##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146423_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3149878
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88293832332554	31.0	31.0	34.0	30.0	34.0
2	31.88593716963006	33.0	31.0	34.0	30.0	34.0
3	32.052406474155504	33.0	31.0	34.0	30.0	34.0
4	35.77547162144057	37.0	35.0	37.0	35.0	37.0
5	35.69131471123644	37.0	35.0	37.0	33.0	37.0
6	35.72456711021824	37.0	35.0	37.0	33.0	37.0
7	35.649141966768234	37.0	35.0	37.0	33.0	37.0
8	35.63375533909567	37.0	35.0	37.0	33.0	37.0
9	37.23589707283901	39.0	37.0	39.0	33.0	39.0
10-11	37.18275485590235	39.0	37.0	39.0	33.0	39.0
12-13	37.11416854875014	39.0	37.0	39.0	33.0	39.0
14-15	38.30905514435797	40.0	38.0	41.0	33.0	41.0
16-17	38.31485981361818	40.0	38.0	41.0	33.0	41.0
18-19	38.37662315810327	40.0	38.0	41.0	33.5	41.0
20-21	38.39474893948274	40.0	38.0	41.0	34.0	41.0
22-23	38.361164305411194	40.0	38.0	41.0	34.0	41.0
24-25	38.28557185389403	40.0	37.5	41.0	33.0	41.0
26-27	38.13713658116283	40.0	37.0	41.0	33.0	41.0
28-29	38.0702735153552	40.0	37.0	41.0	33.0	41.0
30-31	37.93694597060585	40.0	37.0	41.0	33.0	41.0
32-33	37.80623598755253	40.0	36.5	41.0	33.0	41.0
34-35	37.70402139384446	40.0	36.0	41.0	32.5	41.0
36-37	37.6155695553923	40.0	36.0	41.0	32.0	41.0
38-39	37.49599921012813	40.0	36.0	41.0	32.0	41.0
40-41	37.35799973840257	40.0	36.0	41.0	31.0	41.0
42-43	37.224074392722514	39.5	35.5	41.0	31.0	41.0
44-45	37.008939711315804	39.0	35.0	41.0	31.0	41.0
46-47	36.741212199329624	39.0	35.0	41.0	30.0	41.0
48-49	36.452834681216224	39.0	35.0	41.0	30.0	41.0
50-51	36.24210715462631	39.0	35.0	41.0	29.0	41.0
52-53	36.04375645659927	38.0	35.0	40.5	29.0	41.0
54-55	35.839351238365424	38.0	35.0	40.0	29.0	41.0
56-57	35.56787675586166	37.5	34.0	40.0	28.0	41.0
58-59	35.27262722556239	37.0	34.0	40.0	28.0	41.0
60-61	35.01012848751603	36.5	34.0	40.0	28.0	41.0
62-63	34.734610673810224	36.0	34.0	39.5	27.0	41.0
64-65	34.46041132386715	35.5	33.5	39.0	27.0	41.0
66-67	34.24174110870326	35.0	33.0	39.0	26.5	41.0
68-69	34.05494942978744	35.0	33.0	38.5	26.0	41.0
70-71	33.86769566948307	35.0	33.0	37.5	26.0	40.5
72-73	33.67813626432516	35.0	33.0	37.0	26.0	40.0
74-75	33.49772880092499	35.0	33.0	37.0	26.0	39.5
76-77	33.34828142550283	35.0	33.0	36.0	26.0	39.0
78-79	33.24063376422833	35.0	33.0	36.0	27.0	39.0
80-81	33.104544366480226	35.0	33.0	36.0	27.0	38.0
82-83	32.914241281725836	35.0	33.0	35.0	26.0	37.0
84-85	32.76795323501418	35.0	33.0	35.0	26.0	37.0
86-87	32.63559985497851	35.0	33.0	35.0	26.0	36.5
88-89	32.52547384374887	35.0	33.0	35.0	26.0	36.0
90-91	32.43241484273359	35.0	33.0	35.0	26.0	36.0
92-93	32.353692904931556	35.0	33.0	35.0	26.0	36.0
94-95	32.31347912522327	35.0	33.0	35.0	26.0	35.5
96-97	32.26006832645582	35.0	33.0	35.0	26.0	35.0
98-99	32.21943167322671	35.0	33.0	35.0	25.0	35.0
100	31.718022729769217	35.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4596130705001187
1101	2	0.5869135417269398
1101	3	0.48845004621376376
1101	4	0.3436018712178637
1101	5	0.3550955681236161
1101	6	0.3668600190373894
1101	7	0.39265408727892037
1101	8	0.41665316472865754
1101	9	0.4899479087404117
1101	10-11	0.48907029996508555
1101	12-13	0.5206851899266525
1101	14-15	0.6219460495284181
1101	16-17	0.6161246983279014
1101	18-19	0.6287819365524783
1101	20-21	0.6562811080194635
1101	22-23	0.6692669962652573
1101	24-25	0.7150131450197321
1101	26-27	0.6947767941633032
1101	28-29	0.7248391787013873
1101	30-31	0.7671939758991115
1101	32-33	0.7144747596442755
1101	34-35	0.7173347091981697
1101	36-37	0.7507882304236162
1101	38-39	0.7470786007448709
1101	40-41	0.7329616064273878
1101	42-43	0.7768378680101478
1101	44-45	0.7415038293435074
1101	46-47	0.6852159453488369
1101	48-49	0.6383772811054698
1101	50-51	0.6197611964555563
1101	52-53	0.6944561170586283
1101	54-55	0.6812046906048153
1101	56-57	0.6484553192938165
1101	58-59	0.5596671433827964
1101	60-61	0.5910180577733328
1101	62-63	0.5776249242864466
1101	64-65	0.6249840196433567
1101	66-67	0.5493309250324288
1101	68-69	0.5314671356739211
1101	70-71	0.5642596043451107
1101	72-73	0.5356292000681862
1101	74-75	0.4803846633595441
1101	76-77	0.41025882928767743
1101	78-79	0.4615593923016874
1101	80-81	0.4316009277742481
1101	82-83	0.4256493891477433
1101	84-85	0.4349469731285325
1101	86-87	0.4599085349859777
1101	88-89	0.4760915312443572
1101	90-91	0.49217387277734304
1101	92-93	0.4296816103764556
1101	94-95	0.5069284477883045
1101	96-97	0.52219380841251
1101	98-99	0.45548519963172396
1101	100	0.549639306987352
1102	1	0.4513250128356674
1102	2	0.5105702209755485
1102	3	0.4615076339010713
1102	4	0.32002728431275784
1102	5	0.2774315367678213
1102	6	0.3280591660652519
1102	7	0.34616265278395986
1102	8	0.3664267119145279
1102	9	0.3772869749422938
1102	10-11	0.39848772311025016
1102	12-13	0.46914535890947207
1102	14-15	0.6288775747526785
1102	16-17	0.5856375855197484
1102	18-19	0.5962953886447409
1102	20-21	0.5371132599578132
1102	22-23	0.5331828018139433
1102	24-25	0.5464909989144573
1102	26-27	0.632800530979587
1102	28-29	0.5787623342186379
1102	30-31	0.6670862277887935
1102	32-33	0.6628456795543656
1102	34-35	0.6356132057167656
1102	36-37	0.7094031833438663
1102	38-39	0.7067746818742293
1102	40-41	0.7073204853123798
1102	42-43	0.7502762822928091
1102	44-45	0.6908385781358177
1102	46-47	0.6729855762685943
1102	48-49	0.759236985305705
1102	50-51	0.7860725853628452
1102	52-53	0.7176343151102955
1102	54-55	0.7273812710218195
1102	56-57	0.8195621368765842
1102	58-59	0.7804707814453735
1102	60-61	0.7892579301040072
1102	62-63	0.7151604759308796
1102	64-65	0.6539113168216844
1102	66-67	0.6660534554319071
1102	68-69	0.6506330933508337
1102	70-71	0.6846209343907788
1102	72-73	0.6618368217480466
1102	74-75	0.6160461181400549
1102	76-77	0.6182237190003477
1102	78-79	0.5764045988168647
1102	80-81	0.5313155331463193
1102	82-83	0.6090672131308565
1102	84-85	0.5051701637878452
1102	86-87	0.5323438003841474
1102	88-89	0.5365414137846329
1102	90-91	0.5025765453459883
1102	92-93	0.5569811488247041
1102	94-95	0.565411680992348
1102	96-97	0.4654454519206297
1102	98-99	0.517354226445633
1102	100	0.5956556247882858
1103	1	0.30178601660831816
1103	2	-0.10338720864525897
1103	3	0.24320040504554896
1103	4	0.2253393286170322
1103	5	0.22291821499894127
1103	6	0.26177744349990917
1103	7	0.3216467125044318
1103	8	0.33377680218019634
1103	9	0.3616165325522118
1103	10-11	0.4199568611595197
1103	12-13	0.4251445126928388
1103	14-15	0.528288870592938
1103	16-17	0.5326401203301927
1103	18-19	0.5361473544858555
1103	20-21	0.5753146426235887
1103	22-23	0.48558707067739704
1103	24-25	0.48320877645162597
1103	26-27	0.5052177081022577
1103	28-29	0.5483549753590538
1103	30-31	0.5362665878839223
1103	32-33	0.53189676508444
1103	34-35	0.5633193903931826
1103	36-37	0.5756381029110571
1103	38-39	0.5816960689630548
1103	40-41	0.5235211953050367
1103	42-43	0.5332012892230296
1103	44-45	0.5552203830959428
1103	46-47	0.5370403361603877
1103	48-49	0.4703073341665771
1103	50-51	0.5179730328133232
1103	52-53	0.5452119931133481
1103	54-55	0.572607902734056
1103	56-57	0.508058428343702
1103	58-59	0.43584778938614477
1103	60-61	0.43362091403894
1103	62-63	0.5109139581276949
1103	64-65	0.5055342558963858
1103	66-67	0.5219398827160262
1103	68-69	0.4641600428934467
1103	70-71	0.3693616596030438
1103	72-73	0.4045757147707576
1103	74-75	0.4500348854205001
1103	76-77	0.38483494388740525
1103	78-79	0.40923688282621384
1103	80-81	0.391848204249861
1103	82-83	0.3412969709301379
1103	84-85	0.32581232088773504
1103	86-87	0.334118014120655
1103	88-89	0.41637174043808045
1103	90-91	0.3961543600337549
1103	92-93	0.4301782351364949
1103	94-95	0.4039392640455972
1103	96-97	0.38849560215862766
1103	98-99	0.38219081525575405
1103	100	0.47947622378987376
1104	1	0.306258946828855
1104	2	0.3820819423927837
1104	3	0.3270381679418506
1104	4	0.2209419512712003
1104	5	0.2198944843180115
1104	6	0.2822994353875927
1104	7	0.24977015451490558
1104	8	0.2152883520758735
1104	9	0.26817186003007265
1104	10-11	0.3085513391830119
1104	12-13	0.3832814111165419
1104	14-15	0.42624467293061485
1104	16-17	0.4142910814366232
1104	18-19	0.45943256581848146
1104	20-21	0.43190178386283407
1104	22-23	0.4162821955935314
1104	24-25	0.3890458899932909
1104	26-27	0.4545343084998663
1104	28-29	0.4533603148392018
1104	30-31	0.5762410214681708
1104	32-33	0.5396293556094065
1104	34-35	0.514748086021271
1104	36-37	0.49767484530498507
1104	38-39	0.5502442997024559
1104	40-41	0.5161778713478498
1104	42-43	0.5332169460560294
1104	44-45	0.5584051060276991
1104	46-47	0.5302228817537014
1104	48-49	0.565869064404346
1104	50-51	0.5247275521370085
1104	52-53	0.4759065794652102
1104	54-55	0.4931015815281725
1104	56-57	0.5147750942922755
1104	58-59	0.56716026284262
1104	60-61	0.5556117630624513
1104	62-63	0.5511710610211651
1104	64-65	0.46759013270980887
1104	66-67	0.42118650822612125
1104	68-69	0.49362793369107294
1104	70-71	0.4711966635114777
1104	72-73	0.502473544510579
1104	74-75	0.43708179898554533
1104	76-77	0.4225059765151684
1104	78-79	0.3541944593485056
1104	80-81	0.31080155073441773
1104	82-83	0.3370556618188587
1104	84-85	0.3508084740000186
1104	86-87	0.37691648644460685
1104	88-89	0.40005632026561955
1104	90-91	0.3441731743921892
1104	92-93	0.42893068945085844
1104	94-95	0.36599314047740705
1104	96-97	0.3939274076107466
1104	98-99	0.30555223343399973
1104	100	0.4180399651805402
1105	1	0.07412707100714755
1105	2	-0.3935367225209099
1105	3	0.0316773595954416
1105	4	0.06677139696440548
1105	5	0.15061408650640118
1105	6	0.19691360477560949
1105	7	0.21857449312129518
1105	8	0.23673752294826045
1105	9	0.32418303522834435
1105	10-11	0.30635791494958653
1105	12-13	0.30549900940314956
1105	14-15	0.3764347255197791
1105	16-17	0.4199847071204985
1105	18-19	0.44670407860527916
1105	20-21	0.4290987069723826
1105	22-23	0.3845232681581052
1105	24-25	0.36984448196071895
1105	26-27	0.3905989740921285
1105	28-29	0.42252514371234895
1105	30-31	0.44260118305263774
1105	32-33	0.473374690441247
1105	34-35	0.43589481616249515
1105	36-37	0.45977830601155034
1105	38-39	0.38120882321913996
1105	40-41	0.4644607713860154
1105	42-43	0.46922859837377473
1105	44-45	0.5203296469327441
1105	46-47	0.48159380503288673
1105	48-49	0.49907101317231906
1105	50-51	0.5270866428533267
1105	52-53	0.5029632294503656
1105	54-55	0.41299587305876884
1105	56-57	0.4860843101199208
1105	58-59	0.4840526777033034
1105	60-61	0.4560700628207073
1105	62-63	0.5285887314058328
1105	64-65	0.524050595409669
1105	66-67	0.47421038540497307
1105	68-69	0.40927188593671815
1105	70-71	0.4074654302106637
1105	72-73	0.3967253151539438
1105	74-75	0.4010592789465335
1105	76-77	0.3903746308262086
1105	78-79	0.3268528630127818
1105	80-81	0.3926394679488894
1105	82-83	0.3132831841904675
1105	84-85	0.30251868223616185
1105	86-87	0.34227231154321913
1105	88-89	0.3803777927623315
1105	90-91	0.3069777356543284
1105	92-93	0.3516293832037789
1105	94-95	0.3191519364399156
1105	96-97	0.29849291081275453
1105	98-99	0.4149199441087461
1105	100	0.3708289935532463
1106	1	0.25191127491072507
1106	2	0.35272145954265355
1106	3	0.2908941440891155
1106	4	0.2312682868871221
1106	5	0.22292507041814957
1106	6	0.1941264039245567
1106	7	0.23116536414140398
1106	8	0.27581482037643923
1106	9	0.2592098886716556
1106	10-11	0.3165764539476186
1106	12-13	0.2594463861809757
1106	14-15	0.35394547285130784
1106	16-17	0.3106636050377105
1106	18-19	0.30118941737942606
1106	20-21	0.32065041126298155
1106	22-23	0.26022397829572697
1106	24-25	0.29826122040857683
1106	26-27	0.323050318352081
1106	28-29	0.30046683621363
1106	30-31	0.3422435340884604
1106	32-33	0.30118967756033044
1106	34-35	0.28836223676323414
1106	36-37	0.3724309419360168
1106	38-39	0.38522298627690077
1106	40-41	0.3260302787354874
1106	42-43	0.2951619391167739
1106	44-45	0.32649637247983065
1106	46-47	0.3160958552625175
1106	48-49	0.2997095416115627
1106	50-51	0.3320428993285205
1106	52-53	0.26981873688716007
1106	54-55	0.24011987523403633
1106	56-57	0.3178407198165303
1106	58-59	0.289494578601591
1106	60-61	0.2744936089042369
1106	62-63	0.24232852491417134
1106	64-65	0.25405887174807873
1106	66-67	0.33875975167958927
1106	68-69	0.3278946794093258
1106	70-71	0.2570131888512037
1106	72-73	0.2973302222088847
1106	74-75	0.29593926211599353
1106	76-77	0.28081805996924203
1106	78-79	0.22706773025396387
1106	80-81	0.25540469780086283
1106	82-83	0.2551788030898905
1106	84-85	0.3506707564810654
1106	86-87	0.31589885616200775
1106	88-89	0.26297370500535067
1106	90-91	0.2723352059924835
1106	92-93	0.21641421825257368
1106	94-95	0.250085832908276
1106	96-97	0.29789193353001053
1106	98-99	0.2920921951537139
1106	100	0.35028565292932257
1107	1	0.08351800340428994
1107	2	-0.46649961402518514
1107	3	-0.014547162755782495
1107	4	0.023102048074932213
1107	5	0.12243939776151791
1107	6	0.12645323849519485
1107	7	0.11630497575746546
1107	8	0.11690635039948916
1107	9	0.11480934749355498
1107	10-11	0.14549043592376165
1107	12-13	0.10098882086570882
1107	14-15	0.14508158068606036
1107	16-17	0.23788257016950354
1107	18-19	0.2501438528042357
1107	20-21	0.19999722008525822
1107	22-23	0.1742157835332776
1107	24-25	0.13167023773090136
1107	26-27	0.16780700020565575
1107	28-29	0.23650638769878185
1107	30-31	0.21485207807268125
1107	32-33	0.27447303671911527
1107	34-35	0.2713205228783977
1107	36-37	0.22176634130541117
1107	38-39	0.30537337134593656
1107	40-41	0.29191649642476136
1107	42-43	0.20768365947725442
1107	44-45	0.18384585250172591
1107	46-47	0.23963968529250224
1107	48-49	0.2342667700396035
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2216	80-81	-0.718241852586111
2216	82-83	-0.642181744184164
2216	84-85	-0.6429126798138043
2216	86-87	-0.6214305619581566
2216	88-89	-0.6124356540656919
2216	90-91	-0.6462877300964749
2216	92-93	-0.6464545846656478
2216	94-95	-0.6158574820791749
2216	96-97	-0.6423245798977817
2216	98-99	-0.6932997807987498
2216	100	-0.7716377222839519
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	39.0
14	219.0
15	611.0
16	1446.0
17	2944.0
18	5305.0
19	8446.0
20	12056.0
21	17319.0
22	23734.0
23	28183.0
24	29161.0
25	29373.0
26	31313.0
27	37605.0
28	46811.0
29	58737.0
30	72274.0
31	89637.0
32	112146.0
33	142597.0
34	196086.0
35	273515.0
36	406234.0
37	697398.0
38	581919.0
39	244248.0
40	514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.66648197862805	22.318940896123298	14.191915744152558	23.822661381096093
2	14.711154486742743	20.590517457598168	40.47100940837769	24.2273186472814
3	17.739960461245257	27.233642800654383	30.208129287346804	24.818267450753552
4	12.314173541659455	17.76734827513605	39.328155560558955	30.590322622645544
5	12.541459178959835	38.86693853707475	35.08487087213889	13.506731411826522
6	28.664380017257812	39.420161669753554	18.065017121298034	13.8504411916906
7	25.266789126902538	32.93711542014803	24.331384255711193	17.46471119723823
8	26.04005615455583	34.71788431170985	19.975408571379592	19.266650962354735
9	25.991959053652238	14.554309722471793	22.203304381947493	37.25042684192848
10-11	25.53544930946532	26.177172576207713	27.260468500684787	21.02690961364218
12-13	25.928369289223262	24.092393419681652	27.853221616837224	22.12601567425786
14-15	23.278552362494853	24.849193843101904	25.900971338019474	25.971282456383772
16-17	21.469850929365904	27.292857092967072	27.344209853253336	23.893082124413688
18-19	20.931143999862854	27.12255839749984	30.148310505994203	21.79798709664311
20-21	22.592723461900256	26.253929915358597	29.21455345716674	21.938793165574403
22-23	23.07162246915058	25.855130225773777	29.0372910638945	22.035956241181147
24-25	22.465505013210034	26.028785876786337	29.70986495349979	21.795844156503836
26-27	22.33002542481441	26.26097444168825	28.801916139225188	22.607083994272152
28-29	21.444560709970354	26.884485684842396	29.41452018141655	22.256433423770698
30-31	22.40567729924778	26.29941540593001	29.144668460175282	22.150238834646927
32-33	22.93288819439991	26.777370425140273	28.163884442508568	22.125856937951248
34-35	22.751760075990582	26.51519240195767	29.130956130169427	21.60209139188232
36-37	22.533793372314737	26.13928221981931	28.81978286143146	22.507141546434497
38-39	22.718117971553184	25.897288720388534	29.543493430539215	21.841099877519067
40-41	22.23849100738667	25.283353593473745	29.937175045390862	22.54098035374872
42-43	22.407844818007206	25.62339476483007	28.944854516881758	23.023905900280965
44-45	22.585049325719915	26.590918759393222	28.58036089016781	22.243671024719053
46-47	22.664607962594108	26.265556951729558	27.912493753726338	23.15734133195
48-49	23.64348689893738	25.93930394979585	27.854500788765463	22.562708362501304
50-51	23.042229420033	26.196606058489575	27.310379530632538	23.450784990844884
52-53	22.22436868983497	26.80381589382192	27.25970656641305	23.71210884993006
54-55	22.447319981230947	26.797711394706948	27.034134150468624	23.72083447359348
56-57	23.517051793182837	26.11420329829066	26.907298514162786	23.461446394363712
58-59	22.339488140536663	26.514995784744666	27.91335574802567	23.232160326693002
60-61	22.512119133635323	27.160732911739814	27.05461757285886	23.272530381766003
62-63	23.04641640088918	26.33517869580981	26.552314089625057	24.06609081367596
64-65	22.022567223238486	26.577140447979254	27.054365280179105	24.34592704860315
66-67	22.772294673000033	26.078216362665472	27.120320215576605	24.02916874875789
68-69	22.226772735967288	25.966660079004328	27.483298688578977	24.32326849644941
70-71	22.256075686143785	25.635284219883488	27.62776005206597	24.480880041906758
72-73	22.85613919015276	25.763537508436833	27.903096564374874	23.47722673703553
74-75	22.953864293786047	26.325082253381527	27.3456638931664	23.375389559666022
76-77	22.64858329878068	26.347251968548935	26.805918631829122	24.198246100841263
78-79	22.27523510145757	26.344935264655117	27.157335548032478	24.22249408585483
80-81	21.981857076457693	26.704173132751365	27.742360707201293	23.57160908358965
82-83	22.847998125608964	25.58331055427559	28.772854855653797	22.795836464461654
84-85	23.269538095299325	26.18379638735474	27.059042225313533	23.487623292032396
86-87	21.772901918532114	26.851131159728244	27.671086827417202	23.704880094322444
88-89	22.600083724239596	25.623824165761928	27.507508432351717	24.268583677646756
90-91	22.282163255541573	25.517534458774165	27.830856539074972	24.369445746609287
92-93	22.633507186815287	25.94187745885413	27.391734217851422	24.032881136479165
94-95	21.229050456691315	25.885349368061135	28.22228501220701	24.663315163040544
96-97	21.68249187785204	25.79941207491076	27.683785325747902	24.8343107214893
98-99	21.03654205119389	26.593465414336965	27.557555464862237	24.812437069606908
100	21.86789240592	25.756316325629246	26.70946077767843	25.66633049077232
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	86.0
2	77.5
3	106.0
4	83.5
5	65.0
6	124.0
7	339.5
8	621.5
9	754.0
10	618.0
11	429.5
12	401.0
13	489.0
14	643.0
15	940.5
16	1285.5
17	1701.0
18	2250.0
19	2947.0
20	3744.0
21	4504.5
22	5320.5
23	6118.5
24	7012.5
25	7961.0
26	9182.5
27	10717.5
28	13847.0
29	20414.0
30	28916.0
31	37139.5
32	48641.0
33	58680.0
34	65234.0
35	73747.0
36	81531.0
37	97673.5
38	105328.0
39	95888.0
40	86659.5
41	78601.0
42	76247.5
43	80990.5
44	89625.0
45	109761.5
46	128931.0
47	140960.0
48	147011.0
49	140497.5
50	133021.0
51	136705.5
52	139470.5
53	135651.0
54	131908.0
55	114897.5
56	99996.5
57	83439.5
58	65791.5
59	62782.0
60	51734.0
61	35412.0
62	25601.5
63	20820.5
64	15070.0
65	7110.0
66	4078.0
67	2964.0
68	2152.5
69	1631.0
70	1262.0
71	972.0
72	684.5
73	546.5
74	438.0
75	277.5
76	205.0
77	144.5
78	94.0
79	58.5
80	32.0
81	10.5
82	4.5
83	3.5
84	1.0
85	1.0
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013429091539418351
2	0.004190638494570266
3	0.0018095938953826148
4	0.028731271496864323
5	0.0029524953029926872
6	0.0
7	3.809671358700242E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009682914703363113
16-17	0.0030477370869601934
18-19	0.0
20-21	3.1747261322502015E-5
22-23	6.825661184337934E-4
24-25	0.0
26-27	7.936815330625504E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.4446165851502826E-4
36-37	0.0
38-39	0.0
40-41	4.7620891983753025E-4
42-43	8.889233170300565E-4
44-45	0.0
46-47	0.0
48-49	6.031979651275383E-4
50-51	1.5873630661251007E-5
52-53	0.0
54-55	3.809671358700242E-4
56-57	1.2698904529000806E-4
58-59	3.0159898256376915E-4
60-61	1.1111541462875706E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0016032366967863518
70-71	8.889233170300565E-4
72-73	0.0
74-75	2.3810445991876512E-4
76-77	0.0030477370869601934
78-79	4.6033528917627925E-4
80-81	0.0034921987454752217
82-83	0.0017460993727376108
84-85	0.006079600543259136
86-87	0.008143172529221768
88-89	0.008016183483931759
90-91	0.006619303985741671
92-93	0.009238453044848086
94-95	0.0015238685434800967
96-97	0.004984320027632817
98-99	0.002174687400591388
100	0.08622556175191547
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3149878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.22495926968972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.03741238907456	15.343536784319545
2	15.211905041405437	6.761679399192472
3	5.816875444553275	3.878394594961644
4	2.9236670309721076	2.5991352272595907
5	1.6637611033842208	1.8488511378604169
6	1.0432065910936994	1.3911134398157599
7	0.6543480853789665	1.0180001682022888
8	0.48155670691858377	0.8562062557849146
9	0.34199631974122596	0.6840768848969275
>10	2.016111049134198	8.627059333155298
>50	0.2987949115615785	4.703541558660575
>100	0.3837746555756936	18.636165519613463
>500	0.07906565033634957	12.26493216886559
>1k	0.04566968972496725	18.078289570076368
>5k	0.0015698955842957496	2.583319675596015
>10k+	2.8543556078104536E-4	0.7256982817391242
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	11564	0.3671253299334133	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	11315	0.35922026186411027	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	9642	0.3061070936715644	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	9594	0.30458322512808433	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	9469	0.3006148174627716	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	9284	0.2947415741181087	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7738	0.24566030811352058	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7152	0.2270564129785344	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6603	0.20962716651248078	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5807	0.1843563464997692	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	5580	0.17714971817956124	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5565	0.17667350925972372	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5010	0.1590537792257351	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	4920	0.15619652570670992	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4786	0.15194239268949464	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4779	0.15172016186023712	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4761	0.15114871115643208	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4689	0.14886290834121194	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4619	0.1466406000486368	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4560	0.14476751163060916	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4371	0.1387672792406563	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4363	0.1385133011500763	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4336	0.13765612509436873	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4285	0.13603701476692112	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4099	0.13013202416093578	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4040	0.12825893574290814	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	3998	0.12692555076736306	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	3989	0.12663982541546054	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	3890	0.12349684654453284	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	3877	0.1230841321473403	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	3825	0.1214332745585702	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	3809	0.12092531837741018	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	3758	0.11930620804996257	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3712	0.11784583402912749	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	3623	0.11502032777142479	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	3578	0.11359170101191221	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	3568	0.1132742283986872	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	3535	0.11222656877504461	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	3526	0.11194084342314212	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	3432	0.10895660085882691	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	3431	0.10892485359750442	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	3414	0.10838515015502187	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	3307	0.10498819319351416	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	3230	0.1025436540716815	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	3185	0.1011150273121689	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.349452264500403E-5
2	0.0	0.0	0.0	3.1747261322502015E-5	6.349452264500403E-5
3	0.0	0.0	0.0	3.1747261322502015E-5	6.349452264500403E-5
4	0.0	0.0	0.0	3.1747261322502015E-5	6.349452264500403E-5
5	0.0	0.0	0.0	3.1747261322502015E-5	9.524178396750604E-5
6	0.0	0.0	0.0	3.1747261322502015E-5	9.524178396750604E-5
7	0.0	0.0	0.0	3.1747261322502015E-5	9.524178396750604E-5
8	0.0	0.0	0.0	3.1747261322502015E-5	9.524178396750604E-5
9	0.0	0.0	0.0	6.349452264500403E-5	9.524178396750604E-5
10-11	0.0	0.0	0.0	1.2698904529000806E-4	1.2698904529000806E-4
12-13	0.0	0.0	0.0	1.2698904529000806E-4	4.762089198375302E-4
14-15	0.0	0.0	0.0	1.4286267595125905E-4	4.920825504987812E-4
16-17	0.0	0.0	0.0	1.5873630661251007E-4	5.555770731437852E-4
18-19	0.0	0.0	0.0	1.5873630661251007E-4	5.714507038050362E-4
20-21	0.0	0.0	0.0	2.2223082925751408E-4	6.349452264500403E-4
22-23	0.0	0.0	0.0	2.857253519025181E-4	6.349452264500403E-4
24-25	0.0	0.0	0.0	3.1747261322502015E-4	6.349452264500403E-4
26-27	0.0	0.0	0.0	5.397034424825342E-4	6.349452264500403E-4
28-29	0.0	0.0	0.0	0.0014286267595125905	6.508188571112913E-4
30-31	0.0	0.0	0.0	0.005619265254082857	7.301870104175464E-4
32-33	0.0	0.0	0.0	0.015889504291912257	7.301870104175464E-4
34-35	0.0	0.0	0.0	0.03281079457680583	7.301870104175464E-4
36-37	0.0	0.0	0.0	0.063113555509134	7.301870104175464E-4
38-39	0.0	0.0	0.0	0.11138526634999832	7.778079024012993E-4
40-41	0.0	0.0	0.0	0.1659905558247018	7.936815330625503E-4
42-43	0.0	0.0	0.0	0.2230721316825604	8.095551637238013E-4
44-45	0.0	0.0	0.0	0.29445584876620623	8.254287943850523E-4
46-47	0.0	0.0	0.0	0.3887610885246984	8.254287943850523E-4
48-49	0.0	0.0	0.0	0.4999241240454392	8.571760557075544E-4
50-51	0.0	0.0	0.0	0.6204208543949956	9.206705783525584E-4
52-53	0.0	0.0	0.0	0.740155650472812	9.524178396750604E-4
54-55	0.0	0.0	0.0	0.8726845928635967	0.0010476596236425665
56-57	0.0	0.0	0.0	1.0383259288137507	0.0010952805156263195
58-59	0.0	0.0	0.0	1.2410956868805711	0.0011429014076100724
60-61	0.0	0.0	0.0	1.4554055744381211	0.0011429014076100724
62-63	0.0	0.0	0.0	1.6796682284202753	0.0012063959302550766
64-65	0.0	0.0	0.0	1.9134868080605028	0.0012381431915775786
66-67	0.0	0.0	0.0	2.1847195351692985	0.0012540168222388297
68-69	0.0	0.0	0.0	2.4933505361160018	0.0012698904529000806
70-71	0.0	0.0	0.0	2.8362209583990237	0.0013016377142225826
72-73	0.0	0.0	0.0	3.1963460172108253	0.0013968794981900888
74-75	0.0	0.0	0.0	3.5495660466849825	0.0014762476514963438
76-77	0.0	0.0	0.0	3.962582042860073	0.0014921212821575947
78-79	0.0	0.0	0.0	4.415075758489694	0.0015238685434800967
80-81	0.0	0.0	0.0	4.907205929880458	0.0015714894354638496
82-83	0.0	0.0	0.0	5.422162382162103	0.0016826048500926069
84-85	0.0	0.0	0.0	5.929737596186265	0.0016826048500926069
86-87	0.0	0.0	0.0	6.4693457968848325	0.0018095938953826148
88	0.0	0.0	0.0	6.900298995707136	0.001936582940672623
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6845	0.0	45.127773	1
GTATCAA	11860	0.0	38.929493	1
TACCCCG	295	0.0	36.64136	5
GTGGTAT	1625	0.0	34.72001	1
GGGCACC	3055	0.0	33.575428	94
ATCAACG	13870	0.0	32.13286	3
CAACGCA	13905	0.0	32.040787	5
TATTCTC	2000	0.0	31.01771	5
TATCAAC	15245	0.0	30.190672	2
GTACTAG	3130	0.0	29.742184	1
AACGCAG	15980	0.0	29.085629	6
ACGCAGA	16585	0.0	26.721148	7
CGCAGAG	16780	0.0	26.382616	8
TAGGCAT	4225	0.0	26.140085	5
TCAACGC	17305	0.0	26.080572	4
TAACCAC	1130	0.0	25.369808	5
GCAGAGT	17705	0.0	24.769686	9
TAATAAT	1695	0.0	24.407904	3
AGGGCTA	4490	0.0	24.387955	5
TTCTGGC	1775	0.0	24.097822	9
>>END_MODULE