FastQCFastQC Report
Mon 16 Jan 2017
ERR1146419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966131
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC118390.2985024952529304No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA96630.24363794337605085No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT94960.23942729072741167No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC83100.2095240928753992No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC82250.20738094631770865No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA77070.1943203590602529No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71220.17957046804555876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62630.15791208106842664No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC59740.15062538277227858No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC52060.1312614232863211No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG49140.12389908452343101No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT48650.12266362356664468No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG46740.11784784718406932No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG45490.11469616106981842No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA44860.11310771126823596No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC44600.11245216055647178No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA41770.10531674319380778No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT41370.10430820363724748No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT39810.10037489936666237No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128950.039.711941
GGTATCA75050.039.3480831
CAACGCA147600.033.4008335
ATCAACG151400.032.573593
GTACTAG36450.030.9619481
TATCAAC165550.030.812542
GTGGTAT25400.030.3616051
AACGCAG168850.029.9205866
GGGCACC37950.029.00458194
TCAACGC180000.027.6588424
ACGCAGA179300.027.3642087
TATTCTC22600.027.0335485
TAGGCAT46600.027.0281935
CGCAGAG181800.026.9879118
TACCCCG3850.026.8553055
CTAGGCA52300.024.8993844
GCAGAGT196000.024.7016499
AGGGCTA43650.024.548155
GTACATG161450.023.2714441
CTATTGA46350.022.8189
Produced by FastQC (version 0.11.5)