FastQCFastQC Report
Mon 16 Jan 2017
ERR1146429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888255
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120300.3093932882488417No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC97980.25198964574082716No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT78590.2021215172358809No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA67180.17277673403621935No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC58530.15053025071658108No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA57650.148267024667878No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC51670.1328873749278275No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC45460.1169162001977751No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT39910.10264244500424997No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG39140.10066212221163477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG31550.034.235251
GTATCAA73400.033.8008081
CAACGCA70250.032.6331835
GGTATCA47250.031.5444241
GCATCGT32450.031.104098
ATCAACG73950.030.8150253
GGGCACC36600.029.28156594
TAGGCAT39900.028.9633065
TAGTACT41250.028.016144
AACGCAG85400.027.8671976
CTAGGCA44850.027.4432774
TATCAAC90550.027.1390552
CTGTGCG44600.026.4216949
GTACATG148300.025.7939031
ACGCAGA88750.025.4956917
TACATGG148800.025.451512
TCAACGC92550.025.3291854
CTAGTAC48150.025.2733293
CGCAGAG89900.024.9609998
ACATGGG148850.024.463123