FastQCFastQC Report
Mon 16 Jan 2017
ERR1146416_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146416_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4645350
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC206790.44515483225160646No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT177450.3819948981239304No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN141190.30393834694909966No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT87480.18831734960767219No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG86120.18538969076603484No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT74890.16121497841927948No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG69440.1494828161494828No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT65670.14136717362523812No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA61520.1324335087775948No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG61000.13131410980873345No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC60500.1302377646463668No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT60450.13013013013013014No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC58450.12582474948066347No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG57160.12304777896175746No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT53520.11521198617972811No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA53390.11493213643751278No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA52240.11245654256406945No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA51090.10998094869062612No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA50630.10899071114124877No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC49990.10761298933341945No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA47410.10205904829560743No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG46940.10104728384298277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC58850.037.54841294
GTACTAG60900.037.0997661
TAGGCAT74100.033.032285
GCATCGT66350.032.1266948
CAACGCA76250.031.5463525
CTAGGCA82000.030.7106344
GTATCAA98400.028.5695971
TAGTACT84100.028.435424
CTGTGCG83900.028.0941819
ATCAACG87350.027.6487643
CTAACGC27000.027.321933
GTACTGT89650.026.917566
CTAGTAC94500.026.7501323
TCTAACG28650.026.2409922
CGTTAAC9350.026.2328011
TACTGTG94350.026.1251667
TAACGCC28600.026.1197454
GTACATG190800.025.8586751
ACTGTGC96200.025.8185428
CGCCTAC28700.025.683817
Produced by FastQC (version 0.11.5)