##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146417_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4148838
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.550392423131488	31.0	30.0	34.0	27.0	34.0
2	30.679315750578837	31.0	31.0	34.0	27.0	34.0
3	30.86443529489462	31.0	31.0	34.0	27.0	34.0
4	34.333368041846896	37.0	35.0	37.0	30.0	37.0
5	34.57383151619803	37.0	35.0	37.0	30.0	37.0
6	34.67522038700957	37.0	35.0	37.0	31.0	37.0
7	34.56974434769446	37.0	35.0	37.0	30.0	37.0
8	34.517263628996844	37.0	35.0	37.0	30.0	37.0
9	35.8959489379918	39.0	35.0	39.0	30.0	39.0
10-11	35.82089539287868	39.0	35.0	39.0	30.0	39.0
12-13	35.81190468270875	39.0	35.0	39.0	30.0	39.0
14-15	36.83034623188469	40.0	36.0	41.0	30.0	41.0
16-17	36.66332645429877	40.0	36.0	41.0	29.5	41.0
18-19	36.604935647041415	40.0	36.0	41.0	28.5	41.0
20-21	36.95105569318446	40.0	36.0	41.0	30.0	41.0
22-23	36.66218505036832	40.0	36.0	41.0	29.0	41.0
24-25	36.77728318145948	40.0	36.0	41.0	30.0	41.0
26-27	36.85983400653388	40.0	36.0	41.0	30.0	41.0
28-29	36.691817323308356	40.0	35.5	41.0	29.5	41.0
30-31	36.63458491269122	39.0	35.0	41.0	29.5	41.0
32-33	36.73991440494905	40.0	35.5	41.0	30.0	41.0
34-35	36.79026814254979	40.0	35.0	41.0	30.0	41.0
36-37	36.710372277731736	39.5	35.0	41.0	30.0	41.0
38-39	36.54044047996089	39.0	35.0	41.0	29.5	41.0
40-41	36.495320255936726	39.0	35.0	41.0	29.0	41.0
42-43	36.329409102018445	39.0	35.0	41.0	29.0	41.0
44-45	36.13201431340535	39.0	35.0	41.0	28.0	41.0
46-47	36.00093532213116	38.5	35.0	40.5	28.0	41.0
48-49	35.86532771344651	38.0	34.0	40.0	28.0	41.0
50-51	35.636842894323664	38.0	34.0	40.0	27.5	41.0
52-53	35.43893687822952	38.0	34.0	40.0	27.0	41.0
54-55	35.242225654508566	38.0	34.0	40.0	27.0	41.0
56-57	35.08205442102102	37.0	33.0	40.0	27.0	41.0
58-59	34.80971274366461	37.0	33.0	40.0	26.5	41.0
60-61	34.547790971833564	36.0	33.0	39.5	26.0	41.0
62-63	34.27174657096758	35.5	33.0	39.0	26.0	41.0
64-65	33.99178420560166	35.0	32.5	39.0	26.0	41.0
66-67	33.69933942949809	35.0	32.0	38.5	26.0	40.5
68-69	33.4097938989182	35.0	32.0	38.0	25.0	40.0
70-71	33.35583385034556	35.0	32.0	37.5	25.5	40.0
72-73	33.154600034997756	35.0	32.0	37.0	25.0	40.0
74-75	32.88600024874435	35.0	32.0	36.5	25.0	39.0
76-77	32.657723560187215	35.0	32.0	36.0	25.0	39.0
78-79	32.43724266891115	35.0	32.0	36.0	25.0	39.0
80-81	32.223070893585145	35.0	31.0	35.5	24.0	37.5
82-83	31.988101487693662	35.0	31.0	35.0	24.0	37.0
84-85	31.786938535561042	35.0	31.0	35.0	24.0	37.0
86-87	31.61242593709371	34.5	31.0	35.0	24.0	36.0
88-89	31.625077310803654	35.0	31.0	35.0	24.0	36.0
90-91	31.539953596645617	35.0	31.0	35.0	24.0	36.0
92-93	31.394907080006497	35.0	31.0	35.0	23.0	36.0
94-95	31.360025746968187	35.0	31.0	35.0	23.0	35.0
96-97	31.25382998805931	35.0	31.0	35.0	23.0	35.0
98-99	31.162806670205008	35.0	31.0	35.0	23.0	35.0
100	30.64829935514474	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6871340911032533
1101	2	0.8749029768100307
1101	3	0.8349657444246184
1101	4	0.878061214068758
1101	5	0.8025625283284938
1101	6	0.610867636505219
1101	7	0.6367962402817824
1101	8	0.7137344456926868
1101	9	0.8651971411921622
1101	10-11	0.8789412260517651
1101	12-13	0.7651594998294016
1101	14-15	0.9857809970197309
1101	16-17	1.1544903582120867
1101	18-19	1.27361335181687
1101	20-21	0.9499058869878425
1101	22-23	1.2250461191850164
1101	24-25	1.0579643608280804
1101	26-27	0.9692865415619778
1101	28-29	1.1773895626284627
1101	30-31	1.1865175362792542
1101	32-33	1.020141810867841
1101	34-35	0.8418831066656978
1101	36-37	0.8020510675607468
1101	38-39	0.8851229289502314
1101	40-41	0.8636487589816753
1101	42-43	0.8703154481842077
1101	44-45	0.8911795154842963
1101	46-47	0.9271723031871701
1101	48-49	0.8609641829182664
1101	50-51	0.9281283941583354
1101	52-53	0.8768969652819791
1101	54-55	0.8534517012706857
1101	56-57	0.7553711734950426
1101	58-59	0.7173605723029155
1101	60-61	0.789677407936594
1101	62-63	0.7048857401068531
1101	64-65	0.6885464097757463
1101	66-67	0.677699805485247
1101	68-69	0.7108941218185691
1101	70-71	0.6233315663192229
1101	72-73	0.5788682874619653
1101	74-75	0.5832199368379349
1101	76-77	0.621482249694445
1101	78-79	0.5370301320661852
1101	80-81	0.6084844310553805
1101	82-83	0.6333645255350859
1101	84-85	0.6428366454282859
1101	86-87	0.6283328102297929
1101	88-89	0.6313532499891998
1101	90-91	0.5828897503920523
1101	92-93	0.5993253439237911
1101	94-95	0.5725041563856408
1101	96-97	0.5900978416516303
1101	98-99	0.5907116815504061
1101	100	0.6169921506406446
1102	1	0.6131594214278522
1102	2	0.7553145598923052
1102	3	0.7513505279887553
1102	4	0.8068464432454476
1102	5	0.6279149040681986
1102	6	0.5029912350967507
1102	7	0.5481370640079248
1102	8	0.6266422882450726
1102	9	0.7083710032965627
1102	10-11	0.754691728626085
1102	12-13	0.6791321791648457
1102	14-15	0.829211497256054
1102	16-17	1.0131951117509743
1102	18-19	1.0039397190257233
1102	20-21	0.7619618454032278
1102	22-23	1.047916613564574
1102	24-25	0.9015502537064037
1102	26-27	0.7400356150045369
1102	28-29	0.9118074906732829
1102	30-31	0.8969851381883132
1102	32-33	0.8065205939909532
1102	34-35	0.74977970813692
1102	36-37	0.7408904679167776
1102	38-39	0.7621636709983974
1102	40-41	0.7100843219583268
1102	42-43	0.7102084856768869
1102	44-45	0.8193479305102329
1102	46-47	0.758749946476577
1102	48-49	0.6889754204198582
1102	50-51	0.6858823104672638
1102	52-53	0.6321455123558692
1102	54-55	0.6723870204196629
1102	56-57	0.592425451227939
1102	58-59	0.6165961424975634
1102	60-61	0.6417343696127631
1102	62-63	0.6316324767265229
1102	64-65	0.52903158443997
1102	66-67	0.5979094104961717
1102	68-69	0.5847614386662343
1102	70-71	0.4760622230122564
1102	72-73	0.5170208145832262
1102	74-75	0.5341240140731287
1102	76-77	0.5601556903165417
1102	78-79	0.5553307048810225
1102	80-81	0.547452279194836
1102	82-83	0.538701071231678
1102	84-85	0.5603038081287899
1102	86-87	0.5702388738330981
1102	88-89	0.6142166877088933
1102	90-91	0.6767353215865306
1102	92-93	0.724764423756131
1102	94-95	0.6334985878959571
1102	96-97	0.6591293188854799
1102	98-99	0.6172620271727709
1102	100	0.5538711102660656
1103	1	0.5349840347632409
1103	2	0.7317428131595172
1103	3	0.702354439572332
1103	4	0.6020768938202536
1103	5	0.5677311651682828
1103	6	0.4284108950981462
1103	7	0.4832221140928894
1103	8	0.48871740773977734
1103	9	0.5366172574987758
1103	10-11	0.6907041942711487
1103	12-13	0.5825238581217249
1103	14-15	0.7030286231351681
1103	16-17	0.9869062765068932
1103	18-19	0.9765017306189776
1103	20-21	0.7246166989422207
1103	22-23	0.9452597636496947
1103	24-25	0.8661868766605565
1103	26-27	0.7126716104990862
1103	28-29	0.8741902690100005
1103	30-31	0.9535515638847301
1103	32-33	0.7126459899951385
1103	34-35	0.7437313457884187
1103	36-37	0.6613966928889212
1103	38-39	0.7451678798497596
1103	40-41	0.6341960131941491
1103	42-43	0.7162121246381119
1103	44-45	0.7493377497544884
1103	46-47	0.7450528143970914
1103	48-49	0.7345850206470175
1103	50-51	0.7839433803477505
1103	52-53	0.7770967462598577
1103	54-55	0.808952809521486
1103	56-57	0.6595693624289893
1103	58-59	0.6668447712261738
1103	60-61	0.6857280757951472
1103	62-63	0.6499021945305188
1103	64-65	0.5910517655354326
1103	66-67	0.6790429399716942
1103	68-69	0.6531327927467174
1103	70-71	0.5813417665444334
1103	72-73	0.6216944751629754
1103	74-75	0.6507233799716232
1103	76-77	0.6128279222219533
1103	78-79	0.5535140313742133
1103	80-81	0.6612336197032036
1103	82-83	0.589769517711531
1103	84-85	0.5785229028646306
1103	86-87	0.6128270524141541
1103	88-89	0.6686308955487483
1103	90-91	0.6406272763534808
1103	92-93	0.6462338667872451
1103	94-95	0.6080347326238815
1103	96-97	0.6088540464367895
1103	98-99	0.6269670260009192
1103	100	0.734674728635504
1104	1	0.4038584913658596
1104	2	0.5578129419968327
1104	3	0.612327913541094
1104	4	0.6760362342733117
1104	5	0.5927392343590938
1104	6	0.3278261608878381
1104	7	0.3361535194569214
1104	8	0.39671147571475274
1104	9	0.46898454798102307
1104	10-11	0.5097571088958972
1104	12-13	0.3913782146865614
1104	14-15	0.5662116624759364
1104	16-17	0.6847874235191966
1104	18-19	0.6498633178944075
1104	20-21	0.44887136994400834
1104	22-23	0.7228113023374974
1104	24-25	0.571999042780007
1104	26-27	0.4839071838960578
1104	28-29	0.6943750264140647
1104	30-31	0.7256554583633914
1104	32-33	0.5219219721014383
1104	34-35	0.40817395416191005
1104	36-37	0.4268941495570502
1104	38-39	0.5261677475498985
1104	40-41	0.44214156510203395
1104	42-43	0.38111477781006897
1104	44-45	0.47417354929064714
1104	46-47	0.44297189899211986
1104	48-49	0.4258848227844041
1104	50-51	0.5280702342661243
1104	52-53	0.47837023338520623
1104	54-55	0.5335992193233494
1104	56-57	0.34845113681056006
1104	58-59	0.43658419554009953
1104	60-61	0.43020790520355234
1104	62-63	0.34002354616905706
1104	64-65	0.3766687333876675
1104	66-67	0.4770058156386341
1104	68-69	0.44329137551238773
1104	70-71	0.3409431631979558
1104	72-73	0.2873637473357249
1104	74-75	0.29146701806377706
1104	76-77	0.32383505999235496
1104	78-79	0.2486213539056905
1104	80-81	0.31302512227185275
1104	82-83	0.29828022950498934
1104	84-85	0.33307621558169487
1104	86-87	0.3209300154723991
1104	88-89	0.2327395843836868
1104	90-91	0.27190702432682556
1104	92-93	0.26752947055997467
1104	94-95	0.24611868009954918
1104	96-97	0.2902407627963406
1104	98-99	0.43435344583261326
1104	100	0.4606957909208482
1105	1	0.29192598965909156
1105	2	0.4528908175159039
1105	3	0.3839583671618598
1105	4	0.49138512539146717
1105	5	0.39655812686155656
1105	6	0.10106201251178248
1105	7	0.1915324475226612
1105	8	0.16203796719798902
1105	9	0.10112331889688875
1105	10-11	0.2674738103761669
1105	12-13	0.20204243925407184
1105	14-15	0.3716889545508266
1105	16-17	0.5287017533631797
1105	18-19	0.47484532370268795
1105	20-21	0.3541715995357251
1105	22-23	0.5578281356722243
1105	24-25	0.46435771525374747
1105	26-27	0.2391720518581124
1105	28-29	0.5537505543859993
1105	30-31	0.5443261899067622
1105	32-33	0.4433776885863736
1105	34-35	0.3141656112516671
1105	36-37	0.17574950641247966
1105	38-39	0.3611697749621072
1105	40-41	0.21683757135431136
1105	42-43	0.30554207927718124
1105	44-45	0.33112749142655673
1105	46-47	0.304360169504136
1105	48-49	0.30072300222636983
1105	50-51	0.4262068044587366
1105	52-53	0.35130194592722574
1105	54-55	0.28662762089775384
1105	56-57	0.23771605273289964
1105	58-59	0.21758997729825325
1105	60-61	0.2154769297239767
1105	62-63	0.24073998023438037
1105	64-65	0.17036455324882382
1105	66-67	0.2862376807452307
1105	68-69	0.2558082479056836
1105	70-71	0.2009052055359959
1105	72-73	0.20685727033504264
1105	74-75	0.20308742364068877
1105	76-77	0.2183714293330823
1105	78-79	0.1718522369163793
1105	80-81	0.21883143282853013
1105	82-83	0.2192585685311883
1105	84-85	0.17307124515175687
1105	86-87	0.20572558655772255
1105	88-89	0.18414695544408843
1105	90-91	0.14812814959873677
1105	92-93	0.2610049183437546
1105	94-95	0.17283072616177364
1105	96-97	0.2143871150712009
1105	98-99	0.2606445041090879
1105	100	0.26430721884034725
1106	1	0.45681098534516096
1106	2	0.4582950970703159
1106	3	0.46120810436424264
1106	4	0.5617182285444784
1106	5	0.4039415486081879
1106	6	0.16960800464268289
1106	7	0.20259634427628015
1106	8	0.2892592371451528
1106	9	0.30936298940573437
1106	10-11	0.29582689358511516
1106	12-13	0.261479919680248
1106	14-15	0.3415848551099927
1106	16-17	0.5546713868219086
1106	18-19	0.48968752439550656
1106	20-21	0.3532664753287946
1106	22-23	0.6171651130529483
1106	24-25	0.41189599776699026
1106	26-27	0.3236976494799606
1106	28-29	0.5553765759087383
1106	30-31	0.5263659617059702
1106	32-33	0.41068569024014323
1106	34-35	0.31984672464866293
1106	36-37	0.2489678285457515
1106	38-39	0.3646683758385194
1106	40-41	0.3292045898837799
1106	42-43	0.3067613142356507
1106	44-45	0.3355262584973815
1106	46-47	0.28407835194081343
1106	48-49	0.35599996415094637
1106	50-51	0.3229012883300868
1106	52-53	0.28818584974875705
1106	54-55	0.3640526789317491
1106	56-57	0.31012101979838747
1106	58-59	0.3059831460384288
1106	60-61	0.22325978135425828
1106	62-63	0.19928534894111039
1106	64-65	0.288036530405968
1106	66-67	0.3164988642540365
1106	68-69	0.2653350574861406
1106	70-71	0.17649026478562035
1106	72-73	0.15144900141685014
1106	74-75	0.22263546682791713
1106	76-77	0.26050382782397463
1106	78-79	0.27104143201150066
1106	80-81	0.32615725440563637
1106	82-83	0.28730117319184956
1106	84-85	0.27695249900741814
1106	86-87	0.3169000653535434
1106	88-89	0.3860313835295379
1106	90-91	0.24937304915812675
1106	92-93	0.31667107277427675
1106	94-95	0.3763538118157541
1106	96-97	0.40283379132519315
1106	98-99	0.42220294883932397
1106	100	0.40256874316660785
1107	1	0.43418338696443826
1107	2	0.551754108466497
1107	3	0.4465335280337932
1107	4	0.5601786264752562
1107	5	0.4594793966320907
1107	6	0.2639577552524415
1107	7	0.2737027393718705
1107	8	0.26394098514216324
1107	9	0.425333659409759
1107	10-11	0.43536290944908274
1107	12-13	0.29262599933039013
1107	14-15	0.5042880414799598
1107	16-17	0.5880042807604724
1107	18-19	0.7523051868748567
1107	20-21	0.4566742444606504
1107	22-23	0.6549548415934581
1107	24-25	0.3997355964512508
1107	26-27	0.3873641147224802
1107	28-29	0.49169031465325475
1107	30-31	0.5352230094306591
1107	32-33	0.4882159903453811
1107	34-35	0.3660291489881331
1107	36-37	0.33290853796418673
1107	38-39	0.31094525145437046
1107	40-41	0.15199518047498373
1107	42-43	0.33716626975309083
1107	44-45	0.20991499992942408
1107	46-47	0.26042057044081446
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2216	100	-0.5036877567698106
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16999.0
1	2832.0
2	459.0
3	408.0
4	400.0
5	223.0
6	205.0
7	266.0
8	562.0
9	998.0
10	1627.0
11	2771.0
12	4285.0
13	6033.0
14	8303.0
15	11055.0
16	13874.0
17	16360.0
18	18811.0
19	21123.0
20	23969.0
21	27178.0
22	31396.0
23	36226.0
24	41397.0
25	47363.0
26	53925.0
27	62091.0
28	73786.0
29	88644.0
30	105116.0
31	127755.0
32	157292.0
33	201769.0
34	279991.0
35	377943.0
36	559868.0
37	835913.0
38	666019.0
39	223499.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.90834238887006	21.668657911033733	11.987364118263361	24.43563558183284
2	15.359172978969534	21.056515589447145	40.770560158671515	22.81375127291181
3	17.968389735601047	28.954891748722183	29.293770368561983	23.782948147114784
4	11.923811128974334	17.731546494307942	41.35616500388543	28.98847737283229
5	12.665288911084897	39.22018420803545	35.47107354789169	12.643453332987967
6	27.71552934126834	39.64764271235522	19.560942445177147	13.075885501199286
7	25.142507063277712	32.39652900734271	25.61335994482613	16.847603984553448
8	25.16405372589946	34.90279356459773	21.41838642065391	18.514766288848904
9	26.704096402122605	14.858488851760123	22.357172035127387	36.080242710989886
10-11	25.209975907730907	25.985762722953442	28.063162090629824	20.741099278685823
12-13	25.75882440886898	24.21557511890953	27.719919929767716	22.305680542453775
14-15	23.170080729362912	25.04929126466956	25.504476739635752	26.27615126633178
16-17	22.135082123249756	27.243839711273743	26.892345926295953	23.72873223918055
18-19	21.530129828558746	28.15958019470225	28.310408953235815	21.99988102350319
20-21	23.345719457320573	27.23324602064973	26.876431148577755	22.544603373451935
22-23	23.87884361913595	26.953193929100987	26.519386999889427	22.64857545187364
24-25	23.229882563404168	27.113663063959592	27.314715368937343	22.3417390036989
26-27	23.00401202274408	27.549557965261762	26.397103765816677	23.049326246177483
28-29	22.262654973256645	28.100743125429585	26.818777260308558	22.81782464100522
30-31	22.97974371156685	27.663133661654786	26.8060445906307	22.551078036147665
32-33	23.41456252425131	27.904763208491058	25.8259013128143	22.854772954443337
34-35	23.357863478474737	27.46970785542157	26.93162777342288	22.240800892680817
36-37	23.15827944631658	27.32534953601403	26.597964178943144	22.918406838726234
38-39	23.388900372630587	27.14152329019943	27.143461928892187	22.326114408277796
40-41	22.82851170821847	26.395649934870196	27.78962164127109	22.986216715640243
42-43	22.76836383415524	26.796633978141383	27.06362951319086	23.37137267451251
44-45	22.71475643138377	27.844061505903127	26.944744467230425	22.49643759548268
46-47	22.693108635971733	27.26265513127576	26.58200728646672	23.462228946285787
48-49	23.50304335885346	26.4904935241021	27.106736893955574	22.89972622308887
50-51	23.17587892694477	26.68225713957189	26.56808505559755	23.57377887788579
52-53	22.444053304075346	27.346422212551406	26.60236435306923	23.60716013030402
54-55	22.571685131757715	27.319186719335097	26.457018000782973	23.652110148124212
56-57	23.630593087904142	26.515206492161088	26.348405327315184	23.505795092619593
58-59	22.48279819362102	26.748552971604404	27.567609874868527	23.201038959906047
60-61	22.674938570116325	27.549652137609726	26.507446094232144	23.2679631980418
62-63	23.264227589090975	26.760413229187712	26.012102833379124	23.963256348342185
64-65	22.014034100608534	26.941525873139167	26.76083231470581	24.28360771154649
66-67	23.17240306455998	26.318149458603774	26.561357350587194	23.948090126249056
68-69	22.207590483442775	26.608995776655707	26.79549086622425	24.38792287367727
70-71	22.279127304625547	26.282599766644736	26.96601809411996	24.47225483460976
72-73	22.985688619765302	26.425649304544173	27.333371944189054	23.255290131501464
74-75	23.026848821127203	26.899369777553222	26.814156772301473	23.2596246290181
76-77	22.54236676242897	26.78691753046169	26.729191203888902	23.94152450322044
78-79	22.471968525349205	26.86524460692678	26.577786115176806	24.08500075254721
80-81	22.109210705175876	27.000726675685293	27.446763555142667	23.443299063996164
82-83	23.028456291698586	25.84924909986362	28.263379802367982	22.858914806069812
84-85	23.30406476798415	26.485868685599534	26.766809594713024	23.443256951703294
86-87	21.841073980279475	27.11987605825787	27.406493767547495	23.632556193915157
88-89	22.91729800818377	25.702499570169902	27.253738425202435	24.126463996443896
90-91	22.62998503080467	25.80753362586174	27.370441407284684	24.192039936048907
92-93	22.903370286011437	26.0139161642969	27.347365814942894	23.735347734748764
94-95	21.723917768961584	26.102542670266793	27.892086383530394	24.281453177241225
96-97	22.172663050305097	25.994807069510482	27.34229833289411	24.490231547290307
98-99	21.603363087061485	26.78841876793615	27.20104386791562	24.407174277086746
100	22.400192001715347	25.856027952330944	26.511221623096738	25.232558422856975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14222.0
1	8394.5
2	2294.0
3	1622.5
4	913.0
5	523.0
6	401.0
7	358.0
8	365.0
9	361.5
10	319.0
11	310.5
12	327.0
13	329.0
14	340.5
15	377.0
16	398.0
17	422.5
18	518.5
19	639.0
20	772.5
21	995.0
22	1376.5
23	1914.5
24	2634.5
25	3564.0
26	5175.0
27	7318.0
28	10524.0
29	17847.5
30	28605.5
31	39693.5
32	54108.5
33	69171.5
34	85857.5
35	101251.0
36	115445.5
37	140200.5
38	148123.5
39	136952.5
40	126036.5
41	114938.5
42	113035.5
43	120228.5
44	132539.5
45	156789.0
46	178469.5
47	192184.0
48	199977.5
49	192960.0
50	182192.0
51	182161.0
52	182356.5
53	175384.5
54	165178.5
55	143757.0
56	127139.5
57	105179.5
58	81126.5
59	73694.0
60	60009.0
61	42307.5
62	31226.0
63	24896.0
64	18059.5
65	9374.0
66	5168.0
67	3450.0
68	2514.0
69	1863.5
70	1445.5
71	1187.5
72	934.0
73	796.5
74	672.5
75	517.0
76	374.5
77	234.0
78	140.0
79	105.0
80	83.5
81	59.5
82	38.0
83	32.5
84	28.0
85	16.0
86	15.5
87	14.0
88	11.5
89	10.5
90	10.0
91	10.5
92	9.5
93	7.5
94	4.0
95	3.5
96	6.0
97	5.5
98	3.0
99	1.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9847094535867634
2	0.4469685246808865
3	0.48027905644905877
4	0.46545562878087793
5	0.4330128098518187
6	0.485220199005119
7	0.5012728865287099
8	0.5042857783311858
9	0.5172532646490414
10-11	0.5301604931308477
12-13	0.5293289349933644
14-15	0.526388352594148
16-17	0.5308112777601826
18-19	0.5296061210391922
20-21	0.5280514688691147
22-23	0.49142675611821907
24-25	0.5255085881878252
26-27	0.5060453071438316
28-29	0.5126977722436982
30-31	0.5153009107610372
32-33	0.5178196883079069
34-35	0.5288950785738079
36-37	0.5468880684181933
38-39	0.5358367812867121
40-41	0.5414287084721071
42-43	0.5390545497317563
44-45	0.5486475972308391
46-47	0.5470326872247121
48-49	0.5199648672712697
50-51	0.5344267479231535
52-53	0.5272319622988413
54-55	0.5367165456930351
56-57	0.5246890816175518
58-59	0.5307148652225033
60-61	0.5248457519912805
62-63	0.519181515402626
64-65	0.5293409865605743
66-67	0.5282442939444731
68-69	0.5477196265556766
70-71	0.5322695173925808
72-73	0.5348967590443396
74-75	0.5331733849333234
76-77	0.5207482191399134
78-79	0.5507807246269919
80-81	0.5260509087122708
82-83	0.5260388571450608
84-85	0.534354438519894
86-87	0.8351012982430261
88-89	1.0960297799046383
90-91	1.086496990241605
92-93	1.0875575281560765
94-95	1.091341720259986
96-97	1.084387965979872
98-99	1.081519692983915
100	1.0776029336406965
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4148838.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.61753670480433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.04846936404157	19.976053879367594
2	12.394114530932741	6.59801596901302
3	4.736141945783362	3.7819329624315614
4	2.2993851358392865	2.4481587300673477
5	1.305614863810998	1.7376125779913671
6	0.8386505925560948	1.3393687757920654
7	0.5373936825740719	1.0012867249591728
8	0.3740807255097513	0.7965685953452512
9	0.2938732264282206	0.7039963251911193
>10	1.5869082352324728	8.057396520382696
>50	0.21203756761486175	4.006692396784232
>100	0.2739861574477661	16.445635618645564
>500	0.05822987615600436	11.018332568802446
>1k	0.039484021779185925	18.644643752701285
>5k	0.001177275878737195	2.0364662782982133
>10k+	4.527984148989211E-4	1.4078383242271573
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12699	0.3060857039971192	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12451	0.3001081266610073	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11785	0.2840554391374163	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	11245	0.27103974655072094	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10225	0.2464545494425186	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	9784	0.23582506716338406	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	8981	0.21647025022427965	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7738	0.18651005414046054	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7554	0.1820750774072162	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6537	0.15756218970227326	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6068	0.14625781965938414	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6017	0.14502855980397403	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5617	0.13538730603605154	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5460	0.13160311393214197	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5420	0.13063898855534972	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5184	0.12495064883227544	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5072	0.12225109777725715	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5052	0.12176903508886103	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4816	0.11608069536578676	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4801	0.11571914834948965	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4757	0.1146586104350182	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4731	0.11403192894010324	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4618	0.11130827475066513	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4547	0.10959695220685889	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4400	0.10605379144714737	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	4364	0.10518607860803436	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4252	0.10248652755301606	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	4202	0.10128137083202575	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	4192	0.10104033948782767	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	4178	0.10070289560595037	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.205156720990311E-5	2.410313441980622E-5
14-15	0.0	0.0	0.0	2.410313441980622E-5	6.025783604951555E-5
16-17	0.0	0.0	0.0	2.410313441980622E-5	1.0846410488912799E-4
18-19	0.0	0.0	0.0	2.410313441980622E-5	1.205156720990311E-4
20-21	0.0	0.0	0.0	2.410313441980622E-5	1.4461880651883731E-4
22-23	0.0	0.0	0.0	8.436097046932178E-5	1.4461880651883731E-4
24-25	0.0	0.0	0.0	2.651344786178684E-4	1.4461880651883731E-4
26-27	0.0	0.0	0.0	7.59248734223896E-4	1.4461880651883731E-4
28-29	0.0	0.0	0.0	0.002398261874770719	1.4461880651883731E-4
30-31	0.0	0.0	0.0	0.010014852351429484	1.6872194093864356E-4
32-33	0.0	0.0	0.0	0.03029763996569642	2.1692820977825599E-4
34-35	0.0	0.0	0.0	0.060776053439541386	2.651344786178684E-4
36-37	0.0	0.0	0.0	0.11065749012133036	2.651344786178684E-4
38-39	0.0	0.0	0.0	0.1959705344002345	2.651344786178684E-4
40-41	0.0	0.0	0.0	0.3012289224115282	2.651344786178684E-4
42-43	0.0	0.0	0.0	0.41054868857255933	2.651344786178684E-4
44-45	0.0	0.0	0.0	0.535101635686908	2.651344786178684E-4
46-47	0.0	0.0	0.0	0.6884939831345547	2.651344786178684E-4
48-49	0.0	0.0	0.0	0.870942659125278	2.651344786178684E-4
50-51	0.0	0.0	0.0	1.0727943583239452	2.771860458277715E-4
52-53	0.0	0.0	0.0	1.2766948239482958	3.374438818772871E-4
54-55	0.0	0.0	0.0	1.4908391217010641	3.374438818772871E-4
56-57	0.0	0.0	0.0	1.7482485457373849	3.374438818772871E-4
58-59	0.0	0.0	0.0	2.0588776905726376	3.374438818772871E-4
60-61	0.0	0.0	0.0	2.3986957311902755	3.494954490871902E-4
62-63	0.0	0.0	0.0	2.7641595068305875	3.615470162970933E-4
64-65	0.0	0.0	0.0	3.139855063032107	3.615470162970933E-4
66-67	0.0	0.0	0.0	3.5466316110679665	3.615470162970933E-4
68-69	0.0	0.0	0.0	3.9984207626328145	3.615470162970933E-4
70-71	0.0	0.0	0.0	4.509322851362237	3.615470162970933E-4
72-73	0.0	0.0	0.0	5.060935134126712	3.977017179268026E-4
74-75	0.0	0.0	0.0	5.598483237957231	4.3385641955651197E-4
76-77	0.0	0.0	0.0	6.19310756409385	4.3385641955651197E-4
78-79	0.0	0.0	0.0	6.818186200569894	4.3385641955651197E-4
80-81	0.0	2.410313441980622E-5	0.0	7.5100907772248515	4.4590798676641505E-4
82-83	0.0	2.410313441980622E-5	0.0	8.263578862322415	4.820626883961244E-4
84-85	0.0	2.410313441980622E-5	0.0	8.997302377195735	4.820626883961244E-4
86-87	0.0	2.410313441980622E-5	0.0	9.760708902107048	5.664236588654462E-4
88	0.0	2.410313441980622E-5	0.0	10.35246495524771	6.50784629334768E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9090	0.0	36.063526	1
CAACGCA	8840	0.0	33.848213	5
TATTCTC	2460	0.0	33.415775	5
GGGCACC	3600	0.0	32.129757	94
ATCAACG	9415	0.0	31.932623	3
TATCAAC	10860	0.0	29.977411	2
AACGCAG	11375	0.0	27.692144	6
ACGCAGA	10725	0.0	27.534737	7
CGCAGAG	10740	0.0	27.408854	8
GTACATG	19290	0.0	27.04395	1
TACATGG	19190	0.0	26.732424	2
GTACTAG	3995	0.0	26.565193	1
TACCCCG	375	0.0	26.304897	5
ACATGGG	18690	0.0	26.189869	3
GGTATCA	5635	0.0	25.362726	1
CATGGGG	14870	0.0	24.60942	4
CTGTGCG	5040	0.0	24.594488	9
ATGGGGG	13000	0.0	24.35371	5
TATTCGC	540	0.0	24.34606	9
GCAGAGT	11585	0.0	24.317541	9
>>END_MODULE