##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146421_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4045227
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.663425810220293	31.0	31.0	34.0	27.0	34.0
2	30.716740988824608	31.0	31.0	34.0	27.0	34.0
3	30.892341764751396	31.0	31.0	34.0	27.0	34.0
4	34.35765236413185	37.0	35.0	37.0	30.0	37.0
5	34.555056613633795	37.0	35.0	37.0	30.0	37.0
6	34.71382298199829	37.0	35.0	37.0	31.0	37.0
7	34.56976209246107	37.0	35.0	37.0	30.0	37.0
8	34.53991333490061	37.0	35.0	37.0	30.0	37.0
9	35.933393849096724	39.0	35.0	39.0	30.0	39.0
10-11	35.86557713572069	39.0	35.0	39.0	30.0	39.0
12-13	35.879039791833684	39.0	35.0	39.0	30.0	39.0
14-15	36.928300686216126	40.0	36.0	41.0	30.0	41.0
16-17	36.75502326569065	40.0	36.0	41.0	29.5	41.0
18-19	36.71022899332967	40.0	36.0	41.0	29.5	41.0
20-21	37.04339163166863	40.0	36.0	41.0	30.0	41.0
22-23	36.752966273586125	40.0	36.0	41.0	29.5	41.0
24-25	36.85756077471054	40.0	36.0	41.0	30.0	41.0
26-27	36.926243323304234	40.0	36.0	41.0	30.0	41.0
28-29	36.76203461511554	40.0	36.0	41.0	29.5	41.0
30-31	36.70944226368508	40.0	36.0	41.0	30.0	41.0
32-33	36.813538770506575	40.0	36.0	41.0	30.0	41.0
34-35	36.86003121703677	40.0	36.0	41.0	30.0	41.0
36-37	36.788456494530465	40.0	35.0	41.0	30.0	41.0
38-39	36.61541935718317	39.5	35.0	41.0	30.0	41.0
40-41	36.580903000004696	39.0	35.0	41.0	30.0	41.0
42-43	36.41769473011033	39.0	35.0	41.0	29.0	41.0
44-45	36.2252041727201	39.0	35.0	41.0	28.5	41.0
46-47	36.09446861696513	39.0	35.0	41.0	28.0	41.0
48-49	35.95191011530379	38.5	34.5	40.0	28.0	41.0
50-51	35.73211466253933	38.0	34.0	40.0	27.5	41.0
52-53	35.536537751775114	38.0	34.0	40.0	27.5	41.0
54-55	35.3311401313202	38.0	34.0	40.0	27.0	41.0
56-57	35.1701287715127	37.0	34.0	40.0	27.0	41.0
58-59	34.89217749708483	37.0	33.0	40.0	27.0	41.0
60-61	34.630916015343516	36.0	33.0	40.0	26.5	41.0
62-63	34.354539683434325	36.0	33.0	39.0	26.0	41.0
64-65	34.06239217230578	35.0	33.0	39.0	26.0	41.0
66-67	33.769846908467684	35.0	32.5	38.5	26.0	40.5
68-69	33.48070788116464	35.0	32.0	38.0	26.0	40.0
70-71	33.424599039806665	35.0	32.0	37.0	26.0	40.0
72-73	33.22461780265978	35.0	32.0	37.0	26.0	40.0
74-75	32.966733510875905	35.0	32.0	36.0	25.0	39.0
76-77	32.74497216101841	35.0	32.0	36.0	25.0	39.0
78-79	32.537003609438976	35.0	32.0	36.0	25.0	39.0
80-81	32.32994341232272	35.0	32.0	35.0	24.5	37.5
82-83	32.10375709447207	35.0	31.0	35.0	24.0	37.0
84-85	31.92699890512943	35.0	31.0	35.0	24.0	37.0
86-87	31.770712372877963	35.0	31.0	35.0	24.0	36.0
88-89	31.78300748017355	35.0	32.0	35.0	24.0	36.0
90-91	31.716609475809392	35.0	32.0	35.0	24.0	36.0
92-93	31.57500283667641	35.0	32.0	35.0	24.0	36.0
94-95	31.558450737127977	35.0	32.0	35.0	24.0	35.0
96-97	31.471420886887188	35.0	31.5	35.0	24.0	35.0
98-99	31.39937078932777	35.0	31.0	35.0	23.5	35.0
100	30.911286313475117	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7269014663624809
1101	2	0.8406627995072995
1101	3	0.7812584802872955
1101	4	0.8412232098865715
1101	5	0.7314112640667716
1101	6	0.5306613562207829
1101	7	0.5788839414779332
1101	8	0.6800345295789967
1101	9	0.8170077906480344
1101	10-11	0.8019658572552331
1101	12-13	0.7053166653756691
1101	14-15	0.9355892151578331
1101	16-17	1.0015046358066328
1101	18-19	1.2293870223051329
1101	20-21	0.8473619843369349
1101	22-23	1.1181736138427638
1101	24-25	1.0087265559767715
1101	26-27	0.9455023901322193
1101	28-29	1.0832419135291644
1101	30-31	1.1028967540427672
1101	32-33	0.9276726625826868
1101	34-35	0.8722914863813713
1101	36-37	0.8011877837465207
1101	38-39	0.9350000815598918
1101	40-41	0.8911292658620411
1101	42-43	0.8453597237027424
1101	44-45	0.8023306651057993
1101	46-47	0.8643487630367375
1101	48-49	0.786597087833357
1101	50-51	0.8691777509319394
1101	52-53	0.8126637119670264
1101	54-55	0.8838286297886668
1101	56-57	0.7383596887411841
1101	58-59	0.6556463650628075
1101	60-61	0.7346813667206646
1101	62-63	0.6300103774527344
1101	64-65	0.6540548672244526
1101	66-67	0.7009834227651268
1101	68-69	0.6882824864419064
1101	70-71	0.6360185299828984
1101	72-73	0.5412910958103865
1101	74-75	0.5336064736061559
1101	76-77	0.6177266320555077
1101	78-79	0.5702813854316489
1101	80-81	0.6064702426659352
1101	82-83	0.5420738059861421
1101	84-85	0.6193289541689388
1101	86-87	0.6323540460089383
1101	88-89	0.6431149544510006
1101	90-91	0.7320078860070431
1101	92-93	0.7396996149033299
1101	94-95	0.7155950888222726
1101	96-97	0.6444461400564947
1101	98-99	0.6849885320882336
1101	100	0.7436455946989042
1102	1	0.5747813322676905
1102	2	0.7165358485806301
1102	3	0.7180801964633368
1102	4	0.808965991871986
1102	5	0.5911629025023473
1102	6	0.4197426670771023
1102	7	0.48339109343123
1102	8	0.4530809161611984
1102	9	0.6178180718649813
1102	10-11	0.6542626812286656
1102	12-13	0.5956966680258233
1102	14-15	0.7701978395653128
1102	16-17	0.8550991885813204
1102	18-19	0.8924498096139715
1102	20-21	0.6845656811498841
1102	22-23	0.9794133165631038
1102	24-25	0.8663968303988412
1102	26-27	0.7417662389285198
1102	28-29	0.8859649869084265
1102	30-31	0.9391700314994154
1102	32-33	0.8333928962894319
1102	34-35	0.6946395540789752
1102	36-37	0.686570480849916
1102	38-39	0.645671344204942
1102	40-41	0.5851445366131856
1102	42-43	0.6078714770271816
1102	44-45	0.6884763653054193
1102	46-47	0.6249820067347187
1102	48-49	0.5241225060225148
1102	50-51	0.6072096309939141
1102	52-53	0.6045612079552001
1102	54-55	0.6776258212400705
1102	56-57	0.5817567760353057
1102	58-59	0.5163654499193981
1102	60-61	0.5646516169908793
1102	62-63	0.5329411827764687
1102	64-65	0.4521288772488248
1102	66-67	0.5053597377315029
1102	68-69	0.5072946756167909
1102	70-71	0.46205459444023234
1102	72-73	0.38542241679738254
1102	74-75	0.438826480817049
1102	76-77	0.40211280999188403
1102	78-79	0.36718290383982577
1102	80-81	0.50866775321456
1102	82-83	0.49795377127218643
1102	84-85	0.4723869756275043
1102	86-87	0.446883119525328
1102	88-89	0.47513187692702274
1102	90-91	0.43547601230082833
1102	92-93	0.4917901754959786
1102	94-95	0.4850732508469342
1102	96-97	0.5242554694515817
1102	98-99	0.5280148431641081
1102	100	0.5732139640905309
1103	1	0.4512301358907784
1103	2	0.6366185462351552
1103	3	0.5862980069766444
1103	4	0.5820789982419114
1103	5	0.4991229895024816
1103	6	0.32923488696881265
1103	7	0.3401804432940878
1103	8	0.43597105660462887
1103	9	0.5061224572670682
1103	10-11	0.6037238707391239
1103	12-13	0.46465396716945406
1103	14-15	0.614941810778717
1103	16-17	0.8776900190473782
1103	18-19	0.9374729416938337
1103	20-21	0.6384125228924447
1103	22-23	0.8814549930791955
1103	24-25	0.7811933568972407
1103	26-27	0.6116153019510833
1103	28-29	0.8405550694434893
1103	30-31	0.8556437744938208
1103	32-33	0.6850793055827822
1103	34-35	0.609926363102673
1103	36-37	0.5655801351632235
1103	38-39	0.677631323643098
1103	40-41	0.6223128298421585
1103	42-43	0.7006067907517206
1103	44-45	0.6415993524267378
1103	46-47	0.6150791126920296
1103	48-49	0.5661867455405343
1103	50-51	0.5967282786146058
1103	52-53	0.6407317387235523
1103	54-55	0.6495887273647227
1103	56-57	0.5877108320600897
1103	58-59	0.5194222113781919
1103	60-61	0.49024780429381565
1103	62-63	0.4992971078783057
1103	64-65	0.553179607732261
1103	66-67	0.6042314000268973
1103	68-69	0.5718768911184711
1103	70-71	0.4473877051452746
1103	72-73	0.46497325796961064
1103	74-75	0.45823078754879276
1103	76-77	0.4678960300724526
1103	78-79	0.5212845173946334
1103	80-81	0.47720274650443884
1103	82-83	0.3574127845285844
1103	84-85	0.41490822849946696
1103	86-87	0.4127253110882201
1103	88-89	0.4696349593282534
1103	90-91	0.5086015884140629
1103	92-93	0.5727793455051966
1103	94-95	0.4963181682305766
1103	96-97	0.4462624009458551
1103	98-99	0.4903384446394625
1103	100	0.5829527010376978
1104	1	0.4025986891328941
1104	2	0.5637023230007223
1104	3	0.5387210887541372
1104	4	0.6361528522076298
1104	5	0.5012115086591251
1104	6	0.2634860690191232
1104	7	0.2677780880060965
1104	8	0.2966872174038002
1104	9	0.4335786237571071
1104	10-11	0.48588947895638057
1104	12-13	0.42078986038575295
1104	14-15	0.537542814373225
1104	16-17	0.6956195433818166
1104	18-19	0.5896215902617428
1104	20-21	0.4566595213696516
1104	22-23	0.730783837384628
1104	24-25	0.600956263482594
1104	26-27	0.36376634505307237
1104	28-29	0.6560352374734961
1104	30-31	0.7127136386896638
1104	32-33	0.4890924793477254
1104	34-35	0.36328223492965606
1104	36-37	0.3943504107521463
1104	38-39	0.5335094286956448
1104	40-41	0.44536530346587
1104	42-43	0.41266952922490674
1104	44-45	0.41320642833562005
1104	46-47	0.36863828653015673
1104	48-49	0.4603167156223975
1104	50-51	0.5054024993127939
1104	52-53	0.5035193815460772
1104	54-55	0.4298444355978859
1104	56-57	0.4222990086069842
1104	58-59	0.4917308017528583
1104	60-61	0.40043380217878166
1104	62-63	0.3577582290467731
1104	64-65	0.310176104897657
1104	66-67	0.43213796297336415
1104	68-69	0.40829338545336924
1104	70-71	0.29691200971283394
1104	72-73	0.34444937965712086
1104	74-75	0.3525253410060074
1104	76-77	0.3570275853078684
1104	78-79	0.3369825506839774
1104	80-81	0.35050251061716864
1104	82-83	0.3135659015864789
1104	84-85	0.26465732987236734
1104	86-87	0.28586452939280704
1104	88-89	0.2147660500333899
1104	90-91	0.2647041728678303
1104	92-93	0.22250676070788344
1104	94-95	0.16592796466623483
1104	96-97	0.23214075992170535
1104	98-99	0.25405896153021246
1104	100	0.2902018658914258
1105	1	0.3090474937039076
1105	2	0.48710040739631566
1105	3	0.4369278737585809
1105	4	0.5413177301472132
1105	5	0.38860788443056293
1105	6	0.08159515169035814
1105	7	0.2087521950908453
1105	8	0.2238030255029244
1105	9	0.2099736127465306
1105	10-11	0.2629482897067632
1105	12-13	0.26199735219128684
1105	14-15	0.4246708095613343
1105	16-17	0.5599382286066117
1105	18-19	0.5584717859775239
1105	20-21	0.43411767563359405
1105	22-23	0.6669850032041396
1105	24-25	0.5161507705670445
1105	26-27	0.3606620658757578
1105	28-29	0.5588825167630986
1105	30-31	0.5313438150734555
1105	32-33	0.45558258878120483
1105	34-35	0.27542404697958034
1105	36-37	0.19476635595602687
1105	38-39	0.3684072947749897
1105	40-41	0.27798794677681116
1105	42-43	0.30972081155126574
1105	44-45	0.3924792358575715
1105	46-47	0.2734895471858181
1105	48-49	0.31160421380756276
1105	50-51	0.3408586274016727
1105	52-53	0.31620113295377905
1105	54-55	0.314072454316225
1105	56-57	0.31974407819998163
1105	58-59	0.3325740084877964
1105	60-61	0.32603791328297405
1105	62-63	0.315794774758011
1105	64-65	0.21834888817416243
1105	66-67	0.29927768394338017
1105	68-69	0.2495596357993506
1105	70-71	0.2255335026169334
1105	72-73	0.21315671769364286
1105	74-75	0.2625017365671525
1105	76-77	0.3104019347397511
1105	78-79	0.30189518663546266
1105	80-81	0.42784414908688007
1105	82-83	0.3424517475771438
1105	84-85	0.3008064600761067
1105	86-87	0.3042607131317645
1105	88-89	0.3248757329402139
1105	90-91	0.29780914579375306
1105	92-93	0.344407555014282
1105	94-95	0.25265266483452464
1105	96-97	0.3062308093852657
1105	98-99	0.26055693195792884
1105	100	0.4346876640706583
1106	1	0.45592798747385643
1106	2	0.5348262599216547
1106	3	0.49017166314084903
1106	4	0.6224214813771525
1106	5	0.5187359781847292
1106	6	0.2872518429034514
1106	7	0.31808494613261473
1106	8	0.40089832448111196
1106	9	0.565624733659277
1106	10-11	0.5103606380696988
1106	12-13	0.4110967713216098
1106	14-15	0.6139575858107804
1106	16-17	0.6841022716811125
1106	18-19	0.6546490994577283
1106	20-21	0.5170317744778785
1106	22-23	0.8934456085350391
1106	24-25	0.6449977397670708
1106	26-27	0.4602119490953669
1106	28-29	0.677502752920681
1106	30-31	0.5969463465812765
1106	32-33	0.4728468464124518
1106	34-35	0.472065356237934
1106	36-37	0.4060849168468863
1106	38-39	0.5441159852539244
1106	40-41	0.4254768099608839
1106	42-43	0.4544435152316808
1106	44-45	0.5472283911177414
1106	46-47	0.5196106856786287
1106	48-49	0.4617485874435374
1106	50-51	0.5590084425665509
1106	52-53	0.4972837161518484
1106	54-55	0.5308774938929659
1106	56-57	0.47485610446508986
1106	58-59	0.4717733875707353
1106	60-61	0.48074588127035867
1106	62-63	0.45865937585103467
1106	64-65	0.4868283707231029
1106	66-67	0.5477958994489072
1106	68-69	0.5043967804322236
1106	70-71	0.5143857464594745
1106	72-73	0.473984695865191
1106	74-75	0.5169679508476577
1106	76-77	0.4397675518893962
1106	78-79	0.4852678489905742
1106	80-81	0.5178131157013226
1106	82-83	0.5001247539372642
1106	84-85	0.47232607096191614
1106	86-87	0.4573884479110184
1106	88-89	0.4865516746838665
1106	90-91	0.4374249615996355
1106	92-93	0.43315846926041957
1106	94-95	0.4022582644156891
1106	96-97	0.4285472581927827
1106	98-99	0.4446161082285016
1106	100	0.3828544831228129
1107	1	0.3387661769765451
1107	2	0.5158346483759289
1107	3	0.5172554871516084
1107	4	0.6736949771592862
1107	5	0.4396345447779382
1107	6	0.2669739158222484
1107	7	0.21393762087153334
1107	8	0.25546474463205726
1107	9	0.3887879525056448
1107	10-11	0.43395359222429164
1107	12-13	0.28014970898436786
1107	14-15	0.4981097250012141
1107	16-17	0.6495766189460426
1107	18-19	0.7103343056932374
1107	20-21	0.43253683328229187
1107	22-23	0.7467093565950549
1107	24-25	0.4339848305778844
1107	26-27	0.35243072126819186
1107	28-29	0.5801807169767059
1107	30-31	0.6067937086371131
1107	32-33	0.5455490227877888
1107	34-35	0.3750787162539524
1107	36-37	0.3666177028408555
1107	38-39	0.4713015523494306
1107	40-41	0.3287923564732296
1107	42-43	0.4227327945242223
1107	44-45	0.4494869717067047
1107	46-47	0.4047256915404347
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2216	98-99	0.21375629678151853
2216	100	0.3161104974962292
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16730.0
1	2723.0
2	411.0
3	397.0
4	387.0
5	201.0
6	199.0
7	360.0
8	645.0
9	1093.0
10	1716.0
11	2968.0
12	4637.0
13	6258.0
14	7985.0
15	10123.0
16	12361.0
17	14598.0
18	16686.0
19	18797.0
20	21564.0
21	24807.0
22	29208.0
23	33982.0
24	39190.0
25	44091.0
26	50359.0
27	58502.0
28	70023.0
29	83380.0
30	100655.0
31	120951.0
32	150892.0
33	193532.0
34	269552.0
35	368686.0
36	550989.0
37	840066.0
38	654648.0
39	220732.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.104692817190944	21.546105074340954	12.742868714127589	23.606333394340513
2	15.285623009859107	20.049197437621043	40.97757682505682	23.68760272746303
3	18.448957225076278	27.394462463605286	28.74495144017629	25.41162887114215
4	12.171398710679858	17.189296000206642	40.60695306966084	30.032352219452658
5	13.187194889714625	38.672305036048385	34.859950313359526	13.280549760877456
6	29.04707891897048	38.860105686355176	18.686691031366074	13.406124363308273
7	26.580159374368765	31.87872481622356	24.046284308310618	17.494831501097057
8	26.04983951830147	34.78889910896506	20.171746972116086	18.98951440061738
9	26.053307946940052	15.212975090968323	21.959652631837905	36.77406433025372
10-11	25.549928454842053	25.87103977513136	27.583923433322582	20.995108336704003
12-13	25.83907916857359	23.716996111674508	27.531471876152153	22.912452843599745
14-15	23.495378467392054	24.80579746522434	24.9962256085779	26.702598458805703
16-17	22.262667870219136	27.385785124131264	26.380651469165763	23.970895536483845
18-19	21.657608847605587	28.18210907283431	27.91069137451653	22.24959070504357
20-21	23.5178144588362	27.123005034602016	26.725217297081283	22.633963209480502
22-23	24.0161844027185	26.74933019413656	26.503779221751984	22.730706181392954
24-25	23.4054281077669	26.97113723852908	27.145596154945363	22.477838498758658
26-27	23.2230213646539	27.152482264657536	26.176412600165257	23.448083770523308
28-29	22.386342176147263	27.678699221739606	26.751558011708692	23.18340059040444
30-31	23.289716012589082	27.167601761358334	26.633108701046066	22.90957352500652
32-33	23.627327815777875	27.526303148798682	25.644854095319413	23.20151494010403
34-35	23.65385279719819	27.280004632567096	26.70464924334323	22.36149332689148
36-37	23.292344064270036	26.905861810578198	26.35335866209077	23.448435463061
38-39	23.49197011062558	26.819585694964985	27.021117395539857	22.66732679886957
40-41	23.083900784738315	26.001068290030055	27.63045914101329	23.28457178421834
42-43	23.03490558616388	26.28435762514309	26.94659603022326	23.734140758469767
44-45	23.138388013815298	27.302117871010235	26.71474769083192	22.844746424342546
46-47	23.137545289314705	26.986478330728325	26.24507087206042	23.630905507896546
48-49	23.85228209127172	26.464980134767487	26.585901102254507	23.096836671706285
50-51	23.458477740205446	26.34106859987645	26.492715232775655	23.70773842714245
52-53	22.850985899601543	26.99496767597291	26.36044104720255	23.793605377223
54-55	22.86057773861273	26.907039605649757	26.332268611210562	23.900114044526948
56-57	24.00334650172401	26.327656714431647	26.060689576675983	23.608307207168362
58-59	22.878501109869706	26.608764311093562	27.101411509714822	23.411323069321906
60-61	22.90264684882914	27.282844054686016	26.330463575665203	23.484045520819638
62-63	23.429784735320975	26.550851097694178	25.746042273647674	24.273321893337176
64-65	22.404775343101036	26.74120311586266	26.429833070264973	24.42418847077133
66-67	23.354379841676508	26.253688378634354	26.3082389798466	24.083692799842538
68-69	22.638707827554402	26.44079492185048	26.608755763633336	24.311741486961782
70-71	22.68716642331231	26.08037528348193	26.68735282239274	24.545105470813013
72-73	23.308528001873015	26.238454286767393	27.08323650546805	23.36978120589154
74-75	23.44980239433226	26.636756878607127	26.612288397031126	23.30115233002949
76-77	22.999398739552337	26.594946156810067	26.19805710495642	24.20759799868118
78-79	22.968142937239307	26.552834653527984	26.32167888837419	24.15734352085852
80-81	22.60138640836681	26.854041918306855	27.048519819511185	23.496051853815146
82-83	23.461954110155954	25.754371175830798	28.01300203549776	22.77067267851549
84-85	23.707514741213398	26.411156857645157	26.5114675698276	23.369860831313844
86-87	22.259881188523625	27.079627772073533	27.110529176897018	23.549961862505825
88-89	23.30661418576634	25.707262914487274	26.895132397327842	24.090990502418546
90-91	23.08631630252416	25.725951128982373	27.078617914374174	24.109114654119292
92-93	23.30854543311797	26.135310866463403	26.85524017077044	23.700903529648187
94-95	22.13688033203683	26.012009826926462	27.662383060912372	24.188726780124334
96-97	22.491117309994916	25.986793719762268	27.11598979179557	24.406099178447246
98-99	21.900739537917204	26.656281136128385	27.074830680070217	24.36814864588419
100	22.739046438452366	25.875372699758326	26.212695659562986	25.172885202226325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14085.0
1	8292.5
2	2237.5
3	1522.0
4	788.0
5	422.5
6	320.0
7	308.5
8	317.0
9	317.5
10	281.0
11	258.5
12	256.0
13	262.5
14	265.0
15	306.0
16	358.5
17	391.5
18	462.5
19	547.5
20	669.0
21	864.0
22	1180.5
23	1688.0
24	2365.0
25	3224.0
26	4626.5
27	6427.0
28	9972.0
29	17947.0
30	28681.0
31	38863.0
32	53227.0
33	67063.0
34	78443.5
35	91180.5
36	102836.5
37	122060.0
38	130461.5
39	120984.0
40	110891.5
41	102563.5
42	101127.0
43	107679.0
44	119396.0
45	143985.0
46	167569.5
47	185287.0
48	199404.5
49	196905.5
50	185974.0
51	187522.5
52	189380.0
53	182353.0
54	172593.5
55	149289.0
56	130354.0
57	107526.0
58	84296.0
59	79355.5
60	66205.5
61	46678.0
62	35309.5
63	29117.5
64	21068.5
65	10876.0
66	6228.0
67	4357.0
68	3213.5
69	2385.0
70	1860.5
71	1506.0
72	1203.5
73	1017.0
74	831.0
75	605.0
76	417.5
77	306.0
78	217.5
79	143.0
80	97.5
81	74.0
82	56.0
83	43.5
84	33.5
85	29.5
86	26.0
87	22.5
88	21.0
89	18.5
90	17.0
91	14.0
92	10.0
93	9.5
94	7.0
95	4.5
96	3.0
97	1.5
98	3.0
99	4.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9946042583024389
2	0.449616300889913
3	0.483878902222298
4	0.46768698023621424
5	0.43470984446608313
6	0.4877600194006418
7	0.5034080905719259
8	0.5060037421880157
9	0.5197236150159187
10-11	0.5325906308842495
12-13	0.5318737366283771
14-15	0.5290061596048875
16-17	0.5335423698101491
18-19	0.5324670284263404
20-21	0.5308231157361504
22-23	0.49424914844086626
24-25	0.5279555387126605
26-27	0.50838926962566
28-29	0.5158424978375751
30-31	0.5178077769183287
32-33	0.5201562236186004
34-35	0.5324793886721314
36-37	0.550364664331569
38-39	0.5386718718133742
40-41	0.5432328025102175
42-43	0.5414405668705367
44-45	0.5511557200621868
46-47	0.5488690745908696
48-49	0.521874297783536
50-51	0.5371392013353021
52-53	0.5300073395139506
54-55	0.5388696357460286
56-57	0.5271274022446701
58-59	0.5328872767832312
60-61	0.5267936756083157
62-63	0.5201562236186004
64-65	0.5329243575206039
66-67	0.5300073395139506
68-69	0.5504141053147327
70-71	0.5340120591502034
72-73	0.5380167787864563
74-75	0.5360267792141208
76-77	0.5235923719484716
78-79	0.553701930695113
80-81	0.5284375882985058
82-83	0.5289072776385603
84-85	0.5371392013353021
86-87	0.8425361543369506
88-89	1.1071690167201989
90-91	1.0973302610706397
92-93	1.0980224348349301
94-95	1.1022001979122555
96-97	1.0957975905925674
98-99	1.0913479021078423
100	1.0881218779564164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4045227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.358275929666952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.06550439257535	20.263045060420364
2	12.356480925165673	6.761040293406978
3	4.9232172095460465	4.0407220464133715
4	2.5760218402433144	2.819020652249002
5	1.4710136758219656	2.0122199019725495
6	0.92517750513934	1.518675688171376
7	0.6470999431916681	1.2392477159936626
8	0.43657460201667886	0.9555142740685468
9	0.33244904168194583	0.8185709353399224
>10	1.701415292802726	8.757740641992934
>50	0.20946723094436123	4.07288765724282
>100	0.2678105422768537	16.317181906543528
>500	0.051212962891047695	9.91101340582996
>1k	0.03483567263789976	16.720772308379726
>5k	0.0011762694656953165	2.034662180557989
>10k+	5.428935995516845E-4	1.7576853314173784
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	14368	0.3551840230474087	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12512	0.3093027906715742	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	12314	0.3044081333383763	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	11279	0.27882242455120565	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10350	0.2558570878717066	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10182	0.25170404528596296	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	9927	0.24540031993260208	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9028	0.2231765980005572	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6588	0.16285859854094714	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6478	0.16013934446694833	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	6098	0.1507455576658615	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5930	0.14659251508011786	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5686	0.14056071513415688	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5650	0.13967077743721182	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5584	0.13803922499281251	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5552	0.13724816926219469	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5512	0.13625934959892239	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5105	0.12619810952512678	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5056	0.12498680543761821	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4946	0.1222675513636194	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4849	0.11986966368018408	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4831	0.11942469483171156	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4759	0.11764481943782142	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4628	0.11440643504060463	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4546	0.11237935473089643	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4525	0.11186022440767848	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4480	0.11074780228649715	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4414	0.10911624984209786	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4344	0.10738581543137135	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	4305	0.10642171625968086	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4115	0.10172482285913745	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4090	0.10110681056959227	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	4046	0.10001910893999275	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.47204915818074E-5	0.0
10-11	0.0	0.0	0.0	4.94409831636148E-5	0.0
12-13	0.0	0.0	0.0	4.94409831636148E-5	0.0
14-15	0.0	0.0	0.0	7.41614747454222E-5	1.23602457909037E-5
16-17	0.0	0.0	0.0	7.41614747454222E-5	2.47204915818074E-5
18-19	0.0	0.0	0.0	7.41614747454222E-5	2.47204915818074E-5
20-21	0.0	0.0	0.0	8.65217205363259E-5	8.65217205363259E-5
22-23	0.0	0.0	0.0	1.977639326544592E-4	9.88819663272296E-5
24-25	0.0	0.0	0.0	3.337266363543999E-4	9.88819663272296E-5
26-27	0.0	0.0	0.0	6.674532727087998E-4	1.23602457909037E-4
28-29	0.0	0.0	0.0	0.0014708692491175403	1.483229494908444E-4
30-31	0.0	0.0	0.0	0.005809315521724739	1.483229494908444E-4
32-33	0.0	0.0	0.0	0.01650092813085644	1.483229494908444E-4
34-35	0.0	0.0	0.0	0.03328614191490366	1.730434410726518E-4
36-37	0.0	0.0	0.0	0.06307433427098158	1.730434410726518E-4
38-39	0.0	0.0	0.0	0.1106118395827972	2.1012417844536288E-4
40-41	0.0	0.0	0.0	0.16552841163178234	2.224844242362666E-4
42-43	0.0	0.0	0.0	0.22341144267058438	2.719254073998814E-4
44-45	0.0	0.0	0.0	0.29721447028807035	2.842856531907851E-4
46-47	0.0	0.0	0.0	0.3925243255817288	2.966458989816888E-4
48-49	0.0	0.0	0.0	0.5077836175819058	2.966458989816888E-4
50-51	0.0	0.0	0.0	0.630471417302416	2.966458989816888E-4
52-53	0.0	0.0	0.0	0.7558908313427157	3.70807373727111E-4
54-55	0.0	1.23602457909037E-5	0.0	0.8954009255846458	3.955278653089184E-4
56-57	0.0	2.47204915818074E-5	0.0	1.0664914478223348	4.0788811109982207E-4
58-59	0.0	2.47204915818074E-5	0.0	1.2782842594494697	4.202483568907258E-4
60-61	0.0	2.47204915818074E-5	0.0	1.503784583658717	4.449688484725332E-4
62-63	0.0	2.47204915818074E-5	0.0	1.7352178258475977	4.696893400543406E-4
64-65	0.0	2.47204915818074E-5	0.0	1.9793574007095276	4.696893400543406E-4
66-67	0.0	2.47204915818074E-5	0.0	2.258513551897088	4.696893400543406E-4
68-69	0.0	2.47204915818074E-5	0.0	2.580893482615438	4.696893400543406E-4
70-71	0.0	2.47204915818074E-5	0.0	2.9407125978344357	4.696893400543406E-4
72-73	0.0	2.47204915818074E-5	0.0	3.3208890378710514	4.820495858452443E-4
74-75	0.0	2.47204915818074E-5	0.0	3.691770573072908	5.067700774270517E-4
76-77	0.0	2.47204915818074E-5	0.0	4.123934206906064	5.31490569008859E-4
78-79	0.0	2.47204915818074E-5	0.0	4.5940067145799235	5.438508147997628E-4
80-81	0.0	2.47204915818074E-5	0.0	5.109119957915835	5.438508147997628E-4
82-83	0.0	2.47204915818074E-5	0.0	5.653675306725679	5.438508147997628E-4
84-85	0.0	2.47204915818074E-5	0.0	6.1755248840176336	5.438508147997628E-4
86-87	0.0	2.47204915818074E-5	0.0	6.740919607230942	5.562110605906665E-4
88	0.0	2.47204915818074E-5	0.0	7.191907895403645	5.685713063815702E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4210	0.0	36.907185	94
TACCCCG	245	0.0	34.516674	5
GTACTAG	4505	0.0	31.632196	1
GCATCGT	4575	0.0	29.963343	8
GTATCAA	10475	0.0	29.505602	1
CAACGCA	9455	0.0	29.415934	5
TAGGCAT	5840	0.0	28.236883	5
TATTCTC	2080	0.0	27.782051	5
ATCAACG	10270	0.0	27.220497	3
CAGTACG	775	0.0	26.654535	9
CTAGGCA	6595	0.0	25.932005	4
AGGGCTA	4585	0.0	25.411768	5
TATCAAC	12160	0.0	25.34692	2
CTGTGCG	6405	0.0	25.288326	9
AACGCAG	11835	0.0	25.22701	6
TAGTACT	6615	0.0	24.93026	4
ACGCAGA	11220	0.0	24.560566	7
GTGATCG	2760	0.0	24.323467	8
CTATTGA	4785	0.0	24.23456	9
CGCAGAG	11285	0.0	24.211403	8
>>END_MODULE