##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146430_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5049260
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.840741415573767	31.0	31.0	34.0	30.0	34.0
2	31.836362159999684	31.0	31.0	34.0	30.0	34.0
3	32.00190800236074	33.0	31.0	34.0	30.0	34.0
4	35.74758380435945	37.0	35.0	37.0	35.0	37.0
5	35.64776303854426	37.0	35.0	37.0	33.0	37.0
6	35.67867548908157	37.0	35.0	37.0	33.0	37.0
7	35.609654087925755	37.0	35.0	37.0	33.0	37.0
8	35.59213468904354	37.0	35.0	37.0	33.0	37.0
9	37.194455623200234	39.0	37.0	39.0	33.0	39.0
10-11	37.1354175661384	39.0	37.0	39.0	33.0	39.0
12-13	37.06400254690786	39.0	37.0	39.0	33.0	39.0
14-15	38.2661696961535	40.0	38.0	41.0	33.0	41.0
16-17	38.26922984754202	40.0	38.0	41.0	33.0	41.0
18-19	38.323883895858	40.0	38.0	41.0	33.0	41.0
20-21	38.33549034908086	40.0	38.0	41.0	33.0	41.0
22-23	38.299956924381	40.0	38.0	41.0	33.0	41.0
24-25	38.22921735066129	40.0	37.0	41.0	33.0	41.0
26-27	38.08693254060991	40.0	37.0	41.0	33.0	41.0
28-29	38.021780221260144	40.0	37.0	41.0	33.0	41.0
30-31	37.90252423127349	40.0	37.0	41.0	33.0	41.0
32-33	37.78608043554897	40.0	36.5	41.0	33.0	41.0
34-35	37.69136160546298	40.0	36.0	41.0	32.0	41.0
36-37	37.61369596732987	40.0	36.0	41.0	32.0	41.0
38-39	37.494842115478306	40.0	36.0	41.0	32.0	41.0
40-41	37.373903106593836	40.0	36.0	41.0	31.0	41.0
42-43	37.24787661954425	39.5	36.0	41.0	31.0	41.0
44-45	37.04788850247363	39.0	35.0	41.0	31.0	41.0
46-47	36.81871165279665	39.0	35.0	41.0	30.5	41.0
48-49	36.557448616232875	39.0	35.0	41.0	30.0	41.0
50-51	36.35310926749662	39.0	35.0	41.0	30.0	41.0
52-53	36.15694903807687	38.0	35.0	40.5	29.0	41.0
54-55	35.948003469815376	38.0	35.0	40.0	29.0	41.0
56-57	35.68312792369575	37.5	34.0	40.0	28.5	41.0
58-59	35.395832359593285	37.0	34.0	40.0	28.0	41.0
60-61	35.12782645377739	36.5	34.0	40.0	28.0	41.0
62-63	34.852546907863726	36.0	34.0	39.5	28.0	41.0
64-65	34.57362534311959	35.5	33.5	39.0	27.0	41.0
66-67	34.34886359585364	35.0	33.0	39.0	27.0	41.0
68-69	34.15615476327224	35.0	33.0	38.5	27.0	41.0
70-71	33.952222206818426	35.0	33.0	37.5	27.0	40.0
72-73	33.75487883373009	35.0	33.0	37.0	26.5	40.0
74-75	33.579172888700526	35.0	33.0	36.5	26.5	39.5
76-77	33.427661479107826	35.0	33.0	36.0	27.0	39.0
78-79	33.3175511857183	35.0	33.0	36.0	27.0	39.0
80-81	33.174165224211066	35.0	33.0	36.0	27.0	38.0
82-83	32.98709206893683	35.0	33.0	35.0	27.0	37.0
84-85	32.85040639618479	35.0	33.0	35.0	27.0	37.0
86-87	32.72110596404225	35.0	33.0	35.0	27.0	36.0
88-89	32.616145435172676	35.0	33.0	35.0	27.0	36.0
90-91	32.52837821779825	35.0	33.0	35.0	27.0	36.0
92-93	32.45268217520983	35.0	33.0	35.0	26.0	36.0
94-95	32.41141117708337	35.0	33.0	35.0	26.5	35.0
96-97	32.35864067209849	35.0	33.0	35.0	26.0	35.0
98-99	32.31924806803373	35.0	33.0	35.0	26.0	35.0
100	31.820056206255966	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4414912910146924
1101	2	0.5667109684765848
1101	3	0.447228280960033
1101	4	0.3216608585697145
1101	5	0.3760355598476721
1101	6	0.3832885639268113
1101	7	0.3843299140747263
1101	8	0.3744603886591662
1101	9	0.4968911127344384
1101	10-11	0.5025824396611327
1101	12-13	0.513702960427473
1101	14-15	0.6377643186186432
1101	16-17	0.63692187557578
1101	18-19	0.6071854701222961
1101	20-21	0.5935223104190541
1101	22-23	0.5981017979835102
1101	24-25	0.6956864884105869
1101	26-27	0.6025732221941098
1101	28-29	0.6886560894500988
1101	30-31	0.7048438535551469
1101	32-33	0.7008658714055827
1101	34-35	0.7147887737757586
1101	36-37	0.7296388084250651
1101	38-39	0.7370716278934069
1101	40-41	0.7592928471522811
1101	42-43	0.7019809211299446
1101	44-45	0.6860750965477322
1101	46-47	0.7376855072711308
1101	48-49	0.7436945040430913
1101	50-51	0.7149128661522184
1101	52-53	0.7314453777035155
1101	54-55	0.7191046093505804
1101	56-57	0.7093604589624434
1101	58-59	0.6920292027753803
1101	60-61	0.7203666957214665
1101	62-63	0.7104074548414872
1101	64-65	0.696630621827488
1101	66-67	0.7297846123460161
1101	68-69	0.6510195374609822
1101	70-71	0.6834555743586606
1101	72-73	0.6468339573688269
1101	74-75	0.6414482812181532
1101	76-77	0.6274855998851194
1101	78-79	0.6379438625787728
1101	80-81	0.5718265729744942
1101	82-83	0.5748438787160381
1101	84-85	0.5682176675638004
1101	86-87	0.5876073482039033
1101	88-89	0.5509774421357747
1101	90-91	0.5772494606992211
1101	92-93	0.6304672917858127
1101	94-95	0.5640581892499625
1101	96-97	0.5590370485994285
1101	98-99	0.554477966896556
1101	100	0.6428662845367725
1102	1	0.3812105323729753
1102	2	0.4803603986309213
1102	3	0.37410706792357473
1102	4	0.2619266091091603
1102	5	0.27974396613706176
1102	6	0.30952167209411385
1102	7	0.28308222582140985
1102	8	0.3180280718797448
1102	9	0.396057757569217
1102	10-11	0.38754473501637676
1102	12-13	0.41434506824985107
1102	14-15	0.5719129909422378
1102	16-17	0.6140067110681926
1102	18-19	0.5741404957089884
1102	20-21	0.5702177595799824
1102	22-23	0.5263571703384713
1102	24-25	0.5543331844337729
1102	26-27	0.5907511878503584
1102	28-29	0.5650086667170413
1102	30-31	0.5888744918433702
1102	32-33	0.5922661820803086
1102	34-35	0.5703388681145469
1102	36-37	0.5774285007589626
1102	38-39	0.6079043389483942
1102	40-41	0.5568891979507384
1102	42-43	0.646016268431346
1102	44-45	0.6055251065014033
1102	46-47	0.582216836142301
1102	48-49	0.6162595045278465
1102	50-51	0.6239262461240287
1102	52-53	0.5687818533825961
1102	54-55	0.5883323261816997
1102	56-57	0.5884195507369654
1102	58-59	0.572113622933486
1102	60-61	0.5508904708148634
1102	62-63	0.5083506823644157
1102	64-65	0.5160540997791472
1102	66-67	0.5344405531866627
1102	68-69	0.5961446158263399
1102	70-71	0.5550603583289302
1102	72-73	0.5057568698884722
1102	74-75	0.5116265695969915
1102	76-77	0.4623255309820067
1102	78-79	0.42414554450746067
1102	80-81	0.4348179177014586
1102	82-83	0.4312645731436362
1102	84-85	0.46735643710966457
1102	86-87	0.5387605641745594
1102	88-89	0.4677399688701058
1102	90-91	0.4610782805607698
1102	92-93	0.42968066798640336
1102	94-95	0.4316684301355096
1102	96-97	0.4681727177390229
1102	98-99	0.4746608858606507
1102	100	0.5214201168842223
1103	1	0.2726224150951353
1103	2	-0.1822062815153167
1103	3	0.15242414571292073
1103	4	0.14674400515774266
1103	5	0.20661365855401215
1103	6	0.22101451517618642
1103	7	0.29934558866285954
1103	8	0.31569804185402006
1103	9	0.39582710680309674
1103	10-11	0.4032660142750757
1103	12-13	0.4270406312147159
1103	14-15	0.4606625367796582
1103	16-17	0.49218533918072893
1103	18-19	0.48593029630644935
1103	20-21	0.5259418102008979
1103	22-23	0.49200237859923135
1103	24-25	0.5058571112513874
1103	26-27	0.5342321931626515
1103	28-29	0.5813887037764474
1103	30-31	0.5233938381492891
1103	32-33	0.4994328675429358
1103	34-35	0.5010779072519469
1103	36-37	0.5124467679311806
1103	38-39	0.5498893383252934
1103	40-41	0.5701470112248757
1103	42-43	0.5667200746854917
1103	44-45	0.6310919428493094
1103	46-47	0.6037420038880228
1103	48-49	0.5656386687265851
1103	50-51	0.5543341298468292
1103	52-53	0.5421595537335975
1103	54-55	0.5317739027472896
1103	56-57	0.5481474367863441
1103	58-59	0.5123945503686826
1103	60-61	0.503671281460683
1103	62-63	0.48137929766630805
1103	64-65	0.4662440782385815
1103	66-67	0.44667310061201704
1103	68-69	0.3977735741139199
1103	70-71	0.3924797457052307
1103	72-73	0.36780361366287906
1103	74-75	0.37754244625215705
1103	76-77	0.36866110386376505
1103	78-79	0.3509631093906904
1103	80-81	0.3334468072971575
1103	82-83	0.348573329082285
1103	84-85	0.30895972937218374
1103	86-87	0.30473287476556976
1103	88-89	0.3054981111941615
1103	90-91	0.355316136868673
1103	92-93	0.30606110180065826
1103	94-95	0.3586423611707019
1103	96-97	0.39424393279976044
1103	98-99	0.38206096910678156
1103	100	0.332822864375359
1104	1	0.3543481387617575
1104	2	0.44195137999668077
1104	3	0.38540890170747133
1104	4	0.24409966011725004
1104	5	0.2700902755335477
1104	6	0.2832371023277247
1104	7	0.27440504900574325
1104	8	0.2569169651326959
1104	9	0.3264003959153783
1104	10-11	0.33883381504252696
1104	12-13	0.3336256235577437
1104	14-15	0.47018535852487986
1104	16-17	0.49585572707875514
1104	18-19	0.4977683130011883
1104	20-21	0.4465361268371453
1104	22-23	0.45473506359809335
1104	24-25	0.4358377612673223
1104	26-27	0.5242401578759086
1104	28-29	0.44884268797954974
1104	30-31	0.48049730294360415
1104	32-33	0.5066793881208937
1104	34-35	0.5313152038485924
1104	36-37	0.5486324560145732
1104	38-39	0.5086718162704287
1104	40-41	0.5337523509090261
1104	42-43	0.5685110875241008
1104	44-45	0.5046366344242301
1104	46-47	0.47235288168008793
1104	48-49	0.4265148238522727
1104	50-51	0.5077014670842033
1104	52-53	0.44542829457817135
1104	54-55	0.4308744283764554
1104	56-57	0.4044311136290375
1104	58-59	0.4173571023350888
1104	60-61	0.45853406498260085
1104	62-63	0.4781196189420456
1104	64-65	0.47075798322408247
1104	66-67	0.40350208268709054
1104	68-69	0.447664802479089
1104	70-71	0.44870388363175095
1104	72-73	0.44114330750464603
1104	74-75	0.36545207831698434
1104	76-77	0.33821241235798283
1104	78-79	0.3669830916278869
1104	80-81	0.40201985204647883
1104	82-83	0.3582755271179039
1104	84-85	0.37743223303959184
1104	86-87	0.36745089929668495
1104	88-89	0.3307329405892645
1104	90-91	0.35986742964823293
1104	92-93	0.32357099745269835
1104	94-95	0.31731333051114063
1104	96-97	0.36150188317338916
1104	98-99	0.3569694705018591
1104	100	0.4133011059887721
1105	1	0.09078654095099026
1105	2	-0.5028443976745152
1105	3	-0.005862154954087373
1105	4	0.01676927595414668
1105	5	0.08691344948670832
1105	6	0.16309554377971835
1105	7	0.19705242839125248
1105	8	0.17870450801293458
1105	9	0.26532522291335425
1105	10-11	0.26809410406769985
1105	12-13	0.2365265877594922
1105	14-15	0.31236508667208085
1105	16-17	0.3677922298368088
1105	18-19	0.3439021123261341
1105	20-21	0.2889627688651757
1105	22-23	0.277185926315596
1105	24-25	0.2872513990290386
1105	26-27	0.31985024413548757
1105	28-29	0.37725642961957817
1105	30-31	0.39042496549490835
1105	32-33	0.4020084957169132
1105	34-35	0.3882633036464327
1105	36-37	0.33998829929140584
1105	38-39	0.4110153475325191
1105	40-41	0.3791916669027984
1105	42-43	0.3321396381538264
1105	44-45	0.33186082735872446
1105	46-47	0.33569801899829343
1105	48-49	0.39050374295784707
1105	50-51	0.4226785895088554
1105	52-53	0.3265413912799531
1105	54-55	0.33002765781525056
1105	56-57	0.35886535825110144
1105	58-59	0.36070182982088284
1105	60-61	0.3181665670459708
1105	62-63	0.36021682063883276
1105	64-65	0.36974170029851194
1105	66-67	0.32565908339557126
1105	68-69	0.3704450730658593
1105	70-71	0.32277273222991454
1105	72-73	0.251325471922911
1105	74-75	0.2620269141202627
1105	76-77	0.2753244410608673
1105	78-79	0.22458499584721636
1105	80-81	0.22295423341460463
1105	82-83	0.2260291017897771
1105	84-85	0.17660502081700713
1105	86-87	0.20609186663100587
1105	88-89	0.24970998672828415
1105	90-91	0.2496038465443604
1105	92-93	0.26867546819674715
1105	94-95	0.2332841950789728
1105	96-97	0.25465908189990927
1105	98-99	0.27379722970868414
1105	100	0.2783063688861134
1106	1	0.3000736693604722
1106	2	0.3340144174349433
1106	3	0.26022457908710805
1106	4	0.1920345806902901
1106	5	0.19367217903401013
1106	6	0.20206574982921666
1106	7	0.1707632826509098
1106	8	0.23968173489177502
1106	9	0.2559250285222987
1106	10-11	0.2611408224594385
1106	12-13	0.2554914435468092
1106	14-15	0.311574131557407
1106	16-17	0.30091785917225167
1106	18-19	0.3355289808248756
1106	20-21	0.33338334265706493
1106	22-23	0.30685283181873046
1106	24-25	0.29758314375753514
1106	26-27	0.33463116540697513
1106	28-29	0.32746378956552036
1106	30-31	0.36716260414869595
1106	32-33	0.34398307363295544
1106	34-35	0.34427455743346513
1106	36-37	0.30145013267548393
1106	38-39	0.2810471016639795
1106	40-41	0.3449600478024166
1106	42-43	0.33155224146517526
1106	44-45	0.3901571861229485
1106	46-47	0.3636934929693538
1106	48-49	0.2956224751192593
1106	50-51	0.32008606647991655
1106	52-53	0.43211826845286083
1106	54-55	0.4053759434772104
1106	56-57	0.3593525561624702
1106	58-59	0.3188404941494909
1106	60-61	0.3475384946026594
1106	62-63	0.23338006620875262
1106	64-65	0.19307595155569146
1106	66-67	0.2807924877629304
1106	68-69	0.2865317325342076
1106	70-71	0.25952438956039714
1106	72-73	0.2426884373158984
1106	74-75	0.23272012763266758
1106	76-77	0.24228904655462458
1106	78-79	0.23584280811471103
1106	80-81	0.2741421058508493
1106	82-83	0.185649748744396
1106	84-85	0.24302830207749082
1106	86-87	0.21319560741473254
1106	88-89	0.204845823187064
1106	90-91	0.19987908472153038
1106	92-93	0.19848741109104395
1106	94-95	0.20113967481655237
1106	96-97	0.19214785784031818
1106	98-99	0.28314048604284636
1106	100	0.283782879492847
1107	1	0.06195927077423846
1107	2	-0.49382586356947655
1107	3	-0.06968376202829063
1107	4	-0.0222065510839613
1107	5	0.07050517269780698
1107	6	0.1204923869897101
1107	7	0.15358421969813207
1107	8	0.09278294631282336
1107	9	0.16012226909447946
1107	10-11	0.2127199582956365
1107	12-13	0.15355607399313698
1107	14-15	0.17818895552363045
1107	16-17	0.24152798981824475
1107	18-19	0.22971742590961242
1107	20-21	0.2321988249905047
1107	22-23	0.20524027824538393
1107	24-25	0.19889920600556366
1107	26-27	0.2583860983287849
1107	28-29	0.1520909527239951
1107	30-31	0.22650975270425988
1107	32-33	0.2854702446630881
1107	34-35	0.3194284653479329
1107	36-37	0.28324600336226524
1107	38-39	0.30346532346001
1107	40-41	0.3358659016912213
1107	42-43	0.3004418504006736
1107	44-45	0.2656176810982771
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2216	88-89	-0.572672851601979
2216	90-91	-0.6703274955212848
2216	92-93	-0.6792149417342657
2216	94-95	-0.6401483733561975
2216	96-97	-0.6461551524281219
2216	98-99	-0.5861733421124882
2216	100	-0.6020648247958533
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	12.0
13	47.0
14	279.0
15	847.0
16	2106.0
17	4205.0
18	7531.0
19	11824.0
20	16633.0
21	24119.0
22	33416.0
23	39522.0
24	41620.0
25	43737.0
26	49548.0
27	61327.0
28	77070.0
29	96718.0
30	119140.0
31	148261.0
32	184678.0
33	233264.0
34	319147.0
35	442036.0
36	663203.0
37	1130388.0
38	943574.0
39	354142.0
40	863.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09109893738374	21.60632590133814	13.547361959617533	24.755213201660588
2	14.944243867300742	20.181238377743416	40.1469902898392	24.72752746511664
3	18.0485544187373	26.531218930598634	29.598033740132063	25.822192910532003
4	12.265304242486677	17.28122681569337	39.13876460642272	31.31470433539724
5	12.667922726382383	38.7135350039997	35.14665814241404	13.471884127203873
6	29.455999492995012	39.081429754062974	17.783536597442	13.679034155500014
7	25.994353600099817	32.32028218136015	23.97319593958293	17.71216827895711
8	26.14270209892143	35.000217853705294	19.663713098553053	19.193366948820223
9	26.056550860918232	14.518266042944909	21.62485195850481	37.80033113763205
10-11	25.707687859211052	25.841925351437638	27.33132181745444	21.119064971896872
12-13	26.066433497185727	23.610875653065992	27.806074553498927	22.51661629624935
14-15	23.34292966355405	24.575716431908145	25.604286558898593	26.477067345639217
16-17	21.81650267403615	27.097899847279987	26.90937153466087	24.176225944022995
18-19	21.257471391847517	27.19949061842725	29.408546995005207	22.134490994720018
20-21	22.878080318589607	26.402808573027958	28.585229760155684	22.13388134822675
22-23	23.24789838909836	26.001292872446484	28.354081084174947	22.396727654280205
24-25	22.750741965232724	26.073803495344215	29.107327520005654	22.068127019417403
26-27	22.66577755789918	26.303099212140573	28.065122926152807	22.966000303807434
28-29	21.786638042010118	26.894931138424244	28.678766789588973	22.639664029976668
30-31	22.75553249387039	26.393936933332807	28.48858050486606	22.361950067930746
32-33	23.213837275165073	26.592827463826385	27.696187164059683	22.49714809694886
34-35	23.031130158219	26.521381306140306	28.480341231855792	21.967147303784902
36-37	22.841010365875395	26.094556430051135	28.070142951640438	22.994290252433032
38-39	22.914357747471907	26.049708274083727	28.85501043717297	22.180923541271394
40-41	22.437206341711892	25.34671581997752	29.373651776229114	22.842426062081472
42-43	22.63526299809931	25.511499897310852	28.481820653847656	23.37141645074218
44-45	22.850506806938046	26.508993397052244	28.072757196103982	22.56774259990573
46-47	22.8503384654385	26.3584564866931	27.369772996439085	23.42143205142932
48-49	23.750736870290925	26.107662233017276	27.416238903127045	22.725361993564757
50-51	23.11034915119732	26.078408130934843	27.186729063935015	23.62451365393282
52-53	22.50901122144631	26.66238221046252	27.062708198825174	23.76589836926599
54-55	22.631635912695366	26.57764599995702	26.860401452535577	23.930316634812034
56-57	23.741658547482892	25.970432670717496	26.645324246317102	23.64258453548251
58-59	22.504106798445985	26.452762930411737	27.638988724958036	23.40414154618424
60-61	22.67683054232753	27.05881362696081	26.85204054855617	23.412315282155486
62-63	23.150917164099294	26.190451670145727	26.426416940304126	24.232214225450857
64-65	22.241437359137777	26.473701096794382	26.89409933336766	24.39076221070018
66-67	22.940668533606907	26.117866776517744	26.80792829048217	24.13353639939318
68-69	22.440684256760964	25.99606887168854	27.23302060638877	24.330226265161727
70-71	22.364624996162767	25.675605019790204	27.371858793891725	24.5879111901553
72-73	22.991596788440287	25.699013320763836	27.690017943223367	23.619371947572514
74-75	23.06823709193924	26.167985806995418	27.133743480217255	23.630033620848096
76-77	22.769417397611956	26.13707854598209	26.619371226131328	24.474132830274627
78-79	22.538075600027014	26.195909832606194	26.864031374282536	24.401983193084252
80-81	22.14685991467176	26.634281956324607	27.52336287099627	23.695495258007355
82-83	23.004572749415967	25.565567910879267	28.472403427071548	22.957455912633222
84-85	23.32960845611828	26.084654271711898	26.971861002001212	23.613876270168603
86-87	21.83621022609109	26.796518018954814	27.49736078512929	23.86991096982481
88-89	22.80202941927081	25.559488147113584	27.31853803457769	24.319944399037915
90-91	22.35923544423637	25.5445444543365	27.498091185386254	24.598128916040878
92-93	22.762815003756344	25.872521113478896	27.155878370040814	24.208785512723942
94-95	21.552417064859057	25.77863516816571	27.94786233741516	24.721085429560077
96-97	21.924303325812712	25.68978669367924	27.4530543781237	24.93285560238435
98-99	21.28718861893634	26.37256026201591	27.3911556360178	24.949095483029947
100	22.108416551747652	25.591746208068933	26.543804908642603	25.756032331540812
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	111.0
1	81.5
2	77.0
3	103.0
4	77.0
5	71.0
6	149.5
7	353.5
8	628.0
9	768.0
10	626.5
11	461.0
12	490.5
13	586.0
14	743.0
15	1048.0
16	1428.5
17	1935.5
18	2660.5
19	3504.0
20	4450.0
21	5387.0
22	6554.5
23	7962.5
24	9174.0
25	10515.0
26	12755.0
27	15265.5
28	19302.5
29	28528.0
30	41076.0
31	52272.5
32	68367.5
33	84598.5
34	96386.5
35	110143.5
36	122896.0
37	146737.0
38	157367.0
39	146317.0
40	136311.5
41	126501.0
42	125348.5
43	133539.5
44	146286.5
45	177142.5
46	209094.5
47	230764.0
48	246922.0
49	242937.0
50	229354.0
51	230366.0
52	231387.0
53	222423.5
54	213268.5
55	185879.5
56	162420.0
57	136571.5
58	109098.0
59	105248.5
60	87204.5
61	59038.0
62	42879.5
63	35325.0
64	26016.0
65	12437.5
66	6594.5
67	4514.0
68	3167.5
69	2311.5
70	1801.0
71	1331.5
72	989.5
73	814.5
74	651.5
75	500.5
76	359.0
77	223.0
78	143.0
79	85.0
80	48.5
81	24.5
82	14.0
83	10.0
84	3.5
85	3.0
86	2.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01352673461061621
2	0.004337269223609004
3	0.0015843905839667595
4	0.027627810807920372
5	0.0027132688750430758
6	0.0
7	4.951220574896123E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009011221446310945
16-17	0.0029905372272372587
18-19	0.0
20-21	1.089268526477147E-4
22-23	7.525855273842108E-4
24-25	9.902441149792246E-6
26-27	1.7824394069626044E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.8521961633982006E-4
36-37	0.0
38-39	0.0
40-41	4.7531717519002785E-4
42-43	8.120001742829643E-4
44-45	0.0
46-47	0.0
48-49	6.634635570360806E-4
50-51	9.902441149792246E-6
52-53	0.0
54-55	4.0600008714148213E-4
56-57	8.912197034813022E-5
58-59	2.0795126414563717E-4
60-61	9.902441149792247E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0014556588490194603
70-71	8.912197034813022E-4
72-73	0.0
74-75	2.3765858759501393E-4
76-77	0.002921220139188713
78-79	5.743415866879503E-4
80-81	0.0033569275497795717
82-83	0.002168634611804502
84-85	0.006416781865065376
86-87	0.008120001742829642
88-89	0.008446782300772787
90-91	0.006238537924369116
92-93	0.009328099563104297
94-95	0.0017527320835132275
96-97	0.005020537662944669
98-99	0.002000293112258034
100	0.08720089676507053
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5049260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.72817383103498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.06606981358838	14.354950492429412
2	15.996214465175212	6.951370570756865
3	6.661330758671983	4.3421565801133495
4	3.4438328205830184	2.9931279268260527
5	1.9219912662510594	2.0880680167417025
6	1.2716035953340723	1.657777437815267
7	0.8021793739174135	1.2200925016103858
8	0.5903143646907842	1.026116250476667
9	0.42746110940212545	0.8359154361986799
>10	2.134947578310879	8.499201304809398
>50	0.24207960172192336	3.745323559696513
>100	0.3193619056349776	15.454068952871488
>500	0.06595941196238829	10.06217518332861
>1k	0.05327841851440817	20.795763332537607
>5k	0.0027369050606716526	3.9188585430523704
>10k+	6.386111808233855E-4	2.0550339107355855
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	21704	0.42984516543018186	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	16271	0.3222452398965393	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	15406	0.3051140167073987	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	13324	0.2638802517596638	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	13323	0.2638604468773642	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	12960	0.256671274602615	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	10683	0.21157555760646113	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9070	0.17963028245723134	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8756	0.17341154941516182	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8329	0.16495486467323925	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7947	0.15738939963479798	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7682	0.1521411058254081	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7528	0.14909115395127207	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7473	0.14800188542479492	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7413	0.14681359248681985	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7242	0.14342695761359092	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7097	0.14055524968015115	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7051	0.13964422509437024	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6855	0.1357624681636517	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6717	0.13302939440630904	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6578	0.1302765157666668	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6475	0.12823661288980961	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6350	0.12576100260236153	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6255	0.12387953878390101	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	6193	0.12265163608132677	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6083	0.12047309902837249	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6039	0.11960168420719075	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5886	0.11657153721535433	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5847	0.11579914680567054	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5817	0.115205000336683	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5676	0.11241251193244159	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5477	0.10847134035482428	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5285	0.10466880295330405	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5173	0.10245065613575059	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5160	0.10219319266585598	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	5145	0.10189611943136222	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	5097	0.10094548508098215	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9804882299584493E-5
2	0.0	0.0	0.0	1.9804882299584493E-5	1.9804882299584493E-5
3	0.0	0.0	0.0	1.9804882299584493E-5	1.9804882299584493E-5
4	0.0	0.0	0.0	5.941464689875348E-5	1.9804882299584493E-5
5	0.0	0.0	0.0	5.941464689875348E-5	1.9804882299584493E-5
6	0.0	0.0	0.0	5.941464689875348E-5	3.9609764599168985E-5
7	0.0	0.0	0.0	5.941464689875348E-5	3.9609764599168985E-5
8	0.0	0.0	0.0	5.941464689875348E-5	3.9609764599168985E-5
9	0.0	0.0	0.0	5.941464689875348E-5	5.941464689875348E-5
10-11	0.0	0.0	0.0	1.5843905839667594E-4	5.941464689875348E-5
12-13	0.0	0.0	0.0	1.5843905839667594E-4	1.3863417609709145E-4
14-15	0.0	0.0	0.0	1.5843905839667594E-4	2.277561464452217E-4
16-17	0.0	0.0	0.0	1.5843905839667594E-4	2.772683521941829E-4
18-19	0.0	0.0	0.0	1.5843905839667594E-4	2.970732344937674E-4
20-21	0.0	0.0	0.0	1.7824394069626044E-4	3.3668299909293635E-4
22-23	0.0	0.0	0.0	2.3765858759501393E-4	4.060000871414821E-4
24-25	0.0	0.0	0.0	3.2678055794314414E-4	4.951220574896123E-4
26-27	0.0	0.0	0.0	5.64439145538158E-4	5.64439145538158E-4
28-29	0.0	0.0	0.0	0.0014556588490194603	5.743415866879503E-4
30-31	0.0	0.0	0.0	0.0060701964248226475	5.842440278377426E-4
32-33	0.0	0.0	0.0	0.01714112563029038	5.941464689875348E-4
34-35	0.0	0.0	0.0	0.03420303173138242	6.535611158862883E-4
36-37	0.0	0.0	0.0	0.06542542867667737	6.535611158862883E-4
38-39	0.0	0.0	0.0	0.11562090286497427	6.931708804854572E-4
40-41	0.0	0.0	0.0	0.1721440369479884	7.22878203934834E-4
42-43	0.0	0.0	0.0	0.23249941575597216	7.426830862344185E-4
44-45	0.0	0.0	0.0	0.3064112364980215	7.624879685340031E-4
46-47	0.0	0.0	0.0	0.4007220860086429	7.822928508335875E-4
48-49	0.0	0.0	0.0	0.5140555249680151	7.921952919833797E-4
50-51	0.0	0.0	0.0	0.6360734048157553	7.921952919833797E-4
52-53	0.0	0.0	0.0	0.7563484550211318	8.120001742829643E-4
54-55	0.0	0.0	0.0	0.890942435129108	8.219026154327565E-4
56-57	0.0	0.0	0.0	1.0579867148849535	8.417074977323409E-4
58-59	0.0	0.0	0.0	1.2643238811231745	8.615123800319255E-4
60-61	0.0	0.0	0.0	1.4765926096101212	8.714148211817177E-4
62-63	0.0	0.0	0.0	1.7010710480347617	9.110245857808867E-4
64-65	0.0	0.0	0.0	1.9375611475741	9.308294680804712E-4
66-67	0.0	0.0	0.0	2.2124628163334825	9.506343503800557E-4
68-69	0.0	0.0	0.0	2.522607273144976	9.60536791529848E-4
70-71	0.0	0.0	0.0	2.867756859420984	0.0010100489972788092
72-73	0.0	0.0	0.0	3.2349591821375805	0.001128878291076316
74-75	0.0	0.0	0.0	3.595942771812107	0.0011486831733759006
76-77	0.0	0.0	0.0	4.010548080312759	0.0011486831733759006
78-79	0.0	0.0	0.0	4.463208470152062	0.0011486831733759006
80-81	0.0	0.0	0.0	4.953389209507928	0.0012180002614244462
82-83	0.0	0.0	0.0	5.460245659760044	0.0014358539667198758
84-85	0.0	0.0	0.0	5.9532683997259	0.0014655612901692525
86-87	0.0	0.0	0.0	6.484801733323299	0.0015150734959182136
88	0.0	0.0	0.0	6.914102264490242	0.001564585701667175
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5410	0.0	38.17002	94
GGTATCA	10770	0.0	37.23688	1
GTATCAA	17440	0.0	35.342407	1
TACCCCG	430	0.0	31.69549	5
GTACTAG	6005	0.0	31.395782	1
CAACGCA	19205	0.0	30.564411	5
ATCAACG	19755	0.0	29.720234	3
TATTCTC	2820	0.0	29.164656	5
TAGGCAT	6890	0.0	28.921083	5
TATCAAC	21775	0.0	28.151344	2
CTGTGCG	7810	0.0	27.925673	9
AACGCAG	22445	0.0	27.32464	6
CAGTACG	1095	0.0	27.043514	9
TAGTACT	8135	0.0	26.811615	4
AGGGCTA	5870	0.0	26.420666	5
GCTATTG	5855	0.0	26.086754	8
CTAGGCA	8085	0.0	26.047167	4
CTATTGA	5905	0.0	25.949823	9
ACTGTGC	8650	0.0	25.807213	8
GCATCGT	6675	0.0	25.698345	8
>>END_MODULE