##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146425_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3950534
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.493920062452318	31.0	31.0	34.0	27.0	34.0
2	30.63402137533812	31.0	31.0	34.0	27.0	34.0
3	30.848711085640574	31.0	31.0	34.0	27.0	34.0
4	34.304214822603726	37.0	35.0	37.0	30.0	37.0
5	34.54757458105664	37.0	35.0	37.0	30.0	37.0
6	34.652416863137994	37.0	35.0	37.0	31.0	37.0
7	34.55336620315127	37.0	35.0	37.0	30.0	37.0
8	34.48358449769069	37.0	35.0	37.0	30.0	37.0
9	35.90328067041063	39.0	35.0	39.0	30.0	39.0
10-11	35.81458645843828	39.0	35.0	39.0	30.0	39.0
12-13	35.79094319907132	39.0	35.0	39.0	30.0	39.0
14-15	36.82735688390481	40.0	36.0	41.0	29.5	41.0
16-17	36.664816832357346	40.0	36.0	41.0	28.5	41.0
18-19	36.6256692133266	40.0	36.0	41.0	28.5	41.0
20-21	36.942387915152736	40.0	36.0	41.0	30.0	41.0
22-23	36.675070003194506	40.0	36.0	41.0	29.0	41.0
24-25	36.78047296897078	40.0	36.0	41.0	29.5	41.0
26-27	36.847015618648015	40.0	36.0	41.0	30.0	41.0
28-29	36.698996262277454	40.0	36.0	41.0	29.5	41.0
30-31	36.64899428786083	40.0	35.5	41.0	29.5	41.0
32-33	36.74640808052785	40.0	35.5	41.0	30.0	41.0
34-35	36.78890043725734	40.0	36.0	41.0	30.0	41.0
36-37	36.71485158715252	40.0	35.0	41.0	30.0	41.0
38-39	36.55220673964583	39.0	35.0	41.0	29.0	41.0
40-41	36.513516400567624	39.0	35.0	41.0	29.0	41.0
42-43	36.35628411247694	39.0	35.0	41.0	29.0	41.0
44-45	36.17122723155907	39.0	35.0	41.0	28.0	41.0
46-47	36.04466459471048	39.0	35.0	41.0	28.0	41.0
48-49	35.91152006285732	38.5	34.5	40.5	28.0	41.0
50-51	35.70067223823412	38.0	34.0	40.0	27.5	41.0
52-53	35.512466795628136	38.0	34.0	40.0	27.0	41.0
54-55	35.31719952289994	38.0	34.0	40.0	27.0	41.0
56-57	35.1595498228847	37.0	33.5	40.0	27.0	41.0
58-59	34.89206851529439	37.0	33.0	40.0	26.5	41.0
60-61	34.63512907368978	36.0	33.0	40.0	26.0	41.0
62-63	34.35897703955972	36.0	33.0	39.0	26.0	41.0
64-65	34.07573824196932	35.0	33.0	39.0	26.0	41.0
66-67	33.788280014803064	35.0	32.0	39.0	26.0	41.0
68-69	33.50344598983327	35.0	32.0	38.0	26.0	40.0
70-71	33.430729744383925	35.0	32.0	37.5	26.0	40.0
72-73	33.21972232108368	35.0	32.0	37.0	25.5	40.0
74-75	32.94938190634481	35.0	32.0	36.5	25.0	39.0
76-77	32.71961309534357	35.0	32.0	36.0	25.0	39.0
78-79	32.5064081210287	35.0	32.0	36.0	25.0	39.0
80-81	32.29502214131051	35.0	31.5	35.5	24.5	37.5
82-83	32.06463265472465	35.0	31.0	35.0	24.0	37.0
84-85	31.875070686646414	35.0	31.0	35.0	24.0	37.0
86-87	31.707742927918098	35.0	31.0	35.0	24.0	36.0
88-89	31.709096162695978	35.0	31.5	35.0	24.0	36.0
90-91	31.63554104331212	35.0	32.0	35.0	24.0	36.0
92-93	31.49637378136728	35.0	31.0	35.0	24.0	36.0
94-95	31.465925872299795	35.0	31.0	35.0	24.0	35.0
96-97	31.377176477914126	35.0	31.0	35.0	23.0	35.0
98-99	31.30008576055794	35.0	31.0	35.0	23.0	35.0
100	30.805792837120247	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6634743947398576
1101	2	0.7839231017380648
1101	3	0.8247398784412461
1101	4	0.8614029987691509
1101	5	0.8344979835273278
1101	6	0.5122432538898636
1101	7	0.5998914240884261
1101	8	0.6139012051009516
1101	9	0.7236917854628047
1101	10-11	0.7605826141782259
1101	12-13	0.6921162267840373
1101	14-15	0.8932983615238115
1101	16-17	1.077402605789139
1101	18-19	1.193490180964666
1101	20-21	0.8821337109453609
1101	22-23	1.1363590642918453
1101	24-25	1.0703350507153786
1101	26-27	0.9301251427720061
1101	28-29	1.1082151253955246
1101	30-31	1.0751304940522033
1101	32-33	0.9635879643358649
1101	34-35	0.8454729909366065
1101	36-37	0.8028732320118905
1101	38-39	0.9709071265087914
1101	40-41	0.8284562943728844
1101	42-43	0.8208139496088407
1101	44-45	0.8774436039986853
1101	46-47	0.8231591790750059
1101	48-49	0.7857042794187095
1101	50-51	0.8012967703642531
1101	52-53	0.8744954387064965
1101	54-55	0.8861708352689845
1101	56-57	0.7257340953354685
1101	58-59	0.6945536126046647
1101	60-61	0.7299514754499583
1101	62-63	0.7116572124388085
1101	64-65	0.7008057195789377
1101	66-67	0.7250011312382227
1101	68-69	0.7776706334520966
1101	70-71	0.7267553616416862
1101	72-73	0.6497899323789653
1101	74-75	0.657305067857429
1101	76-77	0.6505302520295757
1101	78-79	0.6658191432959271
1101	80-81	0.6371737392362746
1101	82-83	0.6666706141726451
1101	84-85	0.664669546421397
1101	86-87	0.6735165256171491
1101	88-89	0.7080270448234742
1101	90-91	0.7269453010459266
1101	92-93	0.7409810314643721
1101	94-95	0.6648180734837261
1101	96-97	0.6589629796978933
1101	98-99	0.6259502644379111
1101	100	0.6836655535284955
1102	1	0.6916948517173367
1102	2	0.8779378884494804
1102	3	0.8401215239492963
1102	4	0.8741224890167274
1102	5	0.8064685925063628
1102	6	0.5768993959687592
1102	7	0.6073461884252112
1102	8	0.6154130125818611
1102	9	0.8256548855649513
1102	10-11	0.8005047589809848
1102	12-13	0.6865590498785465
1102	14-15	0.9622732478266443
1102	16-17	1.134091282281183
1102	18-19	1.1990723621262092
1102	20-21	0.9120814376100839
1102	22-23	1.1742359521511858
1102	24-25	1.015496765171335
1102	26-27	0.8565642175081294
1102	28-29	1.0105982817990409
1102	30-31	1.0851442805933758
1102	32-33	0.9221275637813662
1102	34-35	0.8556434910825317
1102	36-37	0.7816159663374904
1102	38-39	0.8008893575268345
1102	40-41	0.8133859480525345
1102	42-43	0.8397871638945489
1102	44-45	0.9394897273509883
1102	46-47	0.8020469988862828
1102	48-49	0.8197024675601554
1102	50-51	0.9176230295451475
1102	52-53	0.9115303899443958
1102	54-55	0.9497110579208581
1102	56-57	0.7489296848582967
1102	58-59	0.748670726763919
1102	60-61	0.7837650425008817
1102	62-63	0.8024047256933429
1102	64-65	0.7512246358686383
1102	66-67	0.8109036708802293
1102	68-69	0.7799542288361181
1102	70-71	0.7438921691225957
1102	72-73	0.6861024202188446
1102	74-75	0.7495389283321643
1102	76-77	0.7466900087054853
1102	78-79	0.6863325133528448
1102	80-81	0.6719386232286837
1102	82-83	0.6795192297424677
1102	84-85	0.711784024873662
1102	86-87	0.7307117472962155
1102	88-89	0.7079267997969652
1102	90-91	0.7399153658593853
1102	92-93	0.7357760421652841
1102	94-95	0.7246251379080668
1102	96-97	0.7380278813224912
1102	98-99	0.7347357264010412
1102	100	0.7688578658977754
1103	1	0.48588823901737044
1103	2	0.6612468508206533
1103	3	0.5965551117875307
1103	4	0.5945765945900732
1103	5	0.5494536779537853
1103	6	0.3798371806264669
1103	7	0.4284202200129599
1103	8	0.4094374590404186
1103	9	0.5259344515682329
1103	10-11	0.6620460202568879
1103	12-13	0.535336016966653
1103	14-15	0.6248054390828486
1103	16-17	0.8461442411558906
1103	18-19	1.0128635485120512
1103	20-21	0.6583976475036692
1103	22-23	0.8301241071403567
1103	24-25	0.7873006181073379
1103	26-27	0.6078365656231028
1103	28-29	0.8107537241305707
1103	30-31	0.7985554471023235
1103	32-33	0.5968512775940766
1103	34-35	0.6253008009137204
1103	36-37	0.6079377476186778
1103	38-39	0.684566774978336
1103	40-41	0.6488484607589271
1103	42-43	0.6531101358757283
1103	44-45	0.6896519211423993
1103	46-47	0.6691397704275914
1103	48-49	0.6446967998176305
1103	50-51	0.6496231016900751
1103	52-53	0.7427004558674852
1103	54-55	0.7213753505519804
1103	56-57	0.6087691350539757
1103	58-59	0.5622183688970281
1103	60-61	0.5634927103936178
1103	62-63	0.5068193514104422
1103	64-65	0.5332890982431877
1103	66-67	0.5696877677922103
1103	68-69	0.5046691736886828
1103	70-71	0.517711978876072
1103	72-73	0.48463072102897087
1103	74-75	0.4838324903631488
1103	76-77	0.5153671551725196
1103	78-79	0.5234766952106256
1103	80-81	0.5457048556719712
1103	82-83	0.42968051951736896
1103	84-85	0.4188165831826218
1103	86-87	0.4829241072127992
1103	88-89	0.4726244766112444
1103	90-91	0.46834247811711904
1103	92-93	0.5320337070677077
1103	94-95	0.5098334940898646
1103	96-97	0.6122160800087393
1103	98-99	0.5714183196337004
1103	100	0.5977459295592347
1104	1	0.4804684163318811
1104	2	0.6038171196863011
1104	3	0.6409944071438503
1104	4	0.7193266667059319
1104	5	0.644759268846002
1104	6	0.3197073496068512
1104	7	0.38186640691058926
1104	8	0.46201385527689354
1104	9	0.5209672112649599
1104	10-11	0.5652497154962361
1104	12-13	0.4768258692768015
1104	14-15	0.6803327054865775
1104	16-17	0.8097409259007478
1104	18-19	0.7838975624896491
1104	20-21	0.6995874674260918
1104	22-23	0.8214253746485198
1104	24-25	0.6501906202387673
1104	26-27	0.5666323093137393
1104	28-29	0.7776625521034148
1104	30-31	0.796512054164225
1104	32-33	0.6514120658829157
1104	34-35	0.5225723634249988
1104	36-37	0.5457159949369128
1104	38-39	0.649994898751487
1104	40-41	0.5039293355788672
1104	42-43	0.45877231807865115
1104	44-45	0.569005251613568
1104	46-47	0.5408200456188297
1104	48-49	0.5365286854819686
1104	50-51	0.6006228330477441
1104	52-53	0.5384091291826891
1104	54-55	0.5501024193258672
1104	56-57	0.45882257941732973
1104	58-59	0.5034729444388404
1104	60-61	0.4900076027325255
1104	62-63	0.4399338198679601
1104	64-65	0.4641328584799709
1104	66-67	0.5125732586353209
1104	68-69	0.47404490599670623
1104	70-71	0.4050140104225832
1104	72-73	0.4196083092815428
1104	74-75	0.414129451797713
1104	76-77	0.38874557231816453
1104	78-79	0.4240264407536145
1104	80-81	0.4628188190871896
1104	82-83	0.39741995178481204
1104	84-85	0.363686928571024
1104	86-87	0.3569124864829156
1104	88-89	0.31848096267177084
1104	90-91	0.37660138642498353
1104	92-93	0.3837133096620562
1104	94-95	0.30825597690263606
1104	96-97	0.34134479747747903
1104	98-99	0.3366978972798549
1104	100	0.43432338662238124
1105	1	0.3998091880911616
1105	2	0.5201764710103021
1105	3	0.47728742261615054
1105	4	0.6304955414465994
1105	5	0.5086874863724944
1105	6	0.2899695566751248
1105	7	0.3754267640105553
1105	8	0.3820072997400601
1105	9	0.4194680283498968
1105	10-11	0.4387637687155106
1105	12-13	0.4061024502042301
1105	14-15	0.6307267644887702
1105	16-17	0.7968433065977649
1105	18-19	0.7503695797367556
1105	20-21	0.5472959039672247
1105	22-23	0.825204433414342
1105	24-25	0.5764891200475191
1105	26-27	0.4740019584353732
1105	28-29	0.7094809422568602
1105	30-31	0.7034525028871883
1105	32-33	0.5298287924281126
1105	34-35	0.5131509072289049
1105	36-37	0.42421476390767054
1105	38-39	0.589078266820259
1105	40-41	0.44676828898204235
1105	42-43	0.5101225936639437
1105	44-45	0.5003041161280635
1105	46-47	0.44585263145596343
1105	48-49	0.5762384962500988
1105	50-51	0.5953092928260659
1105	52-53	0.519765822803528
1105	54-55	0.5201681960057343
1105	56-57	0.44831289886946024
1105	58-59	0.4478386530359373
1105	60-61	0.4700430073457298
1105	62-63	0.4379946263351826
1105	64-65	0.3436067078987861
1105	66-67	0.42187191964793413
1105	68-69	0.3887195066177185
1105	70-71	0.34599109838921294
1105	72-73	0.2586225731396752
1105	74-75	0.3157596014004085
1105	76-77	0.378754926333265
1105	78-79	0.4157208728556796
1105	80-81	0.44523191533690465
1105	82-83	0.3958929674221139
1105	84-85	0.3726009068586542
1105	86-87	0.39724602921103624
1105	88-89	0.44113435614423224
1105	90-91	0.41077513407976696
1105	92-93	0.44397687602861424
1105	94-95	0.38274774928486366
1105	96-97	0.37947108466944357
1105	98-99	0.4030696302660033
1105	100	0.49973136664619844
1106	1	0.5089500607179538
1106	2	0.5700774279059466
1106	3	0.4786812799001581
1106	4	0.6280309332687537
1106	5	0.5245805314583833
1106	6	0.31385622002073177
1106	7	0.3035653315185485
1106	8	0.3382049675006229
1106	9	0.5289403303168072
1106	10-11	0.44196353697210355
1106	12-13	0.38449136933992634
1106	14-15	0.5377614435822053
1106	16-17	0.7200927161758202
1106	18-19	0.6623021457076348
1106	20-21	0.5470061570430929
1106	22-23	0.7927880996995
1106	24-25	0.5996812907364628
1106	26-27	0.4908028817279515
1106	28-29	0.6689582921267458
1106	30-31	0.689742506672431
1106	32-33	0.4845637863185246
1106	34-35	0.47324432456042587
1106	36-37	0.352994875224077
1106	38-39	0.5528360489476256
1106	40-41	0.46594862863148023
1106	42-43	0.42075891093046636
1106	44-45	0.4296296851640946
1106	46-47	0.4766877400136167
1106	48-49	0.46637437280627125
1106	50-51	0.44974018777866576
1106	52-53	0.4606865806060938
1106	54-55	0.4994734854637173
1106	56-57	0.5077397088099502
1106	58-59	0.48077693044513126
1106	60-61	0.45667391374745847
1106	62-63	0.4320808320674132
1106	64-65	0.46079404613414
1106	66-67	0.5054702678874676
1106	68-69	0.41428520669892777
1106	70-71	0.46085278111105055
1106	72-73	0.4008946396821358
1106	74-75	0.4087177496834329
1106	76-77	0.34712588955029133
1106	78-79	0.4084469191012374
1106	80-81	0.4035998092123947
1106	82-83	0.4645281403168582
1106	84-85	0.42437165167606494
1106	86-87	0.40497104625278624
1106	88-89	0.4253429796135677
1106	90-91	0.3776093627053818
1106	92-93	0.5216156876088007
1106	94-95	0.4679352396339631
1106	96-97	0.37761073619091334
1106	98-99	0.3738876084333498
1106	100	0.47912272758655305
1107	1	0.41635986784677215
1107	2	0.5143380614314701
1107	3	0.5063351275492138
1107	4	0.5978903781797484
1107	5	0.5545601503922271
1107	6	0.23788515843151004
1107	7	0.2494437565847889
1107	8	0.3356775035018984
1107	9	0.38479385695745805
1107	10-11	0.41256614463944175
1107	12-13	0.29581209921256857
1107	14-15	0.5061970912053226
1107	16-17	0.5894081341799549
1107	18-19	0.6815586443583541
1107	20-21	0.450945038089543
1107	22-23	0.6982674741636643
1107	24-25	0.38484274035459975
1107	26-27	0.34447617631678185
1107	28-29	0.4878982203032365
1107	30-31	0.45716081485304727
1107	32-33	0.36528598975324655
1107	34-35	0.24057390884770768
1107	36-37	0.1931959325537207
1107	38-39	0.3142890948141499
1107	40-41	0.13925565343195245
1107	42-43	0.29491028622395987
1107	44-45	0.22403709106973224
1107	46-47	0.23644555687774726
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2216	94-95	-0.09116576204816695
2216	96-97	-0.2295858837151492
2216	98-99	-0.16885804110638247
2216	100	-0.130374742110007
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16078.0
1	2766.0
2	420.0
3	401.0
4	334.0
5	241.0
6	214.0
7	260.0
8	502.0
9	817.0
10	1476.0
11	2695.0
12	4512.0
13	6466.0
14	8467.0
15	10558.0
16	12912.0
17	14980.0
18	17423.0
19	19735.0
20	22567.0
21	25802.0
22	29719.0
23	34630.0
24	39331.0
25	44660.0
26	51328.0
27	58782.0
28	69673.0
29	83794.0
30	98983.0
31	119362.0
32	147430.0
33	187021.0
34	259508.0
35	351604.0
36	522619.0
37	797415.0
38	658144.0
39	226711.0
40	194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47131108036732	21.086148192143597	12.295192830601719	24.147347896887357
2	15.49247036467035	19.915338162992217	40.76793661117212	23.82425486116532
3	18.385512560987674	26.97423556051689	28.980753290466783	25.65949858802865
4	12.047861870714357	16.745119161328844	40.191174869974844	31.015844097981955
5	13.046485786215262	38.666558538706546	35.20752306214315	13.079432612935035
6	29.645532728518166	38.652211945264966	18.402019204624047	13.300236121592828
7	26.693999518164365	31.79960013239032	24.103026329748058	17.403374019697257
8	25.958073404741665	35.11826183807728	20.2517244265501	18.67194033063095
9	26.309696225180325	14.662806591507497	21.640609971939988	37.38688721137219
10-11	25.565289508363637	25.769317830319437	27.881834837451073	20.783557823865856
12-13	25.707529374491443	23.760940085516687	27.72691154475248	22.80461899523939
14-15	23.223976413838802	24.893059681476892	25.006056346414873	26.87690755826943
16-17	22.225765192513922	27.384239454347515	26.491334595908828	23.898660757229738
18-19	21.721470681382186	28.077738829728826	27.923817056395272	22.27697343249372
20-21	23.420027333122203	27.211368807693383	26.665329826502997	22.703274032681417
22-23	23.911837110552746	26.87694958132294	26.374664298392386	22.83654900973193
24-25	23.264516084705996	26.992386008006253	27.200742428790033	22.542355478497722
26-27	23.143595135283316	27.19063947198196	26.211590115626386	23.45417527710834
28-29	22.37578334896607	27.71406301343618	26.742068490343218	23.16808514725453
30-31	23.160044618549065	27.302246906267463	26.648413161250662	22.889295313932813
32-33	23.551705041264263	27.609799812752644	25.6749613336368	23.16353381234629
34-35	23.5102957364094	27.3364840649604	26.78908229883548	22.364137899794724
36-37	23.229672695359845	26.96632473698598	26.488363853497106	23.315638714157068
38-39	23.440424532065325	26.969169813207877	27.001974517438093	22.588431137288705
40-41	22.938674474395903	26.23385825448996	27.61554942126666	23.21191784984747
42-43	22.926128388690955	26.476232239109702	26.91007273983135	23.68756663236799
44-45	22.96697933416522	27.433311846855073	26.83549990423352	22.76420891474619
46-47	23.024028349764688	27.067008083516047	26.43959671884511	23.469366847874156
48-49	23.620219682912076	26.63752319507784	26.647332399062844	23.09492472294724
50-51	23.217429566263675	26.53427792982101	26.56962649620633	23.67866600770899
52-53	22.653546147342464	27.18350977708986	26.462008449706055	23.70093562586162
54-55	22.713268976736213	27.114340464069404	26.383575006721927	23.788815552472453
56-57	23.752313558102543	26.542057168725265	26.164012200547937	23.541617072624256
58-59	22.783898835607864	26.75191953184087	27.133319286116365	23.33086234643491
60-61	22.81527704867516	27.516769642957566	26.349173471176773	23.3187798371905
62-63	23.200078675213224	26.76270199485685	25.97831622575766	24.058903104172266
64-65	22.317719436936276	26.985220028916366	26.442214281651722	24.25484625249564
66-67	23.14454379976726	26.480411699006556	26.37103856382742	24.004005937398766
68-69	22.49238388624024	26.61513917208663	26.66529391237772	24.227183029295407
70-71	22.540858294249954	26.289592397455518	26.802593136857585	24.36695617143694
72-73	23.182017265367545	26.41616249326546	27.13267702778741	23.269143213579586
74-75	23.246186684650354	26.749531586498236	26.765004342169302	23.239277386682105
76-77	22.96492340309619	26.642788539771367	26.431653887263128	23.96063416986931
78-79	22.81544934160775	26.65729469801618	26.48139847487882	24.045857485497248
80-81	22.39655412182285	26.97490824270417	27.153352597265535	23.47518503820745
82-83	23.216744050172807	25.91729100218893	28.093787995148833	22.772176952489428
84-85	23.484097938164005	26.516347415763846	26.684780271279074	23.31477437479307
86-87	22.128628048827185	27.171634426136603	27.227003828632085	23.47273369640413
88-89	23.27424264026254	25.881733803663032	26.963012601481072	23.881010954593354
90-91	22.877951777980456	25.96817268391477	27.233671489875334	23.920204048229444
92-93	23.26730633335543	26.15729821744248	27.038595248339004	23.536800200863087
94-95	22.043553416802546	26.178800109906717	27.741937795715042	24.03570867757569
96-97	22.480942557209442	26.107733657384617	27.166304877366798	24.245018908039146
98-99	21.837671541040095	26.83701381029817	27.084699340273033	24.2406153083887
100	22.666163373937966	26.07416316920872	26.3295884942164	24.93008496263691
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13555.0
1	7996.0
2	2260.0
3	1620.5
4	859.0
5	467.0
6	364.5
7	331.0
8	311.0
9	319.0
10	306.5
11	297.0
12	284.5
13	277.0
14	298.0
15	385.5
16	450.0
17	481.0
18	586.0
19	730.0
20	930.0
21	1257.5
22	1832.5
23	2593.5
24	3389.0
25	4590.0
26	6843.5
27	9565.5
28	13361.5
29	21350.5
30	32503.0
31	43480.0
32	57658.5
33	70170.5
34	79678.0
35	91175.0
36	101588.5
37	119733.5
38	127542.5
39	119032.5
40	110197.0
41	100935.5
42	98719.5
43	104894.0
44	115832.0
45	138159.0
46	160145.5
47	174095.5
48	182638.5
49	179372.0
50	171494.0
51	174928.0
52	177789.0
53	172008.5
54	163422.5
55	142731.0
56	126307.0
57	107039.0
58	86981.0
59	81716.0
60	67937.0
61	48311.0
62	36305.0
63	29790.5
64	22240.5
65	12525.5
66	7839.5
67	5651.5
68	4118.5
69	3055.5
70	2261.5
71	1694.5
72	1315.0
73	1133.5
74	931.5
75	722.0
76	521.0
77	328.0
78	224.5
79	160.0
80	98.0
81	64.5
82	42.0
83	30.0
84	21.0
85	15.0
86	16.0
87	12.5
88	11.5
89	15.0
90	9.5
91	5.0
92	5.0
93	5.0
94	5.5
95	4.0
96	2.5
97	2.5
98	3.0
99	3.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9961944385240071
2	0.4423452626910691
3	0.47580909315044495
4	0.4611528466784491
5	0.42837246812709373
6	0.4830992468359973
7	0.4995020926284902
8	0.5013752571171392
9	0.516031503589135
10-11	0.5298650764681433
12-13	0.5288272420892973
14-15	0.5258023345704657
16-17	0.5315737062381946
18-19	0.5300675807371864
20-21	0.5282703553494288
22-23	0.4911108219800159
24-25	0.524726530641174
26-27	0.5059189466537941
28-29	0.5116650052878927
30-31	0.5146139737058332
32-33	0.5173604378547305
34-35	0.5308269717460982
36-37	0.5481790562997306
38-39	0.5371299171200653
40-41	0.5426101889010447
42-43	0.5398004421680714
44-45	0.5494573644980653
46-47	0.5470779393368087
48-49	0.5202208106549646
50-51	0.5340037574667121
52-53	0.5268021993988661
54-55	0.5373830474563692
56-57	0.5242455830021967
58-59	0.5298017938840673
60-61	0.5230938399720139
62-63	0.517714820325556
64-65	0.5296878852327306
66-67	0.5282070727653527
68-69	0.5491156385440551
70-71	0.5317635539904225
72-73	0.5357503567872091
74-75	0.5327507623020078
76-77	0.5208789495293548
78-79	0.5517481940416157
80-81	0.5249290349102171
82-83	0.5253973260323794
84-85	0.5334721837604739
86-87	0.8405952207979985
88-89	1.1079767950358104
90-91	1.0990539506810977
92-93	1.0984590943907837
94-95	1.1032559142637426
96-97	1.0969023428225146
98-99	1.092510531487642
100	1.0867897858871738
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3950534.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.41631888599915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.66739431979956	21.67023562811866
2	12.583777921227085	7.403368482428228
3	5.0313650354886015	4.440127149473974
4	2.5428557158336855	2.992058184721973
5	1.4869606701125038	2.18704546214842
6	0.9615318721025159	1.6970836901291615
7	0.6380912035023393	1.3139206024403052
8	0.4480513745330175	1.0544017688458784
9	0.3386590097379123	0.8965891281660407
>10	1.7870677824195897	9.783136370761822
>50	0.2022866569698343	4.180532141499028
>100	0.23660548417612456	15.197672263576386
>500	0.04676189037133911	9.753043384977484
>1k	0.027385416460545927	14.398366683559852
>5k	6.88941294604929E-4	1.320413508489493
>10k+	5.167059709536967E-4	1.7120055506634004
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	13222	0.3346889306610195	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12052	0.30507268131346293	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	11049	0.27968370858218156	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	10972	0.2777346049926415	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10166	0.257332299886547	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10120	0.25616790033954906	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	9459	0.23943598510986108	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8838	0.22371659122538876	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6365	0.16111745905743377	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5922	0.14990378515917088	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5821	0.14734716876250148	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5374	0.13603224272971703	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5304	0.13426033037558974	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5040	0.1275776894971667	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4990	0.1263120378156472	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4958	0.12550202073947472	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4888	0.1237301083853474	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4754	0.12033816187887511	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4748	0.12018628367709276	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4732	0.11978127513900654	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4693	0.1187940668274213	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4319	0.10932699224965535	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4314	0.10920042708150342	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4149	0.10502377653248904	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4113	0.10411250732179499	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4093	0.10360624664918718	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4087	0.10345436844740484	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4066	0.10292279474116664	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4008	0.101454638790604	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	3980	0.10074587384895307	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.531303363039022E-5	0.0
12-13	0.0	0.0	0.0	2.531303363039022E-5	8.859561770636578E-5
14-15	0.0	0.0	0.0	2.531303363039022E-5	1.392216849671462E-4
16-17	0.0	0.0	0.0	2.531303363039022E-5	1.7719123541273154E-4
18-19	0.0	0.0	0.0	2.531303363039022E-5	2.0250426904312176E-4
20-21	0.0	0.0	0.0	5.062606726078044E-5	2.27817302673512E-4
22-23	0.0	0.0	0.0	1.265651681519511E-4	2.784433699342924E-4
24-25	0.0	0.0	0.0	2.27817302673512E-4	2.784433699342924E-4
26-27	0.0	0.0	0.0	5.442302230533897E-4	3.1641292037987774E-4
28-29	0.0	0.0	0.0	0.0014554994337474378	3.2906943719507287E-4
30-31	0.0	0.0	0.0	0.0067079539120534085	3.41725954010268E-4
32-33	0.0	0.0	0.0	0.01936447072724852	3.670389876406582E-4
34-35	0.0	0.0	0.0	0.03899472830761613	3.796955044558533E-4
36-37	0.0	0.0	0.0	0.07328123235997969	3.796955044558533E-4
38-39	0.0	0.0	0.0	0.12772956769894905	3.796955044558533E-4
40-41	0.0	0.0	0.0	0.18989837829518744	3.796955044558533E-4
42-43	0.0	0.0	0.0	0.25586414393598433	3.796955044558533E-4
44-45	0.0	0.0	0.0	0.33665069076737475	3.796955044558533E-4
46-47	0.0	0.0	0.0	0.43887737708370567	3.923520212710484E-4
48-49	0.0	0.0	0.0	0.5609241687326321	4.303215717166337E-4
50-51	0.0	0.0	0.0	0.6934378997877249	4.303215717166337E-4
52-53	0.0	0.0	0.0	0.8237367404001585	4.55634605347024E-4
54-55	0.0	1.265651681519511E-5	0.0	0.9702865486033028	4.55634605347024E-4
56-57	0.0	2.531303363039022E-5	0.0	1.1502369046817469	4.55634605347024E-4
58-59	0.0	2.531303363039022E-5	0.0	1.371194375241423	4.55634605347024E-4
60-61	0.0	2.531303363039022E-5	0.0	1.6031123893630583	4.6829112216221905E-4
62-63	0.0	2.531303363039022E-5	0.0	1.8439405913225908	4.809476389774142E-4
64-65	0.0	2.531303363039022E-5	0.0	2.0979568838035565	4.809476389774142E-4
66-67	0.0	2.531303363039022E-5	0.0	2.392777786496712	4.809476389774142E-4
68-69	0.0	2.531303363039022E-5	0.0	2.731086480966877	5.062606726078044E-4
70-71	0.0	2.531303363039022E-5	0.0	3.10266915814419	5.062606726078044E-4
72-73	0.0	2.531303363039022E-5	0.0	3.4898320075210085	5.6954325668378E-4
74-75	0.0	2.531303363039022E-5	0.0	3.8672366824333118	6.328258407597555E-4
76-77	0.0	2.531303363039022E-5	0.0	4.303000556380479	6.581388743901457E-4
78-79	0.0	2.531303363039022E-5	0.0	4.777468058748513	6.581388743901457E-4
80-81	0.0	2.531303363039022E-5	0.0	5.294372861997896	6.581388743901457E-4
82-83	0.0	3.796955044558533E-5	0.0	5.839666232463763	6.83451908020536E-4
84-85	0.0	5.062606726078044E-5	0.0	6.3605704950267485	6.96108424835731E-4
86-87	0.0	5.062606726078044E-5	0.0	6.922709689373638	7.593910089117065E-4
88	0.0	5.062606726078044E-5	0.0	7.3682697073357675	8.10017076172487E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	3910	0.0	39.206367	1
GGGCACC	3815	0.0	38.606472	94
TAGGCAT	4700	0.0	35.687267	5
GCATCGT	4550	0.0	32.508224	8
CTAGGCA	5375	0.0	31.819515	4
CTGTGCG	5265	0.0	29.43319	9
TAGTACT	5400	0.0	29.235878	4
CTAACGC	2055	0.0	28.582254	3
CGTTAAC	710	0.0	28.566702	1
TATTCTC	1970	0.0	28.142244	5
TCTAACG	2145	0.0	27.820415	2
AGGGCTA	4665	0.0	27.595728	5
CAACGCA	7600	0.0	27.571743	5
GTATCAA	8560	0.0	27.386257	1
GGTATCA	5915	0.0	27.033045	1
TAACGCC	2185	0.0	26.664955	4
ACTGTGC	6180	0.0	26.517406	8
CTAGTAC	6280	0.0	26.412731	3
CAATGCG	2800	0.0	26.2459	18-19
CTATTGA	4785	0.0	26.006723	9
>>END_MODULE