FastQCFastQC Report
Mon 16 Jan 2017
ERR1146424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219451
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC119040.36975248264377997No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT91490.2841788864001968No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA89810.27896060539514345No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA77240.23991668144661932No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC76670.23814619324847622No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC74400.23109530165236247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72730.22590808184376776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66090.20528344739522358No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC64930.2016803486060201No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG50000.15530598229325435No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC45410.1410488931187336No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT43740.1358616733101389No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT43670.13564424493492835No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG42920.13331465520052954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41210.12800319060610024No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT39980.12418266344168619No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA39140.1215735229391595No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC39140.1215735229391595No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG39120.1215114005462422No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC38430.1193681779905953No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT38010.11806360773933194No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT37260.11573401800493313No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT36070.11203773562635368No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA35520.1103293698211279No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC35370.10986345187424813No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA34750.10793765769381176No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT34610.10750280094339065No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA34290.10650884265671383No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT33670.10458304847627749No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66950.042.630971
GTATCAA110700.037.548431
GGGCACC32650.034.71972394
GTACTAG32950.031.8226661
CAACGCA131100.030.4356785
ATCAACG132550.030.1475543
TACCCCG2350.029.9986275
TATCAAC146750.028.1927682
TAGGCAT40650.028.0946545
TAGTACT43850.027.2318424
AACGCAG149250.027.1433966
CTGTGCG43750.026.9666889
TCAACGC157000.025.7819884
GGACCGT2208.913048E-1125.6347926
ACTGTGC51750.025.6100238
CTAGGCA47700.025.428124
TCTAACG14650.025.0316872
CTAACGC14150.024.918263
ACGCAGA161200.024.7230517
TCACGCA2301.5279511E-1024.5282364
Produced by FastQC (version 0.11.5)