##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146416_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4645350
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.431578890718676	31.0	30.0	34.0	27.0	34.0
2	30.56460331299041	31.0	31.0	34.0	26.0	34.0
3	30.79542983844059	31.0	31.0	34.0	27.0	34.0
4	34.27615421873487	37.0	35.0	37.0	30.0	37.0
5	34.44258968646066	37.0	35.0	37.0	30.0	37.0
6	34.65820207304078	37.0	35.0	37.0	31.0	37.0
7	34.562538237161895	37.0	35.0	37.0	30.0	37.0
8	34.53760448620664	37.0	35.0	37.0	30.0	37.0
9	35.97894173743636	39.0	35.0	39.0	30.0	39.0
10-11	35.90947668098205	39.0	35.0	39.0	30.0	39.0
12-13	35.903538161817735	39.0	35.0	39.0	30.0	39.0
14-15	36.93926862346217	40.0	36.0	41.0	30.0	41.0
16-17	36.722799250863766	40.0	36.0	41.0	29.5	41.0
18-19	36.64472644687699	40.0	36.0	41.0	28.5	41.0
20-21	36.966073600482204	40.0	36.0	41.0	30.0	41.0
22-23	36.64535923019794	40.0	36.0	41.0	29.0	41.0
24-25	36.72337929327176	39.5	36.0	41.0	29.5	41.0
26-27	36.77644924494387	40.0	36.0	41.0	30.0	41.0
28-29	36.57988956698634	39.5	35.5	41.0	29.5	41.0
30-31	36.515212416717794	39.0	35.0	41.0	29.0	41.0
32-33	36.61889405534566	39.0	35.0	41.0	30.0	41.0
34-35	36.659347734831606	39.0	35.0	41.0	30.0	41.0
36-37	36.58106568934526	39.0	35.0	41.0	29.5	41.0
38-39	36.40084127137891	39.0	35.0	41.0	29.0	41.0
40-41	36.35526235913332	39.0	35.0	41.0	29.0	41.0
42-43	36.173698967784986	39.0	35.0	41.0	28.0	41.0
44-45	35.97586855672877	39.0	35.0	41.0	28.0	41.0
46-47	35.825834651856155	38.0	34.0	40.0	27.5	41.0
48-49	35.65263866016554	38.0	34.0	40.0	27.0	41.0
50-51	35.410943631803846	38.0	34.0	40.0	27.0	41.0
52-53	35.19302711313464	37.5	33.5	40.0	26.5	41.0
54-55	34.937268020708885	37.0	33.0	40.0	26.0	41.0
56-57	34.74951618284952	37.0	33.0	40.0	26.0	41.0
58-59	34.445737673157026	36.0	33.0	39.5	26.0	41.0
60-61	34.15371037704371	35.5	33.0	39.0	26.0	41.0
62-63	33.83871764237355	35.0	32.5	39.0	26.0	40.5
64-65	33.51570796603055	35.0	32.0	38.0	25.5	40.0
66-67	33.19962715403575	35.0	32.0	37.0	25.0	40.0
68-69	32.87739502943804	35.0	31.5	37.0	25.0	39.5
70-71	32.80355301538097	35.0	32.0	36.0	25.0	39.0
72-73	32.587910275867266	35.0	31.5	36.0	25.0	39.0
74-75	32.34001474592873	35.0	31.0	35.5	25.0	38.5
76-77	32.131159331374384	35.0	31.0	35.0	24.0	37.0
78-79	31.9430619867179	35.0	31.0	35.0	24.0	37.0
80-81	31.7646549775582	34.5	31.0	35.0	24.0	36.5
82-83	31.578881031569203	34.0	31.0	35.0	23.5	36.0
84-85	31.450134220241747	34.0	31.0	35.0	23.0	36.0
86-87	31.343770329469255	34.0	31.0	35.0	23.0	36.0
88-89	31.417416663975803	34.5	31.0	35.0	23.5	35.0
90-91	31.384515590859678	35.0	31.0	35.0	23.0	35.0
92-93	31.26817494914269	34.5	31.0	35.0	23.0	35.0
94-95	31.26986158201212	34.0	31.0	35.0	23.0	35.0
96-97	31.19244911578245	34.0	31.0	35.0	23.0	35.0
98-99	31.126470556578084	34.0	31.0	35.0	23.0	35.0
100	30.608117364676506	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6836180589683885
1101	2	0.7758967797500453
1101	3	0.7344974174315411
1101	4	0.8119877966049032
1101	5	0.7954074233590802
1101	6	0.4540317666947047
1101	7	0.5228435790454284
1101	8	0.5571704254619121
1101	9	0.696242149965073
1101	10-11	0.6602280467758135
1101	12-13	0.5531366590537843
1101	14-15	0.7902530313577572
1101	16-17	0.9621415934771278
1101	18-19	1.089274592119871
1101	20-21	0.7439356971767808
1101	22-23	1.0912185515280015
1101	24-25	0.9682117268340491
1101	26-27	0.8520106462029275
1101	28-29	1.0597296399366911
1101	30-31	1.0362375488693516
1101	32-33	0.8419228724127166
1101	34-35	0.7612665164145866
1101	36-37	0.7488100294958997
1101	38-39	0.8363079518679655
1101	40-41	0.7271921071972116
1101	42-43	0.7459788150545918
1101	44-45	0.7906524212204573
1101	46-47	0.7778161956758751
1101	48-49	0.7599684150247725
1101	50-51	0.8055626798972426
1101	52-53	0.7419194095586974
1101	54-55	0.716962172951483
1101	56-57	0.6737424130775693
1101	58-59	0.5835067479010618
1101	60-61	0.6546286777253769
1101	62-63	0.5431324528678019
1101	64-65	0.5842145350823387
1101	66-67	0.5700273184967912
1101	68-69	0.5813566401672929
1101	70-71	0.47248156381288453
1101	72-73	0.5128107340808228
1101	74-75	0.5937538014378774
1101	76-77	0.5698144210983642
1101	78-79	0.5347376457451141
1101	80-81	0.5321388981731126
1101	82-83	0.47003443634052644
1101	84-85	0.5229480024817654
1101	86-87	0.5299699846949757
1101	88-89	0.5329237929743194
1101	90-91	0.6000246524613502
1101	92-93	0.630460760447388
1101	94-95	0.5828012738441046
1101	96-97	0.5691651408617133
1101	98-99	0.5634777808578804
1101	100	0.6203416054515216
1102	1	0.5263226868270721
1102	2	0.6901853143410008
1102	3	0.6446800770644607
1102	4	0.7758494890689107
1102	5	0.5713725140403412
1102	6	0.4351719077204521
1102	7	0.5021987698609323
1102	8	0.5135833363298303
1102	9	0.5936808320713922
1102	10-11	0.613338228701231
1102	12-13	0.5406154943819317
1102	14-15	0.7043571462472045
1102	16-17	0.9144271638923129
1102	18-19	0.9299916477351857
1102	20-21	0.7132947997090326
1102	22-23	0.9706397806065326
1102	24-25	0.8483340261077075
1102	26-27	0.7651875129055696
1102	28-29	0.8951384469691277
1102	30-31	0.9305703625439605
1102	32-33	0.8136007582419822
1102	34-35	0.6683044045933357
1102	36-37	0.5320043010946733
1102	38-39	0.6303229742471359
1102	40-41	0.5962793066649894
1102	42-43	0.6333466110612136
1102	44-45	0.7623865966130197
1102	46-47	0.6607387987500104
1102	48-49	0.6340339266948689
1102	50-51	0.7142389308818053
1102	52-53	0.5905352063680525
1102	54-55	0.6573323543303218
1102	56-57	0.5949559406017357
1102	58-59	0.5933277980766789
1102	60-61	0.6007209175564441
1102	62-63	0.6190375523351364
1102	64-65	0.4890724773561672
1102	66-67	0.5357107737296616
1102	68-69	0.5905712518773996
1102	70-71	0.5133471749278726
1102	72-73	0.46612909561400073
1102	74-75	0.47636410960691933
1102	76-77	0.48431332773477465
1102	78-79	0.523454647518232
1102	80-81	0.5393201513827357
1102	82-83	0.5968202533284668
1102	84-85	0.5240197985960684
1102	86-87	0.5169603652980825
1102	88-89	0.48840731420466454
1102	90-91	0.47834242254621984
1102	92-93	0.5288720691022348
1102	94-95	0.48444647909502336
1102	96-97	0.515335084763862
1102	98-99	0.5166623017174459
1102	100	0.5349795024408053
1103	1	0.5296632707438356
1103	2	0.683425513085961
1103	3	0.6164085875298966
1103	4	0.593565172609722
1103	5	0.6378281595968787
1103	6	0.3538849127511341
1103	7	0.3959908168753259
1103	8	0.43488248090715587
1103	9	0.5206466413867687
1103	10-11	0.6067577295799893
1103	12-13	0.4527547842849131
1103	14-15	0.5725666351560221
1103	16-17	0.822829699881062
1103	18-19	0.9022495634629664
1103	20-21	0.5173799345310144
1103	22-23	0.8146186209077086
1103	24-25	0.7386917482869677
1103	26-27	0.67471093042235
1103	28-29	0.8262807094116909
1103	30-31	0.911537919389005
1103	32-33	0.6192448412478839
1103	34-35	0.586959559584237
1103	36-37	0.638644737898602
1103	38-39	0.6819401649612402
1103	40-41	0.6111529080076821
1103	42-43	0.5968015846132815
1103	44-45	0.6921566821654395
1103	46-47	0.5980377891056818
1103	48-49	0.5478886534110785
1103	50-51	0.6620220503636958
1103	52-53	0.6867197665874656
1103	54-55	0.662726170569627
1103	56-57	0.5930497991318902
1103	58-59	0.6268432131202175
1103	60-61	0.5784395082330533
1103	62-63	0.4999586718138431
1103	64-65	0.4954437638683089
1103	66-67	0.5262815809771908
1103	68-69	0.5620369121986784
1103	70-71	0.5149784631527652
1103	72-73	0.45735635151432774
1103	74-75	0.41066899878877194
1103	76-77	0.4692747789179563
1103	78-79	0.44332417119018785
1103	80-81	0.5048461969610933
1103	82-83	0.42369092770784533
1103	84-85	0.42921679612571495
1103	86-87	0.5561268554286585
1103	88-89	0.5755666422651551
1103	90-91	0.5494967449330304
1103	92-93	0.6131963476482056
1103	94-95	0.5799052196752115
1103	96-97	0.5485288183974752
1103	98-99	0.5958023826577659
1103	100	0.5368990511797058
1104	1	0.38074973063583784
1104	2	0.5475657094098061
1104	3	0.5118022481510245
1104	4	0.6225321111893081
1104	5	0.46912266606663167
1104	6	0.20008262792698162
1104	7	0.2531650967183623
1104	8	0.2793698327673724
1104	9	0.3816358552678736
1104	10-11	0.39530154163875153
1104	12-13	0.3538270877645715
1104	14-15	0.4917610048628518
1104	16-17	0.5644500005889697
1104	18-19	0.5836821627258715
1104	20-21	0.4304297597488471
1104	22-23	0.7656133087834434
1104	24-25	0.6470637172197229
1104	26-27	0.4684926661986637
1104	28-29	0.6561186552325466
1104	30-31	0.7464901099979073
1104	32-33	0.5190982747260264
1104	34-35	0.3735029578795306
1104	36-37	0.4060145177449854
1104	38-39	0.4939015269407534
1104	40-41	0.4099750460791256
1104	42-43	0.33416910502602093
1104	44-45	0.4186555117167501
1104	46-47	0.41964173432372576
1104	48-49	0.3659151847408708
1104	50-51	0.45093917535337624
1104	52-53	0.5081992400623605
1104	54-55	0.48061875049198477
1104	56-57	0.4176315609465888
1104	58-59	0.40310658042723446
1104	60-61	0.38956990773304057
1104	62-63	0.3672511804613876
1104	64-65	0.38170578833655355
1104	66-67	0.4663726771411163
1104	68-69	0.4226038206752136
1104	70-71	0.26968778476241084
1104	72-73	0.30780714535607956
1104	74-75	0.40153674031979136
1104	76-77	0.3013685608958383
1104	78-79	0.31787076276836856
1104	80-81	0.3417115747821491
1104	82-83	0.26648899827562644
1104	84-85	0.24220135658283581
1104	86-87	0.2584160545480856
1104	88-89	0.11670016732640676
1104	90-91	0.1765104276690579
1104	92-93	0.1943634924411768
1104	94-95	0.13326786972286442
1104	96-97	0.19399202497303492
1104	98-99	0.22288714582625602
1104	100	0.29053040197083035
1105	1	0.20467050258372765
1105	2	0.4155634436156852
1105	3	0.36539910981163004
1105	4	0.46505204934710775
1105	5	0.4182039259976449
1105	6	0.08078240812972837
1105	7	0.1407349944600398
1105	8	0.14213101502192416
1105	9	0.22016186414834493
1105	10-11	0.2508413875038542
1105	12-13	0.22344087851980277
1105	14-15	0.38056470155371613
1105	16-17	0.5063865348236405
1105	18-19	0.505711854732894
1105	20-21	0.3148622696729433
1105	22-23	0.626352629654221
1105	24-25	0.524323006006739
1105	26-27	0.32623000017422044
1105	28-29	0.61073688899274
1105	30-31	0.5932542597922321
1105	32-33	0.4513813079835103
1105	34-35	0.3348963243762242
1105	36-37	0.27849856237435233
1105	38-39	0.35588821107494084
1105	40-41	0.22863256998277137
1105	42-43	0.25084860934721576
1105	44-45	0.3169449548047609
1105	46-47	0.2794482836729131
1105	48-49	0.357992466037075
1105	50-51	0.39457768499512014
1105	52-53	0.32409784473734504
1105	54-55	0.3150453606314443
1105	56-57	0.35360436449656163
1105	58-59	0.29809928960833787
1105	60-61	0.3090943539491491
1105	62-63	0.29368295957345936
1105	64-65	0.17720899325181705
1105	66-67	0.33414435097748907
1105	68-69	0.2964500754019568
1105	70-71	0.17068509058509562
1105	72-73	0.16718422152662527
1105	74-75	0.1140772429468413
1105	76-77	0.13465320802989567
1105	78-79	0.14795684425944344
1105	80-81	0.27884317562037
1105	82-83	0.2575997702788797
1105	84-85	0.14872108573947074
1105	86-87	0.23901316811738127
1105	88-89	0.25737297115320246
1105	90-91	0.23599525709088454
1105	92-93	0.3067700301638112
1105	94-95	0.2869600276114852
1105	96-97	0.22474895177002807
1105	98-99	0.276476083435945
1105	100	0.35888267690010167
1106	1	0.3832318046762211
1106	2	0.4326303286165647
1106	3	0.3946615724457061
1106	4	0.5689908571543825
1106	5	0.43023575297144845
1106	6	0.20521367681268998
1106	7	0.19087786416287145
1106	8	0.21284657243356264
1106	9	0.2788703220347557
1106	10-11	0.301551340027153
1106	12-13	0.28330163550942444
1106	14-15	0.40383625289889125
1106	16-17	0.4989122513701503
1106	18-19	0.5220102655822032
1106	20-21	0.3236809020801914
1106	22-23	0.6991615029617648
1106	24-25	0.5016270547143193
1106	26-27	0.34848470388443786
1106	28-29	0.5529892298578716
1106	30-31	0.544700266034539
1106	32-33	0.40196789801340316
1106	34-35	0.4033408030117087
1106	36-37	0.26750336499436145
1106	38-39	0.3676700160687858
1106	40-41	0.35542860699701606
1106	42-43	0.377221532241542
1106	44-45	0.39712782802438795
1106	46-47	0.38012554101421614
1106	48-49	0.32212035640105796
1106	50-51	0.3355211195827721
1106	52-53	0.3324048937086843
1106	54-55	0.3538276162143461
1106	56-57	0.26530539315842816
1106	58-59	0.2249219773171447
1106	60-61	0.27537051009474567
1106	62-63	0.2568723058896225
1106	64-65	0.2855569798880069
1106	66-67	0.34000255829241866
1106	68-69	0.21684967097080232
1106	70-71	0.2331441529202536
1106	72-73	0.21549478729064475
1106	74-75	0.257630797105449
1106	76-77	0.27430724354949376
1106	78-79	0.28867883080771506
1106	80-81	0.2997892478025399
1106	82-83	0.31657995535009675
1106	84-85	0.24179245465102284
1106	86-87	0.3218688037776616
1106	88-89	0.3571833940475244
1106	90-91	0.3225061591482117
1106	92-93	0.4002024198244385
1106	94-95	0.3494380476572019
1106	96-97	0.29024536903747844
1106	98-99	0.29187771215055847
1106	100	0.32349604950428557
1107	1	0.3779050108688544
1107	2	0.5715956351470695
1107	3	0.4280481965568299
1107	4	0.5983050468398545
1107	5	0.5306598915685043
1107	6	0.23298115310472411
1107	7	0.27756195928852634
1107	8	0.3167610557822229
1107	9	0.423780828892788
1107	10-11	0.38347466387548224
1107	12-13	0.23871180844290052
1107	14-15	0.4669112271234539
1107	16-17	0.597121708440099
1107	18-19	0.7278138115073673
1107	20-21	0.42612077015618866
1107	22-23	0.71231225629829
1107	24-25	0.4031913824582176
1107	26-27	0.3583455437149041
1107	28-29	0.5560578588357217
1107	30-31	0.5184648896859159
1107	32-33	0.46212509588988837
1107	34-35	0.3201742052609049
1107	36-37	0.2776209815891235
1107	38-39	0.3811023864842866
1107	40-41	0.15511271334416676
1107	42-43	0.3380652307246663
1107	44-45	0.2810732657035899
1107	46-47	0.2908619551813203
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2214	1	-0.7805242877725718
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2214	4	-0.7422193584739176
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2214	92-93	-0.9440125514145734
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2215	1	-3.912313611607832
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2215	3	-3.721227431227387
2215	4	-1.7073808141036508
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2215	9	-1.4187019920148316
2215	10-11	-1.0268462724613698
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2215	14-15	-3.4004303716802298
2215	16-17	-3.10913794758914
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2215	20-21	-1.4486326692390605
2215	22-23	-2.936788431993847
2215	24-25	-5.34720692906285
2215	26-27	-3.3894055624149217
2215	28-29	-1.4034908168210336
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2215	100	-2.815507623590296
2216	1	0.48912210679959855
2216	2	0.5472022333228104
2216	3	0.3306632149757682
2216	4	0.36617799066072365
2216	5	0.21918187435120018
2216	6	0.2749680106463046
2216	7	0.11442568980569234
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2216	9	0.2810025539308256
2216	10-11	0.29946140530599763
2216	12-13	0.11308598621770471
2216	14-15	0.08848937370601107
2216	16-17	0.38408002566210797
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2216	24-25	0.4196668857613588
2216	26-27	0.5075393933335448
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2216	30-31	0.30066320081757425
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2216	34-35	0.2691833725583379
2216	36-37	0.2678746623647399
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2216	40-41	0.10660391902615629
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2216	54-55	0.36755235884616866
2216	56-57	0.2802806803126572
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2216	62-63	0.14220860894709375
2216	64-65	0.17448014783713006
2216	66-67	0.16638778770149543
2216	68-69	0.1053530594103762
2216	70-71	0.06385770626109633
2216	72-73	0.05849134200465045
2216	74-75	-0.0027376432104730952
2216	76-77	0.12971346095181957
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2216	80-81	0.0062471761716302865
2216	82-83	-0.11563401813859997
2216	84-85	0.033337151162346146
2216	86-87	0.1255940123536874
2216	88-89	0.20568145144693872
2216	90-91	0.061819001331741674
2216	92-93	0.12033073066803723
2216	94-95	0.32052538094862726
2216	96-97	0.2427120003112151
2216	98-99	0.23854746649053027
2216	100	0.262945402903874
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	18971.0
1	3180.0
2	492.0
3	467.0
4	399.0
5	233.0
6	234.0
7	296.0
8	549.0
9	978.0
10	1839.0
11	3430.0
12	6077.0
13	8524.0
14	10848.0
15	13325.0
16	15833.0
17	18627.0
18	20816.0
19	23766.0
20	27138.0
21	30941.0
22	35408.0
23	41292.0
24	46349.0
25	53784.0
26	62547.0
27	72793.0
28	86467.0
29	103027.0
30	123519.0
31	150502.0
32	186564.0
33	236155.0
34	323625.0
35	446024.0
36	684236.0
37	1008833.0
38	657159.0
39	120083.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47606074475267	19.217545488444298	11.868752371313118	24.437641395489912
2	16.438064109621976	19.113810298726467	38.97565844412452	25.472467147527034
3	19.932828650546757	24.913488822007388	27.52279746369429	27.63088506375157
4	13.20541382367247	15.61337196539724	37.6796279591236	33.501586251806685
5	14.273968491993278	37.77646472796519	33.564450278940214	14.385116501101319
6	32.21417539487859	36.161165838189554	17.16284442367844	14.461814343253426
7	28.617204596517453	30.53399953917998	22.209837533898938	18.63895833040363
8	27.44809737803568	33.88822191966411	18.996882132222602	19.6667985700776
9	26.50826613038465	14.65230926490804	20.36460858921087	38.47481601549644
10-11	27.212517814678005	24.526176363878545	25.999956501401204	22.26134932004224
12-13	27.18394369742016	22.075132937067636	26.23191920083785	24.509004164674355
14-15	24.508792131238927	23.263414229842112	23.567146727975093	28.66064691094387
16-17	23.97471838380481	26.027283421932175	24.74401773881218	25.253980455450844
18-19	23.329910082589755	26.769730643800173	26.13853745354076	23.761821820069308
20-21	25.170071192340508	26.06843964935649	25.161501493475612	23.599987664827392
22-23	25.614109967660003	25.290266558603484	25.261040610331253	23.834582863405252
24-25	25.08120988060941	25.266665526954803	25.654618397416517	23.997506195019273
26-27	24.887849638245577	25.700239294492334	24.43264920206321	24.97926186519888
28-29	24.012013368718698	25.993650150718857	25.58328029531224	24.411056185250203
30-31	24.55989455450349	25.836407799184446	25.5604388542377	24.043258792074365
32-33	24.819403080509726	25.818037588307725	24.58442070878577	24.778138622396778
34-35	24.84761159793332	25.97912652470909	25.289230390012378	23.884031487345215
36-37	24.58470049322735	25.23144585722143	24.647373938279806	25.536479711271415
38-39	24.71132099564336	25.552360876349685	25.2243032725683	24.512014855438665
40-41	24.445305381263946	24.531057574308107	26.15885582939599	24.864781215031957
42-43	24.409532067886836	24.721765283687677	25.528942200007815	25.33976044841767
44-45	24.79189161664962	25.22375229396223	25.4679834463508	24.516372643037347
46-47	24.598597647218742	25.374557239614575	24.843628974453907	25.183216138712773
48-49	25.254924100255632	25.22993127622556	25.068549041059963	24.446595582458848
50-51	24.55657616449785	24.912092187012206	25.625472408119492	24.905859240370447
52-53	24.606446776611694	25.14221021706237	25.241280197083903	25.01006280924203
54-55	24.19199640560248	25.05216940762563	25.324389614684396	25.431444572087496
56-57	25.331072820602046	24.747950991062577	24.800622712180314	25.120353476155067
58-59	23.992246422155997	25.28318207457982	25.676530938296967	25.048040564967216
60-61	24.12120713748195	25.54444047112185	25.249586272525086	25.08476611887111
62-63	24.610250097319817	25.144107980311425	24.7104694915632	25.53517243080556
64-65	23.884378880522434	25.28592241140885	25.293399416642103	25.536299291426612
66-67	24.578303501588493	25.20272886913415	25.118989562288725	25.099978066988633
68-69	24.09259465288423	24.91620104476507	25.64801062167721	25.343193680673494
70-71	23.79632036257498	24.84192422534376	25.417082636563464	25.94467277551779
72-73	24.389980497988443	24.80059187771843	25.79884987693021	25.010577747362923
74-75	24.608563816566225	24.963682517518688	25.524159336171063	24.90359432974402
76-77	24.38812589498581	24.965331218457415	24.79128674006339	25.85525614649339
78-79	24.52587554146381	25.034806068723803	24.66890943582133	25.77040895399105
80-81	23.9980549978149	25.51247550759028	25.424260093621083	25.065209400973735
82-83	24.853429905222423	24.630527587421344	26.400589039472894	24.115453467883338
84-85	24.570223362223892	24.95219293537541	25.499463712511815	24.97811998988888
86-87	23.42892652362332	25.544954994803437	25.97129544954995	25.054823032023293
88-89	24.52746609287258	24.205664692889012	25.785154819375126	25.481714394863282
90-91	24.09820348072888	24.70686307798121	25.307208277831467	25.88772516345844
92-93	24.38024848392246	24.652861665732924	25.783058238881846	25.18383161146277
94-95	23.646275510892615	24.613535634169416	26.46554992867115	25.274638926266825
96-97	23.90768858085623	24.42346444454819	26.005811905428068	25.663035069167506
98-99	23.364616565396894	24.819530069217187	25.882821891682458	25.933031473703466
100	24.077570258002414	24.11976869424458	25.214621159675705	26.588039888077297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15348.0
1	9003.0
2	2548.0
3	1841.0
4	883.5
5	414.5
6	292.0
7	264.5
8	266.5
9	246.0
10	223.0
11	236.0
12	225.5
13	202.5
14	182.0
15	197.5
16	199.5
17	214.0
18	258.5
19	289.5
20	363.5
21	451.0
22	553.0
23	721.5
24	966.0
25	1321.5
26	1831.0
27	2420.5
28	3287.5
29	5064.5
30	7829.0
31	10994.0
32	16028.5
33	22304.0
34	28517.0
35	35928.5
36	45766.0
37	62431.0
38	78779.0
39	91207.5
40	97147.0
41	100038.5
42	108552.5
43	123515.0
44	144675.5
45	176572.0
46	209521.5
47	234031.5
48	251546.0
49	254849.0
50	248422.5
51	257298.0
52	264353.5
53	255187.0
54	242812.5
55	214452.5
56	191004.0
57	163578.0
58	134535.0
59	126701.5
60	106569.0
61	77692.5
62	59817.5
63	49180.0
64	36351.0
65	20650.5
66	13123.5
67	9779.5
68	7533.0
69	5718.5
70	4261.5
71	3270.5
72	2366.0
73	1882.0
74	1594.0
75	1337.0
76	1009.0
77	656.5
78	421.5
79	258.5
80	172.5
81	98.0
82	68.0
83	53.5
84	43.0
85	36.5
86	32.5
87	26.5
88	18.0
89	19.0
90	17.0
91	9.5
92	9.0
93	8.5
94	6.5
95	6.5
96	5.0
97	3.5
98	4.0
99	4.0
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9924978742183044
2	0.4431097764431098
3	0.47893054344667246
4	0.46405545330276515
5	0.4302366883012044
6	0.4824179017727405
7	0.49845544469200387
8	0.5011678345011679
9	0.5145145145145146
10-11	0.5276566889470116
12-13	0.5265588168813976
14-15	0.5240294057498359
16-17	0.5278827214311085
18-19	0.5273768392047962
20-21	0.5256331600417622
22-23	0.4896401778122208
24-25	0.5230822220069532
26-27	0.5043753430850205
28-29	0.5094449287997675
30-31	0.5126739642868675
32-33	0.5153433002895369
34-35	0.5276136351405168
36-37	0.5461590622880945
38-39	0.5342977385988139
40-41	0.5394641953781739
42-43	0.5365688268914075
44-45	0.5455240186422983
46-47	0.5452441689000829
48-49	0.5171515601623129
50-51	0.5326294035971455
52-53	0.5246967397505032
54-55	0.534426900018298
56-57	0.5225548128773935
58-59	0.5277427965600008
60-61	0.5220812210059522
62-63	0.5164088820002798
64-65	0.5277535600116245
66-67	0.5265803437846449
68-69	0.5460083739653632
70-71	0.5300569386590892
72-73	0.5338887274371146
74-75	0.5304551863691649
76-77	0.5191750890675622
78-79	0.5482041180965912
80-81	0.5225763397806409
82-83	0.5224041245546621
84-85	0.5315853487896499
86-87	0.8382037951930426
88-89	1.1057832025573961
90-91	1.095934644321741
92-93	1.0963221285801932
94-95	1.1007997244556385
96-97	1.093900351964868
98-99	1.0894658098959173
100	1.0848052353428699
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4645350.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.553292386612192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.38162703792163	22.42050306694946
2	12.502681201461336	7.639961487296959
3	5.165135393175771	4.734356756524154
4	2.6125294667867442	3.1928550666950177
5	1.557964998971343	2.3800480070839702
6	0.984162576913449	1.804164418103899
7	0.6750723934582772	1.4437978953622597
8	0.45911135010049364	1.1221890654106124
9	0.35103129714605136	0.9652645672699545
>10	1.85062504434642	10.354361166118476
>50	0.18379898216007237	3.9581913765287675
>100	0.20856164561837567	14.118900767125256
>500	0.03871514370601127	8.308243260703764
>1k	0.02764359987267737	14.262823611763329
>5k	0.0011283101988847906	2.162248291176666
>10k+	2.1155816229089826E-4	1.1320911958875548
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	20679	0.44515483225160646	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	17745	0.3819948981239304	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	14119	0.30393834694909966	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8748	0.18831734960767219	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	8612	0.18538969076603484	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7489	0.16121497841927948	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6944	0.1494828161494828	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6567	0.14136717362523812	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6152	0.1324335087775948	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6100	0.13131410980873345	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6050	0.1302377646463668	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6045	0.13013013013013014	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5845	0.12582474948066347	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5716	0.12304777896175746	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5352	0.11521198617972811	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5339	0.11493213643751278	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	5224	0.11245654256406945	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	5109	0.10998094869062612	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5063	0.10899071114124877	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4999	0.10761298933341945	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	4741	0.10205904829560743	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	4694	0.10104728384298277	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.305380649466671E-5
2	0.0	0.0	0.0	0.0	4.305380649466671E-5
3	0.0	0.0	0.0	0.0	6.458070974200007E-5
4	0.0	0.0	0.0	0.0	6.458070974200007E-5
5	0.0	0.0	0.0	0.0	6.458070974200007E-5
6	0.0	0.0	0.0	0.0	8.610761298933341E-5
7	0.0	0.0	0.0	0.0	8.610761298933341E-5
8	0.0	0.0	0.0	0.0	8.610761298933341E-5
9	0.0	0.0	0.0	0.0	8.610761298933341E-5
10-11	0.0	0.0	0.0	0.0	9.687106461300009E-5
12-13	0.0	0.0	0.0	0.0	2.906131938390003E-4
14-15	0.0	0.0	0.0	0.0	4.0901116169933375E-4
16-17	0.0	0.0	0.0	1.0763451623666677E-5	5.274091295596672E-4
18-19	0.0	0.0	0.0	2.1526903247333353E-5	6.242801941726673E-4
20-21	0.0	0.0	0.0	2.1526903247333353E-5	7.103878071620007E-4
22-23	0.0	0.0	0.0	3.229035487100003E-5	7.319147104093341E-4
24-25	0.0	0.0	0.0	1.1839796786033345E-4	7.319147104093341E-4
26-27	0.0	0.0	0.0	2.798497422153336E-4	7.426781620330008E-4
28-29	0.0	0.0	0.0	0.0014745928724423348	7.534416136566675E-4
30-31	0.0	0.0	0.0	0.00651188823231834	7.534416136566675E-4
32-33	0.0	0.0	0.0	0.017479845436834684	7.964954201513341E-4
34-35	0.0	0.0	0.0	0.034023270582410364	8.826030331406675E-4
36-37	0.0	0.0	0.0	0.0669917229057014	8.826030331406675E-4
38-39	0.0	0.0	0.0	0.12380122057541412	9.256568396353343E-4
40-41	0.0	0.0	0.0	0.18819895163981187	9.256568396353343E-4
42-43	0.0	0.0	0.0	0.25764474151570926	9.256568396353343E-4
44-45	0.0	0.0	0.0	0.3415027931156963	9.471837428826676E-4
46-47	0.0	0.0	0.0	0.4506549560313001	9.902375493773344E-4
48-49	0.0	0.0	0.0	0.5815708181299579	0.0010548182591193344
50-51	0.0	0.0	0.0	0.7258871775000808	0.0010548182591193344
52-53	0.0	0.0	0.0	0.8691810089659552	0.0011624527753560012
54-55	0.0	0.0	0.0	1.030449804643353	0.0011624527753560012
56-57	0.0	0.0	0.0	1.2285403683253144	0.0012270334850980013
58-59	0.0	0.0	0.0	1.4717620846653103	0.0013131410980873346
60-61	0.0	0.0	0.0	1.7278030718890933	0.0013561949045820013
62-63	0.0	0.0	0.0	1.9996340426447954	0.0015929908403026682
64-65	0.0	0.0	0.0	2.2792039351179136	0.0016145177435500015
66-67	0.0	0.0	0.0	2.604066432023421	0.0016145177435500015
68-69	0.0	0.0	0.0	2.9733066399733064	0.0016145177435500015
70-71	0.0	0.0	0.0	3.383878502158072	0.001636044646797335
72-73	0.0	0.0	0.0	3.812156242263769	0.0017113888081630017
74-75	0.0	0.0	0.0	4.229476788616573	0.0017974964211523352
76-77	0.0	0.0	0.0	4.711065904614292	0.001872840582518002
78-79	0.0	0.0	0.0	5.231995436296511	0.0018943674857653352
80-81	0.0	0.0	0.0	5.796280151118861	0.0019374212922600022
82-83	0.0	0.0	0.0	6.380326563122262	0.0020235289052493353
84-85	0.0	0.0	0.0	6.953200512340297	0.0020235289052493353
86-87	0.0	0.0	0.0	7.552950800262629	0.0021526903247333353
88	0.0	0.0	0.0	8.035648551777584	0.0022818517442173354
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5885	0.0	37.548412	94
GTACTAG	6090	0.0	37.099766	1
TAGGCAT	7410	0.0	33.03228	5
GCATCGT	6635	0.0	32.126694	8
CAACGCA	7625	0.0	31.546352	5
CTAGGCA	8200	0.0	30.710634	4
GTATCAA	9840	0.0	28.569597	1
TAGTACT	8410	0.0	28.43542	4
CTGTGCG	8390	0.0	28.094181	9
ATCAACG	8735	0.0	27.648764	3
CTAACGC	2700	0.0	27.32193	3
GTACTGT	8965	0.0	26.91756	6
CTAGTAC	9450	0.0	26.750132	3
TCTAACG	2865	0.0	26.240992	2
CGTTAAC	935	0.0	26.232801	1
TACTGTG	9435	0.0	26.125166	7
TAACGCC	2860	0.0	26.119745	4
GTACATG	19080	0.0	25.858675	1
ACTGTGC	9620	0.0	25.818542	8
CGCCTAC	2870	0.0	25.68381	7
>>END_MODULE