##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146422_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4606533
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.821103854026443	31.0	31.0	34.0	30.0	34.0
2	31.82563177122578	31.0	31.0	34.0	30.0	34.0
3	31.998008046398454	33.0	31.0	34.0	30.0	34.0
4	35.73208983849676	37.0	35.0	37.0	35.0	37.0
5	35.64818139802754	37.0	35.0	37.0	33.0	37.0
6	35.676166869964895	37.0	35.0	37.0	33.0	37.0
7	35.598574459360215	37.0	35.0	37.0	33.0	37.0
8	35.581542561401385	37.0	35.0	37.0	33.0	37.0
9	37.17395056108357	39.0	37.0	39.0	33.0	39.0
10-11	37.11855011133102	39.0	37.0	39.0	33.0	39.0
12-13	37.04998781078959	39.0	37.0	39.0	33.0	39.0
14-15	38.23603260847149	40.0	37.5	41.0	33.0	41.0
16-17	38.23792502951786	40.0	37.0	41.0	33.0	41.0
18-19	38.28934450268781	40.0	38.0	41.0	33.0	41.0
20-21	38.30537022094491	40.0	38.0	41.0	33.0	41.0
22-23	38.26928473105478	40.0	37.5	41.0	33.0	41.0
24-25	38.19158996581594	40.0	37.0	41.0	33.0	41.0
26-27	38.04286607737316	40.0	37.0	41.0	33.0	41.0
28-29	37.97812801948884	40.0	37.0	41.0	33.0	41.0
30-31	37.851011053215075	40.0	37.0	41.0	33.0	41.0
32-33	37.725389137557464	40.0	36.0	41.0	32.5	41.0
34-35	37.62309897703979	40.0	36.0	41.0	32.0	41.0
36-37	37.5415553302234	40.0	36.0	41.0	32.0	41.0
38-39	37.41864054810853	40.0	36.0	41.0	31.5	41.0
40-41	37.28431968250309	39.5	36.0	41.0	31.0	41.0
42-43	37.15413717865475	39.0	35.0	41.0	31.0	41.0
44-45	36.934310684412765	39.0	35.0	41.0	31.0	41.0
46-47	36.679531873536995	39.0	35.0	41.0	30.0	41.0
48-49	36.3959867431754	39.0	35.0	41.0	30.0	41.0
50-51	36.18419025761891	38.5	35.0	41.0	29.0	41.0
52-53	35.97581695387833	38.0	35.0	40.0	29.0	41.0
54-55	35.768050722745286	38.0	34.5	40.0	29.0	41.0
56-57	35.48765427274699	37.0	34.0	40.0	28.0	41.0
58-59	35.179041700124586	37.0	34.0	40.0	28.0	41.0
60-61	34.91596825638718	36.0	34.0	39.5	27.5	41.0
62-63	34.63518529010864	36.0	34.0	39.0	27.0	41.0
64-65	34.359264548848344	35.0	33.0	39.0	26.5	41.0
66-67	34.1389327939255	35.0	33.0	39.0	26.0	41.0
68-69	33.95783651175407	35.0	33.0	38.0	26.0	41.0
70-71	33.77808180251829	35.0	33.0	37.0	26.0	40.0
72-73	33.59802990665648	35.0	33.0	37.0	26.0	40.0
74-75	33.42599184679671	35.0	33.0	36.5	26.0	39.5
76-77	33.28697341362799	35.0	33.0	36.0	26.0	39.0
78-79	33.18592865827728	35.0	33.0	36.0	27.0	39.0
80-81	33.05811149078928	35.0	33.0	35.5	27.0	38.0
82-83	32.87237560221537	35.0	33.0	35.0	26.0	37.0
84-85	32.7383164301656	35.0	33.0	35.0	26.0	37.0
86-87	32.6100031194827	35.0	33.0	35.0	26.0	36.5
88-89	32.505336117205715	35.0	33.0	35.0	26.0	36.0
90-91	32.417779596933315	35.0	33.0	35.0	26.0	36.0
92-93	32.333334961455826	35.0	33.0	35.0	26.0	36.0
94-95	32.29307529111373	35.0	33.0	35.0	26.0	35.0
96-97	32.241301647030426	35.0	33.0	35.0	25.5	35.0
98-99	32.19825908117884	35.0	33.0	35.0	25.0	35.0
100	31.687720895519472	34.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4720130051602922
1101	2	0.6114148053773896
1101	3	0.48717399894505675
1101	4	0.3258605903387135
1101	5	0.37464435822602127
1101	6	0.37931464823601146
1101	7	0.40181907837551734
1101	8	0.45011456509131875
1101	9	0.4538799373198401
1101	10-11	0.5251714147546735
1101	12-13	0.4703489039524982
1101	14-15	0.6394562624212696
1101	16-17	0.6560325378213037
1101	18-19	0.6769312484213046
1101	20-21	0.6218353181447043
1101	22-23	0.6435955408664995
1101	24-25	0.6938875033333076
1101	26-27	0.7409939515844783
1101	28-29	0.7381585532566461
1101	30-31	0.8098901083856092
1101	32-33	0.7376720544561977
1101	34-35	0.7463462136519894
1101	36-37	0.7835261736168064
1101	38-39	0.7672668190262115
1101	40-41	0.75440632337137
1101	42-43	0.7973601749084125
1101	44-45	0.7710711601767102
1101	46-47	0.7924986574145265
1101	48-49	0.8326908985238788
1101	50-51	0.8686548764715312
1101	52-53	0.8777717042600344
1101	54-55	0.7390984123090121
1101	56-57	0.801317843715637
1101	58-59	0.7912165816799259
1101	60-61	0.8158603914721354
1101	62-63	0.7542004458552398
1101	64-65	0.7329627135737056
1101	66-67	0.7369900565770067
1101	68-69	0.7686924609743215
1101	70-71	0.7451227541399632
1101	72-73	0.658243095539575
1101	74-75	0.6344582104980674
1101	76-77	0.6964176863443754
1101	78-79	0.6000273019184874
1101	80-81	0.6052826444770361
1101	82-83	0.588332774999131
1101	84-85	0.6327672877583908
1101	86-87	0.5720446828807795
1101	88-89	0.5857963634271144
1101	90-91	0.5932593457157651
1101	92-93	0.582475477063042
1101	94-95	0.5859748407293282
1101	96-97	0.6389115235456302
1101	98-99	0.6221537195423181
1101	100	0.7216033882681891
1102	1	0.4550824457820539
1102	2	0.5472491283783931
1102	3	0.45031867611430343
1102	4	0.3588186064857979
1102	5	0.3024700527021267
1102	6	0.3632912474957877
1102	7	0.39081330316734153
1102	8	0.4086068382474295
1102	9	0.43300320008947324
1102	10-11	0.4850351822966701
1102	12-13	0.4898956813254216
1102	14-15	0.6182701535955957
1102	16-17	0.5931023688954653
1102	18-19	0.6166167805453213
1102	20-21	0.66852386602541
1102	22-23	0.6171832789193417
1102	24-25	0.6139689467975487
1102	26-27	0.6345671205415542
1102	28-29	0.6665177049684772
1102	30-31	0.7296669023466933
1102	32-33	0.7397241564105173
1102	34-35	0.6963709902209771
1102	36-37	0.683209123402591
1102	38-39	0.6869910834400699
1102	40-41	0.633771412930173
1102	42-43	0.7084437562775037
1102	44-45	0.6420465036383334
1102	46-47	0.6589255922440742
1102	48-49	0.5938543269918313
1102	50-51	0.5678948462954736
1102	52-53	0.5690431485480048
1102	54-55	0.581398279555593
1102	56-57	0.615035990644202
1102	58-59	0.5536057559168412
1102	60-61	0.5990188678870112
1102	62-63	0.5360164960893954
1102	64-65	0.5598995572115939
1102	66-67	0.5744894847067954
1102	68-69	0.5602916124928896
1102	70-71	0.5601282129779008
1102	72-73	0.5600970346482086
1102	74-75	0.5140213495723458
1102	76-77	0.5145318028291044
1102	78-79	0.44026394295576665
1102	80-81	0.4384792425903825
1102	82-83	0.39835069051734706
1102	84-85	0.4484742407239466
1102	86-87	0.49141057529465826
1102	88-89	0.477399574950816
1102	90-91	0.4677263929983013
1102	92-93	0.4408717535651405
1102	94-95	0.4708251721794383
1102	96-97	0.5080202687151569
1102	98-99	0.4224686999889755
1102	100	0.49712091316499496
1103	1	0.26773631927638064
1103	2	-0.19598697458280157
1103	3	0.17845892922769124
1103	4	0.1799136197140001
1103	5	0.20522905757859178
1103	6	0.25035161440438713
1103	7	0.3401220588361511
1103	8	0.3271123242571008
1103	9	0.3450317387760151
1103	10-11	0.4061908081264036
1103	12-13	0.4365869509292395
1103	14-15	0.48323669377855083
1103	16-17	0.4993080188474508
1103	18-19	0.5426589956218919
1103	20-21	0.5102373264734723
1103	22-23	0.46874032162202894
1103	24-25	0.41772336568408974
1103	26-27	0.5041729377618225
1103	28-29	0.5321675997313946
1103	30-31	0.5292900301301842
1103	32-33	0.5707815054644385
1103	34-35	0.5455576405155327
1103	36-37	0.5432860146013923
1103	38-39	0.4825468964462587
1103	40-41	0.5835679781485297
1103	42-43	0.5501615083051874
1103	44-45	0.6357794562820587
1103	46-47	0.6597737852049335
1103	48-49	0.6305811487564483
1103	50-51	0.6292426788629939
1103	52-53	0.6317768732389553
1103	54-55	0.5702640345344534
1103	56-57	0.5751075312763803
1103	58-59	0.546056867374368
1103	60-61	0.5927307500669414
1103	62-63	0.5075396500157652
1103	64-65	0.5402197638877126
1103	66-67	0.5986778804424162
1103	68-69	0.5554708175326084
1103	70-71	0.5079166865023694
1103	72-73	0.4142921173006471
1103	74-75	0.46643218217098337
1103	76-77	0.470917413019599
1103	78-79	0.448904998773358
1103	80-81	0.4316006252705833
1103	82-83	0.38514323504218595
1103	84-85	0.40430820411813073
1103	86-87	0.4004716257565235
1103	88-89	0.43161972559400397
1103	90-91	0.4097023391714032
1103	92-93	0.45984206533130845
1103	94-95	0.4748325339868913
1103	96-97	0.45799703060627905
1103	98-99	0.44291218586585046
1103	100	0.45866331323646037
1104	1	0.4120790509337482
1104	2	0.5001220571493583
1104	3	0.42994706719609965
1104	4	0.25621039914766186
1104	5	0.2995225736863034
1104	6	0.265539226345588
1104	7	0.3019982427098995
1104	8	0.29884816323718155
1104	9	0.3284325322803028
1104	10-11	0.33326190960342217
1104	12-13	0.384319900461378
1104	14-15	0.5277948447975263
1104	16-17	0.505309473729028
1104	18-19	0.5106851795835325
1104	20-21	0.49844099700217726
1104	22-23	0.4676683036164988
1104	24-25	0.526181593607852
1104	26-27	0.5377939461893178
1104	28-29	0.500963183204739
1104	30-31	0.5682059636424057
1104	32-33	0.5579965284841109
1104	34-35	0.5503887634954125
1104	36-37	0.5628139223281465
1104	38-39	0.5918244719509573
1104	40-41	0.5775547150511287
1104	42-43	0.5290823780227711
1104	44-45	0.5068559201931677
1104	46-47	0.5177037297658984
1104	48-49	0.5398329688722967
1104	50-51	0.6060784169116431
1104	52-53	0.5558889276399626
1104	54-55	0.5482908689735027
1104	56-57	0.5278746289185108
1104	58-59	0.5734682974538075
1104	60-61	0.5143789699368995
1104	62-63	0.5089125577688876
1104	64-65	0.5128774250561818
1104	66-67	0.47367063826538214
1104	68-69	0.4641558042212637
1104	70-71	0.4575300528948034
1104	72-73	0.4449539126143591
1104	74-75	0.43176579991725816
1104	76-77	0.3916881525537974
1104	78-79	0.3969284558087409
1104	80-81	0.42148319163636216
1104	82-83	0.4114063088076847
1104	84-85	0.4072405288794201
1104	86-87	0.402377856607238
1104	88-89	0.38384106683933084
1104	90-91	0.3976060478106689
1104	92-93	0.35395665132002563
1104	94-95	0.3392666762757983
1104	96-97	0.3554362300151013
1104	98-99	0.3150507641175935
1104	100	0.3538749987015315
1105	1	0.16964156204666736
1105	2	-0.377012075410061
1105	3	0.07067175384944235
1105	4	0.13154384323348012
1105	5	0.16657576275510877
1105	6	0.25806746446192363
1105	7	0.2991456504990424
1105	8	0.24731070026768975
1105	9	0.36604134471230765
1105	10-11	0.34732124995559843
1105	12-13	0.34865012293072084
1105	14-15	0.42327790456469216
1105	16-17	0.42059516239852
1105	18-19	0.48218544817714104
1105	20-21	0.47499508226200504
1105	22-23	0.43654850370931797
1105	24-25	0.45525929099590456
1105	26-27	0.43715293793486154
1105	28-29	0.49537567329728205
1105	30-31	0.45659368812337675
1105	32-33	0.46248424550623923
1105	34-35	0.44331293232439606
1105	36-37	0.5087480818599488
1105	38-39	0.4952452639592977
1105	40-41	0.550779671051508
1105	42-43	0.5233715617995571
1105	44-45	0.5170644739013426
1105	46-47	0.4496857791991715
1105	48-49	0.4815527891971101
1105	50-51	0.4948197488345727
1105	52-53	0.43518818650242963
1105	54-55	0.5032045404258767
1105	56-57	0.5007760294477066
1105	58-59	0.49560494077957884
1105	60-61	0.47571873407177634
1105	62-63	0.4845174772887475
1105	64-65	0.5015192304271494
1105	66-67	0.5469165204578914
1105	68-69	0.5092726085374437
1105	70-71	0.43347740101322074
1105	72-73	0.3915732683814781
1105	74-75	0.4239614415433124
1105	76-77	0.3813446333823194
1105	78-79	0.34408675049171933
1105	80-81	0.37461074384071225
1105	82-83	0.38548462399871397
1105	84-85	0.3647969467221728
1105	86-87	0.36249216710898935
1105	88-89	0.35319437592766434
1105	90-91	0.25118274089217607
1105	92-93	0.33298332345651005
1105	94-95	0.4017084370443982
1105	96-97	0.3575451889869612
1105	98-99	0.3795630877800491
1105	100	0.33018607831976254
1106	1	0.2831293519279967
1106	2	0.329812705419652
1106	3	0.26722097582434046
1106	4	0.1942870703602111
1106	5	0.20135215068275159
1106	6	0.24405163092092153
1106	7	0.2620816000981705
1106	8	0.2379118640469855
1106	9	0.29623557417842505
1106	10-11	0.28947468703265145
1106	12-13	0.2663028912133143
1106	14-15	0.3223316230075781
1106	16-17	0.3324282988220304
1106	18-19	0.3328902939539091
1106	20-21	0.31408216047724125
1106	22-23	0.3228342216477671
1106	24-25	0.2901942545625573
1106	26-27	0.3401399533511551
1106	28-29	0.3357363987933226
1106	30-31	0.4185194597826083
1106	32-33	0.44533387634557187
1106	34-35	0.4026435144765159
1106	36-37	0.39460795904976464
1106	38-39	0.46123528001277947
1106	40-41	0.4146854574699148
1106	42-43	0.4126505724091558
1106	44-45	0.43430378058165076
1106	46-47	0.4930437816978568
1106	48-49	0.4236838910131695
1106	50-51	0.5095878724328031
1106	52-53	0.4550383126584663
1106	54-55	0.4668962967780317
1106	56-57	0.4265281818541027
1106	58-59	0.4010122631549464
1106	60-61	0.4150149548060824
1106	62-63	0.47555425879668434
1106	64-65	0.4611866705531966
1106	66-67	0.4570609357865649
1106	68-69	0.4488680218308474
1106	70-71	0.4738843677591902
1106	72-73	0.4483124681089805
1106	74-75	0.4302200664047575
1106	76-77	0.37460621996159205
1106	78-79	0.36224606250609526
1106	80-81	0.3510408869131041
1106	82-83	0.3556738576826035
1106	84-85	0.3345671562018424
1106	86-87	0.3489688501147441
1106	88-89	0.3190412539767209
1106	90-91	0.3515865461946248
1106	92-93	0.437335972733365
1106	94-95	0.4117354890147169
1106	96-97	0.36382079392652145
1106	98-99	0.37294440229492665
1106	100	0.3916190204180161
1107	1	0.02235164256914146
1107	2	-0.5122714516899407
1107	3	-0.07476092742101415
1107	4	0.04803041511752326
1107	5	0.09085428294266507
1107	6	0.15898094886953373
1107	7	0.1561585685005511
1107	8	0.11926818828237629
1107	9	0.1875196764039302
1107	10-11	0.22483154139577266
1107	12-13	0.17918218239111638
1107	14-15	0.24045113172953592
1107	16-17	0.29684531009142034
1107	18-19	0.2941812931090908
1107	20-21	0.16395780633432366
1107	22-23	0.19551079388591575
1107	24-25	0.24561728442640174
1107	26-27	0.30964208212414945
1107	28-29	0.23712646050808672
1107	30-31	0.2758412926669891
1107	32-33	0.29222412004838816
1107	34-35	0.3820510177928256
1107	36-37	0.29787745976827296
1107	38-39	0.28612760544425697
1107	40-41	0.2923188480087191
1107	42-43	0.2450311119132138
1107	44-45	0.26637678362237693
1107	46-47	0.202899773736533
1107	48-49	0.2548207341139346
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2216	86-87	-0.5755286678954619
2216	88-89	-0.5673850187987952
2216	90-91	-0.6447704879339184
2216	92-93	-0.6356262731828721
2216	94-95	-0.6171442159303879
2216	96-97	-0.6651589358217329
2216	98-99	-0.6591308270783394
2216	100	-0.6910270315820881
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	12.0
13	61.0
14	293.0
15	929.0
16	2148.0
17	4473.0
18	7813.0
19	12204.0
20	17403.0
21	24629.0
22	33101.0
23	39192.0
24	41156.0
25	42386.0
26	46749.0
27	57008.0
28	71844.0
29	90028.0
30	110723.0
31	137256.0
32	169697.0
33	215300.0
34	297906.0
35	408702.0
36	611211.0
37	1032223.0
38	802696.0
39	328774.0
40	613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.72909077211236	22.346939152134755	13.646462350330458	24.27750772542243
2	14.885865902964959	20.383945070442106	40.3413038041515	24.388885222441438
3	17.880090534082488	27.31602829255204	29.73151512599095	25.072366047374523
4	12.21155558257803	17.48287812525786	39.42252487394739	30.883041418216717
5	12.516360350824101	38.95149607796612	35.178884385262194	13.353259185947586
6	28.86363779441068	39.4889171530954	17.974363800281033	13.67308125221289
7	25.467186452569752	32.491785541859	24.56229355312653	17.478734452444712
8	26.176280512915028	34.87766179033125	19.78448868161804	19.16156901513568
9	26.198531520342954	14.178971473774313	22.074475532900774	37.54802147298196
10-11	25.698024957164094	26.0278174496959	27.268370811627747	21.005786781512256
12-13	25.9787892543047	23.8600808894672	27.87522633616214	22.285903520065958
14-15	23.28868090529311	24.639369362474916	25.71738872036356	26.354561011868416
16-17	21.58284813671473	27.005392520808098	27.209316209257594	24.202443133219578
18-19	20.95656321142169	27.212287418759402	29.91844408799416	21.91270528182475
20-21	22.65585780882163	26.385834197776397	28.88164964934021	22.076658344061762
22-23	23.205027246839926	25.945862339011367	28.635563136634723	22.21354727751399
24-25	22.54631628602248	26.08126328412279	29.5600183478551	21.81240208199963
26-27	22.526364804668166	26.28699104747184	28.331169733334495	22.8554744145255
28-29	21.532484408556282	26.947674096766484	29.040658126187306	22.479183368489924
30-31	22.548541386765276	26.468452521668684	28.770248688113163	22.21275740345288
32-33	23.216505775601735	26.767234707750927	27.794091565174938	22.2221679514724
34-35	22.942605523579044	26.514488421376203	28.8337950715792	21.70911098346556
36-37	22.723445159298763	26.031117111285212	28.490732618218516	22.75470511119751
38-39	22.886539616670497	25.942612372471878	29.32956303580155	21.841284975056077
40-41	22.279595064859762	25.15860415535343	29.842849763557727	22.718951016229077
42-43	22.50633154032404	25.512137573384052	28.75259022492449	23.228940661367417
44-45	22.659655319955377	26.606788662970608	28.39804903166872	22.335506985405292
46-47	22.810202379967752	26.255776307257538	27.646713916952294	23.287307395822413
48-49	23.803022657393846	25.960968928860346	27.597045007722226	22.638963406023585
50-51	23.107171923005872	26.03929028620874	27.215999538047374	23.637538252738015
52-53	22.19474192356181	26.771828919040512	27.185361223436498	23.848067933961172
54-55	22.458744593936743	26.719186457014438	26.881315440568716	23.940753508480103
56-57	23.71457731899904	25.974934058275323	26.711236734726217	23.599251887999415
58-59	22.34101997124946	26.428018795959296	27.865252787243826	23.365708445547423
60-61	22.551402308397243	27.200574749385627	26.857974099590592	23.390048842626538
62-63	23.06270247059991	26.31123015942792	26.29578470402795	24.330282665944214
64-65	22.07240239025749	26.534044149906233	26.8705770695662	24.52297639027008
66-67	22.893529689247856	25.97599973776374	26.911909672632323	24.21856090035608
68-69	22.25303399744359	25.92923769728798	27.370125979493164	24.447602325775257
70-71	22.318195366240335	25.56680271364553	27.44617005069145	24.668831869422686
72-73	22.9684775947551	25.696483667869092	27.83186400705259	23.503174730323217
74-75	23.13065122422026	26.238081034636963	27.20418910223848	23.4270786389043
76-77	22.755180048973173	26.277779424038233	26.636269824186982	24.330770702801612
78-79	22.40279760630039	26.298903757823577	26.954997053625963	24.343301582250074
80-81	22.007922920851982	26.750701526707942	27.6398809304135	23.60149462202657
82-83	23.06994957284866	25.444067846650576	28.788315593341245	22.69766698715952
84-85	23.469900144759272	26.225905657247907	26.885148671332193	23.41904552666063
86-87	21.821478570179476	27.038114935207247	27.463390811466276	23.677015683147
88-89	22.88373639988915	25.621772465237637	27.25667862852982	24.2378125063434
90-91	22.433031207598372	25.54921031207598	27.643516960651286	24.374241519674356
92-93	22.9231037435347	26.042936713873345	27.069840438650424	23.96411910394153
94-95	21.44276111014387	26.01125244384741	27.918006992779283	24.62797945322944
96-97	21.944969599843343	25.93531240905814	27.3561030486777	24.763614942420812
98-99	21.2304239947734	26.79432355140465	27.246712290509162	24.72854016331279
100	22.178016477213475	25.960258282059307	26.294625786491466	25.56709945423576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	109.0
2	85.0
3	105.0
4	65.0
5	55.5
6	130.0
7	353.5
8	597.5
9	655.5
10	568.5
11	476.0
12	474.5
13	567.0
14	728.5
15	1052.0
16	1455.0
17	1998.5
18	2825.0
19	3766.5
20	4876.0
21	5910.5
22	6925.0
23	8052.0
24	9160.5
25	10089.5
26	11598.0
27	13290.5
28	16804.5
29	26152.0
30	39222.0
31	50721.0
32	67219.5
33	82478.0
34	92890.0
35	107102.0
36	119182.5
37	143141.0
38	153458.5
39	139179.0
40	125245.5
41	110738.0
42	106587.5
43	113388.5
44	126013.0
45	158723.0
46	189756.5
47	208271.0
48	221262.5
49	215715.0
50	204245.5
51	209416.0
52	213567.0
53	206609.0
54	199109.0
55	171449.0
56	148090.0
57	121651.5
58	93546.5
59	92013.5
60	77753.0
61	52359.0
62	37430.5
63	30656.0
64	21785.0
65	9305.0
66	4772.5
67	3313.0
68	2375.5
69	1875.5
70	1485.5
71	1066.0
72	839.5
73	752.0
74	602.5
75	456.5
76	322.5
77	183.0
78	89.5
79	47.5
80	25.0
81	16.5
82	11.5
83	6.0
84	3.5
85	1.5
86	0.5
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013806478755280814
2	0.004276535086148303
3	0.0019754552936015004
4	0.02850299780767879
5	0.0026484125914217915
6	0.0
7	4.5587429852342315E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.00869417412183957
16-17	0.0033213698892420825
18-19	0.0
20-21	5.427074982421704E-5
22-23	7.70644647503882E-4
24-25	0.0
26-27	2.822078990859286E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.861240981015441E-4
36-37	0.0
38-39	0.0
40-41	3.798952487695193E-4
42-43	6.838114477851347E-4
44-45	0.0
46-47	0.0
48-49	5.101450483476402E-4
50-51	0.0
52-53	1.0854149964843408E-5
54-55	3.364786489101457E-4
56-57	7.597904975390386E-5
58-59	2.3879129922655496E-4
60-61	7.597904975390386E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0014978726951483902
70-71	7.814987974687253E-4
72-73	0.0
74-75	1.9537469936718133E-4
76-77	0.0028980580406131897
78-79	5.101450483476402E-4
80-81	0.0032779532893827093
82-83	0.001866913793953066
84-85	0.005752699481367006
86-87	0.007521925925636481
88-89	0.007956091924230219
90-91	0.006143448880101369
92-93	0.00879186147152316
94-95	0.0014544560952890167
96-97	0.00487351333421469
98-99	0.002094850943214778
100	0.08277374763189582
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4606533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.0182854098228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.63602535680585	11.17012612688488
2	15.477828733486115	5.268122138216457
3	6.625309057243217	3.3825420139334725
4	3.410166270194049	2.321407315244529
5	2.026275904746254	1.7241870833009332
6	1.298687629770671	1.326086204498614
7	0.8600159321854777	1.0245197613649089
8	0.6593870158993478	0.8977309145685172
9	0.47406671153504093	0.7261022340179514
>10	2.609583463222679	8.309576076756787
>50	0.3249332050124236	3.9546758788176235
>100	0.42019107962808006	16.123209041722887
>500	0.09169393184719732	10.915468803672306
>1k	0.07997748500005544	24.66746664331533
>5k	0.004966754641723188	5.673794962206516
>10k+	8.914687818477515E-4	2.51498480147832
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	21760	0.47237260646998513	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	17546	0.3808938305662849	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	16197	0.35160933396113736	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	16196	0.3515876256612077	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	15826	0.3435555546872236	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	15319	0.33254944662287234	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	13197	0.2864844341720769	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9059	0.19665548906303287	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	8976	0.19485370016886885	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	8841	0.19192307967836114	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8831	0.1917059966790643	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8267	0.17946251551872092	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8139	0.176683853127721	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	7881	0.1710831117458618	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7783	0.16895569835275248	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7652	0.16611191106196352	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7634	0.16572116166322914	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7284	0.15812325668783878	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7253	0.15745029939001848	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7092	0.15395526310133892	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7054	0.1531303477040108	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6986	0.1516541833087921	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6962	0.1511331841104796	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	6932	0.15048193511258903	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6921	0.15024314381336246	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6864	0.1490057707173703	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6848	0.1486584379184953	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6816	0.14796377232074534	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6459	0.14021390924584715	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6406	0.13906336934957375	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	6212	0.1348519591632145	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6030	0.1309010485760115	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6030	0.1309010485760115	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5979	0.1297939252795975	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	5734	0.12447539179682421	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5693	0.12358535149970705	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	5652	0.12269531120258988	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5448	0.11826681801693378	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	5439	0.1180714433175666	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	5404	0.11731165282002755	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	5352	0.11618282122368385	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5349	0.11611769632389479	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	5266	0.11431590742973079	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	5247	0.11390344973106672	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	5007	0.1086934577479419	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	5006	0.10867174944801221	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	4881	0.10595821195680136	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4852	0.10532867125884043	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	4838	0.10502475505982482	No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA	4803	0.10426496456228578	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	4799	0.10417813136256703	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	4796	0.10411300646277798	No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT	4794	0.10406958986291859	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	4788	0.10393934006334048	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	4758	0.10328809106544988	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.341659985937363E-5
2	0.0	0.0	0.0	0.0	4.341659985937363E-5
3	0.0	0.0	0.0	0.0	4.341659985937363E-5
4	0.0	0.0	0.0	0.0	4.341659985937363E-5
5	0.0	0.0	0.0	0.0	4.341659985937363E-5
6	0.0	0.0	0.0	0.0	8.683319971874726E-5
7	0.0	0.0	0.0	0.0	8.683319971874726E-5
8	0.0	0.0	0.0	0.0	8.683319971874726E-5
9	0.0	0.0	0.0	0.0	8.683319971874726E-5
10-11	0.0	0.0	0.0	0.0	8.683319971874726E-5
12-13	0.0	0.0	0.0	0.0	3.147703489804588E-4
14-15	0.0	0.0	0.0	0.0	3.798952487695193E-4
16-17	0.0	0.0	0.0	0.0	3.907493987343627E-4
18-19	0.0	0.0	0.0	4.341659985937363E-5	3.907493987343627E-4
20-21	0.0	0.0	0.0	6.512489978906045E-5	4.4502014855857974E-4
22-23	0.0	0.0	0.0	8.683319971874726E-5	4.5587429852342315E-4
24-25	0.0	0.0	0.0	1.5195809950780773E-4	4.5587429852342315E-4
26-27	0.0	0.0	0.0	4.4502014855857974E-4	5.427074982421704E-4
28-29	0.0	0.0	0.0	0.0015847058948671375	5.427074982421704E-4
30-31	0.0	0.0	0.0	0.006165157180031055	5.427074982421704E-4
32-33	0.0	0.0	0.0	0.017768243492448658	5.535616482070138E-4
34-35	0.0	0.0	0.0	0.03637225653219026	5.644157981718572E-4
36-37	0.0	0.0	0.0	0.069661934474365	5.644157981718572E-4
38-39	0.0	0.0	0.0	0.12191381240512117	5.752699481367007E-4
40-41	0.0	0.0	0.0	0.18002693131689276	5.861240981015441E-4
42-43	0.0	2.1708299929686816E-5	0.0	0.24333918806182436	5.861240981015441E-4
44-45	0.0	2.1708299929686816E-5	0.0	0.3194919042151657	5.861240981015441E-4
46-47	0.0	2.1708299929686816E-5	0.0	0.4184166269947485	5.969782480663875E-4
48-49	0.0	2.1708299929686816E-5	0.0	0.5379208181076744	7.380821976093518E-4
50-51	0.0	2.1708299929686816E-5	0.0	0.6668029947902252	8.032070973984123E-4
52-53	0.0	2.1708299929686816E-5	0.0	0.7957502963725648	8.24915397328099E-4
54-55	0.0	2.1708299929686816E-5	0.0	0.9399585328054743	8.466236972577858E-4
56-57	0.0	2.1708299929686816E-5	0.0	1.1122681634973635	9.117485970468463E-4
58-59	0.0	2.1708299929686816E-5	0.0	1.324749003209138	9.5516519690622E-4
60-61	0.0	2.1708299929686816E-5	0.0	1.5515464667245409	9.768734968359067E-4
62-63	0.0	2.1708299929686816E-5	0.0	1.788112665208303	0.0010202900966952803
64-65	0.0	2.1708299929686816E-5	0.0	2.042772731683459	0.0010311442466601236
66-67	0.0	2.1708299929686816E-5	0.0	2.3292354575556065	0.0010528525465898107
68-69	0.0	2.1708299929686816E-5	0.0	2.650540004814901	0.001063706696554654
70-71	0.0	2.1708299929686816E-5	0.0	3.00829278765614	0.001063706696554654
72-73	0.0	2.1708299929686816E-5	0.0	3.3840851677389483	0.0011071232964140277
74-75	0.0	2.1708299929686816E-5	0.0	3.7567189901819873	0.0011831023461679317
76-77	0.0	2.1708299929686816E-5	0.0	4.187954368285215	0.001193956496132775
78-79	0.0	2.1708299929686816E-5	0.0	4.657884791013111	0.001193956496132775
80-81	0.0	2.1708299929686816E-5	0.0	5.167150653213599	0.001193956496132775
82-83	0.0	2.1708299929686816E-5	0.0	5.698548127192403	0.001193956496132775
84-85	0.0	2.1708299929686816E-5	0.0	6.217007454413112	0.001193956496132775
86-87	0.0	2.1708299929686816E-5	0.0	6.774074993058771	0.0012699355458866788
88	0.0	2.1708299929686816E-5	0.0	7.217988018320937	0.0013459145956405826
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10235	0.0	41.390865	1
GGGCACC	5160	0.0	40.936832	94
GTATCAA	17195	0.0	37.188126	1
GTGGTAT	2205	0.0	33.264694	1
TATTCTC	2685	0.0	32.73157	5
CAACGCA	18790	0.0	32.665245	5
ATCAACG	19340	0.0	31.746288	3
GTACTAG	5305	0.0	31.463753	1
CAGTACG	725	0.0	31.119265	9
TAGTACT	7700	0.0	29.732748	4
CTGTGCG	7650	0.0	29.492113	9
TATCAAC	21775	0.0	29.276367	2
AGGGCTA	6490	0.0	29.182995	5
ACTGTGC	8035	0.0	28.776278	8
AACGCAG	22415	0.0	28.577038	6
TAGGCAT	6900	0.0	27.78949	5
GTACTGT	8330	0.0	27.644655	6
CTATTGA	6895	0.0	27.336077	9
GCTATTG	6965	0.0	27.191885	8
ACGCAGA	22840	0.0	26.851868	7
>>END_MODULE