##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146427_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4521534
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.485109036004154	31.0	30.0	34.0	27.0	34.0
2	30.606421625934914	31.0	31.0	34.0	26.0	34.0
3	30.810745645172634	31.0	31.0	34.0	27.0	34.0
4	34.27502458236519	37.0	35.0	37.0	30.0	37.0
5	34.460104026642284	37.0	35.0	37.0	30.0	37.0
6	34.651606512303125	37.0	35.0	37.0	31.0	37.0
7	34.547840401067425	37.0	35.0	37.0	30.0	37.0
8	34.490903308478934	37.0	35.0	37.0	30.0	37.0
9	35.878370924557906	39.0	35.0	39.0	30.0	39.0
10-11	35.80464063744738	39.0	35.0	39.0	30.0	39.0
12-13	35.780267161542966	39.0	35.0	39.0	30.0	39.0
14-15	36.7995982115804	40.0	36.0	41.0	29.5	41.0
16-17	36.62871660370131	40.0	36.0	41.0	28.5	41.0
18-19	36.579381466555375	40.0	36.0	41.0	28.0	41.0
20-21	36.90578861067947	40.0	36.0	41.0	30.0	41.0
22-23	36.6144220081061	40.0	36.0	41.0	28.5	41.0
24-25	36.71993033780129	40.0	36.0	41.0	29.5	41.0
26-27	36.781821501286956	40.0	36.0	41.0	30.0	41.0
28-29	36.61871181329168	40.0	35.5	41.0	29.5	41.0
30-31	36.55960720410374	40.0	35.0	41.0	29.5	41.0
32-33	36.661688157160825	40.0	35.0	41.0	30.0	41.0
34-35	36.70586840660714	40.0	35.0	41.0	30.0	41.0
36-37	36.636450815143704	40.0	35.0	41.0	30.0	41.0
38-39	36.46418660127293	39.0	35.0	41.0	29.0	41.0
40-41	36.42369061031057	39.0	35.0	41.0	29.0	41.0
42-43	36.2634608077701	39.0	35.0	41.0	28.0	41.0
44-45	36.075080713757764	39.0	35.0	41.0	28.0	41.0
46-47	35.92964909696576	38.5	34.5	41.0	28.0	41.0
48-49	35.78922407749228	38.0	34.0	40.0	27.5	41.0
50-51	35.56226636800696	38.0	34.0	40.0	27.0	41.0
52-53	35.36403552422695	38.0	34.0	40.0	27.0	41.0
54-55	35.15190663168739	37.5	33.5	40.0	27.0	41.0
56-57	34.989566593992215	37.0	33.0	40.0	26.5	41.0
58-59	34.709509316970745	36.5	33.0	40.0	26.0	41.0
60-61	34.45446246782619	36.0	33.0	39.5	26.0	41.0
62-63	34.178382159682975	35.5	33.0	39.0	26.0	41.0
64-65	33.884068658999354	35.0	32.5	39.0	26.0	41.0
66-67	33.59478110747371	35.0	32.0	38.5	25.5	40.5
68-69	33.300554745358546	35.0	32.0	38.0	25.0	40.0
70-71	33.2455953001791	35.0	32.0	37.0	25.0	40.0
72-73	33.04242254951528	35.0	32.0	37.0	25.0	40.0
74-75	32.7917661351214	35.0	32.0	36.0	25.0	39.0
76-77	32.57034537393725	35.0	32.0	36.0	25.0	39.0
78-79	32.358167493598415	35.0	31.0	35.5	24.0	39.0
80-81	32.15299696961252	35.0	31.0	35.0	24.0	37.5
82-83	31.9240558624573	35.0	31.0	35.0	24.0	37.0
84-85	31.763657533040778	35.0	31.0	35.0	24.0	36.5
86-87	31.612948614341946	34.5	31.0	35.0	24.0	36.0
88-89	31.63019773820124	35.0	31.0	35.0	24.0	36.0
90-91	31.55877485384385	35.0	31.0	35.0	24.0	36.0
92-93	31.416935712525884	35.0	31.0	35.0	23.0	36.0
94-95	31.405273851750312	35.0	31.0	35.0	23.0	35.0
96-97	31.321036732224066	35.0	31.0	35.0	23.0	35.0
98-99	31.247128739936493	35.0	31.0	35.0	23.0	35.0
100	30.749255894127966	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8110713295150589
1101	2	0.9739404050407501
1101	3	0.9225755107714164
1101	4	0.9443897375835988
1101	5	0.8593724831406675
1101	6	0.635963691006026
1101	7	0.6804022033246753
1101	8	0.7418347992123557
1101	9	0.8953220998336349
1101	10-11	0.9626588840086256
1101	12-13	0.872874934421553
1101	14-15	1.0575524878558369
1101	16-17	1.1878074832739145
1101	18-19	1.2897933090826115
1101	20-21	0.9614990957767304
1101	22-23	1.2927115143963022
1101	24-25	1.1309275895640596
1101	26-27	1.1353706308548368
1101	28-29	1.2881114077770945
1101	30-31	1.286876627934241
1101	32-33	1.1188822535884029
1101	34-35	0.9843164144349643
1101	36-37	0.9872109866049357
1101	38-39	1.0836247260218457
1101	40-41	1.0074821259089362
1101	42-43	0.9631295853307975
1101	44-45	1.0269960967620761
1101	46-47	1.072964053471793
1101	48-49	1.0043922161486663
1101	50-51	1.0327819788189387
1101	52-53	0.9580517057146878
1101	54-55	0.9555254949562766
1101	56-57	0.8361669771165054
1101	58-59	0.823584989082299
1101	60-61	0.8688147200958554
1101	62-63	0.8328935801956447
1101	64-65	0.821756570658593
1101	66-67	0.8216283406810945
1101	68-69	0.8481611241612725
1101	70-71	0.8208548895223586
1101	72-73	0.7461274665394768
1101	74-75	0.708833699344936
1101	76-77	0.7392130440734306
1101	78-79	0.7259579769238584
1101	80-81	0.7591410370102665
1101	82-83	0.7192386653159275
1101	84-85	0.7642813896664578
1101	86-87	0.7667102433766217
1101	88-89	0.7400427534913661
1101	90-91	0.7543934547676834
1101	92-93	0.7883133380600569
1101	94-95	0.7260845793341986
1101	96-97	0.663561502865349
1101	98-99	0.7143177789606803
1101	100	0.7918655505888843
1102	1	0.6998950309793379
1102	2	0.792589887893044
1102	3	0.7576388501156401
1102	4	0.8733181768741574
1102	5	0.8202737500942092
1102	6	0.5289863426754664
1102	7	0.5935098477110472
1102	8	0.564891995448491
1102	9	0.7544882220758211
1102	10-11	0.7345534353750836
1102	12-13	0.7220310591136467
1102	14-15	0.9162128369763778
1102	16-17	1.0761212346863687
1102	18-19	1.1041348248936984
1102	20-21	0.868514338551158
1102	22-23	1.1438907940727177
1102	24-25	1.025480653868371
1102	26-27	0.8209151192235993
1102	28-29	1.0445125793174697
1102	30-31	1.1093065138384262
1102	32-33	0.9878179967778138
1102	34-35	0.8498366891082298
1102	36-37	0.7700773752540186
1102	38-39	0.8142250206777106
1102	40-41	0.79199558958193
1102	42-43	0.8426016817705957
1102	44-45	0.9081172785687386
1102	46-47	0.8624666362937532
1102	48-49	0.8637065725389306
1102	50-51	0.9202540527595602
1102	52-53	0.8734655780393652
1102	54-55	0.8788465461010659
1102	56-57	0.7832884680785099
1102	58-59	0.818377252949297
1102	60-61	0.7716307314254394
1102	62-63	0.7083362827132191
1102	64-65	0.7122973341639351
1102	66-67	0.7749308237567192
1102	68-69	0.7568972725858316
1102	70-71	0.6504862926209114
1102	72-73	0.6272064728092488
1102	74-75	0.6394416187212215
1102	76-77	0.6650856787434947
1102	78-79	0.5725036764982079
1102	80-81	0.661189586896775
1102	82-83	0.6327825124582063
1102	84-85	0.6093563619174702
1102	86-87	0.5746361298441229
1102	88-89	0.6280598803633808
1102	90-91	0.6358182191929878
1102	92-93	0.6165023461297636
1102	94-95	0.6306514431325283
1102	96-97	0.635296862152174
1102	98-99	0.6596942714059573
1102	100	0.6783764826880407
1103	1	0.6043301964578198
1103	2	0.7829772532939074
1103	3	0.7677955914765242
1103	4	0.8136056502946545
1103	5	0.7583975176527176
1103	6	0.5047634680050734
1103	7	0.6239869436878465
1103	8	0.6475525171692453
1103	9	0.7566579817493206
1103	10-11	0.8480815239151767
1103	12-13	0.6525964487042302
1103	14-15	0.8872909263181938
1103	16-17	1.1324567877233207
1103	18-19	1.1808759256271628
1103	20-21	0.8807083715545829
1103	22-23	1.123398038767121
1103	24-25	0.9779632033476418
1103	26-27	0.8828412956100635
1103	28-29	1.0691357520480906
1103	30-31	1.130994314033849
1103	32-33	0.9475232751451657
1103	34-35	0.8877692789353375
1103	36-37	0.8394373458488573
1103	38-39	0.9206548311508698
1103	40-41	0.7318147822750802
1103	42-43	0.7926430317574713
1103	44-45	0.9038843042591509
1103	46-47	0.8788964762178892
1103	48-49	0.8599811776013553
1103	50-51	0.9027365821958142
1103	52-53	0.9154153831066338
1103	54-55	0.9191839716473638
1103	56-57	0.7767378861108796
1103	58-59	0.8136820210457714
1103	60-61	0.7983985180145083
1103	62-63	0.7525043904324562
1103	64-65	0.7680591351695867
1103	66-67	0.8969435950083806
1103	68-69	0.9092882745276398
1103	70-71	0.8225257374569424
1103	72-73	0.7655726852927955
1103	74-75	0.7103525615091044
1103	76-77	0.7325558682212545
1103	78-79	0.7205020859819413
1103	80-81	0.7061557444542501
1103	82-83	0.6668350999554065
1103	84-85	0.6922205555366858
1103	86-87	0.7306859654749616
1103	88-89	0.714870525074673
1103	90-91	0.6811482599073209
1103	92-93	0.7606522596911489
1103	94-95	0.7113957187628941
1103	96-97	0.7164114019839616
1103	98-99	0.7364775033952142
1103	100	0.7832508112876226
1104	1	0.6598636770507831
1104	2	0.8226224879413522
1104	3	0.7674022753342378
1104	4	0.8522242781859646
1104	5	0.776526439521227
1104	6	0.4632182001887202
1104	7	0.5561858874452525
1104	8	0.632659499805257
1104	9	0.7555885425749054
1104	10-11	0.7019848236407924
1104	12-13	0.6913905604486104
1104	14-15	0.8663473660553223
1104	16-17	1.0028027752533575
1104	18-19	0.9570604880169782
1104	20-21	0.8103195435398831
1104	22-23	1.0478040233029233
1104	24-25	0.8700313977200267
1104	26-27	0.8249924445191112
1104	28-29	0.9804539725555088
1104	30-31	0.9716573146972252
1104	32-33	0.8334928107288206
1104	34-35	0.7493098166330299
1104	36-37	0.8214226437624461
1104	38-39	0.8763370717170886
1104	40-41	0.7592863741374671
1104	42-43	0.7218508853031835
1104	44-45	0.7774880561531319
1104	46-47	0.715449419990783
1104	48-49	0.7085745077468673
1104	50-51	0.8047729127981995
1104	52-53	0.7506694801766756
1104	54-55	0.7175119263259901
1104	56-57	0.6258765834503919
1104	58-59	0.6486644831467459
1104	60-61	0.7219456359332028
1104	62-63	0.6134060313899852
1104	64-65	0.5857021712475472
1104	66-67	0.6238544209787307
1104	68-69	0.57797879312416
1104	70-71	0.5554562524655466
1104	72-73	0.5829695761270699
1104	74-75	0.5236569801368631
1104	76-77	0.617390506047748
1104	78-79	0.5684536961582225
1104	80-81	0.5813361084604338
1104	82-83	0.5547103958298152
1104	84-85	0.5830498766581869
1104	86-87	0.514948350900255
1104	88-89	0.4548735112444007
1104	90-91	0.5555884539131775
1104	92-93	0.503070558668206
1104	94-95	0.43864271065264404
1104	96-97	0.4950527786485388
1104	98-99	0.6085905500046316
1104	100	0.7247803658175656
1105	1	0.52524987144335
1105	2	0.7054317856826167
1105	3	0.6628250087869851
1105	4	0.7158457946385752
1105	5	0.6103683933945803
1105	6	0.34972935493500046
1105	7	0.4324162689620792
1105	8	0.4594662889141219
1105	9	0.4952570433226171
1105	10-11	0.5391730218377759
1105	12-13	0.4610811333417004
1105	14-15	0.6710493214924966
1105	16-17	0.8177037831953555
1105	18-19	0.8684107029271786
1105	20-21	0.6169342944551133
1105	22-23	0.9019542608051836
1105	24-25	0.756688058205981
1105	26-27	0.6615837039397405
1105	28-29	0.9060673615810941
1105	30-31	0.8572483818241992
1105	32-33	0.7769769204654864
1105	34-35	0.6373395485302851
1105	36-37	0.5340019587758604
1105	38-39	0.6351738264791749
1105	40-41	0.5927864476759197
1105	42-43	0.6261155154962026
1105	44-45	0.6868677507588075
1105	46-47	0.5896649622482926
1105	48-49	0.6272180065642914
1105	50-51	0.680618849827404
1105	52-53	0.6545141005329995
1105	54-55	0.6059388556329353
1105	56-57	0.617872645511298
1105	58-59	0.5776159954351741
1105	60-61	0.6740087689780623
1105	62-63	0.625487945714184
1105	64-65	0.5613876540719573
1105	66-67	0.679538331010761
1105	68-69	0.6727018461794216
1105	70-71	0.5502572636233154
1105	72-73	0.45651170288515885
1105	74-75	0.5205854235523333
1105	76-77	0.5408605267642059
1105	78-79	0.5370211304270001
1105	80-81	0.6288837154246067
1105	82-83	0.5414684961186396
1105	84-85	0.4880587469786377
1105	86-87	0.5355386679513643
1105	88-89	0.5019231217028235
1105	90-91	0.5654903327267071
1105	92-93	0.5916156065596141
1105	94-95	0.5555550319260512
1105	96-97	0.5709181980617437
1105	98-99	0.5477111811391957
1105	100	0.6553406847312147
1106	1	0.533743786921022
1106	2	0.5936252364096823
1106	3	0.5777657630536126
1106	4	0.690308843341036
1106	5	0.5558367220835123
1106	6	0.31322365229436855
1106	7	0.3382784172666362
1106	8	0.3798688856459833
1106	9	0.5462246114487357
1106	10-11	0.5349979576504396
1106	12-13	0.39263524192149646
1106	14-15	0.5988271549323585
1106	16-17	0.7172882319074887
1106	18-19	0.7308631709822038
1106	20-21	0.5226066847351021
1106	22-23	0.8138135419555468
1106	24-25	0.6068224264883924
1106	26-27	0.5096285372872487
1106	28-29	0.7110989877859879
1106	30-31	0.6498904619896138
1106	32-33	0.5820684529934752
1106	34-35	0.5185145863576466
1106	36-37	0.429910274409643
1106	38-39	0.5467601944201306
1106	40-41	0.5398085438155533
1106	42-43	0.5422857956065172
1106	44-45	0.5336448104558826
1106	46-47	0.5223540506380573
1106	48-49	0.5720215211799058
1106	50-51	0.6248258060061147
1106	52-53	0.6116968736413284
1106	54-55	0.6187581527567261
1106	56-57	0.5040572216339569
1106	58-59	0.5190736817712676
1106	60-61	0.5194260237246269
1106	62-63	0.5192364629655941
1106	64-65	0.5375459811462804
1106	66-67	0.570608577401778
1106	68-69	0.4905670726770097
1106	70-71	0.4282067529813034
1106	72-73	0.4066130953558087
1106	74-75	0.5317635140476824
1106	76-77	0.46485823841621965
1106	78-79	0.4470642470761774
1106	80-81	0.5147453023372321
1106	82-83	0.5252986791964105
1106	84-85	0.5128740094756807
1106	86-87	0.5276228231453786
1106	88-89	0.5564620489647609
1106	90-91	0.5753924493531777
1106	92-93	0.6179522133406294
1106	94-95	0.5638670019509036
1106	96-97	0.5386954347245023
1106	98-99	0.617710422544441
1106	100	0.6261513660793128
1107	1	0.4403519224268635
1107	2	0.6025373560502132
1107	3	0.516906188452456
1107	4	0.6435923664429595
1107	5	0.5933392779180693
1107	6	0.2877563829526082
1107	7	0.36042309167920905
1107	8	0.2859251848279314
1107	9	0.42760845435140027
1107	10-11	0.45276363817785636
1107	12-13	0.3853750821766866
1107	14-15	0.5790973817761014
1107	16-17	0.7433738202636988
1107	18-19	0.8408074158985954
1107	20-21	0.5365482349130986
1107	22-23	0.7822608570126022
1107	24-25	0.49564081373308966
1107	26-27	0.45863936282871265
1107	28-29	0.624385314557955
1107	30-31	0.6205920701189243
1107	32-33	0.5932309067370198
1107	34-35	0.47936001064297784
1107	36-37	0.4462281658834186
1107	38-39	0.4900011622017644
1107	40-41	0.4284809590655243
1107	42-43	0.5091904123205069
1107	44-45	0.45328834206598856
1107	46-47	0.36922055047616453
1107	48-49	0.3815725964619432
1107	50-51	0.4245867402492749
1107	52-53	0.4771679695867803
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2214	3	-1.0869719775478828
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2214	90-91	-0.8421175132126741
2214	92-93	-0.8596570576319422
2214	94-95	-1.0209689308154708
2214	96-97	-0.9347481775981841
2214	98-99	-1.0940524827662124
2214	100	-0.9785286565752784
2215	1	-4.088347191913183
2215	2	-2.2132202950970665
2215	3	-3.7644301450585154
2215	4	-1.9618230201578015
2215	5	-1.5637092284552807
2215	6	-1.369191511425413
2215	7	-1.284858633964518
2215	8	-1.2058635043715569
2215	9	-1.8086017778220054
2215	10-11	-1.4266840460375079
2215	12-13	-1.347755425134764
2215	14-15	-3.7848236264991115
2215	16-17	-3.520042539862054
2215	18-19	-2.366972298544603
2215	20-21	-1.8834868894828247
2215	22-23	-3.1722923513085277
2215	24-25	-5.535309223763591
2215	26-27	-3.647361028477121
2215	28-29	-1.748277919881545
2215	30-31	-4.052510701541991
2215	32-33	-4.089017557662004
2215	34-35	-3.078589359737961
2215	36-37	-1.9941807628407986
2215	38-39	-1.8097176064837015
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2215	42-43	-1.772257030924969
2215	44-45	-3.352870051647706
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2215	50-51	-5.160662905135016
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2215	54-55	-3.2369187997793745
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2215	62-63	-2.8401857803526944
2215	64-65	-1.8874643629028753
2215	66-67	-1.68889965051725
2215	68-69	-1.8128269441680729
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2215	90-91	-1.7636717454000923
2215	92-93	-4.152732790742398
2215	94-95	-2.258746232621938
2215	96-97	-1.7576431364797855
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2215	100	-3.277361566435861
2216	1	-0.6625815223954348
2216	2	-0.6174349714164897
2216	3	-0.8548910544532546
2216	4	-0.7885958172029817
2216	5	-0.8907368593192828
2216	6	-0.7990341719177181
2216	7	-0.9823051839277639
2216	8	-0.8142538140029103
2216	9	-1.0223723780561187
2216	10-11	-1.057903680577688
2216	12-13	-1.2101206638333935
2216	14-15	-1.618829066766402
2216	16-17	-1.3509619933517598
2216	18-19	-3.269111346504438
2216	20-21	-1.5666381005688308
2216	22-23	-0.9208565793316765
2216	24-25	-1.1689679958921317
2216	26-27	-1.1738166244282056
2216	28-29	-1.1626328853207468
2216	30-31	-1.3235321185144997
2216	32-33	-1.177902829224287
2216	34-35	-1.4988600255041007
2216	36-37	-1.5593304359373121
2216	38-39	-1.5756791006482302
2216	40-41	-1.6374758554586109
2216	42-43	-1.6374670677944678
2216	44-45	-1.5162211846399174
2216	46-47	-1.6035850206725115
2216	48-49	-1.6708466445882237
2216	50-51	-1.460424202427248
2216	52-53	-1.588411354072882
2216	54-55	-1.485475626514976
2216	56-57	-1.5367400016027943
2216	58-59	-1.568515245324548
2216	60-61	-1.5276172217282706
2216	62-63	-1.5496471486856152
2216	64-65	-1.6137245045633293
2216	66-67	-1.5628056264276822
2216	68-69	-1.6654679414189175
2216	70-71	-1.6408106138005785
2216	72-73	-1.6190463081771398
2216	74-75	-1.6947812777279125
2216	76-77	-1.470802456642346
2216	78-79	-1.6749329488370357
2216	80-81	-1.7005052353915033
2216	82-83	-1.8222947303520876
2216	84-85	-1.6708114961212388
2216	86-87	-1.6865728844655692
2216	88-89	-1.600530271312941
2216	90-91	-1.67773877060419
2216	92-93	-1.5497549425139638
2216	94-95	-1.4978440679952811
2216	96-97	-1.496259557487896
2216	98-99	-1.429494249578962
2216	100	-1.4735049062477046
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	18511.0
1	3235.0
2	552.0
3	473.0
4	412.0
5	269.0
6	215.0
7	286.0
8	507.0
9	911.0
10	1615.0
11	3159.0
12	5271.0
13	7643.0
14	9955.0
15	12510.0
16	15207.0
17	17695.0
18	20752.0
19	23518.0
20	26961.0
21	30896.0
22	35764.0
23	40877.0
24	46402.0
25	52461.0
26	59781.0
27	69555.0
28	82190.0
29	97339.0
30	115672.0
31	139602.0
32	171229.0
33	218071.0
34	303074.0
35	408463.0
36	606337.0
37	919449.0
38	716975.0
39	237547.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73777641166498	21.083158666232112	12.282237612336653	23.89682730976625
2	15.501294758936679	19.735431962506198	40.6452778177669	24.11799546079022
3	18.81364787865036	26.68208857604543	28.607145563697163	25.89711798160705
4	12.161180251750539	16.467866342186614	40.09418869024658	31.27676471581627
5	13.108720806380209	38.61684567296126	34.993026118236784	13.281407402421744
6	29.91862629703646	38.389634553975924	18.117127993229452	13.574611155758165
7	27.04424198856662	31.514053434303424	23.86707620192062	17.574628375209336
8	26.240903471935557	34.976682522862326	19.87766299282192	18.904751012380192
9	26.07922442131961	14.940530104453051	21.719973694425807	37.260271779801535
10-11	26.04190604520801	25.610882919781712	27.27957579204071	21.06763524296957
12-13	26.124553548300167	23.358915481743146	27.381566756133036	23.134964213823654
14-15	23.464104764189635	24.579282093060332	24.841872946493947	27.114740196256093
16-17	22.341030742385556	27.226414559426587	26.237741930283608	24.194812767904246
18-19	21.70794375093735	28.04288397905408	27.81779789858141	22.431374371427165
20-21	23.58718600087828	27.18759067688733	26.56238847785122	22.662834844383177
22-23	24.099751097899752	26.68970208540108	26.360918380626064	22.849628436073104
24-25	23.558337426317937	26.74027072174901	27.103836703366046	22.597555148567004
26-27	23.422530220489868	26.965515585632165	26.03305858611367	23.578895607764295
28-29	22.34777492638999	27.713755248072037	26.72026885423823	23.218200971299737
30-31	23.276778945449454	27.16453647485677	26.627045617748212	22.931638961945563
32-33	23.735878600681005	27.356188137329806	25.586290897129814	23.321642364859375
34-35	23.755465273231145	27.134570877869052	26.626885670047194	22.48307817885261
36-37	23.334228093589104	26.699742402451303	26.247762450714983	23.71826705324461
38-39	23.52604158038322	26.79563051187907	26.919167093706015	22.75916081403169
40-41	23.102496684604144	25.881969794900776	27.588863561133763	23.426669959361313
42-43	22.975262995852795	26.17750081083879	26.818344203261525	24.02889199004689
44-45	23.25780751429988	27.025999490487333	26.723483296335655	22.992709698877125
46-47	23.245003332557182	26.88030393099416	26.176041304583276	23.698651431865382
48-49	23.995779696230173	26.440752277706746	26.46256303525706	23.100904990806022
50-51	23.458966268639227	26.240514656496337	26.57533890184096	23.72518017302347
52-53	22.97987723227314	26.915748521267567	26.292938270946138	23.81143597551316
54-55	22.871546583699693	26.775672187803984	26.3186392948	24.03414193369632
56-57	24.12272402957408	26.12617610287472	25.99519475665507	23.75590511089613
58-59	22.957702243046864	26.627869680817064	26.922677114318066	23.491750961818006
60-61	22.899491004333996	27.317431086589295	26.320384339809223	23.46269356926749
62-63	23.376342723364804	26.544564019960625	25.809491413129148	24.269601843545423
64-65	22.44447834065499	26.8326577932218	26.324129400414332	24.398734465708884
66-67	23.350383998500494	26.337446638155136	26.204738944277334	24.10743041906704
68-69	22.575857585803057	26.472183587389175	26.58960778095406	24.362351045853703
70-71	22.648941873473092	26.122349235898184	26.68620605388948	24.542502836739235
72-73	23.26869368750847	26.23463176326664	27.14451521043918	23.35215933878571
74-75	23.491429054271908	26.651205091201803	26.58894371349093	23.268422141035362
76-77	23.226975209463323	26.552253023709994	26.121926223243676	24.09884554358301
78-79	23.086666100276805	26.554895854693424	26.198237044832723	24.160201000197052
80-81	22.676336996727937	26.914999728736255	26.95714566507199	23.451517609463814
82-83	23.580165648313784	25.86627068727897	28.04125943946475	22.5123042249425
84-85	23.793119243716745	26.498154053533003	26.48105433862461	23.22767236412564
86-87	22.359227694783172	27.225525426594405	27.01717708404662	23.398069794575804
88-89	23.489145928226066	25.813301981387653	26.797806452204476	23.899745638181805
90-91	23.14029700952154	25.965607434753203	26.893509215900185	24.000586339825077
92-93	23.498351842424487	26.253689360564504	26.897768387598624	23.350190409412384
94-95	22.250288376944336	26.255731198248135	27.642702419593263	23.851278005214265
96-97	22.664098349493212	26.187141367054203	27.119054537867825	24.029705745584764
98-99	22.01939865792762	26.98910705195653	26.855579582226614	24.135914707889235
100	22.92497745591858	26.106281061697107	25.97355438942712	24.99518709295719
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15535.0
1	9102.0
2	2441.0
3	1736.0
4	909.0
5	490.0
6	399.0
7	346.0
8	335.0
9	325.0
10	278.0
11	269.5
12	278.0
13	288.0
14	316.5
15	397.0
16	462.5
17	521.0
18	643.0
19	816.0
20	1037.5
21	1328.5
22	1782.0
23	2412.0
24	3265.5
25	4467.5
26	6381.5
27	8537.0
28	12066.0
29	20027.0
30	31676.5
31	43144.0
32	58041.0
33	72469.5
34	83984.5
35	97016.5
36	109602.5
37	131589.0
38	140814.0
39	131032.0
40	121313.0
41	111445.0
42	109765.0
43	118443.0
44	131705.5
45	159590.0
46	188463.5
47	207848.5
48	222060.0
49	217855.5
50	206254.0
51	212028.5
52	216267.0
53	207975.0
54	197403.5
55	170898.0
56	148785.0
57	123666.0
58	97445.0
59	92433.0
60	77042.0
61	53787.5
62	40656.0
63	33955.0
64	25180.0
65	12807.0
66	7131.5
67	5031.5
68	3633.0
69	2683.5
70	2160.0
71	1703.0
72	1290.5
73	1098.5
74	921.5
75	669.0
76	480.0
77	301.5
78	147.5
79	87.5
80	59.5
81	47.0
82	40.5
83	32.0
84	28.0
85	21.5
86	16.0
87	13.5
88	10.0
89	8.5
90	8.0
91	6.0
92	4.5
93	4.0
94	4.5
95	4.5
96	3.0
97	1.5
98	2.0
99	3.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9935344951514243
2	0.4491838389360779
3	0.4837959860525211
4	0.4690001225247892
5	0.4365111486499935
6	0.4875778883892059
7	0.5037228515809016
8	0.5081682455556013
9	0.5225660141005243
10-11	0.5354709264599139
12-13	0.5336020916795052
14-15	0.5317443150930636
16-17	0.5361344180979287
18-19	0.5339670120804134
20-21	0.5332039966966964
22-23	0.4948652382134028
24-25	0.5308154267998427
26-27	0.510800095719727
28-29	0.5160748542419453
30-31	0.5198235819967294
32-33	0.5223337920272191
34-35	0.5343429906752885
36-37	0.5525779525267309
38-39	0.5415308167537831
40-41	0.5465070040388947
42-43	0.5451136715990635
44-45	0.5546900675744117
46-47	0.5526221853025987
48-49	0.5253416207862199
50-51	0.53960669100354
52-53	0.5311582308128171
54-55	0.5414865839779155
56-57	0.529621141851416
58-59	0.5350285987012373
60-61	0.5297206655971182
62-63	0.5236939498851496
64-65	0.5340001866623142
66-67	0.5320207699422365
68-69	0.5532635605526798
70-71	0.5372734120765209
72-73	0.541287536486511
74-75	0.5388436756198228
76-77	0.5261378107518377
78-79	0.5577753036911809
80-81	0.5322529920155417
82-83	0.5306495538903391
84-85	0.5392638869905656
86-87	0.8443373421498103
88-89	1.1090815639117169
90-91	1.099726331815707
92-93	1.099682099039839
94-95	1.1048462756223885
96-97	1.0970723652636472
98-99	1.0930029498838227
100	1.0877945405254057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4521534.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.89514650981984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.48957857119886	19.7651298261731
2	12.395596231908069	6.6676275346747635
3	5.1472537811973105	4.153084337055774
4	2.615100562928923	2.813340511115429
5	1.5976332812513254	2.14842905841093
6	1.0022375685490093	1.6173195746262727
7	0.6462026923047735	1.216580125920384
8	0.5008892962386798	1.0777192806031877
9	0.3381037296245062	0.8184014409390847
>10	1.7354482707919898	8.530757507494831
>50	0.19841725403152016	3.81035464663786
>100	0.24242558957495217	14.766136194383122
>500	0.05094765354822624	9.668658453198463
>1k	0.037696325242349116	17.76394847901659
>5k	0.0018930469008166587	3.125530161084279
>10k+	5.761447089442004E-4	2.0569828686660516
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	18368	0.40623381356858096	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	15913	0.35193808119102943	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	13947	0.30845726251312056	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12737	0.2816964331131868	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11343	0.25086618833342844	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10462	0.23138165056372462	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10153	0.2245476866921713	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8701	0.19243469141225078	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	8556	0.1892278151618455	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	7752	0.17144623926304656	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7248	0.1602995797443965	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7162	0.15839757038208716	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6458	0.14282763327667117	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6415	0.14187662859551647	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6300	0.13933324398312608	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6278	0.1388466834485818	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6230	0.13778509682775802	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6087	0.1346224533532204	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6057	0.13395896171520552	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5673	0.12546626874861494	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5525	0.1221930433344082	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5512	0.1219055302912684	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5274	0.11664182996301699	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5219	0.11542542862665636	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5219	0.11542542862665636	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5195	0.11489463531624444	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	5190	0.1147840533765753	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5068	0.11208585404864808	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5060	0.11190892294517746	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5015	0.11091368548815514	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	4973	0.10998479719493427	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4906	0.10850299920336771	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4698	0.10390279051313116	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	4651	0.10286332028024117	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4635	0.1025094580732999	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4590	0.10151422061627757	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4527	0.10012088817644631	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.1058193966914768E-5	0.0
12-13	0.0	0.0	0.0	4.423277586765907E-5	0.0
14-15	0.0	0.0	0.0	6.63491638014886E-5	0.0
16-17	0.0	0.0	0.0	7.740735776840338E-5	0.0
18-19	0.0	0.0	0.0	8.846555173531815E-5	0.0
20-21	0.0	2.2116387933829537E-5	0.0	1.1058193966914767E-4	0.0
22-23	0.0	2.2116387933829537E-5	0.0	1.8798929743755105E-4	0.0
24-25	0.0	2.2116387933829537E-5	0.0	3.3174581900744307E-4	0.0
26-27	0.0	2.2116387933829537E-5	0.0	4.3126956470967596E-4	0.0
28-29	0.0	2.2116387933829537E-5	0.0	0.0012606341122282836	0.0
30-31	0.0	2.2116387933829537E-5	0.0	0.005518038789490469	0.0
32-33	0.0	2.2116387933829537E-5	0.0	0.016388243458967687	0.0
34-35	0.0	2.2116387933829537E-5	0.0	0.03322987287057888	0.0
36-37	0.0	2.2116387933829537E-5	0.0	0.06430339791760938	0.0
38-39	0.0	2.2116387933829537E-5	0.0	0.1146402968550054	0.0
40-41	0.0	2.2116387933829537E-5	0.0	0.17015243056891755	0.0
42-43	0.0	2.2116387933829537E-5	0.0	0.23023159839116547	0.0
44-45	0.0	2.2116387933829537E-5	0.0	0.30299451469346467	0.0
46-47	0.0	2.2116387933829537E-5	0.0	0.39711080354587625	0.0
48-49	0.0	2.2116387933829537E-5	0.0	0.5108885612714623	0.0
50-51	0.0	2.2116387933829537E-5	0.0	0.6329710226662013	0.0
52-53	0.0	2.2116387933829537E-5	0.0	0.7519240151683035	0.0
54-55	0.0	2.2116387933829537E-5	0.0	0.887950859155322	0.0
56-57	0.0	2.2116387933829537E-5	0.0	1.0579705913966366	0.0
58-59	0.0	2.2116387933829537E-5	0.0	1.2648804587115787	0.0
60-61	0.0	2.2116387933829537E-5	0.0	1.4770982591306403	0.0
62-63	0.0	2.2116387933829537E-5	0.0	1.7038244100342936	0.0
64-65	0.0	2.2116387933829537E-5	0.0	1.9453685408536128	0.0
66-67	0.0	2.2116387933829537E-5	0.0	2.222110903069622	0.0
68-69	0.0	2.2116387933829537E-5	0.0	2.532857211733894	0.0
70-71	0.0	2.2116387933829537E-5	0.0	2.875207838755608	0.0
72-73	0.0	2.2116387933829537E-5	0.0	3.236788665085787	0.0
74-75	0.0	2.2116387933829537E-5	0.0	3.590639813833093	0.0
76-77	0.0	2.2116387933829537E-5	0.0	3.9978578066647295	0.0
78-79	0.0	2.2116387933829537E-5	0.0	4.444431911824616	0.0
80-81	0.0	2.2116387933829537E-5	0.0	4.928050966773666	0.0
82-83	0.0	2.2116387933829537E-5	0.0	5.430557417018207	0.0
84-85	0.0	2.2116387933829537E-5	0.0	5.920358002394762	0.0
86-87	0.0	2.2116387933829537E-5	0.0	6.449459409129734	0.0
88	0.0	2.2116387933829537E-5	0.0	6.880872730360979	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5085	0.0	41.871307	94
GTACTAG	5135	0.0	34.2722	1
CTGTGCG	7290	0.0	31.234419	9
TAGTACT	7480	0.0	30.656597	4
TATTCTC	2560	0.0	30.46637	5
TAGGCAT	6490	0.0	29.392324	5
GCATCGT	5530	0.0	29.204037	8
AGGGCTA	5250	0.0	28.817024	5
ACTGTGC	8225	0.0	28.5964	8
GTACTGT	8275	0.0	27.798615	6
TACTGTG	8850	0.0	27.796669	7
GCTATTG	5310	0.0	27.761612	8
CTAGTAC	8830	0.0	27.249802	3
CTAGGCA	7425	0.0	27.149796	4
TCTAACG	2560	0.0	26.986464	2
CTATTGA	5510	0.0	26.924936	9
CTAACGC	2585	0.0	26.542778	3
GTACATG	18680	0.0	26.495508	1
TAACGCC	2640	0.0	26.164906	4
CAGTACG	650	0.0	26.002096	9
>>END_MODULE