FastQCFastQC Report
Fri 27 May 2016
ERR765574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12608924
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA640960.5083383800235453No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT627240.497457197775163No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG260560.2066472920290423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215000.17051415330919592No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG192010.15228103524138936No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC188260.1493069511720429No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT186070.1475700860755446No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC185450.14707837084274597No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA181540.1439773925197741No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG174150.1381164641804487No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC171160.13574512781582315No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC132860.1053698158542315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131850.10456879587822086No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC126780.10054783421646447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG67850.024.9928821
GTATAAG71500.023.1914351
GTGATCG50500.023.1579328
ACGTTAA18850.022.424518
TAGGCAT101200.020.6070085
TGATCGC58250.019.9962049
TAGTACT79700.019.9205744
CTAGGCA110300.018.9934064
CGTTAAC22050.018.9571579
CTGTGCG82000.018.729489
ATAAGGT71900.018.6848343
GTACATA33500.018.649441
CTAGTAC90950.018.3348673
GACGTTA24150.018.0869167
GCATCGT83000.017.7681968
TCTAACG39450.017.7416112
CATGGGG312750.017.572444
TAAGGTG72550.017.4811444
TATTCGC14800.017.4539139
CTATTGA61050.017.3865979
Produced by FastQC (version 0.11.5)