FastQCFastQC Report
Mon 16 Jan 2017
ERR1146420_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146420_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922712
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA91540.31320225872408913No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90370.30919912738579786No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC88390.3024245974286895No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT79440.27180235343064935No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC78980.2702284727335434No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA71420.2443620856245843No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC69790.23878507358918705No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC59610.20395440946627655No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT45090.15427452311414877No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC45010.1540008047320434No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG42770.14633669003309255No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT41060.1404859596155899No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC40410.13826199776098363No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT39230.1342246516249292No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC37790.1292977207470322No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA37340.1277580548476894No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA36860.12611574455505709No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC35810.1225231907899239No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT35650.1219757540257131No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG34520.1181094818784745No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC34460.11790419309189548No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG34290.11732254152992154No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT32910.11260089943860359No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA32870.1124640402475509No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG31490.10774239815623297No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC31180.1066817394255746No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA31050.10623694705465335No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT30830.10548422150386354No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC30480.10428670358215246No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC27700.036.63121494
GTACTAG29650.034.1984251
CAACGCA58100.032.751475
GTATCAA64900.031.8289091
TAGGCAT37350.030.5680375
ATCAACG63000.030.1326683
GCATCGT30400.029.6573858
TATTCTC16850.028.7203145
ACCGTGT3850.028.052558
AACGCAG73850.027.337326
GGTATCA42900.027.1538871
ACGCAGA70100.027.0620757
TATCAAC76950.026.8697282
CTAGGCA44250.026.5465774
CTGTGCG38650.026.3660689
AGGGCTA38250.026.286595
CGCAGAG71100.026.2196038
GAACCGT3450.025.8566636
TAGTACT41000.024.8689614
GCTATTG37800.024.7210258
Produced by FastQC (version 0.11.5)