##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146420_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2922712
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.408494918418235	31.0	30.0	34.0	26.0	34.0
2	30.474600987028484	31.0	30.0	34.0	26.0	34.0
3	30.75373420302787	31.0	31.0	34.0	27.0	34.0
4	34.23902834080128	37.0	35.0	37.0	30.0	37.0
5	34.42905561683806	37.0	35.0	37.0	30.0	37.0
6	34.53458534402295	37.0	35.0	37.0	30.0	37.0
7	34.471734813419864	37.0	35.0	37.0	30.0	37.0
8	34.39935922526749	37.0	35.0	37.0	30.0	37.0
9	35.780861747582385	39.0	35.0	39.0	30.0	39.0
10-11	35.698905331760365	39.0	35.0	39.0	29.5	39.0
12-13	35.68556771929633	39.0	35.0	39.0	30.0	39.0
14-15	36.70516527115912	40.0	36.0	41.0	29.5	41.0
16-17	36.54004602574595	40.0	36.0	41.0	28.0	41.0
18-19	36.49801725246962	40.0	36.0	41.0	27.5	41.0
20-21	36.82558750229239	40.0	36.0	41.0	30.0	41.0
22-23	36.55518573160818	40.0	36.0	41.0	28.0	41.0
24-25	36.6669175751836	40.0	36.0	41.0	29.5	41.0
26-27	36.73450890816474	40.0	36.0	41.0	29.5	41.0
28-29	36.591723029843514	39.5	35.5	41.0	29.5	41.0
30-31	36.546628610687605	39.0	35.0	41.0	29.5	41.0
32-33	36.649032816096835	39.5	35.0	41.0	30.0	41.0
34-35	36.69986368824571	40.0	35.0	41.0	30.0	41.0
36-37	36.62963952657668	39.5	35.0	41.0	30.0	41.0
38-39	36.46261383263216	39.0	35.0	41.0	29.0	41.0
40-41	36.42476337045866	39.0	35.0	41.0	29.0	41.0
42-43	36.26870471671516	39.0	35.0	41.0	28.5	41.0
44-45	36.07597036587936	39.0	35.0	41.0	28.0	41.0
46-47	35.950906042059565	39.0	34.5	40.5	28.0	41.0
48-49	35.820573494754186	38.0	34.0	40.0	28.0	41.0
50-51	35.60970649862183	38.0	34.0	40.0	27.0	41.0
52-53	35.423037233911515	38.0	34.0	40.0	27.0	41.0
54-55	35.240338938629606	38.0	34.0	40.0	27.0	41.0
56-57	35.09616205770531	37.0	33.0	40.0	27.0	41.0
58-59	34.834927628859774	37.0	33.0	40.0	26.0	41.0
60-61	34.587728965426635	36.0	33.0	40.0	26.0	41.0
62-63	34.32409060489025	36.0	33.0	39.0	26.0	41.0
64-65	34.046914988544884	35.0	32.5	39.0	26.0	41.0
66-67	33.76498898967808	35.0	32.0	39.0	26.0	41.0
68-69	33.48371324304276	35.0	32.0	38.0	25.0	40.0
70-71	33.41953398076855	35.0	32.0	38.0	26.0	40.0
72-73	33.21694183347521	35.0	32.0	37.0	25.5	40.0
74-75	32.94620253381106	35.0	32.0	37.0	25.0	39.5
76-77	32.71474832279061	35.0	32.0	36.0	25.0	39.0
78-79	32.50417488962306	35.0	32.0	36.0	25.0	39.0
80-81	32.280243486186805	35.0	31.0	35.5	24.5	38.0
82-83	32.04882229244619	35.0	31.0	35.0	24.0	37.0
84-85	31.860332971568873	35.0	31.0	35.0	24.0	37.0
86-87	31.690738601682273	34.5	31.0	35.0	24.0	36.0
88-89	31.689794786485976	35.0	31.0	35.0	24.0	36.0
90-91	31.615035966595407	35.0	31.0	35.0	24.0	36.0
92-93	31.477082757384238	35.0	31.0	35.0	24.0	36.0
94-95	31.450883118145065	35.0	31.0	35.0	24.0	35.0
96-97	31.36574352861315	35.0	31.0	35.0	23.0	35.0
98-99	31.29188661079162	35.0	31.0	35.0	23.0	35.0
100	30.800737807898965	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6052252629762904
1101	2	0.7539197687862433
1101	3	0.8268308412286522
1101	4	0.8669934086354729
1101	5	0.7541309433689563
1101	6	0.4707984332387909
1101	7	0.5557304898539357
1101	8	0.6015593693914454
1101	9	0.7552058954091336
1101	10-11	0.7755905297601586
1101	12-13	0.6667483799400031
1101	14-15	0.8964775492390586
1101	16-17	1.0030867883412355
1101	18-19	1.0996218278275052
1101	20-21	0.8342889237508331
1101	22-23	1.1455924853815844
1101	24-25	0.9825462596182106
1101	26-27	0.8319742039930631
1101	28-29	0.9454765070394302
1101	30-31	0.9497518687278017
1101	32-33	0.8130430212486175
1101	34-35	0.7659365058419922
1101	36-37	0.7563385101102256
1101	38-39	0.7656864193782624
1101	40-41	0.6938636082296838
1101	42-43	0.6692105844469296
1101	44-45	0.8416965466308071
1101	46-47	0.8517502976962703
1101	48-49	0.7449605421928283
1101	50-51	0.7995058268122648
1101	52-53	0.8087877520944105
1101	54-55	0.8651057636661861
1101	56-57	0.7907135392643738
1101	58-59	0.635031429387098
1101	60-61	0.6340152338148712
1101	62-63	0.6109892917769315
1101	64-65	0.6308388279012576
1101	66-67	0.675838414871599
1101	68-69	0.6422973485821899
1101	70-71	0.5446184870400401
1101	72-73	0.5099789570171112
1101	74-75	0.6012329707419539
1101	76-77	0.5761722695750322
1101	78-79	0.5204641242724932
1101	80-81	0.5528931272661808
1101	82-83	0.5728910824125109
1101	84-85	0.5885199506438497
1101	86-87	0.5489918444105903
1101	88-89	0.5744151787706002
1101	90-91	0.6676471180019199
1101	92-93	0.6628497249424576
1101	94-95	0.5887477564933974
1101	96-97	0.5664041374597062
1101	98-99	0.6111093038192159
1101	100	0.7251236684561739
1102	1	0.6271122183823685
1102	2	0.8277184852239152
1102	3	0.7502144734909386
1102	4	0.8700321762791319
1102	5	0.7271276548434429
1102	6	0.5546987492846895
1102	7	0.6052763950741067
1102	8	0.6223768848434545
1102	9	0.7795030517741992
1102	10-11	0.6996905517874197
1102	12-13	0.7234728785855324
1102	14-15	0.8840640955213104
1102	16-17	1.0163140645821116
1102	18-19	1.0786653939348838
1102	20-21	0.7622578489589955
1102	22-23	1.124285462314937
1102	24-25	1.0244760732499927
1102	26-27	0.773514361594593
1102	28-29	1.0330861587373619
1102	30-31	1.0494949340678446
1102	32-33	0.9306595717577153
1102	34-35	0.7717628066490008
1102	36-37	0.7515307882793678
1102	38-39	0.8377983795317618
1102	40-41	0.7209116403528313
1102	42-43	0.8013166775405196
1102	44-45	0.9013881153478991
1102	46-47	0.8209275372352138
1102	48-49	0.7651925938810891
1102	50-51	0.8583053596037971
1102	52-53	0.7607408159901823
1102	54-55	0.7230698732538272
1102	56-57	0.654910696852923
1102	58-59	0.6598649932823832
1102	60-61	0.6793440373804458
1102	62-63	0.6489577383762537
1102	64-65	0.5650115033119789
1102	66-67	0.6086929237394827
1102	68-69	0.7127549440973411
1102	70-71	0.5799630462584204
1102	72-73	0.5784013868302509
1102	74-75	0.603160343984321
1102	76-77	0.5300763090195204
1102	78-79	0.5296124672757472
1102	80-81	0.6398611806187304
1102	82-83	0.5881609074005851
1102	84-85	0.5283376086472344
1102	86-87	0.5567377135264984
1102	88-89	0.5527476990314284
1102	90-91	0.5688958651694698
1102	92-93	0.6548813331886087
1102	94-95	0.6206280502832868
1102	96-97	0.6315238850617533
1102	98-99	0.6776410881099828
1102	100	0.7143799079848456
1103	1	0.537473586768094
1103	2	0.7143156429815178
1103	3	0.6388001449180294
1103	4	0.6181849943995346
1103	5	0.6265866580459587
1103	6	0.43244671668282564
1103	7	0.4574016931874425
1103	8	0.4574953835067106
1103	9	0.5752099358012615
1103	10-11	0.6307953283645915
1103	12-13	0.5466915954435407
1103	14-15	0.7279389371713236
1103	16-17	0.8704887137186432
1103	18-19	0.9545302654943413
1103	20-21	0.6782961644480068
1103	22-23	0.8493382433559233
1103	24-25	0.7729229805578157
1103	26-27	0.6119603223400532
1103	28-29	0.7742627847558836
1103	30-31	0.8730480846436635
1103	32-33	0.7130652167314366
1103	34-35	0.6546299500039225
1103	36-37	0.6094360013468005
1103	38-39	0.6253883653507231
1103	40-41	0.6178127728526448
1103	42-43	0.7077625657482542
1103	44-45	0.6849498990121106
1103	46-47	0.5953157486092309
1103	48-49	0.5567844731911364
1103	50-51	0.6302387368169562
1103	52-53	0.6807788497543896
1103	54-55	0.6373745645808455
1103	56-57	0.5177247539497216
1103	58-59	0.5265438823726143
1103	60-61	0.5307848294008224
1103	62-63	0.5018801729712905
1103	64-65	0.576873550845896
1103	66-67	0.5813029258950095
1103	68-69	0.5428583566080789
1103	70-71	0.5303584157870773
1103	72-73	0.40448857232208724
1103	74-75	0.3857313282621888
1103	76-77	0.46486164245927597
1103	78-79	0.47105439636160185
1103	80-81	0.47706207068959827
1103	82-83	0.3971969190383078
1103	84-85	0.4629842091076455
1103	86-87	0.5738493812791639
1103	88-89	0.6067524248825578
1103	90-91	0.6220377172252007
1103	92-93	0.7152677760892168
1103	94-95	0.6091619149371326
1103	96-97	0.5797818645664563
1103	98-99	0.6028749341210933
1103	100	0.6215496622445258
1104	1	0.5068827989370881
1104	2	0.666410750896862
1104	3	0.6375654203577703
1104	4	0.7204360351990005
1104	5	0.6447908949056469
1104	6	0.3329736274831774
1104	7	0.3167873172220368
1104	8	0.30334115665425543
1104	9	0.508965381995587
1104	10-11	0.5050929626414415
1104	12-13	0.4199916996608337
1104	14-15	0.61619567215741
1104	16-17	0.6900666866148271
1104	18-19	0.6202248389281451
1104	20-21	0.5531164707985141
1104	22-23	0.7690389383329048
1104	24-25	0.6164274490199162
1104	26-27	0.5464824912156558
1104	28-29	0.73206264262231
1104	30-31	0.7359492389217408
1104	32-33	0.5103212017443042
1104	34-35	0.40336966578769307
1104	36-37	0.4326691948736183
1104	38-39	0.4926941105137459
1104	40-41	0.4069148367909534
1104	42-43	0.41743312467372107
1104	44-45	0.5558109934370421
1104	46-47	0.5168229942636273
1104	48-49	0.3857920273703499
1104	50-51	0.4428892278057788
1104	52-53	0.47959161769249903
1104	54-55	0.40167753143494167
1104	56-57	0.41556451748505197
1104	58-59	0.4558474819091529
1104	60-61	0.5333040578775226
1104	62-63	0.40397003049334756
1104	64-65	0.394118928769025
1104	66-67	0.4816259624276924
1104	68-69	0.4903414261554957
1104	70-71	0.3779549751201614
1104	72-73	0.3878319396907344
1104	74-75	0.3336536129724976
1104	76-77	0.402829038566594
1104	78-79	0.433613419737533
1104	80-81	0.39224916938122334
1104	82-83	0.41502659360013183
1104	84-85	0.37707830589238966
1104	86-87	0.3507551009651557
1104	88-89	0.32101206426742124
1104	90-91	0.29994464738255644
1104	92-93	0.29958136521920764
1104	94-95	0.26899864847518984
1104	96-97	0.3694678812214818
1104	98-99	0.3794795657496124
1104	100	0.4636040305918421
1105	1	0.34686209953935077
1105	2	0.47380241190169414
1105	3	0.4453390366449028
1105	4	0.5590855589954415
1105	5	0.428066384085092
1105	6	0.06934491052462022
1105	7	0.19795775474270272
1105	8	0.2101039272456049
1105	9	0.2601491398799709
1105	10-11	0.44517686786283406
1105	12-13	0.3126542460899273
1105	14-15	0.41842043651457317
1105	16-17	0.5394275949249518
1105	18-19	0.5141192697844232
1105	20-21	0.3753096854101088
1105	22-23	0.6729149803085548
1105	24-25	0.48328743412007924
1105	26-27	0.3194300796937313
1105	28-29	0.489534241316278
1105	30-31	0.5188976891363026
1105	32-33	0.37350992358528856
1105	34-35	0.3615773070643016
1105	36-37	0.2770462535432543
1105	38-39	0.3739512090403494
1105	40-41	0.2684488473590747
1105	42-43	0.2816608260237672
1105	44-45	0.3506471095441839
1105	46-47	0.151029037007973
1105	48-49	0.31510404930320135
1105	50-51	0.40367361624203113
1105	52-53	0.3304073764381812
1105	54-55	0.2978667953502665
1105	56-57	0.2647341823227265
1105	58-59	0.3094415987078989
1105	60-61	0.24301516348986496
1105	62-63	0.18282408046653842
1105	64-65	0.2245296536444812
1105	66-67	0.3174220086743631
1105	68-69	0.27346831563457386
1105	70-71	0.27412955185579335
1105	72-73	0.17368371082505263
1105	74-75	0.09204917483022257
1105	76-77	0.2044025605279174
1105	78-79	0.2351983502924213
1105	80-81	0.27631594974985063
1105	82-83	0.3109505578715215
1105	84-85	0.24527130207926007
1105	86-87	0.26006322846937024
1105	88-89	0.27256966957925854
1105	90-91	0.25588007341377406
1105	92-93	0.2841141035630059
1105	94-95	0.1387392037657733
1105	96-97	0.19950156869380464
1105	98-99	0.2438834024928589
1105	100	0.2185240031846334
1106	1	0.3743999381588452
1106	2	0.47028431860002584
1106	3	0.467091970397739
1106	4	0.573336279835388
1106	5	0.47438045996390343
1106	6	0.2428149332234142
1106	7	0.28708665582330894
1106	8	0.3111737939449881
1106	9	0.5037310351850905
1106	10-11	0.4467172524276535
1106	12-13	0.371372246493074
1106	14-15	0.49544711416546505
1106	16-17	0.6221559193336006
1106	18-19	0.5414965078938536
1106	20-21	0.4593191987445522
1106	22-23	0.6559948511270193
1106	24-25	0.48514595230101776
1106	26-27	0.4159965591026804
1106	28-29	0.540371971262033
1106	30-31	0.4881605481321216
1106	32-33	0.3959027397250878
1106	34-35	0.4258177301907722
1106	36-37	0.3481676307200132
1106	38-39	0.4512921860523491
1106	40-41	0.3900213520259186
1106	42-43	0.3579672114825172
1106	44-45	0.4011132454038915
1106	46-47	0.4087926365627581
1106	48-49	0.3745950355174159
1106	50-51	0.3773353516915776
1106	52-53	0.34086886737181743
1106	54-55	0.3918384700652169
1106	56-57	0.32241353781732585
1106	58-59	0.39593614749276185
1106	60-61	0.27956996485354324
1106	62-63	0.2796451544084917
1106	64-65	0.38209244778661144
1106	66-67	0.31327411159892193
1106	68-69	0.24718975591221692
1106	70-71	0.23863694868147434
1106	72-73	0.19284534548847176
1106	74-75	0.35556087040352224
1106	76-77	0.3326708795028779
1106	78-79	0.32671182934205234
1106	80-81	0.3139480672226611
1106	82-83	0.24077722763480125
1106	84-85	0.304267559846366
1106	86-87	0.3780018453330847
1106	88-89	0.3402398885919027
1106	90-91	0.3025502968084268
1106	92-93	0.27173526602148357
1106	94-95	0.3382097479232442
1106	96-97	0.3676922604372308
1106	98-99	0.34847004789219227
1106	100	0.27614212014913164
1107	1	0.2845589048751904
1107	2	0.42816107423386285
1107	3	0.3875907736312669
1107	4	0.4895838524316929
1107	5	0.3788118829869802
1107	6	0.1665194899682092
1107	7	0.18476650950167084
1107	8	0.18779146092778376
1107	9	0.21483138292478543
1107	10-11	0.2541976073931096
1107	12-13	0.21786549492372842
1107	14-15	0.3666201711134889
1107	16-17	0.526843179415792
1107	18-19	0.6123954375781722
1107	20-21	0.37211432587276505
1107	22-23	0.6188823324981172
1107	24-25	0.3098940668293082
1107	26-27	0.28095410776366236
1107	28-29	0.3862990136682072
1107	30-31	0.36615449655825927
1107	32-33	0.29658162244197683
1107	34-35	0.1971194959858309
1107	36-37	0.13440696143400999
1107	38-39	0.17521678976458332
1107	40-41	0.07173622805897395
1107	42-43	0.18951183054949183
1107	44-45	0.09929698292816624
1107	46-47	0.1814061035726482
1107	48-49	0.09536006283981635
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2216	94-95	-0.03757662988699906
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2216	98-99	-0.19907841587718877
2216	100	-0.10168720451996549
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	12088.0
1	1976.0
2	342.0
3	280.0
4	286.0
5	161.0
6	137.0
7	160.0
8	323.0
9	638.0
10	1076.0
11	2239.0
12	3702.0
13	5030.0
14	6482.0
15	8188.0
16	9682.0
17	11568.0
18	13323.0
19	14947.0
20	17100.0
21	19922.0
22	22710.0
23	26647.0
24	30209.0
25	33960.0
26	38440.0
27	44154.0
28	52386.0
29	62954.0
30	74775.0
31	88698.0
32	109732.0
33	139659.0
34	194202.0
35	261404.0
36	382808.0
37	577555.0
38	483977.0
39	168610.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.862086935726786	21.521606757904646	12.837848578867447	23.778457727501127
2	15.21538743893478	20.022174915125614	41.08408252147894	23.678355124460666
3	18.37344861706963	27.067209803976848	29.113120217951728	25.446221361001797
4	12.00072327682905	17.164555447728638	40.628872702246795	30.20584857319551
5	13.048086112316737	38.57280855762282	35.204488573238116	13.174616756822328
6	29.190493479773682	38.82116756197722	18.616339486358548	13.37199947189055
7	26.55253130234466	31.852033470322823	24.08974662733162	17.505688600000894
8	25.784156412423805	35.13282084471898	20.383836869330533	18.699185873526687
9	25.939695108340306	15.282416708197777	21.93987946175681	36.83800872170511
10-11	25.440837064557037	26.057548876264292	27.792982828488	20.70863123069067
12-13	25.67422399271866	23.876402272047915	27.7148086601446	22.734565075088824
14-15	23.145601619708657	25.082437866537422	25.196792297165356	26.575168216588562
16-17	22.06895104623286	27.53855576334039	26.61341351347493	23.779079676951813
18-19	21.55986552634308	28.20482965231161	28.115120925334512	22.1201838960108
20-21	23.298330763535297	27.342782966581737	26.908700651063278	22.45018561881969
22-23	23.695324921465392	27.0032514152734	26.675118420835464	22.626305242425744
24-25	23.111666893401534	27.15469641290535	27.424559634165156	22.30907705952796
26-27	22.929586822674604	27.42883170982207	26.459315984508798	23.18226548299453
28-29	22.12371469428829	27.891056562737088	27.02015425388128	22.965074489093343
30-31	23.01872723711474	27.369287578762442	26.901489274585032	22.710495909537787
32-33	23.41137848430183	27.698748583407422	25.95089260379604	22.938980328494708
34-35	23.353828743221467	27.491052248676112	26.922403518216264	22.23271548988616
36-37	23.059422115501555	27.2095555937438	26.607006992749564	23.124015298005077
38-39	23.174896888943472	27.177922752751737	27.23423629361268	22.412944064692113
40-41	22.77710664834406	26.322441771279433	27.849503481759875	23.050948098616637
42-43	22.72294283684601	26.629690851452857	27.167075757132707	23.480290554568427
44-45	22.894336254231128	27.551505057398145	26.92116018686544	22.632998501505288
46-47	22.810692442199006	27.257350215412508	26.504124618437313	23.42783272395117
48-49	23.598557279323085	26.72066866075754	26.774909124866053	22.905864935053323
50-51	23.154010516200593	26.69675685409739	26.693454524252086	23.45577810544993
52-53	22.538421584881778	27.37606717069915	26.573221152853932	23.512290091565138
54-55	22.640570564139875	27.228183515526165	26.5343457355181	23.59690018481586
56-57	23.634090057788796	26.6038101874894	26.329880085068297	23.432219669653502
58-59	22.64299569369547	27.009170203083244	27.208645080909612	23.139189022311673
60-61	22.62824865286546	27.56423353496496	26.546048852502096	23.261468959667482
62-63	23.071028457505488	26.865540225532424	26.145155823325076	23.918275493637005
64-65	22.15186139725832	27.169907375159415	26.606350017155933	24.071881210426334
66-67	23.050062852237822	26.62477205422164	26.53640571192607	23.78875938161447
68-69	22.411479108594847	26.839777169354456	26.74674757672361	24.001996145327087
70-71	22.43813580282162	26.44084920364369	26.95845242587596	24.162562567658725
72-73	23.091924142398295	26.58765664490176	27.256427906279146	23.063991306420803
74-75	23.19271870785513	26.927826050911737	26.85004858081917	23.02940666041397
76-77	22.784368433778784	26.918441534856413	26.492356226303993	23.804833805060813
78-79	22.639177300801673	26.822637611834104	26.68377555473861	23.854409532625617
80-81	22.252019438340916	27.056225758828635	27.371386784184608	23.320368018645837
82-83	23.13823337904618	26.16381159152596	28.181196231940586	22.51675879748727
84-85	23.440068138552977	26.73528337285837	26.800659645038316	23.023988843550335
86-87	22.137492029227545	27.380483270349277	27.24384145685017	23.238183243573005
88-89	23.092257903357165	26.07557759360964	27.117093615522524	23.715070887510663
90-91	22.923904849840746	26.054743756639986	27.388552879976725	23.632798513542543
92-93	23.069449400543164	26.4317974606824	27.17527857406885	23.323474564705585
94-95	21.88112999056911	26.38562436559463	27.848968795039752	23.884276848796507
96-97	22.289533508226597	26.299423101304846	27.364315166954338	24.046728223514215
98-99	21.724106857625273	27.04561009278168	27.257851020394398	23.97243202919865
100	22.532009950964657	26.357915112404946	26.456117242066345	24.65395769456405
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10166.0
1	5961.0
2	1626.0
3	1174.0
4	613.5
5	320.0
6	243.5
7	233.0
8	234.0
9	238.5
10	235.0
11	223.0
12	219.5
13	222.0
14	238.0
15	331.0
16	413.0
17	423.5
18	506.5
19	653.0
20	808.5
21	1055.5
22	1465.5
23	2049.5
24	2868.5
25	3888.5
26	5463.0
27	7429.5
28	10425.0
29	16547.5
30	24949.5
31	33002.5
32	43341.0
33	53114.0
34	60886.0
35	69630.5
36	77582.0
37	92031.0
38	98119.5
39	90090.5
40	82807.0
41	76466.0
42	75365.0
43	80254.0
44	87880.0
45	104913.0
46	120850.5
47	130831.0
48	138540.0
49	136364.0
50	129881.0
51	130447.5
52	129715.5
53	123686.0
54	116673.0
55	101904.5
56	89946.0
57	75589.5
58	60311.0
59	55241.0
60	45495.0
61	32249.5
62	24074.5
63	19957.5
64	14661.0
65	7645.0
66	4434.0
67	3032.5
68	2206.0
69	1685.0
70	1291.5
71	990.0
72	754.5
73	616.0
74	544.5
75	438.5
76	307.5
77	208.0
78	141.0
79	96.5
80	57.5
81	42.0
82	33.0
83	23.5
84	17.0
85	11.0
86	8.0
87	9.0
88	9.0
89	9.0
90	8.5
91	7.5
92	4.0
93	4.5
94	6.5
95	5.0
96	3.5
97	3.0
98	3.5
99	4.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.993392438255976
2	0.4488297170573084
3	0.4850974026862722
4	0.4697349584906073
5	0.43606759749164475
6	0.4866712833833782
7	0.5030601715119382
8	0.5067211548725978
9	0.5218783102816836
10-11	0.5337679525043864
12-13	0.5322111792061619
14-15	0.5310991982788589
16-17	0.5334600193245178
18-19	0.5312018426721483
20-21	0.5318519238296486
22-23	0.4940616797002236
24-25	0.5302780431325427
26-27	0.5087227205417435
28-29	0.5145221287626014
30-31	0.5185423674997741
32-33	0.5203044295845777
34-35	0.5322796088016883
36-37	0.5494383298799197
38-39	0.5396871125174153
40-41	0.5447166877886018
42-43	0.5425440481306403
44-45	0.5518333657233419
46-47	0.5501568406329465
48-49	0.5230929356022762
50-51	0.536265632741098
52-53	0.5300556469470821
54-55	0.5393791793375468
56-57	0.5269421003506333
58-59	0.5330323343524781
60-61	0.5279343294857652
62-63	0.5213137661185913
64-65	0.5321940718072803
66-67	0.5302096135370163
68-69	0.5512003919647231
70-71	0.5357695181735319
72-73	0.5385067019945858
74-75	0.5371894322807037
76-77	0.523931198147474
78-79	0.5553232750951855
80-81	0.5292344918007659
82-83	0.5280369738790548
84-85	0.536967036095243
86-87	0.8425051801203812
88-89	1.1071224260207644
90-91	1.0976449270403652
92-93	1.096704020101878
94-95	1.1020586359518147
96-97	1.0947537766293771
98-99	1.0912296524597702
100	1.0858408218120703
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2922712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.448446872357394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18820534716113	26.590137910742932
2	12.348601333385147	8.5078027396208
3	4.298222296623459	4.442012472924458
4	1.9888316672379436	2.7404864810763314
5	1.0611651691571506	1.8277745976253128
6	0.6079540404372958	1.2565843477703524
7	0.42300420684593953	1.020028656242122
8	0.2715989871479849	0.7484930623482751
9	0.20598466340187033	0.6386266560347775
>10	1.136882345387525	7.636225575460573
>50	0.18297839745082115	4.486774923682891
>100	0.22580179364326902	16.863288414692395
>500	0.04107677189159083	9.718693261246813
>1k	0.018897304260053892	11.36612510855669
>5k	7.956759688443743E-4	2.156945791975251
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	9154	0.31320225872408913	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	9037	0.30919912738579786	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	8839	0.3024245974286895	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7944	0.27180235343064935	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	7898	0.2702284727335434	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	7142	0.2443620856245843	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6979	0.23878507358918705	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	5961	0.20395440946627655	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	4509	0.15427452311414877	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	4501	0.1540008047320434	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4277	0.14633669003309255	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	4106	0.1404859596155899	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	4041	0.13826199776098363	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	3923	0.1342246516249292	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	3779	0.1292977207470322	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	3734	0.1277580548476894	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	3686	0.12611574455505709	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	3581	0.1225231907899239	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	3565	0.1219757540257131	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	3452	0.1181094818784745	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	3446	0.11790419309189548	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	3429	0.11732254152992154	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	3291	0.11260089943860359	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	3287	0.1124640402475509	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	3149	0.10774239815623297	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	3118	0.1066817394255746	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	3105	0.10623694705465335	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	3083	0.10548422150386354	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	3048	0.10428670358215246	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.421479776317338E-5
7	0.0	0.0	0.0	0.0	3.421479776317338E-5
8	0.0	0.0	0.0	0.0	3.421479776317338E-5
9	0.0	0.0	0.0	0.0	3.421479776317338E-5
10-11	0.0	0.0	0.0	0.0	3.421479776317338E-5
12-13	0.0	0.0	0.0	0.0	1.0264439328952015E-4
14-15	0.0	0.0	0.0	0.0	1.0264439328952015E-4
16-17	0.0	0.0	0.0	0.0	1.0264439328952015E-4
18-19	0.0	0.0	0.0	1.710739888158669E-5	1.3685919105269352E-4
20-21	0.0	0.0	0.0	3.421479776317338E-5	1.3685919105269352E-4
22-23	0.0	0.0	0.0	8.553699440793345E-5	2.3950358434221368E-4
24-25	0.0	0.0	0.0	1.1975179217110684E-4	2.3950358434221368E-4
26-27	0.0	0.0	0.0	2.5661098322380033E-4	3.421479776317338E-4
28-29	0.0	0.0	0.0	9.237995396056813E-4	3.763627753949072E-4
30-31	0.0	0.0	0.0	0.00460189029914682	3.763627753949072E-4
32-33	0.0	0.0	0.0	0.013617489509743006	3.763627753949072E-4
34-35	0.0	0.0	0.0	0.027594234395999333	3.763627753949072E-4
36-37	0.0	0.0	0.0	0.054452850640090436	3.763627753949072E-4
38-39	0.0	0.0	0.0	0.09662258888320163	3.934701742764939E-4
40-41	0.0	0.0	0.0	0.1452589239035526	4.105775731580806E-4
42-43	0.0	0.0	0.0	0.19966045234699825	4.105775731580806E-4
44-45	0.0	0.0	0.0	0.26779922209235807	4.276849720396673E-4
46-47	0.0	0.0	0.0	0.35537199696720034	4.4479237092125395E-4
48-49	0.0	0.0	0.0	0.45468044747481107	4.4479237092125395E-4
50-51	0.0	0.0	0.0	0.5623715234343993	4.4479237092125395E-4
52-53	0.0	0.0	0.0	0.6709692915347116	4.7900716868442736E-4
54-55	0.0	0.0	0.0	0.7906697615091737	4.7900716868442736E-4
56-57	0.0	0.0	0.0	0.9407016497006889	4.7900716868442736E-4
58-59	0.0	0.0	0.0	1.1255813094139961	4.7900716868442736E-4
60-61	0.0	0.0	0.0	1.3202806160853344	4.7900716868442736E-4
62-63	0.0	0.0	0.0	1.5224558560679258	5.132219664476007E-4
64-65	0.0	0.0	0.0	1.7364694160765755	5.132219664476007E-4
66-67	0.0	0.0	0.0	1.9836200077188584	5.132219664476007E-4
68-69	0.0	0.0	0.0	2.2667474592091184	5.132219664476007E-4
70-71	0.0	0.0	0.0	2.586211025923868	5.132219664476007E-4
72-73	0.0	0.0	0.0	2.9257586789256003	5.645441630923607E-4
74-75	0.0	0.0	0.0	3.2556235441603554	6.500811575002942E-4
76-77	0.0	0.0	0.0	3.637734405579476	6.500811575002942E-4
78-79	0.0	0.0	0.0	4.052742795047887	6.500811575002942E-4
80-81	0.0	3.421479776317338E-5	0.0	4.506602087376382	6.500811575002942E-4
82-83	0.0	3.421479776317338E-5	0.0	4.975190850141923	6.500811575002942E-4
84-85	0.0	3.421479776317338E-5	0.0	5.431992615078051	6.842959552634676E-4
86-87	0.0	3.421479776317338E-5	0.0	5.928620404610513	7.014033541450544E-4
88	0.0	3.421479776317338E-5	0.0	6.327718913119049	7.18510753026641E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	2770	0.0	36.631214	94
GTACTAG	2965	0.0	34.198425	1
CAACGCA	5810	0.0	32.75147	5
GTATCAA	6490	0.0	31.828909	1
TAGGCAT	3735	0.0	30.568037	5
ATCAACG	6300	0.0	30.132668	3
GCATCGT	3040	0.0	29.657385	8
TATTCTC	1685	0.0	28.720314	5
ACCGTGT	385	0.0	28.05255	8
AACGCAG	7385	0.0	27.33732	6
GGTATCA	4290	0.0	27.153887	1
ACGCAGA	7010	0.0	27.062075	7
TATCAAC	7695	0.0	26.869728	2
CTAGGCA	4425	0.0	26.546577	4
CTGTGCG	3865	0.0	26.366068	9
AGGGCTA	3825	0.0	26.28659	5
CGCAGAG	7110	0.0	26.219603	8
GAACCGT	345	0.0	25.856663	6
TAGTACT	4100	0.0	24.868961	4
GCTATTG	3780	0.0	24.721025	8
>>END_MODULE