FastQCFastQC Report
Mon 16 Jan 2017
ERR1146424_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146424_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219451
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC106120.32962141681920304No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98020.30446184768769585No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT90730.2818182354693393No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA79220.2460667983454322No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC73350.22783387602420413No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA70100.2177389871751426No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC66770.20739560875441188No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC59100.18357167107062664No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG48890.15185818948634408No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT43460.13499195980929668No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT43330.1345881642553342No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC42580.13225857452093542No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT39340.12219474686833251No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC38830.12061062584894132No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT37730.11719389423848973No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT37340.11598250757660233No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA36850.11446050895012845No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC35160.10921116674861646No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC35130.1091179831592405No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC34610.10750280094339065No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG33460.10393076335064581No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG32630.10135268404457778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG31200.037.031481
GGGCACC30200.032.82578394
GCATCGT34000.032.5898178
CAACGCA60900.032.551275
TAGGCAT39400.032.5491185
GTATCAA70550.031.4166871
ATCAACG65850.030.2503473
CTAGGCA42850.030.039984
TCTAACG15000.029.7544982
CTAACGC14900.029.323583
GGTATCA46700.028.072911
TAACGCC16200.027.839164
CGCCTAC15850.026.9563437
AACGCAG78650.026.6802436
GTACATG128000.026.4897861
TATCAAC82850.026.4249212
TACATGG128250.026.0088562
ACGCAGA75500.025.8699267
CTGTGCG42200.025.5920419
TAGTACT42350.025.5137544
Produced by FastQC (version 0.11.5)