##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146422_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4606533 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.321735674095898 31.0 30.0 34.0 26.0 34.0 2 30.353634718344576 31.0 30.0 34.0 26.0 34.0 3 30.64116701215426 31.0 30.0 34.0 26.0 34.0 4 34.096735874897675 37.0 35.0 37.0 29.0 37.0 5 34.3581046743831 37.0 35.0 37.0 30.0 37.0 6 34.44769157194793 37.0 35.0 37.0 30.0 37.0 7 34.351418952170754 37.0 35.0 37.0 30.0 37.0 8 34.325859382750544 37.0 35.0 37.0 30.0 37.0 9 35.691258480076016 39.0 35.0 39.0 30.0 39.0 10-11 35.597154845086315 39.0 35.0 39.0 29.5 39.0 12-13 35.56942585671263 39.0 35.0 39.0 29.0 39.0 14-15 36.56583248182527 40.0 36.0 41.0 28.5 41.0 16-17 36.40322483307945 40.0 36.0 41.0 27.5 41.0 18-19 36.34480323922568 39.5 36.0 41.0 27.0 41.0 20-21 36.684644503794935 40.0 36.0 41.0 29.0 41.0 22-23 36.39634102263025 39.5 35.5 41.0 27.5 41.0 24-25 36.50423300994479 39.0 35.0 41.0 28.5 41.0 26-27 36.580610081377905 39.0 35.0 41.0 29.0 41.0 28-29 36.42158614732598 39.0 35.0 41.0 28.5 41.0 30-31 36.36910774328546 39.0 35.0 41.0 28.5 41.0 32-33 36.478165140681725 39.0 35.0 41.0 29.0 41.0 34-35 36.527223076443825 39.0 35.0 41.0 29.0 41.0 36-37 36.45462259794948 39.0 35.0 41.0 29.0 41.0 38-39 36.27326321118289 39.0 35.0 41.0 28.0 41.0 40-41 36.22633247173091 39.0 35.0 41.0 28.0 41.0 42-43 36.06617536442266 39.0 35.0 41.0 28.0 41.0 44-45 35.852478968456325 38.5 34.0 40.5 27.5 41.0 46-47 35.71220188805768 38.0 34.0 40.0 27.0 41.0 48-49 35.57023774713 38.0 34.0 40.0 27.0 41.0 50-51 35.33998475643179 38.0 33.5 40.0 26.5 41.0 52-53 35.1386238848175 38.0 33.0 40.0 26.0 41.0 54-55 34.94238074491163 37.0 33.0 40.0 26.0 41.0 56-57 34.77609386495223 37.0 33.0 40.0 26.0 41.0 58-59 34.49384472009643 36.0 33.0 40.0 26.0 41.0 60-61 34.24153327459067 36.0 33.0 39.5 26.0 41.0 62-63 33.96838837364239 35.0 32.5 39.0 26.0 41.0 64-65 33.676163830802906 35.0 32.0 39.0 25.5 41.0 66-67 33.3943810887711 35.0 32.0 38.0 25.0 40.0 68-69 33.11277548646672 35.0 32.0 37.5 25.0 40.0 70-71 33.080351752608735 35.0 32.0 37.0 25.0 40.0 72-73 32.89996804538251 35.0 32.0 37.0 25.0 40.0 74-75 32.6490268277683 35.0 31.0 36.0 24.0 39.0 76-77 32.43209730615193 35.0 31.0 36.0 24.0 39.0 78-79 32.22907097376704 35.0 31.0 36.0 24.0 39.0 80-81 32.03196471185596 35.0 31.0 35.0 24.0 37.5 82-83 31.80198166386738 35.0 31.0 35.0 23.0 37.0 84-85 31.629988540188467 34.0 31.0 35.0 23.0 37.0 86-87 31.46647641512608 34.5 31.0 35.0 23.0 36.0 88-89 31.485895140662187 35.0 31.0 35.0 23.0 36.0 90-91 31.416732931252202 35.0 31.0 35.0 23.0 36.0 92-93 31.274115370496638 34.5 31.0 35.0 23.0 36.0 94-95 31.25935752549694 34.0 31.0 35.0 23.0 35.0 96-97 31.17465553812379 34.0 31.0 35.0 23.0 35.0 98-99 31.098514652993913 34.0 31.0 35.0 22.5 35.0 100 30.59724005016354 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7160529622411005 1101 2 0.8747950831484843 1101 3 0.8612973480178532 1101 4 0.938303247611131 1101 5 0.856953348019573 1101 6 0.6413227530876142 1101 7 0.6877927779376591 1101 8 0.7104533304659384 1101 9 0.9009273948444942 1101 10-11 0.9062024483930458 1101 12-13 0.7485567468205332 1101 14-15 1.0199520921526357 1101 16-17 1.1179369951636104 1101 18-19 1.2970684202617733 1101 20-21 0.9216423667422262 1101 22-23 1.2326146291501558 1101 24-25 1.0807422257785362 1101 26-27 1.0265984728124167 1101 28-29 1.2267031685948027 1101 30-31 1.1784146859370068 1101 32-33 0.9957623922979906 1101 34-35 0.9387809804462819 1101 36-37 0.9339060112114481 1101 38-39 1.0457660634583945 1101 40-41 0.9583463399381174 1101 42-43 0.9581749660554948 1101 44-45 0.9377639379629983 1101 46-47 0.949404129735747 1101 48-49 0.9487399979637772 1101 50-51 0.9786032001334135 1101 52-53 0.9550569022122559 1101 54-55 0.9705683566692329 1101 56-57 0.824720450550096 1101 58-59 0.768985233674627 1101 60-61 0.8460819698293065 1101 62-63 0.8119241590285782 1101 64-65 0.8288486733240603 1101 66-67 0.7929393678461096 1101 68-69 0.8232111164693379 1101 70-71 0.7609362005439877 1101 72-73 0.722688982657175 1101 74-75 0.7519146527373408 1101 76-77 0.8069864833209124 1101 78-79 0.7748466825761042 1101 80-81 0.8065531100502312 1101 82-83 0.8121207881711427 1101 84-85 0.6980922776913943 1101 86-87 0.7630922816116126 1101 88-89 0.7375777425477068 1101 90-91 0.7214458855691674 1101 92-93 0.7935185544192365 1101 94-95 0.7872323045619183 1101 96-97 0.6931549121264062 1101 98-99 0.7250378580810839 1101 100 0.8684213000847052 1102 1 0.7009386089757577 1102 2 0.8374899984374338 1102 3 0.8381163093472601 1102 4 0.9191704436306267 1102 5 0.8340038211006373 1102 6 0.6108573653570275 1102 7 0.678621520618691 1102 8 0.7109494185555647 1102 9 0.9119996571101652 1102 10-11 0.8577401335813306 1102 12-13 0.8176698144440948 1102 14-15 1.0639595056981435 1102 16-17 1.2253914429300465 1102 18-19 1.1730613892554445 1102 20-21 0.9489924361907853 1102 22-23 1.2477117045288466 1102 24-25 1.1211574294996325 1102 26-27 0.9610261292969398 1102 28-29 1.1274731042290043 1102 30-31 1.2120587673969325 1102 32-33 1.0907607363845386 1102 34-35 0.9197812499932425 1102 36-37 0.9400054730261544 1102 38-39 0.9314395698949482 1102 40-41 0.9066153034675182 1102 42-43 0.9462004263039034 1102 44-45 0.9984950236029988 1102 46-47 0.9565325105772757 1102 48-49 0.8726502429811092 1102 50-51 0.9747355084234854 1102 52-53 0.8275756639790899 1102 54-55 0.9382719603095069 1102 56-57 0.8583178180920825 1102 58-59 0.8096327742708525 1102 60-61 0.8623542198553125 1102 62-63 0.8304698826890018 1102 64-65 0.7673392377786996 1102 66-67 0.7844570985664845 1102 68-69 0.8302607029900244 1102 70-71 0.8003452805168081 1102 72-73 0.7255548452373688 1102 74-75 0.8344207368299834 1102 76-77 0.6727701200806422 1102 78-79 0.7275862446001682 1102 80-81 0.7851659395113515 1102 82-83 0.7351675166231857 1102 84-85 0.7550232323335919 1102 86-87 0.7223747546390697 1102 88-89 0.7251009213098385 1102 90-91 0.7766631482337871 1102 92-93 0.872921716964413 1102 94-95 0.8278620006204349 1102 96-97 0.7760503948497863 1102 98-99 0.7992646930329101 1102 100 0.828914726306131 1103 1 0.5019100477584217 1103 2 0.6943384197262255 1103 3 0.6287032976990758 1103 4 0.6546655303878879 1103 5 0.6408358489526691 1103 6 0.4421430721438284 1103 7 0.5121364447258827 1103 8 0.5403262068875847 1103 9 0.5643983977858653 1103 10-11 0.6873409185271271 1103 12-13 0.6032766730082173 1103 14-15 0.7527760744061922 1103 16-17 0.9907661957817453 1103 18-19 1.0341658554824775 1103 20-21 0.7273374552702379 1103 22-23 0.9099990195056407 1103 24-25 0.817462794701342 1103 26-27 0.7730618663135402 1103 28-29 0.9686065159684389 1103 30-31 0.9699955273155183 1103 32-33 0.7858381435663091 1103 34-35 0.6569687688558261 1103 36-37 0.6445826095801266 1103 38-39 0.7772237525550665 1103 40-41 0.7305108604865467 1103 42-43 0.6826210339287968 1103 44-45 0.7254876069984206 1103 46-47 0.6886081720000945 1103 48-49 0.6684485600381649 1103 50-51 0.7715502344544021 1103 52-53 0.8260012602473878 1103 54-55 0.7392408360654557 1103 56-57 0.7019495947595544 1103 58-59 0.6745488188538431 1103 60-61 0.5849850682302744 1103 62-63 0.5628577664630114 1103 64-65 0.5859233093663789 1103 66-67 0.6167546942606705 1103 68-69 0.647765253554546 1103 70-71 0.6102487978596898 1103 72-73 0.5647337305544013 1103 74-75 0.5953960543264074 1103 76-77 0.5242704881034683 1103 78-79 0.5503899919756989 1103 80-81 0.5812940227381134 1103 82-83 0.5197297133143124 1103 84-85 0.5133128157281064 1103 86-87 0.589784723557301 1103 88-89 0.5579091752413277 1103 90-91 0.5342650258634478 1103 92-93 0.546663883788522 1103 94-95 0.5352921336049619 1103 96-97 0.5602908621487614 1103 98-99 0.5687181552031717 1103 100 0.6457648109793936 1104 1 0.5033648902696406 1104 2 0.6337005846400245 1104 3 0.6144579012917042 1104 4 0.6802252290742601 1104 5 0.6072675038003794 1104 6 0.3550765943304839 1104 7 0.37724681003437865 1104 8 0.3664434213519385 1104 9 0.551689124263639 1104 10-11 0.5988731209674185 1104 12-13 0.5516618872858245 1104 14-15 0.7572938059707752 1104 16-17 0.8467471596486078 1104 18-19 0.8126462758198016 1104 20-21 0.7057178720328565 1104 22-23 0.8500542651565794 1104 24-25 0.7737395304455816 1104 26-27 0.6870512617739166 1104 28-29 0.8658801366486983 1104 30-31 0.8811774584207797 1104 32-33 0.7075566657832439 1104 34-35 0.6302797788987746 1104 36-37 0.6357808299677288 1104 38-39 0.76696208092973 1104 40-41 0.6230687251391913 1104 42-43 0.5715830673406685 1104 44-45 0.694401673074033 1104 46-47 0.648802344386759 1104 48-49 0.6018875129297285 1104 50-51 0.7204894386490253 1104 52-53 0.8019880666905053 1104 54-55 0.6936439136635002 1104 56-57 0.6100402150512849 1104 58-59 0.5917750718872057 1104 60-61 0.5923740324221782 1104 62-63 0.5170814841972344 1104 64-65 0.4668209466879176 1104 66-67 0.6263570005839512 1104 68-69 0.6088595362489002 1104 70-71 0.5444193620189353 1104 72-73 0.5345572148199764 1104 74-75 0.5162150913190402 1104 76-77 0.5632182324998354 1104 78-79 0.4713402769453552 1104 80-81 0.5335579184882704 1104 82-83 0.5405265403129107 1104 84-85 0.5612003854957166 1104 86-87 0.5133812599702665 1104 88-89 0.38261543000148635 1104 90-91 0.44811057400981014 1104 92-93 0.48459877822854125 1104 94-95 0.404457932480085 1104 96-97 0.441963817740298 1104 98-99 0.5307349242370094 1104 100 0.577558681138246 1105 1 0.5045154729633197 1105 2 0.5671937933278386 1105 3 0.5965579266065575 1105 4 0.6301930581146138 1105 5 0.5323073784617165 1105 6 0.2819404380896202 1105 7 0.368603294403961 1105 8 0.4459453026373268 1105 9 0.4673026817238366 1105 10-11 0.5161648610923635 1105 12-13 0.403276085904956 1105 14-15 0.6794047954048352 1105 16-17 0.7964651646121297 1105 18-19 0.8142965160848803 1105 20-21 0.6350042474430992 1105 22-23 0.8823633546204732 1105 24-25 0.7186033934168847 1105 26-27 0.5449325441520614 1105 28-29 0.8553242665410181 1105 30-31 0.7953450932299049 1105 32-33 0.6854265301076836 1105 34-35 0.6171347398566311 1105 36-37 0.543720568185023 1105 38-39 0.6455659629926558 1105 40-41 0.584194377222687 1105 42-43 0.6000387614498379 1105 44-45 0.6431898092227399 1105 46-47 0.6038967232084786 1105 48-49 0.6575147468148757 1105 50-51 0.6560000283193617 1105 52-53 0.6784135246181293 1105 54-55 0.5953612175713374 1105 56-57 0.5407221565197204 1105 58-59 0.5523826735760693 1105 60-61 0.5304526388093578 1105 62-63 0.5096688185582323 1105 64-65 0.46905511435662817 1105 66-67 0.6213814734925407 1105 68-69 0.5424380601367744 1105 70-71 0.5241275480590062 1105 72-73 0.48337802106792793 1105 74-75 0.5243373827804021 1105 76-77 0.5019860376568346 1105 78-79 0.5188987968250487 1105 80-81 0.4959977109516771 1105 82-83 0.48691788346177844 1105 84-85 0.5104968058548849 1105 86-87 0.5580881388296532 1105 88-89 0.47762490988672823 1105 90-91 0.4463223075197469 1105 92-93 0.49982443829827616 1105 94-95 0.4885595640826672 1105 96-97 0.4522231518391031 1105 98-99 0.5346669387870193 1105 100 0.5906748265901598 1106 1 0.4781490037696514 1106 2 0.5142922798173934 1106 3 0.5211928162716895 1106 4 0.5838081987703774 1106 5 0.5040120817604503 1106 6 0.27760745402483167 1106 7 0.3436439183687554 1106 8 0.36498385648452114 1106 9 0.5255551354535442 1106 10-11 0.4706435061315375 1106 12-13 0.4223822470504004 1106 14-15 0.603360397092068 1106 16-17 0.7189279902147874 1106 18-19 0.67787991672256 1106 20-21 0.4748910392541035 1106 22-23 0.7920057026977005 1106 24-25 0.643792855638857 1106 26-27 0.5651868263373174 1106 28-29 0.6964385077523403 1106 30-31 0.6725172044330705 1106 32-33 0.5746858547633025 1106 34-35 0.5225953972136281 1106 36-37 0.429906800402037 1106 38-39 0.551085546217621 1106 40-41 0.5276886747825955 1106 42-43 0.5279199196084576 1106 44-45 0.5141286855424525 1106 46-47 0.5407257885616232 1106 48-49 0.5483830305104291 1106 50-51 0.5977028003581282 1106 52-53 0.538678258083884 1106 54-55 0.5915409734111989 1106 56-57 0.5617578805676047 1106 58-59 0.49196059767186284 1106 60-61 0.5239931380210123 1106 62-63 0.46338330351333923 1106 64-65 0.49937874314319686 1106 66-67 0.49655746856094396 1106 68-69 0.4528288515905672 1106 70-71 0.5310269846635904 1106 72-73 0.4251159883491127 1106 74-75 0.4814978147822515 1106 76-77 0.48727845213833376 1106 78-79 0.4345279942324751 1106 80-81 0.4990830222582616 1106 82-83 0.48348408928604414 1106 84-85 0.432562489963658 1106 86-87 0.4875070358671394 1106 88-89 0.509784319878321 1106 90-91 0.4208624387322857 1106 92-93 0.5004850302767849 1106 94-95 0.5341073178323512 1106 96-97 0.4749054060603477 1106 98-99 0.49956643030109404 1106 100 0.5472060906003762 1107 1 0.4255823039236688 1107 2 0.5405825938900826 1107 3 0.4837370427387846 1107 4 0.6175894970781144 1107 5 0.5039963906303839 1107 6 0.3389517720273929 1107 7 0.28758218396151136 1107 8 0.322159081910371 1107 9 0.47250175874269473 1107 10-11 0.41788782502713673 1107 12-13 0.3564628701300663 1107 14-15 0.5333031820014824 1107 16-17 0.6061916277972585 1107 18-19 0.7330503174552305 1107 20-21 0.520113912481726 1107 22-23 0.7257917877686921 1107 24-25 0.4925903368289184 1107 26-27 0.4299279098090736 1107 28-29 0.5733682963961044 1107 30-31 0.6477497823340599 1107 32-33 0.5660727416394238 1107 34-35 0.4304527519010293 1107 36-37 0.43891486508111655 1107 38-39 0.4953121799117355 1107 40-41 0.31636524244902375 1107 42-43 0.46379352550240327 1107 44-45 0.3949626501209096 1107 46-47 0.3794065105031521 1107 48-49 0.4165563549655573 1107 50-51 0.4698940904985278 1107 52-53 0.4909656093225365 1107 54-55 0.46696990546455197 1107 56-57 0.34186839881444087 1107 58-59 0.41295789839068675 1107 60-61 0.4845350503757473 1107 62-63 0.4630239669490379 1107 64-65 0.4775024020721901 1107 66-67 0.42875024529063666 1107 68-69 0.4679346928152057 1107 70-71 0.42001323712214145 1107 72-73 0.38449886860171745 1107 74-75 0.3544339299346859 1107 76-77 0.33427295468311513 1107 78-79 0.3338459744214006 1107 80-81 0.3858742599670464 1107 82-83 0.38443134149893154 1107 84-85 0.38135539747988645 1107 86-87 0.4011529356072856 1107 88-89 0.42257022946049005 1107 90-91 0.45712128898971827 1107 92-93 0.5127164405058657 1107 94-95 0.3998759088043222 1107 96-97 0.4596388276114105 1107 98-99 0.4472596026894067 1107 100 0.4471417782760625 1108 1 0.18192619331011883 1108 2 0.2957550221147969 1108 3 0.2288310283889068 1108 4 0.32822497387100213 1108 5 0.29412655840333457 1108 6 -0.0276538355531315 1108 7 -0.004848061206182308 1108 8 0.025500872560691334 1108 9 0.06157975998463172 1108 10-11 0.1746047439832168 1108 12-13 0.013457219401807663 1108 14-15 0.19360529863353548 1108 16-17 0.3131912355294233 1108 18-19 0.3383599121329439 1108 20-21 0.041937622543194664 1108 22-23 0.2723671471959861 1108 24-25 0.12520083966676054 1108 26-27 0.15311244732405527 1108 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-1.5014204806156073 2215 80-81 -1.4674205056205167 2215 82-83 -1.3953508883159174 2215 84-85 -1.2168093749577125 2215 86-87 -1.285249235196403 2215 88-89 -1.184805738937719 2215 90-91 -1.4944373196071403 2215 92-93 -3.885839128557336 2215 94-95 -1.9725212828814662 2215 96-97 -1.4208787077428333 2215 98-99 -3.0133315852962568 2215 100 -3.0640522963959924 2216 1 -0.2905815289842586 2216 2 -0.15405615453351373 2216 3 -0.4040238203750164 2216 4 -0.37566788731146517 2216 5 -0.4518466639679133 2216 6 -0.27158457562685356 2216 7 -0.49727589152726637 2216 8 -0.37447618217508705 2216 9 -0.5731076775034225 2216 10-11 -0.48251919956172173 2216 12-13 -0.6757916911277277 2216 14-15 -0.9840558491411429 2216 16-17 -0.699983642054562 2216 18-19 -2.7563805784448903 2216 20-21 -0.9835779964598927 2216 22-23 -0.48078475884285155 2216 24-25 -0.5828842324778947 2216 26-27 -0.5873514654863641 2216 28-29 -0.53444297250104 2216 30-31 -0.6178062588455262 2216 32-33 -0.7181939975995988 2216 34-35 -0.8396912518204331 2216 36-37 -0.8183072461789394 2216 38-39 -0.799741298075979 2216 40-41 -0.8734189080254353 2216 42-43 -0.8774927603729878 2216 44-45 -0.7174217791129678 2216 46-47 -0.937862151992455 2216 48-49 -0.9496417537514361 2216 50-51 -0.777141652037038 2216 52-53 -0.7797929279806226 2216 54-55 -0.798647610704819 2216 56-57 -0.7880580959275818 2216 58-59 -0.8167707391791339 2216 60-61 -0.8405413684547582 2216 62-63 -0.8543741144091115 2216 64-65 -0.8582132175916897 2216 66-67 -0.8448048993100201 2216 68-69 -0.9712541401622588 2216 70-71 -0.9590538078025048 2216 72-73 -0.9488283220396667 2216 74-75 -1.0077659764725269 2216 76-77 -0.814767142399571 2216 78-79 -0.9943580257174567 2216 80-81 -0.9492070074354579 2216 82-83 -1.078928565415076 2216 84-85 -0.9825740080534615 2216 86-87 -0.9904169458916385 2216 88-89 -0.9349938158286974 2216 90-91 -0.977269517862311 2216 92-93 -0.8364468743763851 2216 94-95 -0.7722513843915166 2216 96-97 -0.7597400607357763 2216 98-99 -0.7825863812416003 2216 100 -0.7867900881110117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 19079.0 1 3137.0 2 486.0 3 454.0 4 440.0 5 256.0 6 242.0 7 342.0 8 615.0 9 1033.0 10 1937.0 11 3861.0 12 6560.0 13 9224.0 14 11943.0 15 14748.0 16 17448.0 17 20345.0 18 23041.0 19 25942.0 20 29672.0 21 33497.0 22 38472.0 23 44544.0 24 50347.0 25 56652.0 26 64146.0 27 73822.0 28 86823.0 29 103302.0 30 122472.0 31 146723.0 32 178582.0 33 226642.0 34 313885.0 35 420865.0 36 619707.0 37 906741.0 38 683422.0 39 244911.0 40 173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.92527447659329 21.878834712331336 12.40534647160416 23.79054433947121 2 15.311882179151853 19.837563682023625 41.094438898185956 23.75611524063856 3 18.197153664591394 27.595754056376986 28.86857731221123 25.338514966820387 4 11.956816192030404 16.90827072392227 40.80017902736318 30.334734056684148 5 12.876438675038779 38.77485697739678 35.29159865670607 13.05710569085837 6 28.94677310315385 39.15890800579492 18.533177342056103 13.361141548995128 7 26.292722562278076 31.77278314831841 24.603718684230394 17.330775605173116 8 26.005504544718644 35.08911610906277 20.13345924459353 18.77192010162505 9 26.15300180360262 14.69806295478773 22.230007888715644 36.918927352894 10-11 25.696304957623862 25.90344689109771 27.565809468051615 20.834438683226818 12-13 25.82992420567753 23.80873863568037 27.535533806153484 22.825803352488613 14-15 23.249701663612736 24.793707044579794 25.034864109630657 26.921727182176813 16-17 22.05123683375709 27.28800795226285 26.5233807244054 24.137374489574658 18-19 21.361772264697823 28.29283149619978 28.14718267692425 22.198213562178143 20-21 23.297189720032677 27.31779295455979 26.799503884658204 22.585513440749335 22-23 23.99630039250789 26.915037198421064 26.484241707544044 22.604420701526998 24-25 23.28676075026816 27.010581404357616 27.365713718288248 22.33694412708597 26-27 23.151348053053482 27.228907565738908 26.19073101222978 23.42901336897783 28-29 22.121547865506884 27.873013275289743 26.901503187215138 23.10393567198824 30-31 23.050385543304447 27.42820488872751 26.790469826893936 22.73093974107411 32-33 23.64415416072921 27.738183825823953 25.623962081750314 22.993699931696522 34-35 23.52664192814261 27.42789735094821 26.865731578829628 22.17972914207955 36-37 23.15810084835049 26.971557823925934 26.528757004509757 23.34158432321382 38-39 23.401193770079924 26.96376575058733 27.224486201187354 22.410554278145394 40-41 22.82680727230818 25.991285488649137 27.939204911069375 23.242702327973312 42-43 22.804096915679363 26.37949608273282 27.098330188947966 23.71807681263985 44-45 22.97977163024952 27.53131854410573 26.880946310318816 22.607963515325938 46-47 22.98474579342022 27.064183535079046 26.416871330163932 23.5341993413368 48-49 23.7798262038504 26.585235183463613 26.720437851073026 22.91450076161296 50-51 23.381597742221373 26.402329368222926 26.555395776410425 23.66067711314528 52-53 22.55332452366117 27.20501693006568 26.457813419000303 23.783845127272844 54-55 22.639447660507432 27.106061689114235 26.382832207726448 23.87165844265189 56-57 23.948860933199363 26.386176686514606 26.109932145716048 23.555030234569983 58-59 22.651628393318155 26.78829937478025 27.25404411130819 23.306028120593403 60-61 22.697019491595054 27.63888690352977 26.318067188699118 23.34602641617606 62-63 23.259561348531165 26.70414768828965 25.78573317784012 24.250557785339062 64-65 22.229289868509042 26.970247355252567 26.362116950303715 24.438345825934675 66-67 23.21453649942051 26.354427183446845 26.36256766502508 24.068468652107573 68-69 22.35149481217286 26.590633326060637 26.707538571036118 24.35033329073039 70-71 22.449732572001274 26.17144070641325 26.85802766339997 24.520799058185506 72-73 23.16997417738571 26.36999434482346 27.32071224231133 23.1393192354795 74-75 23.388266302146864 26.718445444962942 26.776163748431138 23.117124504459063 76-77 22.954799546164494 26.72701803055555 26.32278668443658 23.995395738843374 78-79 22.73944980524514 26.652774888356774 26.46622804710092 24.141547259297162 80-81 22.312378670176024 27.001310553062126 27.281666333845116 23.404644442916737 82-83 23.3778840759712 25.761693083171757 28.406147995936777 22.45427484492027 84-85 23.644157126224126 26.52881983560832 26.653272015207524 23.17375102296003 86-87 22.0710989392203 27.289776447325597 27.292447471410462 23.34667714204364 88-89 23.340058285356765 25.793685204190837 27.003946061347804 23.86231044910459 90-91 22.92505974529309 25.80560932955102 27.33385298805489 23.935477937101 92-93 23.316197350590265 26.133070624768074 27.130959275891957 23.419772748749704 94-95 21.877915865458228 26.16783069872547 27.93453028998706 24.019723145829246 96-97 22.336552442296615 26.093462448899317 27.35064244623014 24.21934266257393 98-99 21.62218875384106 26.934658332959273 27.18345793250935 24.25969498069032 100 22.590645917666112 26.086042316389847 26.196267998095742 25.127043767848296 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16023.0 1 9367.0 2 2492.0 3 1751.0 4 897.0 5 480.0 6 372.0 7 330.5 8 308.5 9 300.0 10 296.0 11 292.5 12 289.5 13 294.0 14 307.5 15 414.5 16 473.0 17 527.5 18 713.5 19 867.5 20 1024.0 21 1267.5 22 1735.0 23 2449.5 24 3384.5 25 4465.0 26 6337.5 27 8541.5 28 12685.0 29 22833.0 30 36872.0 31 49759.0 32 67032.5 33 82965.5 34 94871.5 35 109529.0 36 122556.0 37 146353.5 38 155585.0 39 141623.0 40 127408.0 41 112419.5 42 108706.0 43 116662.0 44 130163.0 45 161529.5 46 190040.0 47 207779.0 48 220304.5 49 215246.5 50 204806.5 51 211827.0 52 217340.0 53 208638.5 54 197034.0 55 169537.0 56 147620.0 57 120929.0 58 93409.5 59 89945.0 60 75622.5 61 52014.0 62 38396.0 63 31912.0 64 23162.5 65 10883.0 66 5810.5 67 3982.5 68 2893.5 69 2199.0 70 1685.5 71 1285.0 72 1041.0 73 920.0 74 717.5 75 533.5 76 427.5 77 286.0 78 183.5 79 127.5 80 82.5 81 64.0 82 54.0 83 42.5 84 34.0 85 19.5 86 17.5 87 18.5 88 15.5 89 12.5 90 11.0 91 11.0 92 9.5 93 9.0 94 5.5 95 3.5 96 3.5 97 3.0 98 3.5 99 5.5 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0032924978503357 2 0.4528134282333373 3 0.4861139603254769 4 0.47235089817005543 5 0.43839911708002527 6 0.4887840812168283 7 0.5057816800617732 8 0.508213009653898 9 0.5218241137098117 10-11 0.5351204474167448 12-13 0.5338830743207527 14-15 0.5309198913803505 16-17 0.5360321960137917 18-19 0.534490906718784 20-21 0.5325914304749364 22-23 0.4950252174466133 24-25 0.5305074336816864 26-27 0.510090777597816 28-29 0.5160171434786204 30-31 0.5190237430188821 32-33 0.5219326552094602 34-35 0.5342521154195574 36-37 0.5528344201593693 38-39 0.5426532274923462 40-41 0.5472445329274749 42-43 0.5445852661860883 44-45 0.554451688504131 46-47 0.5521723170115139 48-49 0.5243205682017257 50-51 0.5397008987019088 52-53 0.5307245166809833 54-55 0.5411987713970572 56-57 0.5288358945871006 58-59 0.5345451774686082 60-61 0.52921578983587 62-63 0.5237127358036944 64-65 0.5344800525688191 66-67 0.5317339526277137 68-69 0.5529646699589474 70-71 0.5370416319605222 72-73 0.5403195852499049 74-75 0.5383115675064087 76-77 0.5265565230944834 78-79 0.5567310599967481 80-81 0.5318099316774677 82-83 0.5308656206305263 84-85 0.5395272323024713 86-87 0.8465477182080319 88-89 1.1148948677888555 90-91 1.105907631617965 92-93 1.1052672367700394 94-95 1.110911394751758 96-97 1.1032375107266137 98-99 1.09968820368811 100 1.0946627322543874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4606533.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.627446583209608 #Duplication Level Percentage of deduplicated Percentage of total 1 74.04934644979629 17.49596977764144 2 11.890620242092051 5.618899892225217 3 4.996963520252322 3.541964659590264 4 2.5677296458025727 2.4267558018529605 5 1.5295267289644243 1.8069405543099128 6 0.98654031427862 1.3985657146680548 7 0.6574305729630353 1.0873384021397103 8 0.47047078059514824 0.8892818589978233 9 0.3472387151960781 0.7383927775425901 >10 1.8816261567957644 8.239751856694836 >50 0.22479023298910641 3.7844531671572423 >100 0.28536753484363403 15.247249845385305 >500 0.05819720436804865 9.628422273049315 >1k 0.050658230026526 21.25594761856563 >5k 0.002758161344456951 4.211154458705088 >10k+ 7.355096918551869E-4 2.628911341474723 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 19532 0.4240065142266429 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 16660 0.3616602768285823 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 16048 0.348374797271614 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 14750 0.32019742396288053 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 14479 0.3143144746819354 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 14301 0.3104503972944512 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 13277 0.28822109816645186 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 11974 0.2599351833580699 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 8767 0.19031666548356432 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 8578 0.18621379679685351 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 8362 0.18152480401204116 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 8276 0.1796578902180881 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 7910 0.17171265244382272 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 7341 0.15936062978383092 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 7296 0.15838375628699503 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 7235 0.1570595499912841 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 7068 0.15343426390302642 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 6611 0.14351357083515953 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 6536 0.141885448340433 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 6534 0.14184203174057367 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 6515 0.14142957404190962 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 6311 0.13700108085625348 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 6262 0.13593737415969886 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 6141 0.13331066986820675 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 6004 0.13033663277783963 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 5989 0.13001100827889434 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 5863 0.1272757624877538 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 5790 0.12569105659288668 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 5716 0.12408464239808985 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 5666 0.12299922740160549 No Hit GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT 5636 0.12234797840371489 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 5607 0.12171843770575398 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 5572 0.12095864720821493 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 5408 0.11739848601974631 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 5379 0.11676894532178539 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 5269 0.11438103232951985 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 5179 0.11242728533584802 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 5038 0.1093664150457622 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 4951 0.10747779295187943 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 4950 0.10745608465194975 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 4870 0.10571942065757481 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 4811 0.10443863096172327 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 4799 0.10417813136256703 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 4738 0.10285392506685614 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 4682 0.10163826027079366 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.1708299929686816E-5 2 0.0 0.0 0.0 0.0 2.1708299929686816E-5 3 0.0 0.0 0.0 0.0 2.1708299929686816E-5 4 0.0 0.0 0.0 0.0 2.1708299929686816E-5 5 0.0 0.0 0.0 0.0 2.1708299929686816E-5 6 0.0 0.0 0.0 0.0 6.512489978906045E-5 7 0.0 0.0 0.0 0.0 6.512489978906045E-5 8 0.0 0.0 0.0 0.0 6.512489978906045E-5 9 0.0 0.0 0.0 0.0 6.512489978906045E-5 10-11 0.0 2.1708299929686816E-5 0.0 0.0 6.512489978906045E-5 12-13 0.0 2.1708299929686816E-5 0.0 0.0 1.411039495429643E-4 14-15 0.0 2.1708299929686816E-5 0.0 0.0 1.6281224947265111E-4 16-17 0.0 2.1708299929686816E-5 0.0 0.0 2.0622884933202477E-4 18-19 0.0 2.1708299929686816E-5 0.0 4.341659985937363E-5 2.38791299226555E-4 20-21 0.0 2.1708299929686816E-5 0.0 4.341659985937363E-5 2.604995991562418E-4 22-23 0.0 2.1708299929686816E-5 0.0 6.512489978906045E-5 2.822078990859286E-4 24-25 0.0 2.1708299929686816E-5 0.0 1.5195809950780773E-4 2.822078990859286E-4 26-27 0.0 2.1708299929686816E-5 0.0 3.798952487695193E-4 3.147703489804588E-4 28-29 0.0 2.1708299929686816E-5 0.0 0.0014653102452538602 3.2562449894530223E-4 30-31 0.0 2.1708299929686816E-5 0.0 0.005926365880804502 3.2562449894530223E-4 32-33 0.0 2.1708299929686816E-5 0.0 0.017442618993503356 3.5818694883983247E-4 34-35 0.0 2.1708299929686816E-5 0.0 0.035536486984897316 3.907493987343627E-4 36-37 0.0 2.1708299929686816E-5 0.0 0.0680121036797088 3.907493987343627E-4 38-39 0.0 2.1708299929686816E-5 0.0 0.11941735791320718 4.341659985937363E-4 40-41 0.0 2.1708299929686816E-5 0.0 0.17661872822793195 4.341659985937363E-4 42-43 0.0 2.1708299929686816E-5 0.0 0.23850909132746906 4.341659985937363E-4 44-45 0.0 2.1708299929686816E-5 0.0 0.313163934785662 4.4502014855857974E-4 46-47 0.0 2.1708299929686816E-5 0.0 0.4103954101707293 4.775825984531099E-4 48-49 0.0 2.1708299929686816E-5 0.0 0.5278807293901944 5.861240981015441E-4 50-51 0.0 2.1708299929686816E-5 0.0 0.654678909279495 5.861240981015441E-4 52-53 0.0 2.1708299929686816E-5 0.0 0.7815205057686551 6.078323980312309E-4 54-55 0.0 2.1708299929686816E-5 0.0 0.9239920782071896 6.078323980312309E-4 56-57 0.0 2.1708299929686816E-5 0.0 1.0942177121058287 6.295406979609176E-4 58-59 0.0 2.1708299929686816E-5 0.0 1.303539994177834 6.512489978906045E-4 60-61 0.0 2.1708299929686816E-5 0.0 1.5269943795040652 6.621031478554479E-4 62-63 0.0 2.1708299929686816E-5 0.0 1.7609013112464407 7.163738976796649E-4 64-65 0.0 2.1708299929686816E-5 0.0 2.0115561963845696 7.163738976796649E-4 66-67 0.0 2.1708299929686816E-5 0.0 2.2937423871705684 7.163738976796649E-4 68-69 0.0 2.1708299929686816E-5 0.0 2.611378231741746 7.272280476445084E-4 70-71 0.0 2.1708299929686816E-5 0.0 2.9642575012487695 7.489363475741952E-4 72-73 0.0 2.1708299929686816E-5 0.0 3.3345793897492975 8.032070973984123E-4 74-75 0.0 2.1708299929686816E-5 0.0 3.7007115763633953 8.466236972577858E-4 76-77 0.0 2.1708299929686816E-5 0.0 4.122156511198336 8.466236972577858E-4 78-79 0.0 2.1708299929686816E-5 0.0 4.581069971711914 8.683319971874726E-4 80-81 0.0 2.1708299929686816E-5 0.0 5.078830434949667 8.791861471523161E-4 82-83 0.0 2.1708299929686816E-5 0.0 5.598559697716265 8.900402971171595E-4 84-85 0.0 2.1708299929686816E-5 0.0 6.105112022425542 9.117485970468463E-4 86-87 0.0 2.1708299929686816E-5 0.0 6.650164017060119 9.334568969765331E-4 88 0.0 2.1708299929686816E-5 0.0 7.083830724755472 9.5516519690622E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5120 0.0 41.485855 94 GTACTAG 5720 0.0 35.86567 1 TAGGCAT 6725 0.0 33.80638 5 GCATCGT 5865 0.0 33.220963 8 CAACGCA 9050 0.0 31.972584 5 GTATCAA 10720 0.0 30.927605 1 TATTCTC 2880 0.0 30.49964 5 CTAGGCA 7625 0.0 30.43282 4 CTGTGCG 7675 0.0 30.09834 9 TAGTACT 7750 0.0 29.760132 4 ATCAACG 9870 0.0 29.461649 3 AGGGCTA 6475 0.0 28.800255 5 CGTTAAC 1185 0.0 28.654964 1 ACTGTGC 8325 0.0 28.36716 8 GTACTGT 8400 0.0 27.552855 6 TACTGTG 9130 0.0 27.510956 7 GGTATCA 7070 0.0 27.48296 1 GCTATTG 6715 0.0 26.848331 8 CTAACGC 2900 0.0 26.566198 3 CTAGTAC 9040 0.0 26.45044 3 >>END_MODULE