##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146430_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5049260
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.670377441446867	31.0	31.0	34.0	27.0	34.0
2	30.65937662152474	31.0	31.0	34.0	27.0	34.0
3	30.906563337994083	31.0	31.0	34.0	27.0	34.0
4	34.3769576135909	37.0	35.0	37.0	30.0	37.0
5	34.58613143312089	37.0	35.0	37.0	31.0	37.0
6	34.73911404839521	37.0	35.0	37.0	31.0	37.0
7	34.606369250147544	37.0	35.0	37.0	30.0	37.0
8	34.550039213666956	37.0	35.0	37.0	30.0	37.0
9	35.95631795550239	39.0	35.0	39.0	30.0	39.0
10-11	35.89455029053762	39.0	35.0	39.0	30.0	39.0
12-13	35.88883895858007	39.0	35.0	39.0	30.0	39.0
14-15	36.92676194135379	40.0	36.0	41.0	30.0	41.0
16-17	36.76231725044858	40.0	36.0	41.0	29.5	41.0
18-19	36.7116043737102	40.0	36.0	41.0	29.5	41.0
20-21	37.047010056919234	40.0	36.0	41.0	30.0	41.0
22-23	36.75785422022237	40.0	36.0	41.0	29.5	41.0
24-25	36.86745681545415	40.0	36.0	41.0	30.0	41.0
26-27	36.94203487243675	40.0	36.0	41.0	30.0	41.0
28-29	36.77774059961261	40.0	36.0	41.0	29.5	41.0
30-31	36.72413244713087	40.0	36.0	41.0	30.0	41.0
32-33	36.831201106696824	40.0	36.0	41.0	30.0	41.0
34-35	36.877921319163605	40.0	36.0	41.0	30.0	41.0
36-37	36.812056024051046	40.0	35.5	41.0	30.0	41.0
38-39	36.64107542491375	39.5	35.0	41.0	30.0	41.0
40-41	36.603268795823546	39.0	35.0	41.0	30.0	41.0
42-43	36.442341650063575	39.0	35.0	41.0	29.0	41.0
44-45	36.24929544131219	39.0	35.0	41.0	28.5	41.0
46-47	36.118807409402564	39.0	35.0	41.0	28.0	41.0
48-49	35.98380376530422	39.0	35.0	40.5	28.0	41.0
50-51	35.75980222844536	38.0	34.0	40.0	28.0	41.0
52-53	35.56820246927273	38.0	34.0	40.0	27.5	41.0
54-55	35.36096952820809	38.0	34.0	40.0	27.0	41.0
56-57	35.20823615737751	37.0	34.0	40.0	27.0	41.0
58-59	34.937805737870505	37.0	33.0	40.0	27.0	41.0
60-61	34.68203598151016	36.0	33.0	40.0	26.5	41.0
62-63	34.408507484265016	36.0	33.0	39.0	26.0	41.0
64-65	34.115994521969554	35.0	33.0	39.0	26.0	41.0
66-67	33.82341828307514	35.0	32.5	39.0	26.0	40.5
68-69	33.53161591995659	35.0	32.0	38.0	26.0	40.0
70-71	33.47023415312343	35.0	32.0	37.5	26.0	40.0
72-73	33.26646439280211	35.0	32.0	37.0	26.0	40.0
74-75	33.00354834173721	35.0	32.0	36.5	25.0	39.0
76-77	32.77776585083755	35.0	32.0	36.0	25.0	39.0
78-79	32.56427714160095	35.0	32.0	36.0	25.0	39.0
80-81	32.35491943373881	35.0	32.0	35.5	25.0	37.5
82-83	32.12170892368387	35.0	31.0	35.0	24.0	37.0
84-85	31.953906513033594	35.0	31.0	35.0	24.0	37.0
86-87	31.79395911480098	35.0	31.5	35.0	24.0	36.0
88-89	31.798805072426454	35.0	32.0	35.0	24.0	36.0
90-91	31.723015055671524	35.0	32.0	35.0	24.0	36.0
92-93	31.584507432772327	35.0	32.0	35.0	24.0	36.0
94-95	31.568115822912667	35.0	32.0	35.0	24.0	35.0
96-97	31.481312212086525	35.0	31.5	35.0	24.0	35.0
98-99	31.403242257281264	35.0	31.0	35.0	23.5	35.0
100	30.913215203812044	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7651644594701743
1101	2	0.9035559740871832
1101	3	0.9085407963081629
1101	4	0.9406601815410411
1101	5	0.858024981342119
1101	6	0.5759112219365505
1101	7	0.6322941852568462
1101	8	0.6825533174186589
1101	9	0.8914169815637933
1101	10-11	0.9406600173771054
1101	12-13	0.7810391568569202
1101	14-15	1.014224611779028
1101	16-17	1.1684991818238828
1101	18-19	1.2637270314015865
1101	20-21	0.9665098494201558
1101	22-23	1.265833689615235
1101	24-25	1.1132081190735832
1101	26-27	1.0225512334898
1101	28-29	1.1828549743182961
1101	30-31	1.1899425748820178
1101	32-33	1.0822108815445262
1101	34-35	0.929330914130226
1101	36-37	0.9220999905227885
1101	38-39	0.9813465200120675
1101	40-41	0.9371787012925594
1101	42-43	0.9398688719886579
1101	44-45	0.9448494097660287
1101	46-47	0.9845271929768558
1101	48-49	0.9550317352839031
1101	50-51	0.9689839901403019
1101	52-53	0.9126922716145316
1101	54-55	0.917383357509955
1101	56-57	0.7754967428888406
1101	58-59	0.7901659449464375
1101	60-61	0.8197773331541711
1101	62-63	0.7661452628601921
1101	64-65	0.7342045329843856
1101	66-67	0.7183817661825103
1101	68-69	0.7561331650166636
1101	70-71	0.6868499512799389
1101	72-73	0.687706282765383
1101	74-75	0.6989658269342911
1101	76-77	0.7429814267813768
1101	78-79	0.7399814665639539
1101	80-81	0.747438974879735
1101	82-83	0.7792001399627537
1101	84-85	0.7622062328498274
1101	86-87	0.7366748626709203
1101	88-89	0.8040239191424945
1101	90-91	0.7538549319194701
1101	92-93	0.8320245348939928
1101	94-95	0.7916376979122646
1101	96-97	0.7681184723264067
1101	98-99	0.7709066779873162
1101	100	0.7866424656867927
1102	1	0.7189543557166971
1102	2	0.8621191944247784
1102	3	0.803413578695853
1102	4	0.9289307839326213
1102	5	0.7466982694397828
1102	6	0.5686720402769367
1102	7	0.5960353510596619
1102	8	0.613748410566842
1102	9	0.8068852221733422
1102	10-11	0.8452958923483038
1102	12-13	0.7907223864456796
1102	14-15	1.00278680754748
1102	16-17	1.1162684745524558
1102	18-19	1.1210584409700957
1102	20-21	0.917720985251826
1102	22-23	1.164897218408214
1102	24-25	1.083500244759179
1102	26-27	0.9893290453271746
1102	28-29	1.114544597285196
1102	30-31	1.1880282742497386
1102	32-33	1.0062702511541701
1102	34-35	0.9150273116016407
1102	36-37	0.846634249594949
1102	38-39	0.9492816027109754
1102	40-41	0.8869613391175406
1102	42-43	0.8661346278678721
1102	44-45	0.869896554129987
1102	46-47	0.8679750700245563
1102	48-49	0.845387177339596
1102	50-51	0.9275805256615612
1102	52-53	0.9525271274231102
1102	54-55	0.9754474341303734
1102	56-57	0.8655471420119198
1102	58-59	0.796726324524272
1102	60-61	0.834666158225339
1102	62-63	0.845657885001259
1102	64-65	0.7666909169778791
1102	66-67	0.838446567677245
1102	68-69	0.7699155305131811
1102	70-71	0.7615272078308806
1102	72-73	0.7085571407085354
1102	74-75	0.7054139377639714
1102	76-77	0.6956569205394274
1102	78-79	0.6249751914951744
1102	80-81	0.734107161157084
1102	82-83	0.6600074653984507
1102	84-85	0.5834900213126772
1102	86-87	0.6641224023116514
1102	88-89	0.6697759019924803
1102	90-91	0.7016856070187529
1102	92-93	0.6646587054760751
1102	94-95	0.6574014783254114
1102	96-97	0.6208064811797556
1102	98-99	0.6578633205243243
1102	100	0.7109343323933537
1103	1	0.5857833277952871
1103	2	0.794527104203123
1103	3	0.7502365527359771
1103	4	0.7903497108627491
1103	5	0.7297257484305248
1103	6	0.5425856722076645
1103	7	0.5872383486050481
1103	8	0.6279008139076865
1103	9	0.6616226790287172
1103	10-11	0.7733980579718036
1103	12-13	0.6794288353634599
1103	14-15	0.8113252219045677
1103	16-17	1.0435732484673323
1103	18-19	1.1757890450862618
1103	20-21	0.8322214833995787
1103	22-23	1.1009341849061869
1103	24-25	1.0124905030130265
1103	26-27	0.848471270557333
1103	28-29	1.0489719760805372
1103	30-31	1.101936271998433
1103	32-33	0.894954608788332
1103	34-35	0.8645014535156506
1103	36-37	0.8143507418715146
1103	38-39	0.9257686800173772
1103	40-41	0.8668203556050429
1103	42-43	0.8034258134226846
1103	44-45	0.8971859348779176
1103	46-47	0.8584159429589135
1103	48-49	0.809170779613062
1103	50-51	0.8557339141124558
1103	52-53	0.9391304664013731
1103	54-55	0.8520117207197586
1103	56-57	0.8205432659210246
1103	58-59	0.7767775499835352
1103	60-61	0.7365447694017746
1103	62-63	0.7145035955286616
1103	64-65	0.7812861987402755
1103	66-67	0.8826133107778915
1103	68-69	0.8275876803362365
1103	70-71	0.813665598027363
1103	72-73	0.7458788921778847
1103	74-75	0.7783891786823247
1103	76-77	0.7925580743656937
1103	78-79	0.7600951377891718
1103	80-81	0.7946140355434466
1103	82-83	0.7195370760088977
1103	84-85	0.6959318092176758
1103	86-87	0.7231192231407171
1103	88-89	0.7290306957187767
1103	90-91	0.6849290476346823
1103	92-93	0.801141123051405
1103	94-95	0.7487254959689622
1103	96-97	0.7412161822108345
1103	98-99	0.7977591786274125
1103	100	0.8718185548545208
1104	1	0.5799580999523677
1104	2	0.7434375675734302
1104	3	0.6785786875881854
1104	4	0.7796432478685489
1104	5	0.663242131355922
1104	6	0.37354916941084326
1104	7	0.3759035480463382
1104	8	0.4037802389921126
1104	9	0.6312687010852898
1104	10-11	0.6097541387985785
1104	12-13	0.503170824488393
1104	14-15	0.7215279907751864
1104	16-17	0.8733393868333437
1104	18-19	0.8091115287567732
1104	20-21	0.6841870664619023
1104	22-23	0.9176359083590881
1104	24-25	0.7373092016768368
1104	26-27	0.6602602640622095
1104	28-29	0.815779786580606
1104	30-31	0.9212201759179521
1104	32-33	0.7252618552630778
1104	34-35	0.6178211098569975
1104	36-37	0.6266192289243406
1104	38-39	0.7370978560375434
1104	40-41	0.6722297211754338
1104	42-43	0.6368952749939254
1104	44-45	0.6429679893129716
1104	46-47	0.6113946894057705
1104	48-49	0.6449044944459459
1104	50-51	0.6992379387677161
1104	52-53	0.6873238371198838
1104	54-55	0.6315591765032877
1104	56-57	0.6211181147132621
1104	58-59	0.61466816731037
1104	60-61	0.683649389223568
1104	62-63	0.501234451341162
1104	64-65	0.4964112442145705
1104	66-67	0.6677402564164296
1104	68-69	0.6009990526686337
1104	70-71	0.5174681331162319
1104	72-73	0.5250774170427945
1104	74-75	0.5850228471831613
1104	76-77	0.5693952424688078
1104	78-79	0.5203928886809095
1104	80-81	0.5130096290481561
1104	82-83	0.4698257346605672
1104	84-85	0.504071007576222
1104	86-87	0.4359007218006461
1104	88-89	0.3915016465159198
1104	90-91	0.4923375470654179
1104	92-93	0.46119754915544675
1104	94-95	0.4476925975912138
1104	96-97	0.3923013056403164
1104	98-99	0.5000743866724839
1104	100	0.628773208371161
1105	1	0.4873477434468647
1105	2	0.5948309166021275
1105	3	0.6506563187731054
1105	4	0.7106934089205126
1105	5	0.6268358259423152
1105	6	0.3330910011263555
1105	7	0.3762845704524054
1105	8	0.47285241900024033
1105	9	0.48092492879304416
1105	10-11	0.5546971112278172
1105	12-13	0.5200459781755455
1105	14-15	0.7206835737070065
1105	16-17	0.8534176769963082
1105	18-19	0.8503872315381926
1105	20-21	0.6619890622239879
1105	22-23	0.9085410780094136
1105	24-25	0.7371179803681471
1105	26-27	0.5695620191999211
1105	28-29	0.8253263455709003
1105	30-31	0.8260573953552068
1105	32-33	0.6825959684953062
1105	34-35	0.5876626183324163
1105	36-37	0.5424108425359648
1105	38-39	0.6402138808092772
1105	40-41	0.5094588970227463
1105	42-43	0.6080212631566653
1105	44-45	0.6045284485200355
1105	46-47	0.5616860761893605
1105	48-49	0.6310417103935393
1105	50-51	0.6641302137108838
1105	52-53	0.6250832792933423
1105	54-55	0.5641328185175212
1105	56-57	0.5574155507854854
1105	58-59	0.5600966491207089
1105	60-61	0.6208620500422057
1105	62-63	0.5970681803047881
1105	64-65	0.5471760888718578
1105	66-67	0.6230240917335905
1105	68-69	0.5882793358344287
1105	70-71	0.5690737428697616
1105	72-73	0.5448113546557494
1105	74-75	0.5412475724939938
1105	76-77	0.5257674668686931
1105	78-79	0.543945470178727
1105	80-81	0.6323516086279568
1105	82-83	0.5664204939996722
1105	84-85	0.5237420501168302
1105	86-87	0.5295042117873443
1105	88-89	0.5560764433648302
1105	90-91	0.6499809011662947
1105	92-93	0.6404954737803727
1105	94-95	0.49712792157387753
1105	96-97	0.5246556670280995
1105	98-99	0.5891052020117407
1105	100	0.7275844103487437
1106	1	0.5143250439494409
1106	2	0.6189319596795322
1106	3	0.5468441654478227
1106	4	0.7672436192532004
1106	5	0.6395623926312126
1106	6	0.2998999527685129
1106	7	0.33749928809353946
1106	8	0.3924160201063316
1106	9	0.5586358260337789
1106	10-11	0.5548900345501977
1106	12-13	0.4792375431875655
1106	14-15	0.6472511744255911
1106	16-17	0.7451163489693116
1106	18-19	0.8246585430486704
1106	20-21	0.5354149990910315
1106	22-23	0.8321583399673216
1106	24-25	0.6755270444525863
1106	26-27	0.5861319767591695
1106	28-29	0.8127586783469098
1106	30-31	0.7763160919209753
1106	32-33	0.6776287141065396
1106	34-35	0.586934194237493
1106	36-37	0.521443146743799
1106	38-39	0.6678007379973394
1106	40-41	0.5451014850690328
1106	42-43	0.6259645134300982
1106	44-45	0.6470673207983921
1106	46-47	0.599677522210257
1106	48-49	0.5650697727627332
1106	50-51	0.647211297639295
1106	52-53	0.5941970146678273
1106	54-55	0.6214619929914562
1106	56-57	0.5947369937509279
1106	58-59	0.5652367684331807
1106	60-61	0.528994711257333
1106	62-63	0.44909257694442317
1106	64-65	0.5192870845758577
1106	66-67	0.5550159258044189
1106	68-69	0.5262395985450468
1106	70-71	0.577252747714212
1106	72-73	0.47597129481850686
1106	74-75	0.5280514850460918
1106	76-77	0.5618532548517337
1106	78-79	0.5345628258272299
1106	80-81	0.554753172311905
1106	82-83	0.5377569829843551
1106	84-85	0.5165316362775698
1106	86-87	0.5104288441381648
1106	88-89	0.518179569261612
1106	90-91	0.5189779571662285
1106	92-93	0.6368461417733435
1106	94-95	0.5935803967446844
1106	96-97	0.575324419293672
1106	98-99	0.5864526715756817
1106	100	0.6118032995266596
1107	1	0.47558684760292635
1107	2	0.6393035899675326
1107	3	0.5395945649777865
1107	4	0.6839538666100324
1107	5	0.4983699898640239
1107	6	0.3740575732347935
1107	7	0.3594537257668904
1107	8	0.39295764533384414
1107	9	0.5171462712427655
1107	10-11	0.49232764833932663
1107	12-13	0.4526615522270987
1107	14-15	0.6845548844641272
1107	16-17	0.7361685074114988
1107	18-19	0.8662590722370282
1107	20-21	0.5857506265564894
1107	22-23	0.8002156245282492
1107	24-25	0.5314343555668728
1107	26-27	0.5218573075678776
1107	28-29	0.6726005228623819
1107	30-31	0.6177903047351805
1107	32-33	0.5735894706479812
1107	34-35	0.47884878720024204
1107	36-37	0.4807756779596275
1107	38-39	0.5137461501027829
1107	40-41	0.41131707007469487
1107	42-43	0.5479806862475769
1107	44-45	0.4803515034088335
1107	46-47	0.47633818504561987
1107	48-49	0.4990918539627174
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2213	28-29	-1.0156004444792188
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2214	3	-1.1520776522374838
2214	4	-0.9027159306603068
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2214	12-13	-1.4287349979789639
2214	14-15	-3.9536018617933877
2214	16-17	-3.268944912129797
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2214	20-21	-1.6119669270782566
2214	22-23	-2.873036384939354
2214	24-25	-2.2377595000123804
2214	26-27	-1.5830020055668896
2214	28-29	-1.1371694762493334
2214	30-31	-1.2101935136970852
2214	32-33	-1.3327562633146144
2214	34-35	-1.1930652645981823
2214	36-37	-1.2647000195755638
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2214	42-43	-4.049522439562033
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2214	64-65	-1.2319816586326269
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2214	78-79	-1.1722457643193351
2214	80-81	-1.1861705562544742
2214	82-83	-1.1831430735361792
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2214	86-87	-1.0673416438548138
2214	88-89	-1.1058158255208426
2214	90-91	-1.0599930607265478
2214	92-93	-1.066097673722581
2214	94-95	-1.1563608635666824
2214	96-97	-1.0777627917702475
2214	98-99	-1.2674647076213041
2214	100	-1.1672231017328656
2215	1	-4.115606661215317
2215	2	-2.3184190099305333
2215	3	-3.9211157786156896
2215	4	-2.2027069119750706
2215	5	-1.6179683439092898
2215	6	-1.526491134546859
2215	7	-1.4057478007640256
2215	8	-1.3265133604414387
2215	9	-1.9145347081276896
2215	10-11	-1.6009267628495678
2215	12-13	-1.5717313168750024
2215	14-15	-3.8656282395972212
2215	16-17	-3.6300034304760302
2215	18-19	-2.5295122871184432
2215	20-21	-1.9930088131249306
2215	22-23	-3.404983648940359
2215	24-25	-5.757367186555349
2215	26-27	-3.8264171803845883
2215	28-29	-2.000863866055738
2215	30-31	-4.223426827278914
2215	32-33	-4.195762872861636
2215	34-35	-3.1423463279953197
2215	36-37	-2.1629950919970966
2215	38-39	-2.0096089224765237
2215	40-41	-2.2119718723207384
2215	42-43	-2.0216475761895936
2215	44-45	-3.5394398624311876
2215	46-47	-2.89826140178468
2215	48-49	-2.16313800741046
2215	50-51	-5.2808407127590975
2215	52-53	-5.675342485316484
2215	54-55	-3.2780556697810255
2215	56-57	-2.0965046339696087
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2215	62-63	-2.897064607945648
2215	64-65	-2.0734260461989678
2215	66-67	-1.882657823578299
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2215	70-71	-1.6675941733522386
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2215	86-87	-1.6010561059171842
2215	88-89	-1.540305076403783
2215	90-91	-1.7785449410217815
2215	92-93	-4.042857742542484
2215	94-95	-2.1919628702209017
2215	96-97	-1.7309598020598571
2215	98-99	-3.312873592993622
2215	100	-3.343696208844591
2216	1	-0.7818537328863115
2216	2	-0.7386033870404027
2216	3	-0.9089994067077107
2216	4	-0.9107779531234712
2216	5	-1.113470883682453
2216	6	-0.9831254026798462
2216	7	-1.0064054230288448
2216	8	-0.9316708445027189
2216	9	-1.191698001268719
2216	10-11	-1.1836123747287246
2216	12-13	-1.3011157900476462
2216	14-15	-1.8197458666651727
2216	16-17	-1.5131089771956212
2216	18-19	-3.5085119200861072
2216	20-21	-1.6708550129808373
2216	22-23	-1.1623899310840144
2216	24-25	-1.3972520924636669
2216	26-27	-1.3530826009538117
2216	28-29	-1.2878617227681275
2216	30-31	-1.3845209914647114
2216	32-33	-1.397774685865457
2216	34-35	-1.6680687246711798
2216	36-37	-1.6376803730940352
2216	38-39	-1.7284666815855019
2216	40-41	-1.7987061680944265
2216	42-43	-1.7118093196385118
2216	44-45	-1.609153849082091
2216	46-47	-1.7978337637963833
2216	48-49	-1.709053186689907
2216	50-51	-1.5663925526786215
2216	52-53	-1.616108268839902
2216	54-55	-1.4761534906964329
2216	56-57	-1.559432351255019
2216	58-59	-1.5952219021218426
2216	60-61	-1.5970097650315864
2216	62-63	-1.605541219829334
2216	64-65	-1.5937999887153609
2216	66-67	-1.6391522569211503
2216	68-69	-1.67842536470161
2216	70-71	-1.7256581910422923
2216	72-73	-1.6672053080246272
2216	74-75	-1.7249070204122816
2216	76-77	-1.6331527886288697
2216	78-79	-1.6770002412797425
2216	80-81	-1.6660844771194263
2216	82-83	-1.7926978799703406
2216	84-85	-1.6960446600707186
2216	86-87	-1.6319748648947474
2216	88-89	-1.481634231912217
2216	90-91	-1.55767659235012
2216	92-93	-1.5061353866479905
2216	94-95	-1.5037077918073862
2216	96-97	-1.417476574531527
2216	98-99	-1.4208228337804307
2216	100	-1.4162957946871089
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	20508.0
1	3593.0
2	532.0
3	498.0
4	518.0
5	261.0
6	267.0
7	352.0
8	612.0
9	1060.0
10	1833.0
11	3151.0
12	5274.0
13	7479.0
14	9929.0
15	12274.0
16	15182.0
17	18215.0
18	21091.0
19	24198.0
20	28080.0
21	32199.0
22	37392.0
23	43525.0
24	49469.0
25	55428.0
26	62943.0
27	73051.0
28	86817.0
29	104293.0
30	124150.0
31	149303.0
32	186844.0
33	237842.0
34	331643.0
35	451696.0
36	674246.0
37	1045235.0
38	843069.0
39	284854.0
40	354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.441464360139555	21.16456388963011	12.423673734243176	23.97029801598716
2	15.388704435262547	19.75179034078396	41.02314964298625	23.83635558096725
3	18.56759195963299	26.86824301801331	28.79437281774047	25.76979220461323
4	12.041225166485331	16.78813642285961	40.449528301511364	30.7211101091437
5	13.063529705690108	38.65680209958216	35.07753487667103	13.202133318056699
6	29.526250188316588	38.713094674934226	18.404874399551186	13.355780737198003
7	26.80196928789868	31.638364887534664	23.98689238025553	17.572773444311128
8	25.909277926147862	35.30149891049671	20.049647192819688	18.73957597053574
9	26.030485144036593	15.09931581026157	21.789058219832583	37.081140825869255
10-11	25.714152313468823	25.773276750149034	27.61530310457549	20.89726783180666
12-13	25.92241176775098	23.58862248981591	27.60096485045415	22.88800089197896
14-15	23.333042651227863	24.74943102959801	25.044562960449912	26.872963358724217
16-17	22.238220419114928	27.31409886148986	26.374734914755592	24.072945804639616
18-19	21.621623235983137	28.10773389910893	27.971317391427274	22.299325473480664
20-21	23.464266550656195	27.25026306436889	26.74431337959815	22.541157005376768
22-23	23.91666059631185	26.889114321300493	26.49815361699604	22.69607146539161
24-25	23.327833284305747	26.95760681667615	27.246327037394856	22.468232861623246
26-27	23.208052941808184	27.171522528843006	26.20775167010719	23.41267285924162
28-29	22.304974319533073	27.688769920514524	26.888659715623202	23.1175960443292
30-31	23.207411273860473	27.25417089200532	26.768779515160055	22.76963831897415
32-33	23.612124074061725	27.487674147368335	25.76739512735263	23.132806651217315
34-35	23.537678705095317	27.291467541319932	26.792820009136907	22.378033744447844
36-37	23.26150473635411	26.909268071588567	26.349504459150737	23.479722732906588
38-39	23.35107640705495	26.94944742264141	27.07106870118809	22.62840746911555
40-41	22.952100941573086	26.052720924189277	27.73060675061207	23.26457138362557
42-43	22.91775934968286	26.30633116967162	27.073638571720426	23.702270908925094
44-45	23.13159141733327	27.31944058191716	26.799659745781153	22.749308254968422
46-47	23.031494847773075	27.050002837732485	26.29867084593303	23.619831468561408
48-49	23.755436372578874	26.578413219761	26.666318932597093	22.999831475063036
50-51	23.278913851063106	26.43659050447999	26.684707821499693	23.599787822957214
52-53	22.785497523403134	27.075182703522344	26.461687908576764	23.67763186449776
54-55	22.76059318616844	26.945221056966044	26.468066474093753	23.82611928277176
56-57	23.902045498685435	26.360653668435912	26.148419778327074	23.588881054551575
58-59	22.73602296403865	26.80047422961829	27.125573784886154	23.33792902145691
60-61	22.791320095230244	27.43178371155063	26.411431213110525	23.3654649801086
62-63	23.26559446725434	26.611835324010492	25.97441250461996	24.14815770411521
64-65	22.366861761138424	26.89068285188881	26.487041176906274	24.25541421006649
66-67	23.227459049032852	26.485886171920793	26.376062399653406	23.910592379392952
68-69	22.56687874179577	26.660190603754465	26.676908484488767	24.096022169961003
70-71	22.532045726803364	26.27878520470369	26.838956366123046	24.350212702369898
72-73	23.19015079803449	26.414107403627163	27.217338807147605	23.178402991190737
74-75	23.378801585848542	26.728325722769597	26.70385484087904	23.18901785050282
76-77	23.01100488537515	26.740694862442417	26.256656694441176	23.991643557741256
78-79	22.91522533039709	26.72010414662736	26.35968544117934	24.004985081796207
80-81	22.500455186231147	27.047389839208012	27.09299307868057	23.359161895880266
82-83	23.426380178163033	26.054098759174092	28.054505711366968	22.465015351295907
84-85	23.64400888924464	26.60163803108926	26.64551279715522	23.108840282510883
86-87	22.27888673473199	27.353787016485594	27.12529037677729	23.242035872005133
88-89	23.396154282879717	25.953083348569955	26.96141406013597	23.689348308414363
90-91	23.00578070563716	26.13947843391406	27.03818757952763	23.81655328092115
92-93	23.323888552926146	26.35624376744615	26.963489749576485	23.356377930051224
94-95	22.178935263640874	26.381659608575042	27.678699715790618	23.760705411993467
96-97	22.612574693706243	26.2917040277033	27.154338492393563	23.94138278619689
98-99	21.962593805319834	27.056118302376753	26.99835190568039	23.982935986623026
100	22.8209937116413	26.280101560386726	26.179169869377457	24.71973485859452
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17305.0
1	10158.0
2	2752.5
3	1926.0
4	985.5
5	513.5
6	404.0
7	371.0
8	346.5
9	341.5
10	342.5
11	336.5
12	314.5
13	322.5
14	364.5
15	471.5
16	551.5
17	619.5
18	813.5
19	1025.5
20	1288.5
21	1658.5
22	2286.5
23	3202.5
24	4288.5
25	5920.0
26	8496.0
27	11415.5
28	16038.0
29	25881.0
30	38992.0
31	51505.5
32	68459.0
33	84984.0
34	98496.5
35	113007.5
36	125974.0
37	149344.5
38	159117.0
39	148688.5
40	139108.0
41	129578.5
42	128898.0
43	138212.0
44	151011.0
45	180208.5
46	211403.5
47	232822.5
48	247179.5
49	242213.5
50	230015.5
51	232673.0
52	232932.5
53	222738.5
54	209748.5
55	180959.0
56	158858.5
57	133288.5
58	107022.5
59	101166.0
60	83774.0
61	58348.0
62	43385.0
63	35948.5
64	26564.0
65	13384.0
66	7262.5
67	4997.0
68	3609.5
69	2626.5
70	1971.5
71	1500.0
72	1182.5
73	995.0
74	801.5
75	619.5
76	476.5
77	328.5
78	207.0
79	133.5
80	99.5
81	81.0
82	52.5
83	26.5
84	27.0
85	27.5
86	26.0
87	17.5
88	13.0
89	13.0
90	11.5
91	10.0
92	7.0
93	8.0
94	7.5
95	4.5
96	3.0
97	1.0
98	1.5
99	2.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9902045052146254
2	0.4496302428474668
3	0.48254595722937615
4	0.46723678321179735
5	0.43519248365106966
6	0.4847641040469296
7	0.5013011807670827
8	0.5032618641147415
9	0.5167489889607586
10-11	0.5308104553934636
12-13	0.5291864550448977
14-15	0.5266316252282512
16-17	0.5311471383925566
18-19	0.5302559186890752
20-21	0.5292854794563956
22-23	0.491992886086278
24-25	0.5263642593172069
26-27	0.5061236696070315
28-29	0.5127385002950927
30-31	0.5148081104953993
32-33	0.5184819161619723
34-35	0.530265821130225
36-37	0.5481199225233004
38-39	0.5369598713474846
40-41	0.5415645064821379
42-43	0.5390492864300908
44-45	0.549001239785632
46-47	0.5475059711720134
48-49	0.5201653311574369
50-51	0.5355042124984651
52-53	0.5276416742255301
54-55	0.5365736761426426
56-57	0.5248194784978393
58-59	0.530741138305415
60-61	0.5245521125867949
62-63	0.5192939163362552
64-65	0.5295033331616911
66-67	0.5281566011653193
68-69	0.5484070933166444
70-71	0.5320878703017868
72-73	0.5356428466745622
74-75	0.534028748767146
76-77	0.5210466484197684
78-79	0.5521205087478165
80-81	0.5266217227871015
82-83	0.5259582592300655
84-85	0.5349496757940768
86-87	0.8397864241492813
88-89	1.105290676257511
90-91	1.0955961863718644
92-93	1.0956060888130144
94-95	1.100804870416655
96-97	1.0931898931724648
98-99	1.0894071606532443
100	1.0851689158411333
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5049260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.807293956016714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1730727768216	20.347451443700827
2	12.928842676168959	7.190322576146481
3	5.303630875779946	4.42438868391058
4	2.570386930407421	2.8590201981817054
5	1.5577312130727017	2.165814487318756
6	0.9293054552370286	1.550488198122359
7	0.6026320072696492	1.1730295761416074
8	0.4564991497438234	1.015520483807855
9	0.3419943244534952	0.8558943040230927
>10	1.6363784822436944	8.332534201680897
>50	0.18179304426700127	3.5929069807654495
>100	0.2326569967494274	14.506937568809725
>500	0.046456948240151015	9.04122977551449
>1k	0.03633902392635545	17.78878343979876
>5k	0.0017813247022723262	3.1456154951335935
>10k+	4.987709166362513E-4	2.0100625869439464
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	19325	0.3827293504394703	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	16348	0.32377021583360727	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	15533	0.3076292367594459	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	14039	0.2780407426038667	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	12686	0.2512447368525289	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	12036	0.23837156335779897	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	11482	0.22739965856382915	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9726	0.19262228524575878	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8389	0.16614315761121431	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	8295	0.16428149867505337	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7638	0.15126969100422635	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7527	0.1490713490689725	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6827	0.13520793145926333	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6812	0.13491085822476956	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6782	0.13431671175578203	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6512	0.12896939353489423	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6422	0.12718695412793163	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6230	0.1233844167264114	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5980	0.11843319615151528	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5977	0.11837378150461651	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5728	0.11344236581201997	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5709	0.11306607304832789	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5659	0.11207582893334865	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5631	0.11152129222896028	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5603	0.11096675552457191	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5520	0.1093229502937064	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5428	0.10750090112214464	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5403	0.10700577906465501	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5367	0.10629280330186998	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5237	0.103718168602924	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5218	0.10334187583923189	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5141	0.10181689990216389	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.941464689875348E-5
2	0.0	0.0	0.0	0.0	5.941464689875348E-5
3	0.0	0.0	0.0	0.0	5.941464689875348E-5
4	0.0	0.0	0.0	0.0	7.921952919833797E-5
5	0.0	0.0	0.0	0.0	7.921952919833797E-5
6	0.0	0.0	0.0	0.0	1.3863417609709145E-4
7	0.0	0.0	0.0	0.0	1.5843905839667594E-4
8	0.0	0.0	0.0	0.0	1.5843905839667594E-4
9	0.0	0.0	0.0	0.0	2.1785370529542943E-4
10-11	0.0	0.0	0.0	3.9609764599168985E-5	2.4756102874480616E-4
12-13	0.0	0.0	0.0	3.9609764599168985E-5	4.060000871414821E-4
14-15	0.0	0.0	0.0	3.9609764599168985E-5	5.248293809389891E-4
16-17	0.0	0.0	0.0	3.9609764599168985E-5	6.436586747364961E-4
18-19	0.0	0.0	0.0	3.9609764599168985E-5	6.931708804854572E-4
20-21	0.0	0.0	0.0	5.9414646898753475E-5	7.426830862344185E-4
22-23	0.0	0.0	0.0	1.1882929379750696E-4	7.921952919833798E-4
24-25	0.0	0.0	0.0	1.9804882299584493E-4	8.615123800319255E-4
26-27	0.0	0.0	0.0	4.3570741059085886E-4	8.8131726233151E-4
28-29	0.0	0.0	0.0	0.001287317349472992	8.912197034813022E-4
30-31	0.0	0.0	0.0	0.005852342719527217	9.20927026930679E-4
32-33	0.0	0.0	0.0	0.01666580845510035	9.506343503800557E-4
34-35	0.0	0.0	0.0	0.0335890803800953	0.001000146556129017
36-37	0.0	0.0	0.0	0.06433616015020023	0.0010100489972788092
38-39	0.0	0.0	0.0	0.11425436598630294	0.0010694636441775625
40-41	0.0	0.0	0.0	0.1701239389534308	0.0011090734087767316
42-43	0.0	0.0	0.0	0.2295286834110345	0.0011486831733759006
44-45	0.0	0.0	0.0	0.302143284362461	0.0011882929379750697
46-47	0.0	0.0	0.0	0.395186621405909	0.0012080978202746542
48-49	0.0	0.0	0.0	0.5073515723096058	0.0012774149083231998
50-51	0.0	0.0	0.0	0.6273196468393389	0.001287317349472992
52-53	0.0	0.0	0.0	0.7457033307851051	0.0013764393198211224
54-55	0.0	0.0	0.0	0.8787030178679648	0.0014358539667198758
56-57	0.0	0.0	0.0	1.0438856386876494	0.001485366172468837
58-59	0.0	0.0	0.0	1.2479155361379688	0.001524975937068006
60-61	0.0	0.0	0.0	1.457876995837014	0.0015447808193675904
62-63	0.0	0.0	0.0	1.680889872971485	0.0016636101131650976
64-65	0.0	0.0	0.0	1.9153400696339662	0.0016834149954646819
66-67	0.0	0.0	0.0	2.1874888597537065	0.0016933174366144741
68-69	0.0	0.0	0.0	2.494385315868068	0.0017131223189140587
70-71	0.0	0.0	0.0	2.8361086575062484	0.00176263452466302
72-73	0.0	0.0	0.0	3.200102589290312	0.0019408784653592805
74-75	0.0	0.0	0.0	3.5570261780934236	0.0019507809065090727
76-77	0.0	0.0	0.0	3.9642343630551804	0.0019804882299584493
78-79	0.0	0.0	0.0	4.408061775388869	0.002000293112258034
80-81	0.0	0.0	0.0	4.88943924456257	0.002039902876857203
82-83	0.0	0.0	0.0	5.386957692810432	0.0020993175237559564
84-85	0.0	0.0	0.0	5.870068089185346	0.0020993175237559564
86-87	0.0	0.0	0.0	6.392976396541275	0.0021884394941040866
88	0.0	0.0	0.0	6.815355121344514	0.0022775614644522167
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5325	0.0	38.381695	94
GTACTAG	6125	0.0	32.572506	1
TACCCCG	385	0.0	31.725819	5
TATTCTC	2785	0.0	30.02583	5
GCATCGT	6140	0.0	29.978529	8
TAGGCAT	7245	0.0	29.503477	5
CAACGCA	10080	0.0	28.802364	5
CTGTGCG	7780	0.0	28.247532	9
GTATCAA	11930	0.0	27.47649	1
TAGTACT	8040	0.0	26.879114	4
CTAGGCA	8310	0.0	26.740734	4
ACTGTGC	8555	0.0	26.291061	8
GTACATG	21035	0.0	25.96468	1
ATCAACG	11195	0.0	25.853128	3
TACATGG	21035	0.0	25.665592	2
ACATGGG	20250	0.0	25.522552	3
CTAACGC	2790	0.0	25.260592	3
AGGGCTA	5860	0.0	25.25301	5
TACTGTG	9235	0.0	25.067015	7
TCTAACG	2845	0.0	24.938393	2
>>END_MODULE