##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146424_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3219451
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.27935166585856	31.0	30.0	34.0	26.0	34.0
2	30.336800591156692	31.0	30.0	34.0	26.0	34.0
3	30.5843191898246	31.0	30.0	34.0	26.0	34.0
4	34.06022828115725	35.0	35.0	37.0	28.0	37.0
5	34.2536180858165	37.0	35.0	37.0	30.0	37.0
6	34.47750750050241	37.0	35.0	37.0	30.0	37.0
7	34.2979212915494	37.0	35.0	37.0	30.0	37.0
8	34.27952902529034	37.0	35.0	37.0	30.0	37.0
9	35.65557326388878	39.0	35.0	39.0	30.0	39.0
10-11	35.57042970991017	39.0	35.0	39.0	29.5	39.0
12-13	35.558826489361074	39.0	35.0	39.0	29.0	39.0
14-15	36.558395049342266	40.0	36.0	41.0	28.5	41.0
16-17	36.3873469420718	40.0	36.0	41.0	27.5	41.0
18-19	36.32993094164191	39.5	36.0	41.0	27.0	41.0
20-21	36.68184125181591	40.0	36.0	41.0	29.0	41.0
22-23	36.39129078218616	39.5	35.5	41.0	27.5	41.0
24-25	36.50252620710798	39.0	35.5	41.0	28.5	41.0
26-27	36.59167945093744	39.0	35.5	41.0	29.0	41.0
28-29	36.4329298690988	39.0	35.0	41.0	28.5	41.0
30-31	36.3766305186816	39.0	35.0	41.0	28.5	41.0
32-33	36.48660113168363	39.0	35.0	41.0	29.0	41.0
34-35	36.529414642434375	39.0	35.0	41.0	29.0	41.0
36-37	36.44577786709597	39.0	35.0	41.0	29.0	41.0
38-39	36.27135884347983	39.0	35.0	41.0	28.0	41.0
40-41	36.22825677421399	39.0	35.0	41.0	28.0	41.0
42-43	36.061092558948715	39.0	35.0	41.0	28.0	41.0
44-45	35.86467351110484	39.0	34.5	40.5	27.5	41.0
46-47	35.7376425359479	38.0	34.0	40.0	27.0	41.0
48-49	35.60170429678848	38.0	34.0	40.0	27.0	41.0
50-51	35.38639196558668	38.0	34.0	40.0	26.5	41.0
52-53	35.206760717898796	38.0	33.0	40.0	26.0	41.0
54-55	35.00910248362221	37.5	33.0	40.0	26.0	41.0
56-57	34.85792406842036	37.0	33.0	40.0	26.0	41.0
58-59	34.603749831881274	36.5	33.0	40.0	26.0	41.0
60-61	34.35081850911848	36.0	33.0	39.5	26.0	41.0
62-63	34.08376521338576	35.5	32.5	39.0	26.0	41.0
64-65	33.80049968146743	35.0	32.0	39.0	25.5	41.0
66-67	33.507326559714684	35.0	32.0	38.5	25.0	40.5
68-69	33.226288581500384	35.0	32.0	38.0	25.0	40.0
70-71	33.16203150785646	35.0	32.0	37.5	25.0	40.0
72-73	32.95969623392311	35.0	32.0	37.0	25.0	40.0
74-75	32.69554638352936	35.0	31.0	36.5	24.5	39.0
76-77	32.47020237301329	35.0	31.0	36.0	24.0	39.0
78-79	32.26131365254511	35.0	31.0	36.0	24.0	39.0
80-81	32.039294121886	35.0	31.0	35.0	24.0	37.5
82-83	31.818080008051062	35.0	31.0	35.0	24.0	37.0
84-85	31.63148685909492	34.0	31.0	35.0	23.0	37.0
86-87	31.46698753917982	34.0	31.0	35.0	23.0	36.0
88-89	31.480431135619085	35.0	31.0	35.0	23.0	36.0
90-91	31.408304086628434	35.0	31.0	35.0	23.0	36.0
92-93	31.267297436736882	34.0	31.0	35.0	23.0	36.0
94-95	31.245155462841335	34.0	31.0	35.0	23.0	35.0
96-97	31.156594866640305	34.0	31.0	35.0	23.0	35.0
98-99	31.080489499607232	34.0	31.0	35.0	22.5	35.0
100	30.580161338066645	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.669527911621735
1101	2	0.858627735458537
1101	3	0.8936337205396612
1101	4	0.9442299529110585
1101	5	0.9147780541746897
1101	6	0.6509471504452122
1101	7	0.6855552790122843
1101	8	0.7544347777644873
1101	9	0.9687971347134621
1101	10-11	1.0157421797908484
1101	12-13	0.8053451229395847
1101	14-15	1.0861386907691326
1101	16-17	1.1569486189357931
1101	18-19	1.2479838510326502
1101	20-21	0.8948224650293568
1101	22-23	1.2914108942694824
1101	24-25	1.1568235678950316
1101	26-27	1.0779329593411688
1101	28-29	1.2370941578071495
1101	30-31	1.244599519564133
1101	32-33	1.0586092182465308
1101	34-35	0.9485557027048515
1101	36-37	0.9555290640591068
1101	38-39	0.9918326408513991
1101	40-41	0.9407670544860167
1101	42-43	0.9717555019163129
1101	44-45	0.9014446128710745
1101	46-47	0.8990462167347744
1101	48-49	0.9678849072177726
1101	50-51	0.8263979738885894
1101	52-53	0.9238993568590317
1101	54-55	0.9305248568783071
1101	56-57	0.8040415121429163
1101	58-59	0.7104492076639488
1101	60-61	0.7596456571957901
1101	62-63	0.7339492348360892
1101	64-65	0.700018620560833
1101	66-67	0.7091469995291746
1101	68-69	0.7153562003223541
1101	70-71	0.6484155833897773
1101	72-73	0.5992621233938067
1101	74-75	0.6174724039712416
1101	76-77	0.5663441900012742
1101	78-79	0.6564761785980266
1101	80-81	0.7099345792413132
1101	82-83	0.6995098337530976
1101	84-85	0.6231144486020455
1101	86-87	0.6717956908340987
1101	88-89	0.6786623882901814
1101	90-91	0.65668979232602
1101	92-93	0.736555056612545
1101	94-95	0.6844949890632428
1101	96-97	0.5328183431565421
1101	98-99	0.6123199325710544
1101	100	0.6502949462737
1102	1	0.6401339834543016
1102	2	0.7938389088479845
1102	3	0.7631842514907454
1102	4	0.9378449051746287
1102	5	0.7749370910492459
1102	6	0.5086195658179449
1102	7	0.6106992620507867
1102	8	0.6257283002421019
1102	9	0.7572559910956187
1102	10-11	0.7745327126655184
1102	12-13	0.744912614911371
1102	14-15	0.837484022095559
1102	16-17	1.1022296632168036
1102	18-19	1.0878879828979606
1102	20-21	0.8886421585851494
1102	22-23	1.0665472237644096
1102	24-25	0.9579685228095371
1102	26-27	0.8136293745941998
1102	28-29	1.0151410543052393
1102	30-31	1.0754460446913185
1102	32-33	0.9329930439765093
1102	34-35	0.7773647569148991
1102	36-37	0.7169371848489092
1102	38-39	0.828609622032964
1102	40-41	0.7505022218745694
1102	42-43	0.7413889815965007
1102	44-45	0.7269112129749828
1102	46-47	0.6624408781700879
1102	48-49	0.6713977516882466
1102	50-51	0.7700698336292646
1102	52-53	0.6976760921510063
1102	54-55	0.8032730927425149
1102	56-57	0.6431500235474985
1102	58-59	0.5625060704920415
1102	60-61	0.7173207211026735
1102	62-63	0.6259456726776804
1102	64-65	0.4891770980425747
1102	66-67	0.5065337364862827
1102	68-69	0.6400140253125031
1102	70-71	0.567102644196197
1102	72-73	0.5125443341500784
1102	74-75	0.5030174606982882
1102	76-77	0.5654835164206276
1102	78-79	0.550135511812357
1102	80-81	0.5936599089658117
1102	82-83	0.5212919307664166
1102	84-85	0.5308097125828866
1102	86-87	0.5207652710190303
1102	88-89	0.47732152186423704
1102	90-91	0.4568788144480038
1102	92-93	0.5451175875037713
1102	94-95	0.47435726848775417
1102	96-97	0.4425338145728155
1102	98-99	0.4642196237007603
1102	100	0.46414760407772704
1103	1	0.49202325331781793
1103	2	0.7416525481956029
1103	3	0.6630154544305071
1103	4	0.7182105744588796
1103	5	0.7081137145169549
1103	6	0.4947913149508736
1103	7	0.47986086758754
1103	8	0.5537309867844442
1103	9	0.576879112571774
1103	10-11	0.6490855872765451
1103	12-13	0.5232792648509559
1103	14-15	0.6371059629263982
1103	16-17	0.9517315334906797
1103	18-19	0.984421609774536
1103	20-21	0.6327976962787432
1103	22-23	0.8902964785873309
1103	24-25	0.8187500349757713
1103	26-27	0.6635192040009059
1103	28-29	0.8934187423903808
1103	30-31	0.9241331395335806
1103	32-33	0.6899256983443536
1103	34-35	0.6721025701974526
1103	36-37	0.6912226226225471
1103	38-39	0.7751677346665318
1103	40-41	0.7363740625955586
1103	42-43	0.7425071874688811
1103	44-45	0.7361264100087652
1103	46-47	0.7112285575619453
1103	48-49	0.6998952909385778
1103	50-51	0.7340445702695533
1103	52-53	0.7773861893635967
1103	54-55	0.7102146210917084
1103	56-57	0.5919335990101047
1103	58-59	0.6467237520874676
1103	60-61	0.6061652230701924
1103	62-63	0.4963867700377307
1103	64-65	0.5351035501176398
1103	66-67	0.583112137537249
1103	68-69	0.5611760130659462
1103	70-71	0.5013974352533666
1103	72-73	0.556516864162738
1103	74-75	0.5768316433571314
1103	76-77	0.4901004230472026
1103	78-79	0.46656684059573905
1103	80-81	0.5444206059416317
1103	82-83	0.47236363820159255
1103	84-85	0.5209173289396176
1103	86-87	0.4678918467632478
1103	88-89	0.4547070723887323
1103	90-91	0.43753575551695434
1103	92-93	0.4633352357164888
1103	94-95	0.4846450381507452
1103	96-97	0.4981015526049255
1103	98-99	0.5927017043071352
1103	100	0.5513319058745587
1104	1	0.5171136997123824
1104	2	0.656197105952824
1104	3	0.5694436023932745
1104	4	0.6615742904511208
1104	5	0.5503471694781084
1104	6	0.3446254712512342
1104	7	0.4141895111528129
1104	8	0.38016995915789664
1104	9	0.5956314777540967
1104	10-11	0.6295084454312061
1104	12-13	0.5154986881686341
1104	14-15	0.7334309934953325
1104	16-17	0.7877350678247765
1104	18-19	0.7361025976265338
1104	20-21	0.6101610031665814
1104	22-23	0.9105332519410609
1104	24-25	0.688751527736116
1104	26-27	0.5885509346492341
1104	28-29	0.8189339117511025
1104	30-31	0.8417815530163537
1104	32-33	0.6453929976295854
1104	34-35	0.48286474963362025
1104	36-37	0.47827223571972155
1104	38-39	0.586406367364873
1104	40-41	0.5251932344977774
1104	42-43	0.4440398180254306
1104	44-45	0.6014052828486598
1104	46-47	0.4806391116053419
1104	48-49	0.36714724215705274
1104	50-51	0.47507335396903727
1104	52-53	0.5054693393035521
1104	54-55	0.47690684792567595
1104	56-57	0.4377311141943494
1104	58-59	0.42442299838776165
1104	60-61	0.41706787079879604
1104	62-63	0.38447921214505243
1104	64-65	0.4259725947935422
1104	66-67	0.48722346982054177
1104	68-69	0.4791322419311612
1104	70-71	0.3690682173625959
1104	72-73	0.36826418190170784
1104	74-75	0.37039237941706205
1104	76-77	0.3833399650689415
1104	78-79	0.3487110959133375
1104	80-81	0.3778242999691983
1104	82-83	0.37281174724022037
1104	84-85	0.3306183653374575
1104	86-87	0.2296220121847803
1104	88-89	0.2656095302953254
1104	90-91	0.32681876131366394
1104	92-93	0.34713230929878947
1104	94-95	0.3019563049530305
1104	96-97	0.3058123810853921
1104	98-99	0.4150908998809122
1104	100	0.49219809562732664
1105	1	0.44114827012559843
1105	2	0.5682393094472893
1105	3	0.534656176434968
1105	4	0.6085482591541549
1105	5	0.5194440739825765
1105	6	0.24974547592714202
1105	7	0.40037778751891295
1105	8	0.38899453689194985
1105	9	0.3979913934885033
1105	10-11	0.5117257761566094
1105	12-13	0.4214526744900411
1105	14-15	0.5669598152994553
1105	16-17	0.7127419320785933
1105	18-19	0.6678563722297071
1105	20-21	0.49352727277369723
1105	22-23	0.775724229358083
1105	24-25	0.626229434993725
1105	26-27	0.4393895170114419
1105	28-29	0.7114560339504834
1105	30-31	0.8118780765954341
1105	32-33	0.6735501590424207
1105	34-35	0.5134555235183598
1105	36-37	0.5576823114365865
1105	38-39	0.5804477997063557
1105	40-41	0.561322135649732
1105	42-43	0.5609846684939939
1105	44-45	0.5151845766622927
1105	46-47	0.4945014741303453
1105	48-49	0.551921831069258
1105	50-51	0.5978715214962023
1105	52-53	0.5783943036683468
1105	54-55	0.4742294594831762
1105	56-57	0.5002940978897499
1105	58-59	0.41368815424159067
1105	60-61	0.4971698757035412
1105	62-63	0.47552369421521234
1105	64-65	0.3959192559367679
1105	66-67	0.4954408847309253
1105	68-69	0.4510435486401363
1105	70-71	0.40633284051001795
1105	72-73	0.4057520192656412
1105	74-75	0.41879157031106473
1105	76-77	0.4213039785496946
1105	78-79	0.46591009043774534
1105	80-81	0.5645862667162831
1105	82-83	0.5203977973090375
1105	84-85	0.5073965249019423
1105	86-87	0.5279599635472643
1105	88-89	0.43685872914271684
1105	90-91	0.4542163471117995
1105	92-93	0.5662339618049117
1105	94-95	0.44687729200405
1105	96-97	0.4385376317101155
1105	98-99	0.47952885973940695
1105	100	0.532920291097092
1106	1	0.4925404545406735
1106	2	0.509798513303501
1106	3	0.5516982069929455
1106	4	0.7244501463493336
1106	5	0.490572300090264
1106	6	0.321066868289428
1106	7	0.38198252596184545
1106	8	0.4052603450419241
1106	9	0.563376569846092
1106	10-11	0.5244982277316481
1106	12-13	0.489506604516194
1106	14-15	0.6230758573477999
1106	16-17	0.6522499450986388
1106	18-19	0.6727161221452178
1106	20-21	0.49582712514202143
1106	22-23	0.8350123957016322
1106	24-25	0.7093350361036315
1106	26-27	0.5136183638836087
1106	28-29	0.7540458135702011
1106	30-31	0.7739997037454316
1106	32-33	0.6268444796699413
1106	34-35	0.5739617400566601
1106	36-37	0.45729277727674855
1106	38-39	0.5490147053110164
1106	40-41	0.5655362193551099
1106	42-43	0.5766677762295416
1106	44-45	0.5862558845365768
1106	46-47	0.56788615245339
1106	48-49	0.5556830590801454
1106	50-51	0.49113773874599076
1106	52-53	0.5488181666377017
1106	54-55	0.5167349289696617
1106	56-57	0.49010573411108993
1106	58-59	0.4610274046996068
1106	60-61	0.5006785290537081
1106	62-63	0.3613474967089374
1106	64-65	0.4928392680318865
1106	66-67	0.44538663453971594
1106	68-69	0.4941004135126903
1106	70-71	0.5395499657834932
1106	72-73	0.41354483689606525
1106	74-75	0.507578322592245
1106	76-77	0.3847162266420412
1106	78-79	0.44033779538784756
1106	80-81	0.5413593586214418
1106	82-83	0.5338956006209727
1106	84-85	0.5073399624074924
1106	86-87	0.5646551486204565
1106	88-89	0.5232310811108469
1106	90-91	0.4980387622235902
1106	92-93	0.5467803768455575
1106	94-95	0.5361215245861821
1106	96-97	0.505836683665791
1106	98-99	0.5305677953684445
1106	100	0.5590173011111439
1107	1	0.4340054666810005
1107	2	0.623831092625224
1107	3	0.5560037221971683
1107	4	0.7003685389681635
1107	5	0.5869427758062713
1107	6	0.3106240726136491
1107	7	0.31916564628777166
1107	8	0.40888371581388583
1107	9	0.473594314631562
1107	10-11	0.47347755440788575
1107	12-13	0.34238621000557856
1107	14-15	0.5952818914093001
1107	16-17	0.7504233617361749
1107	18-19	0.7460588984841081
1107	20-21	0.42425387405872783
1107	22-23	0.7375582160286029
1107	24-25	0.4597217643308227
1107	26-27	0.4090389126130418
1107	28-29	0.5873151859405468
1107	30-31	0.5724993517759742
1107	32-33	0.46130241336632594
1107	34-35	0.3564186678538661
1107	36-37	0.36827308127548974
1107	38-39	0.3798197008959221
1107	40-41	0.25172935771291804
1107	42-43	0.37549914237588666
1107	44-45	0.3122713330904503
1107	46-47	0.3472170961411365
1107	48-49	0.34442297032097
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2216	100	-0.49624906942168323
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	13186.0
1	2253.0
2	341.0
3	345.0
4	310.0
5	181.0
6	182.0
7	243.0
8	398.0
9	786.0
10	1434.0
11	2810.0
12	4563.0
13	6361.0
14	8136.0
15	10054.0
16	12064.0
17	14037.0
18	16196.0
19	18181.0
20	20664.0
21	23708.0
22	27009.0
23	31210.0
24	34785.0
25	39247.0
26	44503.0
27	50977.0
28	60400.0
29	71909.0
30	85403.0
31	101463.0
32	124538.0
33	158915.0
34	218956.0
35	290798.0
36	429388.0
37	624863.0
38	503444.0
39	165042.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82692247964494	20.77895880110568	12.346115511757198	24.04800320749218
2	15.61565665311038	19.837449358923358	40.531331558068175	24.015562429898086
3	18.593687677156826	26.61926255320557	28.941831076732743	25.845218692904865
4	12.163101279707266	16.58289544184055	39.74204266980719	31.51196060864499
5	13.145261342639603	38.52234587115409	34.969292618093256	13.363100168113052
6	30.09316454179014	38.26625557073894	18.19630101061429	13.444278876856627
7	27.00147287175027	31.765173419878334	23.620923145314812	17.61243056305658
8	26.281571587031173	34.99307089849171	20.03067598622234	18.69468152825478
9	26.254402507868313	14.911200479592345	21.451235200079932	37.383161812459406
10-11	25.70529973696956	25.763383152873985	27.587218026094444	20.944099084062003
12-13	25.803141540117537	23.71525067633999	27.524676046143036	22.956931737399437
14-15	23.21393023484929	24.855706575239847	24.918549763451995	27.01181342645887
16-17	22.342123297242782	27.469705469823825	26.25533235100918	23.93283888192421
18-19	21.859297580857458	28.01733923361499	27.795063786308294	22.328299399219258
20-21	23.528192996143943	27.183960476704012	26.644109376056345	22.643737151095696
22-23	23.925615403956808	26.77361309652851	26.460056315359772	22.84071518415491
24-25	23.264558411479793	26.93816283712322	27.14141493311167	22.65586381828532
26-27	23.19273224748297	27.147908529319114	26.209640030957694	23.44971919224022
28-29	22.398324149761866	27.67157319207852	26.74717987056464	23.182922787594972
30-31	23.17327104566229	27.25595062280386	26.611540495832674	22.959237835701177
32-33	23.520645349234762	27.53019220159295	25.77726633925859	23.171896109913696
34-35	23.57593691565729	27.282223240929376	26.685951667480662	22.45588817593267
36-37	23.23573252918537	26.937853177927888	26.386963932808253	23.43945036007849
38-39	23.382839943236615	26.993105072228413	26.966309855143432	22.657745129391543
40-41	23.06610258527588	26.24775836362262	27.456945902779033	23.229193148322466
42-43	22.94175854736856	26.46387510897406	26.866276902714798	23.72808944094258
44-45	23.09531004401592	27.315122595697733	26.756525670487314	22.833041689799035
46-47	23.14819919097154	26.97823981105283	26.313663985677792	23.55989701229784
48-49	23.654261946829152	26.75303503658	26.503834260407473	23.088868756183377
50-51	23.302482894799915	26.602136457798377	26.5348695626512	23.56051108475051
52-53	22.83834567389655	27.108467313133872	26.342974029155506	23.710212983814067
54-55	22.74978432038475	27.037815175256767	26.389300729602173	23.82309977475631
56-57	23.7202333137703	26.593532457852437	26.104269253546132	23.581964974831134
58-59	22.855734485387675	26.851981533779295	26.934251054600324	23.358032926232706
60-61	22.801536105457135	27.48716672477205	26.28678183970752	23.424515330063297
62-63	23.22951053853017	26.811900448144154	25.990315839760946	23.968273173564725
64-65	22.473221313278664	26.99957390180568	26.333727163189984	24.193477621725666
66-67	23.138983696551637	26.588235661490323	26.290270704067897	23.98250993789014
68-69	22.59980007736422	26.70386728959907	26.567207159485385	24.129125473551323
70-71	22.590581217802917	26.312713511950424	26.71697607666858	24.37972919357808
72-73	23.178492758628927	26.476868538561078	27.0311104684816	23.3135282343284
74-75	23.265009581605568	26.82862049706306	26.620718471302574	23.285651450028798
76-77	23.0496471063218	26.712412924087932	26.32649132648274	23.91144864310753
78-79	22.899429223958226	26.67786104848629	26.375825132761495	24.046884594793987
80-81	22.56167163347645	26.93001817841451	27.05164477726173	23.456665410847314
82-83	23.318621735402324	26.043072486106	27.89792665767775	22.740379120813927
84-85	23.50284705416125	26.623840493140666	26.580479265697832	23.29283318700025
86-87	22.256287993123568	27.25928891344757	27.027156136192914	23.457266957235944
88-89	23.271290545680458	26.034571676427298	26.80501577007662	23.889122007815626
90-91	22.96968369382674	26.034886560187047	27.022190188832123	23.973239557154084
92-93	23.276400831611276	26.262711278884986	26.901164507031638	23.559723382472097
94-95	22.189110820947967	26.27209364226025	27.612108230679176	23.92668730611261
96-97	22.54237524999933	26.18411790374431	27.102714807858717	24.170792038397636
98-99	21.983800887584742	26.858027265658663	26.968802639892576	24.189369206864022
100	22.70277195705073	26.203854230223193	26.205078911892322	24.888294900833756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11143.0
1	6606.0
2	1943.5
3	1478.0
4	847.5
5	503.5
6	420.5
7	391.0
8	397.0
9	385.0
10	341.5
11	325.0
12	327.5
13	346.5
14	351.5
15	424.5
16	499.0
17	560.5
18	714.5
19	904.5
20	1173.0
21	1495.0
22	2038.0
23	2892.5
24	3871.5
25	5042.5
26	6919.0
27	9329.0
28	12934.5
29	19374.0
30	28179.5
31	36633.5
32	47274.5
33	56756.0
34	63202.0
35	71493.5
36	79127.0
37	91871.5
38	98021.5
39	92358.5
40	86103.5
41	79927.0
42	79553.5
43	85191.0
44	93188.5
45	110869.5
46	128310.5
47	138939.0
48	146668.5
49	144901.5
50	138987.0
51	142727.0
52	145151.0
53	139934.5
54	133700.5
55	118093.0
56	104886.5
57	88754.5
58	72308.5
59	68111.0
60	56883.5
61	41376.0
62	32062.5
63	26125.5
64	19096.5
65	11187.5
66	7404.5
67	5640.5
68	4498.5
69	3445.5
70	2635.5
71	2096.0
72	1646.0
73	1384.0
74	1135.5
75	911.5
76	715.0
77	507.5
78	314.5
79	197.0
80	134.0
81	88.0
82	70.0
83	50.0
84	35.0
85	29.5
86	26.5
87	22.0
88	16.0
89	13.0
90	9.5
91	6.0
92	5.5
93	4.0
94	4.5
95	7.0
96	7.5
97	6.0
98	3.5
99	2.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9912870237813839
2	0.44405086457287285
3	0.47737952837300524
4	0.4625012152693115
5	0.43081879488148755
6	0.48632515295309664
7	0.5027254646832643
8	0.5061111350972572
9	0.5196848779496878
10-11	0.5330101312304489
12-13	0.5312862348269938
14-15	0.5291430122713469
16-17	0.5336779469543099
18-19	0.5317055609791856
20-21	0.5307271332907381
22-23	0.4933915751474397
24-25	0.5287858085120725
26-27	0.5084717860281147
28-29	0.513736658827856
30-31	0.5180075733409206
32-33	0.5193432047886425
34-35	0.5322336013189827
36-37	0.5503578094526054
38-39	0.5400610228265627
40-41	0.5450929366528641
42-43	0.541319001283138
44-45	0.551910869275538
46-47	0.5500782586844776
48-49	0.5222163654610678
50-51	0.536784066600175
52-53	0.5302612153438583
54-55	0.5393310847097843
56-57	0.5274967688590384
58-59	0.5333673349897234
60-61	0.5260679538219405
62-63	0.5217815087106465
64-65	0.5323112543101293
66-67	0.5299350727810426
68-69	0.5501714422738535
70-71	0.5342681096870243
72-73	0.5382750040301902
74-75	0.534920394812656
76-77	0.523614119301707
78-79	0.5542870508046247
80-81	0.5288634615032191
82-83	0.5276986666360196
84-85	0.536784066600175
86-87	0.8416652404400626
88-89	1.1049399416235874
90-91	1.0956371132842213
92-93	1.0955594602930747
94-95	1.100140986770726
96-97	1.0931211563710708
98-99	1.0895957105730139
100	1.085588816229848
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3219451.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.9335504444393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.08569153453448	26.579433433217087
2	12.206670838081358	8.528447029615624
3	4.461582381989242	4.675767396097284
4	2.2462775557298453	3.138818012212014
5	1.2401579963630263	2.1661560962511275
6	0.7643224028288169	1.6020297129021317
7	0.5072005555795723	1.2402821335650642
8	0.35223836591108004	0.9843949379217265
9	0.26841532148074093	0.8439030155707069
>10	1.43694283249611	9.50567163355996
>50	0.17248471932630416	4.252577439146284
>100	0.20368771325005433	15.33078128898494
>500	0.03642633280929996	8.711281423177184
>1k	0.01718895411294549	10.438647266661624
>5k	6.23433568863308E-4	1.6716433610335886
>10k+	8.9061938409044E-5	0.33016582008391077
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	10612	0.32962141681920304	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	9802	0.30446184768769585	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	9073	0.2818182354693393	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7922	0.2460667983454322	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	7335	0.22783387602420413	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	7010	0.2177389871751426	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6677	0.20739560875441188	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	5910	0.18357167107062664	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4889	0.15185818948634408	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	4346	0.13499195980929668	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	4333	0.1345881642553342	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	4258	0.13225857452093542	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	3934	0.12219474686833251	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	3883	0.12061062584894132	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	3773	0.11719389423848973	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	3734	0.11598250757660233	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	3685	0.11446050895012845	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	3516	0.10921116674861646	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	3513	0.1091179831592405	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	3461	0.10750280094339065	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	3346	0.10393076335064581	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	3263	0.10135268404457778	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.106119645865087E-5	3.106119645865087E-5
9	0.0	0.0	0.0	3.106119645865087E-5	3.106119645865087E-5
10-11	0.0	0.0	0.0	3.106119645865087E-5	3.106119645865087E-5
12-13	0.0	0.0	0.0	3.106119645865087E-5	7.765299114662717E-5
14-15	0.0	0.0	0.0	3.106119645865087E-5	9.31835893759526E-5
16-17	0.0	0.0	0.0	3.106119645865087E-5	1.0871418760527804E-4
18-19	0.0	0.0	0.0	3.106119645865087E-5	1.5530598229325434E-4
20-21	0.0	0.0	0.0	3.106119645865087E-5	3.106119645865087E-4
22-23	0.0	0.0	0.0	3.106119645865087E-5	3.4167316104515954E-4
24-25	0.0	0.0	0.0	7.765299114662717E-5	3.4167316104515954E-4
26-27	0.0	0.0	0.0	3.106119645865087E-4	3.4167316104515954E-4
28-29	0.0	0.0	0.0	0.001615182215849845	3.4167316104515954E-4
30-31	0.0	0.0	0.0	0.006507320658087358	3.4167316104515954E-4
32-33	0.0	0.0	0.0	0.01779806557080695	3.4167316104515954E-4
34-35	0.0	0.0	0.0	0.035176804989422106	3.727343575038104E-4
36-37	0.0	0.0	0.0	0.06616034845692635	3.727343575038104E-4
38-39	0.0	0.0	0.0	0.11640183372879412	4.1932615219178676E-4
40-41	0.0	0.0	0.0	0.17125590667477159	4.348567504211122E-4
42-43	0.0	0.0	0.0	0.22878124251619297	4.814485451090885E-4
44-45	0.0	0.0	0.0	0.3009519324878683	5.280403397970647E-4
46-47	0.0	0.0	0.0	0.3957507040796707	5.901627327143665E-4
48-49	0.0	0.0	0.0	0.5069342568034115	6.212239291730173E-4
50-51	0.0	0.0	0.0	0.628694146921323	6.212239291730173E-4
52-53	0.0	0.0	0.0	0.7467422240624255	6.833463220903191E-4
54-55	0.0	0.0	0.0	0.8798394508877445	6.833463220903191E-4
56-57	0.0	0.0	0.0	1.0466225452724705	6.988769203196445E-4
58-59	0.0	0.0	0.0	1.247324466190043	7.454687150076208E-4
60-61	0.0	0.0	0.0	1.4568011751071843	7.454687150076208E-4
62-63	0.0	1.5530598229325434E-5	0.0	1.6801156470466547	8.386523043835734E-4
64-65	0.0	3.106119645865087E-5	0.0	1.9125931719414273	8.697135008422244E-4
66-67	0.0	3.106119645865087E-5	0.0	2.1830585401051295	8.852440990715499E-4
68-69	0.0	3.106119645865087E-5	0.0	2.4886696520617955	9.007746973008753E-4
70-71	0.0	3.106119645865087E-5	0.0	2.8266931225230634	9.318358937595261E-4
72-73	0.0	4.65917946879763E-5	0.0	3.1805888643747027	9.318358937595261E-4
74-75	0.0	6.212239291730173E-5	0.0	3.530897038035367	9.784276884475024E-4
76-77	0.0	6.212239291730173E-5	0.0	3.9273932108300453	9.939582866768278E-4
78-79	0.0	6.212239291730173E-5	0.0	4.368664098319869	0.0010250194831354787
80-81	0.0	6.212239291730173E-5	0.0	4.850609622572296	0.0010405500813648041
82-83	0.0	6.212239291730173E-5	0.0	5.346827766597473	0.0010716112778234549
84-85	0.0	6.212239291730173E-5	0.0	5.829285800591467	0.0010871418760527805
86-87	0.0	6.212239291730173E-5	0.0	6.347852475468644	0.0011182030725114312
88	0.0	6.212239291730173E-5	0.0	6.759786062903271	0.0011492642689700822
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	3120	0.0	37.03148	1
GGGCACC	3020	0.0	32.825783	94
GCATCGT	3400	0.0	32.589817	8
CAACGCA	6090	0.0	32.55127	5
TAGGCAT	3940	0.0	32.549118	5
GTATCAA	7055	0.0	31.416687	1
ATCAACG	6585	0.0	30.250347	3
CTAGGCA	4285	0.0	30.03998	4
TCTAACG	1500	0.0	29.754498	2
CTAACGC	1490	0.0	29.32358	3
GGTATCA	4670	0.0	28.07291	1
TAACGCC	1620	0.0	27.83916	4
CGCCTAC	1585	0.0	26.956343	7
AACGCAG	7865	0.0	26.680243	6
GTACATG	12800	0.0	26.489786	1
TATCAAC	8285	0.0	26.424921	2
TACATGG	12825	0.0	26.008856	2
ACGCAGA	7550	0.0	25.869926	7
CTGTGCG	4220	0.0	25.592041	9
TAGTACT	4235	0.0	25.513754	4
>>END_MODULE