FastQCFastQC Report
Mon 16 Jan 2017
ERR1146419_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146419_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966131
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122440.3087139582631032No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC108590.2737932761172034No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT93400.2354939864568266No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA89550.22578679322493383No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC80590.20319550715798343No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA70570.1779315912661483No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC68860.17362008466185308No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC56690.14293526865350642No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT49240.1241512194125711No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC47600.12001620723067392No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG46390.11696537507207905No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC45370.11439359920285035No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG42550.10728339532910032No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT42270.10657741763950813No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT41420.1044342710818175No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT40770.10279539430240706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74200.032.683381
GTACTAG38900.032.262631
CAACGCA71000.030.312285
GGTATCA48700.029.4518361
GGGCACC36750.029.29130294
TAGGCAT45000.029.238635
GCATCGT39100.028.819238
ATCAACG75700.028.3746813
CTAGGCA50950.027.3018514
TATCAAC91200.026.387752
TATTCTC21400.026.1303165
CGTTAAC7950.026.1127911
AACGCAG91250.025.4675486
TCTAACG17050.025.362422
AGGGCTA41250.025.2895135
GTACATG161950.024.2678781
CTATTGA43650.024.2023289
CTGTGCG49100.023.9065769
CTAACGC17550.023.8354633
TACATGG162100.023.7770542
Produced by FastQC (version 0.11.5)