FastQCFastQC Report
Mon 16 Jan 2017
ERR1146421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4045227
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC157020.38816115881753976No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA121100.2993651530556876No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC119220.2947177006383078No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT118830.29375360146661733No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC110660.2735569598442807No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA109720.2712332336355908No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC95540.2361795765725879No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG70040.17314232303897903No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC66230.1637238157463104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65600.16216642477665655No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT64870.1603618288911846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61840.15287151994189696No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG60670.1499792224268255No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA60380.14926232817095306No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG59570.14725996835282668No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT57220.14145065283110192No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT56510.1396954979287936No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA56030.13850891433286686No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC56000.13843475285812143No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT53290.13173549963945164No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA52320.1293376119560163No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT51920.128348792292744No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC51850.12817574885167138No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT50800.12558009723558158No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG50790.12555537674399977No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC50390.12456655708072749No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC50090.12382494233327326No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT48610.12016630957916577No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT47890.11838643418527564No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT47680.11786730386205768No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA47280.11687848419878538No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC45770.11314568996993246No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC45480.11242879571406006No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA45030.11131637359287873No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG44720.11055003835384268No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA43410.10731165395662591No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG43040.10639699576809904No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA42940.10614979085228098No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT40530.1001921523810654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC41800.035.67233794
GGTATCA96100.035.3750461
GTATCAA151100.034.0746731
TACCCCG3050.033.9000935
TATTCTC22400.031.4717795
GTACTAG41650.030.255331
CAGTACG9500.029.1935529
CAACGCA169800.028.896215
ATCAACG175400.027.981943
CTGTGCG58000.027.3124399
TATCAAC194050.026.5052592
TAGGCAT54400.026.2635085
TAGTACT60950.025.9927564
TGAACCG6150.025.9825535
AACGCAG196950.025.8428126
ACTGTGC64100.025.7342228
AGGGCTA46750.025.4341355
CTAACGC19600.024.945043
TACTGTG70900.024.4594947
TCTAACG19950.024.2729592
Produced by FastQC (version 0.11.5)