##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR873436.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72610535 Sequences flagged as poor quality 0 Sequence length 36 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.774506371561646 31.0 31.0 34.0 30.0 34.0 2 32.146786041447015 33.0 31.0 34.0 30.0 34.0 3 32.299252443188855 34.0 31.0 34.0 30.0 34.0 4 35.79457708168656 37.0 35.0 37.0 35.0 37.0 5 35.66194510204339 37.0 35.0 37.0 33.0 37.0 6 35.66124247672875 37.0 35.0 37.0 33.0 37.0 7 35.66230319057696 37.0 35.0 37.0 33.0 37.0 8 35.7016136845707 37.0 35.0 37.0 35.0 37.0 9 37.40736281587789 39.0 37.0 39.0 35.0 39.0 10 37.297889321432486 39.0 37.0 39.0 34.0 39.0 11 37.36001953435545 39.0 37.0 39.0 34.0 39.0 12 37.30249398382755 39.0 37.0 39.0 34.0 39.0 13 37.184418418071154 39.0 37.0 39.0 34.0 39.0 14 38.44843598797337 40.0 38.0 41.0 34.0 41.0 15 38.44470221848661 40.0 38.0 41.0 34.0 41.0 16 38.38716786758285 40.0 38.0 41.0 34.0 41.0 17 38.357974596936934 40.0 38.0 41.0 33.0 41.0 18 38.32158878873431 40.0 38.0 41.0 33.0 41.0 19 38.29582460726945 40.0 38.0 41.0 34.0 41.0 20 38.17761138931148 40.0 38.0 41.0 33.0 41.0 21 38.15746031894683 40.0 38.0 41.0 33.0 41.0 22 38.11513786532491 40.0 38.0 41.0 33.0 41.0 23 38.1073880119462 40.0 38.0 41.0 33.0 41.0 24 38.09970934658449 40.0 38.0 41.0 33.0 41.0 25 38.101085482981226 40.0 38.0 41.0 33.0 41.0 26 37.98944226206294 40.0 38.0 41.0 33.0 41.0 27 37.912447732274664 40.0 38.0 41.0 33.0 41.0 28 37.81531109776288 40.0 37.0 41.0 33.0 41.0 29 37.70513368342486 40.0 37.0 41.0 33.0 41.0 30 37.56884957534055 40.0 37.0 41.0 32.0 41.0 31 37.48657224464742 40.0 37.0 41.0 32.0 41.0 32 37.32406341586658 40.0 37.0 41.0 32.0 41.0 33 37.202243985669575 39.0 37.0 41.0 31.0 41.0 34 37.050168202176174 39.0 36.0 41.0 31.0 41.0 35 36.861477236051215 39.0 36.0 41.0 31.0 41.0 36 36.67656660290411 39.0 36.0 40.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.023665331272990642 1101 2 0.039581788303330256 1101 3 0.03348979773851113 1101 4 0.01396521107464821 1101 5 0.010984306781736564 1101 6 -0.002497412135852528 1101 7 0.006531895914022812 1101 8 -0.023493160745104547 1101 9 0.016572291512474635 1101 10 -0.005813810534775143 1101 11 -0.018748431026750723 1101 12 -0.012251902692739236 1101 13 0.021147827994262514 1101 14 0.03694055920716721 1101 15 -0.016474411794916932 1101 16 -0.006250068004817422 1101 17 0.0056565327967987855 1101 18 0.012415429671449374 1101 19 -0.008488357372691269 1101 20 0.022646449551551484 1101 21 -0.022332975825449353 1101 22 -0.023060103452756664 1101 23 -0.038233486340701006 1101 24 -0.0695636555845951 1101 25 -0.04770305929863383 1101 26 -0.028998280814022337 1101 27 -0.08927001039526061 1101 28 -0.12118978229263178 1101 29 -0.20247122867379375 1101 30 -0.16942518753126734 1101 31 -0.2717682822491483 1101 32 -0.27355965035640395 1101 33 -0.2579873554729275 1101 34 -0.23717493500339515 1101 35 -0.30864415450921 1101 36 -0.3051639115179725 1102 1 -0.1410166216830291 1102 2 -0.04730203702497704 1102 3 -0.06728400405153678 1102 4 -0.054271521087336794 1102 5 -0.06736372070394481 1102 6 -0.07852459337843953 1102 7 -0.07420110539136004 1102 8 -0.06484130772025054 1102 9 -0.07658503478344869 1102 10 -0.11568179407653645 1102 11 -0.07654529820257494 1102 12 -0.0863707954856423 1102 13 -0.05765808715720766 1102 14 -0.03994938410117754 1102 15 -0.13534099567514346 1102 16 -0.13038413684117245 1102 17 -0.12260233152452571 1102 18 -0.1726615105073961 1102 19 -0.16111759961112426 1102 20 -0.17722558187902138 1102 21 -0.21800299731808082 1102 22 -0.20035641581033303 1102 23 -0.21695586142406853 1102 24 -0.215470098764051 1102 25 -0.21856875120806052 1102 26 -0.27928685957019184 1102 27 -0.29582219574746915 1102 28 -0.2446084471654686 1102 29 -0.32199674206086826 1102 30 -0.33017628371877805 1102 31 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27 -0.41839104624180123 1107 28 -0.49884017260136204 1107 29 -0.46948855363930875 1107 30 -0.5317088621895536 1107 31 -0.5010688939107837 1107 32 -0.6379770480464018 1107 33 -0.71256370956052 1107 34 -0.7766906615613962 1107 35 -0.7599109428383031 1107 36 -0.8030663443894923 1108 1 -0.6647539352074858 1108 2 -0.5193812668613695 1108 3 -0.4504715417908187 1108 4 -0.40632717313557976 1108 5 -0.39363003103407124 1108 6 -0.36774945602882525 1108 7 -0.31387853780443464 1108 8 -0.30678299677619947 1108 9 -0.4210762847365146 1108 10 -0.5875009196327312 1108 11 -0.46458550178645197 1108 12 -0.46201193198291435 1108 13 -0.4800411171934016 1108 14 -0.8089860435388871 1108 15 -0.8376169778600229 1108 16 -0.8024420225140005 1108 17 -0.8819435687836545 1108 18 -0.7802471628736782 1108 19 -0.6953865114943767 1108 20 -0.7621233634867721 1108 21 -0.7605652634637181 1108 22 -1.026338978174664 1108 23 -1.0415355583052275 1108 24 -1.1346814633722317 1108 25 -0.9305759087210959 1108 26 -1.085809788232062 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0.20499038163142558 2207 30 0.21684221709033125 2207 31 0.21349602713707583 2207 32 0.24531780562480066 2207 33 0.29754092298921364 2207 34 0.3038936350261636 2207 35 0.328138232397329 2207 36 0.3537524336582365 2208 1 -0.5186680901250149 2208 2 -0.6387991722567499 2208 3 -0.3950081040245941 2208 4 -0.1487958508288898 2208 5 -0.08379954304411541 2208 6 -0.039879341124390066 2208 7 -0.1207756787074743 2208 8 -0.11670507829161636 2208 9 -0.29375716789694195 2208 10 -0.08528261195986175 2208 11 -0.11254489010740798 2208 12 -0.190183114132914 2208 13 -0.12132914830022656 2208 14 -0.15384507295597416 2208 15 -0.24126715209261818 2208 16 -0.39802089316278 2208 17 -0.3339044500178332 2208 18 -0.2525292888837498 2208 19 -0.14869825517418178 2208 20 -0.3021446824171292 2208 21 -0.28057137723543235 2208 22 -0.14964333717121292 2208 23 -0.11085527302372356 2208 24 -0.13141918086002846 2208 25 -0.19297126348224225 2208 26 -0.16885687153368423 2208 27 -0.22816232884794374 2208 28 -0.26289184429083434 2208 29 -0.2414605085603938 2208 30 -0.3589532786619358 2208 31 -0.21129635185612017 2208 32 -0.3402339194440316 2208 33 -0.24471367762161833 2208 34 -0.20232502698405597 2208 35 -0.19218535951078763 2208 36 -0.2518483978757544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 224874.0 3 2454.0 4 375.0 5 698.0 6 1009.0 7 1008.0 8 1477.0 9 1845.0 10 2160.0 11 2814.0 12 3691.0 13 4641.0 14 6009.0 15 7429.0 16 9569.0 17 12915.0 18 17358.0 19 24185.0 20 33523.0 21 44638.0 22 57262.0 23 76974.0 24 107449.0 25 156824.0 26 235693.0 27 355377.0 28 530735.0 29 768344.0 30 1076760.0 31 1451029.0 32 1904724.0 33 2483084.0 34 3300428.0 35 4383464.0 36 6120173.0 37 9409296.0 38 1.9107693E7 39 2.0682554E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 62.94377562140576 16.91545264842652 3.223593860122733 16.91717787004498 2 33.396175762834936 19.00145244437782 30.900710407592502 16.70166138519474 3 31.370090882160483 22.046874490198938 20.839497543376055 25.743537084264528 4 30.538342679887982 33.41391438583947 17.338811785369714 18.708931148902842 5 31.774328614986793 31.9103612168675 17.6983009421429 18.61700922600281 6 25.895395206769926 31.738092826337116 15.783946503079752 26.582565463813207 7 23.28689356165741 15.162657870514245 36.298290599291136 25.252157968537208 8 24.352203161703738 17.413331274862525 23.434471320174133 34.799994243259604 9 25.74728722216411 17.39134273008731 24.406229757155764 32.45514029059282 10 27.481519038525192 33.98094505156862 18.367667997488244 20.169867912417942 11 32.68088576953744 21.45213914206802 16.87434888064108 28.992626207753464 12 28.24270885760586 18.46151388362584 24.048679712936973 29.24709754583133 13 25.5867705698629 22.616287016753148 26.410407250132508 25.38653516325145 14 25.724291936424926 22.86682228687614 25.327001102525966 26.08188467417297 15 27.238248554428086 21.57613905475287 24.248704406323405 26.93690798449564 16 28.74143263095362 22.49093881487032 22.9234325294532 25.84419602472286 17 27.58228265361218 22.7926484772492 22.880995161377065 26.74407370776155 18 27.459987727676154 22.75887514118991 22.75961194887216 27.021525182261776 19 28.5752942048974 23.138273254700575 22.690018741770736 25.596413798631286 20 27.227501881043146 24.00625886877828 23.05153788292546 25.714701367253113 21 28.02928362665118 22.80062709507421 22.862667253013143 26.307422025261467 22 27.51717967077227 24.30032531094319 22.66103690470239 25.521458113582145 23 26.979293018568175 23.525352071844672 23.787720335623476 25.707634573963684 24 26.91779230107587 23.324733800680576 22.948455069226526 26.809018829017027 25 28.163539905056478 23.60720796231566 22.657760337394567 25.571491795233296 26 27.603515109756454 23.49542115341803 23.326692194183668 25.57437154264185 27 26.92127113510457 23.77419475011443 23.121277098426557 26.183257016354446 28 27.05358802272976 24.253085313308876 23.163259986997755 25.53006667696361 29 27.472748961290534 23.399682979887146 23.328271854765976 25.799296204056336 30 26.811226597420145 23.826629225449405 23.184024091491633 26.178120085638817 31 27.69429513523684 23.743405275680296 22.88162499025795 25.68067459882491 32 26.48709657785283 23.73778141264322 23.391512160097466 26.38360984940648 33 27.29951137374817 23.342741675735738 23.23515625498631 26.122590695529784 34 27.5350570405265 23.79215757208379 23.053378430549458 25.61940695684025 35 26.386520787695478 23.705558046938894 24.15189173538456 25.75602942998107 36 26.484082654264036 23.43531044653239 23.79943454388978 26.281172355313792 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 615.0 1 375.0 2 135.0 3 135.0 4 187.0 5 239.0 6 239.0 7 455.5 8 672.0 9 672.0 10 1338.5 11 2005.0 12 2005.0 13 3910.0 14 5815.0 15 10107.0 16 14399.0 17 14399.0 18 22599.0 19 30799.0 20 30799.0 21 46773.0 22 62747.0 23 62747.0 24 90133.0 25 117519.0 26 166066.5 27 214614.0 28 214614.0 29 305849.5 30 397085.0 31 397085.0 32 556180.0 33 715275.0 34 715275.0 35 977151.5 36 1239028.0 37 1239028.0 38 1667270.5 39 2095513.0 40 2671750.5 41 3247988.0 42 3247988.0 43 3989001.5 44 4730015.0 45 4730015.0 46 5584817.5 47 6439620.0 48 6439620.0 49 7133915.0 50 7828210.0 51 8268874.0 52 8709538.0 53 8709538.0 54 8817278.5 55 8925019.0 56 8925019.0 57 8796677.5 58 8668336.0 59 8668336.0 60 7676076.0 61 6683816.0 62 6683816.0 63 5763655.0 64 4843494.0 65 4009894.0 66 3176294.0 67 3176294.0 68 2512163.5 69 1848033.0 70 1848033.0 71 1475964.5 72 1103896.0 73 1103896.0 74 886920.5 75 669945.0 76 513563.0 77 357181.0 78 357181.0 79 293838.0 80 230495.0 81 230495.0 82 183364.0 83 136233.0 84 136233.0 85 102311.5 86 68390.0 87 68390.0 88 51275.5 89 34161.0 90 21770.5 91 9380.0 92 9380.0 93 6508.0 94 3636.0 95 3636.0 96 1976.0 97 316.0 98 316.0 99 197.5 100 79.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29477678411266356 2 0.009257609794501583 3 0.0035201503473290758 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.002598796441866184 21 0.003752898942281585 22 6.8860531051038255E-6 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 5.50884248408306E-6 31 4.407073987266448E-5 32 0.14674454609100454 33 0.09389546571995372 34 0.1514614924680007 35 0.1262874595263621 36 1.7215132762759563E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 7.2610535E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.61136232029831 #Duplication Level Percentage of deduplicated Percentage of total 1 79.04350401474343 36.052819006824166 2 10.052600135087905 9.170255740451482 3 3.6621594458923123 5.011082440838914 4 1.9250755255281027 3.5122126915520386 5 1.178681282165643 2.6880629510505454 6 0.7641801684829933 2.091317912559865 7 0.5397837869841225 1.723419171792888 8 0.4063113329602175 1.4825930737993476 9 0.3207131214465812 1.3165346144856462 >10 1.8363028479544221 15.449905892326242 >50 0.14551428703832547 4.592132894100733 >100 0.11356776424673927 9.573832426445154 >500 0.007378530745992716 2.292735993030905 >1k 0.003977527022732862 3.3186715193130767 >5k 1.658245862168544E-4 0.49394859048480494 >10k+ 8.440511467531939E-5 1.2304750809442835 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT 522279 0.7192881859361041 Illumina Paired End PCR Primer 2 (96% over 25bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.0658159315311477E-5 1.377210621020765E-6 0.0 1.377210621020765E-6 0.0 2 3.580747614653989E-5 3.16758442834776E-5 0.0 6.8860531051038255E-6 5.50884248408306E-6 3 9.08959009873705E-5 3.3053054904498364E-5 0.0 6.8860531051038255E-6 1.6526527452249182E-5 4 2.4238906929965466E-4 3.3053054904498364E-5 0.0 1.101768496816612E-5 2.8921423041436066E-5 5 2.850825985512984E-4 3.443026552551913E-5 0.0 1.101768496816612E-5 3.16758442834776E-5 6 3.305305490449836E-4 3.443026552551913E-5 0.0 1.5149316831228417E-5 3.443026552551913E-5 7 3.5394312960233663E-4 4.131631863062295E-5 0.0 1.7903738073269946E-5 3.443026552551913E-5 8 4.296897137584787E-4 4.957958235674754E-5 0.0 2.203536993633224E-5 5.784284608287213E-5 9 5.192084041248284E-4 4.957958235674754E-5 0.0 2.7544212420415302E-5 6.610610980899673E-5 10 7.450709459722339E-4 6.472889918797596E-5 0.0 2.8921423041436066E-5 7.023774167205903E-5 11 9.37880432915141E-4 7.023774167205903E-5 0.0 3.8561897388581426E-5 7.712379477716285E-5 12 0.001284937509412374 7.161495229307978E-5 0.0 3.993910800960219E-5 8.400984788226668E-5 13 0.0014887646813234472 7.574658415614208E-5 0.0 3.993910800960219E-5 1.0329079657655738E-4 14 0.0016622932195720635 7.987821601920438E-5 0.0 4.6825161114706015E-5 1.0604521781859891E-4 15 0.02255182391921503 8.26326372612459E-5 0.0 4.6825161114706015E-5 1.1981732402880656E-4 16 0.023006303424151883 8.26326372612459E-5 0.0 5.233400359878908E-5 1.2945779837595191E-4 17 0.02385191074545863 8.67642691243082E-5 0.0 5.92200567038929E-5 1.335894302390142E-4 18 0.02572216276880483 8.67642691243082E-5 0.0 6.0597267324913666E-5 1.4460711520718032E-4 19 0.02971469635914403 9.365032222941203E-5 0.0 6.0597267324913666E-5 1.542475895543257E-4 20 0.7759659118335928 9.502753285043279E-5 0.0 6.197447794593443E-5 1.735285382486164E-4 21 0.7897201143057271 1.1293127092370274E-4 0.0 6.197447794593443E-5 1.7628295949065792E-4 22 0.7914526452669712 1.1568569216574426E-4 0.0 6.610610980899673E-5 1.8316901259576176E-4 23 0.793292598656655 1.2394895589186886E-4 0.0 6.748332043001748E-5 1.9831832942699017E-4 24 0.7946546599608445 1.267033771339104E-4 0.0 7.574658415614208E-5 2.0382717191107323E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGGTT 63825 0.0 25.96658 30 AATGCCG 66695 0.0 25.694735 13 GATCGGA 65900 0.0 25.411776 21 CGAGATC 66060 0.0 25.34857 18 CGGTTCA 68410 0.0 25.334372 1 TGCCGAG 69290 0.0 24.751247 15 GAGCGGT 67020 0.0 24.72642 29 GAGATCG 67800 0.0 24.693607 19 AGATCGG 69040 0.0 24.247923 20 TCGGAAG 69715 0.0 24.039654 23 GCCGAGA 71350 0.0 24.015615 16 AAGAGCG 69350 0.0 23.999426 27 CCGAGAT 70195 0.0 23.904486 17 ATGCCGA 74025 0.0 23.590548 14 CGGAAGA 71345 0.0 23.412663 24 ATCGGAA 71515 0.0 23.394749 22 AGAGCGG 71675 0.0 23.27139 28 GAATGCC 74975 0.0 22.995083 12 AGGAATG 80840 0.0 21.606855 10 CAGGAAT 82140 0.0 21.319654 9 >>END_MODULE