##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146416_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4645350
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76834684146512	31.0	31.0	34.0	30.0	34.0
2	31.758674373298028	31.0	31.0	34.0	30.0	34.0
3	31.951729579041405	31.0	31.0	34.0	30.0	34.0
4	35.690735681918476	37.0	35.0	37.0	33.0	37.0
5	35.59749190050265	37.0	35.0	37.0	33.0	37.0
6	35.632203816719944	37.0	35.0	37.0	33.0	37.0
7	35.55769489920028	37.0	35.0	37.0	33.0	37.0
8	35.53197261777907	37.0	35.0	37.0	33.0	37.0
9	37.13179523609631	39.0	37.0	39.0	33.0	39.0
10-11	37.084574466940055	39.0	37.0	39.0	33.0	39.0
12-13	37.01705016844802	39.0	37.0	39.0	33.0	39.0
14-15	38.22769931221544	40.0	37.0	41.0	33.0	41.0
16-17	38.203842767498685	40.0	37.0	41.0	33.0	41.0
18-19	38.22605616369057	40.0	37.0	41.0	33.0	41.0
20-21	38.230775829593036	40.0	37.0	41.0	33.0	41.0
22-23	38.18845501415394	40.0	37.0	41.0	33.0	41.0
24-25	38.121345861991024	40.0	37.0	41.0	33.0	41.0
26-27	37.98938831304423	40.0	37.0	41.0	33.0	41.0
28-29	37.91743485420905	40.0	37.0	41.0	33.0	41.0
30-31	37.81951090875822	40.0	37.0	41.0	32.0	41.0
32-33	37.746783988289366	40.0	37.0	41.0	32.0	41.0
34-35	37.669034841292905	40.0	36.5	41.0	32.0	41.0
36-37	37.59512221899318	40.0	36.0	41.0	32.0	41.0
38-39	37.48592990840302	40.0	36.0	41.0	31.0	41.0
40-41	37.37106493590365	39.5	36.0	41.0	31.0	41.0
42-43	37.252215118344154	39.0	36.0	41.0	31.0	41.0
44-45	37.08344075258054	39.0	35.0	41.0	31.0	41.0
46-47	36.89895422304025	39.0	35.0	41.0	31.0	41.0
48-49	36.6762465691498	39.0	35.0	41.0	30.0	41.0
50-51	36.450180825987275	38.5	35.0	40.5	30.0	41.0
52-53	36.22817107430011	38.0	35.0	40.0	30.0	41.0
54-55	35.973612106730386	38.0	34.5	40.0	29.0	41.0
56-57	35.68617359294779	37.0	34.0	40.0	29.0	41.0
58-59	35.377266729094686	36.5	34.0	40.0	29.0	41.0
60-61	35.08421270733099	36.0	34.0	39.0	28.0	41.0
62-63	34.7622737791555	35.5	34.0	39.0	28.0	41.0
64-65	34.44925656839635	35.0	33.0	38.5	28.0	40.5
66-67	34.1960570247667	35.0	33.0	37.5	28.0	40.0
68-69	33.97547935031806	35.0	33.0	37.0	28.0	40.0
70-71	33.755643600589835	35.0	33.0	36.5	27.5	39.0
72-73	33.54444229175412	35.0	33.0	36.0	27.0	39.0
74-75	33.37871355226194	35.0	33.0	36.0	27.0	39.0
76-77	33.25329027952684	35.0	33.0	35.0	27.0	37.5
78-79	33.17041977461332	35.0	33.0	35.0	28.0	37.0
80-81	33.055614969808516	35.0	33.0	35.0	27.5	36.5
82-83	32.911998988235545	35.0	33.0	35.0	27.0	36.0
84-85	32.82645258161387	35.0	33.0	35.0	27.0	36.0
86-87	32.73863454852702	35.0	33.0	35.0	27.0	36.0
88-89	32.66803201050513	35.0	33.0	35.0	27.0	35.5
90-91	32.60156888070867	35.0	33.0	35.0	27.0	35.0
92-93	32.54852013303626	35.0	33.0	35.0	27.0	35.0
94-95	32.52646840388776	35.0	33.0	35.0	27.0	35.0
96-97	32.48446263467768	35.0	33.0	35.0	27.0	35.0
98-99	32.45360188145135	35.0	33.0	35.0	27.0	35.0
100	31.920850958485367	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.523342855413155
1101	2	0.6240932670181536
1101	3	0.575668349279983
1101	4	0.3852146499019611
1101	5	0.4009317895815485
1101	6	0.439267491722525
1101	7	0.44982325189084094
1101	8	0.4459745689578014
1101	9	0.537262584197137
1101	10-11	0.5619734837799442
1101	12-13	0.5711754158133786
1101	14-15	0.7004951716255334
1101	16-17	0.6836245608128806
1101	18-19	0.7016483193595988
1101	20-21	0.6894157857214083
1101	22-23	0.7135588576218908
1101	24-25	0.7400775966606332
1101	26-27	0.743875017540816
1101	28-29	0.8264107071656568
1101	30-31	0.8088323660686996
1101	32-33	0.8285954910415683
1101	34-35	0.8215217392314642
1101	36-37	0.8046490585495008
1101	38-39	0.821539447605204
1101	40-41	0.8004439050743528
1101	42-43	0.7956534085269524
1101	44-45	0.7791821817623372
1101	46-47	0.8478396063081917
1101	48-49	0.870412808903346
1101	50-51	0.7742618380737696
1101	52-53	0.8106212089138367
1101	54-55	0.7809050440090743
1101	56-57	0.7405387422152572
1101	58-59	0.7243424304630892
1101	60-61	0.7851180373047697
1101	62-63	0.7305734716962249
1101	64-65	0.7267972500149114
1101	66-67	0.7354576125131445
1101	68-69	0.7018135417956799
1101	70-71	0.6350454549702036
1101	72-73	0.5758971512446749
1101	74-75	0.5953703589635566
1101	76-77	0.6333851795042946
1101	78-79	0.5913106986904282
1101	80-81	0.6024633618209521
1101	82-83	0.5472883405561504
1101	84-85	0.5788641388196396
1101	86-87	0.558295668166032
1101	88-89	0.5504720732346655
1101	90-91	0.6390495856611125
1101	92-93	0.6077320148285352
1101	94-95	0.5562333736113558
1101	96-97	0.6467520822907389
1101	98-99	0.5747930964615975
1101	100	0.5936362961505921
1102	1	0.454667242784879
1102	2	0.5689210311518238
1102	3	0.4559834810974941
1102	4	0.3451892013088127
1102	5	0.37411858241841145
1102	6	0.3682066088492775
1102	7	0.39510592135403755
1102	8	0.40159993580871145
1102	9	0.4773474427532989
1102	10-11	0.4798382411698938
1102	12-13	0.530322296494596
1102	14-15	0.6164028469522265
1102	16-17	0.6434989229154837
1102	18-19	0.6977064693337596
1102	20-21	0.7003696633585008
1102	22-23	0.6258856703395708
1102	24-25	0.6447781423022931
1102	26-27	0.6730454113515663
1102	28-29	0.7015641268178072
1102	30-31	0.726118217931699
1102	32-33	0.7070982926710343
1102	34-35	0.6961808916493268
1102	36-37	0.7205533177523407
1102	38-39	0.7419346859976201
1102	40-41	0.7685069200167831
1102	42-43	0.8020944335843723
1102	44-45	0.7876754304818476
1102	46-47	0.7890895670482649
1102	48-49	0.7490298212409598
1102	50-51	0.6679581819850497
1102	52-53	0.71270964348885
1102	54-55	0.7816733149690975
1102	56-57	0.783304024117875
1102	58-59	0.7895404290020096
1102	60-61	0.7571416654608072
1102	62-63	0.681759509110833
1102	64-65	0.6980838112079866
1102	66-67	0.7218925415740216
1102	68-69	0.6999629714151681
1102	70-71	0.6902913159537434
1102	72-73	0.6843990575437573
1102	74-75	0.6376741072366272
1102	76-77	0.5975233740532602
1102	78-79	0.5475924711916704
1102	80-81	0.5499988375024358
1102	82-83	0.5651146436085526
1102	84-85	0.6269362416712738
1102	86-87	0.5681747414603464
1102	88-89	0.5435962316954956
1102	90-91	0.5465024054215277
1102	92-93	0.5309476458692117
1102	94-95	0.5302051611190706
1102	96-97	0.5064530990515692
1102	98-99	0.5933325536099474
1102	100	0.6698551181568746
1103	1	0.32270192120649455
1103	2	-0.1559729059305397
1103	3	0.19766829283219423
1103	4	0.21081085890201479
1103	5	0.27015004092999817
1103	6	0.30501220324502754
1103	7	0.33578016843141256
1103	8	0.348468425775998
1103	9	0.37565982877247706
1103	10-11	0.46290155295042723
1103	12-13	0.4757286074627771
1103	14-15	0.5373441908522096
1103	16-17	0.602315023670208
1103	18-19	0.5942673077311511
1103	20-21	0.5448604244897552
1103	22-23	0.5488652151948514
1103	24-25	0.5489325872070125
1103	26-27	0.566840452797031
1103	28-29	0.6200946661067306
1103	30-31	0.698971594041069
1103	32-33	0.6442866582540461
1103	34-35	0.6197800215116374
1103	36-37	0.6673654379776934
1103	38-39	0.6445275689353593
1103	40-41	0.7164523478132097
1103	42-43	0.6534472549457888
1103	44-45	0.6651670720435092
1103	46-47	0.6423901473960711
1103	48-49	0.6864149564723405
1103	50-51	0.7514535698675786
1103	52-53	0.7326630518331854
1103	54-55	0.6771665963962477
1103	56-57	0.6403392013744877
1103	58-59	0.5747996264477848
1103	60-61	0.6714154433497441
1103	62-63	0.6724452636007214
1103	64-65	0.688296834695862
1103	66-67	0.6524605161235968
1103	68-69	0.5630469248072032
1103	70-71	0.5157864721992667
1103	72-73	0.5077395937526461
1103	74-75	0.5338703102100624
1103	76-77	0.5596224624503208
1103	78-79	0.4863092146934065
1103	80-81	0.45997556451347776
1103	82-83	0.4797418320343638
1103	84-85	0.4195777436806054
1103	86-87	0.48718730802173127
1103	88-89	0.47066461296324036
1103	90-91	0.49932524543942236
1103	92-93	0.5188450098326243
1103	94-95	0.5271161686239907
1103	96-97	0.5056661980938486
1103	98-99	0.49971882242804355
1103	100	0.5124880716576214
1104	1	0.38486031869039294
1104	2	0.482847042270258
1104	3	0.3840150324227132
1104	4	0.2569525792694449
1104	5	0.3124049795878747
1104	6	0.2981196177644563
1104	7	0.3023988093455827
1104	8	0.3500813156369986
1104	9	0.368506194478897
1104	10-11	0.3542800471998717
1104	12-13	0.4287386532130739
1104	14-15	0.4834476191728072
1104	16-17	0.48491418939837416
1104	18-19	0.5091528545950794
1104	20-21	0.5225291400937948
1104	22-23	0.520146171541569
1104	24-25	0.4656677938925071
1104	26-27	0.550525072481598
1104	28-29	0.5552375394809559
1104	30-31	0.5953504151343338
1104	32-33	0.535729191386757
1104	34-35	0.5893028837510101
1104	36-37	0.5648437807809685
1104	38-39	0.5447932740367065
1104	40-41	0.5100938749216581
1104	42-43	0.5008102271656298
1104	44-45	0.5711901472513787
1104	46-47	0.6011642638069645
1104	48-49	0.5437878318724145
1104	50-51	0.6193624271919163
1104	52-53	0.5430628181424098
1104	54-55	0.5605915670527679
1104	56-57	0.5542592631846261
1104	58-59	0.5766081366834186
1104	60-61	0.5932206933310482
1104	62-63	0.5898933035317881
1104	64-65	0.5695569978422483
1104	66-67	0.5901876380535214
1104	68-69	0.584444206523699
1104	70-71	0.5468019815779428
1104	72-73	0.5405176898493664
1104	74-75	0.5002898099749942
1104	76-77	0.4873701746884933
1104	78-79	0.42312375696219107
1104	80-81	0.47361426954633146
1104	82-83	0.45170540093428
1104	84-85	0.4505555134245327
1104	86-87	0.43089701880910525
1104	88-89	0.45101121438253955
1104	90-91	0.4372121832565412
1104	92-93	0.5101177768405876
1104	94-95	0.4844705944831773
1104	96-97	0.4500628350226705
1104	98-99	0.4290168774975953
1104	100	0.5474789447527755
1105	1	0.14285620510448283
1105	2	-0.40176517751592655
1105	3	0.03338643718738865
1105	4	0.09533062639575007
1105	5	0.18880663692561228
1105	6	0.20193527319765536
1105	7	0.24495611857319233
1105	8	0.1890341159758293
1105	9	0.29872340344130777
1105	10-11	0.31805388163772363
1105	12-13	0.29544763915601635
1105	14-15	0.36297195812009875
1105	16-17	0.36919619425938066
1105	18-19	0.3581842691315913
1105	20-21	0.35975487796480365
1105	22-23	0.36810128196616176
1105	24-25	0.36463380905685483
1105	26-27	0.3735967425808653
1105	28-29	0.39010457459868775
1105	30-31	0.43804556478470147
1105	32-33	0.4906415147046488
1105	34-35	0.5212740934940641
1105	36-37	0.44744145768454047
1105	38-39	0.41529743053014556
1105	40-41	0.4237224293256361
1105	42-43	0.3879133325631159
1105	44-45	0.45556609882955
1105	46-47	0.42134130232961553
1105	48-49	0.48671660261336314
1105	50-51	0.39469227878724666
1105	52-53	0.39329332497716507
1105	54-55	0.3805032017740899
1105	56-57	0.43528541739703996
1105	58-59	0.39036920267242436
1105	60-61	0.3177852165592441
1105	62-63	0.3141156565527581
1105	64-65	0.38821080194033897
1105	66-67	0.41450078893299036
1105	68-69	0.4421765057856817
1105	70-71	0.3296083828269687
1105	72-73	0.2990464081323978
1105	74-75	0.33873024602617363
1105	76-77	0.3392652339562474
1105	78-79	0.2597652604418528
1105	80-81	0.32032298558710437
1105	82-83	0.33244412663616885
1105	84-85	0.30867455135478394
1105	86-87	0.2867178570560398
1105	88-89	0.33977418352787225
1105	90-91	0.27443890730855713
1105	92-93	0.2813682301378222
1105	94-95	0.3163519060416391
1105	96-97	0.2825503537774168
1105	98-99	0.26397917424049666
1105	100	0.2685390305124571
1106	1	0.2763849768646409
1106	2	0.341598524787571
1106	3	0.3113246075759939
1106	4	0.19514104929007203
1106	5	0.21255982401898166
1106	6	0.20949582797170052
1106	7	0.24477643244694036
1106	8	0.20529323765685348
1106	9	0.32176224202052595
1106	10-11	0.3067449204103596
1106	12-13	0.3133187157341766
1106	14-15	0.39682685745191293
1106	16-17	0.3417303181383957
1106	18-19	0.39802403526488916
1106	20-21	0.39313446078526937
1106	22-23	0.37718037683164596
1106	24-25	0.36171322263167127
1106	26-27	0.43101635089807644
1106	28-29	0.37804197568889464
1106	30-31	0.36493501660464744
1106	32-33	0.40561480906019654
1106	34-35	0.3191321469493076
1106	36-37	0.3957242737562865
1106	38-39	0.4082366449665784
1106	40-41	0.432267411302071
1106	42-43	0.34735454717539227
1106	44-45	0.3685276778694657
1106	46-47	0.3878460499997445
1106	48-49	0.292448425191175
1106	50-51	0.30666338613733757
1106	52-53	0.2937749607253579
1106	54-55	0.28340483048869913
1106	56-57	0.3075596283166817
1106	58-59	0.25360250889715275
1106	60-61	0.2477219142909206
1106	62-63	0.24539120824960747
1106	64-65	0.27022845184362154
1106	66-67	0.29836557924422635
1106	68-69	0.23834375125781548
1106	70-71	0.2519224324137497
1106	72-73	0.27283366415490207
1106	74-75	0.27796364017071795
1106	76-77	0.24622930772097362
1106	78-79	0.18903459181164095
1106	80-81	0.17565916898784195
1106	82-83	0.21314682577455812
1106	84-85	0.23506011272282024
1106	86-87	0.29570738360782656
1106	88-89	0.2206790982456468
1106	90-91	0.2555932945074346
1106	92-93	0.26965676099767677
1106	94-95	0.3250806953857577
1106	96-97	0.27522498451418187
1106	98-99	0.26333543632947
1106	100	0.3361878348029492
1107	1	0.1454968701556787
1107	2	-0.42984189722234234
1107	3	-0.00872921140560834
1107	4	0.07093599036701193
1107	5	0.1558983861167107
1107	6	0.22847656576135478
1107	7	0.2155874304665133
1107	8	0.1910627637424156
1107	9	0.2013355292799801
1107	10-11	0.2591577888835346
1107	12-13	0.24491511145721745
1107	14-15	0.286260261945408
1107	16-17	0.3891337231487668
1107	18-19	0.3490325928468039
1107	20-21	0.27853683137760754
1107	22-23	0.2912218960319706
1107	24-25	0.26329259738344035
1107	26-27	0.30933707251517717
1107	28-29	0.27916871604112004
1107	30-31	0.314043868189799
1107	32-33	0.3101000925764197
1107	34-35	0.3395523648231844
1107	36-37	0.3350435335183093
1107	38-39	0.4089325733312563
1107	40-41	0.437785914191096
1107	42-43	0.3602341483293685
1107	44-45	0.32260567203700674
1107	46-47	0.3913806006501801
1107	48-49	0.44190566176481383
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2216	76-77	-1.318307694142316
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2216	80-81	-1.1743825204337668
2216	82-83	-1.1140090839449783
2216	84-85	-1.0906725031996771
2216	86-87	-0.9578852518708203
2216	88-89	-1.0719404928726526
2216	90-91	-1.0847964128771466
2216	92-93	-1.1488326540268687
2216	94-95	-1.1446094191812684
2216	96-97	-1.0652020782128346
2216	98-99	-1.0922838170253293
2216	100	-1.1138275861699682
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	24.0
14	159.0
15	435.0
16	1167.0
17	2414.0
18	4031.0
19	6735.0
20	9884.0
21	14545.0
22	20453.0
23	25715.0
24	31176.0
25	37661.0
26	47835.0
27	61441.0
28	77696.0
29	96743.0
30	119583.0
31	147030.0
32	182822.0
33	229156.0
34	309239.0
35	433096.0
36	679397.0
37	1138752.0
38	806574.0
39	161436.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36804430322788	20.231755727688956	13.114402209133042	25.285797759950118
2	15.870949547485985	19.780050633347397	38.11420919839145	26.234790620775172
3	19.369832787079343	24.663706669680483	28.507031338295363	27.459429204944808
4	13.547200997046154	16.29493479709172	36.49140697975374	33.666457226108385
5	13.98025802387783	37.94676215101559	33.812369637750905	14.26061018735567
6	32.11930209779672	36.645161290322584	16.835889653093954	14.399646958786743
7	28.006192015778403	30.913774393547765	22.389269067023555	18.690764523650273
8	27.227377915549962	34.32669228368153	18.753054129398215	19.692875671370295
9	26.166316854488898	14.529497239174658	20.410840948475357	38.89334495786109
10-11	27.144208724853886	24.77243910577244	25.92459125792459	22.158760911449082
12-13	27.064279333096536	22.141711604077198	26.52852852852853	24.26548053429774
14-15	24.350308734004358	23.33772234155167	23.861130947524153	28.450837976919818
16-17	23.788001429426377	26.10592178626631	24.963812262928347	25.142264521378966
18-19	23.188435747575532	26.464819658368043	26.79446112779446	23.55228346626196
20-21	24.82919196662725	25.78382473729355	26.01221458937455	23.374768706704657
22-23	25.242508087190757	25.02930375568127	26.004222754611156	23.723965402516818
24-25	24.761637930756116	25.019196620103244	26.455288935233927	23.763876513906705
26-27	24.558156906622063	25.37621284114142	25.379818603256922	24.6858116489796
28-29	23.733529228152882	25.757746994306135	26.363244965395506	24.14547881214548
30-31	24.437458964340685	25.54297308060749	26.241456510273714	23.778111444778112
32-33	24.66267342611429	25.55283240229477	25.418482999128162	24.366011172462784
34-35	24.613526174867083	25.6714156613927	26.063508027625137	23.65155013611508
36-37	24.462440935559215	24.90119151409474	25.464938056335907	25.171429494010138
38-39	24.546040664320234	25.165703337746347	26.058499359574625	24.229756638358786
40-41	24.162425041662804	24.29916450508742	26.93137764901765	24.60703280423212
42-43	24.301869570809746	24.48662551052754	26.196433290081895	25.015071628580827
44-45	24.705253640737514	24.846610050911124	26.088798475895246	24.359337832456113
46-47	24.507087732894185	25.15314238970153	25.298158373427192	25.041611503977094
48-49	25.29899155570113	25.05376643844072	25.469743926337962	24.177498079520188
50-51	24.527075462559335	24.772632847901665	25.89721980044561	24.803071889093395
52-53	24.43940714908457	24.97243480039179	25.532015886854598	25.056142163669044
54-55	24.165847833647145	24.963799669366903	25.493406766213944	25.37694573077201
56-57	25.233919579518986	24.6566644705621	25.039240883322698	25.070175066596217
58-59	23.852920592912337	25.250880398231264	25.91812958402838	24.978069424828018
60-61	24.116069705456848	25.423571740019824	25.46054422420373	24.9998143303196
62-63	24.647163292324585	24.96737597812867	24.86946085870817	25.51599987083858
64-65	23.807947732678915	25.105761675654147	25.565727017339924	25.520563574327014
66-67	24.45576759555254	25.09884077626013	25.36561292475271	25.07977870343462
68-69	24.034384100959112	24.68305238008463	25.922114814662134	25.360448704294125
70-71	23.630078118567102	24.640947036801954	25.72924888443948	25.999725960191466
72-73	24.224654762289173	24.601859924440568	25.98902127934386	25.184464033926403
74-75	24.331841013110132	24.907041229929614	25.75947632670081	25.001641430259447
76-77	24.132678680123547	24.9130851528161	24.923590603864305	26.030645563196046
78-79	24.272131199417565	25.029139508092594	24.862229783467274	25.836499509022566
80-81	23.739192986637036	25.61819060341887	25.524222424079788	25.1183939858643
82-83	24.583389887391675	24.64151364600543	26.45784881170819	24.317247654894704
84-85	24.44179264878757	24.972817958306553	25.452735324469238	25.13265406843664
86-87	23.200887757053902	25.553973555664417	25.891123602774574	25.354015084507104
88-89	24.104878984751792	24.320746785906696	25.73114264939136	25.84323157995015
90-91	23.654494383534292	24.70982266596843	25.39196854284052	26.24371440765676
92-93	24.04338203135319	24.804407224798457	25.597671950541223	25.554538793307135
94-95	23.21254162030229	24.658044561036185	26.289500134437855	25.83991368422367
96-97	23.446721542195316	24.563302314031386	25.821763212284036	26.16821293148926
98-99	22.930924711923936	24.968680422990545	25.75526760357203	26.34512726151349
100	23.56965931132339	24.28885577269238	25.160069795364453	26.981415120619772
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	27.0
2	34.5
3	55.5
4	47.0
5	35.5
6	72.5
7	168.0
8	279.0
9	331.0
10	283.0
11	218.0
12	213.5
13	228.5
14	258.0
15	328.0
16	435.0
17	572.0
18	757.0
19	952.5
20	1172.0
21	1453.5
22	1708.5
23	2029.5
24	2448.0
25	2839.5
26	3339.5
27	3975.5
28	4892.5
29	6798.0
30	9697.5
31	12851.0
32	18192.0
33	24851.5
34	30951.0
35	38052.0
36	47194.0
37	63791.0
38	79922.0
39	91437.0
40	96517.5
41	99335.0
42	107424.5
43	120910.5
44	141685.5
45	174525.0
46	209270.0
47	236135.0
48	255347.5
49	257351.0
50	248589.5
51	255785.5
52	262038.5
53	254277.5
54	246423.5
55	217051.5
56	191353.5
57	163673.0
58	133434.5
59	128266.5
60	108103.5
61	77393.5
62	58446.0
63	47186.0
64	34496.5
65	19002.5
66	11920.0
67	8947.0
68	7003.5
69	5324.0
70	3897.0
71	2864.5
72	2059.0
73	1729.0
74	1447.5
75	1164.0
76	872.0
77	547.0
78	309.0
79	158.5
80	98.0
81	40.0
82	19.0
83	10.0
84	4.5
85	4.0
86	2.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013605002852314679
2	0.004090111616993337
3	0.0016145177435500017
4	0.026521144800714696
5	0.002604755292927336
6	0.0
7	2.798497422153336E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008890611041148674
16-17	0.002798497422153336
18-19	0.0
20-21	7.534416136566673E-5
22-23	8.826030331406675E-4
24-25	2.1526903247333357E-5
26-27	1.6145177435500016E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.444304519573337E-4
36-37	0.0
38-39	0.0
40-41	4.1977461332300044E-4
42-43	6.99624355538334E-4
44-45	0.0
46-47	0.0
48-49	5.704629360543339E-4
50-51	0.0
52-53	0.0
54-55	4.1977461332300044E-4
56-57	7.534416136566673E-5
58-59	1.9374212922600018E-4
60-61	7.534416136566673E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.001366958356205668
70-71	8.503126782696674E-4
72-73	0.0
74-75	2.3679593572066692E-4
76-77	0.003067583712745003
78-79	5.274091295596672E-4
80-81	0.003455067971197004
82-83	0.001926657840636335
84-85	0.006016769457629673
86-87	0.008298621201847007
88-89	0.008126405975868342
90-91	0.0065118882323183394
92-93	0.009288858751224342
94-95	0.0017436791630340016
96-97	0.004983478101757671
98-99	0.0019912385503783356
100	0.08139322117816741
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4645350.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.797768617305433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.31710102938817	15.54401257050542
2	15.894646559936062	7.565142421744163
3	6.757247068169255	4.824222066547723
4	3.5371117753642043	3.36701470414655
5	1.980095370359062	2.3560925732001325
6	1.3292843753944916	1.8980401195342493
7	0.8895660558732464	1.481878101723636
8	0.638526694742352	1.2156408429961048
9	0.47029910711378153	1.0072862398817204
>10	2.5404459869051066	11.097541915259491
>50	0.2610324926876121	4.367002886192565
>100	0.28466485581798756	14.983088263329078
>500	0.0550108651363054	9.05525815397616
>1k	0.04288675999064059	17.497655560886336
>5k	0.0019000463286992662	2.8795820837130974
>10k+	1.8095679320945395E-4	0.8605414963635644
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	22547	0.4853670875176252	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	17419	0.3749771276652997	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	8814	0.1897381252219962	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8632	0.18582022883098154	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7902	0.17010558946042817	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7705	0.16586478952070352	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7442	0.16020321396665482	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7230	0.15563951047822014	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6825	0.14692111466305016	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6614	0.1423789380778628	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6582	0.14169007717394813	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6474	0.13936517162323614	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6051	0.13025929154961413	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5890	0.12679346012679346	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	5485	0.11807506431162344	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5469	0.11773063385966613	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5414	0.11654665418106278	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5350	0.11516893237323346	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5316	0.11443701766282412	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	5216	0.11228432733809078	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5187	0.1116600471439181	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	5103	0.10985178727114212	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	5035	0.10838795785032346	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	4948	0.10651511726780544	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4861	0.10464227668528743	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	4771	0.10270485539302743	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	4739	0.10201599448911276	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4699	0.10115491835921944	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	4685	0.10085354171375677	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1526903247333353E-5
2	0.0	0.0	0.0	0.0	2.1526903247333353E-5
3	0.0	0.0	0.0	0.0	2.1526903247333353E-5
4	0.0	0.0	0.0	0.0	2.1526903247333353E-5
5	0.0	0.0	0.0	0.0	2.1526903247333353E-5
6	0.0	0.0	0.0	0.0	4.305380649466671E-5
7	0.0	0.0	0.0	0.0	4.305380649466671E-5
8	0.0	0.0	0.0	0.0	4.305380649466671E-5
9	0.0	0.0	0.0	0.0	4.305380649466671E-5
10-11	0.0	0.0	0.0	0.0	5.381725811833339E-5
12-13	0.0	0.0	0.0	0.0	2.7984974221533366E-4
14-15	0.0	0.0	0.0	0.0	3.229035487100003E-4
16-17	0.0	0.0	0.0	1.0763451623666677E-5	4.197746133230004E-4
18-19	0.0	0.0	0.0	2.1526903247333353E-5	4.305380649466671E-4
20-21	0.0	0.0	0.0	2.1526903247333353E-5	4.951187746886672E-4
22-23	0.0	0.0	0.0	3.229035487100003E-5	5.381725811833338E-4
24-25	0.0	0.0	0.0	1.1839796786033345E-4	5.596994844306672E-4
26-27	0.0	0.0	0.0	3.121400970863337E-4	6.242801941726673E-4
28-29	0.0	0.0	0.0	0.0015929908403026685	6.242801941726673E-4
30-31	0.0	0.0	0.0	0.0066841034582970065	6.35043645796334E-4
32-33	0.0	0.0	0.0	0.01768435101768435	6.458070974200006E-4
34-35	0.0	0.0	0.0	0.03437846448599137	6.888609039146673E-4
36-37	0.0	0.0	0.0	0.06819722948755207	6.888609039146673E-4
38-39	0.0	0.0	0.0	0.1256848246095558	7.103878071620007E-4
40-41	0.0	0.0	0.0	0.1913849333204172	7.103878071620007E-4
42-43	0.0	0.0	0.0	0.26197164906842324	7.319147104093341E-4
44-45	0.0	0.0	0.0	0.34747650876683134	7.642050652803341E-4
46-47	0.0	0.0	0.0	0.4584692219100821	7.857319685276674E-4
48-49	0.0	0.0	0.0	0.5921943448825169	8.395492266460009E-4
50-51	0.0	0.0	0.0	0.7389862981260831	9.041299363880009E-4
52-53	0.0	0.0	0.0	0.8845512178845512	0.0010117644526246676
54-55	0.0	0.0	0.0	1.046950175982434	0.0010440548074956678
56-57	0.0	0.0	0.0	1.2476993122154414	0.0010763451623666677
58-59	0.0	0.0	0.0	1.494020902623053	0.0010978720656140012
60-61	0.0	0.0	0.0	1.7520100745907197	0.0010978720656140012
62-63	0.0	0.0	0.0	2.0263704564779834	0.001270087291592668
64-65	0.0	0.0	0.0	2.3093308362125566	0.0012916141948400013
66-67	0.0	0.0	0.0	2.638595584832144	0.0013346680013346678
68-69	0.0	0.0	0.0	3.011043301365882	0.0013561949045820013
70-71	0.0	0.0	0.0	3.426900018297868	0.0013777218078293348
72-73	0.0	0.0	0.0	3.8605379573121508	0.0014745928724423348
74-75	0.0	0.0	0.0	4.284273520832661	0.0015499370338080015
76-77	0.0	0.0	0.0	4.776174023485852	0.0015499370338080015
78-79	0.0	0.0	0.0	5.309352363115805	0.0015607004854316683
80-81	0.0	0.0	0.0	5.885433820917692	0.0015929908403026682
82-83	0.0	0.0	0.0	6.48121239519089	0.001636044646797335
84-85	0.0	0.0	0.0	7.066507367582636	0.0016575715500446683
86-87	0.0	0.0	0.0	7.678840130453034	0.001700625356539335
88	0.0	0.0	0.0	8.171504838171504	0.0017436791630340018
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5810	0.0	36.754967	94
TACCCCG	370	0.0	35.56541	5
GTACTAG	5735	0.0	34.68004	1
TAGGCAT	7000	0.0	30.615288	5
GTATCAA	12335	0.0	29.351086	1
CAACGCA	11350	0.0	28.612364	5
CTAGGCA	8080	0.0	27.811153	4
GGTATCA	8565	0.0	27.338495	1
ATCAACG	12040	0.0	27.058844	3
TAGTACT	8505	0.0	26.863613	4
CTGTGCG	8690	0.0	26.665524	9
GCATCGT	7210	0.0	25.877209	8
GTACTGT	9215	0.0	25.244806	6
CTAACGC	2735	0.0	24.923735	3
TACTGTG	10080	0.0	24.850143	7
ACTGTGC	10020	0.0	24.436117	8
CGTTAAC	995	0.0	24.100147	1
TATCAAC	15085	0.0	23.78014	2
GTACATG	18610	0.0	23.74948	1
AACGCAG	14685	0.0	23.714088	6
>>END_MODULE