FastQCFastQC Report
Mon 16 Jan 2017
ERR1146425_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146425_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3950534
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC132220.3346889306610195No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120520.30507268131346293No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT110490.27968370858218156No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA109720.2777346049926415No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC101660.257332299886547No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA101200.25616790033954906No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC94590.23943598510986108No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC88380.22371659122538876No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG63650.16111745905743377No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT59220.14990378515917088No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC58210.14734716876250148No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT53740.13603224272971703No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC53040.13426033037558974No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT50400.1275776894971667No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT49900.1263120378156472No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC49580.12550202073947472No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT48880.1237301083853474No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC47540.12033816187887511No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA47480.12018628367709276No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA47320.11978127513900654No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC46930.1187940668274213No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG43190.10932699224965535No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG43140.10920042708150342No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA41490.10502377653248904No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC41130.10411250732179499No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA40930.10360624664918718No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT40870.10345436844740484No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC40660.10292279474116664No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT40080.101454638790604No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA39800.10074587384895307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG39100.039.2063671
GGGCACC38150.038.60647294
TAGGCAT47000.035.6872675
GCATCGT45500.032.5082248
CTAGGCA53750.031.8195154
CTGTGCG52650.029.433199
TAGTACT54000.029.2358784
CTAACGC20550.028.5822543
CGTTAAC7100.028.5667021
TATTCTC19700.028.1422445
TCTAACG21450.027.8204152
AGGGCTA46650.027.5957285
CAACGCA76000.027.5717435
GTATCAA85600.027.3862571
GGTATCA59150.027.0330451
TAACGCC21850.026.6649554
ACTGTGC61800.026.5174068
CTAGTAC62800.026.4127313
CAATGCG28000.026.245918-19
CTATTGA47850.026.0067239
Produced by FastQC (version 0.11.5)