##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146429_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3888255
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.27124687038273	31.0	30.0	34.0	26.0	34.0
2	30.381294951077024	31.0	30.0	34.0	26.0	34.0
3	30.591625034880686	31.0	30.0	34.0	26.0	34.0
4	34.04266052509416	37.0	35.0	37.0	28.0	37.0
5	34.25436577590719	37.0	35.0	37.0	30.0	37.0
6	34.38613979792992	37.0	35.0	37.0	30.0	37.0
7	34.23722672509905	37.0	35.0	37.0	30.0	37.0
8	34.185855094380386	37.0	35.0	37.0	30.0	37.0
9	35.538805453860405	39.0	35.0	39.0	29.0	39.0
10-11	35.4574689674417	39.0	35.0	39.0	28.5	39.0
12-13	35.447039224536454	39.0	35.0	39.0	28.5	39.0
14-15	36.44207993045724	40.0	36.0	41.0	27.5	41.0
16-17	36.264504642828214	39.5	36.0	41.0	27.0	41.0
18-19	36.21353640643425	39.5	35.0	41.0	27.0	41.0
20-21	36.54637800247154	40.0	36.0	41.0	28.0	41.0
22-23	36.27522526171766	39.5	35.5	41.0	27.0	41.0
24-25	36.38747587285299	39.0	35.0	41.0	27.5	41.0
26-27	36.46246786797677	39.0	35.0	41.0	28.5	41.0
28-29	36.29257803307654	39.0	35.0	41.0	27.5	41.0
30-31	36.23040181263832	39.0	35.0	41.0	27.5	41.0
32-33	36.324738089451444	39.0	35.0	41.0	28.5	41.0
34-35	36.36605173786184	39.0	35.0	41.0	29.0	41.0
36-37	36.29656118232987	39.0	35.0	41.0	28.0	41.0
38-39	36.129908146456444	39.0	35.0	41.0	28.0	41.0
40-41	36.10018723051857	39.0	35.0	41.0	28.0	41.0
42-43	35.93839575850863	39.0	35.0	41.0	27.0	41.0
44-45	35.775779494914815	39.0	34.0	41.0	27.0	41.0
46-47	35.65077033270708	38.0	34.0	40.5	27.0	41.0
48-49	35.532993335056474	38.0	34.0	40.0	27.0	41.0
50-51	35.330866416940246	38.0	33.5	40.0	26.0	41.0
52-53	35.162385826032505	38.0	33.0	40.0	26.0	41.0
54-55	34.96731734415567	38.0	33.0	40.0	26.0	41.0
56-57	34.84233300027904	37.0	33.0	40.0	26.0	41.0
58-59	34.60366925523146	37.0	33.0	40.0	26.0	41.0
60-61	34.34798707903674	36.0	33.0	40.0	26.0	41.0
62-63	34.08482134016416	36.0	32.0	39.0	25.5	41.0
64-65	33.798830323628465	35.0	32.0	39.0	25.0	41.0
66-67	33.5016906041399	35.0	32.0	39.0	25.0	40.5
68-69	33.209053804341536	35.0	31.5	38.0	24.0	40.0
70-71	33.1119527911621	35.0	31.5	37.5	25.0	40.0
72-73	32.88885939836765	35.0	31.0	37.0	24.5	40.0
74-75	32.62066132493882	35.0	31.0	36.5	24.0	39.0
76-77	32.37717176471193	35.0	31.0	36.0	24.0	39.0
78-79	32.15954714904244	35.0	31.0	36.0	24.0	39.0
80-81	31.92417511197182	35.0	31.0	35.0	23.5	37.0
82-83	31.70781751711243	34.5	31.0	35.0	23.0	37.0
84-85	31.526259208822466	34.0	31.0	35.0	23.0	36.5
86-87	31.361664422729476	34.0	30.5	35.0	23.0	36.0
88-89	31.352392525695976	34.5	31.0	35.0	23.0	36.0
90-91	31.284078976301707	34.0	31.0	35.0	23.0	36.0
92-93	31.155390014286613	34.0	31.0	35.0	22.5	35.5
94-95	31.125290007985587	34.0	31.0	35.0	23.0	35.0
96-97	31.039311850688804	34.0	31.0	35.0	21.5	35.0
98-99	30.95743566201291	34.0	30.5	35.0	20.5	35.0
100	30.463679979836712	34.0	29.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.748895742780153
1101	2	0.8369251376395077
1101	3	0.8575726866750024
1101	4	0.8381652673412816
1101	5	0.7209523904844559
1101	6	0.5344820556193497
1101	7	0.5863751661953458
1101	8	0.6594989741921751
1101	9	0.7465699250099149
1101	10-11	0.7810742772079493
1101	12-13	0.6913106498378525
1101	14-15	0.9710245353053182
1101	16-17	1.087331994291219
1101	18-19	1.1960572254416775
1101	20-21	0.8911210699364744
1101	22-23	1.175376369432385
1101	24-25	0.9797135987593606
1101	26-27	0.9436324847032438
1101	28-29	1.1510771931771515
1101	30-31	1.1242006565101477
1101	32-33	0.9854849432156882
1101	34-35	0.8866004055665613
1101	36-37	0.7517176290812699
1101	38-39	0.8933798349703963
1101	40-41	0.8757166025529344
1101	42-43	0.8736439362825479
1101	44-45	0.9031307951990328
1101	46-47	0.8173761132374935
1101	48-49	0.8625564946609501
1101	50-51	0.8443330420165296
1101	52-53	0.8910980361306287
1101	54-55	0.8905102985200273
1101	56-57	0.722592005073956
1101	58-59	0.6771510559537717
1101	60-61	0.693321406174455
1101	62-63	0.6124811147471689
1101	64-65	0.6505739340764478
1101	66-67	0.6745177155250488
1101	68-69	0.6750924135702974
1101	70-71	0.6495576836977222
1101	72-73	0.6621857429684397
1101	74-75	0.6885698543382617
1101	76-77	0.63606699651573
1101	78-79	0.6314883362401318
1101	80-81	0.7173529613405591
1101	82-83	0.662838063095414
1101	84-85	0.6471205742243562
1101	86-87	0.7535051714358545
1101	88-89	0.7410933654257512
1101	90-91	0.7734258524597131
1101	92-93	0.7442523203692417
1101	94-95	0.7115579738365696
1101	96-97	0.733062571321895
1101	98-99	0.7698444012231853
1101	100	0.8220724645047497
1102	1	0.6849379394711832
1102	2	0.8335216385334583
1102	3	0.8399558922623349
1102	4	0.8867789666817529
1102	5	0.7471902843229401
1102	6	0.5353595328924499
1102	7	0.6318719171797724
1102	8	0.6265396962676562
1102	9	0.7416278023197407
1102	10-11	0.7514139789359007
1102	12-13	0.6933200160772515
1102	14-15	0.9304681515163722
1102	16-17	1.186805886033909
1102	18-19	1.1403349879151392
1102	20-21	0.9120560194052345
1102	22-23	1.1633072083427862
1102	24-25	1.0189751026511544
1102	26-27	0.8867915919396552
1102	28-29	1.048100819435028
1102	30-31	1.1494419695121536
1102	32-33	0.9130526918072306
1102	34-35	0.7974257311433135
1102	36-37	0.8135581707459068
1102	38-39	0.8736039171167462
1102	40-41	0.8521620498800004
1102	42-43	0.9156708844765475
1102	44-45	0.8369945580073903
1102	46-47	0.8188825977648335
1102	48-49	0.7718075466073913
1102	50-51	0.915972214935465
1102	52-53	0.8401877446104749
1102	54-55	0.8011376583100116
1102	56-57	0.7289092202651943
1102	58-59	0.6835605021357054
1102	60-61	0.7568439818362762
1102	62-63	0.7757215959147601
1102	64-65	0.7114405812976372
1102	66-67	0.745303312906394
1102	68-69	0.8306565215359285
1102	70-71	0.6883398024988026
1102	72-73	0.6420280725369096
1102	74-75	0.666092365181818
1102	76-77	0.6397404366911701
1102	78-79	0.6325055778988329
1102	80-81	0.7416523839178453
1102	82-83	0.6779847414293627
1102	84-85	0.7014498942267302
1102	86-87	0.6715539525823786
1102	88-89	0.6350756646376681
1102	90-91	0.6306336436152584
1102	92-93	0.7449522226016896
1102	94-95	0.6936936546714314
1102	96-97	0.7453997829481338
1102	98-99	0.7157428904174381
1102	100	0.7613261361894317
1103	1	0.592060083590912
1103	2	0.7674555670154888
1103	3	0.7262804281998427
1103	4	0.6974767892561431
1103	5	0.7032467117693173
1103	6	0.5641244755644124
1103	7	0.5716388585346834
1103	8	0.6079624251383109
1103	9	0.7079289240138422
1103	10-11	0.8171993105945887
1103	12-13	0.6571270202149151
1103	14-15	0.8533410643285677
1103	16-17	1.0964055332798495
1103	18-19	1.106811950247149
1103	20-21	0.8169490516322782
1103	22-23	1.0620476749882144
1103	24-25	0.9849482818994062
1103	26-27	0.8197131817315793
1103	28-29	1.0679580481150523
1103	30-31	1.0450835423375437
1103	32-33	0.8443021519757892
1103	34-35	0.8346059514990714
1103	36-37	0.9144302770430528
1103	38-39	0.9804894568959313
1103	40-41	0.8750258027496969
1103	42-43	0.9787142712456287
1103	44-45	0.9662173052529397
1103	46-47	0.898549148350547
1103	48-49	0.8495603706479216
1103	50-51	0.9347924721070342
1103	52-53	0.9351076487529184
1103	54-55	0.9342062472992652
1103	56-57	0.8019680276461827
1103	58-59	0.87724066445098
1103	60-61	0.8113297267101416
1103	62-63	0.713452416838166
1103	64-65	0.6821177211069624
1103	66-67	0.8211909608477228
1103	68-69	0.8224778206853429
1103	70-71	0.7796466319648729
1103	72-73	0.7590693615966373
1103	74-75	0.689726020846102
1103	76-77	0.7240299112295574
1103	78-79	0.7150114493394497
1103	80-81	0.7374571440071236
1103	82-83	0.6712913541376437
1103	84-85	0.6508533976709252
1103	86-87	0.735676177023528
1103	88-89	0.6987817581174944
1103	90-91	0.6722519661409585
1103	92-93	0.8019179187545689
1103	94-95	0.7266901194287563
1103	96-97	0.7006680014685998
1103	98-99	0.7867391428181421
1103	100	0.8537489932442632
1104	1	0.5837062969424807
1104	2	0.7057549492528103
1104	3	0.6934299238760246
1104	4	0.7727324081033515
1104	5	0.6707442186637422
1104	6	0.4518921543770418
1104	7	0.46468135999098337
1104	8	0.5198900392463486
1104	9	0.6068991209113861
1104	10-11	0.6474548938516165
1104	12-13	0.5207148856535895
1104	14-15	0.7478683796225951
1104	16-17	0.8615034650271696
1104	18-19	0.8204596761535754
1104	20-21	0.7312795405656942
1104	22-23	0.9521739202374135
1104	24-25	0.8230697077422491
1104	26-27	0.7695907929123891
1104	28-29	0.9018302415331405
1104	30-31	0.9527224713458509
1104	32-33	0.7252503117678444
1104	34-35	0.6975600221295153
1104	36-37	0.7062434497008141
1104	38-39	0.8136173052480089
1104	40-41	0.6917531465268993
1104	42-43	0.7063697383738514
1104	44-45	0.7528546594097776
1104	46-47	0.6893780733302677
1104	48-49	0.6245199951114273
1104	50-51	0.7174282766290219
1104	52-53	0.8018689301073749
1104	54-55	0.7941293730723515
1104	56-57	0.7492858085695033
1104	58-59	0.7399620608615862
1104	60-61	0.6985891419530788
1104	62-63	0.5628986502636906
1104	64-65	0.5959692600840825
1104	66-67	0.6866306879477762
1104	68-69	0.6622054808012621
1104	70-71	0.5533489300252512
1104	72-73	0.5660567365751064
1104	74-75	0.5471083391724676
1104	76-77	0.5702833196785804
1104	78-79	0.47689444329977704
1104	80-81	0.5366787013460126
1104	82-83	0.5487289707678897
1104	84-85	0.576511591061756
1104	86-87	0.5679690633089471
1104	88-89	0.48419904519816725
1104	90-91	0.5305135273335218
1104	92-93	0.5319101016070675
1104	94-95	0.5318905503292193
1104	96-97	0.5643102315898183
1104	98-99	0.5621047435838875
1104	100	0.654782338810687
1105	1	0.5190792177400922
1105	2	0.6509053886654925
1105	3	0.5993708866683747
1105	4	0.6341339601824103
1105	5	0.6238393176129478
1105	6	0.3133898114265037
1105	7	0.3880278626240852
1105	8	0.42765137503840833
1105	9	0.43436642954628013
1105	10-11	0.5368005335939756
1105	12-13	0.5024619410470237
1105	14-15	0.6898677867770857
1105	16-17	0.7925332843779245
1105	18-19	0.82829260960807
1105	20-21	0.6572669840026748
1105	22-23	0.9086779619432903
1105	24-25	0.7033159959174924
1105	26-27	0.5297277862775758
1105	28-29	0.8330187854314985
1105	30-31	0.8370469236718421
1105	32-33	0.6723143103270317
1105	34-35	0.6043227418763948
1105	36-37	0.49914234640710475
1105	38-39	0.6921511898714598
1105	40-41	0.6018281905633032
1105	42-43	0.6588511006039752
1105	44-45	0.6659361458304431
1105	46-47	0.5734805869563004
1105	48-49	0.6074753083541751
1105	50-51	0.6942812215382759
1105	52-53	0.6945442449241312
1105	54-55	0.6562071463169445
1105	56-57	0.6146920947642727
1105	58-59	0.5078147482657371
1105	60-61	0.5943362483718744
1105	62-63	0.6046649082734348
1105	64-65	0.5801300204570978
1105	66-67	0.6505939726059395
1105	68-69	0.5891385960660429
1105	70-71	0.51800224620888
1105	72-73	0.4738845566830392
1105	74-75	0.4675084244886065
1105	76-77	0.511848659923686
1105	78-79	0.49283613904116663
1105	80-81	0.6123511226356086
1105	82-83	0.530447324395702
1105	84-85	0.5135565653771827
1105	86-87	0.5347518775349052
1105	88-89	0.5488319079955097
1105	90-91	0.5569186382153042
1105	92-93	0.5919551952699571
1105	94-95	0.5233104520794534
1105	96-97	0.5740988579503394
1105	98-99	0.5722185755431042
1105	100	0.6223393439765701
1106	1	0.40410938969013444
1106	2	0.4468157403171773
1106	3	0.4158085845354549
1106	4	0.5752380849671255
1106	5	0.4429300333951218
1106	6	0.1968153380060329
1106	7	0.22136479642878726
1106	8	0.32535022907191546
1106	9	0.439452730726984
1106	10-11	0.3541579401024393
1106	12-13	0.2812384966412651
1106	14-15	0.4744219340541562
1106	16-17	0.5565146030145627
1106	18-19	0.5208667327238601
1106	20-21	0.3726605769426996
1106	22-23	0.7122702369413716
1106	24-25	0.5047828808997963
1106	26-27	0.4272850041213445
1106	28-29	0.6383409280762393
1106	30-31	0.5527291367502798
1106	32-33	0.486287432596491
1106	34-35	0.47241465513298664
1106	36-37	0.39427692355504007
1106	38-39	0.520431844072867
1106	40-41	0.4631613307419329
1106	42-43	0.41406341828245274
1106	44-45	0.4907299023589431
1106	46-47	0.48049057762896297
1106	48-49	0.4748666122415841
1106	50-51	0.5543663218558805
1106	52-53	0.5117398949616998
1106	54-55	0.5367187796517214
1106	56-57	0.4539915679941302
1106	58-59	0.4239675535412317
1106	60-61	0.402086273575172
1106	62-63	0.3525305170792592
1106	64-65	0.402031751309579
1106	66-67	0.4355594737364825
1106	68-69	0.32574198338950566
1106	70-71	0.3376895283274166
1106	72-73	0.29801264959384355
1106	74-75	0.3626567151965858
1106	76-77	0.3835730252029421
1106	78-79	0.355221993509268
1106	80-81	0.36117345081052576
1106	82-83	0.3567753053106557
1106	84-85	0.3675918148390238
1106	86-87	0.3791024944015433
1106	88-89	0.4792843559348121
1106	90-91	0.4524647810091551
1106	92-93	0.4772322113009473
1106	94-95	0.4694209793494224
1106	96-97	0.49294492170325555
1106	98-99	0.5180597028379808
1106	100	0.43989710612689237
1107	1	0.36586390670061775
1107	2	0.5323805841733709
1107	3	0.5008770431433405
1107	4	0.6415815772072548
1107	5	0.6078801535057465
1107	6	0.26565711211168264
1107	7	0.20629888485205328
1107	8	0.331466756064728
1107	9	0.3948728574814808
1107	10-11	0.3830294274543036
1107	12-13	0.3102632187226888
1107	14-15	0.5088741826189889
1107	16-17	0.5990786853020893
1107	18-19	0.7580057339319524
1107	20-21	0.5251404865199163
1107	22-23	0.6644956180750441
1107	24-25	0.3922346715071612
1107	26-27	0.36844898147880656
1107	28-29	0.5793846995370444
1107	30-31	0.55943605413119
1107	32-33	0.48132572381171457
1107	34-35	0.32641554246529125
1107	36-37	0.3346622620561135
1107	38-39	0.45347279283879516
1107	40-41	0.29054471037914453
1107	42-43	0.36972316286795603
1107	44-45	0.4358685345333484
1107	46-47	0.3957206488569156
1107	48-49	0.39668649140686796
1107	50-51	0.40011778179380997
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2215	4	-2.114576314775732
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2215	10-11	-1.4317541689080713
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2215	22-23	-3.291725245747479
2215	24-25	-5.3578026903355145
2215	26-27	-3.551139347851702
2215	28-29	-1.8941071148760713
2215	30-31	-3.9547085377044695
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2215	100	-3.1708130454706698
2216	1	-0.629583668423443
2216	2	-0.5271131649185712
2216	3	-0.8100184090847407
2216	4	-0.7338528463815734
2216	5	-0.8716334338204632
2216	6	-0.801180758701598
2216	7	-0.9115088885742395
2216	8	-0.7674001719994905
2216	9	-0.8997114401462696
2216	10-11	-0.9651987205596271
2216	12-13	-1.0180574784442555
2216	14-15	-1.5606567408218481
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2216	18-19	-3.1658897320084805
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2216	24-25	-1.186719952460777
2216	26-27	-1.0779953740150745
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2216	32-33	-1.1104516855299735
2216	34-35	-1.380305568414208
2216	36-37	-1.3448850437973476
2216	38-39	-1.368098883413417
2216	40-41	-1.3872756090817475
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2216	54-55	-1.310286444222939
2216	56-57	-1.3097730540500123
2216	58-59	-1.4042310023568874
2216	60-61	-1.3643452973087236
2216	62-63	-1.3926427393389602
2216	64-65	-1.3994320904433408
2216	66-67	-1.3131755293147833
2216	68-69	-1.487789348534804
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2216	72-73	-1.5425620806737825
2216	74-75	-1.5315835031600855
2216	76-77	-1.3104108349248982
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2216	80-81	-1.5197615949033505
2216	82-83	-1.4864960601194035
2216	84-85	-1.458456624976396
2216	86-87	-1.4546945347014564
2216	88-89	-1.322358215843348
2216	90-91	-1.3522160099201734
2216	92-93	-1.3148939047670645
2216	94-95	-1.2728964222220576
2216	96-97	-1.2820328671125338
2216	98-99	-1.2419576330563302
2216	100	-1.2822996633891108
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16161.0
1	2656.0
2	415.0
3	419.0
4	376.0
5	207.0
6	190.0
7	278.0
8	504.0
9	880.0
10	1772.0
11	3290.0
12	5897.0
13	8265.0
14	10809.0
15	13359.0
16	15887.0
17	18798.0
18	21503.0
19	23971.0
20	27503.0
21	31374.0
22	35077.0
23	39963.0
24	44801.0
25	49521.0
26	55322.0
27	63065.0
28	73875.0
29	87747.0
30	102879.0
31	122792.0
32	149251.0
33	188187.0
34	251957.0
35	338645.0
36	494148.0
37	746702.0
38	647797.0
39	191773.0
40	239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.889398800234744	19.704680362657378	12.378869354651409	24.027051482456464
2	15.623479746967892	19.482929891710835	40.29320618861665	24.60038417270462
3	18.969158596392568	25.67851294689746	28.65015361777087	26.702174838939108
4	12.483387963880531	16.231926376024404	38.73594776139651	32.548737898698555
5	13.532752383277213	37.99945495570547	34.71743586365626	13.750356797361055
6	31.26378187599956	37.30886288991143	17.7310780593051	13.69627717478391
7	27.930434413969984	31.40248394606519	22.721390753367825	17.945690886597
8	26.33566425250961	35.172683705616045	19.79947425713422	18.692177784740117
9	26.186743387102446	15.396329167071709	20.75691797297549	37.660009472850355
10-11	25.73411133115769	25.73867516444668	27.491639652135056	21.035573852260573
12-13	25.932962404833	23.427540174152636	27.482693853150376	23.15680356786399
14-15	23.285918678914054	24.824375046137142	24.74237213240284	27.147334142545965
16-17	22.777602593197226	27.545920015762732	25.929024516661865	23.747452874378176
18-19	22.3703389013161	27.798391636364293	27.342440378041218	22.48882908427839
20-21	23.70336823804222	26.97857417306661	26.579373866510565	22.73868372238061
22-23	23.9643634082502	26.628836680095585	26.337512699060728	23.069287212593487
24-25	23.50673823294653	26.789004014679037	26.839048525981646	22.865209226392786
26-27	23.3817549829887	26.94681218754128	26.172107154776334	23.499325674693683
28-29	22.773502146203462	27.230433885080036	26.670418714878675	23.325645253837823
30-31	23.326542536414355	26.979469904187713	26.547215859440843	23.146771699957082
32-33	23.541912199377148	27.17555716453946	25.88012690720502	23.402403728878372
34-35	23.660859615894296	27.00740156564122	26.503073750622185	22.828665067842298
36-37	23.493802897071753	26.716068839429536	26.207840582047044	23.58228768145167
38-39	23.487237583283115	26.81743726105661	26.667688951220086	23.02763620444019
40-41	23.271002261855152	26.212551802663516	27.141990274785726	23.37445566069561
42-43	23.205510624955554	26.38067531661527	26.61908548450702	23.79472857392215
44-45	23.31966534771252	26.98951302143947	26.564783924276515	23.126037706571495
46-47	23.345467532611956	26.825800017947856	26.21663321486196	23.612099234578228
48-49	23.776978528868366	26.61522137402212	26.277509838931607	23.33029025817791
50-51	23.456654924728987	26.55622909901692	26.40332747852609	23.583788497728005
52-53	23.21572582991015	26.904002769750047	26.228902052154147	23.65136934818566
54-55	23.043922134684063	26.853676581059023	26.33259368230622	23.769807601950692
56-57	23.775013073344535	26.539425910496234	26.095300641676594	23.59026037448264
58-59	23.080002115148094	26.6735171952102	26.65859738530978	23.587883304331932
60-61	23.038320755043834	27.174715970424362	26.271465592868836	23.515497681662968
62-63	23.29243460055028	26.756946889819293	26.023864445482058	23.926754064148366
64-65	22.82198382533827	26.852281127897836	26.27494339230394	24.050791654459953
66-67	23.23643925979861	26.62495656095119	26.20209810605747	23.936506073192728
68-69	22.904023224903124	26.612562121585746	26.47018015179048	24.01323450172065
70-71	22.87557497872556	26.38601904110783	26.5544303712367	24.183975608929913
72-73	23.25073153929936	26.516450713024554	26.716247050828663	23.516570696847424
74-75	23.348761343944826	26.768225460729685	26.45746734201869	23.425545853306808
76-77	23.230439505562543	26.719409109518498	26.185551204376555	23.8646001805424
78-79	23.145174544918014	26.600136787424866	26.256655261679985	23.998033405977136
80-81	22.826409313329414	26.96150688739068	26.64907597137741	23.563007827902496
82-83	23.411904266948806	26.272614870043036	27.29946933334161	23.016011529666553
84-85	23.52306965890955	26.685787087652084	26.374816277431677	23.416326976006687
86-87	22.691647255956795	27.098029704870747	26.719741340402464	23.49058169876999
88-89	23.369163993314245	26.201519600748163	26.621947589418323	23.80736881651927
90-91	23.115671263343113	26.360031436252907	26.65668376658738	23.8676135338166
92-93	23.33526732517745	26.436372535200647	26.724065741506	23.504294398115906
94-95	22.61803101391505	26.386222603141857	27.112059162350437	23.883687220592652
96-97	22.911003933096787	26.313052035255357	26.768632278855627	24.00731175279223
98-99	22.482872466561147	26.839014903044202	26.617807693922973	24.060304936471677
100	23.022232068817296	26.309807338712382	26.168427222264263	24.49953337020606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13538.0
1	8027.0
2	2440.5
3	1896.0
4	1108.0
5	695.0
6	565.5
7	539.5
8	540.5
9	519.5
10	509.5
11	487.0
12	457.5
13	464.5
14	512.0
15	618.0
16	730.5
17	836.0
18	998.0
19	1243.0
20	1597.0
21	2146.5
22	2905.5
23	3970.0
24	5181.5
25	6761.0
26	9234.5
27	12210.0
28	16137.5
29	22871.5
30	31874.5
31	40663.0
32	51852.0
33	62043.5
34	70116.0
35	79219.0
36	88604.0
37	103247.5
38	110414.0
39	107322.0
40	104029.5
41	100486.0
42	101832.5
43	109399.0
44	118728.5
45	135741.5
46	152369.0
47	162565.0
48	169616.5
49	168773.0
50	165252.0
51	168755.5
52	170261.0
53	166043.0
54	160328.0
55	144732.5
56	129918.5
57	112296.5
58	94355.0
59	87128.5
60	74231.0
61	57303.0
62	45476.5
63	36537.5
64	26942.0
65	17133.5
66	12007.0
67	9084.0
68	6755.5
69	5155.0
70	4048.5
71	3155.0
72	2439.0
73	1914.0
74	1486.5
75	1122.5
76	836.5
77	559.0
78	327.5
79	212.0
80	148.0
81	104.0
82	68.0
83	48.0
84	40.5
85	29.5
86	21.0
87	17.0
88	15.5
89	12.5
90	11.0
91	11.5
92	9.0
93	6.5
94	7.5
95	8.5
96	5.0
97	2.5
98	3.5
99	4.0
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0030720721763362
2	0.45264520974061634
3	0.48636213417072693
4	0.47116251377545965
5	0.43747130782317517
6	0.48975697324378165
7	0.5075027229438398
8	0.5098431044260214
9	0.5236024900630232
10-11	0.5373104387443725
12-13	0.5360502333308901
14-15	0.5326168165410962
16-17	0.5376576381950258
18-19	0.5361273887643686
20-21	0.5342499398830581
22-23	0.498076900820548
24-25	0.5323210540460952
26-27	0.512929321765162
28-29	0.5181115950471356
30-31	0.5214421379256248
32-33	0.5243869036367214
34-35	0.5357158931191499
36-37	0.5526515107676837
38-39	0.542647022893303
40-41	0.5477135630250588
42-43	0.5447173603583098
44-45	0.5543617895431241
46-47	0.5528958363070323
48-49	0.5261229008899879
50-51	0.5416954392137346
52-53	0.5338641627156655
54-55	0.5430713777774349
56-57	0.5311508633050044
58-59	0.5378633860176352
60-61	0.5301092649530444
62-63	0.5248884139543317
64-65	0.536178825720021
66-67	0.5340956290161011
68-69	0.5540660270481231
70-71	0.5384934887243764
72-73	0.5418111723639525
74-75	0.5385063479632894
76-77	0.5263415079515105
78-79	0.5573579922098731
80-81	0.5318581214452242
82-83	0.5315237812334839
84-85	0.5403709376056869
86-87	0.8485811758745246
88-89	1.1152432132151826
90-91	1.1062288867371095
92-93	1.1062288867371095
94-95	1.1103824209060362
96-97	1.104325719377973
98-99	1.0998635634751321
100	1.095452844527944
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3888255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.00980959946108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.87296724196831	33.062916844226656
2	12.233076746230415	10.522846033419299
3	4.3899629938812295	5.664344175465354
4	2.081894631959141	3.581675669068111
5	1.1359095065087124	2.4427625798578756
6	0.6854686813136901	1.7689126481817283
7	0.44298959568883506	1.3337028715583328
8	0.3237591597150814	1.113985585234173
9	0.24480865197398002	0.9476256158731451
>10	1.3064025393095524	10.305304830676484
>50	0.12318174374515792	3.725349657110768
>100	0.12963581142024103	11.855728426145932
>500	0.020959887404402393	6.074022864105717
>1k	0.008563611133560821	6.230829000897973
>5k	3.593123552543002E-4	1.060141380654786
>10k+	5.9885392542383364E-5	0.30985181752373847
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12030	0.3093932882488417	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	9798	0.25198964574082716	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7859	0.2021215172358809	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	6718	0.17277673403621935	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	5853	0.15053025071658108	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5765	0.148267024667878	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5167	0.1328873749278275	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4546	0.1169162001977751	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	3991	0.10264244500424997	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	3914	0.10066212221163477	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.571847782617138E-5
2	0.0	0.0	0.0	0.0	2.571847782617138E-5
3	0.0	0.0	0.0	0.0	2.571847782617138E-5
4	0.0	0.0	0.0	0.0	2.571847782617138E-5
5	5.143695565234276E-5	0.0	0.0	0.0	2.571847782617138E-5
6	5.143695565234276E-5	0.0	0.0	0.0	5.143695565234276E-5
7	5.143695565234276E-5	0.0	0.0	0.0	5.143695565234276E-5
8	7.715543347851414E-5	0.0	0.0	0.0	5.143695565234276E-5
9	7.715543347851414E-5	0.0	0.0	0.0	5.143695565234276E-5
10-11	1.8002934478319966E-4	0.0	0.0	0.0	5.143695565234276E-5
12-13	2.0574782260937105E-4	0.0	0.0	0.0	7.715543347851414E-5
14-15	2.4432553934862814E-4	0.0	0.0	0.0	1.1573315021777122E-4
16-17	3.0862173391405656E-4	0.0	0.0	0.0	1.285923891308569E-4
18-19	3.343402117402279E-4	0.0	0.0	0.0	1.285923891308569E-4
20-21	3.343402117402279E-4	0.0	0.0	0.0	1.414516280439426E-4
22-23	3.343402117402279E-4	0.0	0.0	6.429619456542845E-5	1.9288858369628537E-4
24-25	3.986364063056564E-4	0.0	0.0	9.001467239159983E-5	2.0574782260937105E-4
26-27	4.243548841318278E-4	0.0	0.0	2.571847782617138E-4	2.829032560878852E-4
28-29	4.3721412304491346E-4	0.0	0.0	0.001285923891308569	2.829032560878852E-4
30-31	4.5007336195799916E-4	0.0	0.0	0.005413739582409076	2.829032560878852E-4
32-33	4.6293260087108487E-4	0.0	0.0	0.01586830081874774	2.829032560878852E-4
34-35	5.143695565234276E-4	0.0	0.0	0.03189091250445251	3.0862173391405656E-4
36-37	5.658065121757704E-4	0.0	0.0	0.061852939171942165	3.0862173391405656E-4
38-39	5.658065121757704E-4	0.0	0.0	0.11028083291862288	3.857771673925707E-4
40-41	5.915249900019417E-4	0.0	0.0	0.16404531081423412	3.857771673925707E-4
42-43	5.915249900019417E-4	0.0	0.0	0.2215261087557272	3.857771673925707E-4
44-45	6.043842289150275E-4	0.0	0.0	0.2900915706403001	3.857771673925707E-4
46-47	6.172434678281131E-4	0.0	0.0	0.3796818881477681	4.114956452187421E-4
48-49	6.172434678281131E-4	0.0	0.0	0.48512764723507074	4.3721412304491346E-4
50-51	6.172434678281131E-4	0.0	0.0	0.5969515888232639	4.3721412304491346E-4
52-53	6.686804234804558E-4	0.0	0.0	0.7095599439851552	4.6293260087108487E-4
54-55	6.686804234804558E-4	0.0	0.0	0.8361462918455709	4.886510786972562E-4
56-57	6.686804234804558E-4	0.0	0.0	0.9930290065852163	5.143695565234276E-4
58-59	6.686804234804558E-4	0.0	0.0	1.1805424284158317	5.40088034349599E-4
60-61	6.943989013066272E-4	0.0	0.0	1.3840784619321522	5.40088034349599E-4
62-63	6.943989013066272E-4	0.0	0.0	1.5984033968965514	5.915249900019417E-4
64-65	6.943989013066272E-4	0.0	0.0	1.8192093882731455	5.915249900019417E-4
66-67	6.943989013066272E-4	0.0	0.0	2.078323052371822	5.915249900019417E-4
68-69	7.972728126113128E-4	0.0	0.0	2.3773003571010647	5.915249900019417E-4
70-71	7.972728126113128E-4	0.0	0.0	2.7065997471873633	5.915249900019417E-4
72-73	7.972728126113128E-4	0.0	0.0	3.0477553555515264	6.172434678281131E-4
74-75	7.972728126113128E-4	0.0	0.0	3.3889624008713417	6.172434678281131E-4
76-77	8.487097682636555E-4	0.0	0.0	3.7658666934138836	6.301027067411988E-4
78-79	8.744282460898269E-4	0.0	0.0	4.191558938392673	6.686804234804558E-4
80-81	8.744282460898269E-4	0.0	0.0	4.654684427847453	6.686804234804558E-4
82-83	9.001467239159983E-4	0.0	0.0	5.141175154407311	6.686804234804558E-4
84-85	9.258652017421697E-4	1.285923891308569E-5	0.0	5.61100030733581	6.943989013066272E-4
86-87	9.387244406552554E-4	2.571847782617138E-5	0.0	6.114362355349636	7.072581402197129E-4
88	9.773021573945123E-4	2.571847782617138E-5	0.0	6.520945771303579	7.201173791327987E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	3155	0.0	34.23525	1
GTATCAA	7340	0.0	33.800808	1
CAACGCA	7025	0.0	32.633183	5
GGTATCA	4725	0.0	31.544424	1
GCATCGT	3245	0.0	31.10409	8
ATCAACG	7395	0.0	30.815025	3
GGGCACC	3660	0.0	29.281565	94
TAGGCAT	3990	0.0	28.963306	5
TAGTACT	4125	0.0	28.01614	4
AACGCAG	8540	0.0	27.867197	6
CTAGGCA	4485	0.0	27.443277	4
TATCAAC	9055	0.0	27.139055	2
CTGTGCG	4460	0.0	26.421694	9
GTACATG	14830	0.0	25.793903	1
ACGCAGA	8875	0.0	25.495691	7
TACATGG	14880	0.0	25.45151	2
TCAACGC	9255	0.0	25.329185	4
CTAGTAC	4815	0.0	25.273329	3
CGCAGAG	8990	0.0	24.960999	8
ACATGGG	14885	0.0	24.46312	3
>>END_MODULE