##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146417_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4148838 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.802901197877574 31.0 31.0 34.0 30.0 34.0 2 31.797378928750653 31.0 31.0 34.0 30.0 34.0 3 31.97437402954755 31.0 31.0 34.0 30.0 34.0 4 35.71212807055855 37.0 35.0 37.0 35.0 37.0 5 35.632250765154005 37.0 35.0 37.0 33.0 37.0 6 35.656599510513544 37.0 35.0 37.0 33.0 37.0 7 35.582198919311864 37.0 35.0 37.0 33.0 37.0 8 35.561447325733134 37.0 35.0 37.0 33.0 37.0 9 37.15090490397552 39.0 37.0 39.0 33.0 39.0 10-11 37.093030747404455 39.0 37.0 39.0 33.0 39.0 12-13 37.02954996073599 39.0 37.0 39.0 33.0 39.0 14-15 38.20432263202372 40.0 37.0 41.0 33.0 41.0 16-17 38.20830073384403 40.0 37.0 41.0 33.0 41.0 18-19 38.25481038305183 40.0 37.5 41.0 33.0 41.0 20-21 38.26495647697018 40.0 37.5 41.0 33.0 41.0 22-23 38.23132236062242 40.0 37.0 41.0 33.0 41.0 24-25 38.15924241920268 40.0 37.0 41.0 33.0 41.0 26-27 38.010273478983756 40.0 37.0 41.0 33.0 41.0 28-29 37.946343891952395 40.0 37.0 41.0 33.0 41.0 30-31 37.81369723281555 40.0 37.0 41.0 32.5 41.0 32-33 37.69276349185 40.0 36.0 41.0 32.0 41.0 34-35 37.5937260023168 40.0 36.0 41.0 32.0 41.0 36-37 37.50438484221365 40.0 36.0 41.0 32.0 41.0 38-39 37.38499177360022 40.0 36.0 41.0 31.0 41.0 40-41 37.255059729977404 39.5 36.0 41.0 31.0 41.0 42-43 37.130159095149054 39.0 35.0 41.0 31.0 41.0 44-45 36.92587900515759 39.0 35.0 41.0 30.5 41.0 46-47 36.69353262286934 39.0 35.0 41.0 30.0 41.0 48-49 36.43960525814698 39.0 35.0 41.0 30.0 41.0 50-51 36.24022774569651 39.0 35.0 41.0 29.0 41.0 52-53 36.040150157706805 38.0 35.0 40.5 29.0 41.0 54-55 35.831190805714755 38.0 34.5 40.0 29.0 41.0 56-57 35.5627748058613 37.5 34.0 40.0 28.0 41.0 58-59 35.28356771221243 37.0 34.0 40.0 28.0 41.0 60-61 35.01397861280677 36.5 34.0 40.0 28.0 41.0 62-63 34.73364542071781 36.0 34.0 39.0 27.0 41.0 64-65 34.470391589163036 35.5 33.0 39.0 27.0 41.0 66-67 34.24397048040922 35.0 33.0 39.0 26.5 41.0 68-69 34.05422096500273 35.0 33.0 38.5 26.0 41.0 70-71 33.86043706695706 35.0 33.0 37.5 26.0 40.0 72-73 33.664766496064686 35.0 33.0 37.0 26.0 40.0 74-75 33.47700018655826 35.0 33.0 37.0 26.0 39.5 76-77 33.33215240026244 35.0 33.0 36.0 26.0 39.0 78-79 33.22034061103374 35.0 33.0 36.0 27.0 39.0 80-81 33.0774802486865 35.0 33.0 36.0 27.0 38.0 82-83 32.890887882342 35.0 33.0 35.0 26.5 37.0 84-85 32.7370543270188 35.0 33.0 35.0 26.0 37.0 86-87 32.604683407739714 35.0 33.0 35.0 26.0 36.5 88-89 32.4971634226258 35.0 33.0 35.0 26.0 36.0 90-91 32.39986087670813 35.0 33.0 35.0 26.0 36.0 92-93 32.32120861311047 35.0 33.0 35.0 26.0 36.0 94-95 32.28103435226924 35.0 33.0 35.0 26.0 35.0 96-97 32.22828126815267 35.0 33.0 35.0 25.5 35.0 98-99 32.183687456584224 35.0 33.0 35.0 25.0 35.0 100 31.664295159271102 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5146199207384932 1101 2 0.6140924707748994 1101 3 0.5254245365659003 1101 4 0.39730421871795585 1101 5 0.4180921693761306 1101 6 0.4134595486654007 1101 7 0.44228928125056655 1101 8 0.40900789033096885 1101 9 0.5195495504579526 1101 10-11 0.5779794149342763 1101 12-13 0.5676119875091459 1101 14-15 0.6845183364001031 1101 16-17 0.6336097749336389 1101 18-19 0.6606226587440034 1101 20-21 0.6603197503232465 1101 22-23 0.6728835603664507 1101 24-25 0.7176649969576729 1101 26-27 0.7005559259585539 1101 28-29 0.7472890883571495 1101 30-31 0.8120664497020869 1101 32-33 0.8072489741196165 1101 34-35 0.7593782210561528 1101 36-37 0.7946616539324012 1101 38-39 0.8096642432928931 1101 40-41 0.835018407733628 1101 42-43 0.7926769042032333 1101 44-45 0.8533252025016509 1101 46-47 0.8281061314719409 1101 48-49 0.759266349462159 1101 50-51 0.7749515671540337 1101 52-53 0.8634778038928914 1101 54-55 0.8064956052875374 1101 56-57 0.7014343266625431 1101 58-59 0.7782383183911605 1101 60-61 0.7955068497590645 1101 62-63 0.6876466475833709 1101 64-65 0.725982343596641 1101 66-67 0.6716747320339636 1101 68-69 0.6733335968320091 1101 70-71 0.656564213490789 1101 72-73 0.6017589336954643 1101 74-75 0.6248844791218247 1101 76-77 0.5957605613163679 1101 78-79 0.6111773671770493 1101 80-81 0.611365797830004 1101 82-83 0.6012164266009492 1101 84-85 0.5949096051010727 1101 86-87 0.5540692171697259 1101 88-89 0.6140169171489802 1101 90-91 0.6238172436820406 1101 92-93 0.6313951725342548 1101 94-95 0.5981155401929925 1101 96-97 0.6219124299288481 1101 98-99 0.6650927954819323 1101 100 0.7449682958736652 1102 1 0.40209412146548473 1102 2 0.5482818480672051 1102 3 0.4425113820271136 1102 4 0.3234586097663623 1102 5 0.29387144337130877 1102 6 0.32377717877707113 1102 7 0.35774541112252933 1102 8 0.3418264142322869 1102 9 0.39754980161639963 1102 10-11 0.43162797434543165 1102 12-13 0.46697045224748734 1102 14-15 0.5983176516819668 1102 16-17 0.5909247058030402 1102 18-19 0.6076139698484582 1102 20-21 0.5698955509667911 1102 22-23 0.5606367592899701 1102 24-25 0.6241484766701504 1102 26-27 0.6495362530735562 1102 28-29 0.6084571634218463 1102 30-31 0.6268274978418873 1102 32-33 0.6362530769462964 1102 34-35 0.6791828563661966 1102 36-37 0.6350248166870429 1102 38-39 0.6734405389863056 1102 40-41 0.6422885032410122 1102 42-43 0.7085279270364424 1102 44-45 0.6980638032941329 1102 46-47 0.6619691719572387 1102 48-49 0.6095089586149456 1102 50-51 0.6321873600666876 1102 52-53 0.5739799633798768 1102 54-55 0.6162340852081769 1102 56-57 0.6485495497917171 1102 58-59 0.6677660654905893 1102 60-61 0.6928551397853866 1102 62-63 0.5706770551627258 1102 64-65 0.5870249129916587 1102 66-67 0.5632126661042349 1102 68-69 0.5492354221242834 1102 70-71 0.5279504580595855 1102 72-73 0.5514503451897426 1102 74-75 0.5292760301360389 1102 76-77 0.4600720397623732 1102 78-79 0.47054239233874284 1102 80-81 0.44871933756797944 1102 82-83 0.4871723004844952 1102 84-85 0.4989811154317465 1102 86-87 0.4763534169253276 1102 88-89 0.4356985156150799 1102 90-91 0.4302726188527899 1102 92-93 0.4749728397528372 1102 94-95 0.4583539686021183 1102 96-97 0.45772947527088803 1102 98-99 0.492435658289061 1102 100 0.5585440521893652 1103 1 0.28859106509774435 1103 2 -0.09360552455083848 1103 3 0.24598102241206732 1103 4 0.24776522836140913 1103 5 0.28874290788048995 1103 6 0.2775975694634525 1103 7 0.338366672259248 1103 8 0.3715463421077345 1103 9 0.4164328245519755 1103 10-11 0.4559683448244556 1103 12-13 0.46367288747399016 1103 14-15 0.6321702822212103 1103 16-17 0.6008144740721377 1103 18-19 0.6032669279722285 1103 20-21 0.556039484339216 1103 22-23 0.5494051942464111 1103 24-25 0.5723839630032117 1103 26-27 0.5821225028395887 1103 28-29 0.5787754407378713 1103 30-31 0.6476038425575723 1103 32-33 0.6534850932202829 1103 34-35 0.6378973415957319 1103 36-37 0.746327625134974 1103 38-39 0.7133909654904116 1103 40-41 0.6863383039697766 1103 42-43 0.650373987614941 1103 44-45 0.6907950871988646 1103 46-47 0.6738289578578218 1103 48-49 0.7362201396425618 1103 50-51 0.7434463015326216 1103 52-53 0.7683671925500306 1103 54-55 0.6438810840833327 1103 56-57 0.6671613301412762 1103 58-59 0.6942050571285563 1103 60-61 0.6796985183879869 1103 62-63 0.6233314928693758 1103 64-65 0.6802178659560738 1103 66-67 0.7313303291492872 1103 68-69 0.6632201350995786 1103 70-71 0.5633763764805124 1103 72-73 0.5416577313427879 1103 74-75 0.5209773439473935 1103 76-77 0.5377594419359113 1103 78-79 0.49123859179287166 1103 80-81 0.529292325993282 1103 82-83 0.46599342149427514 1103 84-85 0.4882211680226405 1103 86-87 0.5157825542882577 1103 88-89 0.5355288950321224 1103 90-91 0.48929509597083154 1103 92-93 0.5412233775039965 1103 94-95 0.50727995073008 1103 96-97 0.465667376942811 1103 98-99 0.5255456902872098 1103 100 0.606112988770942 1104 1 0.34287153457326625 1104 2 0.41200364109575105 1104 3 0.34466672142050925 1104 4 0.32057322969578905 1104 5 0.305887759351414 1104 6 0.28645867864276653 1104 7 0.3275727648056659 1104 8 0.31864304817138134 1104 9 0.3297043910355981 1104 10-11 0.41259568729816465 1104 12-13 0.3673114273605691 1104 14-15 0.48789466435239603 1104 16-17 0.47303484656323747 1104 18-19 0.5350036150918598 1104 20-21 0.5966656122597911 1104 22-23 0.5031943743193779 1104 24-25 0.5003395071290626 1104 26-27 0.58901526159638 1104 28-29 0.5643112900562102 1104 30-31 0.5620800305085254 1104 32-33 0.5727160942898735 1104 34-35 0.5480708250695301 1104 36-37 0.6294711543412248 1104 38-39 0.5878512838798571 1104 40-41 0.615020832185337 1104 42-43 0.5776702572908761 1104 44-45 0.5858332183687622 1104 46-47 0.5316145159237919 1104 48-49 0.5524524156351305 1104 50-51 0.5865507720019565 1104 52-53 0.5556584601533956 1104 54-55 0.5055248530257472 1104 56-57 0.533401372557968 1104 58-59 0.5510531394502678 1104 60-61 0.527333295893925 1104 62-63 0.5167826449516539 1104 64-65 0.46065839514820084 1104 66-67 0.43338834897078016 1104 68-69 0.4796134279184514 1104 70-71 0.45868094233052403 1104 72-73 0.4765498436468434 1104 74-75 0.4146077706043627 1104 76-77 0.43845215375469593 1104 78-79 0.41617814396607855 1104 80-81 0.3886008463431452 1104 82-83 0.3729172551223314 1104 84-85 0.34831750104103065 1104 86-87 0.3602883069469627 1104 88-89 0.3811010515804796 1104 90-91 0.31482983239224893 1104 92-93 0.3921587476436059 1104 94-95 0.4238823865155652 1104 96-97 0.4794379625541225 1104 98-99 0.3218390829456865 1104 100 0.4168228463586665 1105 1 0.17511498245296409 1105 2 -0.37584193186075154 1105 3 0.041251040513394344 1105 4 0.10579288600218462 1105 5 0.1512296344606412 1105 6 0.21479238197277795 1105 7 0.24812282208551295 1105 8 0.2460545151131157 1105 9 0.30864773503767395 1105 10-11 0.3390933654025119 1105 12-13 0.32221667490650674 1105 14-15 0.3975476989125113 1105 16-17 0.34879444488854716 1105 18-19 0.4190379074250785 1105 20-21 0.3732869334422233 1105 22-23 0.32440146030672423 1105 24-25 0.34679300726731555 1105 26-27 0.31732295010301925 1105 28-29 0.3563127401282884 1105 30-31 0.4258698579859228 1105 32-33 0.39543806231852585 1105 34-35 0.39762457638163085 1105 36-37 0.4167704069237246 1105 38-39 0.4639343870704167 1105 40-41 0.4530652041726029 1105 42-43 0.44715468781875956 1105 44-45 0.4647801775592768 1105 46-47 0.43372120794643365 1105 48-49 0.46521560226205594 1105 50-51 0.4429320065215947 1105 52-53 0.33772440954729177 1105 54-55 0.3745584750049318 1105 56-57 0.43172946598751594 1105 58-59 0.3728472393900617 1105 60-61 0.33176263748999446 1105 62-63 0.3205011205992676 1105 64-65 0.38355936950507186 1105 66-67 0.38635492900614565 1105 68-69 0.27343755063574804 1105 70-71 0.3791829939045428 1105 72-73 0.3761881249680954 1105 74-75 0.3316797531846092 1105 76-77 0.37816738772278313 1105 78-79 0.3127178545402671 1105 80-81 0.3610762276365307 1105 82-83 0.29442588234262956 1105 84-85 0.22904848306065162 1105 86-87 0.25861258535353926 1105 88-89 0.26909597360322124 1105 90-91 0.22038103599566483 1105 92-93 0.2340354010473078 1105 94-95 0.272310115628585 1105 96-97 0.26284979755474325 1105 98-99 0.28602936975185145 1105 100 0.29401044411863 1106 1 0.23734304350005786 1106 2 0.3243755366680361 1106 3 0.2658615863345659 1106 4 0.22076100185000058 1106 5 0.2055125722593445 1106 6 0.21884020898147583 1106 7 0.20950272786674873 1106 8 0.24388368605721666 1106 9 0.2904528161007178 1106 10-11 0.2628513671985928 1106 12-13 0.2781773811882431 1106 14-15 0.3238742610697827 1106 16-17 0.26685005515057014 1106 18-19 0.33052059326493577 1106 20-21 0.2600867507486413 1106 22-23 0.3058501268354874 1106 24-25 0.2522303838506801 1106 26-27 0.32116628204902753 1106 28-29 0.32127042578679976 1106 30-31 0.31307488952393214 1106 32-33 0.3178485061515488 1106 34-35 0.24328990998562716 1106 36-37 0.300797800034708 1106 38-39 0.28439106179936147 1106 40-41 0.30293564396025374 1106 42-43 0.26838358447459143 1106 44-45 0.3074965725406358 1106 46-47 0.28903677350738377 1106 48-49 0.20730847896611238 1106 50-51 0.21828506751129595 1106 52-53 0.2850186361896192 1106 54-55 0.21927612812786634 1106 56-57 0.2764355733683317 1106 58-59 0.3052030890781836 1106 60-61 0.26468966858343634 1106 62-63 0.2679214896388302 1106 64-65 0.2646731498326105 1106 66-67 0.2487755101384721 1106 68-69 0.23928943451065265 1106 70-71 0.23722307916032292 1106 72-73 0.24172536785956567 1106 74-75 0.1926350235565053 1106 76-77 0.17866982949804822 1106 78-79 0.1762318610633642 1106 80-81 0.21073062661936603 1106 82-83 0.2132004388507056 1106 84-85 0.20490820702239176 1106 86-87 0.19346594203359757 1106 88-89 0.17008395859440384 1106 90-91 0.1669952230179561 1106 92-93 0.18991139647161503 1106 94-95 0.14634937445713092 1106 96-97 0.2252323925068751 1106 98-99 0.24964391604641634 1106 100 0.35466474935830306 1107 1 0.09045479074939777 1107 2 -0.5304249743876 1107 3 -0.054782328331530294 1107 4 0.02484038472214678 1107 5 0.05134729119734516 1107 6 0.1757807538942231 1107 7 0.2201516344293779 1107 8 0.14455533699494794 1107 9 0.18812095969176568 1107 10-11 0.22424243477257733 1107 12-13 0.16211580993859087 1107 14-15 0.2753720415914884 1107 16-17 0.2919286975146633 1107 18-19 0.2282142752238343 1107 20-21 0.2271726767435851 1107 22-23 0.20293895516073945 1107 24-25 0.21195873745103455 1107 26-27 0.32459686251137754 1107 28-29 0.2018090084601596 1107 30-31 0.26060059946497915 1107 32-33 0.3005595425763943 1107 34-35 0.31686883058657145 1107 36-37 0.3057007598164674 1107 38-39 0.3639618507607949 1107 40-41 0.28903337949165575 1107 42-43 0.2965566470551906 1107 44-45 0.32679864998050334 1107 46-47 0.4456227245176123 1107 48-49 0.4249447759683278 1107 50-51 0.4110797512158797 1107 52-53 0.37082608754373325 1107 54-55 0.3637106400807113 1107 56-57 0.3132747329367973 1107 58-59 0.28773110247075095 1107 60-61 0.2924785397229428 1107 62-63 0.21301779985730462 1107 64-65 0.26399700497908185 1107 66-67 0.3004636206647149 1107 68-69 0.3130928416660197 1107 70-71 0.3703057110429242 1107 72-73 0.32736980142995975 1107 74-75 0.3164387422404502 1107 76-77 0.29176489323027965 1107 78-79 0.2316629408083486 1107 80-81 0.21067720179520677 1107 82-83 0.21521476163224662 1107 84-85 0.26207910406098733 1107 86-87 0.15656477898122745 1107 88-89 0.2058549704057029 1107 90-91 0.21859031669413298 1107 92-93 0.23078808249211136 1107 94-95 0.16776895107958012 1107 96-97 0.17250951892647493 1107 98-99 0.20330800576738994 1107 100 0.18825411732543174 1108 1 0.1492782839641471 1108 2 0.27553149261417786 1108 3 0.1569093306318763 1108 4 0.11957967263084868 1108 5 0.09367222205032277 1108 6 0.12521014279314358 1108 7 0.09014964252099844 1108 8 0.14833287501441816 1108 9 0.11667656174037688 1108 10-11 0.15497615673317 1108 12-13 0.09716945472070648 1108 14-15 0.21851081743843537 1108 16-17 0.18837217244214344 1108 18-19 0.17867310926915536 1108 20-21 0.17112298432706297 1108 22-23 0.21203950368764168 1108 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-0.8500388982083322 2215 28-29 -0.8395543133126964 2215 30-31 -0.9214107959734719 2215 32-33 -0.9093617269159822 2215 34-35 -0.8063683952637319 2215 36-37 -0.780463363761065 2215 38-39 -0.8480777000623689 2215 40-41 -0.8546947192079131 2215 42-43 -0.7961701121344902 2215 44-45 -0.8877337877589468 2215 46-47 -0.8523100073495158 2215 48-49 -0.8086103830656413 2215 50-51 -0.8401157672473616 2215 52-53 -0.8726276066536514 2215 54-55 -0.8864843008487497 2215 56-57 -0.8133271074486643 2215 58-59 -0.7531147615719433 2215 60-61 -0.7567688949411604 2215 62-63 -0.7855738061354813 2215 64-65 -0.7885752426064059 2215 66-67 -0.7324682884642186 2215 68-69 -0.7574168135268025 2215 70-71 -0.6789337730987626 2215 72-73 -0.6415595324647327 2215 74-75 -0.6225273512437752 2215 76-77 -0.635055178582661 2215 78-79 -0.7264541603660035 2215 80-81 -0.6699589713226288 2215 82-83 -0.6583278671823862 2215 84-85 -0.6869471076231335 2215 86-87 -0.641164914428991 2215 88-89 -0.6401075656546311 2215 90-91 -0.5622514503181719 2215 92-93 -0.6150152471131705 2215 94-95 -0.6594534686194109 2215 96-97 -0.686405667693478 2215 98-99 -0.6738032438224693 2215 100 -0.742801622868452 2216 1 -0.5427564472170907 2216 2 -0.35638208852751774 2216 3 -0.5665393706532313 2216 4 -0.3992539884614956 2216 5 -0.4576175539622298 2216 6 -0.5087692865035223 2216 7 -0.5911469946876267 2216 8 -0.616731718475819 2216 9 -0.6291363369861145 2216 10-11 -0.7048972010492207 2216 12-13 -0.7188810330523552 2216 14-15 -0.9636421807915028 2216 16-17 -0.9747956063503267 2216 18-19 -1.019479521741843 2216 20-21 -0.9603293585459127 2216 22-23 -0.9128750132181196 2216 24-25 -0.9400891598418752 2216 26-27 -1.0098529096242146 2216 28-29 -1.0802672640688442 2216 30-31 -1.0897323518940212 2216 32-33 -1.1311028620840489 2216 34-35 -1.0359331469667126 2216 36-37 -1.0714067549564348 2216 38-39 -1.1011949831472592 2216 40-41 -1.08000245216504 2216 42-43 -1.1640304668558912 2216 44-45 -1.0696004938337182 2216 46-47 -1.1007478812373535 2216 48-49 -1.2451503083564646 2216 50-51 -1.1452800554030134 2216 52-53 -1.1407505291142215 2216 54-55 -1.1833827134778971 2216 56-57 -1.1288439440238136 2216 58-59 -1.1945573682413269 2216 60-61 -1.1545381989379635 2216 62-63 -1.1595795133845357 2216 64-65 -1.128045574157376 2216 66-67 -1.1631110231926272 2216 68-69 -1.1080022298969538 2216 70-71 -1.1619172123457062 2216 72-73 -1.1492471425641568 2216 74-75 -1.244677535938962 2216 76-77 -1.1086294138559651 2216 78-79 -0.9492765510249654 2216 80-81 -0.9316755664625447 2216 82-83 -0.8788602245938009 2216 84-85 -0.9932094031762837 2216 86-87 -0.8922592857537452 2216 88-89 -0.8864721422027912 2216 90-91 -0.9805175994158581 2216 92-93 -0.9712906411855435 2216 94-95 -0.865215665652304 2216 96-97 -0.9065685741414846 2216 98-99 -0.9480639003725386 2216 100 -0.942255796404698 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 2.0 12 14.0 13 80.0 14 320.0 15 1002.0 16 2331.0 17 4493.0 18 7795.0 19 11776.0 20 15963.0 21 22272.0 22 28978.0 23 32213.0 24 33100.0 25 35883.0 26 42106.0 27 52280.0 28 65619.0 29 82469.0 30 101619.0 31 125094.0 32 154841.0 33 195852.0 34 266351.0 35 365158.0 36 545330.0 37 912704.0 38 755297.0 39 287482.0 40 413.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.519067680164 22.17885213037898 13.207848272390748 25.094231917066274 2 14.83294035295439 21.667737700903285 39.87879877685158 23.620523169290742 3 17.49820368143468 28.6692201988882 30.228793498206574 23.603782621470547 4 12.082220375429362 18.300270678112927 40.07292179748397 29.54458714897374 5 12.299054959024602 39.36670419641387 35.42853933597174 12.90570150858978 6 27.601150008749435 40.058541692878826 19.085006452409083 13.255301845962652 7 24.22774974167335 32.922878979718796 25.83560566783254 17.013765610775312 8 25.151211013782653 34.78639079183135 21.195549211610576 18.86684898277542 9 26.561895161970657 14.340473163811168 22.31465774272218 36.782973931496 10-11 25.107307154436974 26.007944875167453 28.052059395907964 20.832688574487605 12-13 25.811938186065593 24.20629583512299 28.147242673731775 21.83452330507964 14-15 23.023583729392573 24.87325160128139 26.254904061721966 25.84826060760407 16-17 21.687332304982665 26.9280882065999 27.603454033773595 23.781125454643842 18-19 21.128530446356304 27.162472962308964 29.989686268781764 21.719310322552964 20-21 22.640763828421818 26.37114613430871 28.911532447342175 22.076557589927297 22-23 23.114654089217584 25.991569612521786 28.551309161369755 22.342467136890875 24-25 22.61682668737608 26.161240810077423 29.38006979303602 21.84186270951047 26-27 22.45053818165428 26.56614278020209 28.454915558754312 22.52840347938932 28-29 21.75103004744943 27.146287707546062 28.806836998696983 22.29584524630752 30-31 22.53006745503199 26.69385982291909 28.704483038383277 22.071589683665643 32-33 22.957934245685177 26.987773444034207 27.84273572503916 22.211556585241457 34-35 22.87699947129488 26.560797895206473 28.79303743441953 21.76916519907911 36-37 22.824041333983153 26.281997513520654 28.39693909475376 22.497022057742434 38-39 22.981977122268933 26.090112460404573 29.02384956944571 21.90406084788078 40-41 22.397123042594565 25.532297980574032 29.497454401273615 22.57312457555779 42-43 22.50520540390644 25.85018584228961 28.60415393461287 23.040454819191076 44-45 22.478980861629207 26.86142481340558 28.35941051446212 22.300183810503086 46-47 22.537659942374226 26.444585206749455 27.686414846759504 23.331340004116814 48-49 23.52659310358126 25.86627040941762 27.89775164986671 22.709384837134415 50-51 22.96092580150464 26.20440063029704 27.21917886636919 23.615494701829125 52-53 22.18505518894688 26.765301513339395 27.296835885132175 23.752807412581546 54-55 22.470569406451794 26.761961025629162 26.988085071715417 23.77938449620363 56-57 23.499558611297743 25.98932833594953 26.92320439159162 23.58790866116111 58-59 22.25148746575846 26.23280993339094 28.14143006410233 23.37427253674827 60-61 22.554366132681743 27.074210546930317 27.035982971133482 23.335440349254462 62-63 23.08918786416823 26.18269259971105 26.562811081078607 24.165308455042112 64-65 21.87542632419005 26.36823852847472 27.281916044926312 24.474419102408916 66-67 22.8654746220508 25.890381837034855 27.10838552867092 24.13575801224343 68-69 22.075329237759224 25.93231154885614 27.433340463930726 24.559018749453905 70-71 22.0868006888714 25.69033567416238 27.557929191829473 24.664934445136744 72-73 22.736631317009724 25.76492502237976 27.837975355991247 23.66046830461927 74-75 22.644127809372993 26.372535904657575 27.328647680613955 23.654688605355478 76-77 22.217397445576037 26.251119621358278 27.094559676662026 24.436923256403656 78-79 22.00136689484804 26.445787771678187 27.096081186467618 24.456764147006155 80-81 21.646952511273984 26.683697659345274 27.798537907316273 23.870811922064465 82-83 22.509877621619122 25.489542447741165 28.59085772850376 23.409722202135956 84-85 22.846220810025887 26.098386198496588 27.053161406303612 24.002231585173913 86-87 21.32998467044223 26.76563204505509 27.597747775924507 24.306635508578175 88-89 22.19961856478734 25.506659930163416 27.483535179347346 24.810186325701896 90-91 21.87476837023641 25.49177823563052 27.677411073755735 24.956042320377332 92-93 22.148668193322887 25.802121248644088 27.38395805712908 24.665252500903943 94-95 20.81142079083645 25.822418590312417 28.034825621817667 25.331334997033466 96-97 21.231773781386494 25.665507135497624 27.585428498048586 25.517290585067297 98-99 20.69453781239161 26.50141885802477 27.41848827565062 25.385555053932997 100 21.495186874236357 25.540600684849718 26.745878297452496 26.218334143461426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 111.0 1 87.0 2 98.5 3 140.0 4 109.5 5 96.5 6 174.0 7 388.0 8 624.5 9 683.0 10 537.0 11 422.0 12 455.0 13 540.0 14 665.0 15 830.0 16 1053.0 17 1403.5 18 1830.0 19 2390.5 20 3156.5 21 3898.0 22 4638.0 23 5447.5 24 6362.0 25 7370.0 26 8893.5 27 10724.5 28 13443.0 29 20185.5 30 30624.0 31 41064.5 32 55005.0 33 70129.5 34 85676.0 35 99255.5 36 112415.5 37 137969.0 38 146914.0 39 135640.0 40 124477.5 41 114190.5 42 112579.0 43 118604.0 44 129680.5 45 154770.5 46 177782.0 47 193158.0 48 202359.5 49 193535.0 50 181792.5 51 180914.5 52 179492.5 53 173195.5 54 166238.0 55 144896.5 56 127885.5 57 105643.0 58 80598.0 59 75139.0 60 61588.0 61 42563.5 62 30507.0 63 23881.0 64 17265.0 65 8412.5 66 4324.5 67 2952.0 68 2103.0 69 1551.5 70 1227.5 71 980.5 72 758.5 73 684.5 74 588.5 75 431.5 76 304.0 77 174.5 78 92.0 79 52.0 80 35.5 81 19.5 82 9.0 83 3.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013545961543931097 2 0.004820626883961244 3 0.0018077350814854664 4 0.026682169802725485 5 0.003109304340155002 6 0.0 7 5.061658228159307E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.00876148936159956 16-17 0.002976737100846068 18-19 0.0 20-21 1.205156720990311E-5 22-23 8.918159735328302E-4 24-25 0.0 26-27 1.5667037372874042E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 5.4232052444564E-4 36-37 0.0 38-39 0.0 40-41 2.5308291140796533E-4 42-43 5.905267932852524E-4 44-45 0.0 46-47 0.0 48-49 4.338564195565119E-4 50-51 1.205156720990311E-5 52-53 0.0 54-55 2.1692820977825596E-4 56-57 1.205156720990311E-5 58-59 7.230940325941866E-5 60-61 1.205156720990311E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0012172082882002141 70-71 5.543720916555431E-4 72-73 0.0 74-75 7.230940325941866E-5 76-77 0.0027477573238579092 78-79 4.459079867664151E-4 80-81 0.00306109807131539 82-83 0.0015908068717072107 84-85 0.005242431736307853 86-87 0.007628642043868668 88-89 0.007134527788262642 90-91 0.005917319500062427 92-93 0.008146859433894503 94-95 0.0012895176914596328 96-97 0.00430240949393541 98-99 0.0019041476191646914 100 0.08202296643060057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4148838.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.410231256370125 #Duplication Level Percentage of deduplicated Percentage of total 1 68.50961693450024 13.982971249184809 2 15.45152424868816 6.30738366358272 3 5.99351989640383 3.6698738137577305 4 2.936524338753162 2.397405633756456 5 1.652880024342032 1.6867831767927781 6 1.0169986366645762 1.2454306417042285 7 0.7180799736860606 1.025932482645047 8 0.49952378796398716 0.8156316824322379 9 0.3602810935190684 0.6618078392419888 >10 2.0406981813383487 7.987132772878976 >50 0.289650084674008 4.240661898280599 >100 0.38384390578541544 17.69711007495907 >500 0.08133904963532451 11.67171357874045 >1k 0.06304071481170288 22.728786575816272 >5k 0.001888860368889974 2.4115769828878366 >10k+ 5.902688652781169E-4 1.4697979333389088 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 13402 0.323030207494243 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 12664 0.305242094292426 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 12564 0.3028317808504454 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 12214 0.2943956838035132 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 10156 0.24479143316755198 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 8522 0.2054069115255886 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 8274 0.19942933418947667 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 7303 0.17602519066784483 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7191 0.17332563961282652 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6810 0.16414234539888034 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 6721 0.16199716643551762 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 6029 0.1453177974170117 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 5986 0.14428136263696004 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 5982 0.1441849500992808 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5694 0.1372432473863766 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5669 0.13664066902588146 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 5326 0.12837329391988794 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 5236 0.12620401182210536 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5174 0.12470961748807738 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 5164 0.12446858614387933 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 5005 0.12063618777113014 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 4873 0.11745457402771572 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 4822 0.11622531417230558 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 4819 0.11615300476904619 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4764 0.11482733237595684 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 4734 0.11410423834336265 No Hit ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT 4710 0.1135257631172873 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 4686 0.11294728789121196 No Hit CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC 4635 0.11171802803580182 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 4633 0.11166982176696223 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 4535 0.1093077145938212 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 4457 0.10742767010907632 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 4304 0.10373989054284598 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 4280 0.10316141531677063 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 4279 0.10313731218235082 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 4261 0.1027034557627943 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 4238 0.10214908367113876 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 4220 0.10171522725158225 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 4189 0.10096803008456826 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.410313441980622E-5 7 0.0 0.0 0.0 0.0 2.410313441980622E-5 8 0.0 0.0 0.0 0.0 2.410313441980622E-5 9 0.0 0.0 0.0 0.0 2.410313441980622E-5 10-11 0.0 0.0 0.0 0.0 2.410313441980622E-5 12-13 0.0 0.0 0.0 0.0 1.6872194093864356E-4 14-15 0.0 0.0 0.0 0.0 2.5308291140796533E-4 16-17 0.0 0.0 0.0 0.0 3.25392314667384E-4 18-19 0.0 0.0 0.0 0.0 3.374438818772871E-4 20-21 0.0 0.0 0.0 0.0 4.0975328513670576E-4 22-23 0.0 0.0 0.0 6.025783604951555E-5 4.0975328513670576E-4 24-25 0.0 0.0 0.0 2.1692820977825599E-4 4.0975328513670576E-4 26-27 0.0 0.0 0.0 7.110424653842835E-4 4.3385641955651197E-4 28-29 0.0 0.0 0.0 0.002410313441980622 4.3385641955651197E-4 30-31 0.0 0.0 0.0 0.010207677426787933 4.4590798676641505E-4 32-33 0.0 0.0 0.0 0.03073149638525293 4.579595539763182E-4 34-35 0.0 0.0 0.0 0.061595560009814795 4.579595539763182E-4 36-37 0.0 0.0 0.0 0.11254958617328514 4.579595539763182E-4 38-39 0.0 0.0 0.0 0.1995377982943658 5.543720916555431E-4 40-41 0.0 0.0 0.0 0.3068811074329728 5.543720916555431E-4 42-43 0.0 0.0 0.0 0.4185027229310954 5.664236588654462E-4 44-45 0.0 0.0 0.0 0.5454659834874247 5.905267932852524E-4 46-47 0.0 0.0 0.0 0.7017145523638185 6.146299277050586E-4 48-49 0.0 0.0 0.0 0.8875858734421542 6.748877637545742E-4 50-51 0.0 0.0 0.0 1.0926071348170259 6.748877637545742E-4 52-53 0.0 0.0 0.0 1.2996771626175811 7.230940325941867E-4 54-55 0.0 0.0 0.0 1.5161835675434905 7.230940325941867E-4 56-57 0.0 0.0 0.0 1.77620818166436 7.351455998040897E-4 58-59 0.0 0.0 0.0 2.0905612607674726 7.471971670139928E-4 60-61 0.0 0.0 0.0 2.4336573276662046 7.471971670139928E-4 62-63 0.0 0.0 0.0 2.803218636157883 8.195065702734115E-4 64-65 0.0 0.0 0.0 3.1839637990203524 8.195065702734115E-4 66-67 0.0 0.0 0.0 3.595874314687631 8.195065702734115E-4 68-69 0.0 0.0 0.0 4.05248409313644 8.195065702734115E-4 70-71 0.0 0.0 0.0 4.56982171875595 8.195065702734115E-4 72-73 0.0 0.0 0.0 5.128905973190566 8.315581374833146E-4 74-75 0.0 0.0 0.0 5.673504243838877 8.436097046932177E-4 76-77 0.0 0.0 0.0 6.279228063375818 8.677128391130239E-4 78-79 0.0 0.0 0.0 6.919154230654463 9.038675407427333E-4 80-81 0.0 0.0 0.0 7.626605329010195 9.641253767922488E-4 82-83 0.0 0.0 0.0 8.395977379690411 0.0010364347800516675 84-85 0.0 0.0 0.0 9.145295622533347 0.0010364347800516675 86-87 0.0 0.0 0.0 9.924284823847064 0.0010364347800516675 88 0.0 0.0 0.0 10.527333195463404 0.0010364347800516675 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13585 0.0 40.567368 1 GGTATCA 7610 0.0 39.298985 1 TACCCCG 345 0.0 38.143684 5 CAACGCA 16010 0.0 33.230648 5 ATCAACG 16375 0.0 32.554005 3 TATTCTC 2570 0.0 31.6371 5 TATCAAC 17730 0.0 31.130005 2 AACGCAG 19175 0.0 29.337393 6 GGGCACC 3460 0.0 28.00166 94 ACGCAGA 19325 0.0 27.455996 7 CGCAGAG 19585 0.0 27.067509 8 GTGGTAT 2075 0.0 26.287466 1 TCAACGC 20540 0.0 26.204615 4 GTACATG 17935 0.0 25.405704 1 TACATGG 17965 0.0 25.174896 2 ACATGGG 17770 0.0 25.025135 3 GCAGAGT 21145 0.0 24.763885 9 CTATTGA 5475 0.0 24.124788 9 AGGGCTA 5490 0.0 24.055683 5 GCTATTG 5580 0.0 23.750769 8 >>END_MODULE