##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR873434.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65905457 Sequences flagged as poor quality 0 Sequence length 36 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811962141465767 31.0 31.0 34.0 30.0 34.0 2 32.184766991297856 33.0 31.0 34.0 30.0 34.0 3 32.37638194360749 34.0 31.0 34.0 30.0 34.0 4 35.674039541217354 37.0 35.0 37.0 35.0 37.0 5 35.7110692063026 37.0 35.0 37.0 35.0 37.0 6 35.74166143783815 37.0 35.0 37.0 35.0 37.0 7 35.72440908800617 37.0 35.0 37.0 35.0 37.0 8 35.792682114320215 37.0 35.0 37.0 35.0 37.0 9 37.505534040982376 39.0 37.0 39.0 35.0 39.0 10 37.45968542483516 39.0 37.0 39.0 35.0 39.0 11 37.55740789415966 39.0 37.0 39.0 35.0 39.0 12 37.41732365197012 39.0 37.0 39.0 34.0 39.0 13 37.28952393122773 39.0 37.0 39.0 34.0 39.0 14 38.59312765254021 40.0 38.0 41.0 34.0 41.0 15 38.56088176431278 40.0 38.0 41.0 34.0 41.0 16 38.52487243962211 40.0 38.0 41.0 34.0 41.0 17 38.36457985565596 40.0 38.0 41.0 34.0 41.0 18 38.37200201798161 40.0 38.0 41.0 34.0 41.0 19 38.370925976584914 40.0 38.0 41.0 34.0 41.0 20 37.83927523634348 40.0 37.0 41.0 32.0 41.0 21 37.95654783184342 40.0 38.0 41.0 33.0 41.0 22 37.73354878640778 39.0 37.0 41.0 32.0 41.0 23 37.806634145636835 39.0 37.0 41.0 33.0 41.0 24 37.777397993006865 39.0 37.0 41.0 33.0 41.0 25 37.859131194553434 39.0 37.0 41.0 33.0 41.0 26 37.63568212568498 39.0 37.0 41.0 33.0 41.0 27 37.45645062441491 39.0 37.0 40.0 32.0 41.0 28 37.34214679673642 39.0 37.0 40.0 32.0 41.0 29 37.11590988588396 39.0 37.0 40.0 31.0 41.0 30 36.64569795790962 39.0 36.0 40.0 30.0 41.0 31 37.242338081351896 39.0 37.0 40.0 32.0 41.0 32 37.046510837486494 39.0 37.0 40.0 31.0 41.0 33 37.33636557591885 39.0 37.0 41.0 31.0 41.0 34 37.36457933976545 40.0 37.0 41.0 32.0 41.0 35 36.88533193844631 39.0 37.0 40.0 31.0 41.0 36 36.32555443777592 38.0 36.0 40.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.16290718210148114 1101 2 -0.175501601354064 1101 3 -0.1259048205099802 1101 4 -0.10823107833366663 1101 5 -0.13136878298838184 1101 6 -0.11644646052950236 1101 7 -0.10625311897795342 1101 8 -0.10448521747996153 1101 9 -0.11961743818719839 1101 10 -0.15616737853542872 1101 11 -0.16997583296998897 1101 12 -0.1777083230611538 1101 13 -0.16611543560670583 1101 14 -0.21144497185999 1101 15 -0.28824941150872974 1101 16 -0.2790852623132949 1101 17 -0.2830277154503449 1101 18 -0.3617170312261493 1101 19 -0.3229425770823795 1101 20 -0.4215297260073285 1101 21 -0.4168541129736454 1101 22 -0.4323290175498613 1101 23 -0.4857773837085517 1101 24 -0.554962701927856 1101 25 -0.5733889520640716 1101 26 -0.6274115614098292 1101 27 -0.5846444042963768 1101 28 -0.6865918373859117 1101 29 -0.7317395767782671 1101 30 -0.8640377986247216 1101 31 -0.6478015969590842 1101 32 -0.6717296353647413 1101 33 -0.6416637257383542 1101 34 -0.6320089338888266 1101 35 -0.6937568880188678 1101 36 -0.8767942543875904 1102 1 -0.18901625553003143 1102 2 -0.17841168866160473 1102 3 -0.13515633510886715 1102 4 -0.13931337048457948 1102 5 -0.13299180636200703 1102 6 -0.15829174803473478 1102 7 -0.14918671746879397 1102 8 -0.11441400825096792 1102 9 -0.1316539512656547 1102 10 -0.19601708880962576 1102 11 -0.14296271912397174 1102 12 -0.16138274908882266 1102 13 -0.19085733676038785 1102 14 -0.2789642624971407 1102 15 -0.2645364940913666 1102 16 -0.26867497414562536 1102 17 -0.31764693704648295 1102 18 -0.31964789689145334 1102 19 -0.2783127233858451 1102 20 -0.45692002958850964 1102 21 -0.4413212958949728 1102 22 -0.46036108640784335 1102 23 -0.45891774020314813 1102 24 -0.451822630676908 1102 25 -0.4508008802718422 1102 26 -0.48097694536786406 1102 27 -0.4420521083470135 1102 28 -0.5184026718671362 1102 29 -0.49915786543108 1102 30 -0.6516083320296175 1102 31 -0.4599789354636741 1102 32 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1107 28 -0.4201487703795692 1107 29 -0.41319276639902114 1107 30 -0.49418719917549225 1107 31 -0.4842869754803445 1107 32 -0.358979034446115 1107 33 -0.47676951858233707 1107 34 -0.5114426694459908 1107 35 -0.5433408147624306 1107 36 -0.44607795480108337 1108 1 -0.45497888740386827 1108 2 -0.518977879804698 1108 3 -0.47210786861792187 1108 4 -0.35004421317973566 1108 5 -0.424248184050235 1108 6 -0.37273064883273577 1108 7 -0.41767006536201734 1108 8 -0.38090447600090016 1108 9 -0.4143631924802236 1108 10 -0.6493540235821484 1108 11 -0.5691347103348647 1108 12 -0.5983873246883746 1108 13 -0.7480449962674598 1108 14 -0.8480287182498216 1108 15 -0.6483561274209322 1108 16 -0.6828653119469621 1108 17 -0.6154210083715483 1108 18 -0.5472851760706661 1108 19 -0.6426619491822336 1108 20 -0.963768465273823 1108 21 -0.8622091872326436 1108 22 -1.0257215319955435 1108 23 -0.672525208256431 1108 24 -0.7472787069479878 1108 25 -0.798097604608067 1108 26 -0.8297527076457527 1108 27 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32 0.35712306081543943 2207 33 0.3507800650256456 2207 34 0.40865416972252433 2207 35 0.4253221312283273 2207 36 0.5758521306627813 2208 1 -0.034964179293496045 2208 2 0.001092088180918438 2208 3 0.0012758249212296846 2208 4 0.008589552555136493 2208 5 0.03702157558542751 2208 6 -0.040780552089685784 2208 7 -0.0020225610622617296 2208 8 0.043969080988333076 2208 9 0.00656990714439587 2208 10 0.12879222770891374 2208 11 0.035263613724353604 2208 12 0.08976157786725025 2208 13 0.09503445035747404 2208 14 0.15918353831349918 2208 15 0.16614688157722668 2208 16 -0.061867289999447905 2208 17 -0.05657112959966781 2208 18 0.014417899607337858 2208 19 -0.027720782933933208 2208 20 0.26215697232430557 2208 21 -0.02892052335134565 2208 22 0.05293210416018468 2208 23 0.06041995011842971 2208 24 0.1963800790367074 2208 25 0.19897615033555383 2208 26 0.27467374048467263 2208 27 0.3247846664322793 2208 28 0.2996106936597229 2208 29 0.27306062337473236 2208 30 0.20126797080274628 2208 31 0.13440380541175045 2208 32 0.05234162345944071 2208 33 0.13771980773751125 2208 34 0.2222202823737902 2208 35 0.31244103116035404 2208 36 0.26631266101821893 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 138048.0 3 106.0 4 448.0 5 830.0 6 1087.0 7 1402.0 8 1981.0 9 2471.0 10 3058.0 11 3738.0 12 4482.0 13 5785.0 14 7289.0 15 9158.0 16 12019.0 17 15509.0 18 20719.0 19 27914.0 20 38097.0 21 47456.0 22 60912.0 23 80088.0 24 109093.0 25 157233.0 26 234975.0 27 342354.0 28 490688.0 29 702540.0 30 972040.0 31 1295954.0 32 1693768.0 33 2210238.0 34 2942645.0 35 4047059.0 36 5957612.0 37 1.000142E7 38 2.1281117E7 39 1.2984124E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32990436954833 18.412852995865574 6.18624903841614 32.070993596169956 2 37.08878492079254 18.542999416317567 29.963708925533872 14.404506737356016 3 37.01599853863391 19.78254547267611 22.696252603179733 20.505203385510246 4 20.869552577414037 27.666191587139743 34.88019967754718 16.584056157899035 5 22.25954521489776 27.23059032880995 34.456476039609285 16.053388416683006 6 18.880635331911893 26.655918644187537 17.722216841619048 36.74122918228152 7 17.754063976826686 31.280021319023703 31.06648816652618 19.899426537623434 8 34.22431317030394 17.560887257029414 22.007516008879204 26.207283563787442 9 19.876706112515084 17.37496335091038 22.186930590588272 40.56139994598627 10 20.75263964864093 43.85812543565247 18.193215775743727 17.19601913996287 11 40.36425238656641 20.61125681899148 16.483452955951737 22.541037838490368 12 37.39856473493538 17.714760706385814 21.466832405092042 23.419842153586764 13 20.06957633265482 36.08448993836732 23.126617572805845 20.719316156172017 14 20.39418829915708 36.709917662812046 22.213309286361522 20.68258475166935 15 20.97122094153751 20.336077784879027 37.35358818618009 21.33911308740337 16 38.14813088998078 20.29888207891495 20.737618130771782 20.815368900332487 17 21.542956602212772 20.86451505829024 20.428179414642404 37.164348924854586 18 21.18620162212061 20.64861487873455 20.762154793949765 37.40302870519508 19 37.6952177234864 20.91230599689528 20.253252792299296 21.139223487319022 20 21.9443770455808 37.07479726093042 20.35714367004794 20.623682023440846 21 37.74143783644532 21.041752324351968 20.32166710621827 20.89514273298444 22 21.613659396975276 37.87367713723614 20.204050781409496 20.308612684379078 23 21.16121431340655 21.787805826154884 36.58334544285157 20.467634417587 24 21.63266692771738 21.066782072385905 20.541537250853143 36.759013749043575 25 37.69865827682781 21.64020560598883 19.92051627769723 20.740619839486133 26 37.822670382415794 21.20983819171545 20.453743597915455 20.5137478279533 27 21.505884559415964 36.967763059601445 20.307418653334096 21.218933727648498 28 21.777781413994525 37.559898470014744 20.232485093185247 20.429835022805477 29 37.693232906942704 21.41363237884718 20.364642196800155 20.528492517409962 30 21.63399523931302 36.8552528159046 20.79769484250513 20.71305710227725 31 37.22638404766458 21.620462005188486 20.599241558849414 20.55391238829752 32 21.482250700161867 21.24690903337027 20.44893724038111 36.82190302608676 33 37.27429919865713 21.443529447290565 20.255952029593647 21.02621932445866 34 38.09142685464702 21.38183616940082 20.276899522866955 20.249837453085203 35 21.153842243814175 21.382843129355884 36.62442674291654 20.838887883913408 36 20.92087807674409 21.501704967294337 36.67723549639789 20.90018145956368 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2606.0 1 2143.0 2 1680.0 3 1680.0 4 2656.0 5 3632.0 6 3632.0 7 6040.5 8 8449.0 9 8449.0 10 14439.5 11 20430.0 12 20430.0 13 31568.0 14 42706.0 15 61340.0 16 79974.0 17 79974.0 18 108110.0 19 136246.0 20 136246.0 21 181695.0 22 227144.0 23 227144.0 24 292863.0 25 358582.0 26 448365.5 27 538149.0 28 538149.0 29 734468.0 30 930787.0 31 930787.0 32 1172601.0 33 1414415.0 34 1414415.0 35 1778439.5 36 2142464.0 37 2142464.0 38 2645957.5 39 3149451.0 40 3631050.5 41 4112650.0 42 4112650.0 43 4586714.0 44 5060778.0 45 5060778.0 46 5403185.5 47 5745593.0 48 5745593.0 49 5859794.5 50 5973996.0 51 5863165.0 52 5752334.0 53 5752334.0 54 5557518.0 55 5362702.0 56 5362702.0 57 1.0115933E7 58 1.4869164E7 59 1.4869164E7 60 9284696.5 61 3700229.0 62 3700229.0 63 3021779.5 64 2343330.0 65 1898783.0 66 1454236.0 67 1454236.0 68 1175322.0 69 896408.0 70 896408.0 71 743962.0 72 591516.0 73 591516.0 74 508330.0 75 425144.0 76 322020.0 77 218896.0 78 218896.0 79 188746.0 80 158596.0 81 158596.0 82 127783.0 83 96970.0 84 96970.0 85 72630.0 86 48290.0 87 48290.0 88 37062.5 89 25835.0 90 16583.5 91 7332.0 92 7332.0 93 5845.0 94 4358.0 95 4358.0 96 2344.5 97 331.0 98 331.0 99 192.5 100 54.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18323672347799666 2 0.010028001171435623 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 6.069300149151534E-6 20 0.006444079433361641 21 0.0017813395937759753 22 0.0 23 0.0 24 0.0 25 0.01739157957739372 26 0.05831687048312251 27 0.037150186212956536 28 0.03796043778286827 29 0.01602295239376005 30 0.01952190392974591 31 0.008023614797178327 32 0.0032243157042367524 33 0.007324127954988614 34 0.002134876327464052 35 5.310637630507592E-5 36 0.0014672533110573834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 6.5905457E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.52950082643807 #Duplication Level Percentage of deduplicated Percentage of total 1 75.7182924733455 26.145148425348623 2 13.529834962615128 9.343568950463792 3 4.345182715716982 4.501109705201217 4 1.9633825282145474 2.7117847452239308 5 1.1011649359450646 1.9011337782879862 6 0.6946246518821697 1.439102549473778 7 0.4753520210966715 1.1489567603714568 8 0.35372785401396833 0.9771236982007585 9 0.25052402141000707 0.7785422463887484 >10 1.3419012164454889 8.376354321258535 >50 0.09816573091447904 2.3507242064165155 >100 0.07197266487099307 5.138144827342509 >500 0.04026334904566096 10.346104053990794 >1k 0.01508916879443709 7.268221267089355 >5k 3.6826327973327265E-4 0.8582061645294745 >10k+ 1.534424092433419E-4 16.71577430041272 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT 10090867 15.311125147042073 Illumina Paired End PCR Primer 2 (96% over 25bp) CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCA 263804 0.40027641413669285 Illumina Paired End PCR Primer 2 (100% over 22bp) CGGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGT 72460 0.10994537220188004 Illumina Paired End PCR Primer 2 (95% over 24bp) CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAA 67485 0.10239668014137282 Illumina Paired End PCR Primer 2 (96% over 33bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.2485101044060737E-5 0.0 0.0 1.5173250372878834E-6 4.551975111863651E-6 2 6.524497660337899E-5 7.586625186439417E-6 0.0 6.069300149151533E-6 6.069300149151533E-6 3 1.7600970432539448E-4 7.586625186439417E-6 0.0 9.103950223727301E-6 2.4277200596606134E-5 4 3.64158008949092E-4 7.586625186439417E-6 1.5173250372878834E-6 1.2138600298303067E-5 2.8829175708469785E-5 5 4.0967776006772854E-4 7.586625186439417E-6 1.5173250372878834E-6 1.2138600298303067E-5 2.8829175708469785E-5 6 4.870613369694106E-4 7.586625186439417E-6 1.5173250372878834E-6 1.2138600298303067E-5 2.8829175708469785E-5 7 5.280291129761835E-4 1.0621275261015184E-5 1.5173250372878834E-6 1.8207900447454603E-5 2.8829175708469785E-5 8 6.312072155117595E-4 1.9725225484742486E-5 1.5173250372878834E-6 2.7311850671181903E-5 4.855440119321227E-5 9 7.996302946507146E-4 1.9725225484742486E-5 1.5173250372878834E-6 2.7311850671181903E-5 5.765835141693957E-5 10 0.0012927609317692768 2.275987555931825E-5 1.5173250372878834E-6 2.8829175708469785E-5 6.0693001491515336E-5 11 0.00426520067981624 2.579452563389402E-5 1.5173250372878834E-6 3.3381150820333433E-5 6.221032652880323E-5 12 0.00930575445368659 2.579452563389402E-5 1.5173250372878834E-6 4.0967776006772854E-5 6.827962667795476E-5 13 0.011053712896642231 2.579452563389402E-5 1.5173250372878834E-6 4.400242608134862E-5 8.497020208812147E-5 14 0.012212949225130174 2.7311850671181903E-5 1.5173250372878834E-6 5.007172623050016E-5 8.497020208812147E-5 15 0.4423518374206858 2.7311850671181903E-5 3.0346500745757667E-6 5.614102637965169E-5 9.1039502237273E-5 16 0.448008425159695 2.7311850671181903E-5 3.0346500745757667E-6 5.917567645422745E-5 9.710880238642453E-5 17 0.46262026526877736 3.0346500745757668E-5 3.0346500745757667E-6 6.524497660337899E-5 1.0317810253557608E-4 18 0.4883510632511053 3.186382578304555E-5 3.0346500745757667E-6 6.827962667795476E-5 1.0469542757286396E-4 19 0.5532030526698267 3.489847585762132E-5 3.0346500745757667E-6 6.979695171524265E-5 1.1076472772201549E-4 20 16.33340771766441 3.6415800894909206E-5 3.0346500745757667E-6 7.283160178981841E-5 1.2593797809489434E-4 21 16.5924575866305 4.855440119321227E-5 3.0346500745757667E-6 7.283160178981841E-5 1.3048995320675798E-4 22 16.605392782573375 5.310637630507592E-5 3.0346500745757667E-6 7.434892682710629E-5 1.3200727824404587E-4 23 16.61582439220473 5.310637630507592E-5 3.0346500745757667E-6 7.434892682710629E-5 1.3200727824404587E-4 24 16.619980345481864 5.310637630507592E-5 3.0346500745757667E-6 7.434892682710629E-5 1.3504192831862162E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAGCAG 1122110 0.0 28.538195 5 CGGTTCA 1121185 0.0 28.531227 1 AGCGGTT 1100630 0.0 28.409805 30 TCGGAAG 1120410 0.0 28.189278 23 GGTTCAG 1141070 0.0 28.129799 2 AATGCCG 1131995 0.0 28.121674 13 GTTCAGC 1141040 0.0 28.104433 3 AGATCGG 1126825 0.0 28.09935 20 CGAGATC 1126980 0.0 28.087248 18 GAGATCG 1129115 0.0 28.061436 19 ATGCCGA 1132790 0.0 28.060398 14 GATCGGA 1129305 0.0 28.052502 21 AGGAATG 1140610 0.0 28.041666 10 GGAATGC 1139515 0.0 28.011103 11 GCCGAGA 1133695 0.0 28.005812 16 ATCGGAA 1128305 0.0 28.003067 22 TTCAGCA 1144335 0.0 27.999395 4 TGCCGAG 1135350 0.0 27.993654 15 CGGAAGA 1122905 0.0 27.988611 24 GAATGCC 1139030 0.0 27.984453 12 >>END_MODULE