##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146419_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3966131
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.428967676559346	31.0	30.0	34.0	27.0	34.0
2	30.543194866735366	31.0	31.0	34.0	26.0	34.0
3	30.737319316986756	31.0	31.0	34.0	27.0	34.0
4	34.226954429896544	37.0	35.0	37.0	30.0	37.0
5	34.434480353775506	37.0	35.0	37.0	30.0	37.0
6	34.65105867657927	37.0	35.0	37.0	31.0	37.0
7	34.554203580264996	37.0	35.0	37.0	30.0	37.0
8	34.49459536258384	37.0	35.0	37.0	30.0	37.0
9	35.918733395341704	39.0	35.0	39.0	30.0	39.0
10-11	35.83792756719332	39.0	35.0	39.0	30.0	39.0
12-13	35.83158977855245	39.0	35.0	39.0	30.0	39.0
14-15	36.8692313743545	40.0	36.0	41.0	30.0	41.0
16-17	36.686849476227586	40.0	36.0	41.0	29.0	41.0
18-19	36.62745847275342	40.0	36.0	41.0	28.5	41.0
20-21	36.959619462897216	40.0	36.0	41.0	30.0	41.0
22-23	36.67458828263615	40.0	36.0	41.0	29.0	41.0
24-25	36.78087990537882	40.0	36.0	41.0	30.0	41.0
26-27	36.85247095973381	40.0	36.0	41.0	30.0	41.0
28-29	36.683798265866656	40.0	36.0	41.0	29.5	41.0
30-31	36.632642240006696	40.0	36.0	41.0	29.5	41.0
32-33	36.739234407537225	40.0	36.0	41.0	30.0	41.0
34-35	36.790107790186454	40.0	36.0	41.0	30.0	41.0
36-37	36.716273995992566	40.0	35.0	41.0	30.0	41.0
38-39	36.55197912020556	39.0	35.0	41.0	29.0	41.0
40-41	36.51469139572042	39.0	35.0	41.0	29.0	41.0
42-43	36.35309247223554	39.0	35.0	41.0	29.0	41.0
44-45	36.17279951166515	39.0	35.0	41.0	28.0	41.0
46-47	36.043029466248086	39.0	35.0	41.0	28.0	41.0
48-49	35.91150922649807	39.0	34.5	40.0	28.0	41.0
50-51	35.703196011427764	38.0	34.0	40.0	27.5	41.0
52-53	35.512214674704396	38.0	34.0	40.0	27.0	41.0
54-55	35.31802719073072	38.0	34.0	40.0	27.0	41.0
56-57	35.16662346755566	37.0	33.5	40.0	27.0	41.0
58-59	34.91045958895458	37.0	33.0	40.0	26.5	41.0
60-61	34.651340689452766	36.5	33.0	40.0	26.0	41.0
62-63	34.37520533235034	36.0	33.0	39.0	26.0	41.0
64-65	34.08316631498052	35.0	33.0	39.0	26.0	41.0
66-67	33.78643935361691	35.0	32.0	39.0	26.0	40.5
68-69	33.49426342700228	35.0	32.0	38.0	26.0	40.0
70-71	33.409752728792874	35.0	32.0	37.5	26.0	40.0
72-73	33.19272648835855	35.0	32.0	37.0	26.0	40.0
74-75	32.92361699600946	35.0	32.0	36.5	25.0	39.0
76-77	32.68844410333395	35.0	32.0	36.0	25.0	39.0
78-79	32.478571433974324	35.0	32.0	36.0	25.0	39.0
80-81	32.25991703249338	35.0	31.5	35.0	24.5	37.0
82-83	32.04051530320103	35.0	31.0	35.0	24.0	37.0
84-85	31.863545354402063	35.0	31.0	35.0	24.0	36.5
86-87	31.703335189886566	34.5	31.0	35.0	24.0	36.0
88-89	31.717072128984142	35.0	32.0	35.0	24.0	36.0
90-91	31.648481605877365	35.0	32.0	35.0	24.0	36.0
92-93	31.521273755203747	35.0	31.0	35.0	24.0	35.5
94-95	31.499854770303855	35.0	31.0	35.0	24.0	35.0
96-97	31.417285510740818	35.0	31.0	35.0	23.5	35.0
98-99	31.342444563732265	35.0	31.0	35.0	23.0	35.0
100	30.856481039078133	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6318412565005893
1101	2	0.8751813600201608
1101	3	0.8538745007818953
1101	4	0.9569198805557093
1101	5	0.9186242632845207
1101	6	0.6402947125937501
1101	7	0.6972179410465458
1101	8	0.6758104018352782
1101	9	0.8731437759657794
1101	10-11	0.8876918509442078
1101	12-13	0.7561174745937151
1101	14-15	1.0504734414892027
1101	16-17	1.080273222917505
1101	18-19	1.2359894647275596
1101	20-21	0.9107015193260892
1101	22-23	1.1693034561438367
1101	24-25	1.0507118781634546
1101	26-27	1.0006101244471353
1101	28-29	1.187840695284649
1101	30-31	1.184227581693655
1101	32-33	1.017394748524282
1101	34-35	0.8827391760949652
1101	36-37	0.8468770289501393
1101	38-39	0.9875232656397941
1101	40-41	0.9149620387817308
1101	42-43	0.9204650916228729
1101	44-45	0.8991339443720818
1101	46-47	0.8811817137020128
1101	48-49	0.9492847827202766
1101	50-51	0.9789566314173612
1101	52-53	0.9963228578439356
1101	54-55	1.0717033513703598
1101	56-57	0.9435670183541376
1101	58-59	0.8269290470596715
1101	60-61	0.8472519809972638
1101	62-63	0.8417144370880862
1101	64-65	0.784752326041712
1101	66-67	0.7390425022414249
1101	68-69	0.7798447225237339
1101	70-71	0.7016022855506066
1101	72-73	0.6883108260613895
1101	74-75	0.6842372539579884
1101	76-77	0.7200335130814253
1101	78-79	0.7106370714956327
1101	80-81	0.7808655713332087
1101	82-83	0.7372720598599116
1101	84-85	0.715395892937174
1101	86-87	0.7420997842331367
1101	88-89	0.706449190497441
1101	90-91	0.7129187736365594
1101	92-93	0.7629152417993517
1101	94-95	0.7449175556777448
1101	96-97	0.7361636673454051
1101	98-99	0.755910142948423
1101	100	0.7607790978039333
1102	1	0.6579744937703396
1102	2	0.7980776809886052
1102	3	0.736811883265645
1102	4	0.7940386120239609
1102	5	0.6839018306911626
1102	6	0.4836165409157047
1102	7	0.6154226863794889
1102	8	0.5809511543559864
1102	9	0.7046614445401147
1102	10-11	0.6858012146741572
1102	12-13	0.6029359460517938
1102	14-15	0.832934770877003
1102	16-17	0.9933675433706028
1102	18-19	0.9984011604493759
1102	20-21	0.774071772214306
1102	22-23	1.0586615856455523
1102	24-25	0.9762914234789264
1102	26-27	0.8497639292849044
1102	28-29	0.9794447482944122
1102	30-31	1.0715816711412955
1102	32-33	0.892698595896718
1102	34-35	0.8251974238661788
1102	36-37	0.7125957931837732
1102	38-39	0.8087397831340866
1102	40-41	0.8176186165248467
1102	42-43	0.7897652458917861
1102	44-45	0.8360856955038898
1102	46-47	0.7942720648883252
1102	48-49	0.7558050838344741
1102	50-51	0.8629572400219843
1102	52-53	0.7848661664894081
1102	54-55	0.768118378003166
1102	56-57	0.6996386591984631
1102	58-59	0.6808528724577556
1102	60-61	0.6942188541806118
1102	62-63	0.6859624602283532
1102	64-65	0.6241663196148224
1102	66-67	0.784858026696007
1102	68-69	0.7480051574049043
1102	70-71	0.6350888789917519
1102	72-73	0.5746103362831789
1102	74-75	0.5639900509537341
1102	76-77	0.5443105673168276
1102	78-79	0.5594931691113558
1102	80-81	0.6145216854415452
1102	82-83	0.6048897457500146
1102	84-85	0.6145725717434516
1102	86-87	0.5772252109873399
1102	88-89	0.5616054915521396
1102	90-91	0.6033486784491515
1102	92-93	0.7090421912619895
1102	94-95	0.6518477880097961
1102	96-97	0.6290622851148093
1102	98-99	0.6561297252296221
1102	100	0.6519225302270186
1103	1	0.481987399413061
1103	2	0.6872629898338474
1103	3	0.5661249010643168
1103	4	0.5654115676421796
1103	5	0.5767134464494177
1103	6	0.36706447628843364
1103	7	0.4489583714309191
1103	8	0.48105197210263384
1103	9	0.5315048706342722
1103	10-11	0.63631192545202
1103	12-13	0.5559540991944942
1103	14-15	0.6256976954875455
1103	16-17	0.8376382608105715
1103	18-19	0.9059875593159461
1103	20-21	0.6344795202129134
1103	22-23	0.785530492347803
1103	24-25	0.7647887316268864
1103	26-27	0.6241805611418343
1103	28-29	0.8748576318677053
1103	30-31	0.871243248848593
1103	32-33	0.6612936675173771
1103	34-35	0.6208197728480513
1103	36-37	0.5399968427808872
1103	38-39	0.6201461973764282
1103	40-41	0.597905609020394
1103	42-43	0.6151553911482495
1103	44-45	0.7337435311186411
1103	46-47	0.7067677356735231
1103	48-49	0.6492716568335055
1103	50-51	0.7013479217186855
1103	52-53	0.7159025425763872
1103	54-55	0.7530535481253722
1103	56-57	0.5446429729355202
1103	58-59	0.5741657241893847
1103	60-61	0.6046978179902567
1103	62-63	0.5263180257867788
1103	64-65	0.5532642757398563
1103	66-67	0.5534191565442867
1103	68-69	0.5711268487345933
1103	70-71	0.542415288252073
1103	72-73	0.49231733162662294
1103	74-75	0.4947492358996044
1103	76-77	0.41792355851806207
1103	78-79	0.45129842831372713
1103	80-81	0.5374052016732023
1103	82-83	0.4292299661311816
1103	84-85	0.3709555247674743
1103	86-87	0.4806571781886113
1103	88-89	0.4548458710444585
1103	90-91	0.4799278600761099
1103	92-93	0.5131828689830407
1103	94-95	0.5022383793893468
1103	96-97	0.5759276908130637
1103	98-99	0.5439632629298146
1103	100	0.5731346448176495
1104	1	0.4696201021387445
1104	2	0.5378708392740315
1104	3	0.4833355086137878
1104	4	0.5315986023652002
1104	5	0.549540488768244
1104	6	0.2840940350130623
1104	7	0.26624520404375573
1104	8	0.31896187247620844
1104	9	0.43675789221382644
1104	10-11	0.5046010534599219
1104	12-13	0.36004242926058083
1104	14-15	0.5441596168294325
1104	16-17	0.6860126536125435
1104	18-19	0.5987039030651786
1104	20-21	0.507308723322268
1104	22-23	0.7472982782912467
1104	24-25	0.6102080908609793
1104	26-27	0.4097871414353449
1104	28-29	0.6426453675003216
1104	30-31	0.6952141437628612
1104	32-33	0.43453622985396123
1104	34-35	0.37825955892470375
1104	36-37	0.41851580136628996
1104	38-39	0.5319982096634419
1104	40-41	0.40114071917177085
1104	42-43	0.4030829273856611
1104	44-45	0.48422571152406135
1104	46-47	0.4918456849206194
1104	48-49	0.438970126142209
1104	50-51	0.5128920594876405
1104	52-53	0.47963794437093554
1104	54-55	0.4792599460735829
1104	56-57	0.3901303208079199
1104	58-59	0.368776420280426
1104	60-61	0.4090472478148328
1104	62-63	0.3208242031691242
1104	64-65	0.35815244148418657
1104	66-67	0.44513860051949905
1104	68-69	0.42192653824170634
1104	70-71	0.3048426237266284
1104	72-73	0.31629859833541474
1104	74-75	0.32883561744107226
1104	76-77	0.3708755733344802
1104	78-79	0.36782556510993913
1104	80-81	0.4082655941413975
1104	82-83	0.41872846063215974
1104	84-85	0.3680393345868964
1104	86-87	0.3280051065179812
1104	88-89	0.3345696610049451
1104	90-91	0.3700109500980773
1104	92-93	0.3052442135718074
1104	94-95	0.25871480878722863
1104	96-97	0.35834104215976836
1104	98-99	0.4233880757449775
1104	100	0.4518781985122118
1105	1	0.4265972528176185
1105	2	0.5813691103946468
1105	3	0.4860263349495284
1105	4	0.636150342565422
1105	5	0.508191765199463
1105	6	0.2799492421370928
1105	7	0.32670954691276677
1105	8	0.39660557825406073
1105	9	0.2938074855755701
1105	10-11	0.45164768821761925
1105	12-13	0.3608202361131134
1105	14-15	0.5251291613700104
1105	16-17	0.6151636639830684
1105	18-19	0.684239229156077
1105	20-21	0.44345175370354895
1105	22-23	0.7214166360363095
1105	24-25	0.6544727602371694
1105	26-27	0.4490443143331362
1105	28-29	0.6944467696970094
1105	30-31	0.7082843513527806
1105	32-33	0.5148835823306896
1105	34-35	0.4011338339732262
1105	36-37	0.3506561693854948
1105	38-39	0.47104310634619395
1105	40-41	0.3695312744407815
1105	42-43	0.3962264750838642
1105	44-45	0.4170144999333516
1105	46-47	0.4370684703993959
1105	48-49	0.47347636244759883
1105	50-51	0.5458751527100674
1105	52-53	0.5328229537339979
1105	54-55	0.4989025357167378
1105	56-57	0.5600922405138391
1105	58-59	0.5223045040871384
1105	60-61	0.4276765327445773
1105	62-63	0.42203681111104174
1105	64-65	0.37680786316614956
1105	66-67	0.4959091844710102
1105	68-69	0.390646759991256
1105	70-71	0.4334004557360629
1105	72-73	0.38766208134725133
1105	74-75	0.40407043040455903
1105	76-77	0.4238602263677578
1105	78-79	0.48994063046365
1105	80-81	0.45817893077597205
1105	82-83	0.4083633878189801
1105	84-85	0.3183099472697606
1105	86-87	0.40270699682711
1105	88-89	0.42505028955072177
1105	90-91	0.4206213176662814
1105	92-93	0.4931555192471926
1105	94-95	0.365596955233471
1105	96-97	0.39819001217713534
1105	98-99	0.4508837864559254
1105	100	0.47896655744697014
1106	1	0.443029905096914
1106	2	0.4747653027090699
1106	3	0.5146868747243722
1106	4	0.5769706011630049
1106	5	0.4578099710955357
1106	6	0.2680148933902089
1106	7	0.24657467014926482
1106	8	0.26750574069845356
1106	9	0.40315886008976065
1106	10-11	0.42856925245691
1106	12-13	0.30787456373501954
1106	14-15	0.425413570254328
1106	16-17	0.5408316338440784
1106	18-19	0.5808373480263711
1106	20-21	0.3746180574562672
1106	22-23	0.6860870528382748
1106	24-25	0.4745073414069054
1106	26-27	0.3905627758114534
1106	28-29	0.5525576180700966
1106	30-31	0.5435904146666033
1106	32-33	0.38259161510560347
1106	34-35	0.449567580364878
1106	36-37	0.2974631716064309
1106	38-39	0.3952893260600234
1106	40-41	0.32686463259125986
1106	42-43	0.41532118294561826
1106	44-45	0.4499351648699843
1106	46-47	0.43888361642008533
1106	48-49	0.4097072027909192
1106	50-51	0.4056177650852604
1106	52-53	0.292979087901756
1106	54-55	0.3741801897766308
1106	56-57	0.3745843098423407
1106	58-59	0.30968026325999887
1106	60-61	0.38452462009072264
1106	62-63	0.2679754511639274
1106	64-65	0.3653910695813707
1106	66-67	0.3329127085545096
1106	68-69	0.2996575707021236
1106	70-71	0.3303820509791393
1106	72-73	0.338476525082001
1106	74-75	0.34063354336900886
1106	76-77	0.33542598902825915
1106	78-79	0.35399653358183514
1106	80-81	0.4082256247719016
1106	82-83	0.4051940766796278
1106	84-85	0.3618207030739242
1106	86-87	0.43433345506115373
1106	88-89	0.4557726141068734
1106	90-91	0.4150838575361746
1106	92-93	0.4520333277226989
1106	94-95	0.4422566716284493
1106	96-97	0.504484721353311
1106	98-99	0.4862371936217329
1106	100	0.4576972350810493
1107	1	0.3220325188552522
1107	2	0.5165929917966672
1107	3	0.38621518062891624
1107	4	0.5094668154406179
1107	5	0.42517354780417094
1107	6	0.226140537101287
1107	7	0.24940788779402823
1107	8	0.26610893457208107
1107	9	0.34766005141205625
1107	10-11	0.33659730986478564
1107	12-13	0.2365880926998969
1107	14-15	0.3960120976053645
1107	16-17	0.5457800115242577
1107	18-19	0.6386667759980895
1107	20-21	0.37389545658499657
1107	22-23	0.696862410857193
1107	24-25	0.3272128884531753
1107	26-27	0.2730577999519781
1107	28-29	0.41877798661951005
1107	30-31	0.40460600753660714
1107	32-33	0.41583132925936184
1107	34-35	0.27680651465784933
1107	36-37	0.25039687929137955
1107	38-39	0.2788027816215859
1107	40-41	0.09310254702069898
1107	42-43	0.36334500721856244
1107	44-45	0.2654540883460399
1107	46-47	0.21471549261738687
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2216	5	-0.09169543959752957
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2216	90-91	-0.2834698382397498
2216	92-93	-0.22581648235669505
2216	94-95	-0.18100272466958245
2216	96-97	-0.24067255923421982
2216	98-99	-0.2819839404286597
2216	100	-0.3717753460875173
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16553.0
1	2550.0
2	388.0
3	399.0
4	401.0
5	236.0
6	206.0
7	237.0
8	469.0
9	923.0
10	1617.0
11	2980.0
12	4979.0
13	6658.0
14	8692.0
15	10566.0
16	12757.0
17	14922.0
18	17232.0
19	19432.0
20	22319.0
21	25581.0
22	29280.0
23	34197.0
24	38801.0
25	44225.0
26	50952.0
27	57785.0
28	69247.0
29	82772.0
30	98362.0
31	119143.0
32	147838.0
33	189512.0
34	261970.0
35	358262.0
36	534566.0
37	811670.0
38	665887.0
39	201414.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62868010809305	20.694047827939418	12.611314014073693	24.06595804989384
2	15.72356688640176	19.965179412897747	40.288442518297394	24.022811182403093
3	18.84280270927854	26.504270080602886	28.641780687079237	26.01114652303933
4	12.64771047808973	16.6945836493627	39.26255086236117	31.395155010186404
5	13.714639965376588	38.01472001555926	34.565952512337375	13.704687506726772
6	30.363375300654884	37.67433422663063	18.198941370492204	13.76334910222228
7	27.272213537225355	31.335246257039472	23.569558208893195	17.82298199684198
8	26.079682314824083	34.78181318256634	20.1954361899066	18.94306831270297
9	26.303662283403057	15.292125484924199	21.303436707792393	37.100775523880344
10-11	25.785827369250363	25.661782217169893	27.428566938183636	21.123823475396104
12-13	26.040437149189472	23.400946576104474	27.44573879158507	23.11287748312098
14-15	23.46991436331399	24.650641102821055	24.804044454527137	27.07540007933782
16-17	22.758250857396128	27.219879346661553	25.977705355862962	24.044164440079356
18-19	22.259014228011942	27.694781710365234	27.452208802561316	22.593995259061504
20-21	23.91455197619594	26.870173609073568	26.3772763860659	22.83799802866459
22-23	24.238538179799367	26.460251090553676	26.195371118923088	23.10583961072387
24-25	23.698068059904273	26.689986572043615	26.7211896193469	22.890755748705207
26-27	23.55795614792356	26.889341924882608	25.94592382279594	23.60677810439789
28-29	22.82451273178259	27.2139912433711	26.57059406368432	23.39090196116199
30-31	23.52841809983009	26.81146821357751	26.47188603765628	23.188227648936127
32-33	23.81357186596429	27.169115559773065	25.598536645700776	23.418775928561868
34-35	23.79766382508054	26.921917238577013	26.422391061582793	22.85802787475965
36-37	23.618940605312616	26.635932935274592	26.087332407273365	23.657794052139426
38-39	23.697456005994816	26.652186632230958	26.617025904355724	23.03333145741851
40-41	23.364081305256732	25.925950338467686	27.203449728764458	23.506518627511127
42-43	23.288384352760662	26.175124202798344	26.647218421371182	23.88927302306981
44-45	23.41704668346841	26.941014014510046	26.426871025764576	23.21506827625697
46-47	23.402117292830482	26.64170563869419	26.146812018004802	23.80936505047053
48-49	24.00097175929112	26.267028757256988	26.33935309573388	23.392646387718017
50-51	23.630200795638363	26.291644833889645	26.245292168820537	23.83286220165146
52-53	23.18986734064966	26.71609839485874	26.203412355743684	23.890621908747917
54-55	23.18384108172964	26.66847414626059	26.15815902753035	23.989525744479423
56-57	24.016997383272894	26.20620448340266	25.95601409359815	23.820784039726295
58-59	23.114725778467882	26.44734385616746	26.752580148449557	23.685350216915108
60-61	23.23848101958855	26.905711783964392	26.183676855024103	23.67213034142296
62-63	23.567881181810012	26.432365937883233	25.75907249262538	24.240680387681373
64-65	22.851037510582962	26.557178417346428	26.21707874738278	24.374705324687834
66-67	23.544102361904343	26.165062208174415	26.18656989853617	24.104265531385067
68-69	22.936646947261377	26.342107574982187	26.397859569624522	24.323385908131918
70-71	22.970171036963478	26.008201713221414	26.522928611127128	24.49869863868798
72-73	23.470654868593684	26.182208157462217	26.71533196434721	23.63180500959689
74-75	23.608982111672827	26.44352921761795	26.38931947700085	23.558169193708377
76-77	23.245283535354382	26.399947178738888	26.105298829187852	24.249470456718882
78-79	23.204186870165216	26.318706526480618	26.208365601673066	24.2687410016811
80-81	22.868731424159495	26.626426484505195	26.760477341197515	23.744364750137795
82-83	23.531115251449517	25.82414483642192	27.470222958532727	23.174516953595838
84-85	23.713736644953162	26.274729350138674	26.347989293401508	23.663544711506653
86-87	22.68179600115039	26.780236694425362	26.78405098331096	23.753916321113284
88-89	23.46135718344385	25.674714175145784	26.701247355102588	24.16268128630778
90-91	23.221615500087704	25.765816020591725	26.79912460686853	24.21344387245204
92-93	23.33017877474973	25.98941557213172	26.771010150506097	23.909395502612455
94-95	22.540251427848794	25.896107220538482	27.28188734826916	24.28175400334357
96-97	22.791529028808917	25.844826843353015	26.895616519449288	24.468027608388777
98-99	22.362487498747644	26.441012316553763	26.7662806903674	24.430219494331194
100	22.980400454267937	25.763084739324892	26.178579303787224	25.07793550261994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13646.0
1	7998.5
2	2227.5
3	1642.0
4	894.0
5	495.0
6	370.0
7	346.0
8	327.5
9	319.0
10	308.0
11	305.0
12	316.5
13	312.0
14	303.5
15	400.0
16	463.5
17	490.0
18	644.0
19	767.0
20	921.5
21	1187.0
22	1610.5
23	2288.5
24	3113.0
25	4316.0
26	6139.5
27	8203.5
28	11654.0
29	18231.0
30	26913.0
31	35887.5
32	47126.0
33	58310.5
34	68024.5
35	78063.0
36	88226.5
37	106205.0
38	115016.5
39	110377.0
40	106191.5
41	102009.0
42	103189.5
43	110750.5
44	122256.5
45	143100.0
46	162854.0
47	176970.0
48	187076.5
49	186060.0
50	179911.0
51	181763.5
52	183121.5
53	176784.0
54	168002.0
55	149436.5
56	133062.0
57	112321.5
58	91686.5
59	85162.0
60	71574.0
61	53426.0
62	41964.0
63	34338.5
64	25467.5
65	15356.5
66	10151.0
67	7768.0
68	6138.5
69	4687.5
70	3642.5
71	2863.5
72	2222.5
73	1871.5
74	1492.5
75	1124.5
76	857.0
77	573.5
78	390.5
79	266.0
80	175.5
81	123.5
82	81.5
83	58.5
84	44.5
85	31.5
86	30.0
87	24.0
88	17.0
89	14.5
90	12.0
91	12.5
92	11.5
93	7.5
94	6.5
95	8.5
96	6.0
97	2.5
98	3.0
99	3.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9962858009480775
2	0.4511197436494155
3	0.48535966159463717
4	0.47182001804781537
5	0.4376557405693357
6	0.48737674070775777
7	0.5040176433910024
8	0.5064381383267471
9	0.5211376023636133
10-11	0.5344377177657521
12-13	0.5312608181625872
14-15	0.5288781434602134
16-17	0.534336863810096
18-19	0.5327988409863417
20-21	0.5317398744519533
22-23	0.49485254017076086
24-25	0.5295715144053487
26-27	0.5083165432508406
28-29	0.5152250392132786
30-31	0.5180615567161044
32-33	0.5212888832970973
34-35	0.5318155149186954
36-37	0.5502087550814635
38-39	0.5392913143816984
40-41	0.5443340121644998
42-43	0.541421854194932
44-45	0.5521123734944711
46-47	0.5508895192821417
48-49	0.5221587486646306
50-51	0.5389131120479883
52-53	0.5301514246503708
54-55	0.5398586178822636
56-57	0.5278569971591962
58-59	0.5333787512313637
60-61	0.526772817135894
62-63	0.5212762765526404
64-65	0.5322819644636044
66-67	0.5302522786060269
68-69	0.5516081037161908
70-71	0.5351184819664303
72-73	0.5386231569254772
74-75	0.5362404822231036
76-77	0.5231798949656479
78-79	0.5544824414523877
80-81	0.5291428850938106
82-83	0.5278569971591962
84-85	0.5363917631565877
86-87	0.8459125530649391
88-89	1.11424710883226
90-91	1.1038213311663179
92-93	1.103468342321522
94-95	1.1086875345267213
96-97	1.1018294655421115
98-99	1.0973918914932463
100	1.092651755577413
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3966131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.581223672825885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5896901175511	26.539924477593562
2	12.789116845627222	9.101048541243351
3	4.9537633943234685	5.287828900670415
4	2.4264865603845203	3.4534944417659017
5	1.3101305585152183	2.3308024221567143
6	0.8560799710776751	1.827622375964464
7	0.5578132685931445	1.389337507423794
8	0.39423124983820246	1.1221784223448628
9	0.28746052412654316	0.9205377485448862
>10	1.4487471617410437	9.444336864254508
>50	0.1542948067401499	3.8573038675039544
>100	0.1810717492115987	14.059785391097387
>500	0.033273521284299126	8.27136761773147
>1k	0.017273913176757645	10.654608877291713
>5k	4.2476835680551586E-4	1.1578665258870264
>10k+	1.4158945226850527E-4	0.5819560185260404
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12244	0.3087139582631032	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	10859	0.2737932761172034	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	9340	0.2354939864568266	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	8955	0.22578679322493383	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	8059	0.20319550715798343	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	7057	0.1779315912661483	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6886	0.17362008466185308	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	5669	0.14293526865350642	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	4924	0.1241512194125711	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	4760	0.12001620723067392	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4639	0.11696537507207905	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	4537	0.11439359920285035	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4255	0.10728339532910032	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4227	0.10657741763950813	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	4142	0.1044342710818175	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4077	0.10279539430240706	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.5213488914007126E-5	2.5213488914007126E-5
18-19	0.0	0.0	0.0	2.5213488914007126E-5	2.5213488914007126E-5
20-21	0.0	0.0	0.0	2.5213488914007126E-5	2.5213488914007126E-5
22-23	0.0	0.0	0.0	6.303372228501781E-5	2.5213488914007126E-5
24-25	0.0	0.0	0.0	1.1346070011303207E-4	2.5213488914007126E-5
26-27	0.0	0.0	0.0	2.6474163359707485E-4	2.5213488914007126E-5
28-29	0.0	0.0	0.0	0.0012354609567863492	2.5213488914007126E-5
30-31	0.0	0.0	0.0	0.005231798949656479	2.5213488914007126E-5
32-33	0.0	0.0	0.0	0.01535501474863034	2.5213488914007126E-5
34-35	0.0	0.0	0.0	0.0314790409091379	2.5213488914007126E-5
36-37	0.0	0.0	0.0	0.06008374408207898	2.5213488914007126E-5
38-39	0.0	0.0	0.0	0.10585883320545891	2.5213488914007126E-5
40-41	0.0	0.0	0.0	0.15928621621424002	2.5213488914007126E-5
42-43	0.0	0.0	0.0	0.2164451955822942	2.5213488914007126E-5
44-45	0.0	0.0	0.0	0.2845216156501134	2.5213488914007126E-5
46-47	0.0	0.0	0.0	0.3728696808047944	2.5213488914007126E-5
48-49	0.0	0.0	0.0	0.47800992957620414	2.5213488914007126E-5
50-51	0.0	0.0	0.0	0.5932986076355018	2.5213488914007126E-5
52-53	0.0	0.0	0.0	0.7067340942596197	2.5213488914007126E-5
54-55	0.0	0.0	0.0	0.8337974716417587	2.5213488914007126E-5
56-57	0.0	0.0	0.0	0.9918986538770405	2.5213488914007126E-5
58-59	0.0	0.0	0.0	1.18122674213232	2.5213488914007126E-5
60-61	0.0	0.0	0.0	1.3812831699205095	3.782023337101069E-5
62-63	0.0	0.0	0.0	1.59197969003041	5.042697782801425E-5
64-65	0.0	0.0	0.0	1.815043426452631	5.042697782801425E-5
66-67	0.0	0.0	0.0	2.0726496426870415	5.042697782801425E-5
68-69	0.0	0.0	0.0	2.362894720320635	5.042697782801425E-5
70-71	0.0	0.0	0.0	2.6870897607769386	5.042697782801425E-5
72-73	0.0	0.0	0.0	3.0323884914542663	5.042697782801425E-5
74-75	0.0	0.0	0.0	3.378519267265756	5.042697782801425E-5
76-77	0.0	0.0	0.0	3.771509312223928	5.042697782801425E-5
78-79	0.0	0.0	0.0	4.200378151906732	5.042697782801425E-5
80-81	0.0	0.0	0.0	4.669954169441201	5.042697782801425E-5
82-83	0.0	0.0	0.0	5.153523673322944	5.042697782801425E-5
84-85	0.0	0.0	0.0	5.629352636108086	5.042697782801425E-5
86-87	0.0	0.0	0.0	6.139068527993654	5.042697782801425E-5
88	0.0	0.0	0.0	6.550867835681676	5.042697782801425E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7420	0.0	32.68338	1
GTACTAG	3890	0.0	32.26263	1
CAACGCA	7100	0.0	30.31228	5
GGTATCA	4870	0.0	29.451836	1
GGGCACC	3675	0.0	29.291302	94
TAGGCAT	4500	0.0	29.23863	5
GCATCGT	3910	0.0	28.81923	8
ATCAACG	7570	0.0	28.374681	3
CTAGGCA	5095	0.0	27.301851	4
TATCAAC	9120	0.0	26.38775	2
TATTCTC	2140	0.0	26.130316	5
CGTTAAC	795	0.0	26.112791	1
AACGCAG	9125	0.0	25.467548	6
TCTAACG	1705	0.0	25.36242	2
AGGGCTA	4125	0.0	25.289513	5
GTACATG	16195	0.0	24.267878	1
CTATTGA	4365	0.0	24.202328	9
CTGTGCG	4910	0.0	23.906576	9
CTAACGC	1755	0.0	23.835463	3
TACATGG	16210	0.0	23.777054	2
>>END_MODULE