##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146425_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3950534
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.829830093855666	31.0	31.0	34.0	30.0	34.0
2	31.821843831745277	31.0	31.0	34.0	30.0	34.0
3	31.999507155235218	33.0	31.0	34.0	30.0	34.0
4	35.73060755837059	37.0	35.0	37.0	35.0	37.0
5	35.65310132756736	37.0	35.0	37.0	33.0	37.0
6	35.68438545270082	37.0	35.0	37.0	33.0	37.0
7	35.60946545454362	37.0	35.0	37.0	33.0	37.0
8	35.5889702506041	37.0	35.0	37.0	33.0	37.0
9	37.191715600979514	39.0	37.0	39.0	33.0	39.0
10-11	37.13368888864139	39.0	37.0	39.0	33.0	39.0
12-13	37.072774465426704	39.0	37.0	39.0	33.0	39.0
14-15	38.26896085440601	40.0	38.0	41.0	33.0	41.0
16-17	38.268350050904516	40.0	38.0	41.0	33.0	41.0
18-19	38.324622190316546	40.0	38.0	41.0	33.0	41.0
20-21	38.338271104615224	40.0	38.0	41.0	33.0	41.0
22-23	38.30226280801532	40.0	38.0	41.0	33.0	41.0
24-25	38.233482992425834	40.0	37.0	41.0	33.0	41.0
26-27	38.09097086621708	40.0	37.0	41.0	33.0	41.0
28-29	38.02658425417931	40.0	37.0	41.0	33.0	41.0
30-31	37.898217937119384	40.0	37.0	41.0	33.0	41.0
32-33	37.77589232240502	40.0	36.5	41.0	33.0	41.0
34-35	37.679044782300316	40.0	36.0	41.0	32.0	41.0
36-37	37.595311798354345	40.0	36.0	41.0	32.0	41.0
38-39	37.4769696957424	40.0	36.0	41.0	32.0	41.0
40-41	37.3532322465773	40.0	36.0	41.0	31.0	41.0
42-43	37.23232783213611	39.5	36.0	41.0	31.0	41.0
44-45	37.03554595404064	39.0	35.0	41.0	31.0	41.0
46-47	36.80044153524561	39.0	35.0	41.0	30.5	41.0
48-49	36.543554491620625	39.0	35.0	41.0	30.0	41.0
50-51	36.34024805254175	39.0	35.0	41.0	30.0	41.0
52-53	36.1457794060246	38.5	35.0	41.0	29.0	41.0
54-55	35.94526018507878	38.0	35.0	40.0	29.0	41.0
56-57	35.686494408097744	38.0	34.0	40.0	28.5	41.0
58-59	35.40385552940438	37.0	34.0	40.0	28.0	41.0
60-61	35.13958555982558	37.0	34.0	40.0	28.0	41.0
62-63	34.86026724488386	36.0	34.0	39.5	27.5	41.0
64-65	34.59082683505572	36.0	33.5	39.0	27.0	41.0
66-67	34.36268514585623	35.0	33.0	39.0	27.0	41.0
68-69	34.169781857338776	35.0	33.0	38.5	27.0	41.0
70-71	33.968001920753	35.0	33.0	38.0	26.5	40.0
72-73	33.76597176482977	35.0	33.0	37.0	26.0	40.0
74-75	33.5802249771803	35.0	33.0	37.0	26.5	39.5
76-77	33.41930268161215	35.0	33.0	36.0	27.0	39.0
78-79	33.3016473216026	35.0	33.0	36.0	27.0	39.0
80-81	33.15332648700151	35.0	33.0	36.0	27.0	38.0
82-83	32.96259062698866	35.0	33.0	35.0	27.0	37.0
84-85	32.81670161552843	35.0	33.0	35.0	27.0	37.0
86-87	32.679789365184554	35.0	33.0	35.0	27.0	36.5
88-89	32.569990284857695	35.0	33.0	35.0	26.0	36.0
90-91	32.480208498395406	35.0	33.0	35.0	26.0	36.0
92-93	32.401553055865364	35.0	33.0	35.0	26.0	36.0
94-95	32.355533327899465	35.0	33.0	35.0	26.0	35.0
96-97	32.2979685784251	35.0	33.0	35.0	26.0	35.0
98-99	32.25892828665694	35.0	33.0	35.0	26.0	35.0
100	31.762504258917907	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4839197915697113
1101	2	0.6010717400616166
1101	3	0.5226382745827252
1101	4	0.33915997646393237
1101	5	0.3951499666715321
1101	6	0.38976110041208045
1101	7	0.40180705960506913
1101	8	0.4390205818827937
1101	9	0.45554659855546475
1101	10-11	0.5012899297215512
1101	12-13	0.5353516416780195
1101	14-15	0.6889511836094684
1101	16-17	0.6548345695450877
1101	18-19	0.6914828880098298
1101	20-21	0.661450846482218
1101	22-23	0.6444954245408425
1101	24-25	0.7173124386248375
1101	26-27	0.7181063420334155
1101	28-29	0.7648890074832835
1101	30-31	0.7859812246823594
1101	32-33	0.812727172011769
1101	34-35	0.7976810947232167
1101	36-37	0.8232451847643176
1101	38-39	0.8169660477453533
1101	40-41	0.8210627348253823
1101	42-43	0.8408947847408967
1101	44-45	0.834585213159464
1101	46-47	0.8347801938177142
1101	48-49	0.9231435819667908
1101	50-51	0.8343599713536065
1101	52-53	0.8441437518332151
1101	54-55	0.8577877225518407
1101	56-57	0.838537265092846
1101	58-59	0.8039189345316302
1101	60-61	0.8429620039609773
1101	62-63	0.7955763876607662
1101	64-65	0.7834724567573517
1101	66-67	0.7366292745401708
1101	68-69	0.7850806949840319
1101	70-71	0.7657935232504371
1101	72-73	0.657417015741153
1101	74-75	0.6266346389939272
1101	76-77	0.6652416775861596
1101	78-79	0.6151892612831489
1101	80-81	0.6543966226577922
1101	82-83	0.6469762236961074
1101	84-85	0.6378879196578922
1101	86-87	0.6444860591446826
1101	88-89	0.5676837123470762
1101	90-91	0.5583822338424014
1101	92-93	0.5875956755134482
1101	94-95	0.5558273559711466
1101	96-97	0.5748419190499447
1101	98-99	0.5486646276598393
1101	100	0.6215113160443195
1102	1	0.46726878106336756
1102	2	0.5492419453245212
1102	3	0.47680509325095244
1102	4	0.33883970918439843
1102	5	0.34172263557566396
1102	6	0.3520516422672273
1102	7	0.40609775954183647
1102	8	0.40434750683050424
1102	9	0.4379958908362198
1102	10-11	0.48828574055865914
1102	12-13	0.5122049206555914
1102	14-15	0.609035073268501
1102	16-17	0.6304196235360422
1102	18-19	0.6539907920907666
1102	20-21	0.6676534219965902
1102	22-23	0.6139605918641564
1102	24-25	0.6255513029908073
1102	26-27	0.6465487212833807
1102	28-29	0.6257905591151669
1102	30-31	0.6807347389583498
1102	32-33	0.6728406715740078
1102	34-35	0.6517247161298982
1102	36-37	0.6423848407543957
1102	38-39	0.6329215751299557
1102	40-41	0.599329628278376
1102	42-43	0.6769571540195614
1102	44-45	0.6470149124395803
1102	46-47	0.6776689529986157
1102	48-49	0.6560101945209098
1102	50-51	0.613832465151944
1102	52-53	0.5836788246723614
1102	54-55	0.5908375729823092
1102	56-57	0.6327406097351016
1102	58-59	0.5937074699662332
1102	60-61	0.5897736953127009
1102	62-63	0.586044142050568
1102	64-65	0.6279450721569333
1102	66-67	0.6633994846671243
1102	68-69	0.5456455631682786
1102	70-71	0.5429688175254483
1102	72-73	0.5768156823835042
1102	74-75	0.5515913945511031
1102	76-77	0.543792737949012
1102	78-79	0.49559010458779085
1102	80-81	0.4951686081629205
1102	82-83	0.5446620759992413
1102	84-85	0.491598505654288
1102	86-87	0.5039014155579338
1102	88-89	0.5272592784008694
1102	90-91	0.5603600020947468
1102	92-93	0.5544874564852833
1102	94-95	0.5441587284844047
1102	96-97	0.5132496723453386
1102	98-99	0.5340649541591844
1102	100	0.5226940401554145
1103	1	0.3053854641941598
1103	2	-0.12988037106388362
1103	3	0.208634394472206
1103	4	0.20554723739731884
1103	5	0.22812658887072956
1103	6	0.28355966925030884
1103	7	0.3204213366848947
1103	8	0.30709817330014033
1103	9	0.3947373337797444
1103	10-11	0.35213759086216356
1103	12-13	0.4009803517975996
1103	14-15	0.5355297113260846
1103	16-17	0.5165306203588855
1103	18-19	0.525060144841845
1103	20-21	0.4812040628186196
1103	22-23	0.4748357032180479
1103	24-25	0.4778352147116962
1103	26-27	0.5315624735794131
1103	28-29	0.5137848758941104
1103	30-31	0.5405267282872472
1103	32-33	0.5397634880114239
1103	34-35	0.526616857038114
1103	36-37	0.5226250551394784
1103	38-39	0.5582505720945363
1103	40-41	0.6198159322739016
1103	42-43	0.6036816107133589
1103	44-45	0.6289916344995348
1103	46-47	0.6184070987954868
1103	48-49	0.6278363435909355
1103	50-51	0.6222965512439487
1103	52-53	0.6111485790360831
1103	54-55	0.5864955486106354
1103	56-57	0.579022427312772
1103	58-59	0.5835550999678887
1103	60-61	0.5990852527845334
1103	62-63	0.6031685740139991
1103	64-65	0.5818022283758779
1103	66-67	0.6305697723167967
1103	68-69	0.6219116592373695
1103	70-71	0.5447528279605365
1103	72-73	0.4823775535600916
1103	74-75	0.47771199026535527
1103	76-77	0.44820831619082924
1103	78-79	0.4440145294910707
1103	80-81	0.411043268170026
1103	82-83	0.4126366384739839
1103	84-85	0.36997161725751226
1103	86-87	0.4607351012592602
1103	88-89	0.5162091759096512
1103	90-91	0.4441193873846885
1103	92-93	0.39938130885109757
1103	94-95	0.4415610017640077
1103	96-97	0.45791298065681474
1103	98-99	0.46255322423628087
1103	100	0.4623837472924279
1104	1	0.39248391956022033
1104	2	0.5010208572248835
1104	3	0.3999141428443309
1104	4	0.30380544932295095
1104	5	0.3301060851049158
1104	6	0.33732718303227216
1104	7	0.36137693696571915
1104	8	0.3412897481850763
1104	9	0.3879028773153266
1104	10-11	0.3919302856671649
1104	12-13	0.46063758109750097
1104	14-15	0.5703980455308866
1104	16-17	0.5467144626260634
1104	18-19	0.5194775576748114
1104	20-21	0.5576653456181262
1104	22-23	0.5300269354617839
1104	24-25	0.5225176161812115
1104	26-27	0.5660436351185538
1104	28-29	0.5595165086805238
1104	30-31	0.5781832672834
1104	32-33	0.5798455051889491
1104	34-35	0.6362612488552699
1104	36-37	0.6641198164818931
1104	38-39	0.636300452090822
1104	40-41	0.5698732021146142
1104	42-43	0.6471835650103301
1104	44-45	0.6970133460326409
1104	46-47	0.6908892574694079
1104	48-49	0.7177226403610248
1104	50-51	0.6783909331042892
1104	52-53	0.6294079326585873
1104	54-55	0.6903487689424068
1104	56-57	0.6739820734818807
1104	58-59	0.6682642507079493
1104	60-61	0.7225023211263206
1104	62-63	0.6919265201383311
1104	64-65	0.6560266970804989
1104	66-67	0.6108946617182198
1104	68-69	0.6171025915214017
1104	70-71	0.5925822957117646
1104	72-73	0.6231261155704217
1104	74-75	0.584072219677708
1104	76-77	0.5566456014206622
1104	78-79	0.4952261090458663
1104	80-81	0.5174185946735363
1104	82-83	0.49631601563606154
1104	84-85	0.4770118434403443
1104	86-87	0.5398923215075158
1104	88-89	0.47575511619282906
1104	90-91	0.44930878947777586
1104	92-93	0.4976127622563169
1104	94-95	0.4818075012540106
1104	96-97	0.4818301756556025
1104	98-99	0.4199516445982354
1104	100	0.4741781102434146
1105	1	0.15833110483715984
1105	2	-0.3530893111513187
1105	3	0.07812738060249558
1105	4	0.16469999593083884
1105	5	0.21346008946278516
1105	6	0.2759115196992852
1105	7	0.28349693681381183
1105	8	0.28591523597084034
1105	9	0.3954013454856877
1105	10-11	0.380994744037217
1105	12-13	0.40463370591880476
1105	14-15	0.4479279543492112
1105	16-17	0.43414443028183314
1105	18-19	0.4800273624033622
1105	20-21	0.445261493382489
1105	22-23	0.43430431193228003
1105	24-25	0.45361494311719497
1105	26-27	0.4825670712936727
1105	28-29	0.5189396214395501
1105	30-31	0.5514012606212617
1105	32-33	0.5652480427814695
1105	34-35	0.570386713920584
1105	36-37	0.5826524736232557
1105	38-39	0.562233624115521
1105	40-41	0.5298214387786615
1105	42-43	0.5297181600029575
1105	44-45	0.5881674046838796
1105	46-47	0.599140780819809
1105	48-49	0.6051512002656878
1105	50-51	0.6222231040280661
1105	52-53	0.5910763102339303
1105	54-55	0.545032219407183
1105	56-57	0.5211316983063838
1105	58-59	0.5017363948490896
1105	60-61	0.5366900101005427
1105	62-63	0.5709980146448927
1105	64-65	0.5713617948825345
1105	66-67	0.5422129073643731
1105	68-69	0.6125786359843346
1105	70-71	0.544135654127949
1105	72-73	0.5229114564419675
1105	74-75	0.47779656996127784
1105	76-77	0.49011626578622014
1105	78-79	0.4531661068956083
1105	80-81	0.4494119715646505
1105	82-83	0.4247890980596907
1105	84-85	0.46546494496487156
1105	86-87	0.4417312111362932
1105	88-89	0.4544223504123579
1105	90-91	0.3819086451931071
1105	92-93	0.43182766862515365
1105	94-95	0.334899963787862
1105	96-97	0.38772751353931056
1105	98-99	0.3719280485942491
1105	100	0.4353711917556069
1106	1	0.29618074147611395
1106	2	0.338381979340344
1106	3	0.29212828760103804
1106	4	0.20973879373417503
1106	5	0.17693966176330633
1106	6	0.22728221482259414
1106	7	0.27587345299477306
1106	8	0.2620596314384471
1106	9	0.25941604963458786
1106	10-11	0.33117025001505596
1106	12-13	0.3386274348805216
1106	14-15	0.3897620374923676
1106	16-17	0.3519785429274833
1106	18-19	0.4387963818664815
1106	20-21	0.40301328596320474
1106	22-23	0.3794735973508594
1106	24-25	0.35112410749708545
1106	26-27	0.3691710059242794
1106	28-29	0.35736044251007115
1106	30-31	0.348203644151738
1106	32-33	0.35823239144026076
1106	34-35	0.3053679169102921
1106	36-37	0.3343289390409936
1106	38-39	0.4325892657966932
1106	40-41	0.3615587957101951
1106	42-43	0.3151969333002853
1106	44-45	0.31597107833924554
1106	46-47	0.32236761016699234
1106	48-49	0.2974745442997673
1106	50-51	0.41086084282736834
1106	52-53	0.3517925142866858
1106	54-55	0.379647794529113
1106	56-57	0.3456690607053545
1106	58-59	0.39235388600882715
1106	60-61	0.39254264083615453
1106	62-63	0.32152340931849466
1106	64-65	0.370169840184289
1106	66-67	0.4022798942548462
1106	68-69	0.34455978768301776
1106	70-71	0.333050384761421
1106	72-73	0.28792656564110075
1106	74-75	0.30032799992392967
1106	76-77	0.22962139806902115
1106	78-79	0.22641247825859523
1106	80-81	0.236702881130455
1106	82-83	0.20350182054804122
1106	84-85	0.2261391959365895
1106	86-87	0.266369565427901
1106	88-89	0.24015263919707053
1106	90-91	0.24885576820643962
1106	92-93	0.25927163521047447
1106	94-95	0.28797253300943737
1106	96-97	0.21167567198175163
1106	98-99	0.26959220254203586
1106	100	0.2843996463651486
1107	1	0.13721769144196827
1107	2	-0.37867832601476437
1107	3	0.037366562984118445
1107	4	0.10217248141966451
1107	5	0.14734865835912103
1107	6	0.2173190824393032
1107	7	0.16252409850203264
1107	8	0.20922756615661342
1107	9	0.17883301765594695
1107	10-11	0.26970500504862827
1107	12-13	0.22017960905830591
1107	14-15	0.2790557632535382
1107	16-17	0.32460723925127155
1107	18-19	0.3005772010356864
1107	20-21	0.2529052427643208
1107	22-23	0.2627590748205648
1107	24-25	0.2520156868297576
1107	26-27	0.2809489756178323
1107	28-29	0.21419630672119894
1107	30-31	0.3250989948246428
1107	32-33	0.38135940462267115
1107	34-35	0.4373554895736689
1107	36-37	0.43411151499206113
1107	38-39	0.4437551346798472
1107	40-41	0.3880649456310721
1107	42-43	0.379605359164934
1107	44-45	0.3682721239783717
1107	46-47	0.41605634905316435
1107	48-49	0.4018543904113514
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2216	88-89	-0.9080873374643907
2216	90-91	-0.9169106833309186
2216	92-93	-0.8481880444099126
2216	94-95	-0.7992670000908504
2216	96-97	-0.8977046621308915
2216	98-99	-0.9560080182556305
2216	100	-0.9666482897505411
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	9.0
13	73.0
14	256.0
15	765.0
16	1835.0
17	3741.0
18	6352.0
19	9968.0
20	13999.0
21	20201.0
22	27456.0
23	31549.0
24	33571.0
25	34803.0
26	38617.0
27	47732.0
28	59806.0
29	75356.0
30	93222.0
31	114731.0
32	143580.0
33	180652.0
34	247661.0
35	341488.0
36	513912.0
37	870734.0
38	752512.0
39	285416.0
40	535.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.188234007438524	21.58777127056689	13.573344857451069	24.650649864543514
2	14.924677941559905	20.530431326685854	39.97101537324352	24.573875358510723
3	17.910822930523192	26.808709376514855	29.952423201884336	25.328044491077613
4	12.24675510794194	17.345151386095946	38.87411322826184	31.53398027770027
5	12.550007543532256	38.77071813414526	35.309300010834335	13.369974311488143
6	29.508441137324727	39.086133672055475	17.80321343899331	13.60221175162649
7	25.834814573683758	32.44741575661723	24.16943803318957	17.548331636509438
8	26.10950823357045	35.04549005273717	19.8620490293211	18.982952684371277
9	26.210456611688443	14.226000839380196	21.59406804244692	37.96947450648444
10-11	25.495667168033485	25.91844545572826	27.65298311570031	20.932904260537942
12-13	25.843923884720393	23.780557261372767	28.067775141284695	22.30774371262214
14-15	23.18681093347368	24.733208427664454	25.717378520123507	26.362602118738355
16-17	21.73179767819007	27.15565310381114	27.12647891313546	23.98607030486333
18-19	21.253962122588995	27.08122496857387	29.597517702670068	22.067295206167064
20-21	22.748176701264537	26.345097318538365	28.721967066714054	22.184758913483044
22-23	23.15055536591728	25.966158490936664	28.461422702194795	22.421863440951263
24-25	22.62743753470259	26.045659402711053	29.28909475137978	22.037808311206575
26-27	22.538180387761315	26.227628594998688	28.362611735205697	22.8715792820343
28-29	21.804457321465907	26.833714125735913	28.80046089971634	22.561367653081838
30-31	22.657696402562287	26.38471153520005	28.621067430377767	22.336524631859895
32-33	23.105357402315736	26.64838475001101	27.789407710451297	22.456850137221956
34-35	22.937873028943446	26.429911850722227	28.680757911406225	21.9514572089281
36-37	22.757847420120925	26.027051026519455	28.420043973802024	22.7950575795576
38-39	22.960123871861374	25.96852729276599	28.964641235843054	22.10670759952958
40-41	22.40401173620918	25.38813352912245	29.481150403656848	22.726704331011526
42-43	22.618690219501662	25.624694025607937	28.536102067235134	23.220513687655263
44-45	22.652823643588434	26.526755623416985	28.298073121253985	22.52234761174059
46-47	22.86208649261087	26.273435439360853	27.613355561551934	23.251122506476342
48-49	23.629600163118656	26.08741656274518	27.47660833183458	22.806374942301584
50-51	23.015040498322506	26.13675771427356	27.230166858455085	23.61803492894885
52-53	22.39988315503676	26.676874569362013	27.148772798816566	23.774469476784656
54-55	22.569384061960594	26.734756592248445	26.891419869480178	23.804439476310787
56-57	23.570730111977355	26.12623467579422	26.755365291400068	23.547669920828366
58-59	22.469567656252316	26.389729670141353	27.74043605084944	23.400266622756895
60-61	22.65897926126893	27.086206892187402	26.913242802043573	23.341571044500096
62-63	23.08627137495842	26.24238900361318	26.519174369844684	24.15216525158371
64-65	22.181975905029546	26.468345798314864	26.97578352698648	24.373894769669114
66-67	22.853467404659725	26.070716009531875	26.962354962645556	24.113461623162845
68-69	22.329887452504018	25.9804825532586	27.35491551565252	24.334714478584864
70-71	22.343879592490488	25.70630260668027	27.41668775057844	24.533130050250797
72-73	22.97952631213907	25.811421949538975	27.69469899512319	23.514352743198767
74-75	22.99217964008043	26.31018461739798	27.193713538832785	23.503922203688806
76-77	22.786531786237514	26.19888429372882	26.79392341938975	24.22066050064392
78-79	22.474202091170923	26.361367153742528	26.91076401703677	24.253666738049784
80-81	22.09416856690388	26.800989473428405	27.461937877431662	23.642904082236054
82-83	22.932254267893917	25.69984651137065	28.39316883837826	22.974730382357173
84-85	23.274160861921857	26.288586264015905	26.86802495564564	23.56922791841659
86-87	21.849488633486914	26.929497240646043	27.42919345855906	23.791820667307984
88-89	22.79365424937076	25.848814079784514	27.124267679948055	24.23326399089667
90-91	22.364904470441825	25.825832895704963	27.494465883909946	24.314796749943262
92-93	22.749239049521684	26.209758418554596	26.97396825975464	24.06703427216908
94-95	21.57388446894116	26.148210784993097	27.678082674644465	24.599822071421276
96-97	22.031786920376764	26.016638333630144	27.194120600642652	24.757454145350437
98-99	21.368661345408263	26.78967224949904	27.104940609207475	24.736725795885224
100	22.141773689985676	26.077662813903192	26.33874473434123	25.44181876176991
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	147.0
2	128.0
3	162.5
4	113.0
5	85.5
6	180.0
7	478.5
8	785.5
9	875.0
10	698.0
11	489.5
12	509.5
13	624.5
14	774.0
15	1095.5
16	1437.0
17	1782.5
18	2348.5
19	3095.0
20	3952.5
21	4793.5
22	5770.0
23	6859.5
24	7782.5
25	8825.0
26	10835.0
27	13148.0
28	16511.5
29	24192.0
30	34827.5
31	44529.5
32	58355.0
33	70624.5
34	78470.5
35	89187.5
36	99338.0
37	117741.0
38	126050.0
39	117136.0
40	108796.5
41	100028.0
42	97488.0
43	103663.5
44	113901.0
45	135816.5
46	158964.0
47	174367.0
48	183940.5
49	179368.0
50	170445.5
51	173112.5
52	174267.5
53	169178.5
54	164205.0
55	143792.0
56	126213.5
57	107049.5
58	86698.5
59	83146.5
60	68960.0
61	47903.5
62	35505.5
63	29024.0
64	21566.5
65	11647.5
66	7002.0
67	5076.5
68	3732.5
69	2793.5
70	2025.5
71	1425.0
72	1109.5
73	1002.5
74	815.0
75	631.5
76	463.0
77	253.0
78	132.0
79	74.5
80	47.0
81	30.0
82	10.5
83	4.0
84	5.0
85	6.0
86	3.5
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014099359732127354
2	0.004151337515383996
3	0.0019997296568008277
4	0.027743084858907682
5	0.0032906943719507283
6	0.0
7	4.050085380862436E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008935500871527748
16-17	0.003151472686983582
18-19	0.0
20-21	5.062606726078045E-5
22-23	7.720475257269017E-4
24-25	1.2656516815195112E-5
26-27	2.784433699342924E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.936041557926093E-4
36-37	0.0
38-39	0.0
40-41	6.328258407597555E-4
42-43	0.0010125213452156089
44-45	0.0
46-47	0.0
48-49	8.986126938788528E-4
50-51	0.0
52-53	0.0
54-55	5.821997734989751E-4
56-57	7.593910089117067E-5
58-59	2.151607858583169E-4
60-61	7.593910089117067E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0017339428036817301
70-71	0.001252995164704316
72-73	0.0
74-75	3.1641292037987774E-4
76-77	0.003075533586092412
78-79	5.062606726078044E-4
80-81	0.0037969550445585334
82-83	0.0019870731399856323
84-85	0.0062143497562608
86-87	0.008454553232550333
88-89	0.008391270648474359
90-91	0.006695297395238213
92-93	0.009505044128211527
94-95	0.001835194938203291
96-97	0.005024637175632459
98-99	0.00227817302673512
100	0.0885196786054746
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3950534.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.152232868592428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.22268422653322	15.332030063959598
2	15.737513263888445	7.287171437162147
3	6.516504621384281	4.526148974506428
4	3.3749108961014884	3.1254689190916647
5	1.9347321085418405	2.2396684157651765
6	1.2590857302906444	1.7490387617526453
7	0.828574668760156	1.3428347576106376
8	0.5832167936931842	1.0802216816366745
9	0.4377477763278601	0.9121354609726016
>10	2.3996556283071238	10.387170773170766
>50	0.2792497555123114	4.571721919240962
>100	0.3195955260695445	16.388539127238534
>500	0.06380814223646877	10.520431888159012
>1k	0.04075417303801806	16.855877563301554
>5k	0.0013111262103383825	1.8775382267935212
>10k+	6.555631051691912E-4	1.8040020296378425
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	14816	0.37503790626786154	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12073	0.3056042550197011	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	11412	0.2888723397900132	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	11080	0.28046841262472366	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	11039	0.27943057824587764	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10895	0.2757855014031015	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9390	0.23768938578936416	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7554	0.19121465604396773	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6972	0.17648247047108062	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6537	0.16547130084186087	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6034	0.1527388449257746	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5880	0.14884063774669448	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5578	0.14119610159031665	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5468	0.13841166789097373	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5383	0.13626006003239058	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5356	0.13557660812437	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5035	0.12745112432901476	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5035	0.12745112432901476	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4994	0.12641328995016876	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4902	0.12408449085617285	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4898	0.12398323872165129	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4876	0.12342635198178273	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4847	0.12269227400650139	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4653	0.1177815454822057	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4530	0.1146680423456677	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4520	0.11441491200936381	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4515	0.11428834684121185	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4450	0.1126429996552365	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4410	0.11163047831002086	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4363	0.11044076572939254	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4150	0.10504908956611941	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4115	0.10416313338905576	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	4079	0.10325186417836171	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4044	0.10236590800129805	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4040	0.1022646558667765	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	3985	0.10087243901710503	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	3974	0.10059399564717074	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	3957	0.1001636740754541	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.531303363039022E-5
6	0.0	0.0	0.0	0.0	2.531303363039022E-5
7	0.0	0.0	0.0	0.0	2.531303363039022E-5
8	0.0	0.0	0.0	0.0	2.531303363039022E-5
9	0.0	0.0	0.0	0.0	2.531303363039022E-5
10-11	0.0	0.0	0.0	0.0	2.531303363039022E-5
12-13	0.0	0.0	0.0	0.0	1.0125213452156088E-4
14-15	0.0	0.0	0.0	0.0	1.265651681519511E-4
16-17	0.0	0.0	0.0	0.0	1.6453471859753644E-4
18-19	0.0	0.0	0.0	0.0	2.0250426904312176E-4
20-21	0.0	0.0	0.0	2.531303363039022E-5	2.531303363039022E-4
22-23	0.0	0.0	0.0	1.265651681519511E-4	2.784433699342924E-4
24-25	0.0	0.0	0.0	2.0250426904312176E-4	2.784433699342924E-4
26-27	0.0	0.0	0.0	5.315737062381947E-4	2.9109988674948754E-4
28-29	0.0	0.0	0.0	0.0014554994337474376	3.0375640356468267E-4
30-31	0.0	0.0	0.0	0.0068092060465749695	3.0375640356468267E-4
32-33	0.0	0.0	0.0	0.019731509714889177	3.5438247082546307E-4
34-35	0.0	0.0	0.0	0.03979208886697343	3.5438247082546307E-4
36-37	0.0	0.0	0.0	0.0748126708946183	3.5438247082546307E-4
38-39	0.0	0.0	0.0	0.13010899286020572	3.5438247082546307E-4
40-41	0.0	0.0	0.0	0.1933662639025509	3.5438247082546307E-4
42-43	0.0	0.0	0.0	0.2603572074053786	3.670389876406582E-4
44-45	0.0	0.0	0.0	0.34216893209879984	3.923520212710484E-4
46-47	0.0	0.0	0.0	0.44581314829843255	4.050085380862435E-4
48-49	0.0	0.0	0.0	0.5697710739864534	4.050085380862435E-4
50-51	0.0	0.0	0.0	0.7043731303160534	4.303215717166337E-4
52-53	0.0	0.0	0.0	0.8366590440684727	4.55634605347024E-4
54-55	0.0	0.0	0.0	0.9851452993443419	4.6829112216221905E-4
56-57	0.0	0.0	0.0	1.1677788369876072	4.809476389774142E-4
58-59	0.0	0.0	0.0	1.3917232455156694	4.809476389774142E-4
60-61	0.0	0.0	0.0	1.6267041367065818	5.062606726078044E-4
62-63	0.0	0.0	0.0	1.8696712900078825	5.062606726078044E-4
64-65	0.0	0.0	0.0	2.1271934376466577	5.062606726078044E-4
66-67	0.0	0.0	0.0	2.426279586506533	5.062606726078044E-4
68-69	0.0	0.0	0.0	2.768423205571702	5.062606726078044E-4
70-71	0.0	0.0	0.0	3.1448406721724202	5.062606726078044E-4
72-73	0.0	0.0	0.0	3.5378002062505978	5.189171894229995E-4
74-75	0.0	0.0	0.0	3.92163439170502	5.315737062381946E-4
76-77	0.0	0.0	0.0	4.365726253716586	5.315737062381946E-4
78-79	0.0	0.0	0.0	4.850774604142123	5.315737062381946E-4
80-81	0.0	0.0	0.0	5.379285433311042	5.315737062381946E-4
82-83	0.0	0.0	0.0	5.935387469137083	5.821997734989751E-4
84-85	0.0	0.0	0.0	6.467024457959354	5.821997734989751E-4
86-87	0.0	0.0	0.0	7.04050389137266	6.201693239445605E-4
88	0.0	0.0	0.0	7.494379240882372	6.581388743901457E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8680	0.0	39.16385	1
GGGCACC	3825	0.0	38.983074	94
GTATCAA	14205	0.0	35.482998	1
TATTCTC	2245	0.0	33.286346	5
GTACTAG	3970	0.0	30.911266	1
CTGTGCG	5210	0.0	30.313135	9
CAACGCA	16050	0.0	30.248972	5
ATCAACG	16160	0.0	30.051056	3
TAGTACT	5290	0.0	29.859627	4
CAGTACG	590	0.0	28.680042	9
TAGGCAT	4945	0.0	28.417786	5
TATCAAC	18205	0.0	27.47728	2
ACTGTGC	5970	0.0	27.47353	8
AACGCAG	18440	0.0	27.397844	6
TACTGTG	6605	0.0	26.540241	7
AGGGCTA	4905	0.0	26.349903	5
GTACTGT	6045	0.0	26.20007	6
GCTATTG	4930	0.0	26.11963	8
CTATTGA	5005	0.0	26.013874	9
CTAGTAC	6325	0.0	25.791094	3
>>END_MODULE