##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146428_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4785199
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.567500118594857	31.0	31.0	34.0	27.0	34.0
2	30.701102921738467	31.0	31.0	34.0	27.0	34.0
3	30.874905306968426	31.0	31.0	34.0	27.0	34.0
4	34.34655967285791	37.0	35.0	37.0	30.0	37.0
5	34.564144354289134	37.0	35.0	37.0	30.0	37.0
6	34.684063296009214	37.0	35.0	37.0	31.0	37.0
7	34.581280736705	37.0	35.0	37.0	30.0	37.0
8	34.53093695789872	37.0	35.0	37.0	30.0	37.0
9	35.915820846740125	39.0	35.0	39.0	30.0	39.0
10-11	35.8409159159316	39.0	35.0	39.0	30.0	39.0
12-13	35.82740592397516	39.0	35.0	39.0	30.0	39.0
14-15	36.85373837117328	40.0	36.0	41.0	30.0	41.0
16-17	36.68778393542254	40.0	36.0	41.0	29.0	41.0
18-19	36.63099800029215	40.0	36.0	41.0	28.5	41.0
20-21	36.965592026580296	40.0	36.0	41.0	30.0	41.0
22-23	36.67278111108858	40.0	36.0	41.0	29.0	41.0
24-25	36.782407481904094	40.0	36.0	41.0	30.0	41.0
26-27	36.852569663247024	40.0	36.0	41.0	30.0	41.0
28-29	36.68853029936686	40.0	35.5	41.0	29.5	41.0
30-31	36.63262238414745	40.0	35.0	41.0	29.5	41.0
32-33	36.73554328670553	40.0	35.5	41.0	30.0	41.0
34-35	36.781759233001594	40.0	35.0	41.0	30.0	41.0
36-37	36.71718062300022	40.0	35.0	41.0	30.0	41.0
38-39	36.543400493897956	39.0	35.0	41.0	29.0	41.0
40-41	36.49850894393316	39.0	35.0	41.0	29.0	41.0
42-43	36.333717155754655	39.0	35.0	41.0	29.0	41.0
44-45	36.14059310804002	39.0	35.0	41.0	28.0	41.0
46-47	35.999430013255456	39.0	35.0	41.0	28.0	41.0
48-49	35.863696368740364	38.0	34.0	40.0	28.0	41.0
50-51	35.6313468886038	38.0	34.0	40.0	27.5	41.0
52-53	35.43252130162194	38.0	34.0	40.0	27.0	41.0
54-55	35.2299246071062	37.5	34.0	40.0	27.0	41.0
56-57	35.07377540620568	37.0	33.0	40.0	27.0	41.0
58-59	34.796734472275865	36.5	33.0	40.0	26.5	41.0
60-61	34.538905592014046	36.0	33.0	39.5	26.0	41.0
62-63	34.261280042898946	35.5	33.0	39.0	26.0	41.0
64-65	33.968546553654306	35.0	33.0	39.0	26.0	41.0
66-67	33.681823368265356	35.0	32.0	38.5	26.0	40.5
68-69	33.39349377110544	35.0	32.0	38.0	25.0	40.0
70-71	33.34223069928753	35.0	32.0	37.0	26.0	40.0
72-73	33.14446013634961	35.0	32.0	37.0	25.5	40.0
74-75	32.883941608280026	35.0	32.0	36.0	25.0	39.0
76-77	32.670171292771734	35.0	32.0	36.0	25.0	39.0
78-79	32.4640218724446	35.0	32.0	36.0	25.0	39.0
80-81	32.25694971097336	35.0	31.5	35.0	24.5	37.5
82-83	32.03206982614516	35.0	31.0	35.0	24.0	37.0
84-85	31.86689184713112	35.0	31.0	35.0	24.0	37.0
86-87	31.710427194354928	35.0	31.0	35.0	24.0	36.0
88-89	31.722233181942904	35.0	32.0	35.0	24.0	36.0
90-91	31.6523604347489	35.0	32.0	35.0	24.0	36.0
92-93	31.510682627828018	35.0	31.0	35.0	24.0	36.0
94-95	31.50328272659089	35.0	31.0	35.0	24.0	35.0
96-97	31.417543345637245	35.0	31.0	35.0	23.5	35.0
98-99	31.34121308225635	35.0	31.0	35.0	23.0	35.0
100	30.845825638599354	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6835881774574695
1101	2	0.8883355000167619
1101	3	0.8913793910552847
1101	4	0.9200427252008723
1101	5	0.8632694797448366
1101	6	0.5497971842462093
1101	7	0.6354237368324931
1101	8	0.6157076937813741
1101	9	0.8818068635163314
1101	10-11	0.8696017231622903
1101	12-13	0.7029219898449526
1101	14-15	0.971741706052434
1101	16-17	1.0461951177067803
1101	18-19	1.1896247003351519
1101	20-21	0.8751596472930672
1101	22-23	1.1750705711191358
1101	24-25	1.0637612932258094
1101	26-27	0.9467713177561023
1101	28-29	1.1584050659972718
1101	30-31	1.1514577395383725
1101	32-33	1.0404231970563202
1101	34-35	0.8968726834865848
1101	36-37	0.8656420580233615
1101	38-39	0.9901593618038547
1101	40-41	0.8959665329113946
1101	42-43	0.932881213870445
1101	44-45	0.9588838566906759
1101	46-47	0.965193870266198
1101	48-49	0.9161254148053501
1101	50-51	0.9471486453046083
1101	52-53	0.9953314592504654
1101	54-55	0.9693394584926125
1101	56-57	0.828352157910011
1101	58-59	0.8070572529540883
1101	60-61	0.8440600920236321
1101	62-63	0.7656708120912228
1101	64-65	0.7415689138682211
1101	66-67	0.8161498959484703
1101	68-69	0.8264727273396844
1101	70-71	0.7440017441138522
1101	72-73	0.7231769741801486
1101	74-75	0.6639981409781299
1101	76-77	0.7200763830746126
1101	78-79	0.6873932754470857
1101	80-81	0.7435312271588472
1101	82-83	0.6989896732610745
1101	84-85	0.6565611785655463
1101	86-87	0.6322418328795791
1101	88-89	0.5707540195495717
1101	90-91	0.6010969879038086
1101	92-93	0.6670672607148731
1101	94-95	0.6263300460918977
1101	96-97	0.5879784560780408
1101	98-99	0.562525295969337
1101	100	0.6357609919244709
1102	1	0.70863370357619
1102	2	0.8124224560818156
1102	3	0.8080071065133723
1102	4	0.938568418252963
1102	5	0.7253377626610984
1102	6	0.5300685485969581
1102	7	0.5940553313865635
1102	8	0.6428561138435995
1102	9	0.8121033896071808
1102	10-11	0.8071332763344969
1102	12-13	0.7419388465275318
1102	14-15	0.9996257215402764
1102	16-17	1.176272156935525
1102	18-19	1.1850170787181682
1102	20-21	0.9537545677861416
1102	22-23	1.1969150956699792
1102	24-25	1.0137960160231572
1102	26-27	0.9406404581241361
1102	28-29	1.0810435218892138
1102	30-31	1.1550651533706926
1102	32-33	1.078836629830036
1102	34-35	0.9060259145112184
1102	36-37	0.8199061346938805
1102	38-39	0.8805351746452104
1102	40-41	0.8167335128440882
1102	42-43	0.8763930736886465
1102	44-45	0.9168279304309337
1102	46-47	0.9105726948405959
1102	48-49	0.8267561254329507
1102	50-51	0.9308611149566701
1102	52-53	0.8734499293681921
1102	54-55	0.9118321230744257
1102	56-57	0.8104308987376285
1102	58-59	0.7461735176994964
1102	60-61	0.7900917617464742
1102	62-63	0.7408311853602854
1102	64-65	0.7673085592768842
1102	66-67	0.7463803026152505
1102	68-69	0.7918678272574482
1102	70-71	0.748024592616737
1102	72-73	0.7098693964497684
1102	74-75	0.6930550019322084
1102	76-77	0.7016397694502672
1102	78-79	0.6970877161770801
1102	80-81	0.7763220849567034
1102	82-83	0.7588298393164408
1102	84-85	0.6786438542395388
1102	86-87	0.7125994218071767
1102	88-89	0.7776002213253115
1102	90-91	0.7203310475677611
1102	92-93	0.7459224170267049
1102	94-95	0.7473211856850845
1102	96-97	0.766823351086181
1102	98-99	0.7559034693145357
1102	100	0.7933801378473468
1103	1	0.5378579068129916
1103	2	0.7375185750062876
1103	3	0.6523809152688891
1103	4	0.6174952383706156
1103	5	0.6314107413658547
1103	6	0.4367498563739005
1103	7	0.5065875764246073
1103	8	0.5534645148600887
1103	9	0.5364001540052996
1103	10-11	0.7020333043927707
1103	12-13	0.5835022309262214
1103	14-15	0.7479526833615608
1103	16-17	0.9402860416535717
1103	18-19	0.9774418362991639
1103	20-21	0.6967286068167269
1103	22-23	0.8892552653999175
1103	24-25	0.8679132154285227
1103	26-27	0.7603440982128902
1103	28-29	0.993740972369551
1103	30-31	0.9977506024750227
1103	32-33	0.7463480203455859
1103	34-35	0.7503580222155719
1103	36-37	0.7275024008377216
1103	38-39	0.8225530605324849
1103	40-41	0.711102152268019
1103	42-43	0.7108682120015715
1103	44-45	0.8282575623718671
1103	46-47	0.7331650320259726
1103	48-49	0.7779701589424803
1103	50-51	0.8238604144322963
1103	52-53	0.875220158974443
1103	54-55	0.8431907273335781
1103	56-57	0.7288229408447648
1103	58-59	0.6402836239292569
1103	60-61	0.6603292152591251
1103	62-63	0.6553658802351592
1103	64-65	0.6577334516868376
1103	66-67	0.671683860953955
1103	68-69	0.6663686470228427
1103	70-71	0.622546729213397
1103	72-73	0.6170101485555293
1103	74-75	0.6337032814107886
1103	76-77	0.5942953350217763
1103	78-79	0.6026085223370075
1103	80-81	0.6191659447633597
1103	82-83	0.4812566513070564
1103	84-85	0.5329208632642803
1103	86-87	0.5929992749342077
1103	88-89	0.5437779642619169
1103	90-91	0.6090285438792833
1103	92-93	0.6379304172334841
1103	94-95	0.5794842471870716
1103	96-97	0.5692191897209611
1103	98-99	0.5670258857478956
1103	100	0.658781668342538
1104	1	0.4984781458054286
1104	2	0.6376108052328746
1104	3	0.6160279392132111
1104	4	0.6636684264762565
1104	5	0.6137034925155476
1104	6	0.31981370313276614
1104	7	0.27646361454502255
1104	8	0.34348137511830146
1104	9	0.46116198098054184
1104	10-11	0.5267689579253414
1104	12-13	0.40469001728941123
1104	14-15	0.5934492866480099
1104	16-17	0.6630118293628158
1104	18-19	0.6716109925808738
1104	20-21	0.5804691975038594
1104	22-23	0.7808063879409559
1104	24-25	0.6317010774215532
1104	26-27	0.5679563006059922
1104	28-29	0.7042031630151016
1104	30-31	0.8052440034261821
1104	32-33	0.611386797878751
1104	34-35	0.44028334171985506
1104	36-37	0.5490027039405021
1104	38-39	0.6675460835995963
1104	40-41	0.5748131645782948
1104	42-43	0.5244985825294748
1104	44-45	0.6041216704939032
1104	46-47	0.5127975059758327
1104	48-49	0.5077525666764586
1104	50-51	0.5736430979553333
1104	52-53	0.5619809315868878
1104	54-55	0.5136570070823367
1104	56-57	0.47352805604239023
1104	58-59	0.4789289904873968
1104	60-61	0.4922262356234981
1104	62-63	0.4407667210735795
1104	64-65	0.4637039136176071
1104	66-67	0.5455880635019241
1104	68-69	0.5343417936540504
1104	70-71	0.3876162147978519
1104	72-73	0.4135105709901268
1104	74-75	0.4195854271981929
1104	76-77	0.4920289058165608
1104	78-79	0.44063044650668104
1104	80-81	0.4831121733214232
1104	82-83	0.46760297129979733
1104	84-85	0.3941504369739519
1104	86-87	0.43928479404612375
1104	88-89	0.3308644331489283
1104	90-91	0.3639439535400726
1104	92-93	0.3753772416550305
1104	94-95	0.30805689559474203
1104	96-97	0.36806156513458177
1104	98-99	0.3714769886124394
1104	100	0.4901226024931269
1105	1	0.5141086084322133
1105	2	0.6937976715681664
1105	3	0.6471029997484763
1105	4	0.7371561813361254
1105	5	0.656470546097573
1105	6	0.33888057254527837
1105	7	0.409327735120975
1105	8	0.4323676608030951
1105	9	0.45323570595047613
1105	10-11	0.586249202634292
1105	12-13	0.5003557470813291
1105	14-15	0.6109243520788539
1105	16-17	0.742739887614718
1105	18-19	0.7415253747015456
1105	20-21	0.5561386506716204
1105	22-23	0.8434332197061707
1105	24-25	0.6994965257352348
1105	26-27	0.560748664477579
1105	28-29	0.8019445912117646
1105	30-31	0.8215640089542262
1105	32-33	0.6542299457687548
1105	34-35	0.5416181544704628
1105	36-37	0.4494504060558455
1105	38-39	0.5638569274033642
1105	40-41	0.4991388196335933
1105	42-43	0.581628246698159
1105	44-45	0.5510196833682102
1105	46-47	0.5319507701745891
1105	48-49	0.5663459630132408
1105	50-51	0.616372104383359
1105	52-53	0.6094631146102003
1105	54-55	0.49629296636359754
1105	56-57	0.4633740650327596
1105	58-59	0.4944810592529265
1105	60-61	0.5317187633676994
1105	62-63	0.464682265057796
1105	64-65	0.5058809892532992
1105	66-67	0.5364454008138537
1105	68-69	0.45943988955553294
1105	70-71	0.4324451314558857
1105	72-73	0.42492289274908757
1105	74-75	0.42088944493964675
1105	76-77	0.46948433268244116
1105	78-79	0.4996025469581582
1105	80-81	0.5664040260080156
1105	82-83	0.5277885897188455
1105	84-85	0.5293083334232023
1105	86-87	0.541889929431278
1105	88-89	0.4280401902473834
1105	90-91	0.5044984833977324
1105	92-93	0.5303061269251117
1105	94-95	0.4264459745335749
1105	96-97	0.47455665142636505
1105	98-99	0.5057165461130175
1105	100	0.6411096353692898
1106	1	0.5512824410544503
1106	2	0.5093132092319337
1106	3	0.5172533889630309
1106	4	0.7035223947417109
1106	5	0.5534659002874633
1106	6	0.3017863214997192
1106	7	0.29581339515527105
1106	8	0.34210927798955026
1106	9	0.578269465145695
1106	10-11	0.5245105524878539
1106	12-13	0.45144866988891863
1106	14-15	0.6306156423613558
1106	16-17	0.766834082272112
1106	18-19	0.651939069032089
1106	20-21	0.5230721323780969
1106	22-23	0.8424202572574444
1106	24-25	0.6519800363019286
1106	26-27	0.5533420267284725
1106	28-29	0.738209423099832
1106	30-31	0.7222392413725487
1106	32-33	0.6243688390373805
1106	34-35	0.5441180422352474
1106	36-37	0.48687790145513077
1106	38-39	0.6152919651582422
1106	40-41	0.5723287921832636
1106	42-43	0.5623645324600943
1106	44-45	0.5607830553401882
1106	46-47	0.49790120016327677
1106	48-49	0.49010042177398816
1106	50-51	0.5106999830169272
1106	52-53	0.49450899184977004
1106	54-55	0.6084681620419161
1106	56-57	0.5338976722497506
1106	58-59	0.5453183022216948
1106	60-61	0.45768865007226367
1106	62-63	0.5073199093714749
1106	64-65	0.49403129248615585
1106	66-67	0.5515132347317078
1106	68-69	0.5655938386508623
1106	70-71	0.5141373925403272
1106	72-73	0.46356046660554995
1106	74-75	0.5790450283588058
1106	76-77	0.5529530448823579
1106	78-79	0.52887863114006
1106	80-81	0.5375111462435171
1106	82-83	0.4929105969524272
1106	84-85	0.442518447855349
1106	86-87	0.44997160367689304
1106	88-89	0.4827001477797488
1106	90-91	0.44049000434926455
1106	92-93	0.49193281428311053
1106	94-95	0.5190788364889691
1106	96-97	0.5575983473584643
1106	98-99	0.529498655973061
1106	100	0.4296000532809785
1107	1	0.6053596445840128
1107	2	0.7371806756083146
1107	3	0.6467317808646129
1107	4	0.7161585313882597
1107	5	0.6520454901088328
1107	6	0.4824179556030259
1107	7	0.446219705346266
1107	8	0.5536918262630479
1107	9	0.6695659228126232
1107	10-11	0.656767846342234
1107	12-13	0.5159099958144893
1107	14-15	0.7505709990597254
1107	16-17	0.8496171352224167
1107	18-19	0.9610546471015837
1107	20-21	0.7081867539468689
1107	22-23	0.9514641179425709
1107	24-25	0.6680294231606538
1107	26-27	0.6028200907749621
1107	28-29	0.7424200047915974
1107	30-31	0.789403299785107
1107	32-33	0.732408606833097
1107	34-35	0.5983435873580518
1107	36-37	0.6228465314438694
1107	38-39	0.6461102062013779
1107	40-41	0.48788187464548116
1107	42-43	0.6159034155329834
1107	44-45	0.6032364940037525
1107	46-47	0.5126918196716872
1107	48-49	0.5075719717725633
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2214	100	-1.1720284102087213
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2215	4	-1.983731502357692
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2215	22-23	-3.179263766326855
2215	24-25	-5.4952339811525555
2215	26-27	-3.5462033768606176
2215	28-29	-1.7450052750799898
2215	30-31	-3.812701949392334
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2215	100	-3.1959218392881006
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2216	3	-0.8143953096260397
2216	4	-0.8055033755574499
2216	5	-0.970710914507201
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2216	12-13	-1.1454037082260768
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2216	64-65	-1.38447340826918
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2216	90-91	-1.3964007039911692
2216	92-93	-1.3749402507323722
2216	94-95	-1.258439400646985
2216	96-97	-1.282390846379549
2216	98-99	-1.199882923994064
2216	100	-1.1663994961443365
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	19424.0
1	3312.0
2	504.0
3	543.0
4	500.0
5	255.0
6	237.0
7	422.0
8	828.0
9	1297.0
10	1938.0
11	3282.0
12	5329.0
13	7631.0
14	9809.0
15	12297.0
16	15057.0
17	17913.0
18	20993.0
19	23819.0
20	28034.0
21	31277.0
22	36439.0
23	42017.0
24	48228.0
25	54057.0
26	61391.0
27	71315.0
28	84820.0
29	100537.0
30	118634.0
31	145159.0
32	180504.0
33	229340.0
34	317918.0
35	434566.0
36	643777.0
37	983920.0
38	766026.0
39	261640.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.022938208085996	21.589198467127588	12.606664060659737	23.78119926412668
2	15.318054118864023	19.855953948252235	41.10190695612456	23.724084976759183
3	18.420254149078087	27.258671870849582	28.82775905792194	25.49331492215039
4	12.180739879791158	16.898143288199734	40.44329634578224	30.477820486226875
5	13.115769427005317	38.575712184833826	35.03225512622178	13.276263261939079
6	29.263387030761734	38.78018577281689	18.45577947158505	13.500647724836327
7	26.561359292630165	31.809316783231473	24.14628357801441	17.483040346123957
8	26.019792638942913	35.11174998382749	20.096703243156504	18.771754134073095
9	26.070068417326787	15.083657354600053	22.038508640917218	36.80776558715594
10-11	25.850593961411573	25.91823305960455	27.314903228503763	20.91626975048011
12-13	26.020227765624142	23.66990182342334	27.42096303425308	22.888907376699436
14-15	23.37189570460317	24.74724201227406	24.953364337991037	26.927497945131734
16-17	22.26043693843503	27.319030774605913	26.33681534164979	24.08371694530927
18-19	21.648605844443626	28.107685715747333	28.021580757616487	22.222127682192554
20-21	23.466678711623768	27.18321325141362	26.696716033483582	22.65339200347903
22-23	24.041756125302395	26.750028272201504	26.470760873457195	22.737454729038905
24-25	23.411411953964794	26.883884158840686	27.188670713190668	22.51603317400386
26-27	23.23628374132893	27.12253121416537	26.172220046567755	23.46896499793794
28-29	22.28884104395316	27.74752927584939	26.861145827863258	23.102483852334192
30-31	23.200817211691522	27.188725822788744	26.67543731034341	22.935019655176326
32-33	23.67968088791438	27.495352864083067	25.617907853003253	23.207058394999304
34-35	23.59615028473905	27.22955777603427	26.729314545342437	22.44497739388424
36-37	23.317237910756084	26.846792933031182	26.33387150509239	23.502097651120344
38-39	23.500958177410208	26.80641286573519	27.019993667477877	22.67263528937672
40-41	23.012786638272928	25.948281930087948	27.69009500416556	23.34883642747357
42-43	22.99125602597979	26.277284997747614	26.958453936973463	23.773005039299136
44-45	23.220157023121015	27.247491393525042	26.701495319208128	22.83085626414581
46-47	23.1503153927506	26.91896405684655	26.27101103659245	23.659709513810398
48-49	23.93852180546638	26.347586088389008	26.650021547764986	23.063870558379634
50-51	23.497080791844603	26.330042122276986	26.46880391411413	23.704073171764282
52-53	22.836987618860743	26.986656672231113	26.402169167866603	23.77418654104154
54-55	22.84694285540799	26.836313218782635	26.32831063112837	23.988433294681
56-57	24.001397018468477	26.233139273812995	26.091746299345342	23.673717408373182
58-59	22.851540608596096	26.658015785669363	27.08249581103165	23.407947794702892
60-61	22.868601950942004	27.39325888488669	26.300249865445757	23.437889298725548
62-63	23.4090249057991	26.583953680727873	25.77324037969199	24.233781033781035
64-65	22.49445467116484	26.76533479331964	26.371856999215527	24.368353536300003
66-67	23.32670866955603	26.38157853272801	26.255548856195077	24.036163941520886
68-69	22.571157505224328	26.499158959315906	26.629008076301986	24.300675459157784
70-71	22.599773224288825	26.118326330542512	26.777759592955036	24.504140852213627
72-73	23.32298332067639	26.34408257627177	27.083087556863	23.249846546188834
74-75	23.483757569289118	26.71264161099432	26.620450810493278	23.183150009223283
76-77	23.071912052102896	26.713320853656924	26.178803190489287	24.035963903750897
78-79	22.975105433407244	26.65820096391755	26.30951081357638	24.057182789098828
80-81	22.57447077371328	27.018037774274305	27.042344300366477	23.36514715164594
82-83	23.50268514902404	25.931041784880936	28.068121252813246	22.498151813281776
84-85	23.819204433958102	26.56248489903627	26.47507264220118	23.14323802480445
86-87	22.382674751698243	27.256170070174125	27.025284076390165	23.335871101737467
88-89	23.42388964950625	25.848478118415553	26.871592410149304	23.8560398219289
90-91	23.083994111236773	25.953905161753465	27.0777461482428	23.88435457876696
92-93	23.32590212483543	26.279083820946962	26.973416895867786	23.42159715834982
94-95	22.1223147376306	26.27079087467737	27.679303630499025	23.927590757193013
96-97	22.555091871375215	26.19825361667674	27.120094437473103	24.12656007447494
98-99	21.99039183546874	27.024811443257192	26.920270150925678	24.064526570348388
100	22.81304026050605	26.212185329780873	26.13657130651694	24.838203103196136
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16102.0
1	9500.5
2	2647.0
3	1856.0
4	967.5
5	514.0
6	382.5
7	341.5
8	326.5
9	315.5
10	324.0
11	325.0
12	310.5
13	331.0
14	340.0
15	411.0
16	500.5
17	538.0
18	667.0
19	860.5
20	1056.5
21	1363.0
22	1882.0
23	2592.0
24	3427.0
25	4543.0
26	6488.0
27	8818.5
28	12958.0
29	22328.5
30	35168.5
31	47770.5
32	64531.5
33	80338.5
34	92629.0
35	106689.0
36	119664.0
37	143739.0
38	154143.5
39	141691.0
40	131267.0
41	121588.0
42	119110.5
43	127641.0
44	141364.5
45	170148.5
46	199447.5
47	219425.5
48	230429.0
49	224197.0
50	214626.0
51	221511.5
52	224887.5
53	215759.0
54	205210.5
55	178224.5
56	155814.5
57	129391.0
58	102541.5
59	96204.0
60	79231.0
61	55211.5
62	41470.5
63	34582.0
64	25210.0
65	12403.5
66	6824.5
67	4725.5
68	3496.0
69	2608.5
70	2019.5
71	1670.5
72	1286.5
73	1036.0
74	882.5
75	684.0
76	487.0
77	322.5
78	218.5
79	144.0
80	106.5
81	86.5
82	66.5
83	51.5
84	39.5
85	34.0
86	29.0
87	26.5
88	21.0
89	12.5
90	9.5
91	14.0
92	13.5
93	7.5
94	8.0
95	8.0
96	4.0
97	5.5
98	6.5
99	4.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9911186556713734
2	0.4423431502012769
3	0.4780574433790528
4	0.46299014941698347
5	0.4290312691279924
6	0.4836998419501467
7	0.49943586463175305
8	0.5022779616897856
9	0.5163630603450348
10-11	0.5303436701378563
12-13	0.5288390305188979
14-15	0.5258193023947384
16-17	0.530761625587567
18-19	0.5289330704950829
20-21	0.5277523463496503
22-23	0.4901468047619336
24-25	0.5255058358074555
26-27	0.5052976898139451
28-29	0.5109296394987962
30-31	0.5145240563663078
32-33	0.5170108912920862
34-35	0.529016661585025
36-37	0.5472186214199242
38-39	0.535860682074037
40-41	0.5419628316398126
42-43	0.53930881453415
44-45	0.5492352564647781
46-47	0.5477724123907909
48-49	0.5191947085168245
50-51	0.5350561178333441
52-53	0.5269791287676856
54-55	0.5365712063385452
56-57	0.5237295251461852
58-59	0.5302914257066426
60-61	0.5232175297202896
62-63	0.5174601934005253
64-65	0.5294868614659495
66-67	0.5268119465878013
68-69	0.547814207935762
70-71	0.5314303543071041
72-73	0.5354740732830546
74-75	0.532997687243519
76-77	0.5208978769743954
78-79	0.5514817670069729
80-81	0.5257984046222529
82-83	0.5251192270164731
84-85	0.5343351446825931
86-87	0.8404352671644377
88-89	1.1061608932042324
90-91	1.0962240023873615
92-93	1.0962135535011188
94-95	1.1003513124532542
96-97	1.0936222297129128
98-99	1.0896307551681759
100	1.0856392806234392
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4785199.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.49515676259092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29244059442193	18.6860226246767
2	12.447708601983118	6.347125642852222
3	5.124062610339862	3.9191633853583685
4	2.708277212340605	2.7619180834070587
5	1.534112082771748	1.9556214020825289
6	0.9783641408082386	1.4966128284482174
7	0.673002340942543	1.2010810128744558
8	0.4631922587229641	0.9447327397888432
9	0.344921285642578	0.7914440023390735
>10	1.86244254620827	8.768649484226904
>50	0.2095364201285186	3.7976375668719164
>100	0.2636048022849852	15.077591706946244
>500	0.05261502611232202	9.348897509549332
>1k	0.04301134739895309	19.32020939738712
>5k	0.002134150825138894	3.483842163821195
>10k+	5.745790683066253E-4	2.0994504493699955
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	18085	0.3779362154008642	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	15747	0.32907722332968803	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	15098	0.31551456898657715	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	14442	0.3018056302360675	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	13138	0.2745549349149325	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	12443	0.26003098303748706	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	11369	0.2375867753880246	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9908	0.20705512978666088	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8419	0.17593834655570229	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7699	0.16089195036611853	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7628	0.15940820851964568	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7474	0.1561899515568736	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	7298	0.1525119435994198	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7034	0.14699493166323907	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6888	0.14394385688035127	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6877	0.1437139813830104	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6660	0.13917916475364975	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6284	0.13132160229908935	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	6113	0.1277480832040632	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6111	0.12770628765909212	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	5919	0.1236939153418698	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5908	0.12346403984452893	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5834	0.12191760468059948	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5708	0.11928448534742234	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5704	0.1192008942574802	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5640	0.1178634368184061	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5516	0.11527211303019999	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5467	0.11424812217840888	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5404	0.1129315625118203	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5359	0.11199116274997131	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5319	0.11115525185055	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5152	0.10766532384546598	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	5152	0.10766532384546598	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4985	0.10417539584038198	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	4973	0.10392462257055558	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4790	0.10010033020570305	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	4.179554497106599E-5	0.0
12-13	0.0	0.0	0.0	4.179554497106599E-5	2.0897772485532996E-5
14-15	0.0	0.0	0.0	4.179554497106599E-5	2.0897772485532996E-5
16-17	0.0	0.0	0.0	4.179554497106599E-5	2.0897772485532996E-5
18-19	0.0	0.0	0.0	7.314220369936548E-5	2.0897772485532996E-5
20-21	0.0	0.0	0.0	8.359108994213198E-5	4.179554497106599E-5
22-23	0.0	0.0	0.0	1.5673329364149745E-4	4.179554497106599E-5
24-25	0.0	0.0	0.0	1.6718217988426396E-4	4.179554497106599E-5
26-27	0.0	0.0	0.0	4.597509946817259E-4	4.179554497106599E-5
28-29	0.0	0.0	0.0	0.0012120708041609136	4.179554497106599E-5
30-31	0.0	0.0	0.0	0.0048796298753719545	4.179554497106599E-5
32-33	0.0	0.0	0.0	0.014523951877445433	4.179554497106599E-5
34-35	0.0	0.0	0.0	0.030040547947953678	4.179554497106599E-5
36-37	0.0	0.0	0.0	0.05815850082723832	4.179554497106599E-5
38-39	0.0	0.0	0.0	0.1046978401525203	4.179554497106599E-5
40-41	0.0	0.0	0.0	0.15717214686369366	4.179554497106599E-5
42-43	0.0	0.0	0.0	0.21547693209833071	4.179554497106599E-5
44-45	0.0	0.0	0.0	0.28730257613110766	5.224443121383249E-5
46-47	0.0	0.0	0.0	0.380632028051498	6.269331745659899E-5
48-49	0.0	0.0	0.0	0.4923410708729146	6.269331745659899E-5
50-51	0.0	0.0	0.0	0.6107060542309735	6.269331745659899E-5
52-53	0.0	0.0	0.0	0.7271902380653343	1.0448886242766497E-4
54-55	0.0	0.0	0.0	0.8581983737771407	1.0448886242766497E-4
56-57	0.0	0.0	0.0	1.023311674185337	1.0448886242766497E-4
58-59	0.0	0.0	0.0	1.2266261026970873	1.0448886242766497E-4
60-61	0.0	0.0	0.0	1.4370248760814337	1.0448886242766497E-4
62-63	0.0	0.0	0.0	1.6629297966500451	1.0448886242766497E-4
64-65	0.0	0.0	0.0	1.9020734560882422	1.0448886242766497E-4
66-67	0.0	0.0	0.0	2.1744236760059508	1.0448886242766497E-4
68-69	0.0	0.0	0.0	2.4830942245035157	1.0448886242766497E-4
70-71	0.0	0.0	0.0	2.8282104882158503	1.0448886242766497E-4
72-73	0.0	0.0	0.0	3.1906823519774203	1.1493774867043148E-4
74-75	0.0	0.0	0.0	3.5474909193954107	1.2538663491319798E-4
76-77	0.0	0.0	0.0	3.965226942494972	1.2538663491319798E-4
78-79	0.0	0.0	0.0	4.417350250219479	1.2538663491319798E-4
80-81	0.0	0.0	0.0	4.910725760830427	1.2538663491319798E-4
82-83	0.0	1.0448886242766498E-5	0.0	5.425416999376619	1.2538663491319798E-4
84-85	0.0	2.0897772485532996E-5	0.0	5.925615632704094	1.2538663491319798E-4
86-87	0.0	2.0897772485532996E-5	0.0	6.465896193658821	1.2538663491319798E-4
88	0.0	2.0897772485532996E-5	0.0	6.896808262310512	1.2538663491319798E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5075	0.0	36.751205	94
TATTCTC	2315	0.0	33.485054	5
GTACTAG	5390	0.0	33.085484	1
CAACGCA	9945	0.0	32.217926	5
GTATCAA	11325	0.0	32.118156	1
ATCAACG	10600	0.0	30.409822	3
CGTTAAC	1165	0.0	29.96679	1
TAGGCAT	6575	0.0	29.653116	5
AGGGCTA	5605	0.0	29.336645	5
GCATCGT	5860	0.0	27.881239	8
GCTATTG	5935	0.0	27.608013	8
TATCAAC	13280	0.0	27.386911	2
CTATTGA	6040	0.0	27.286688	9
CTGTGCG	7440	0.0	27.137896	9
CTAACGC	2750	0.0	26.99728	3
TAGTACT	7630	0.0	26.971153	4
GGTATCA	7590	0.0	26.72767	1
CTAGGCA	7770	0.0	26.545658	4
TCTAACG	2810	0.0	26.421103	2
AACGCAG	12960	0.0	26.04324	6
>>END_MODULE