FastQCFastQC Report
Mon 16 Jan 2017
ERR1146428_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146428_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4785199
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC180850.3779362154008642No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT157470.32907722332968803No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA150980.31551456898657715No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN144420.3018056302360675No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC131380.2745549349149325No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA124430.26003098303748706No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC113690.2375867753880246No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC99080.20705512978666088No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT84190.17593834655570229No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC76990.16089195036611853No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG76280.15940820851964568No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC74740.1561899515568736No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT72980.1525119435994198No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT70340.14699493166323907No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT68880.14394385688035127No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG68770.1437139813830104No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC66600.13917916475364975No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG62840.13132160229908935No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA61130.1277480832040632No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA61110.12770628765909212No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT59190.1236939153418698No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA59080.12346403984452893No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC58340.12191760468059948No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC57080.11928448534742234No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG57040.1192008942574802No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT56400.1178634368184061No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT55160.11527211303019999No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA54670.11424812217840888No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA54040.1129315625118203No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT53590.11199116274997131No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA53190.11115525185055No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG51520.10766532384546598No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC51520.10766532384546598No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT49850.10417539584038198No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG49730.10392462257055558No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC47900.10010033020570305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC50750.036.75120594
TATTCTC23150.033.4850545
GTACTAG53900.033.0854841
CAACGCA99450.032.2179265
GTATCAA113250.032.1181561
ATCAACG106000.030.4098223
CGTTAAC11650.029.966791
TAGGCAT65750.029.6531165
AGGGCTA56050.029.3366455
GCATCGT58600.027.8812398
GCTATTG59350.027.6080138
TATCAAC132800.027.3869112
CTATTGA60400.027.2866889
CTGTGCG74400.027.1378969
CTAACGC27500.026.997283
TAGTACT76300.026.9711534
GGTATCA75900.026.727671
CTAGGCA77700.026.5456584
TCTAACG28100.026.4211032
AACGCAG129600.026.043246
Produced by FastQC (version 0.11.5)