FastQCFastQC Report
Mon 16 Jan 2017
ERR1146429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888255
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC110540.28429205389049844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86120.22148753103898794No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT79930.20556779326458785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78950.20304738243762305No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA75700.19468887714411734No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA63440.16315802332923124No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC61460.1580657647196493No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC60710.15613687888268646No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC51370.1321158205930424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49680.1277693978404194No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG44070.11334133177993727No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT39840.10246241565946679No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74100.044.2300641
GTATCAA123350.038.5784151
CAACGCA146400.031.2040065
GTACTAG31100.030.844151
ATCAACG148600.030.7194923
TATCAAC160900.029.570242
GGGCACC37300.029.51095694
GTGGTAT25850.029.2865581
TAGTACT43100.028.467844
AACGCAG165050.028.3034426
CTGTGCG44350.027.6629879
TCAACGC167950.027.3467584
TAGGCAT39550.026.4998555
CTAGTAC47500.026.129073
ACGCAGA175400.025.9902537
CTAACGC13900.025.7046933
CGCAGAG178150.025.535648
GTACATG143900.025.4227091
CTAGGCA43250.025.2169844
TCTAACG14550.024.8804442
Produced by FastQC (version 0.11.5)