##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146426_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4228890
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.61344584512721	31.0	31.0	34.0	27.0	34.0
2	30.7664013488173	31.0	31.0	34.0	27.0	34.0
3	30.95200087966346	31.0	31.0	34.0	27.0	34.0
4	34.515752833485855	37.0	35.0	37.0	30.0	37.0
5	34.684376278408756	37.0	35.0	37.0	32.0	37.0
6	34.84139880677908	37.0	35.0	37.0	32.0	37.0
7	34.74590093381478	37.0	35.0	37.0	32.0	37.0
8	34.67993516029029	37.0	35.0	37.0	31.0	37.0
9	36.17678989049136	39.0	35.0	39.0	31.0	39.0
10-11	36.10875347904533	39.0	35.0	39.0	30.5	39.0
12-13	36.10251780017924	39.0	35.0	39.0	30.5	39.0
14-15	37.16727261763725	40.0	36.5	41.0	31.0	41.0
16-17	36.963697447793635	40.0	36.0	41.0	30.0	41.0
18-19	36.89254721688197	40.0	36.0	41.0	30.0	41.0
20-21	37.20866693151158	40.0	36.5	41.0	31.0	41.0
22-23	36.891420443662525	40.0	36.0	41.0	30.0	41.0
24-25	36.968094582739205	40.0	36.0	41.0	30.0	41.0
26-27	37.02342446835931	40.0	36.0	41.0	30.0	41.0
28-29	36.83860031828684	40.0	36.0	41.0	30.0	41.0
30-31	36.77782786972468	40.0	36.0	41.0	30.0	41.0
32-33	36.886551080780066	40.0	36.0	41.0	30.0	41.0
34-35	36.92941670272814	40.0	36.0	41.0	30.0	41.0
36-37	36.84722906010797	40.0	36.0	41.0	30.0	41.0
38-39	36.66507653308551	39.0	35.0	41.0	30.0	41.0
40-41	36.61652159313674	39.0	35.0	41.0	30.0	41.0
42-43	36.4424774586239	39.0	35.0	41.0	29.0	41.0
44-45	36.22119362291287	39.0	35.0	41.0	28.5	41.0
46-47	36.06869414905566	38.5	35.0	40.5	28.5	41.0
48-49	35.886402578454394	38.0	34.5	40.0	28.0	41.0
50-51	35.63142798701314	38.0	34.0	40.0	28.0	41.0
52-53	35.40265483377435	38.0	34.0	40.0	27.5	41.0
54-55	35.14637895523411	37.0	34.0	40.0	27.0	41.0
56-57	34.94080657099144	36.5	33.0	40.0	27.0	41.0
58-59	34.608884600923645	36.0	33.0	39.5	26.5	41.0
60-61	34.32856517904226	35.5	33.0	39.0	26.0	41.0
62-63	34.013998708881054	35.0	33.0	39.0	26.0	40.5
64-65	33.69536828813234	35.0	32.5	38.0	26.0	40.0
66-67	33.390183476042175	35.0	32.0	37.0	26.0	40.0
68-69	33.070431129681786	35.0	32.0	37.0	25.0	40.0
70-71	33.03567614669571	35.0	32.0	36.0	26.0	39.0
72-73	32.84069826833992	35.0	32.0	36.0	25.5	39.0
74-75	32.60644388953129	35.0	32.0	35.5	25.0	39.0
76-77	32.413897736758344	35.0	32.0	35.0	25.0	37.5
78-79	32.22280302869075	35.0	32.0	35.0	25.0	37.0
80-81	32.06662528464916	35.0	31.5	35.0	24.5	37.0
82-83	31.86154593758646	35.0	31.0	35.0	24.0	36.0
84-85	31.73021301097924	34.0	31.0	35.0	24.0	36.0
86-87	31.619041876237027	34.5	31.0	35.0	24.0	36.0
88-89	31.698207212767414	35.0	32.0	35.0	24.0	36.0
90-91	31.655288385368266	35.0	32.0	35.0	24.0	35.0
92-93	31.52433439980704	35.0	32.0	35.0	24.0	35.0
94-95	31.5309578872943	35.0	32.0	35.0	24.0	35.0
96-97	31.45086240124477	35.0	31.5	35.0	24.0	35.0
98-99	31.387772441468094	35.0	31.0	35.0	23.0	35.0
100	30.866360439737143	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5209561960588012
1101	2	0.6900372894726949
1101	3	0.6584186809928987
1101	4	0.6804460389811666
1101	5	0.6619779060587092
1101	6	0.2957248361894642
1101	7	0.3306689430812071
1101	8	0.35865219189403064
1101	9	0.518635771898623
1101	10-11	0.5591027896498559
1101	12-13	0.4239838144569319
1101	14-15	0.658621305144365
1101	16-17	0.7966057974508303
1101	18-19	0.9642715232927586
1101	20-21	0.6048560647637089
1101	22-23	0.919441209101933
1101	24-25	0.774930357156677
1101	26-27	0.6549356876766481
1101	28-29	0.8693107713922146
1101	30-31	0.8619967387859546
1101	32-33	0.6938149072214941
1101	34-35	0.5757985615326859
1101	36-37	0.5541165708810212
1101	38-39	0.5751552822428252
1101	40-41	0.5277496080981123
1101	42-43	0.533677598444811
1101	44-45	0.5279515440687419
1101	46-47	0.5457600643828613
1101	48-49	0.5233548959150838
1101	50-51	0.5468459330922713
1101	52-53	0.6195771206985583
1101	54-55	0.5973125526551755
1101	56-57	0.43524078064250205
1101	58-59	0.3908338486254408
1101	60-61	0.453374347847884
1101	62-63	0.3993640147364914
1101	64-65	0.37091048374098534
1101	66-67	0.3896228831273092
1101	68-69	0.3758808473497268
1101	70-71	0.27820752142240224
1101	72-73	0.35764845889725194
1101	74-75	0.36310900103751464
1101	76-77	0.3389565038317599
1101	78-79	0.36719449778811963
1101	80-81	0.41711433745769
1101	82-83	0.3353813288475429
1101	84-85	0.31009782642304984
1101	86-87	0.2891675303448622
1101	88-89	0.30541156885480447
1101	90-91	0.34754073141724007
1101	92-93	0.3904430098402756
1101	94-95	0.3344587310823428
1101	96-97	0.273263823129259
1101	98-99	0.283872605771851
1101	100	0.4174213881981963
1102	1	0.41565931652251464
1102	2	0.6504681296100081
1102	3	0.5991733949158302
1102	4	0.6755200949928621
1102	5	0.5383101586686294
1102	6	0.2883666686234676
1102	7	0.34507445701119366
1102	8	0.3356147155333602
1102	9	0.5423454229446136
1102	10-11	0.4754463595839127
1102	12-13	0.4675714824827253
1102	14-15	0.6489381759185378
1102	16-17	0.8525724442382838
1102	18-19	0.8436514445291365
1102	20-21	0.6447014836548703
1102	22-23	0.9087912924563639
1102	24-25	0.7253419807255028
1102	26-27	0.5638145432062842
1102	28-29	0.7428863927128404
1102	30-31	0.8345293452171916
1102	32-33	0.6746755673161076
1102	34-35	0.4954267297517916
1102	36-37	0.497087792746818
1102	38-39	0.5347644960892453
1102	40-41	0.42009940208372143
1102	42-43	0.5026088263935407
1102	44-45	0.5758652044222075
1102	46-47	0.47971008223601075
1102	48-49	0.4657109110919322
1102	50-51	0.4901246391226266
1102	52-53	0.4551221947784114
1102	54-55	0.524229287373565
1102	56-57	0.39202018673564965
1102	58-59	0.29950938323076315
1102	60-61	0.42864475514284095
1102	62-63	0.39803039155761866
1102	64-65	0.3064746742781921
1102	66-67	0.3632033193162343
1102	68-69	0.38958701357518777
1102	70-71	0.3521405394533659
1102	72-73	0.3111728233227211
1102	74-75	0.3903959562244381
1102	76-77	0.33567667274306245
1102	78-79	0.360234629477155
1102	80-81	0.40069635158861416
1102	82-83	0.4283347452349737
1102	84-85	0.3958272718217266
1102	86-87	0.30884365655924384
1102	88-89	0.29366066821223313
1102	90-91	0.338302381610859
1102	92-93	0.3971087421364565
1102	94-95	0.40299361432655445
1102	96-97	0.3420825929161033
1102	98-99	0.4073460899106749
1102	100	0.46452748549000233
1103	1	0.5612566021844394
1103	2	0.6297034764229572
1103	3	0.5578746078163448
1103	4	0.4857187675982857
1103	5	0.48185788982492994
1103	6	0.3856112070329871
1103	7	0.4053710035775495
1103	8	0.42997102806502596
1103	9	0.4842756913295929
1103	10-11	0.6066216137194687
1103	12-13	0.48538361227411997
1103	14-15	0.5847528347801259
1103	16-17	0.8390006400499388
1103	18-19	0.9546724011748182
1103	20-21	0.6266146074145098
1103	22-23	0.8069035731994987
1103	24-25	0.7219007444668364
1103	26-27	0.5613390008337049
1103	28-29	0.7997028658713887
1103	30-31	0.8427056720815642
1103	32-33	0.6776474368675665
1103	34-35	0.6357987635697029
1103	36-37	0.5207094593239958
1103	38-39	0.6274834203436654
1103	40-41	0.572668038778744
1103	42-43	0.5931421316283974
1103	44-45	0.6921718618896264
1103	46-47	0.6141634752258582
1103	48-49	0.6249233384003006
1103	50-51	0.6238075067981299
1103	52-53	0.6760266472185776
1103	54-55	0.6086952450191419
1103	56-57	0.5186787549152712
1103	58-59	0.5220200735336746
1103	60-61	0.5372836723871259
1103	62-63	0.5469282658909904
1103	64-65	0.46073952397874507
1103	66-67	0.5636631474375449
1103	68-69	0.4980030982794901
1103	70-71	0.48244836162143656
1103	72-73	0.41847205548724276
1103	74-75	0.40464673806260265
1103	76-77	0.4509489626440697
1103	78-79	0.46888397502360135
1103	80-81	0.4909490030723731
1103	82-83	0.35232283007210796
1103	84-85	0.4470633825491923
1103	86-87	0.5338447350815656
1103	88-89	0.5509283102769018
1103	90-91	0.5380470330453733
1103	92-93	0.5786610652631943
1103	94-95	0.5992543024310351
1103	96-97	0.6429894620739169
1103	98-99	0.5800038885892
1103	100	0.5738283619743143
1104	1	0.3576824146274582
1104	2	0.5407177811096417
1104	3	0.4926375680727446
1104	4	0.5139554368022061
1104	5	0.48002023979888264
1104	6	0.2600095954173156
1104	7	0.22621615355335223
1104	8	0.32771248825932275
1104	9	0.37786051064383486
1104	10-11	0.3549224136007325
1104	12-13	0.33832914500349887
1104	14-15	0.47346518622283185
1104	16-17	0.6165556760139239
1104	18-19	0.6264703122380837
1104	20-21	0.46538114835192346
1104	22-23	0.6945389889589251
1104	24-25	0.5259579219852384
1104	26-27	0.4077508214100405
1104	28-29	0.5976331518465869
1104	30-31	0.6400951968889075
1104	32-33	0.44045844668866607
1104	34-35	0.3672365038858132
1104	36-37	0.3573946392315719
1104	38-39	0.499797716320856
1104	40-41	0.41624937945209695
1104	42-43	0.30341587232174305
1104	44-45	0.4330562509346336
1104	46-47	0.40541209085562
1104	48-49	0.3431208561452337
1104	50-51	0.4733464451581568
1104	52-53	0.4680209803837272
1104	54-55	0.41398643818266123
1104	56-57	0.3650269292472359
1104	58-59	0.3758458312460746
1104	60-61	0.38682209539295087
1104	62-63	0.25971404912373686
1104	64-65	0.31248108621316817
1104	66-67	0.3933849128555238
1104	68-69	0.3615144229304761
1104	70-71	0.2636916330280883
1104	72-73	0.21710090408802785
1104	74-75	0.27445785559272906
1104	76-77	0.2904942037860323
1104	78-79	0.2589456268472077
1104	80-81	0.2871301253386136
1104	82-83	0.24316343977735855
1104	84-85	0.2738772103270968
1104	86-87	0.316332432288295
1104	88-89	0.21698435092391577
1104	90-91	0.20750745203865506
1104	92-93	0.18197314367124307
1104	94-95	0.1278316723002355
1104	96-97	0.1995374237707317
1104	98-99	0.2898801365838253
1104	100	0.3991360670865767
1105	1	0.32497567997207
1105	2	0.512547716120217
1105	3	0.4635380109792244
1105	4	0.5285424196990007
1105	5	0.421284951318718
1105	6	0.1954562454585016
1105	7	0.19328434285771579
1105	8	0.2762543500903831
1105	9	0.31588967700431425
1105	10-11	0.3786975348874222
1105	12-13	0.33354340146377126
1105	14-15	0.4929743971257565
1105	16-17	0.6440568335111436
1105	18-19	0.6515679865037214
1105	20-21	0.49477525701226455
1105	22-23	0.7633382596714497
1105	24-25	0.5899036180226176
1105	26-27	0.38008573866890316
1105	28-29	0.718203817244337
1105	30-31	0.6694844751502131
1105	32-33	0.5234113004524517
1105	34-35	0.4563550536972443
1105	36-37	0.3534730746344934
1105	38-39	0.43583896792863186
1105	40-41	0.33249796823046296
1105	42-43	0.38134557357184207
1105	44-45	0.4301055451568132
1105	46-47	0.3817783358216005
1105	48-49	0.4402740039467119
1105	50-51	0.5622702373913882
1105	52-53	0.47291139969833296
1105	54-55	0.42961601646313596
1105	56-57	0.41027738800667635
1105	58-59	0.3993510668333684
1105	60-61	0.37771124338763684
1105	62-63	0.3871600681298162
1105	64-65	0.3225383367192052
1105	66-67	0.341731862946709
1105	68-69	0.42195525336856576
1105	70-71	0.3605838365071108
1105	72-73	0.30303514180224056
1105	74-75	0.3374446660340027
1105	76-77	0.3053759437985377
1105	78-79	0.33202709769344096
1105	80-81	0.37461959738114103
1105	82-83	0.4097362048570652
1105	84-85	0.3914948575219448
1105	86-87	0.4066461461508766
1105	88-89	0.38685268144037366
1105	90-91	0.4015205835298232
1105	92-93	0.4259492506676672
1105	94-95	0.34171290306535695
1105	96-97	0.3367375086624236
1105	98-99	0.45419916080210854
1105	100	0.4063722103932115
1106	1	0.3509776013710031
1106	2	0.3945808570171465
1106	3	0.40341979561595664
1106	4	0.5165920420907355
1106	5	0.4890189742315556
1106	6	0.17272163221685588
1106	7	0.17996252228370935
1106	8	0.1769366448358909
1106	9	0.27981086325790017
1106	10-11	0.269265607075738
1106	12-13	0.19507066292848663
1106	14-15	0.34471508873233603
1106	16-17	0.4580062018997637
1106	18-19	0.47585741864784836
1106	20-21	0.3244467592534903
1106	22-23	0.6208171998937857
1106	24-25	0.3715310696055383
1106	26-27	0.2776908738016388
1106	28-29	0.4581556124503834
1106	30-31	0.4219354515783067
1106	32-33	0.32207880503558783
1106	34-35	0.24327565204804102
1106	36-37	0.24215319648482136
1106	38-39	0.37963802890151044
1106	40-41	0.2719877603942962
1106	42-43	0.20361403933964084
1106	44-45	0.24343867699663946
1106	46-47	0.23928130363897537
1106	48-49	0.28216958812556925
1106	50-51	0.34951734619545505
1106	52-53	0.22575612745568208
1106	54-55	0.24637945392345983
1106	56-57	0.1555539186362509
1106	58-59	0.2248263118400402
1106	60-61	0.22570995800733584
1106	62-63	0.13943569466679406
1106	64-65	0.21730590082042056
1106	66-67	0.33503421884380913
1106	68-69	0.21593364693026018
1106	70-71	0.22491183410374305
1106	72-73	0.1586936454885688
1106	74-75	0.16546927223213004
1106	76-77	0.19685741839135318
1106	78-79	0.2198604515130711
1106	80-81	0.2478312448109108
1106	82-83	0.25795327037305427
1106	84-85	0.25367685831605513
1106	86-87	0.18871035006822723
1106	88-89	0.3030209583029624
1106	90-91	0.21164912964703575
1106	92-93	0.31477545867850765
1106	94-95	0.30770848853808985
1106	96-97	0.27190978358743934
1106	98-99	0.2393259986048264
1106	100	0.20352195335758339
1107	1	0.28541097325585696
1107	2	0.3976786762158042
1107	3	0.2911728229664128
1107	4	0.468107400646268
1107	5	0.37806453367747395
1107	6	0.17580195800360343
1107	7	0.19178872886703857
1107	8	0.260882412618443
1107	9	0.2879863928131883
1107	10-11	0.3170912749940129
1107	12-13	0.20453187574342735
1107	14-15	0.3658081827293884
1107	16-17	0.5576352260446313
1107	18-19	0.5869666593941716
1107	20-21	0.3713085566784642
1107	22-23	0.675566380674006
1107	24-25	0.347203427242178
1107	26-27	0.3068081628376902
1107	28-29	0.4427983771752153
1107	30-31	0.4805332654959287
1107	32-33	0.3775797074315932
1107	34-35	0.2505485380517243
1107	36-37	0.23900727040340541
1107	38-39	0.2318780272908043
1107	40-41	0.08046262533195403
1107	42-43	0.25243542896348004
1107	44-45	0.22410317580048655
1107	46-47	0.19027408777159138
1107	48-49	0.13195531033282748
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2216	1	0.3630400516353376
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2216	4	0.38117468095696694
2216	5	0.11112419894102032
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2216	10-11	0.34668524275179635
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2216	80-81	0.041212801939721544
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2216	90-91	-0.007247727980939089
2216	92-93	-0.06886489412732644
2216	94-95	0.11848866292675808
2216	96-97	0.046489308876918045
2216	98-99	0.12195204527017722
2216	100	0.06718115784226697
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	17182.0
1	2886.0
2	482.0
3	405.0
4	384.0
5	254.0
6	177.0
7	286.0
8	502.0
9	890.0
10	1408.0
11	2682.0
12	4534.0
13	6272.0
14	8086.0
15	10250.0
16	11949.0
17	14455.0
18	16327.0
19	18736.0
20	21686.0
21	24596.0
22	28997.0
23	33663.0
24	38588.0
25	44947.0
26	52116.0
27	61431.0
28	73718.0
29	87959.0
30	107281.0
31	131851.0
32	164893.0
33	211852.0
34	295747.0
35	409051.0
36	638811.0
37	948562.0
38	592247.0
39	142700.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.413847562136205	20.522065210148895	11.83475815998546	24.229329067729438
2	15.98917276545774	19.465839961008275	39.90865827229792	24.636329001236067
3	19.15136017973846	26.372404177740734	27.99795760388635	26.478278038634457
4	12.639378268868212	16.29713726965882	39.65177526150946	31.41170919996351
5	13.488572244848134	38.741204966232914	34.28488036711689	13.485342421802066
6	30.18008984532974	37.979872479129476	17.83804744894104	14.00199022659975
7	27.11850832602563	31.12776237021405	23.77022688330731	17.983502420453007
8	26.603089228481895	34.56607305226364	19.349220087922806	19.48161763133166
9	26.27109499044486	14.322702250449234	21.351885832723262	38.05431692638264
10-11	26.763136809382143	24.819018936137414	26.558148750774528	21.859695503705915
12-13	26.684951077809576	22.530522882653305	26.693639639810968	24.09088639972615
14-15	24.047893803445902	23.68704649688698	24.07430364254913	28.190756057117994
16-17	23.19189842122623	26.2405215629058	25.478514353974568	25.0890656618934
18-19	22.320683255172938	27.468334194078274	26.96574631340664	23.245236237342144
20-21	24.461769540981084	26.7524960607282	25.700240700590737	23.08549369769998
22-23	25.243868649078184	25.97384682506188	25.60568536536217	23.176599160497773
24-25	24.616798799000815	25.83781305189263	26.317532878969097	23.22785527013746
26-27	24.483742229514867	26.274863413433064	24.68648308722242	24.554911269829653
28-29	23.264594160481934	26.77244770050497	26.037683111811045	23.92527502720206
30-31	24.093780750417135	26.53130541017194	26.153458545466645	23.221455293944278
32-33	24.377495706959156	26.556205765991507	24.898270174023697	24.16802835302564
34-35	24.43734845243189	26.559299671944085	25.913362335377737	23.08998954024628
36-37	24.05038581828332	25.80201081061546	25.07539469873743	25.07220867236379
38-39	24.343166865909627	26.029502856225196	25.88858457301998	23.7387457048452
40-41	23.808436953584238	24.786087646202716	27.008049136213604	24.397426263999446
42-43	23.772978349795377	25.182835414605247	26.209749700564068	24.834436535035312
44-45	24.257272608390693	25.922560243470404	26.039147853584886	23.781019294554014
46-47	23.975433636473976	25.943662417213286	25.29167563717133	24.789228309141404
48-49	24.91628590495308	25.60979218700139	25.648358609493283	23.825563298552243
50-51	24.15920922911535	25.189739415423112	26.10978390924174	24.5412674462198
52-53	23.907074029775742	25.68066556898544	25.757684709549295	24.65457569168953
54-55	23.57077004419027	25.63207916781176	25.706014897455187	25.091135890542787
56-57	25.09937079918214	25.045458656720022	25.187453446401452	24.667717097696386
58-59	23.27325954905735	25.779741871912986	26.491249235582142	24.45574934344752
60-61	23.505341912467774	26.341711962577435	25.634428988653923	24.518517136300865
62-63	24.18602467715521	25.599149538996897	24.90455488658539	25.310270897262505
64-65	23.129026433957538	25.85454209622922	25.697552698362248	25.318878771450986
66-67	24.220006356476627	25.519839267193806	25.5335316027901	24.726622773539468
68-69	23.320829294307607	25.400330159889545	26.16322157684991	25.11561896895294
70-71	23.15122867099469	25.24058388170602	25.925244446916786	25.6829430003825
72-73	23.838511038501625	25.20316814886382	26.567765825529293	24.390554987105265
74-75	24.199073074185087	25.457059105516944	25.994788285850507	24.349079534447462
76-77	23.78209243801149	25.512158573826305	25.213747540081897	25.49200144808031
78-79	23.997786345484656	25.605776901192783	25.01544921612615	25.380987537196408
80-81	23.331067139556332	26.206629329935627	26.054243645941295	24.408059884566747
82-83	24.444751091956338	24.88016678772112	27.341928258982602	23.333153861339937
84-85	24.3063286787848	25.372959400421692	26.014121737162434	24.30659018363108
86-87	22.66316647657891	26.300714146559212	26.529010221817273	24.507109155044606
88-89	24.25633287233624	24.43383010163175	26.339319282345997	24.970517743686017
90-91	23.63950274096817	25.042810071501126	25.878464101576593	25.43922308595411
92-93	24.04245939763921	24.925520104192962	26.335940548045812	24.69607995012202
94-95	22.930882774658347	25.06117570921208	27.178092527580322	24.829848988549255
96-97	23.302605745065325	24.91699343764665	26.55576494291439	25.22463587437364
98-99	22.570939446033645	25.55933237371687	26.403492866881486	25.466235313368003
100	23.591287280560742	24.471876899599778	25.48563421503846	26.451201604801017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14222.0
1	8344.5
2	2245.5
3	1541.0
4	746.0
5	365.5
6	271.0
7	240.0
8	224.5
9	216.5
10	205.5
11	183.5
12	166.0
13	153.5
14	162.0
15	183.5
16	195.5
17	204.0
18	230.5
19	258.5
20	271.5
21	331.5
22	447.5
23	595.0
24	819.0
25	1078.0
26	1487.0
27	2073.0
28	3204.5
29	5887.5
30	9870.0
31	14432.0
32	21774.5
33	31433.5
34	41238.0
35	51259.5
36	63280.0
37	84565.0
38	101095.0
39	106591.5
40	103839.5
41	97327.5
42	98603.5
43	109347.5
44	128120.5
45	161130.5
46	194791.0
47	219658.5
48	236386.0
49	234787.5
50	224432.5
51	233744.0
52	241040.5
53	230276.5
54	215851.5
55	183443.5
56	159819.5
57	131397.5
58	100206.5
59	97039.0
60	80773.0
61	54025.5
62	39697.0
63	33721.5
64	24867.0
65	10868.5
66	5158.0
67	3328.0
68	2402.0
69	1793.0
70	1419.0
71	1100.0
72	822.0
73	632.0
74	516.0
75	420.0
76	331.0
77	235.5
78	150.5
79	97.5
80	61.0
81	44.5
82	37.0
83	23.0
84	16.0
85	17.5
86	16.5
87	12.0
88	12.0
89	10.0
90	8.5
91	8.0
92	6.0
93	6.5
94	5.0
95	3.0
96	4.0
97	2.5
98	1.5
99	2.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9836387326225086
2	0.44375710883943537
3	0.47549120454776544
4	0.46068826571511673
5	0.428788641936773
6	0.48059892785104363
7	0.4965842100409327
8	0.4991380716925718
9	0.5138464230566414
10-11	0.5274079013641878
12-13	0.5251969192861484
14-15	0.5231751121452675
16-17	0.527384254497043
18-19	0.5264383798112506
20-21	0.5252323895868656
22-23	0.4890645062888843
24-25	0.521638065780855
26-27	0.5037255639186642
28-29	0.5081238812075982
30-31	0.5114817363421607
32-33	0.5152770585189022
34-35	0.5270886686577329
36-37	0.5444454691420207
38-39	0.5332723244160997
40-41	0.5388293381951292
42-43	0.5355542470955735
44-45	0.5448238190163377
46-47	0.5433222429526424
48-49	0.5169796329533282
50-51	0.5311795766737843
52-53	0.5236244026210188
54-55	0.5328585042410656
56-57	0.5213070096408278
58-59	0.5263201454755266
60-61	0.5206212504936284
62-63	0.5145558290709855
64-65	0.5258235612654858
66-67	0.5239436353274737
68-69	0.5450366408206408
70-71	0.5285784212878557
72-73	0.5325747418353279
74-75	0.5297962349458132
76-77	0.5188359120241954
78-79	0.5480161460808864
80-81	0.5233051699145639
82-83	0.5227258216695161
84-85	0.5312741641423636
86-87	0.8326771327700649
88-89	1.0946016566995123
90-91	1.0848946177365693
92-93	1.0849655583380036
94-95	1.0892101709904962
96-97	1.082719105959247
98-99	1.0788055494467814
100	1.0745845836614336
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4228890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.11882185073493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.88320125408869	15.392073431963595
2	12.404178803639624	5.23923284717455
3	5.15841967499252	3.2681923844247898
4	2.744786914822369	2.3186666348944787
5	1.573979167914769	1.662029282198
6	1.004675547929048	1.2730538348701839
7	0.6804047132642537	1.0058542148079708
8	0.4798657609440181	0.8107359614115258
9	0.35314607054615277	0.6712226056036142
>10	1.9591448421432023	7.780975822369536
>50	0.2676275009080952	4.036328380197246
>100	0.35197584004163834	16.89349402720706
>500	0.07361749323911015	10.772040050201436
>1k	0.06138531829541653	22.79704744905364
>5k	0.0031422100772790914	4.446910711521868
>10k+	4.4888715389701305E-4	1.63214236210056
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	24862	0.5879084109541748	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	20313	0.48033881231245074	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12876	0.3044770613565262	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	10816	0.25576451503822517	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	9286	0.2195848083066715	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	8815	0.20844713388146774	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	8745	0.20679185318133128	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	8612	0.20364681985107205	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	8234	0.19470830407033524	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	8025	0.1897661088370707	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7477	0.17680762564171684	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7166	0.1694534499596821	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7066	0.16708876324520147	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6980	0.16505513267074812	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6967	0.16474772339786564	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	6686	0.15810295373017505	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	6598	0.15602202942143212	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	6516	0.15408298631555797	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6493	0.15353910837122745	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6408	0.1515291246639189	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6383	0.15093795298529875	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	6264	0.14812397579506678	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	5988	0.14159744046310024	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	5833	0.13793217605565525	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5746	0.13587489861405713	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5735	0.13561478307546423	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	5478	0.12953753821924902	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5393	0.12752755451194048	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5361	0.12677085476330668	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	5228	0.12362582143304744	No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG	5076	0.12003149762703688	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5075	0.12000785075989208	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	4985	0.11787963271685951	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	4879	0.11537306479951004	No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC	4717	0.11154227232205141	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	4657	0.11012346029336303	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	4486	0.10607984601160114	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4373	0.10340775002423803	No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA	4366	0.1032422219542244	No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC	4348	0.10281657834561787	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.1823433572403161E-5	0.0
18-19	0.0	0.0	0.0	2.3646867144806322E-5	0.0
20-21	0.0	0.0	0.0	3.5470300717209486E-5	0.0
22-23	0.0	0.0	0.0	9.458746857922529E-5	0.0
24-25	0.0	0.0	0.0	1.6552807001364423E-4	0.0
26-27	0.0	0.0	0.0	3.4287957359969165E-4	0.0
28-29	0.0	0.0	0.0	0.0012296370915299286	0.0
30-31	0.0	0.0	0.0	0.0061718323247944495	0.0
32-33	0.0	0.0	0.0	0.018716495345114204	0.0
34-35	0.0	0.0	0.0	0.038272454473869025	0.0
36-37	0.0	0.0	0.0	0.07339987561747882	0.0
38-39	0.0	0.0	0.0	0.13073170501006173	0.0
40-41	0.0	0.0	0.0	0.19584335369328595	0.0
42-43	0.0	0.0	0.0	0.2682973546249725	0.0
44-45	0.0	0.0	0.0	0.35608634890006596	0.0
46-47	0.0	0.0	0.0	0.4699696610694532	0.0
48-49	0.0	0.0	0.0	0.6066958469007233	0.0
50-51	0.0	0.0	0.0	0.755812991115872	0.0
52-53	0.0	0.0	0.0	0.9040079075123733	0.0
54-55	0.0	0.0	0.0	1.071049377023285	0.0
56-57	0.0	0.0	0.0	1.278976279827567	0.0
58-59	0.0	0.0	0.0	1.5327189877249112	0.0
60-61	0.0	0.0	0.0	1.7936858135349938	0.0
62-63	0.0	0.0	0.0	2.072565141207267	0.0
64-65	0.0	0.0	0.0	2.3698890252524896	0.0
66-67	0.0	0.0	0.0	2.708760454871136	0.0
68-69	0.0	0.0	0.0	3.0855614593900524	0.0
70-71	0.0	0.0	0.0	3.5118317099759038	0.0
72-73	0.0	0.0	0.0	3.9586156178098744	0.0
74-75	0.0	0.0	0.0	4.39991345246625	0.0
76-77	0.0	0.0	0.0	4.906405699840857	0.0
78-79	0.0	0.0	0.0	5.4492904757513205	0.0
80-81	0.0	0.0	0.0	6.033273506759457	0.0
82-83	0.0	0.0	0.0	6.640536878471655	0.0
84-85	0.0	0.0	0.0	7.228386172257968	0.0
86-87	0.0	0.0	0.0	7.861802979032323	0.0
88	0.0	0.0	0.0	8.372953659234456	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5635	0.0	45.83048	94
GTACTAG	6500	0.0	37.811775	1
TAGGCAT	8205	0.0	33.779068	5
GCATCGT	7015	0.0	33.394367	8
CTGTGCG	8735	0.0	31.390394	9
TAGTACT	8775	0.0	30.623451	4
CTAGGCA	9175	0.0	30.61966	4
CAACGCA	7090	0.0	30.610468	5
TATTCTC	1200	0.0	30.142805	5
ACTGTGC	9235	0.0	29.687683	8
ACGTTAA	700	0.0	28.838373	8
TCTAACG	3570	0.0	28.560379	2
TACTGTG	9960	0.0	28.51617	7
GTACTGT	9585	0.0	28.308067	6
TCAAGAC	810	0.0	27.262432	3
CTAACGC	3655	0.0	27.252115	3
TAACGCC	3660	0.0	27.211971	4
CGCCTAC	3710	0.0	26.952614	7
GTACATG	17655	0.0	26.880228	1
TACCCCG	350	0.0	26.843315	5
>>END_MODULE