##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146423_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3149878
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.43527336614307	31.0	30.0	34.0	27.0	34.0
2	30.572047234845286	31.0	31.0	34.0	26.0	34.0
3	30.80752333899916	31.0	31.0	34.0	27.0	34.0
4	34.27378584186435	37.0	35.0	37.0	30.0	37.0
5	34.52778964772604	37.0	35.0	37.0	30.0	37.0
6	34.64230995613163	37.0	35.0	37.0	31.0	37.0
7	34.506353579408476	37.0	35.0	37.0	30.0	37.0
8	34.47092554060824	37.0	35.0	37.0	30.0	37.0
9	35.840054122731104	39.0	35.0	39.0	30.0	39.0
10-11	35.756155159025205	39.0	35.0	39.0	30.0	39.0
12-13	35.73695314548691	39.0	35.0	39.0	30.0	39.0
14-15	36.764463893522226	40.0	36.0	41.0	29.5	41.0
16-17	36.619028736986	40.0	36.0	41.0	28.5	41.0
18-19	36.57295377789235	40.0	36.0	41.0	28.0	41.0
20-21	36.89705775906241	40.0	36.0	41.0	30.0	41.0
22-23	36.63497856107443	40.0	36.0	41.0	28.5	41.0
24-25	36.74888424250082	40.0	36.0	41.0	29.5	41.0
26-27	36.81410946709682	40.0	36.0	41.0	30.0	41.0
28-29	36.66428715651844	40.0	35.5	41.0	29.5	41.0
30-31	36.621115008263814	40.0	35.0	41.0	29.5	41.0
32-33	36.722131142856966	40.0	35.5	41.0	30.0	41.0
34-35	36.772758659224266	40.0	35.0	41.0	30.0	41.0
36-37	36.707328664792726	40.0	35.0	41.0	30.0	41.0
38-39	36.535068659802064	39.0	35.0	41.0	29.0	41.0
40-41	36.48876623158103	39.0	35.0	41.0	29.0	41.0
42-43	36.33392055184359	39.0	35.0	41.0	29.0	41.0
44-45	36.14243853253999	39.0	35.0	41.0	28.0	41.0
46-47	36.00635627792568	39.0	35.0	41.0	28.0	41.0
48-49	35.867181046377034	38.0	34.0	40.5	28.0	41.0
50-51	35.64408129457712	38.0	34.0	40.0	27.5	41.0
52-53	35.44702921827449	38.0	34.0	40.0	27.0	41.0
54-55	35.255795462554424	38.0	34.0	40.0	27.0	41.0
56-57	35.095210036706185	37.0	33.0	40.0	27.0	41.0
58-59	34.82314362651506	37.0	33.0	40.0	26.5	41.0
60-61	34.56839693473842	36.0	33.0	40.0	26.0	41.0
62-63	34.29673800064637	35.5	33.0	39.0	26.0	41.0
64-65	34.01011562987519	35.0	33.0	39.0	26.0	41.0
66-67	33.72356627780505	35.0	32.0	39.0	26.0	41.0
68-69	33.44433736798695	35.0	32.0	38.0	25.0	40.0
70-71	33.39391430398257	35.0	32.0	37.5	26.0	40.0
72-73	33.20475856525237	35.0	32.0	37.0	25.5	40.0
74-75	32.943967353656234	35.0	32.0	36.5	25.0	39.5
76-77	32.71781034059097	35.0	32.0	36.0	25.0	39.0
78-79	32.51109757266789	35.0	32.0	36.0	25.0	39.0
80-81	32.30403034657215	35.0	31.5	35.5	24.5	38.0
82-83	32.06512363335977	35.0	31.0	35.0	24.0	37.0
84-85	31.885697636543384	35.0	31.0	35.0	24.0	37.0
86-87	31.712816655121244	35.0	31.0	35.0	24.0	36.5
88-89	31.721229996844322	35.0	32.0	35.0	24.0	36.0
90-91	31.642708701733845	35.0	32.0	35.0	24.0	36.0
92-93	31.502096112928818	35.0	31.0	35.0	23.5	36.0
94-95	31.481718815776357	35.0	31.0	35.0	24.0	35.5
96-97	31.392751878009243	35.0	31.0	35.0	23.0	35.0
98-99	31.31912108976919	35.0	31.0	35.0	23.0	35.0
100	30.829956271322253	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6596151706110298
1101	2	0.8691709762792179
1101	3	0.8984146841259886
1101	4	0.8518863250340516
1101	5	0.8195586669177999
1101	6	0.5665855767333952
1101	7	0.6589535396304171
1101	8	0.6466189145120111
1101	9	0.825016008519043
1101	10-11	0.900761669214468
1101	12-13	0.7257141371272695
1101	14-15	1.0428001274851582
1101	16-17	1.1225105754161788
1101	18-19	1.2030246262673288
1101	20-21	0.8919643200737681
1101	22-23	1.1666345142672867
1101	24-25	0.9494019121343698
1101	26-27	0.8882312941578263
1101	28-29	1.1130126102551756
1101	30-31	1.112381434971418
1101	32-33	0.9389462120030245
1101	34-35	0.8570847706743123
1101	36-37	0.8806533937449004
1101	38-39	0.9759799795124593
1101	40-41	0.9062992536371723
1101	42-43	0.9483666851636343
1101	44-45	0.9285233171158254
1101	46-47	0.9167550451759254
1101	48-49	0.8619758482340103
1101	50-51	0.8784209980588145
1101	52-53	0.879504007516573
1101	54-55	0.8898387372175378
1101	56-57	0.7643883720260618
1101	58-59	0.7178868445861397
1101	60-61	0.761912952134459
1101	62-63	0.6810964437446785
1101	64-65	0.6724735422847843
1101	66-67	0.7414278384836734
1101	68-69	0.7699037473255217
1101	70-71	0.6683009806277838
1101	72-73	0.5914867362006788
1101	74-75	0.662227431092461
1101	76-77	0.6818985352652902
1101	78-79	0.6535994155472622
1101	80-81	0.6671035338902271
1101	82-83	0.6879844206208148
1101	84-85	0.682057979925947
1101	86-87	0.5641698252348419
1101	88-89	0.617376440514203
1101	90-91	0.6428794099854507
1101	92-93	0.7181044004345054
1101	94-95	0.6713150663668124
1101	96-97	0.6190718392980479
1101	98-99	0.6225403528550544
1101	100	0.6967507132519586
1102	1	0.6108134186559333
1102	2	0.8280494946575381
1102	3	0.7953897477746708
1102	4	0.8895802527287842
1102	5	0.7850416548683015
1102	6	0.5938200248437866
1102	7	0.6015681043916814
1102	8	0.6869557146199838
1102	9	0.8872006913923016
1102	10-11	0.8654553165212349
1102	12-13	0.7744436343773486
1102	14-15	1.0633261348635372
1102	16-17	1.2108527599508747
1102	18-19	1.1935841022389369
1102	20-21	0.9693822958986829
1102	22-23	1.206095588878057
1102	24-25	1.0798560444122671
1102	26-27	0.9935168251225974
1102	28-29	1.125687658925898
1102	30-31	1.1679437182598136
1102	32-33	1.0600686033007491
1102	34-35	0.8951580928547571
1102	36-37	0.8801735415416729
1102	38-39	0.9818212874051113
1102	40-41	0.8549062997846377
1102	42-43	0.9211946961283175
1102	44-45	0.9073897897335499
1102	46-47	0.9166361609810423
1102	48-49	0.8731984704030751
1102	50-51	0.9197053200939109
1102	52-53	0.8270177047860159
1102	54-55	0.9028154836523399
1102	56-57	0.7836057749002023
1102	58-59	0.7364807642075917
1102	60-61	0.8205223828399326
1102	62-63	0.7436426483753564
1102	64-65	0.7185437837873536
1102	66-67	0.7093794779360536
1102	68-69	0.849431583450631
1102	70-71	0.7695360472567856
1102	72-73	0.7002361775824468
1102	74-75	0.7489230141531777
1102	76-77	0.7076561346309802
1102	78-79	0.6747931297239873
1102	80-81	0.7688056210200926
1102	82-83	0.7089084590050163
1102	84-85	0.6216826816557024
1102	86-87	0.6050350356102214
1102	88-89	0.6919038462426279
1102	90-91	0.7135112483567134
1102	92-93	0.7780776386971411
1102	94-95	0.7371395609103963
1102	96-97	0.6972088105412091
1102	98-99	0.7090170522784618
1102	100	0.7277549703983581
1103	1	0.5938868590487232
1103	2	0.7643618637799996
1103	3	0.7429170981567346
1103	4	0.6951772616370917
1103	5	0.7591832095571647
1103	6	0.49691839100064783
1103	7	0.5828643362703971
1103	8	0.6438219332986392
1103	9	0.7167913206564975
1103	10-11	0.8339797819342323
1103	12-13	0.7334525396172182
1103	14-15	0.8721625332365264
1103	16-17	1.0905325798342105
1103	18-19	1.1873416035808333
1103	20-21	0.903794171497168
1103	22-23	1.089246125595949
1103	24-25	1.0006436018025653
1103	26-27	0.8492288642052159
1103	28-29	1.0377640226823317
1103	30-31	1.1308664906252304
1103	32-33	0.9570555115154207
1103	34-35	0.8898290013550891
1103	36-37	0.8462607187488018
1103	38-39	0.9577231569909941
1103	40-41	0.8762819086267513
1103	42-43	0.9154235293860182
1103	44-45	0.9422723829344761
1103	46-47	0.9611253863088791
1103	48-49	0.9568619203714803
1103	50-51	0.9071810067234622
1103	52-53	0.9775461678431299
1103	54-55	0.9665064548587026
1103	56-57	0.902434133447251
1103	58-59	0.8588073685060991
1103	60-61	0.9767208813045372
1103	62-63	0.8594538453111582
1103	64-65	0.9047149760236621
1103	66-67	0.8878789715114834
1103	68-69	0.9015166013463372
1103	70-71	0.842835147978839
1103	72-73	0.7905274926507104
1103	74-75	0.7513874089355141
1103	76-77	0.8218841308867368
1103	78-79	0.7563202666321658
1103	80-81	0.7826375003505177
1103	82-83	0.7168125984787181
1103	84-85	0.7315237127192198
1103	86-87	0.7868022922480691
1103	88-89	0.7735840239634761
1103	90-91	0.7795219435520941
1103	92-93	0.8345419428682561
1103	94-95	0.77768731340662
1103	96-97	0.7453324288161092
1103	98-99	0.7547003491753301
1103	100	0.8624333428058577
1104	1	0.4667336790574659
1104	2	0.6411228150999442
1104	3	0.6357438835742606
1104	4	0.6602395196678543
1104	5	0.6527087436590548
1104	6	0.28641615672826504
1104	7	0.32525388443035297
1104	8	0.39525466815882737
1104	9	0.5428027443742565
1104	10-11	0.5398285130767917
1104	12-13	0.3743060255129862
1104	14-15	0.5883338105263576
1104	16-17	0.7899281183677047
1104	18-19	0.7735068104818339
1104	20-21	0.5817184985995638
1104	22-23	0.8111187982288826
1104	24-25	0.6495633572636095
1104	26-27	0.5293183735885378
1104	28-29	0.7320574725096805
1104	30-31	0.7329147659885322
1104	32-33	0.6399304368857983
1104	34-35	0.5176556175095541
1104	36-37	0.4891591716844417
1104	38-39	0.6246465008288951
1104	40-41	0.546543235402936
1104	42-43	0.5174467230414237
1104	44-45	0.5887757543468695
1104	46-47	0.5707848828897681
1104	48-49	0.5539747542043969
1104	50-51	0.5865925406160812
1104	52-53	0.642938681184134
1104	54-55	0.5876021331111687
1104	56-57	0.516319128430915
1104	58-59	0.5274044162505831
1104	60-61	0.486878472906632
1104	62-63	0.432169371042221
1104	64-65	0.45039207442397355
1104	66-67	0.5071115251063247
1104	68-69	0.4115130468221011
1104	70-71	0.3499411189640682
1104	72-73	0.3140964888899873
1104	74-75	0.373881123120988
1104	76-77	0.4070937708795981
1104	78-79	0.3155172519032021
1104	80-81	0.38200449497718125
1104	82-83	0.37650110059296793
1104	84-85	0.3368660395631089
1104	86-87	0.4138832661569367
1104	88-89	0.35414773919530873
1104	90-91	0.4593344770200183
1104	92-93	0.41646845654162945
1104	94-95	0.44866416129760367
1104	96-97	0.47948725219780286
1104	98-99	0.5302674573621289
1104	100	0.6657652861304335
1105	1	0.5172341243689722
1105	2	0.6309919783384181
1105	3	0.6554489177148106
1105	4	0.708237738421829
1105	5	0.6763420614418223
1105	6	0.4105016777427224
1105	7	0.4534161262557035
1105	8	0.42806458376169587
1105	9	0.5256052381186862
1105	10-11	0.599508206872116
1105	12-13	0.5524253265859755
1105	14-15	0.7395608027652116
1105	16-17	0.882519778319363
1105	18-19	0.9037710286420619
1105	20-21	0.7332914024546895
1105	22-23	0.9562492638974902
1105	24-25	0.7559225646091292
1105	26-27	0.582694789042975
1105	28-29	0.8656535749737415
1105	30-31	0.9027918282049185
1105	32-33	0.7184762297523264
1105	34-35	0.6659151917069295
1105	36-37	0.6252959353300582
1105	38-39	0.7685019972451954
1105	40-41	0.6210878513747815
1105	42-43	0.6813703945287628
1105	44-45	0.7014302880421042
1105	46-47	0.5970569364119669
1105	48-49	0.6915282976116686
1105	50-51	0.7058909629410266
1105	52-53	0.6500119773374848
1105	54-55	0.6251395074697044
1105	56-57	0.6249393519348772
1105	58-59	0.5491438348009936
1105	60-61	0.5791414505562287
1105	62-63	0.5551959518980709
1105	64-65	0.4099031860063107
1105	66-67	0.5350110861564161
1105	68-69	0.5454855435352712
1105	70-71	0.4846478495294022
1105	72-73	0.4708050537146278
1105	74-75	0.5266561029759416
1105	76-77	0.5911664626816275
1105	78-79	0.5530591323900751
1105	80-81	0.6203269959314213
1105	82-83	0.5610522371975719
1105	84-85	0.5539845560396408
1105	86-87	0.6196264592524869
1105	88-89	0.5698441745827374
1105	90-91	0.6002093020762125
1105	92-93	0.714290406157815
1105	94-95	0.5640342999803316
1105	96-97	0.5676642317134721
1105	98-99	0.4951276083439353
1105	100	0.6587860885393546
1106	1	0.505607079597354
1106	2	0.596683598396929
1106	3	0.5115053714725057
1106	4	0.6389794729994591
1106	5	0.5198133205170166
1106	6	0.30220803246222516
1106	7	0.32972249764696215
1106	8	0.36741613165529685
1106	9	0.49698581869291303
1106	10-11	0.4800277306948715
1106	12-13	0.2619145752849832
1106	14-15	0.5633987611890134
1106	16-17	0.7170720621521198
1106	18-19	0.6396270044759476
1106	20-21	0.4866435775240632
1106	22-23	0.7781305530215405
1106	24-25	0.5876290781072981
1106	26-27	0.4744292872825113
1106	28-29	0.7623394273969097
1106	30-31	0.7009555131403928
1106	32-33	0.5971281789590392
1106	34-35	0.5472058241620914
1106	36-37	0.5131979133153948
1106	38-39	0.6732977880225377
1106	40-41	0.601174425447013
1106	42-43	0.5644526061569266
1106	44-45	0.5631823908671763
1106	46-47	0.6022811120529354
1106	48-49	0.6237785486804412
1106	50-51	0.6304193108886125
1106	52-53	0.6011260677540093
1106	54-55	0.6179321861850298
1106	56-57	0.501187034758594
1106	58-59	0.4804378809976484
1106	60-61	0.47281818908347617
1106	62-63	0.5282047879313012
1106	64-65	0.45836196110823124
1106	66-67	0.48081710624985874
1106	68-69	0.43775101750946277
1106	70-71	0.44456937316956413
1106	72-73	0.42338492931153127
1106	74-75	0.4341190976211351
1106	76-77	0.46010838144252375
1106	78-79	0.4452662948960864
1106	80-81	0.5490561314212385
1106	82-83	0.42732685285394467
1106	84-85	0.5315735614103296
1106	86-87	0.46517589300803763
1106	88-89	0.4916983919428013
1106	90-91	0.5218203697437112
1106	92-93	0.5285896761728566
1106	94-95	0.4772851941027376
1106	96-97	0.5411288816263422
1106	98-99	0.5203052419859588
1106	100	0.578999169520447
1107	1	0.3386328587936056
1107	2	0.5305446574716832
1107	3	0.3731031959673672
1107	4	0.4759775148906513
1107	5	0.5012942366652453
1107	6	0.2257493955834775
1107	7	0.2851857753977569
1107	8	0.24912851322440588
1107	9	0.4156043112574963
1107	10-11	0.38798279826504256
1107	12-13	0.3058572350751376
1107	14-15	0.4295980064691207
1107	16-17	0.5565815520416493
1107	18-19	0.6292780910721518
1107	20-21	0.38662663679924947
1107	22-23	0.6577809650723907
1107	24-25	0.38950643419786957
1107	26-27	0.27206687526249596
1107	28-29	0.5167179205782162
1107	30-31	0.49918089994397263
1107	32-33	0.47272339778155015
1107	34-35	0.4221227972650752
1107	36-37	0.3689976928354213
1107	38-39	0.3589333061119149
1107	40-41	0.29315080479590705
1107	42-43	0.35449695269592496
1107	44-45	0.3512782071441478
1107	46-47	0.2907222184615037
1107	48-49	0.36779199718758093
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2214	1	-0.7614715125533991
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2214	3	-1.0013315738156905
2214	4	-0.7186517980833926
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2214	16-17	-3.181117815980471
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2214	90-91	-0.886293282239059
2214	92-93	-0.9640755110874579
2214	94-95	-1.04064825359605
2214	96-97	-1.0111268982883566
2214	98-99	-1.155471315194145
2214	100	-1.008926146689543
2215	1	-4.064295218720439
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2215	3	-3.752904726250488
2215	4	-2.0866097579714094
2215	5	-1.5320154346150119
2215	6	-1.3952145995053726
2215	7	-1.2764439713573381
2215	8	-1.2612080210870928
2215	9	-1.8887233309966334
2215	10-11	-1.472005698219668
2215	12-13	-1.3609813115264018
2215	14-15	-3.7263555514201414
2215	16-17	-3.3536160692619177
2215	18-19	-2.2922474207847685
2215	20-21	-1.8462744197672691
2215	22-23	-3.3100352787747127
2215	24-25	-5.576377937574623
2215	26-27	-3.6330799005542715
2215	28-29	-1.7982977593402154
2215	30-31	-3.8881333326286267
2215	32-33	-4.135520720471845
2215	34-35	-3.037790206607177
2215	36-37	-1.9934054374612415
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2215	40-41	-2.0204836154685637
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2215	62-63	-2.73418804550273
2215	64-65	-1.9708020709482383
2215	66-67	-1.7188172130510537
2215	68-69	-1.8659148041639213
2215	70-71	-1.667062635985772
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2215	80-81	-1.7436529937603105
2215	82-83	-1.6012057891406215
2215	84-85	-1.4298528042980791
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2215	92-93	-3.987754518467561
2215	94-95	-2.127054256640971
2215	96-97	-1.820478459692339
2215	98-99	-3.241810075621764
2215	100	-3.1656322674263215
2216	1	-0.4268227594617109
2216	2	-0.32372568044434047
2216	3	-0.6634712816620727
2216	4	-0.5029812358038868
2216	5	-0.625201254559336
2216	6	-0.5191226051505637
2216	7	-0.6744873192686853
2216	8	-0.5479369595980543
2216	9	-0.6954755774121963
2216	10-11	-0.6910035428726218
2216	12-13	-0.8745052510977729
2216	14-15	-1.2236156748223905
2216	16-17	-0.9461176598784462
2216	18-19	-2.8553683689401765
2216	20-21	-1.200299507471371
2216	22-23	-0.6849370058002577
2216	24-25	-0.8141557670224842
2216	26-27	-0.8298960605135761
2216	28-29	-0.748186398568663
2216	30-31	-0.9491588945231584
2216	32-33	-0.9151328566469417
2216	34-35	-1.1628655803404087
2216	36-37	-1.1119497362195432
2216	38-39	-1.2244943954576186
2216	40-41	-1.2763302145084836
2216	42-43	-1.2760330514330818
2216	44-45	-1.0854282968089919
2216	46-47	-1.212624190065668
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2216	50-51	-1.0719099200287303
2216	52-53	-1.1491947978718144
2216	54-55	-1.0051855429913346
2216	56-57	-1.0469332885139195
2216	58-59	-1.104827866770627
2216	60-61	-1.162076950596564
2216	62-63	-1.1093346182613715
2216	64-65	-1.0563531429497957
2216	66-67	-0.9895414138851493
2216	68-69	-1.1293826934386928
2216	70-71	-1.2148582141070605
2216	72-73	-1.1595479504561155
2216	74-75	-1.0804591879219956
2216	76-77	-1.0328869170924833
2216	78-79	-1.2246688259567122
2216	80-81	-1.260874068671832
2216	82-83	-1.465020830912362
2216	84-85	-1.3265533407039989
2216	86-87	-1.2492071667417513
2216	88-89	-1.1493882401715787
2216	90-91	-1.159420838409769
2216	92-93	-1.2310592383182453
2216	94-95	-1.1792082297297846
2216	96-97	-1.2024223022977516
2216	98-99	-1.1946255197155544
2216	100	-1.179081520241695
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	12921.0
1	2131.0
2	347.0
3	323.0
4	295.0
5	202.0
6	167.0
7	217.0
8	417.0
9	700.0
10	1255.0
11	2300.0
12	3858.0
13	5347.0
14	7016.0
15	8808.0
16	10304.0
17	12517.0
18	14329.0
19	16082.0
20	18381.0
21	21176.0
22	24366.0
23	28419.0
24	31577.0
25	36467.0
26	41268.0
27	46641.0
28	55886.0
29	66429.0
30	79037.0
31	93866.0
32	116376.0
33	147851.0
34	207506.0
35	281779.0
36	416051.0
37	633604.0
38	509585.0
39	193917.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48481068270954	22.051014812510623	12.989439792708229	23.4747347120716
2	15.174814113709218	20.09073955254628	41.1970133185024	23.5374330152421
3	18.110655206889138	27.58382530368389	29.293795782272735	25.01172370715424
4	12.149622139412104	17.318623568689357	40.68196396282029	29.849790329078246
5	12.961134261864729	38.77193055177799	35.13071681811819	13.136218368239087
6	28.79124006183816	39.12246214390579	18.67742773159482	13.408870062661224
7	26.15657051512149	32.14955418036671	24.391000330236764	17.302874974275035
8	25.800494005847536	35.1102163995774	20.34817807822727	18.74111151634779
9	25.950164512000917	15.235311200538693	22.31740125290808	36.49712303455231
10-11	25.525029946312078	26.117668499671414	27.60467484832149	20.752626705695018
12-13	25.722651047972683	24.01319941777788	27.59240366421404	22.671745870035398
14-15	23.237806780345313	25.09951735379985	25.12865644989743	26.534019415957406
16-17	21.991773952701426	27.59867115487825	26.58895121806813	23.820603674352196
18-19	21.427533011351805	28.33867265202297	28.217869735518214	22.015924601107013
20-21	23.31370797375583	27.329821258901866	26.860012683554878	22.496458083787424
22-23	23.921476106774602	26.92560651690121	26.644407468077574	22.50850990824661
24-25	23.206775563913553	27.167315697917775	27.28259501008455	22.343313728084123
26-27	23.040308158808873	27.37195667410186	26.379413194484886	23.208321972604384
28-29	22.087993934357804	27.946645452236258	27.03175718539906	22.933603428006876
30-31	22.96690821275309	27.4409810339917	26.851984079179676	22.740126674075533
32-33	23.465196831497632	27.874175786652643	25.781020132154964	22.879607249694757
34-35	23.422002724360816	27.570764442367768	26.948703119993155	22.058529713278258
36-37	23.08215217328501	27.1871192782909	26.64591087426992	23.08481767415417
38-39	23.290092260000552	27.031434855349108	27.252201965598122	22.426270919052218
40-41	22.8749413657226	26.2595217358204	27.838860636437474	23.026676262019528
42-43	22.77448064112807	26.588783450739626	27.145241094938065	23.491494813194237
44-45	22.937535574041732	27.610212890388695	26.9031533724431	22.549098163126473
46-47	22.88336948558459	27.17508064426025	26.48679924088253	23.45475062927263
48-49	23.666080900232537	26.595647074047996	26.88808350658201	22.850188519137458
50-51	23.255703353028494	26.61415794779674	26.581298105795288	23.548840593379484
52-53	22.528245961722316	27.317927426776574	26.500274237286025	23.65355237421509
54-55	22.61742734245029	27.21786986947623	26.459279469988985	23.70542331808449
56-57	23.731351333755036	26.636786885790027	26.22243591012002	23.40942587033492
58-59	22.678149864056344	26.952766741049878	27.25041129399178	23.118672100902
60-61	22.685485347028195	27.61294216188761	26.43707251095117	23.264499980133024
62-63	23.23833045212108	26.81981503861952	25.95664810074552	23.98520640851388
64-65	22.201946219636397	27.069952900450694	26.474785288037506	24.2533155918754
66-67	23.209054878613152	26.485172056992102	26.441063881532205	23.864709182862537
68-69	22.318599399465356	26.70256296643429	26.771420782227338	24.207416851873013
70-71	22.391952690522274	26.316407666341533	26.944896255177024	24.346743387959165
72-73	23.043147969638742	26.53070279035028	27.228785102810775	23.197364137200204
74-75	23.257540853554705	26.952119029042144	26.77811928023503	23.012220837168123
76-77	22.842246589174525	26.96338478732425	26.353742916419737	23.84062570708149
78-79	22.651211270393347	26.85060120576353	26.57179432745243	23.92639319639069
80-81	22.307540464905788	27.129419240376322	27.262681232559334	23.300359062158556
82-83	23.233221200467934	26.00892052289947	28.246519325326215	22.511338951306378
84-85	23.546341414369998	26.728655557415664	26.59973900702338	23.125264021190958
86-87	22.10752414812386	27.30928041024275	27.29261493892209	23.2905805027113
88-89	23.1252897505673	25.96954874037964	27.063377197770095	23.841784311282968
90-91	22.90046635080097	25.971473212035683	27.379369811658737	23.748690625504608
92-93	23.15297005355971	26.25770940100699	27.190140826984543	23.39917971844876
94-95	21.842017780090682	26.264567736533746	27.963135759113044	23.93027872426252
96-97	22.23138015274037	26.31675168293693	27.33367254236635	24.11819562195635
98-99	21.65076391471402	27.06457439106148	27.171773314802905	24.112888379421594
100	22.497986576750346	26.270799889963893	26.306044827837464	24.925168705448296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10804.0
1	6340.5
2	1754.5
3	1280.5
4	687.0
5	377.0
6	291.0
7	275.5
8	271.5
9	259.0
10	240.0
11	230.0
12	232.5
13	252.0
14	265.0
15	336.5
16	401.0
17	441.5
18	579.0
19	709.0
20	844.5
21	1091.5
22	1507.0
23	1995.5
24	2715.5
25	3789.5
26	5382.0
27	7472.0
28	10851.5
29	17678.5
30	27146.5
31	36247.5
32	48204.5
33	59051.5
34	67151.5
35	76500.5
36	84665.0
37	100343.0
38	107357.0
39	98387.0
40	88582.0
41	79706.5
42	77913.5
43	83056.0
44	91985.5
45	111340.5
46	129212.0
47	140665.0
48	146803.0
49	141416.0
50	134542.0
51	139115.5
52	142138.5
53	136901.0
54	130100.5
55	113674.5
56	100029.5
57	82589.5
58	64986.0
59	60965.0
60	50398.5
61	35323.0
62	26286.5
63	21576.5
64	15596.5
65	7795.5
66	4640.0
67	3356.5
68	2444.5
69	1896.5
70	1468.0
71	1096.5
72	787.0
73	600.5
74	480.0
75	378.0
76	294.5
77	202.5
78	131.0
79	95.0
80	69.5
81	48.5
82	31.0
83	22.5
84	22.5
85	21.0
86	14.0
87	6.5
88	3.5
89	7.5
90	9.0
91	4.0
92	3.5
93	6.0
94	5.0
95	4.0
96	3.0
97	2.0
98	4.0
99	3.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0021975454287437
2	0.44731891203405344
3	0.48268536114732064
4	0.46754191749648716
5	0.43401680953992505
6	0.4839552516002207
7	0.5004320802265992
8	0.5050036858570395
9	0.519639173326713
10-11	0.5324333196396812
12-13	0.5304808630683474
14-15	0.5279728294238698
16-17	0.5327666658835675
18-19	0.5294014561833823
20-21	0.529766549688591
22-23	0.4902888302340599
24-25	0.5275918622879998
26-27	0.5050830540103458
28-29	0.5130992374942775
30-31	0.5157501338147065
32-33	0.5169724033756229
34-35	0.5290998572008185
36-37	0.547910109534401
38-39	0.5377509859112004
40-41	0.5432432621199932
42-43	0.5404971240155968
44-45	0.5500847969349923
46-47	0.5490053900500274
48-49	0.5199090250479542
50-51	0.5357191611865603
52-53	0.5283537965597398
54-55	0.5384970465522791
56-57	0.5252266913194734
58-59	0.5326714240996001
60-61	0.525020334120877
62-63	0.5193534479748104
64-65	0.5310364401414912
66-67	0.5292903407687536
68-69	0.5503705222868949
70-71	0.5335444737859688
72-73	0.5377351122805392
74-75	0.5342270399044027
76-77	0.5206233384277105
78-79	0.5531325340219526
80-81	0.5262584773124546
82-83	0.5251473231661671
84-85	0.5347349960855627
86-87	0.8461915032899687
88-89	1.1152812902594957
90-91	1.1061063317372926
92-93	1.1063920570891952
94-95	1.1108208000436843
96-97	1.1044237268872001
98-99	1.0997727531034536
100	1.096455164295252
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3149878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.427162936881146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.27000786377899	21.3971277780397
2	12.322611661548377	7.005937790214947
3	4.7415968368060035	4.043704375826534
4	2.291933943642331	2.6061271862595645
5	1.3072198598971703	1.8580275975811897
6	0.8094170271162586	1.3805657828231896
7	0.5181607371528921	1.0310887791777794
8	0.3631754679491965	0.825923856165589
9	0.265844841769896	0.6801493169629996
>10	1.5257015633090842	8.360370276409048
>50	0.21760597240551577	4.395405688569052
>100	0.2806649614892875	17.523660876679674
>500	0.054206159247341804	10.728889160465203
>1k	0.030511920566029513	15.15563975118269
>5k	0.001117652767986429	2.3424954733582837
>10k+	2.235305535972858E-4	0.6648863102845394
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	10500	0.33334624388627115	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	10427	0.3310286938097285	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	9562	0.30356731276576426	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	9401	0.2984560036928415	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	9285	0.29477332137943124	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	8775	0.2785822181049552	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	8409	0.26696272046091946	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7274	0.23092957885987964	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5429	0.17235588171986344	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5371	0.17051454056315832	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	5196	0.16495876983172048	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5027	0.15959348266821763	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	4760	0.1511169638951096	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4572	0.1451484787664792	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4480	0.14222773072480902	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4390	0.13937047720578383	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4385	0.13921174089917132	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4201	0.13337024481583096	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4184	0.13283054137334843	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4116	0.1306717276034183	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4104	0.13029076046754828	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4083	0.12962406797977574	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	3970	0.126036627450333	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	3822	0.1213380327746027	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	3810	0.12095706563873268	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	3720	0.11809981211970749	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	3713	0.11787758129044998	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	3627	0.1151473168167148	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	3605	0.11444887706761978	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	3563	0.1131154920920747	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	3346	0.10622633638509174	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	3327	0.10562313841996421	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	3310	0.10508343497748165	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	3191	0.10130551088010392	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	3174	0.1007658074376214	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	3164	0.10044833482439636	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	3160	0.10032134577910637	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1747261322502015E-5
2	0.0	0.0	0.0	0.0	3.1747261322502015E-5
3	0.0	0.0	0.0	0.0	3.1747261322502015E-5
4	0.0	0.0	0.0	0.0	3.1747261322502015E-5
5	0.0	0.0	0.0	0.0	3.1747261322502015E-5
6	0.0	0.0	0.0	0.0	3.1747261322502015E-5
7	0.0	0.0	0.0	0.0	3.1747261322502015E-5
8	0.0	0.0	0.0	0.0	3.1747261322502015E-5
9	0.0	0.0	0.0	0.0	3.1747261322502015E-5
10-11	0.0	0.0	0.0	3.1747261322502015E-5	6.349452264500403E-5
12-13	0.0	0.0	0.0	3.1747261322502015E-5	2.539780905800161E-4
14-15	0.0	0.0	0.0	6.349452264500403E-5	3.3334624388627114E-4
16-17	0.0	0.0	0.0	6.349452264500403E-5	4.1271439719252616E-4
18-19	0.0	0.0	0.0	6.349452264500403E-5	4.762089198375302E-4
20-21	0.0	0.0	0.0	1.1111541462875705E-4	5.714507038050362E-4
22-23	0.0	0.0	0.0	1.746099372737611E-4	5.714507038050362E-4
24-25	0.0	0.0	0.0	2.222308292575141E-4	5.714507038050362E-4
26-27	0.0	0.0	0.0	4.444616585150282E-4	6.190715957887893E-4
28-29	0.0	0.0	0.0	0.0013492586062063357	6.349452264500403E-4
30-31	0.0	0.0	0.0	0.005539897100776602	6.349452264500403E-4
32-33	0.0	0.0	0.0	0.01561965257067099	6.984397490950443E-4
34-35	0.0	1.5873630661251007E-5	0.0	0.0322393438730008	7.936815330625503E-4
36-37	0.0	3.1747261322502015E-5	0.0	0.061843665056233926	7.936815330625503E-4
38-39	0.0	3.1747261322502015E-5	0.0	0.10936931525601944	8.730496863688054E-4
40-41	0.0	3.1747261322502015E-5	0.0	0.16302218689104786	8.889233170300564E-4
42-43	0.0	3.1747261322502015E-5	0.0	0.21932595484650516	8.889233170300564E-4
44-45	0.0	3.1747261322502015E-5	0.0	0.2892334242786546	9.524178396750605E-4
46-47	0.0	3.1747261322502015E-5	0.0	0.38193542734036046	0.0010317859929813156
48-49	0.0	3.1747261322502015E-5	0.0	0.4913364898577024	0.0011111541462875704
50-51	0.0	3.1747261322502015E-5	0.0	0.6099760054198924	0.0011429014076100724
52-53	0.0	3.1747261322502015E-5	0.0	0.7284250374141474	0.0013016377142225826
54-55	0.0	3.1747261322502015E-5	0.0	0.8595094794147583	0.0013016377142225826
56-57	0.0	3.1747261322502015E-5	0.0	1.02359519956011	0.0013175113448838337
58-59	0.0	3.1747261322502015E-5	0.0	1.224650605515515	0.0013333849755450846
60-61	0.0	3.1747261322502015E-5	0.0	1.4367540584111511	0.0013333849755450846
62-63	0.0	3.1747261322502015E-5	0.0	1.6588102777313916	0.0013651322368675865
64-65	0.0	3.1747261322502015E-5	0.0	1.8897239829606098	0.0013968794981900885
66-67	0.0	3.1747261322502015E-5	0.0	2.157242280494673	0.0013968794981900885
68-69	0.0	3.1747261322502015E-5	0.0	2.462952533399706	0.0013968794981900885
70-71	0.0	3.1747261322502015E-5	0.0	2.801473580881545	0.0014127531288513396
72-73	0.0	3.1747261322502015E-5	0.0	3.156852424125633	0.0014762476514963438
74-75	0.0	3.1747261322502015E-5	0.0	3.5046119246523197	0.0015238685434800967
76-77	0.0	3.1747261322502015E-5	0.0	3.9092783910995923	0.0015238685434800967
78-79	0.0	3.1747261322502015E-5	0.0	4.351946329349898	0.0016032366967863518
80-81	0.0	3.1747261322502015E-5	0.0	4.834218976100027	0.0016667312194313558
82-83	0.0	3.1747261322502015E-5	0.0	5.338286117748052	0.0018413411567051168
84-85	0.0	3.1747261322502015E-5	0.0	5.834845032093306	0.0018413411567051168
86-87	0.0	3.1747261322502015E-5	0.0	6.364421098213962	0.0019524565713338739
88	0.0	3.1747261322502015E-5	0.0	6.7868977782631585	0.002063571985962631
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	3080	0.0	36.199554	94
TACCCCG	210	0.0	33.55179	5
GTATCAA	7105	0.0	32.54824	1
GGTATCA	4615	0.0	32.417763	1
GTACTAG	3485	0.0	31.689007	1
CAACGCA	6700	0.0	31.40848	5
TATTCTC	1930	0.0	29.692461	5
CAGTACG	350	0.0	29.50954	9
GCATCGT	3505	0.0	29.465563	8
ATCAACG	7165	0.0	29.243195	3
TAGGCAT	4190	0.0	29.147617	5
TATCAAC	8480	0.0	26.870321	2
AACGCAG	8605	0.0	26.457159	6
CTGTGCG	4515	0.0	26.306957	9
CTAGGCA	4775	0.0	26.168974	4
ACTGTGC	4785	0.0	26.096352	8
AGGGCTA	3895	0.0	26.048939	5
TAGTACT	4675	0.0	26.025347	4
ACGCAGA	8235	0.0	25.595455	7
CGCAGAG	8340	0.0	25.329496	8
>>END_MODULE