##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3888255
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.823827140966834	31.0	31.0	34.0	30.0	34.0
2	31.811780863137834	31.0	31.0	34.0	30.0	34.0
3	31.979056157582257	33.0	31.0	34.0	30.0	34.0
4	35.73019336437554	37.0	35.0	37.0	35.0	37.0
5	35.629886157157905	37.0	35.0	37.0	33.0	37.0
6	35.66097182412162	37.0	35.0	37.0	33.0	37.0
7	35.58882506419975	37.0	35.0	37.0	33.0	37.0
8	35.57132286848471	37.0	35.0	37.0	33.0	37.0
9	37.172647627277534	39.0	37.0	39.0	33.0	39.0
10-11	37.115598256801576	39.0	37.0	39.0	33.0	39.0
12-13	37.0536817929894	39.0	37.0	39.0	33.0	39.0
14-15	38.26572665630212	40.0	38.0	41.0	33.0	41.0
16-17	38.26201020766385	40.0	38.0	41.0	33.0	41.0
18-19	38.32033174264548	40.0	38.0	41.0	33.0	41.0
20-21	38.33912397720829	40.0	38.0	41.0	33.0	41.0
22-23	38.30605914478346	40.0	38.0	41.0	33.0	41.0
24-25	38.24445001678131	40.0	37.5	41.0	33.0	41.0
26-27	38.10773894716267	40.0	37.0	41.0	33.0	41.0
28-29	38.03525128881722	40.0	37.0	41.0	33.0	41.0
30-31	37.90692058519824	40.0	37.0	41.0	33.0	41.0
32-33	37.78255502789811	40.0	37.0	41.0	33.0	41.0
34-35	37.68878352885806	40.0	36.0	41.0	32.0	41.0
36-37	37.596462037597846	40.0	36.0	41.0	32.0	41.0
38-39	37.48990909804012	40.0	36.0	41.0	32.0	41.0
40-41	37.3688159598586	40.0	36.0	41.0	31.0	41.0
42-43	37.24657076246285	39.5	36.0	41.0	31.0	41.0
44-45	37.0674352633765	39.0	35.0	41.0	31.0	41.0
46-47	36.837346958982884	39.0	35.0	41.0	30.5	41.0
48-49	36.59832611287068	39.0	35.0	41.0	30.0	41.0
50-51	36.407325522631616	39.0	35.0	41.0	30.0	41.0
52-53	36.22667147602202	39.0	35.0	41.0	29.0	41.0
54-55	36.02813794362767	38.0	35.0	40.0	29.0	41.0
56-57	35.79532708117137	38.0	34.5	40.0	28.5	41.0
58-59	35.5383594954549	37.5	34.0	40.0	28.0	41.0
60-61	35.2801110009503	37.0	34.0	40.0	28.0	41.0
62-63	35.0108691430989	36.5	34.0	40.0	28.0	41.0
64-65	34.73454621674762	36.0	34.0	39.0	27.5	41.0
66-67	34.49989494001808	35.5	33.0	39.0	27.5	41.0
68-69	34.2964968861353	35.0	33.0	39.0	27.0	41.0
70-71	34.04544210706345	35.0	33.0	38.0	27.0	40.0
72-73	33.818641395690356	35.0	33.0	37.0	26.5	40.0
74-75	33.622188873929304	35.0	33.0	37.0	26.5	39.0
76-77	33.44276005560335	35.0	33.0	36.0	27.0	39.0
78-79	33.3153222975345	35.0	33.0	36.0	27.0	39.0
80-81	33.14786787389201	35.0	33.0	36.0	27.0	37.5
82-83	32.95798025078088	35.0	33.0	35.0	27.0	37.0
84-85	32.81050214042031	35.0	33.0	35.0	27.0	37.0
86-87	32.66947358133662	35.0	33.0	35.0	27.0	36.0
88-89	32.54960618580829	35.0	33.0	35.0	26.0	36.0
90-91	32.459458677478715	35.0	33.0	35.0	26.0	36.0
92-93	32.38365063505351	35.0	33.0	35.0	26.0	36.0
94-95	32.33579780647102	35.0	33.0	35.0	26.0	35.0
96-97	32.28066317666922	35.0	33.0	35.0	26.0	35.0
98-99	32.23499616151719	35.0	33.0	35.0	26.0	35.0
100	31.741046819202957	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4673014908539841
1101	2	0.5748514732747267
1101	3	0.4610131887220703
1101	4	0.31673389580797107
1101	5	0.34127684065826713
1101	6	0.36432182353421894
1101	7	0.37443797857179817
1101	8	0.37973987667801623
1101	9	0.480272263986528
1101	10-11	0.4589463753506067
1101	12-13	0.4578677556584907
1101	14-15	0.6134115237167066
1101	16-17	0.5928248902790756
1101	18-19	0.626634442184482
1101	20-21	0.5897342109010282
1101	22-23	0.617144860012921
1101	24-25	0.6442732924376244
1101	26-27	0.6658351374949376
1101	28-29	0.6663938568475274
1101	30-31	0.6958544189472065
1101	32-33	0.7116232276664078
1101	34-35	0.6988985078966223
1101	36-37	0.696072603557603
1101	38-39	0.6600495353308204
1101	40-41	0.6850944102964007
1101	42-43	0.7139998748899004
1101	44-45	0.7254105547599394
1101	46-47	0.7793223364425685
1101	48-49	0.6771702982892336
1101	50-51	0.659808413704468
1101	52-53	0.6921928456499131
1101	54-55	0.6241225937997825
1101	56-57	0.5804322255156151
1101	58-59	0.6147730924827783
1101	60-61	0.6276153771062027
1101	62-63	0.5774830422349808
1101	64-65	0.6141274236749084
1101	66-67	0.6089044361454654
1101	68-69	0.5958793222432135
1101	70-71	0.46026862750697006
1101	72-73	0.43989330293687345
1101	74-75	0.4662715237247568
1101	76-77	0.49407913509959656
1101	78-79	0.45181346298448943
1101	80-81	0.49310120838522664
1101	82-83	0.39543623998538635
1101	84-85	0.43353583709044585
1101	86-87	0.5408306530439191
1101	88-89	0.45460042527472666
1101	90-91	0.40775182867862725
1101	92-93	0.4824822387797525
1101	94-95	0.4442300846682983
1101	96-97	0.4292860399028271
1101	98-99	0.4520246437091018
1101	100	0.45730206977602705
1102	1	0.4193575802599767
1102	2	0.5417874058205214
1102	3	0.39928027238624964
1102	4	0.29730462063822927
1102	5	0.2997242811470002
1102	6	0.3346293862053784
1102	7	0.3440633289955457
1102	8	0.30603772741235247
1102	9	0.4325047130906867
1102	10-11	0.4520457957672761
1102	12-13	0.4263602718495534
1102	14-15	0.5288597420246219
1102	16-17	0.569232256874983
1102	18-19	0.5444475819272583
1102	20-21	0.5295224982403965
1102	22-23	0.5416468396707899
1102	24-25	0.5571799595364126
1102	26-27	0.6401931665875367
1102	28-29	0.6486167722650933
1102	30-31	0.6777884879678595
1102	32-33	0.667223795906537
1102	34-35	0.6660929032772458
1102	36-37	0.6232490118694614
1102	38-39	0.6576122334991581
1102	40-41	0.6034431109602494
1102	42-43	0.6455964838142876
1102	44-45	0.5817950189019285
1102	46-47	0.5468463597123261
1102	48-49	0.5344408821397693
1102	50-51	0.45119960274153925
1102	52-53	0.4621954923957645
1102	54-55	0.5305129996161426
1102	56-57	0.5790678010207913
1102	58-59	0.5598205718624598
1102	60-61	0.5425134503832538
1102	62-63	0.5233564143512481
1102	64-65	0.5288545387759385
1102	66-67	0.4875523541287521
1102	68-69	0.5252761612724512
1102	70-71	0.48894422829128104
1102	72-73	0.48113703041928346
1102	74-75	0.48694801035869517
1102	76-77	0.5333538727206601
1102	78-79	0.4656814659877355
1102	80-81	0.39188140964594
1102	82-83	0.4420540768918215
1102	84-85	0.4364507685181209
1102	86-87	0.4890497711914108
1102	88-89	0.46454260198012065
1102	90-91	0.4495791906292226
1102	92-93	0.469336824401978
1102	94-95	0.421034058027999
1102	96-97	0.41235753444998835
1102	98-99	0.424714682762108
1102	100	0.4562322983144327
1103	1	0.3044578472461694
1103	2	-0.1966800340223145
1103	3	0.25308098834715764
1103	4	0.19904996103324635
1103	5	0.2549266333542164
1103	6	0.29320358751616027
1103	7	0.3581692278334714
1103	8	0.3584280322871436
1103	9	0.3981275039078582
1103	10-11	0.4433896363523644
1103	12-13	0.4478250016697629
1103	14-15	0.5803123356087028
1103	16-17	0.5872262212155803
1103	18-19	0.566578388106727
1103	20-21	0.5457901847690181
1103	22-23	0.5408121433490152
1103	24-25	0.5072764552670037
1103	26-27	0.5615016547459106
1103	28-29	0.6068324842346868
1103	30-31	0.6201993793320426
1103	32-33	0.5786436585129024
1103	34-35	0.5711471559937351
1103	36-37	0.5578178371467217
1103	38-39	0.5297028927234422
1103	40-41	0.47620438525404296
1103	42-43	0.5423705198610094
1103	44-45	0.4807866962827063
1103	46-47	0.529054656804739
1103	48-49	0.6007579993490779
1103	50-51	0.5955300246985544
1103	52-53	0.5510842620862064
1103	54-55	0.524029479535514
1103	56-57	0.5624432480837527
1103	58-59	0.5605345109843327
1103	60-61	0.5612754470196606
1103	62-63	0.5154683147394863
1103	64-65	0.48582051000217064
1103	66-67	0.5032761931404295
1103	68-69	0.4585233553867596
1103	70-71	0.4843740771604743
1103	72-73	0.44578263692025644
1103	74-75	0.5246404035353365
1103	76-77	0.4614655161903869
1103	78-79	0.42663410275833513
1103	80-81	0.4546230959665962
1103	82-83	0.44483765413269793
1103	84-85	0.37486025040647064
1103	86-87	0.4801157739410442
1103	88-89	0.44985865025439153
1103	90-91	0.4459268354787227
1103	92-93	0.40783848336934625
1103	94-95	0.45263635478042374
1103	96-97	0.45881053239188674
1103	98-99	0.5186996951156502
1103	100	0.47132576548745675
1104	1	0.2972692939735566
1104	2	0.41194170526237883
1104	3	0.38898107960699235
1104	4	0.2656037544244967
1104	5	0.27036330632061834
1104	6	0.27733850816027683
1104	7	0.30164491138417304
1104	8	0.2935790634724711
1104	9	0.38812379535641384
1104	10-11	0.35310529347474073
1104	12-13	0.35579359502298047
1104	14-15	0.4613919997191118
1104	16-17	0.45095778543579
1104	18-19	0.5226703197919136
1104	20-21	0.49667217000995834
1104	22-23	0.5009640449762216
1104	24-25	0.470097785579199
1104	26-27	0.5224768343783524
1104	28-29	0.523194316911308
1104	30-31	0.5321384016086768
1104	32-33	0.5297691467673431
1104	34-35	0.5073409655214007
1104	36-37	0.5350294202975974
1104	38-39	0.5291049363940772
1104	40-41	0.5342308967894809
1104	42-43	0.5414686951665146
1104	44-45	0.5718683832393552
1104	46-47	0.5339972768894441
1104	48-49	0.5802555753270653
1104	50-51	0.5397371829146564
1104	52-53	0.5373887763716283
1104	54-55	0.5291750877713355
1104	56-57	0.5297825994122505
1104	58-59	0.5721850928230054
1104	60-61	0.5584468833491911
1104	62-63	0.5784115400227066
1104	64-65	0.5441927980239143
1104	66-67	0.4359393055349159
1104	68-69	0.4832571753619419
1104	70-71	0.4488275204933885
1104	72-73	0.48917736523727484
1104	74-75	0.4374776363103621
1104	76-77	0.4188435636957095
1104	78-79	0.4180886257702454
1104	80-81	0.37484349681790974
1104	82-83	0.33267648570668484
1104	84-85	0.314791000753857
1104	86-87	0.30284036574242634
1104	88-89	0.29717563936528535
1104	90-91	0.34198294192788836
1104	92-93	0.40617319423559195
1104	94-95	0.3517023830531443
1104	96-97	0.3527653562649391
1104	98-99	0.2978111756288726
1104	100	0.5130689303391023
1105	1	0.19524911128114297
1105	2	-0.35827995885193786
1105	3	0.0797049608381073
1105	4	0.16297288896734585
1105	5	0.19537651129490285
1105	6	0.2230256327536111
1105	7	0.28488535045308083
1105	8	0.24971665768291018
1105	9	0.43028728364036084
1105	10-11	0.3955840147100105
1105	12-13	0.34868125966355734
1105	14-15	0.42262894095627246
1105	16-17	0.3893781202529638
1105	18-19	0.4986714546737758
1105	20-21	0.4694022765513495
1105	22-23	0.45795561130562845
1105	24-25	0.49194147781756925
1105	26-27	0.4030704895909025
1105	28-29	0.4326845422034111
1105	30-31	0.5350272262113762
1105	32-33	0.5080237756432453
1105	34-35	0.5226451685625548
1105	36-37	0.4687517463362454
1105	38-39	0.43666214371553025
1105	40-41	0.5353370498642676
1105	42-43	0.5333510953303104
1105	44-45	0.5752679749499876
1105	46-47	0.5636798213079928
1105	48-49	0.5019095656508412
1105	50-51	0.5484543037178398
1105	52-53	0.5687766975443651
1105	54-55	0.5276132468714394
1105	56-57	0.5266691875474265
1105	58-59	0.44493367984210863
1105	60-61	0.4826645197031212
1105	62-63	0.5009386369758602
1105	64-65	0.5094852014503104
1105	66-67	0.44121505220783774
1105	68-69	0.48429203346711347
1105	70-71	0.45543898031383634
1105	72-73	0.39135274826801236
1105	74-75	0.3746902267749803
1105	76-77	0.41314161472150346
1105	78-79	0.35528321682252084
1105	80-81	0.3796377456975648
1105	82-83	0.37030541933501837
1105	84-85	0.3662748874421027
1105	86-87	0.41721892873420785
1105	88-89	0.3689423773593603
1105	90-91	0.30615603054704366
1105	92-93	0.3314967944812892
1105	94-95	0.38701241941735987
1105	96-97	0.3412037877885794
1105	98-99	0.3894804800472542
1105	100	0.39579573212352415
1106	1	0.1901008608137147
1106	2	0.2926250090360938
1106	3	0.19797779778080837
1106	4	0.18255426427963073
1106	5	0.14528103526598812
1106	6	0.15205949497501337
1106	7	0.15790127893840378
1106	8	0.163309368973394
1106	9	0.20777984760803037
1106	10-11	0.20136341721813977
1106	12-13	0.22956765970896242
1106	14-15	0.2854811094142775
1106	16-17	0.2327676230575193
1106	18-19	0.3056293761767179
1106	20-21	0.2940150564456374
1106	22-23	0.23195693585550003
1106	24-25	0.23834127119287274
1106	26-27	0.27013033832027844
1106	28-29	0.23954006206488287
1106	30-31	0.25514045468204216
1106	32-33	0.2869556280391663
1106	34-35	0.2128787734985096
1106	36-37	0.26578089151656314
1106	38-39	0.21802833833921653
1106	40-41	0.2560989456098639
1106	42-43	0.2524124037331319
1106	44-45	0.22703827377216612
1106	46-47	0.19759294701696462
1106	48-49	0.20258707905518492
1106	50-51	0.2286535008550885
1106	52-53	0.23490837652464336
1106	54-55	0.177886861515951
1106	56-57	0.24252226417138445
1106	58-59	0.1496003037267002
1106	60-61	0.18597667802897178
1106	62-63	0.1131977866207663
1106	64-65	0.16625036007012994
1106	66-67	0.14920544064363384
1106	68-69	0.1666140791109214
1106	70-71	0.15201343890883834
1106	72-73	0.11100437513040617
1106	74-75	0.16081312060512687
1106	76-77	0.15733696655532725
1106	78-79	0.17617686864084448
1106	80-81	0.22426766383556185
1106	82-83	0.24550032132171395
1106	84-85	0.15863136206385775
1106	86-87	0.15335965548722896
1106	88-89	0.1050074412656059
1106	90-91	0.1631944967123644
1106	92-93	0.161102051508351
1106	94-95	0.1356760621116706
1106	96-97	0.16014217186314283
1106	98-99	0.15307167702184188
1106	100	0.22950700905398946
1107	1	0.09697384335543546
1107	2	-0.4315608937498894
1107	3	-0.041110142757126766
1107	4	0.049067759733723904
1107	5	0.09618685100829794
1107	6	0.11451111091066224
1107	7	0.15254155683791737
1107	8	0.1657084055477469
1107	9	0.1855597002740268
1107	10-11	0.1723517955151408
1107	12-13	0.1878415252638277
1107	14-15	0.24186121602119215
1107	16-17	0.2832249612384743
1107	18-19	0.24359114466746945
1107	20-21	0.2037143860873698
1107	22-23	0.24859862446729153
1107	24-25	0.28292027107981
1107	26-27	0.31394237059707564
1107	28-29	0.19804147722334164
1107	30-31	0.2533786522732271
1107	32-33	0.3141663843867377
1107	34-35	0.2845753979517909
1107	36-37	0.35870719529798833
1107	38-39	0.4337495705433483
1107	40-41	0.3394852362305656
1107	42-43	0.3190942033217965
1107	44-45	0.3529749111320939
1107	46-47	0.3659494037091804
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2216	82-83	-0.6527736408964699
2216	84-85	-0.6269632274355104
2216	86-87	-0.598998953454533
2216	88-89	-0.5302561074802554
2216	90-91	-0.5957499234525514
2216	92-93	-0.5897363575740755
2216	94-95	-0.5800462198912584
2216	96-97	-0.5909186933615658
2216	98-99	-0.5965781697416404
2216	100	-0.6411743961067806
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	12.0
13	51.0
14	219.0
15	734.0
16	1588.0
17	3240.0
18	5760.0
19	9123.0
20	12900.0
21	18747.0
22	25510.0
23	31059.0
24	33120.0
25	35022.0
26	39221.0
27	47710.0
28	59446.0
29	75012.0
30	92029.0
31	113694.0
32	141548.0
33	178009.0
34	238807.0
35	329638.0
36	495545.0
37	841339.0
38	792474.0
39	265981.0
40	714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.01548543785466	20.30973447913004	13.676947164621293	24.997832918394007
2	15.18843051770136	19.971034628561934	39.36240175448568	25.47813309925102
3	18.320725931229735	25.28151901963689	29.63817116027192	26.759583888861453
4	12.579981436280342	16.72394081836779	37.47280785521122	33.22326989014065
5	13.079006529331325	38.01641244531135	34.78639053513152	14.118190490225807
6	31.094925615732507	37.78234709400489	17.08571068512739	14.037016605135207
7	27.128329078621217	31.950821940426486	22.723814745908044	18.197034235044253
8	26.487666060996514	34.925795761852044	19.38018468438927	19.206353492762176
9	26.008453663661463	14.712872483929166	20.730816265908487	38.54785758650088
10-11	25.6790899773806	25.69220640107195	27.246541186213353	21.382162435334102
12-13	26.07682623696234	23.40200166912921	27.753953894484802	22.76721819942365
14-15	23.298899732311707	24.553118732586277	25.39548485789629	26.752496677205727
16-17	22.258356346708418	27.2624232039119	26.567951879273817	23.911268570105868
18-19	21.85556245668044	26.765168436740904	29.043761275945123	22.335507830633535
20-21	23.038012968295266	25.94911543799116	28.669142329252324	22.343729264461253
22-23	23.203049411222494	25.649010628497415	28.435384157196626	22.71255580308346
24-25	22.860846317949825	25.803734580165138	28.914680235735567	22.420738866149467
26-27	22.743136372404038	25.988851025525623	28.273053430206392	22.99495917186395
28-29	22.17228551110974	26.30753384230201	28.740579000091298	22.77960164649695
30-31	22.8698606444279	25.970030257789162	28.506978065996186	22.653131031786753
32-33	23.07303661925465	26.148567930858448	27.984224285701426	22.794171164185475
34-35	23.097640395891506	26.042959829866337	28.48441337295026	22.374986401291896
36-37	22.955837515800788	25.72173121361639	28.2078593096389	23.11457196094392
38-39	22.97484347091433	25.826559729235864	28.63265140789377	22.56594539195603
40-41	22.723896575181286	25.337653202545628	29.006271483076514	22.932178739196576
42-43	22.830561866990962	25.458380811087427	28.337599847951168	23.373457473970447
44-45	22.959566695085584	26.08783374547194	28.10665709939292	22.84594246004956
46-47	23.08072644412468	26.052625149327913	27.473699641613013	23.3929487649344
48-49	23.628846783210626	26.017875731453817	27.272854696832045	23.080422788503515
50-51	23.277344207105756	26.058977613350976	27.15113849271717	23.512539686826095
52-53	22.925361119576777	26.38704251650162	27.050977880823147	23.63661848309846
54-55	22.876594939196078	26.3626616285637	26.99146862484685	23.76927480739338
56-57	23.546528187934392	26.044682563533396	26.783948204983194	23.62484104354902
58-59	22.79998374587603	26.229976822442215	27.36624810582875	23.603791325853006
60-61	22.819127581592642	26.68822135619401	26.974288426487092	23.518362635726263
62-63	23.09894798566452	26.179725866744853	26.740684445850388	23.98064170174024
64-65	22.582662402543043	26.227587953979352	27.028165590991332	24.16158405248627
66-67	22.908489798122808	26.059633434535545	26.994152904066222	24.037723863275428
68-69	22.735564717752357	25.89207754026993	27.266171304503708	24.10618643747401
70-71	22.620853438924012	25.73034667259225	27.31245798208024	24.33634190640349
72-73	23.03602772966279	25.806293568708842	27.39442243371384	23.763256267914524
74-75	23.129242515484815	26.102409454960974	27.04678165171969	23.72156637783452
76-77	22.96337485095732	26.104883353205693	26.73325449545645	24.198487300380542
78-79	22.78500985280207	26.090392193473953	26.846545243933363	24.278052709790614
80-81	22.510146906058466	26.520904259655858	27.140456430094563	23.828492404191117
82-83	23.04630757455424	25.807628049759064	27.78585640156207	23.360207974124627
84-85	23.253683077896877	26.132784604805764	26.7963359742244	23.817196343072954
86-87	22.299231121306185	26.592860553592146	27.11722638331484	23.99068194178683
88-89	22.906530642899543	25.81481285243527	26.962603409304858	24.316053095360328
90-91	22.61456606746425	25.87360769767822	27.08346220109698	24.42836403376055
92-93	22.84194408871195	26.04347221089794	26.90194137774315	24.212642322646953
94-95	22.08872401362896	25.94353622454858	27.346257592217672	24.621482169604793
96-97	22.372832662820024	25.888012189093494	27.007694828225652	24.731460319860826
98-99	21.902823918343266	26.402526735523235	26.88293157285197	24.81171777328153
100	22.40637807489136	25.920672011416414	26.45975452864836	25.213195385043864
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	270.0
1	211.5
2	221.5
3	292.0
4	207.5
5	177.5
6	375.5
7	927.5
8	1559.0
9	1809.0
10	1446.0
11	973.5
12	910.0
13	1031.5
14	1187.0
15	1461.5
16	1810.5
17	2181.0
18	2796.0
19	3504.5
20	4228.5
21	5138.5
22	6307.5
23	7429.0
24	8587.0
25	10127.5
26	12304.0
27	14885.0
28	18749.0
29	25135.0
30	33400.0
31	41180.0
32	51762.5
33	62106.0
34	69302.5
35	77479.0
36	86075.0
37	100921.5
38	108824.0
39	105550.5
40	101893.5
41	98278.5
42	99752.0
43	106641.5
44	115751.5
45	133626.0
46	151019.5
47	162434.0
48	170694.5
49	169639.5
50	164716.0
51	168083.0
52	170362.5
53	165956.0
54	161945.0
55	146151.5
56	130742.0
57	113298.0
58	95130.5
59	89228.0
60	75753.5
61	57342.5
62	44578.5
63	35499.5
64	26017.5
65	16099.5
66	11068.0
67	8372.0
68	6295.5
69	4777.0
70	3611.5
71	2857.5
72	2291.0
73	1775.5
74	1345.5
75	977.0
76	667.5
77	419.5
78	223.0
79	112.5
80	58.0
81	26.0
82	18.0
83	8.0
84	3.0
85	1.5
86	0.5
87	1.5
88	1.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013836541070480202
2	0.004217830363492106
3	0.001620264103048797
4	0.02836748104226703
5	0.002777595605226509
6	0.0
7	4.372141230449135E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.00867998626633284
16-17	0.003021921144575137
18-19	0.0
20-21	7.715543347851414E-5
22-23	7.458358569589701E-4
24-25	0.0
26-27	1.285923891308569E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.629326008710848E-4
36-37	0.0
38-39	0.0
40-41	5.143695565234276E-4
42-43	7.84413573698227E-4
44-45	0.0
46-47	0.0
48-49	5.786657510888561E-4
50-51	0.0
52-53	0.0
54-55	4.886510786972562E-4
56-57	1.157331502177712E-4
58-59	2.8290325608788515E-4
60-61	1.5431086695702828E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0014402347582655973
70-71	8.487097682636555E-4
72-73	0.0
74-75	2.700440171747995E-4
76-77	0.0027518771274003375
78-79	5.40088034349599E-4
80-81	0.0031890912504452516
82-83	0.0020317597482675392
84-85	0.006082420005889531
86-87	0.008242772143287927
88-89	0.007895572692634613
90-91	0.005799516749801646
92-93	0.008898593327855298
94-95	0.001620264103048797
96-97	0.004860792309146391
98-99	0.0019546043147890247
100	0.08541106486071516
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3888255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.69719448397783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17002840175923	25.048731508038475
2	15.447252091026456	11.028471242728093
3	5.729163374501703	6.135451776302101
4	2.751666033196591	3.9290703016789803
5	1.486626931700971	2.65342053530244
6	0.9408739612700121	2.015193646821975
7	0.6157991449014428	1.5387611287029836
8	0.4320987538987295	1.2339770603365938
9	0.3140079067570674	1.008828118531243
>10	1.7298518647612373	11.519238839730129
>50	0.1689760709026121	4.21521526937003
>100	0.17112906686813156	13.084266207586051
>500	0.029336007071628725	7.161265491120099
>1k	0.012541683611372551	7.708775284352322
>5k	5.766291315573586E-4	1.434913585549363
>10k+	7.207864144466982E-5	0.28442000384920846
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	11054	0.28429205389049844	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8612	0.22148753103898794	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7993	0.20556779326458785	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7895	0.20304738243762305	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7570	0.19468887714411734	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	6344	0.16315802332923124	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6146	0.1580657647196493	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	6071	0.15613687888268646	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	5137	0.1321158205930424	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4968	0.1277693978404194	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4407	0.11334133177993727	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	3984	0.10246241565946679	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	2.571847782617138E-5
10-11	2.571847782617138E-5	0.0	0.0	0.0	2.571847782617138E-5
12-13	7.715543347851414E-5	0.0	0.0	0.0	7.715543347851414E-5
14-15	7.715543347851414E-5	0.0	0.0	0.0	1.0287391130468552E-4
16-17	9.001467239159983E-5	0.0	0.0	0.0	1.0287391130468552E-4
18-19	1.0287391130468552E-4	0.0	0.0	0.0	1.0287391130468552E-4
20-21	1.0287391130468552E-4	0.0	0.0	0.0	1.0287391130468552E-4
22-23	1.0287391130468552E-4	0.0	0.0	3.857771673925707E-5	1.0287391130468552E-4
24-25	2.1860706152245673E-4	0.0	0.0	6.429619456542845E-5	1.0287391130468552E-4
26-27	3.0862173391405656E-4	0.0	0.0	2.571847782617138E-4	1.1573315021777122E-4
28-29	3.0862173391405656E-4	0.0	0.0	0.0012987831302216546	1.285923891308569E-4
30-31	3.343402117402279E-4	0.0	0.0	0.005426598821322162	1.414516280439426E-4
32-33	3.6005868956639933E-4	0.0	0.0	0.01593259701331317	1.5431086695702828E-4
34-35	3.8577716739257074E-4	0.0	0.0	0.032083801088148796	1.5431086695702828E-4
36-37	4.114956452187421E-4	0.0	0.0	0.06245732340085719	1.5431086695702828E-4
38-39	4.114956452187421E-4	0.0	0.0	0.11119383888145197	1.5431086695702828E-4
40-41	4.114956452187421E-4	0.0	0.0	0.16594847817337083	1.5431086695702828E-4
42-43	4.114956452187421E-4	0.0	0.0	0.22422654892747518	1.5431086695702828E-4
44-45	4.3721412304491346E-4	0.0	0.0	0.2942193863314006	1.6717010587011396E-4
46-47	4.6293260087108487E-4	0.0	0.0	0.38519850164148184	1.8002934478319966E-4
48-49	4.6293260087108487E-4	0.0	0.0	0.49280461286618293	1.8002934478319966E-4
50-51	4.6293260087108487E-4	0.0	0.0	0.6066731734415567	2.3146630043554243E-4
52-53	5.143695565234276E-4	0.0	0.0	0.7208760742286707	2.9576249500097085E-4
54-55	5.40088034349599E-4	0.0	0.0	0.8487869236971342	3.2148097282714227E-4
56-57	5.40088034349599E-4	0.0	0.0	1.0073670579733067	3.343402117402279E-4
58-59	5.40088034349599E-4	0.0	0.0	1.1971437058526253	3.343402117402279E-4
60-61	5.658065121757704E-4	0.0	0.0	1.403444475735259	3.343402117402279E-4
62-63	5.658065121757704E-4	0.0	0.0	1.6202383845709707	3.6005868956639933E-4
64-65	5.658065121757704E-4	0.0	0.0	1.84269035852844	3.6005868956639933E-4
66-67	5.658065121757704E-4	0.0	0.0	2.1044916035599517	3.72917928479485E-4
68-69	6.429619456542845E-4	0.0	0.0	2.406066474549637	3.857771673925707E-4
70-71	6.686804234804558E-4	0.0	0.0	2.7390178884872523	3.857771673925707E-4
72-73	6.686804234804558E-4	0.0	0.0	3.0845199196040385	4.757918397841705E-4
74-75	6.686804234804558E-4	0.0	0.0	3.4311792822230025	4.886510786972562E-4
76-77	6.686804234804558E-4	0.0	0.0	3.8159277105025264	5.143695565234276E-4
78-79	6.943989013066272E-4	0.0	0.0	4.250274223269821	5.143695565234276E-4
80-81	6.943989013066272E-4	0.0	0.0	4.722992704953764	5.143695565234276E-4
82-83	6.943989013066272E-4	0.0	0.0	5.219603652538221	5.40088034349599E-4
84-85	6.943989013066272E-4	0.0	0.0	5.699664759641536	5.40088034349599E-4
86-87	6.943989013066272E-4	0.0	0.0	6.213237043352352	5.40088034349599E-4
88	6.943989013066272E-4	0.0	0.0	6.628294697750019	5.40088034349599E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7410	0.0	44.230064	1
GTATCAA	12335	0.0	38.578415	1
CAACGCA	14640	0.0	31.204006	5
GTACTAG	3110	0.0	30.84415	1
ATCAACG	14860	0.0	30.719492	3
TATCAAC	16090	0.0	29.57024	2
GGGCACC	3730	0.0	29.510956	94
GTGGTAT	2585	0.0	29.286558	1
TAGTACT	4310	0.0	28.46784	4
AACGCAG	16505	0.0	28.303442	6
CTGTGCG	4435	0.0	27.662987	9
TCAACGC	16795	0.0	27.346758	4
TAGGCAT	3955	0.0	26.499855	5
CTAGTAC	4750	0.0	26.12907	3
ACGCAGA	17540	0.0	25.990253	7
CTAACGC	1390	0.0	25.704693	3
CGCAGAG	17815	0.0	25.53564	8
GTACATG	14390	0.0	25.422709	1
CTAGGCA	4325	0.0	25.216984	4
TCTAACG	1455	0.0	24.880444	2
>>END_MODULE