##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146418_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4118766
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.185124622277645	31.0	30.0	34.0	26.0	34.0
2	30.331300442899646	31.0	30.0	34.0	26.0	34.0
3	30.526479047365157	31.0	30.0	34.0	26.0	34.0
4	33.985614137826715	37.0	35.0	37.0	28.0	37.0
5	34.19398747100467	37.0	35.0	37.0	30.0	37.0
6	34.32034594827674	37.0	35.0	37.0	30.0	37.0
7	34.15245949879163	37.0	35.0	37.0	30.0	37.0
8	34.118262120256404	37.0	35.0	37.0	29.0	37.0
9	35.45264601096542	39.0	35.0	39.0	28.0	39.0
10-11	35.36320320212413	39.0	35.0	39.0	28.5	39.0
12-13	35.32996812637572	39.0	35.0	39.0	28.0	39.0
14-15	36.28981896519491	39.0	36.0	41.0	27.0	41.0
16-17	36.127160173702514	39.0	35.0	41.0	27.0	41.0
18-19	36.082553123921095	39.0	35.0	41.0	27.0	41.0
20-21	36.40909983718424	39.0	35.0	41.0	27.5	41.0
22-23	36.12835810531601	39.0	35.0	41.0	27.0	41.0
24-25	36.22708998277639	39.0	35.0	41.0	27.0	41.0
26-27	36.28221923750949	39.0	35.0	41.0	27.5	41.0
28-29	36.13056034258805	39.0	35.0	41.0	27.5	41.0
30-31	36.07116306194622	39.0	35.0	41.0	27.0	41.0
32-33	36.16641452804068	39.0	35.0	41.0	28.0	41.0
34-35	36.21429112506027	39.0	35.0	41.0	28.0	41.0
36-37	36.13894343597087	39.0	35.0	41.0	28.0	41.0
38-39	35.96102582666751	39.0	35.0	41.0	27.0	41.0
40-41	35.91808954429555	39.0	34.0	41.0	27.0	41.0
42-43	35.760321659448486	39.0	34.0	41.0	27.0	41.0
44-45	35.562855962198384	38.0	34.0	40.0	26.5	41.0
46-47	35.42099186989501	38.0	33.5	40.0	26.5	41.0
48-49	35.27179354204633	38.0	33.0	40.0	26.0	41.0
50-51	35.0444080824208	38.0	33.0	40.0	26.0	41.0
52-53	34.84175005329266	37.5	33.0	40.0	26.0	41.0
54-55	34.64460641852438	37.0	33.0	40.0	26.0	41.0
56-57	34.47194620913157	36.5	33.0	40.0	26.0	41.0
58-59	34.18773669103804	36.0	32.5	39.5	25.0	41.0
60-61	33.93420990655939	35.5	32.0	39.0	25.0	41.0
62-63	33.64972724840401	35.0	32.0	39.0	25.0	41.0
64-65	33.355633094960965	35.0	32.0	38.5	24.0	40.5
66-67	33.0766995503022	35.0	31.0	38.0	24.0	40.0
68-69	32.78975219762424	35.0	31.0	37.0	23.0	40.0
70-71	32.76007061338275	35.0	31.0	37.0	24.0	40.0
72-73	32.57798646487807	35.0	31.0	36.5	24.0	40.0
74-75	32.329721086364216	35.0	31.0	36.0	24.0	39.0
76-77	32.12166750915201	35.0	31.0	36.0	23.0	39.0
78-79	31.922886126572863	35.0	31.0	35.0	23.0	39.0
80-81	31.734378088000142	35.0	30.5	35.0	23.0	37.5
82-83	31.504829844667068	34.0	30.0	35.0	23.0	37.0
84-85	31.339163720395867	34.0	30.0	35.0	22.5	36.5
86-87	31.186781793381805	34.0	30.0	35.0	22.0	36.0
88-89	31.213405422886368	34.0	31.0	35.0	22.5	36.0
90-91	31.14722346450369	34.0	31.0	35.0	22.0	36.0
92-93	31.00899577689046	34.0	30.0	35.0	20.5	35.5
94-95	30.99612347484659	34.0	30.0	35.0	21.0	35.0
96-97	30.911101407557506	34.0	30.0	35.0	20.0	35.0
98-99	30.83986210918513	34.0	30.0	35.0	20.0	35.0
100	30.324149514684738	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7268541096780723
1101	2	0.8892135168721147
1101	3	0.8317522070770096
1101	4	0.8996788699722842
1101	5	0.7772558078841101
1101	6	0.5484492908991072
1101	7	0.576852048199278
1101	8	0.7311634404944272
1101	9	0.8715346433605831
1101	10-11	0.9158691833588151
1101	12-13	0.7358461419745268
1101	14-15	1.0024446013813133
1101	16-17	1.0671799482594722
1101	18-19	1.253193349821693
1101	20-21	0.8889890995079881
1101	22-23	1.1720604632626603
1101	24-25	1.0804211744314642
1101	26-27	0.9810122755105084
1101	28-29	1.2176111266157719
1101	30-31	1.1736619626212033
1101	32-33	1.0075359325638473
1101	34-35	0.8758209866692681
1101	36-37	0.8343483985821933
1101	38-39	0.9564784964593684
1101	40-41	0.862347658818841
1101	42-43	0.8487751427588393
1101	44-45	0.8979274681183043
1101	46-47	0.9137352255869473
1101	48-49	0.8719047268471982
1101	50-51	0.8209057165164282
1101	52-53	0.8832464058899063
1101	54-55	0.8773206447192337
1101	56-57	0.6925461985148544
1101	58-59	0.7002575264926207
1101	60-61	0.7229733313504809
1101	62-63	0.6292092079780502
1101	64-65	0.5869240924470205
1101	66-67	0.6902080058000166
1101	68-69	0.6621046142932556
1101	70-71	0.6098989682443872
1101	72-73	0.5972046732097311
1101	74-75	0.6134746141900536
1101	76-77	0.6305624402177799
1101	78-79	0.5699728745085793
1101	80-81	0.6419123155882644
1101	82-83	0.5746602545851687
1101	84-85	0.584113934975786
1101	86-87	0.5995554684866491
1101	88-89	0.6946446893536944
1101	90-91	0.6537244738240666
1101	92-93	0.6815065601188763
1101	94-95	0.6555914365004014
1101	96-97	0.6640987507750111
1101	98-99	0.634600889911809
1101	100	0.6985524418667914
1102	1	0.6624433987495948
1102	2	0.825218351293195
1102	3	0.7734083723067791
1102	4	0.9362172096172188
1102	5	0.8193609296846631
1102	6	0.5954095680725828
1102	7	0.6569962213789893
1102	8	0.6555013128119924
1102	9	0.8596978319298643
1102	10-11	0.8034118471248277
1102	12-13	0.7392331910976182
1102	14-15	1.0222385476118063
1102	16-17	1.1985784388457859
1102	18-19	1.252902210241217
1102	20-21	0.9624661762947042
1102	22-23	1.2708000759718132
1102	24-25	1.1014707809633109
1102	26-27	0.9006531854559938
1102	28-29	1.033841298774874
1102	30-31	1.110865142595955
1102	32-33	0.9633973458262801
1102	34-35	0.7892272660450885
1102	36-37	0.8168825483955331
1102	38-39	0.7993406886007506
1102	40-41	0.7718811371848133
1102	42-43	0.8368640423908928
1102	44-45	0.9001904069091609
1102	46-47	0.911031333877979
1102	48-49	0.853585923634995
1102	50-51	0.8836023565937765
1102	52-53	0.8747889622243363
1102	54-55	0.8989421095488268
1102	56-57	0.8116589210996565
1102	58-59	0.7869909081701607
1102	60-61	0.7635763448062818
1102	62-63	0.6867317820386916
1102	64-65	0.6602934153594902
1102	66-67	0.6772677646161327
1102	68-69	0.7030354014981555
1102	70-71	0.7011248699982104
1102	72-73	0.6713705475685003
1102	74-75	0.6907150192071043
1102	76-77	0.7076967565500567
1102	78-79	0.6568871172502284
1102	80-81	0.7516425548921077
1102	82-83	0.7306127161112386
1102	84-85	0.6899771745750662
1102	86-87	0.6948618902093102
1102	88-89	0.6473431557678957
1102	90-91	0.6335525832968472
1102	92-93	0.7175852537508689
1102	94-95	0.7096773280482225
1102	96-97	0.6854519052347641
1102	98-99	0.734596646364416
1102	100	0.7132270584703413
1103	1	0.5580757274345558
1103	2	0.7134288776089832
1103	3	0.6320360526186697
1103	4	0.7093502130660099
1103	5	0.676236023401863
1103	6	0.47222828152287377
1103	7	0.5458035388178857
1103	8	0.6624744722062061
1103	9	0.633061154766871
1103	10-11	0.7251740303606979
1103	12-13	0.6393336559349265
1103	14-15	0.7302481442529825
1103	16-17	1.0147607750281225
1103	18-19	1.043968337096814
1103	20-21	0.7925912325250266
1103	22-23	0.9821666976542289
1103	24-25	0.8834440903050336
1103	26-27	0.8139280392571635
1103	28-29	0.9409317171284499
1103	30-31	0.9822704507381061
1103	32-33	0.7683180235890603
1103	34-35	0.6946691613817251
1103	36-37	0.7033060001874034
1103	38-39	0.8081300123374433
1103	40-41	0.7488521973137665
1103	42-43	0.7526532161800645
1103	44-45	0.7666909317254778
1103	46-47	0.7019867994650255
1103	48-49	0.6889063614817417
1103	50-51	0.8149095990589146
1103	52-53	0.8487172873105564
1103	54-55	0.6984358867268128
1103	56-57	0.6136934090288833
1103	58-59	0.6406733193262539
1103	60-61	0.6548050147717888
1103	62-63	0.6602459520791797
1103	64-65	0.6284175719047056
1103	66-67	0.6569456773466698
1103	68-69	0.6883303482667813
1103	70-71	0.5917828397088059
1103	72-73	0.5623626003500277
1103	74-75	0.6426034582534399
1103	76-77	0.6286885094365502
1103	78-79	0.6258398837819428
1103	80-81	0.6350286697519039
1103	82-83	0.5963716005016977
1103	84-85	0.5616733809028531
1103	86-87	0.5766294715558402
1103	88-89	0.5920805976632302
1103	90-91	0.5172952733553267
1103	92-93	0.5913483366718495
1103	94-95	0.5787371571080513
1103	96-97	0.6035796605204169
1103	98-99	0.6113988655876881
1103	100	0.6614441824769379
1104	1	0.4685213886337536
1104	2	0.6195267213372446
1104	3	0.5778965432971539
1104	4	0.6345503797917971
1104	5	0.5634301089961369
1104	6	0.2652030090906905
1104	7	0.31338323394221135
1104	8	0.3522975275289113
1104	9	0.47457138389592757
1104	10-11	0.519884681192373
1104	12-13	0.34695386331168265
1104	14-15	0.5785211385728175
1104	16-17	0.6695394439037017
1104	18-19	0.6751848012022208
1104	20-21	0.615666372085208
1104	22-23	0.8271410777665622
1104	24-25	0.6605717762317056
1104	26-27	0.5206613433936695
1104	28-29	0.7368395040285876
1104	30-31	0.780090464834494
1104	32-33	0.5653317560073887
1104	34-35	0.463353877237779
1104	36-37	0.4771090127453377
1104	38-39	0.5242967988960672
1104	40-41	0.4940250432053688
1104	42-43	0.4545941778126519
1104	44-45	0.5696914674410607
1104	46-47	0.5272104821892896
1104	48-49	0.45863275019723915
1104	50-51	0.5649709322178609
1104	52-53	0.643315312363022
1104	54-55	0.5032387321626288
1104	56-57	0.4522590189949156
1104	58-59	0.43044465266355303
1104	60-61	0.40392778876124424
1104	62-63	0.408163663259117
1104	64-65	0.38098520847419337
1104	66-67	0.4956316086363586
1104	68-69	0.45120182342668613
1104	70-71	0.29575988386670105
1104	72-73	0.28848035340958234
1104	74-75	0.2908625147202457
1104	76-77	0.3943183914007662
1104	78-79	0.3026753371882265
1104	80-81	0.3230786782401225
1104	82-83	0.3155916686320417
1104	84-85	0.3542734708713624
1104	86-87	0.22881979525091722
1104	88-89	0.17111767037060588
1104	90-91	0.31157183602975635
1104	92-93	0.2836916332581296
1104	94-95	0.2536665595704939
1104	96-97	0.2874430946620805
1104	98-99	0.4114858615708137
1104	100	0.474095063038785
1105	1	0.43354964449519073
1105	2	0.5818446443533531
1105	3	0.5669726542747959
1105	4	0.7282613103615319
1105	5	0.6399282580856749
1105	6	0.2837573301160319
1105	7	0.42993108574018635
1105	8	0.4871764938683114
1105	9	0.4406249295607907
1105	10-11	0.5587644633747075
1105	12-13	0.4930779457649166
1105	14-15	0.6941029199685005
1105	16-17	0.8183851633165631
1105	18-19	0.8025823905721907
1105	20-21	0.5632172726758071
1105	22-23	0.87359607655209
1105	24-25	0.7890686741261277
1105	26-27	0.5462376430681672
1105	28-29	0.8520526297201627
1105	30-31	0.7732746590723636
1105	32-33	0.6367190960187727
1105	34-35	0.5330812158068454
1105	36-37	0.481375043903995
1105	38-39	0.6192951818104362
1105	40-41	0.4906478339810363
1105	42-43	0.4195065830936855
1105	44-45	0.5082002269129546
1105	46-47	0.4536391474764514
1105	48-49	0.48632842751278105
1105	50-51	0.5906239494430352
1105	52-53	0.45210537836931763
1105	54-55	0.4265781444991035
1105	56-57	0.32220801553908274
1105	58-59	0.3762019155016887
1105	60-61	0.5156280337353465
1105	62-63	0.40218692805545686
1105	64-65	0.33834894301730856
1105	66-67	0.4362552329521918
1105	68-69	0.41791565324734137
1105	70-71	0.33666765954883004
1105	72-73	0.41974859539638487
1105	74-75	0.3608905294567393
1105	76-77	0.3791990832214154
1105	78-79	0.39665413766020663
1105	80-81	0.4648850978267234
1105	82-83	0.426241051285821
1105	84-85	0.4758161041946529
1105	86-87	0.4433143022477424
1105	88-89	0.4185209759502371
1105	90-91	0.4595974651778221
1105	92-93	0.5231726350459951
1105	94-95	0.41213422604293015
1105	96-97	0.3869867048732125
1105	98-99	0.41919807696042355
1105	100	0.5051692989934793
1106	1	0.4285888727434468
1106	2	0.5110173885976472
1106	3	0.47797221774066045
1106	4	0.6506722169711452
1106	5	0.4538541774450593
1106	6	0.2827292632189895
1106	7	0.2979269607826538
1106	8	0.3510774593556505
1106	9	0.5028098789949809
1106	10-11	0.4160237604822328
1106	12-13	0.3267208231339893
1106	14-15	0.4933902390144027
1106	16-17	0.6229894563614948
1106	18-19	0.5276441543513926
1106	20-21	0.3880831036914145
1106	22-23	0.7207685209510899
1106	24-25	0.5617502300875259
1106	26-27	0.38320666854196617
1106	28-29	0.5638788706427675
1106	30-31	0.5832972190767762
1106	32-33	0.4122098207560825
1106	34-35	0.36482687829271754
1106	36-37	0.3209827623278727
1106	38-39	0.44008724562708323
1106	40-41	0.2961952166071953
1106	42-43	0.28542574850133207
1106	44-45	0.3219241948487266
1106	46-47	0.3573225492811076
1106	48-49	0.3750018214823001
1106	50-51	0.42540380063710614
1106	52-53	0.3886909544692898
1106	54-55	0.3604956594886488
1106	56-57	0.35588615736119067
1106	58-59	0.2909524003482602
1106	60-61	0.35834188832814107
1106	62-63	0.26768592029246463
1106	64-65	0.33113443688643684
1106	66-67	0.3455893316705456
1106	68-69	0.3800626778719973
1106	70-71	0.3802103760819122
1106	72-73	0.33188044193940414
1106	74-75	0.38608091036902437
1106	76-77	0.4334159703486975
1106	78-79	0.4311364821385837
1106	80-81	0.47811427571039644
1106	82-83	0.412727437330652
1106	84-85	0.37078971635872904
1106	86-87	0.4438494553918204
1106	88-89	0.4651548953052682
1106	90-91	0.41215834609936053
1106	92-93	0.4650937441907672
1106	94-95	0.4474310384373119
1106	96-97	0.45463782581238377
1106	98-99	0.44449489479335114
1106	100	0.3278225926326641
1107	1	0.39972731243141624
1107	2	0.599250762163301
1107	3	0.4574480409956614
1107	4	0.5874957491889532
1107	5	0.48993629188306187
1107	6	0.2597599378889157
1107	7	0.3196621124008985
1107	8	0.34802974850273216
1107	9	0.42904767541583766
1107	10-11	0.40057165764589797
1107	12-13	0.2865217586479716
1107	14-15	0.4606117989484133
1107	16-17	0.5760132708355883
1107	18-19	0.8063280669131387
1107	20-21	0.471194506719371
1107	22-23	0.784644891617674
1107	24-25	0.43444620964554304
1107	26-27	0.39787554618620646
1107	28-29	0.6272041620120916
1107	30-31	0.5855470451417304
1107	32-33	0.45871954311706276
1107	34-35	0.3221376513743195
1107	36-37	0.3069991167215207
1107	38-39	0.3568779056981697
1107	40-41	0.19035034685820307
1107	42-43	0.3344002068275742
1107	44-45	0.18872283163007353
1107	46-47	0.25953744208042906
1107	48-49	0.21514419394718942
1107	50-51	0.29000275549679344
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2216	100	-0.8497843641672489
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	17111.0
1	2708.0
2	481.0
3	406.0
4	362.0
5	203.0
6	210.0
7	255.0
8	476.0
9	909.0
10	1677.0
11	3761.0
12	6941.0
13	9960.0
14	12597.0
15	15353.0
16	18033.0
17	21069.0
18	23916.0
19	26939.0
20	30364.0
21	33619.0
22	39023.0
23	44017.0
24	49551.0
25	55856.0
26	62421.0
27	71547.0
28	83550.0
29	97665.0
30	114220.0
31	136040.0
32	164487.0
33	206427.0
34	281426.0
35	375239.0
36	546500.0
37	787840.0
38	578091.0
39	197399.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05345654304742	21.975704157395977	12.41078253389007	23.56005676566653
2	15.277526773700496	20.01090678769494	41.01759451187613	23.693971926728434
3	18.29194268025623	27.780401831546158	28.810524559191496	25.117130929006116
4	12.065971396392605	17.069346269819604	41.023458724456404	29.841223609331387
5	12.970144099977468	38.931525930290064	35.01554832221508	13.082781647517388
6	28.74879168827924	39.252015398075585	18.661700531925536	13.33749238171964
7	26.176597695304103	31.87913755578662	24.612388393211194	17.331876355698085
8	25.773726947174485	35.023749824606355	20.329508226724503	18.873015001494657
9	26.05345900053766	14.88874728088636	22.353267927357535	36.704525791218444
10-11	25.66416870870113	25.954357692186587	27.405949453880368	20.975524145231915
12-13	25.8728564402293	23.90153202509724	27.400426911797194	22.82518462287627
14-15	23.423178681784126	24.82342806032095	24.981166025890506	26.77222723200442
16-17	22.082633900031954	27.26708535433655	26.529089143175998	24.121191602455497
18-19	21.42690462445735	28.329454294261012	28.112480335285667	22.131160745995963
20-21	23.369549563887002	27.34676487487777	26.742596916575906	22.541088644659318
22-23	24.07506995474292	26.896122452764075	26.38184407241524	22.64696352007777
24-25	23.391767546678338	27.087457765126878	27.217356772431927	22.30341791576286
26-27	23.150745890400678	27.310988813748803	26.179197943458533	23.35906735239199
28-29	22.126999707385192	27.964738329391537	26.853107122644655	23.05515484057862
30-31	23.128002869111665	27.396245773728474	26.74943876480789	22.72631259235197
32-33	23.627330432566946	27.77430937581456	25.58435929151531	23.01400090010319
34-35	23.532070930667523	27.389162441334037	26.817645648115885	22.261120979882556
36-37	23.179070595517732	26.999460092827903	26.471771936765307	23.349697374889054
38-39	23.444602381951476	26.91181887619286	27.1083863839805	22.53519235787516
40-41	22.921399580207964	26.02285859921871	27.88488626604308	23.17085555453024
42-43	22.90048840242583	26.361342119222726	27.023249830278512	23.71491964807294
44-45	22.98884319276312	27.511758672047335	26.849164170238936	22.650233964950605
46-47	22.969990241939836	27.056571603092795	26.32985612415319	23.64358203081418
48-49	23.819766476945457	26.50186466987533	26.729566732727395	22.948802120451816
50-51	23.363325684714155	26.377178635942002	26.563076697749356	23.696418981594494
52-53	22.62027894969945	27.122825812226232	26.443349227044276	23.813546011030038
54-55	22.70189621055705	27.025809437649734	26.362887606597457	23.90940674519576
56-57	23.96378343283655	26.343500886009828	26.08178627392593	23.610929407227694
58-59	22.647066254034108	26.763904716003488	27.246905469399234	23.34212356056317
60-61	22.80956451232615	27.553756814029175	26.289057866344223	23.34762080730045
62-63	23.323437305891954	26.66610450331892	25.742244769501234	24.2682134212879
64-65	22.192487719719626	26.913695331882447	26.475256858259545	24.41856009013838
66-67	23.31214180485137	26.29237670044106	26.338485391734096	24.056996102973475
68-69	22.399265930424274	26.53868543978563	26.687193662119256	24.374854967670835
70-71	22.41854686297639	26.128224577608023	26.843644028234525	24.60958453118106
72-73	23.205502718966713	26.32599821363753	27.21766599708587	23.25083307030989
74-75	23.341356041013448	26.721804474571638	26.733948380466742	23.202891103948172
76-77	22.9377996736768	26.697948459483566	26.28644440729138	24.077807459548247
78-79	22.83510349024364	26.70026923149697	26.366876285960352	24.097750992299037
80-81	22.364305215586263	27.07249328438182	27.184392794740614	23.378808705291302
82-83	23.411414503138854	25.7030191572789	28.353097404425654	22.53246893515659
84-85	23.65944467803739	26.527739551936865	26.59253667493972	23.220279095086024
86-87	22.034223669796386	27.240544613050595	27.262556946813405	23.462674770339618
88-89	23.283624633003416	25.70795953398109	27.022671817375304	23.98574401564019
90-91	22.93111301857271	25.807133047164992	27.246493259504508	24.01526067475779
92-93	23.250033202707378	26.125166719324568	27.108887450309478	23.51591262765858
94-95	21.924946545492887	26.1105212979033	27.879899546402342	24.084632610201474
96-97	22.315696861825593	26.08622434068978	27.304165890836707	24.293912906647925
98-99	21.60089892770591	26.939135205526615	27.147088948307363	24.312876918460113
100	22.589141372858883	25.986309998080475	26.174408837478968	25.250139791581677
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14165.0
1	8333.0
2	2268.5
3	1543.5
4	796.5
5	430.5
6	295.0
7	262.5
8	279.5
9	277.5
10	238.5
11	232.0
12	228.0
13	235.5
14	245.0
15	298.0
16	357.0
17	408.5
18	543.0
19	659.0
20	782.0
21	967.5
22	1272.0
23	1786.5
24	2428.0
25	3241.0
26	4858.0
27	6724.5
28	10217.5
29	18809.5
30	30831.0
31	42257.5
32	57131.5
33	71814.0
34	83771.5
35	96323.0
36	107534.5
37	128909.5
38	137427.0
39	125888.0
40	113604.5
41	101279.5
42	98731.0
43	106180.0
44	119370.5
45	146775.0
46	173079.5
47	190999.0
48	202011.0
49	197247.0
50	187486.0
51	192910.5
52	197368.5
53	189135.5
54	177995.0
55	153376.0
56	133030.0
57	108015.5
58	82314.5
59	77642.0
60	64544.0
61	44219.0
62	32802.0
63	27110.0
64	19478.5
65	8750.5
66	4289.5
67	2767.5
68	1949.0
69	1466.0
70	1132.0
71	860.5
72	661.0
73	556.5
74	447.0
75	329.0
76	236.5
77	173.5
78	118.5
79	61.5
80	45.0
81	39.5
82	28.5
83	25.5
84	19.5
85	16.0
86	14.5
87	11.5
88	9.0
89	8.5
90	8.5
91	7.0
92	8.0
93	8.0
94	5.0
95	3.5
96	3.0
97	2.0
98	0.5
99	3.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9942055460300488
2	0.4507903580829792
3	0.4839799104877529
4	0.4698737437378088
5	0.43707265719878235
6	0.48733042857982223
7	0.5046414387221804
8	0.5060010692522955
9	0.5191603504544808
10-11	0.5329994469217236
12-13	0.5317369328580454
14-15	0.5288355784232461
16-17	0.5340677280525283
18-19	0.5331936798545972
20-21	0.5315184208085626
22-23	0.4953425370608575
24-25	0.5277551577341368
26-27	0.5101649377507729
28-29	0.5154213664966643
30-31	0.517885696832498
32-33	0.5209812842001706
34-35	0.5328901908969823
36-37	0.5509295745376164
38-39	0.5392149007736783
40-41	0.5451147261097135
42-43	0.542613977098966
44-45	0.5522649259511223
46-47	0.5502861779474727
48-49	0.5226322641295961
50-51	0.5381708987594828
52-53	0.5308021868685912
54-55	0.5409387180529314
56-57	0.5279736697836197
58-59	0.5336914017450858
60-61	0.5269539468860334
62-63	0.5219767279811477
64-65	0.5330237260383328
66-67	0.5323074920983615
68-69	0.5510509701206623
70-71	0.5343469378935342
72-73	0.5383408525757473
74-75	0.535621591515517
76-77	0.5244531978752859
78-79	0.5547413958452605
80-81	0.5289569740062922
82-83	0.5295639519215222
84-85	0.5373818274696839
86-87	0.8422911134062969
88-89	1.1088151159837678
90-91	1.0995890516722726
92-93	1.0999168197464968
94-95	1.10330375651348
96-97	1.0967241159123873
98-99	1.093130806654226
100	1.088772705222875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4118766.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.638860006667333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5099784977872	17.84969810815652
2	11.470593082475459	5.423034881401682
3	4.6536071358327185	3.3001790282993335
4	2.3705767912694165	2.2415093161548953
5	1.3878560194840603	1.6403667076997137
6	0.8746211436051772	1.2405028063532442
7	0.6034358324373276	0.9985174616194927
8	0.40750263922760754	0.7706318272839102
9	0.31066423551970046	0.6609373535275677
>10	1.73831089859232	7.862433272712711
>50	0.23760195867093425	4.005866076854413
>100	0.31750940138080036	16.863138624596736
>500	0.0644755127080931	10.721715170516879
>1k	0.05018189824808518	20.70134791765816
>5k	0.0023651304502171296	3.455831757207701
>10k+	7.198223109356482E-4	2.264289689957164
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	16935	0.41116683977676804	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	14081	0.341874240974117	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	13782	0.3346147851079668	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12716	0.30873324680256176	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	12435	0.3019108150353771	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	11890	0.2886786964833642	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	11310	0.27459680885002935	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9767	0.23713413192203683	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7878	0.19127088064726183	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	7482	0.1816563504700194	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7324	0.17782025004576615	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6864	0.16665185640553504	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6747	0.16381119976225889	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	6583	0.15982942463835043	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6531	0.15856691057467212	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6057	0.14705860930191225	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5915	0.14361097474340614	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5826	0.14145013336518752	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5790	0.14057608516725642	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5769	0.14006622371846325	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	5608	0.13615728594438237	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5554	0.13484621364748567	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5523	0.13409356103260053	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5457	0.13249113933639348	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5443	0.1321512317038647	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5268	0.12790238629725503	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	5258	0.12765959513116307	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5255	0.1275867577813355	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5255	0.1275867577813355	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5013	0.12171121156190957	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	4851	0.11777799467121948	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	4842	0.1175594826217367	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4834	0.11736524968886312	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4720	0.11459743039541453	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4498	0.10920746650817259	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4454	0.10813918537736789	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4356	0.10575983194966647	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	4333	0.10520141226765492	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	4329	0.10510429580121813	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	4298	0.104351643186333	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	4294	0.1042545267198962	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	4233	0.10277350060673511	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	4130	0.10027275159598774	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.4279116609197998E-5
6	0.0	0.0	0.0	0.0	2.4279116609197998E-5
7	0.0	0.0	0.0	0.0	2.4279116609197998E-5
8	0.0	0.0	0.0	0.0	2.4279116609197998E-5
9	0.0	0.0	0.0	0.0	2.4279116609197998E-5
10-11	0.0	0.0	0.0	0.0	3.6418674913797E-5
12-13	0.0	0.0	0.0	0.0	3.03488957614975E-4
14-15	0.0	0.0	0.0	0.0	3.3990763252877195E-4
16-17	0.0	0.0	0.0	0.0	3.6418674913797E-4
18-19	0.0	0.0	0.0	0.0	3.6418674913797E-4
20-21	0.0	0.0	0.0	1.2139558304598999E-5	4.2488454066096494E-4
22-23	0.0	0.0	0.0	8.497690813219299E-5	4.37024098965564E-4
24-25	0.0	0.0	0.0	1.9423293287358398E-4	4.37024098965564E-4
26-27	0.0	0.0	0.0	5.22001007097757E-4	4.6130321557476196E-4
28-29	0.0	0.0	0.0	0.0015174447880748748	4.6130321557476196E-4
30-31	0.0	0.0	0.0	0.005790569311293722	4.6130321557476196E-4
32-33	0.0	0.0	0.0	0.01659477620238683	4.6130321557476196E-4
34-35	0.0	0.0	0.0	0.033529460037302436	5.098614487931579E-4
36-37	0.0	0.0	0.0	0.06448533371402988	5.098614487931579E-4
38-39	0.0	0.0	0.0	0.11562929285130547	5.82698798620752E-4
40-41	0.0	0.0	0.0	0.17508884942723135	5.82698798620752E-4
42-43	0.0	0.0	0.0	0.23920999639212326	5.948383569253509E-4
44-45	0.0	0.0	0.0	0.31550712033652795	6.0697791522995E-4
46-47	0.0	0.0	0.0	0.41149460785099223	6.433965901437469E-4
48-49	0.0	0.0	0.0	0.5306686517272406	6.798152650575439E-4
50-51	0.0	0.0	0.0	0.6568472207452426	6.798152650575439E-4
52-53	0.0	0.0	0.0	0.7839362566360896	7.040943816667419E-4
54-55	0.0	0.0	0.0	0.9269402534642657	7.40513056580539E-4
56-57	0.0	0.0	0.0	1.1021140797996294	7.52652614885138E-4
58-59	0.0	0.0	0.0	1.3157096081690487	7.52652614885138E-4
60-61	0.0	0.0	0.0	1.5404128323871762	7.52652614885138E-4
62-63	0.0	0.0	0.0	1.776369427153667	7.52652614885138E-4
64-65	0.0	0.0	0.0	2.0274033533344697	7.52652614885138E-4
66-67	0.0	0.0	0.0	2.312780089958983	7.52652614885138E-4
68-69	0.0	0.0	0.0	2.6347454553135576	7.647921731897369E-4
70-71	0.0	0.0	0.0	2.9945741030201765	8.012108481035339E-4
72-73	0.0	0.0	0.0	3.3768366544736943	8.619086396265289E-4
74-75	0.0	0.0	0.0	3.7515484006617514	9.10466872844925E-4
76-77	0.0	0.0	0.0	4.183668118072258	9.468855477587219E-4
78-79	0.0	0.0	0.0	4.649596505361072	9.711646643679199E-4
80-81	0.0	0.0	0.0	5.149940540443424	0.001031862455890915
82-83	0.0	0.0	0.0	5.682357288566527	0.001116839364023108
84-85	0.0	0.0	0.0	6.200740707289514	0.001116839364023108
86-87	0.0	0.0	0.0	6.758674806968884	0.001165397597241504
88	0.0	0.0	0.0	7.206624508408586	0.0012139558304599
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4630	0.0	38.127617	94
GTACTAG	4870	0.0	34.180153	1
CAACGCA	7860	0.0	33.29445	5
GTATCAA	9390	0.0	31.336266	1
TATTCTC	2220	0.0	30.89862	5
GCATCGT	5100	0.0	30.65643	8
TAGGCAT	6130	0.0	30.427706	5
ATCAACG	8760	0.0	30.091263	3
AGGGCTA	5360	0.0	30.065506	5
TACCCCG	390	0.0	27.707823	5
CTAGGCA	7015	0.0	27.593624	4
TAGTACT	6865	0.0	27.580599	4
CTGTGCG	6850	0.0	27.555578	9
CTATTGA	5820	0.0	27.107555	9
ACGCAGA	9780	0.0	26.788158	7
AACGCAG	10560	0.0	26.719374	6
GCTATTG	5895	0.0	26.681396	8
TATCAAC	11085	0.0	26.577784	2
CGCAGAG	9830	0.0	26.413088	8
ACTGTGC	7320	0.0	25.977148	8
>>END_MODULE