3417591979665637
2216	96-97	-1.3048850252994662
2216	98-99	-1.3147245174917153
2216	100	-1.226817157824886
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	19480.0
1	3134.0
2	520.0
3	510.0
4	487.0
5	243.0
6	237.0
7	329.0
8	630.0
9	1040.0
10	1716.0
11	2976.0
12	4792.0
13	6960.0
14	8918.0
15	11396.0
16	14320.0
17	17118.0
18	19963.0
19	23190.0
20	26182.0
21	30463.0
22	35456.0
23	41599.0
24	46605.0
25	52736.0
26	59378.0
27	68616.0
28	81948.0
29	97636.0
30	115856.0
31	139923.0
32	174169.0
33	223481.0
34	315775.0
35	429363.0
36	632850.0
37	980759.0
38	757044.0
39	286164.0
40	221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60203079735423	22.227250286215607	12.832849657757391	23.337869258672768
2	15.130455409479406	19.939263459648902	41.60472109588018	23.325560034991515
3	18.170985835018225	27.818786376589255	29.068627721492067	24.941600066900453
4	11.895235865943912	17.36838854984787	41.255780008560855	29.48059557564736
5	12.820357792622161	39.02734783376102	35.20530816803117	12.94698620558565
6	28.48419035506166	39.491785386346436	18.744117788148255	13.27990647044365
7	26.07776226602876	32.05862422108658	24.669455384756482	17.19415812812818
8	25.80396083199424	35.13019309071895	20.278867063543878	18.78697901374294
9	25.93268671357774	15.123598082223195	22.444125003715797	36.49959020048327
10-11	25.581652650699212	26.126346617506634	27.54475523280069	20.747245498993465
12-13	25.856103021911075	24.025247945103228	27.527361372708096	22.591287660277594
14-15	23.296209897244744	25.048227411049375	25.18419531812148	26.47136737358441
16-17	21.85179742417231	27.55075300498011	26.675947608513432	23.921501962334144
18-19	21.140749507246813	28.449105070173598	28.437542106865354	21.972603315714238
20-21	23.21715706677313	27.482873512448293	27.03343328208482	22.266536138693755
22-23	23.89378342472312	27.040105475310217	26.764582856377455	22.301528243589207
24-25	23.149103230785713	27.228375117230375	27.56347375000544	22.059047901978474
26-27	22.98509367130962	27.47202376333394	26.377368613304114	23.165513952052322
28-29	21.84779966219695	28.145851933556198	27.153133489530973	22.853214914715878
30-31	22.853953719066876	27.506252207527957	27.032786229123435	22.607007844281735
32-33	23.480604738742368	27.881760334839196	25.80645846232507	22.83117646409337
34-35	23.402268412535943	27.590602715639346	26.976271071263234	22.030857800561478
36-37	22.98328113666606	27.15645512945092	26.653540505781987	23.20672322810103
38-39	23.220531539751864	27.087795580812735	27.3928999547966	22.298772924638804
40-41	22.6990684463561	26.10392287271464	28.204965376422624	22.992043304506634
42-43	22.618698710433762	26.487110709006572	27.3152088961381	23.578981684421567
44-45	22.86520376441882	27.647180891419946	27.04730841675722	22.440306927404013
46-47	22.841427256535816	27.297273675241758	26.43336774528177	23.427931322940648
48-49	23.64970344946495	26.67428108896856	26.901694538085273	22.774320923481213
50-51	23.296869949553408	26.587610873562404	26.666538536195016	23.448980640689168
52-53	22.487797626947682	27.341608048204286	26.597159769596264	23.573434555251772
54-55	22.530118754781757	27.2740980567158	26.49126262768382	23.704520560818622
56-57	23.868369208782962	26.527199495883885	26.26895146926808	23.335479826065075
58-59	22.54195272769977	26.960736948577075	27.391227435696123	23.10608288802703
60-61	22.555435580060283	27.78692989077983	26.470384632944	23.18724989621589
62-63	23.1750480673782	26.877186388889772	25.92703950602342	24.020726037708606
64-65	22.071889988871344	27.13613432279727	26.555195359878375	24.236780328453012
66-67	23.12920467939454	26.5887199363532	26.477892282698217	23.80418310155404
68-69	22.242835543397838	26.839404386527487	26.851489888587178	24.066270181487496
70-71	22.32878930499567	26.39628662789988	27.016130530784345	24.258793536320105
72-73	23.064901190228042	26.64788165869723	27.388443928333384	22.89877322274134
74-75	23.284092303941616	27.048269248003454	26.83689193051933	22.830746517535598
76-77	22.855928580126896	27.026674371749287	26.409988182260523	23.707408865863293
78-79	22.697959210982848	26.969832040787956	26.559781697311053	23.772427050918143
80-81	22.211741572228448	27.342488093803468	27.400268516517905	23.045501817450177
82-83	23.337364762127123	26.170927272153932	28.47386371732135	22.017844248397594
84-85	23.651311510659593	26.88595524856452	26.717875886308313	22.74485735446758
86-87	22.043247193908552	27.707268608425963	27.258775403085238	22.99070879458025
88-89	23.277957607468753	26.14461340211966	27.07009107395758	23.507337916454006
90-91	22.89748294148157	26.237030245345583	27.33480678865869	23.53068002451416
92-93	23.107166098664326	26.606558157822853	27.25356184134848	23.032713902164346
94-95	21.760418744663067	26.575770921534712	27.9892392002211	23.674571133581118
96-97	22.260101397063465	26.441136491997337	27.42948929534519	23.86927281559401
98-99	21.54542185106104	27.45884554388036	27.235545166003845	23.760187439054757
100	22.504277417541726	26.5652828306211	26.290592756536142	24.639846995301035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16152.0
1	9517.5
2	2591.5
3	1771.0
4	914.5
5	499.5
6	391.5
7	371.0
8	384.5
9	366.0
10	356.5
11	354.0
12	329.5
13	323.5
14	332.5
15	425.0
16	523.5
17	618.5
18	792.0
19	1034.5
20	1288.5
21	1568.5
22	2057.5
23	2813.0
24	3983.0
25	5620.5
26	7699.5
27	10081.5
28	14563.5
29	24822.0
30	39180.0
31	52780.0
32	71586.5
33	89346.5
34	102610.0
35	117088.0
36	130724.5
37	157683.0
38	167162.5
39	149019.5
40	133628.5
41	119995.0
42	115289.0
43	122463.0
44	134955.5
45	165848.5
46	196978.0
47	218015.5
48	230903.0
49	224195.0
50	212684.5
51	218909.5
52	222232.5
53	211984.5
54	199996.0
55	170226.5
56	146124.5
57	117458.0
58	87912.0
59	83501.5
60	68664.0
61	45483.0
62	33628.0
63	28618.5
64	20376.0
65	8502.5
66	3918.0
67	2584.0
68	1829.5
69	1344.5
70	996.0
71	766.0
72	589.0
73	451.5
74	354.0
75	297.0
76	231.0
77	148.5
78	89.0
79	70.0
80	61.0
81	34.0
82	26.5
83	23.5
84	19.0
85	20.0
86	17.0
87	12.0
88	12.5
89	13.5
90	11.0
91	7.5
92	6.0
93	4.5
94	3.5
95	2.5
96	2.0
97	2.5
98	2.0
99	1.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.996095841668985
2	0.44442726442978653
3	0.4795970075512502
4	0.4654657413961395
5	0.43071845765151034
6	0.48466652007326294
7	0.5021985842118903
8	0.5050501850055226
9	0.5188434836591657
10-11	0.5324783600464958
12-13	0.5307462766014749
14-15	0.527725692057109
16-17	0.5333549632534272
18-19	0.5319291628566112
20-21	0.5302498868336945
22-23	0.4925348259668036
24-25	0.52749333939985
26-27	0.5084192985357769
28-29	0.5140908156697788
30-31	0.5163826577891053
32-33	0.5191814511606332
34-35	0.5315172649641976
36-37	0.5497886330122853
38-39	0.5379280860076596
40-41	0.5440959929094418
42-43	0.5412338306313887
44-45	0.5512883638000474
46-47	0.5498625634032314
48-49	0.5217162074216396
50-51	0.5373894503021958
52-53	0.5292993532358171
54-55	0.5392799560135297
56-57	0.5270286340853321
58-59	0.5333972091911107
60-61	0.5257084485327247
62-63	0.5206389360107119
64-65	0.5316334412928271
66-67	0.5295422673774969
68-69	0.5505173754373247
70-71	0.5334711395820567
72-73	0.5378647171011344
74-75	0.5345167265397218
76-77	0.5225294417220459
78-79	0.5539392963896833
80-81	0.5275567083063751
82-83	0.5266906665838647
84-85	0.5364283552198975
86-87	0.8406413524783474
88-89	1.1076568016065285
90-91	1.0982148345342797
92-93	1.0980564122679668
94-95	1.1023866208805193
96-97	1.0962292754631582
98-99	1.0920786120857602
100	1.0867978698753302
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4734183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.641486285791668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.35166021013984	15.874389484777915
2	12.361395008486951	5.3503792109884785
3	5.16010795709304	3.3501721675990046
4	2.585550613624383	2.2382063258388922
5	1.514510110111995	1.6388124888840787
6	0.9610261480483427	1.247882052196524
7	0.6449235172775493	0.9769972418182625
8	0.4439158081559148	0.7685598299401883
9	0.3589586399307971	0.6991558634905897
>10	1.9027671059579427	7.794989694474855
>50	0.2521782170488674	3.8734008097765784
>100	0.33473901667588046	16.328455980976774
>500	0.06760183704066218	10.25451799606162
>1k	0.05646511822182313	22.087727492174132
>5k	0.003419168058414895	4.874214234744404
>10k+	7.815241276376905E-4	2.6421391262576988
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	20058	0.42368450902721755	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	18222	0.3849027382338199	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	17691	0.3736864417788666	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	15676	0.331123659562801	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	14354	0.3031990947540473	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	13899	0.29358814393106475	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	13701	0.28940579610040423	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	11372	0.24021040166803861	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	9265	0.1957043063185348	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	8815	0.18619897033976085	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	8812	0.18613560143323568	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	8633	0.1823545900105678	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	8620	0.18207999141562548	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	8093	0.17094818683603907	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	7979	0.16854016838808303	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7655	0.16169632648336577	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7383	0.15595087895841794	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7266	0.15347949160393673	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	7148	0.15098698128061377	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7110	0.15018430846462844	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	7000	0.14786078189203924	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	6721	0.14196747358519937	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	6548	0.13831319997558184	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6390	0.13497577089859011	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6347	0.13406748323839615	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6269	0.132419891668742	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6062	0.128047437118506	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6009	0.12692791976989481	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	5943	0.12553380382634133	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5940	0.12547043491981616	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5886	0.12432979460236329	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5885	0.12430867163352155	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5811	0.12274557193923429	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5744	0.12133033302683906	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5576	0.11778167426143012	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	5574	0.11773942832374668	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	5439	0.11488782753011448	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	5219	0.11024077438493611	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	5141	0.10859318281528196	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	5116	0.10806510859423897	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	5070	0.10709345202751985	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	5066	0.10700896015215296	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5015	0.10593168874122524	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	4937	0.10428409717157111	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	4910	0.10371377701284468	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4873	0.10293222716570102	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA	4871	0.10288998122801758	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	4797	0.10132688153373032	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1122968841719892E-5
2	0.0	0.0	0.0	0.0	2.1122968841719892E-5
3	0.0	0.0	0.0	0.0	2.1122968841719892E-5
4	0.0	0.0	0.0	0.0	2.1122968841719892E-5
5	0.0	0.0	0.0	0.0	2.1122968841719892E-5
6	0.0	0.0	0.0	0.0	2.1122968841719892E-5
7	0.0	0.0	0.0	0.0	2.1122968841719892E-5
8	0.0	0.0	0.0	0.0	2.1122968841719892E-5
9	0.0	0.0	0.0	0.0	2.1122968841719892E-5
10-11	0.0	0.0	0.0	0.0	2.1122968841719892E-5
12-13	0.0	0.0	0.0	0.0	5.2807422104299726E-5
14-15	0.0	0.0	0.0	1.0561484420859946E-5	1.161763286294594E-4
16-17	0.0	0.0	0.0	3.168445326257984E-5	1.6898375073375914E-4
18-19	0.0	0.0	0.0	4.2245937683439785E-5	2.0066820399633896E-4
20-21	0.0	0.0	0.0	5.2807422104299726E-5	2.534756261006387E-4
22-23	0.0	0.0	0.0	1.0561484420859945E-4	2.745985949423586E-4
24-25	0.0	0.0	0.0	2.323526572589188E-4	2.745985949423586E-4
26-27	0.0	0.0	0.0	4.963897677804174E-4	2.8516007936321854E-4
28-29	0.0	0.0	0.0	0.0012568166460823335	2.957215637840785E-4
30-31	0.0	0.0	0.0	0.004826598380332996	2.957215637840785E-4
32-33	0.0	0.0	0.0	0.014881131548991663	2.957215637840785E-4
34-35	0.0	0.0	0.0	0.031283116854587165	3.168445326257984E-4
36-37	0.0	0.0	0.0	0.06033776049637288	3.168445326257984E-4
38-39	0.0	0.0	0.0	0.10505508553429388	3.2740601704665833E-4
40-41	0.0	0.0	0.0	0.15606705528704742	3.379675014675183E-4
42-43	0.0	0.0	0.0	0.21197955381107997	3.485289858883782E-4
44-45	0.0	0.0	0.0	0.2803123580140438	3.80213439150958E-4
46-47	0.0	0.0	0.0	0.3648464793186068	4.013364079926779E-4
48-49	0.0	0.0	0.0	0.47041907758952284	4.3302086125525776E-4
50-51	0.0	0.0	0.0	0.5842085107398679	4.858282833595575E-4
52-53	0.0	0.0	0.0	0.6951780275498434	5.069512522012774E-4
54-55	0.0	0.0	0.0	0.8195395066054691	5.280742210429973E-4
56-57	0.0	0.0	0.0	0.9766521488501818	5.703201587264371E-4
58-59	0.0	0.0	0.0	1.1672024507713368	5.91443127568157E-4
60-61	0.0	0.0	0.0	1.369201401804704	5.91443127568157E-4
62-63	0.0	0.0	0.0	1.5800931227204358	6.125660964098769E-4
64-65	0.0	0.0	0.0	1.8036269404879364	6.125660964098769E-4
66-67	0.0	0.0	0.0	2.057260988854888	6.231275808307368E-4
68-69	0.0	0.0	0.0	2.3483143765249466	6.336890652515968E-4
70-71	0.0	0.0	0.0	2.6761745374017014	6.442505496724567E-4
72-73	0.0	0.0	0.0	3.0251787900890186	6.970579717767565E-4
74-75	0.0	0.0	0.0	3.369176899160848	7.393039094601962E-4
76-77	0.0	0.0	0.0	3.768284833940724	7.393039094601962E-4
78-79	0.0	0.0	0.0	4.1955285632177715	7.393039094601962E-4
80-81	0.0	0.0	0.0	4.661849784852	7.393039094601962E-4
82-83	0.0	0.0	0.0	5.148713093684803	7.70988362722776E-4
84-85	0.0	0.0	0.0	5.626947669745761	7.815498471436359E-4
86-87	0.0	0.0	0.0	6.138366429857063	8.026728159853558E-4
88	0.0	0.0	0.0	6.548627292185367	8.237957848270757E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4895	0.0	43.695427	94
GTACTAG	5635	0.0	36.587677	1
ACGTTAA	990	0.0	34.61886	8
TATTCTC	2780	0.0	34.31086	5
TACCCCG	290	0.0	34.025272	5
TAGGCAT	6980	0.0	33.92778	5
GTATCAA	11775	0.0	32.414173	1
CAACGCA	10585	0.0	32.094288	5
GCATCGT	6300	0.0	31.299236	8
CTAGGCA	7890	0.0	30.671402	4
GGTATCA	7685	0.0	29.897331	1
ATCAACG	11480	0.0	29.431574	3
TCAAGAC	1345	0.0	29.348337	3
AGGGCTA	6440	0.0	27.579493	5
CTGTGCG	8390	0.0	27.197952	9
TATCAAC	14075	0.0	27.044376	2
CGTTAAC	1255	0.0	26.93709	9
AACGCAG	13590	0.0	26.875273	6
TCTCTGC	3515	0.0	26.846998	8
TAGTACT	8430	0.0	26.532782	4
>>END_MODULE