##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4228890
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.769924495553205	31.0	31.0	34.0	30.0	34.0
2	31.77169020712291	31.0	31.0	34.0	30.0	34.0
3	31.958610651967774	31.0	31.0	34.0	30.0	34.0
4	35.69809737306953	37.0	35.0	37.0	33.0	37.0
5	35.61271255577705	37.0	35.0	37.0	33.0	37.0
6	35.64441780230746	37.0	35.0	37.0	33.0	37.0
7	35.57126716466969	37.0	35.0	37.0	33.0	37.0
8	35.552957631908136	37.0	35.0	37.0	33.0	37.0
9	37.152221977871264	39.0	37.0	39.0	33.0	39.0
10-11	37.099783394696956	39.0	37.0	39.0	33.0	39.0
12-13	37.02604678769133	39.0	37.0	39.0	33.0	39.0
14-15	38.23122036751961	40.0	37.0	41.0	33.0	41.0
16-17	38.2136281624729	40.0	37.0	41.0	33.0	41.0
18-19	38.235694354783405	40.0	37.0	41.0	33.0	41.0
20-21	38.24063312122094	40.0	37.0	41.0	33.0	41.0
22-23	38.19496581845354	40.0	37.0	41.0	33.0	41.0
24-25	38.11968696277274	40.0	37.0	41.0	33.0	41.0
26-27	37.98375306522516	40.0	37.0	41.0	33.0	41.0
28-29	37.91540451513281	40.0	37.0	41.0	33.0	41.0
30-31	37.81928804012401	40.0	37.0	41.0	32.0	41.0
32-33	37.74416478082901	40.0	37.0	41.0	32.0	41.0
34-35	37.66598634629891	40.0	36.5	41.0	32.0	41.0
36-37	37.59332779996642	40.0	36.0	41.0	32.0	41.0
38-39	37.47638753904689	40.0	36.0	41.0	31.0	41.0
40-41	37.35469792309566	39.5	36.0	41.0	31.0	41.0
42-43	37.235230994421705	39.0	36.0	41.0	31.0	41.0
44-45	37.04033410658589	39.0	35.0	41.0	31.0	41.0
46-47	36.84641005086441	39.0	35.0	41.0	31.0	41.0
48-49	36.60282355417142	39.0	35.0	41.0	30.0	41.0
50-51	36.36999626379499	38.0	35.0	40.5	30.0	41.0
52-53	36.13672417584756	38.0	35.0	40.0	30.0	41.0
54-55	35.87682961722816	37.5	34.5	40.0	29.0	41.0
56-57	35.57025271406918	37.0	34.0	40.0	29.0	41.0
58-59	35.236290019366784	36.5	34.0	39.5	28.0	41.0
60-61	34.95224101359931	36.0	34.0	39.0	28.0	41.0
62-63	34.631546457817535	35.0	33.5	39.0	28.0	41.0
64-65	34.326196354126026	35.0	33.0	38.0	28.0	40.5
66-67	34.08689478799401	35.0	33.0	37.0	28.0	40.0
68-69	33.87278174651031	35.0	33.0	37.0	27.0	40.0
70-71	33.70195216711714	35.0	33.0	36.5	27.0	39.5
72-73	33.51693363979673	35.0	33.0	36.0	27.0	39.0
74-75	33.365906538122296	35.0	33.0	36.0	27.0	39.0
76-77	33.26027964785085	35.0	33.0	35.0	27.0	38.0
78-79	33.18525334071116	35.0	33.0	35.0	27.0	37.0
80-81	33.08383878511855	35.0	33.0	35.0	27.5	37.0
82-83	32.93158914041273	35.0	33.0	35.0	27.0	36.0
84-85	32.84450482277855	35.0	33.0	35.0	27.0	36.0
86-87	32.75621368727964	35.0	33.0	35.0	27.0	36.0
88-89	32.69136132649466	35.0	33.0	35.0	27.0	36.0
90-91	32.61596376354079	35.0	33.0	35.0	27.0	35.0
92-93	32.55494597873201	35.0	33.0	35.0	27.0	35.0
94-95	32.534872271447114	35.0	33.0	35.0	27.0	35.0
96-97	32.48977970578568	35.0	33.0	35.0	27.0	35.0
98-99	32.45723535017463	35.0	33.0	35.0	27.0	35.0
100	31.91413846186588	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5205645581233362
1101	2	0.6253714395609116
1101	3	0.5128532078781518
1101	4	0.3610733369415442
1101	5	0.4014316895200096
1101	6	0.41492635734005034
1101	7	0.45174392353223425
1101	8	0.45684141545643087
1101	9	0.5302539259718486
1101	10-11	0.5942771842998624
1101	12-13	0.5607706931586023
1101	14-15	0.7563773994047551
1101	16-17	0.6770430760371795
1101	18-19	0.6826662123607932
1101	20-21	0.7033318243823601
1101	22-23	0.7164193226626168
1101	24-25	0.7194026152957491
1101	26-27	0.7797436135776721
1101	28-29	0.7231806655238842
1101	30-31	0.8352631518916596
1101	32-33	0.8445444268463689
1101	34-35	0.8039343140337394
1101	36-37	0.8393975492487087
1101	38-39	0.8512916102886976
1101	40-41	0.8476723885811452
1101	42-43	0.8477091062414672
1101	44-45	0.8808593633016244
1101	46-47	0.8686966158441223
1101	48-49	0.8752657323233208
1101	50-51	0.8140751053906499
1101	52-53	0.8113012326444675
1101	54-55	0.7523224611203929
1101	56-57	0.7866493024229584
1101	58-59	0.7494970439162643
1101	60-61	0.7817768754099248
1101	62-63	0.7768783419403107
1101	64-65	0.796864103171032
1101	66-67	0.7280085748973164
1101	68-69	0.7391533654742872
1101	70-71	0.703998158362829
1101	72-73	0.6878397230218241
1101	74-75	0.6292828258828038
1101	76-77	0.6807803035367854
1101	78-79	0.6799153310179236
1101	80-81	0.616583189131525
1101	82-83	0.5742798258124822
1101	84-85	0.5851735112290939
1101	86-87	0.6254358931389774
1101	88-89	0.6297144936588737
1101	90-91	0.600103313213431
1101	92-93	0.6427064315111863
1101	94-95	0.6131338263792614
1101	96-97	0.6584907352282059
1101	98-99	0.6654496198511453
1101	100	0.7346833164295923
1102	1	0.5149344992341547
1102	2	0.5655507528844161
1102	3	0.47656578598270727
1102	4	0.3984738991846797
1102	5	0.3770060055822668
1102	6	0.41132331033882963
1102	7	0.4003282807401689
1102	8	0.39046011316326457
1102	9	0.5126815635066535
1102	10-11	0.49984164656726904
1102	12-13	0.5442919255360721
1102	14-15	0.6860071455658527
1102	16-17	0.7046086738507569
1102	18-19	0.7024350605441825
1102	20-21	0.6897879811926089
1102	22-23	0.6905193832536796
1102	24-25	0.706855831222569
1102	26-27	0.7375193637079391
1102	28-29	0.7585086891539206
1102	30-31	0.7768335331100502
1102	32-33	0.7578450953503122
1102	34-35	0.780610329172454
1102	36-37	0.7355789773005128
1102	38-39	0.7951442867595802
1102	40-41	0.766572844277448
1102	42-43	0.8233284723092282
1102	44-45	0.7916672648342313
1102	46-47	0.7523133828364408
1102	48-49	0.7706522303837886
1102	50-51	0.7539543237039368
1102	52-53	0.6950738869574309
1102	54-55	0.771959734684522
1102	56-57	0.787188233970312
1102	58-59	0.7515059404580882
1102	60-61	0.7379975660908684
1102	62-63	0.7042746566114957
1102	64-65	0.6835802628701941
1102	66-67	0.703196748055241
1102	68-69	0.6267157025255585
1102	70-71	0.641422985635387
1102	72-73	0.6613164726901459
1102	74-75	0.6627034942532646
1102	76-77	0.644856857082651
1102	78-79	0.6147422746396316
1102	80-81	0.6007346448664421
1102	82-83	0.64323160037749
1102	84-85	0.6047745541009704
1102	86-87	0.6345581617142457
1102	88-89	0.5983062972275022
1102	90-91	0.6325083304015564
1102	92-93	0.580538854071655
1102	94-95	0.628674171400327
1102	96-97	0.6397773443166841
1102	98-99	0.6224280563919038
1102	100	0.6338361379662594
1103	1	0.3365802611897415
1103	2	-0.049957498044118864
1103	3	0.24880303854718733
1103	4	0.2511449153223708
1103	5	0.3016772269754284
1103	6	0.33918166088972157
1103	7	0.373038593368868
1103	8	0.3909960327155346
1103	9	0.41753789347055204
1103	10-11	0.4991470781919034
1103	12-13	0.4680554604739342
1103	14-15	0.6429391887278513
1103	16-17	0.6241928701312673
1103	18-19	0.6100109013544923
1103	20-21	0.6147714516094922
1103	22-23	0.6003886022059177
1103	24-25	0.5694754579700501
1103	26-27	0.6327666236146428
1103	28-29	0.6480745934889356
1103	30-31	0.6607001220196622
1103	32-33	0.6683101655556456
1103	34-35	0.6087161760255952
1103	36-37	0.6417324618380178
1103	38-39	0.6421242225199961
1103	40-41	0.7467909344228829
1103	42-43	0.7384651839365688
1103	44-45	0.7163756267083201
1103	46-47	0.7168270913465662
1103	48-49	0.6996580148843705
1103	50-51	0.7174017072811978
1103	52-53	0.6860579472768791
1103	54-55	0.6433547983938936
1103	56-57	0.6853047356928954
1103	58-59	0.6514499607527213
1103	60-61	0.6256871726428201
1103	62-63	0.6795406378035196
1103	64-65	0.6628128163879268
1103	66-67	0.7016427448131566
1103	68-69	0.6384451216039508
1103	70-71	0.6021328985125081
1103	72-73	0.5239574422822244
1103	74-75	0.5402257544884463
1103	76-77	0.5283800465540409
1103	78-79	0.5447932584847592
1103	80-81	0.5668092570924088
1103	82-83	0.533567305839199
1103	84-85	0.5102767870301363
1103	86-87	0.5332377710625948
1103	88-89	0.5294837653081572
1103	90-91	0.5169929617429716
1103	92-93	0.5415342106838992
1103	94-95	0.5116780976354036
1103	96-97	0.5413283783893945
1103	98-99	0.5587827212932055
1103	100	0.573473173046839
1104	1	0.424840956206733
1104	2	0.4847800037145298
1104	3	0.42362839789078777
1104	4	0.3064455137001403
1104	5	0.3161160088223838
1104	6	0.3117473981837975
1104	7	0.3272365914239259
1104	8	0.34578689424862574
1104	9	0.41444061469536564
1104	10-11	0.42260260028951535
1104	12-13	0.39817157492998234
1104	14-15	0.5516363063002458
1104	16-17	0.5070506431929758
1104	18-19	0.5353198948522717
1104	20-21	0.5166274482452593
1104	22-23	0.5330686038344439
1104	24-25	0.5741604399816111
1104	26-27	0.6004423194587716
1104	28-29	0.5864167402381071
1104	30-31	0.5944386096508936
1104	32-33	0.6172238246581188
1104	34-35	0.5790555831518063
1104	36-37	0.5986049420780546
1104	38-39	0.5788891792585886
1104	40-41	0.6045448249606125
1104	42-43	0.6118063747036899
1104	44-45	0.6310854848673841
1104	46-47	0.6284834758379034
1104	48-49	0.606425543240448
1104	50-51	0.5784668001248363
1104	52-53	0.5787246630739844
1104	54-55	0.5939029548946024
1104	56-57	0.5517747603732062
1104	58-59	0.541957497034943
1104	60-61	0.5698551410083255
1104	62-63	0.5986479103274576
1104	64-65	0.5326934036649646
1104	66-67	0.4880397128812959
1104	68-69	0.4979139047959791
1104	70-71	0.5563765054147538
1104	72-73	0.5520081716888043
1104	74-75	0.47772246266345775
1104	76-77	0.41925425735349364
1104	78-79	0.3698467909028622
1104	80-81	0.3716806427201007
1104	82-83	0.3722715404531698
1104	84-85	0.39261415541511724
1104	86-87	0.42288208905056734
1104	88-89	0.4787351360215055
1104	90-91	0.4545390923862911
1104	92-93	0.5275847576550845
1104	94-95	0.4780983906348766
1104	96-97	0.5051489670991174
1104	98-99	0.4599586049839459
1104	100	0.5741466379226203
1105	1	0.17537170914775402
1105	2	-0.37991250284633793
1105	3	0.09116600948456721
1105	4	0.14598448808309428
1105	5	0.15377400526536178
1105	6	0.27865433437987264
1105	7	0.3165140305313514
1105	8	0.287749547042111
1105	9	0.36727834566165996
1105	10-11	0.3586760202993915
1105	12-13	0.3800689865041278
1105	14-15	0.45769290727177747
1105	16-17	0.45947157238703085
1105	18-19	0.48739103121392446
1105	20-21	0.5082285662256965
1105	22-23	0.5453728634755848
1105	24-25	0.5469866704872288
1105	26-27	0.5142375793984755
1105	28-29	0.5379477454903991
1105	30-31	0.551953451565808
1105	32-33	0.5899804768627064
1105	34-35	0.5721009904443761
1105	36-37	0.5713189942741081
1105	38-39	0.6230094788049954
1105	40-41	0.5620791426333511
1105	42-43	0.5520535678050607
1105	44-45	0.5992366599133305
1105	46-47	0.57954151308234
1105	48-49	0.5450852308369605
1105	50-51	0.5556547781866072
1105	52-53	0.5273809128652189
1105	54-55	0.5185565407721597
1105	56-57	0.532678702749628
1105	58-59	0.5246634865755908
1105	60-61	0.49043459362133746
1105	62-63	0.4518941183357512
1105	64-65	0.49079339189728444
1105	66-67	0.4714173334370031
1105	68-69	0.41771586871290367
1105	70-71	0.4907710020486249
1105	72-73	0.42679544142907844
1105	74-75	0.4627653889808556
1105	76-77	0.41770020493132876
1105	78-79	0.39285218296425484
1105	80-81	0.40378866483099785
1105	82-83	0.4265812584452249
1105	84-85	0.44264917580813545
1105	86-87	0.4424906828443298
1105	88-89	0.4232778385475484
1105	90-91	0.4340375428045604
1105	92-93	0.4242528100638907
1105	94-95	0.409981953352208
1105	96-97	0.39382897834263986
1105	98-99	0.4080147033884245
1105	100	0.4447269898120503
1106	1	0.28783609736090554
1106	2	0.3085270398024953
1106	3	0.25712910665673405
1106	4	0.18340611789194838
1106	5	0.183095656827426
1106	6	0.19100071748385972
1106	7	0.19539014558561973
1106	8	0.19133322695775234
1106	9	0.225128849341516
1106	10-11	0.25643905827694624
1106	12-13	0.2777192049662176
1106	14-15	0.3476589856043617
1106	16-17	0.3611326706897131
1106	18-19	0.37934194860913095
1106	20-21	0.3659453766064331
1106	22-23	0.3713369997881628
1106	24-25	0.3671660900080269
1106	26-27	0.36368688551953454
1106	28-29	0.3280661266309295
1106	30-31	0.3438767427863354
1106	32-33	0.3576339259597887
1106	34-35	0.3503073249149651
1106	36-37	0.37907116373243355
1106	38-39	0.3166396996769194
1106	40-41	0.311971751312484
1106	42-43	0.300299240007611
1106	44-45	0.2798235457995446
1106	46-47	0.24503046706014686
1106	48-49	0.29354154124963117
1106	50-51	0.30064389753734133
1106	52-53	0.33199099665147
1106	54-55	0.2983883819753288
1106	56-57	0.3230860316412105
1106	58-59	0.3025671764540405
1106	60-61	0.2768249776504348
1106	62-63	0.1908853151586456
1106	64-65	0.19989147057922452
1106	66-67	0.29945953830697647
1106	68-69	0.31346666383727495
1106	70-71	0.30959639532486705
1106	72-73	0.2923817693332751
1106	74-75	0.2648290935447477
1106	76-77	0.25604273755508444
1106	78-79	0.2307797467905246
1106	80-81	0.28172731956062336
1106	82-83	0.2787053200852583
1106	84-85	0.19141109256530342
1106	86-87	0.22634560469045795
1106	88-89	0.24011570393479076
1106	90-91	0.17280339373926523
1106	92-93	0.22522017094643587
1106	94-95	0.27339060482290023
1106	96-97	0.25142101100642833
1106	98-99	0.3178574757827093
1106	100	0.33590246383394273
1107	1	0.11140018530062079
1107	2	-0.2960944555739644
1107	3	0.03709895197536284
1107	4	0.07676479356805288
1107	5	0.1165419711965825
1107	6	0.18173696568170783
1107	7	0.1880127116909165
1107	8	0.17247664917081806
1107	9	0.17220385184999998
1107	10-11	0.23232026414734008
1107	12-13	0.23642815155916708
1107	14-15	0.28305185462953375
1107	16-17	0.2879308284023949
1107	18-19	0.29408310222373046
1107	20-21	0.25829174484209005
1107	22-23	0.23713670286225152
1107	24-25	0.2294426859352896
1107	26-27	0.33056203734915357
1107	28-29	0.2694943052828904
1107	30-31	0.30588526042760833
1107	32-33	0.32835399770258533
1107	34-35	0.355157455884509
1107	36-37	0.3566961822160408
1107	38-39	0.4007232693363463
1107	40-41	0.39753764008342785
1107	42-43	0.38753459439112703
1107	44-45	0.33264968901051617
1107	46-47	0.37160058329561707
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2216	100	-1.3196387807740386
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	45.0
14	139.0
15	470.0
16	1152.0
17	2236.0
18	4241.0
19	6520.0
20	9592.0
21	13953.0
22	19524.0
23	24220.0
24	28293.0
25	34527.0
26	43283.0
27	55593.0
28	70654.0
29	88051.0
30	109036.0
31	134021.0
32	165898.0
33	209537.0
34	287434.0
35	396447.0
36	626737.0
37	1043945.0
38	677353.0
39	175836.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.48664613195695	21.43524755172779	13.125525035191549	24.95258128112371
2	15.499881525049295	20.11950599019756	39.04234403517642	25.338268449576727
3	18.750579359479076	26.049279134924074	28.95090058475107	26.24924092084578
4	13.069631091549697	16.990371406189094	38.22618916965323	31.71380833260798
5	13.185198392813774	38.85219699311169	34.59517773189616	13.367426882178378
6	30.161011518388985	38.404758695544224	17.46862651901563	13.965603267051167
7	26.435457924172308	31.534258385855157	24.016801623693475	18.013482066279064
8	26.415536937588826	34.9350065856525	19.152448987795854	19.497007488962826
9	26.00195323122616	14.19372459439711	21.391452603401838	38.41286957097489
10-11	26.72852687111748	25.02175511777322	26.510644637245235	21.739073373864063
12-13	26.6174693595719	22.6546209525431	27.020423799152972	23.70748588873203
14-15	24.007098962032803	23.734484914350944	24.365712661364636	27.892703462251617
16-17	22.97299310532703	26.335482262935905	25.68746908085846	25.0040555508786
18-19	22.23591769944359	27.16588750239424	27.595811193953967	23.002383604208195
20-21	24.146161372228512	26.425460596906397	26.67000481805203	22.758373212813062
22-23	24.859069483787227	25.636478131088374	26.43125553784391	23.07319684728048
24-25	24.277871971131905	25.493415529843528	27.176516769175834	23.05219572984873
26-27	24.13551945803189	25.95179082138347	25.692206938308892	24.22048278227575
28-29	23.033290059566458	26.495747110944006	26.865229410081604	23.60573341940793
30-31	23.929872850795363	26.188917186306572	26.844325579525595	23.03688438337247
32-33	24.281998349448674	26.18908271437659	25.852469560570267	23.676449375604474
34-35	24.191187694208182	26.2603926982394	26.68951437505594	22.85890523249648
36-37	23.928643213703833	25.4911572540312	25.89995246979704	24.680247062467927
38-39	24.13428819382864	25.606412084494984	26.799609353754768	23.459690367921606
40-41	23.499440631109046	24.542058841681932	27.874270520774687	24.08423000643434
42-43	23.699075802716525	24.8837570607107	26.883362628075325	24.533804508497443
44-45	24.167429278132087	25.511505383209304	26.679944382568475	23.641120956090134
46-47	23.99338833594631	25.684387628904986	25.724291717211845	24.59793231793686
48-49	25.079001666285755	25.386625470442258	26.060493988164442	23.47387887510755
50-51	24.07130476318845	25.0909576744725	26.374284977854707	24.463452584484344
52-53	23.703749683723153	25.46701380267635	26.07195978140836	24.757276732192135
54-55	23.56463184090602	25.561571213502233	25.833594139713163	25.040202805878593
56-57	25.08014520132602	24.943028744915622	25.408813240930773	24.568012812827583
58-59	23.10090468355466	25.7348987273705	26.767157371617365	24.397039217457476
60-61	23.554675681175176	26.23664645527848	25.810092089253285	24.398585774293068
62-63	24.252534352986245	25.39571849823476	25.048464254213282	25.303282894565715
64-65	23.13808115131867	25.73507468862988	25.83654339554824	25.290300764503215
66-67	24.07510008536519	25.40989479508807	25.80528223718281	24.70972288236393
68-69	23.35961487165226	25.092496164418286	26.419626354750164	25.12826260917929
70-71	23.040719419407786	25.016514519475663	26.244246521132375	25.698519539984172
72-73	23.79068739078103	24.879590152498647	26.730536854824788	24.59918560189553
74-75	24.035433482825187	25.34066040866834	26.24067380464551	24.38323230386096
76-77	23.65563338623686	25.359747340034026	25.28671185793777	25.69790741579134
78-79	23.75357394444049	25.59443170529439	25.20697604632537	25.445018303939754
80-81	23.1352055285453	26.259987156941534	26.13389769325096	24.4709096212622
82-83	24.292508825165246	24.799624764946028	27.404325466040614	23.50354094384811
84-85	24.288794803163515	25.361643238580072	25.93463941620132	24.41492254205509
86-87	22.54043847381853	26.23259939835218	26.488147779140075	24.738814348689214
88-89	23.96841950924192	24.542456533666307	26.253677374803715	25.235446582288063
90-91	23.31074761548123	24.999320110929517	25.967411202348938	25.722521071240312
92-93	23.869680017689426	25.103694795927172	26.009651142304097	25.016974044079305
94-95	22.678527786819547	25.037217736676844	26.96669918550767	25.31755529099594
96-97	23.08652780402049	24.999745783975026	26.28609069434555	25.62763571765893
98-99	22.272814499349874	25.663708338767304	26.263027790530945	25.800449371351874
100	23.321702460612382	24.60702316005518	25.35005629254039	26.721218086792042
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	54.0
1	38.5
2	35.5
3	36.0
4	17.5
5	18.0
6	52.0
7	128.0
8	220.0
9	250.5
10	190.5
11	131.5
12	117.0
13	147.0
14	197.0
15	267.0
16	376.5
17	505.5
18	681.0
19	903.0
20	1151.5
21	1501.5
22	1831.0
23	2114.5
24	2411.0
25	2723.5
26	3245.5
27	3795.0
28	4944.0
29	7951.0
30	12550.5
31	17355.0
32	25332.0
33	35086.0
34	44569.0
35	53925.5
36	63708.0
37	83886.0
38	98509.5
39	103068.5
40	101033.5
41	95089.0
42	96411.5
43	106074.0
44	124611.5
45	159708.5
46	195332.5
47	221520.5
48	240926.5
49	237561.5
50	224390.0
51	230754.5
52	236283.0
53	228161.0
54	219163.0
55	186966.0
56	161169.0
57	132184.5
58	99774.5
59	99641.0
60	83478.0
61	54191.0
62	38819.0
63	32382.5
64	23538.5
65	9650.5
66	4304.5
67	2930.5
68	2150.5
69	1583.5
70	1282.5
71	1002.0
72	691.0
73	561.5
74	468.5
75	365.5
76	283.0
77	203.0
78	123.0
79	58.5
80	30.5
81	19.5
82	14.0
83	4.0
84	0.0
85	0.0
86	0.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014235414021173403
2	0.0034997363374313357
3	0.0019390431058741184
4	0.025207560376363538
5	0.0031450333302592407
6	0.0
7	3.074092728824822E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009092220417178032
16-17	0.0027903303230871457
18-19	0.0
20-21	5.91171678620158E-5
22-23	7.803466157786087E-4
24-25	0.0
26-27	1.537046364412411E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.611139093237233E-4
36-37	0.0
38-39	0.0
40-41	4.0199674146170747E-4
42-43	7.330528814889959E-4
44-45	0.0
46-47	0.0
48-49	5.557013779029485E-4
50-51	0.0
52-53	0.0
54-55	4.3746704217891697E-4
56-57	7.094060143441897E-5
58-59	1.8917493715845055E-4
60-61	8.276403500682212E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0015606932315572173
70-71	8.394637836406243E-4
72-73	0.0
74-75	2.0099837073085373E-4
76-77	0.0029203880923835807
78-79	4.492904757513201E-4
80-81	0.003464266036714126
82-83	0.0017498681687156679
84-85	0.00568707154832592
86-87	0.007957170794227328
88-89	0.007803466157786086
90-91	0.0061245385905048366
92-93	0.008867575179302369
94-95	0.001406988595115976
96-97	0.0048003140303956825
98-99	0.0022109820780393907
100	0.08609824327423982
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4228890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.474701010157697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.53819574019464	10.141839838247027
2	15.513421077219942	4.801311056293143
3	6.6325037690301345	3.079080383234561
4	3.406087738123531	2.1083275744730376
5	1.9986152077728279	1.5463986387319357
6	1.2811297804883102	1.1895060184959336
7	0.8586870229452634	0.9301547458966262
8	0.6416781130050215	0.7943821554811914
9	0.45725425876564885	0.6368285646017726
>10	2.5831678416654342	7.590558081369404
>50	0.3689906144664566	4.073330197965192
>100	0.5064062523434193	17.56151482025432
>500	0.11227785658920729	11.97988896556546
>1k	0.09593264481363561	26.66715222836981
>5k	0.005193805610929316	5.522660614620067
>10k+	4.582769656702338E-4	1.377066116400545
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	27362	0.6470255788161905	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	19844	0.4692484316215367	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	11048	0.2612505882158202	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	9678	0.22885438022743557	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	9373	0.22164208574826966	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	8737	0.2066026782441728	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	8484	0.20062002085653682	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	8340	0.19721487198768473	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	8280	0.19579605995899635	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	8259	0.1952994757489554	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	8137	0.19241455795728904	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7994	0.18903305595558173	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	7663	0.18120594293065084	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7609	0.17992901210483128	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	7551	0.1785574938104325	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	7081	0.16744346625237355	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7068	0.16713605697949108	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7003	0.16559901061507867	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6628	0.1567314354357763	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	6627	0.1567077885686315	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	6554	0.15498156726706064	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	6465	0.15287699609117286	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	6212	0.14689433870353688	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	6210	0.14684704496924725	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	6209	0.14682339810210243	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	6102	0.14429318331760815	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5961	0.14095897505019048	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	5914	0.13984757229438458	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	5827	0.13779029485278643	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5724	0.1353546675368714	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	5668	0.13403044297676223	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	5566	0.13161846252799198	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	5494	0.1299158880935659	No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG	5433	0.12847342919773275	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	5360	0.12674720789616187	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5223	0.1235075870973234	No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC	5191	0.12275088734868961	No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT	4752	0.11236991267211964	No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC	4658	0.11014710716050784	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4618	0.10920123247471558	No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT	4580	0.10830265152321294	No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG	4535	0.10723854250169666	No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT	4521	0.10690748636166937	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4409	0.10425903724145107	No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA	4394	0.10390433423427897	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.1823433572403161E-5	0.0
18-19	0.0	0.0	0.0	2.3646867144806322E-5	0.0
20-21	0.0	0.0	0.0	4.7293734289612644E-5	0.0
22-23	0.0	0.0	0.0	1.182343357240316E-4	0.0
24-25	0.0	0.0	0.0	1.8917493715845058E-4	0.0
26-27	0.0	0.0	0.0	3.6652644074449797E-4	0.0
28-29	0.0	0.0	0.0	0.0013005776929643476	0.0
30-31	0.0	0.0	0.0	0.006278243226946078	0.0
32-33	0.0	0.0	0.0	0.01897661088370707	0.0
34-35	0.0	0.0	0.0	0.03874539181676516	0.0
36-37	0.0	0.0	0.0	0.07421569253397464	0.0
38-39	0.0	0.0	0.0	0.1320204592694537	0.0
40-41	0.0	0.0	0.0	0.19811345293918736	0.0
42-43	0.0	0.0	0.0	0.2716197394588178	0.0
44-45	0.0	0.0	0.0	0.360602900524724	0.0
46-47	0.0	0.0	0.0	0.4758459075549376	0.0
48-49	0.0	0.0	0.0	0.6144638427577922	0.0
50-51	0.0	0.0	0.0	0.7652599145402221	0.0
52-53	0.0	0.0	0.0	0.9155121083783215	0.0
54-55	0.0	0.0	0.0	1.0844689741279625	0.0
56-57	0.0	0.0	0.0	1.2953162650246282	0.0
58-59	0.0	0.0	0.0	1.5518020095107699	0.0
60-61	0.0	0.0	0.0	1.8155473422103672	0.0
62-63	0.0	0.0	0.0	2.0972051767721553	0.0
64-65	0.0	0.0	0.0	2.3986199688334295	0.0
66-67	0.0	0.0	0.0	2.7413103674959625	0.0
68-69	0.0	0.0	0.0	3.121469227149441	0.0
70-71	0.0	0.0	0.0	3.552646202667839	0.0
72-73	0.0	0.0	0.0	4.0041949542314885	0.0
74-75	0.0	0.0	0.0	4.451924736751252	0.0
76-77	0.0	0.0	0.0	4.968029435620222	0.0
78-79	0.0	0.0	0.0	5.521342479941545	0.0
80-81	0.0	0.0	0.0	6.116853358682775	0.0
82-83	0.0	0.0	0.0	6.735573637526633	0.0
84-85	0.0	0.0	0.0	7.334927132178894	0.0
86-87	0.0	0.0	0.0	7.979268791574148	0.0
88	0.0	0.0	0.0	8.501025091690728	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6255	0.0	44.44168	94
GTACTAG	6475	0.0	32.023575	1
CAGTACG	710	0.0	31.779427	9
CTGTGCG	9270	0.0	31.43946	9
TAGTACT	9420	0.0	30.591694	4
ACTGTGC	9785	0.0	30.497416	8
TAGGCAT	8015	0.0	29.962835	5
TACCCCG	255	0.0	29.488003	5
CAACGCA	10615	0.0	29.353445	5
GTACTGT	10020	0.0	29.172445	6
TACTGTG	10760	0.0	28.82583	7
GGTATCA	8265	0.0	28.44449	1
GTATCAA	11965	0.0	28.372362	1
TGAACCG	620	0.0	28.046303	5
CCGTGTT	560	0.0	27.700594	9
CTAGTAC	11055	0.0	27.513426	3
CTAGGCA	9010	0.0	27.444473	4
TCAAGAC	1080	0.0	27.423058	3
ATCAACG	11485	0.0	27.138239	3
TCTAACG	3140	0.0	26.650154	2
>>END_MODULE