FastQCFastQC Report
Mon 16 Jan 2017
ERR1146421_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146421_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4045227
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC143680.3551840230474087No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125120.3093027906715742No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT123140.3044081333383763No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA112790.27882242455120565No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA103500.2558570878717066No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC101820.25170404528596296No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC99270.24540031993260208No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC90280.2231765980005572No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG65880.16285859854094714No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT64780.16013934446694833No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC60980.1507455576658615No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT59300.14659251508011786No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT56860.14056071513415688No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC56500.13967077743721182No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG55840.13803922499281251No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC55520.13724816926219469No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT55120.13625934959892239No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG51050.12619810952512678No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA50560.12498680543761821No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT49460.1222675513636194No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA48490.11986966368018408No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC48310.11942469483171156No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA47590.11764481943782142No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG46280.11440643504060463No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC45460.11237935473089643No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC45250.11186022440767848No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT44800.11074780228649715No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA44140.10911624984209786No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA43440.10738581543137135No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA43050.10642171625968086No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC41150.10172482285913745No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT40900.10110681056959227No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT40460.10001910893999275No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC42100.036.90718594
TACCCCG2450.034.5166745
GTACTAG45050.031.6321961
GCATCGT45750.029.9633438
GTATCAA104750.029.5056021
CAACGCA94550.029.4159345
TAGGCAT58400.028.2368835
TATTCTC20800.027.7820515
ATCAACG102700.027.2204973
CAGTACG7750.026.6545359
CTAGGCA65950.025.9320054
AGGGCTA45850.025.4117685
TATCAAC121600.025.346922
CTGTGCG64050.025.2883269
AACGCAG118350.025.227016
TAGTACT66150.024.930264
ACGCAGA112200.024.5605667
GTGATCG27600.024.3234678
CTATTGA47850.024.234569
CGCAGAG112850.024.2114038
Produced by FastQC (version 0.11.5)