##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765574_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12608924
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.894788960580616	33.0	33.0	33.0	27.0	33.0
2	31.783091007606995	33.0	33.0	33.0	27.0	33.0
3	31.80156308341616	33.0	33.0	33.0	27.0	33.0
4	35.54098454396267	37.0	37.0	37.0	33.0	37.0
5	35.60887780749571	37.0	37.0	37.0	33.0	37.0
6	35.629868972166065	37.0	37.0	37.0	33.0	37.0
7	35.44687627588207	37.0	37.0	37.0	33.0	37.0
8	35.41676085921368	37.0	37.0	37.0	33.0	37.0
9	35.24959544525766	37.0	37.0	37.0	33.0	37.0
10-11	35.24619317239124	37.0	37.0	37.0	33.0	37.0
12-13	35.20489924437644	37.0	37.0	37.0	33.0	37.0
14-15	36.837432638978555	40.0	37.0	40.0	33.0	40.0
16-17	36.89140132020781	40.0	37.0	40.0	33.0	40.0
18-19	36.85177331547085	40.0	37.0	40.0	33.0	40.0
20-21	36.56731117579898	40.0	37.0	40.0	33.0	40.0
22-23	36.63566744473994	40.0	37.0	40.0	33.0	40.0
24-25	36.54259054936012	40.0	37.0	40.0	33.0	40.0
26-27	36.51212756140016	40.0	37.0	40.0	33.0	40.0
28-29	36.46359847993374	40.0	37.0	40.0	33.0	40.0
30-31	36.574069325820346	37.0	37.0	40.0	33.0	40.0
32-33	36.333720228625374	37.0	37.0	40.0	33.0	40.0
34-35	36.17201277444451	37.0	37.0	40.0	27.0	40.0
36-37	36.29315705289365	37.0	37.0	40.0	33.0	40.0
38-39	36.22449948940925	37.0	37.0	40.0	30.0	40.0
40-41	36.26745172704665	37.0	37.0	40.0	33.0	40.0
42-43	36.16802325876498	37.0	37.0	40.0	30.0	40.0
44-45	36.075472538338715	37.0	37.0	40.0	27.0	40.0
46-47	35.88287244018601	37.0	37.0	40.0	27.0	40.0
48-49	35.64334145403684	37.0	37.0	40.0	27.0	40.0
50-51	35.48265462619967	37.0	35.0	40.0	27.0	40.0
52-53	35.35440934531765	37.0	33.0	40.0	27.0	40.0
54-55	35.24724504644489	37.0	33.0	40.0	27.0	40.0
56-57	35.09808557018823	37.0	33.0	40.0	27.0	40.0
58-59	34.91671525659129	37.0	33.0	40.0	27.0	40.0
60-61	34.688347475169174	37.0	33.0	40.0	27.0	40.0
62-63	34.45086468123688	37.0	33.0	37.0	27.0	40.0
64-65	34.26005561616519	37.0	33.0	37.0	27.0	40.0
66-67	33.99245903139713	37.0	33.0	37.0	27.0	40.0
68-69	33.77818622746874	37.0	33.0	37.0	27.0	40.0
70-71	33.480169045352326	37.0	33.0	37.0	27.0	40.0
72-73	33.23907579266874	37.0	33.0	37.0	27.0	37.0
74-75	33.03311059690739	37.0	33.0	37.0	27.0	37.0
76-77	32.74206692815342	37.0	33.0	37.0	22.0	37.0
78-79	32.64329842895397	37.0	33.0	37.0	22.0	37.0
80-81	32.488933790068046	37.0	33.0	37.0	22.0	37.0
82-83	32.322372472068196	33.0	33.0	37.0	22.0	37.0
84-85	32.16109859969019	33.0	33.0	37.0	22.0	37.0
86-87	31.988325807975365	33.0	33.0	37.0	22.0	37.0
88-89	31.85246691153028	33.0	33.0	37.0	22.0	37.0
90-91	31.719713315743675	33.0	33.0	37.0	22.0	37.0
92-93	31.57605974149737	33.0	33.0	37.0	22.0	37.0
94-95	31.445919017356278	33.0	33.0	37.0	22.0	37.0
96-97	31.307985122283235	33.0	33.0	37.0	15.0	37.0
98-99	31.192046006463357	33.0	33.0	37.0	15.0	37.0
100	30.95971218479864	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	17.0
7	319.0
8	1562.0
9	3174.0
10	4604.0
11	7491.0
12	13458.0
13	22830.0
14	35377.0
15	43052.0
16	49200.0
17	57147.0
18	65397.0
19	73269.0
20	81893.0
21	94430.0
22	106823.0
23	107995.0
24	106454.0
25	111311.0
26	125529.0
27	147160.0
28	180193.0
29	226067.0
30	282491.0
31	359543.0
32	473813.0
33	643882.0
34	878727.0
35	1254913.0
36	2069800.0
37	3438425.0
38	1540818.0
39	1758.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.75946807670997	19.713350950942377	13.100655563844796	24.426525408502854
2	14.888200391826265	21.790099236691475	38.56143242075615	24.760267950726114
3	17.69876218970568	27.715897001403988	30.061961503266225	24.52337930562411
4	12.598975537895745	18.48457623847865	37.71807851766531	31.198369705960292
5	12.802042386954081	38.71539007169835	34.38413075997907	14.098436781368488
6	29.444311112048755	38.11801349923091	18.401477996215547	14.036197392504787
7	25.387398210297217	33.25569503530571	23.345868741379032	18.01103801301804
8	26.22761866879338	33.86474416605164	20.834572967630972	19.07306419752401
9	26.707892071604356	15.066981369257649	20.465399766669602	37.7597267924684
10-11	24.102171867554844	26.516261724284295	27.727317717239096	21.654248690921758
12-13	25.67699714604199	24.57523532166609	27.346612149208433	22.401155383083488
14-15	23.67831207296909	24.672423998763087	25.6135490859691	26.035714842298724
16-17	22.414563777157504	27.468124943590816	26.78887110407826	23.328440175173423
18-19	22.820588034332555	27.015623628777053	28.605162621068636	21.55862571582175
20-21	23.83886620354972	26.237257367147997	27.897628024355893	22.026248404946383
22-23	23.63241546833293	26.20399186879146	26.92820737437286	23.235385288502755
24-25	23.139513165695384	26.67451896508156	27.1125775821349	23.07339028708816
26-27	23.381626040459192	26.26284638473453	27.09522960309681	23.260297971709473
28-29	22.967633072265066	26.21411977342825	27.852978270332873	22.96526888397381
30-31	23.517229328286966	26.318497372257937	27.490031796303725	22.674241503151364
32-33	22.83078442940275	26.662351761753776	27.257438926747007	23.249424882096466
34-35	22.70116740325703	26.833465726658844	27.78939539182361	22.67597147826051
36-37	23.495301850478423	26.271501489749088	27.211774065929006	23.021422593843482
38-39	23.60007667954101	26.018576697909506	27.23167893362118	23.149667688928304
40-41	22.946813428225628	26.071664163919145	27.6573291412643	23.32419326659093
42-43	23.524851561837625	26.083192817123173	27.063634333397722	23.328321287641472
44-45	23.441559152556653	26.262034193462082	27.092837666349133	23.203568987632128
46-47	23.019241506019274	26.247975158265053	27.490046036523186	23.24273729919249
48-49	23.54873726143893	26.315922067164855	27.14329598999082	22.992044681405396
50-51	22.70636680262082	26.699899022263185	26.68428341751319	23.90945075760281
52-53	22.425647045775186	26.920117406896516	26.08150635107778	24.572729196250513
54-55	22.575121821152752	26.806714615817224	25.98875587368632	24.629407689343704
56-57	23.383750876751538	26.02854596716503	26.031005075758788	24.55669808032464
58-59	22.66853214529712	26.05772340874904	27.127435922098087	24.14630852385575
60-61	23.076262228630018	26.420567697973414	26.470110878200565	24.033059195196003
62-63	22.568554572779167	26.252965856943415	26.514885021270718	24.6635945490067
64-65	22.02225533246949	26.464064108151724	26.786850676574993	24.72682988280379
66-67	23.26629506466279	26.01681811959792	26.337646036814927	24.379240778924363
68-69	22.92287944963248	25.739966895127047	26.536252929797122	24.800900725443352
70-71	22.50510934173781	26.144022907113506	26.42860463444784	24.92226311670084
72-73	23.285322197526373	25.855199472138636	26.458983549550368	24.400494780784623
74-75	23.333380664454374	25.881110464252455	26.468558425469705	24.316950445823462
76-77	22.74605959133929	26.113659198832462	26.69129743942801	24.448983770400243
78-79	22.943350164507237	26.24828584169997	26.434674093056408	24.373689900736387
80-81	22.499419315471307	26.33035460151238	26.64839044085379	24.52183564216252
82-83	22.712027826776232	25.886500925207066	26.558172977131157	24.84329827088554
84-85	22.888672825890094	26.16803527573987	25.91543672196397	25.027855176406067
86-87	22.631605043853472	25.820847922399327	26.40378911776991	25.143757915977293
88-89	22.940402178236706	25.125037108119614	26.616259917249092	25.31830079639459
90-91	22.96469519009559	25.56320865925752	26.320329632886114	25.151766517760777
92-93	22.830514155101564	25.400014351713697	26.513783496108395	25.255687997076343
94-95	22.123304350288876	25.540329647405773	26.965167470863875	25.37119853144148
96-97	22.8606951596317	25.35595050164613	26.284235041812178	25.499119296909996
98-99	22.83082491739227	25.527014876273558	26.031802750765266	25.610357455568906
100	22.680205752207122	25.477458263610693	26.238484358722292	25.60385162545989
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2508.0
1	2133.0
2	2187.0
3	2653.0
4	2222.5
5	1849.0
6	2334.0
7	4191.5
8	6824.5
9	6761.5
10	4271.0
11	2748.0
12	2498.5
13	2705.0
14	2998.0
15	3519.5
16	4260.0
17	5182.0
18	6411.0
19	7816.0
20	9578.5
21	12167.5
22	15639.5
23	19840.0
24	24887.5
25	31207.0
26	40256.0
27	51186.5
28	63391.5
29	80555.5
30	100949.5
31	121999.5
32	145484.5
33	167392.0
34	188865.5
35	207135.0
36	225135.5
37	253776.0
38	276487.5
39	296165.0
40	307271.5
41	313303.5
42	328813.5
43	356330.5
44	395969.5
45	447811.5
46	503151.5
47	552027.5
48	608641.0
49	646105.5
50	705940.5
51	707601.5
52	606718.5
53	541429.5
54	504163.5
55	453930.5
56	406698.0
57	347605.0
58	290947.5
59	258447.0
60	216608.0
61	170647.5
62	132873.5
63	103461.5
64	76736.5
65	52281.0
66	37813.0
67	28864.0
68	21759.0
69	16372.5
70	12905.5
71	10250.0
72	8200.0
73	6897.0
74	5503.0
75	4203.5
76	3261.0
77	2449.5
78	1903.0
79	1429.0
80	1088.5
81	864.0
82	732.5
83	616.5
84	452.5
85	299.0
86	199.5
87	126.5
88	80.0
89	56.5
90	47.0
91	34.5
92	24.0
93	18.5
94	14.5
95	8.0
96	5.0
97	5.5
98	3.5
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01759864679967934
2	0.00622574931849855
3	0.00996912980044927
4	0.009017422898258408
5	0.01981136534727309
6	0.003981307207498435
7	0.02404646106202242
8	0.008240195594802538
9	0.0015385928252085585
10-11	4.1240632428270646E-4
12-13	9.437760113392706E-4
14-15	0.0010508430378357422
16-17	0.0012848043179576624
18-19	0.0033745940573517612
20-21	0.006388332580956155
22-23	0.013450790884297502
24-25	0.022591142590755564
26-27	0.027218817402658625
28-29	0.03311146930539037
30-31	0.03546297844288696
32-33	0.03610141515644
34-35	0.045170388845233736
36-37	0.041966308941191174
38-39	0.04618157742881153
40-41	0.04315990801435555
42-43	0.04984961444767214
44-45	0.04331852583138736
46-47	0.045828652785915754
48-49	0.048358606967573124
50-51	0.04874325517387527
52-53	0.03341284315775081
54-55	0.02985980405623826
56-57	0.02165133202484209
58-59	0.021306338272797903
60-61	0.02180201895102231
62-63	0.02176632994219015
64-65	0.02558505388723098
66-67	0.02706416503105261
68-69	0.027988113815262904
70-71	0.028408451030397203
72-73	0.026501071780589684
74-75	0.021421336190145964
76-77	0.02604108011119743
78-79	0.025755568040540175
80-81	0.024680932330149664
82-83	0.02670727494273104
84-85	0.028130869850591534
86-87	0.030763925613319584
88-89	0.030660824032248905
90-91	0.03070047848650686
92-93	0.032972678715487536
94-95	0.03101374867514468
96-97	0.03348422117541513
98-99	0.034281275705999974
100	0.04293784307051102
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2608924E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.95320068083516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.74034220357399	22.322610908228206
2	15.179421025728601	10.004210145594465
3	6.481905877110227	6.407986354880945
4	3.24638867886219	4.279155904901483
5	1.9744347296630202	3.2531971938898008
6	1.1814088177965236	2.335872111537424
7	0.7994065131799701	1.8440102278070378
8	0.5617918287701192	1.4810271099452097
9	0.42195861656887884	1.2514398273721656
>10	2.050848907457492	12.489495988916202
>50	0.18574713929247158	4.249726106165086
>100	0.13634568330660965	9.077850070988886
>500	0.020694249881120463	4.711535351348673
>1k	0.01788399021330713	11.000803942017043
>5k	9.478257303029257E-4	2.0424663597103043
>10k+	4.7391286515146284E-4	3.248612396697286
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	64096	0.5083383800235453	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	62724	0.497457197775163	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	26056	0.2066472920290423	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21500	0.17051415330919592	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	19201	0.15228103524138936	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	18826	0.1493069511720429	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	18607	0.1475700860755446	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	18545	0.14707837084274597	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	18154	0.1439773925197741	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	17415	0.1381164641804487	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	17116	0.13574512781582315	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13286	0.1053698158542315	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13185	0.10456879587822086	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	12678	0.10054783421646447	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5861781703181016E-5
2	0.0	1.5861781703181016E-5	0.0	0.0	1.5861781703181016E-5
3	0.0	1.5861781703181016E-5	0.0	1.5861781703181016E-5	1.5861781703181016E-5
4	0.0	2.3792672554771527E-5	0.0	1.5861781703181016E-5	2.3792672554771527E-5
5	0.0	7.930890851590508E-5	0.0	3.172356340636203E-5	2.3792672554771527E-5
6	0.0	7.930890851590508E-5	0.0	3.172356340636203E-5	3.172356340636203E-5
7	0.0	7.930890851590508E-5	0.0	3.172356340636203E-5	3.172356340636203E-5
8	7.930890851590508E-6	8.723979936749559E-5	0.0	5.551623596113356E-5	3.172356340636203E-5
9	7.930890851590508E-6	9.517069021908611E-5	0.0	1.0310158107067662E-4	3.965445425795254E-5
10-11	7.930890851590508E-6	9.517069021908611E-5	0.0	1.4275603532862914E-4	4.758534510954305E-5
12-13	7.930890851590508E-6	1.1499791734806238E-4	0.0	1.8637593501237695E-4	5.948168138692881E-5
14-15	1.5861781703181016E-5	1.5068692618021965E-4	0.0	2.418921709735105E-4	7.930890851590508E-5
16-17	2.3792672554771527E-5	1.6654870788340067E-4	0.0	2.6965028895407727E-4	1.0706702649647187E-4
18-19	2.3792672554771527E-5	1.9827227128976272E-4	0.0	3.132701886378251E-4	1.387905899028339E-4
20-21	3.965445425795254E-5	2.2206494384453425E-4	0.0	5.353351324823593E-4	1.6654870788340067E-4
22-23	4.7585345109543054E-5	2.2999583469612476E-4	0.0	0.0022523730018517043	2.1413405299294374E-4
24-25	5.155079053533831E-5	2.49823061825101E-4	0.0	0.00624954199105332	2.3792672554771526E-4
26-27	8.723979936749559E-5	2.617193981024868E-4	0.0	0.0106115319594281	2.4189217097351053E-4
28-29	1.1896336277385762E-4	2.617193981024868E-4	0.0	0.0254026433976444	2.4585761639930577E-4
30-31	1.3482514447703863E-4	2.775811798056678E-4	0.0	0.06958167088642933	2.4585761639930577E-4
32-33	1.4275603532862914E-4	2.775811798056678E-4	0.0	0.15564373296246373	2.6965028895407727E-4
34-35	1.4275603532862914E-4	3.013738523604393E-4	0.0	0.272057314327535	2.6965028895407727E-4
36-37	1.4275603532862914E-4	3.727518700247539E-4	0.0	0.44436781441461615	2.8154662523146304E-4
38-39	1.4275603532862914E-4	4.243026605600922E-4	0.0	0.7160444459812748	3.013738523604393E-4
40-41	1.4275603532862914E-4	4.4809533311486374E-4	0.0	1.0587501360147782	3.013738523604393E-4
42-43	1.5068692618021965E-4	4.599916693922495E-4	0.0	1.3537991029210739	3.449937520441871E-4
44-45	1.5465237160601492E-4	4.718880056696353E-4	0.0	1.6881852884512587	3.6482097917316336E-4
46-47	1.5861781703181016E-4	4.7981889652122577E-4	0.0	2.062190239230564	3.6482097917316336E-4
48-49	1.5861781703181016E-4	4.99646123650202E-4	0.0	2.4455457103238945	3.886136517279349E-4
50-51	1.5861781703181016E-4	5.393005779081545E-4	0.0	2.8602757856261167	3.886136517279349E-4
52-53	1.5861781703181016E-4	5.393005779081545E-4	0.0	3.2860337646574758	4.084408788569112E-4
54-55	1.5861781703181016E-4	5.868859230176976E-4	0.0	3.707259239567151	4.282681059858875E-4
56-57	1.5861781703181016E-4	6.106785955724692E-4	0.0	4.175542655344739	4.599916693922495E-4
58-59	1.5861781703181016E-4	6.424021589788312E-4	0.0	4.685713071155002	4.956806782244068E-4
60-61	1.5861781703181016E-4	6.622293861078075E-4	0.0	5.192005281338836	5.075770145017925E-4
62-63	1.5861781703181016E-4	6.661948315336027E-4	0.0	5.701933804978125	5.075770145017925E-4
64-65	1.5861781703181016E-4	6.899875040883742E-4	0.0	6.241849026927278	5.234387962049736E-4
66-67	1.5861781703181016E-4	7.296419583463267E-4	0.0	6.812948511704885	5.234387962049736E-4
68-69	1.5861781703181016E-4	7.296419583463267E-4	0.0	7.448062181991104	5.234387962049736E-4
70-71	1.5861781703181016E-4	7.415382946237125E-4	0.0	8.157329681739695	5.432660233339498E-4
72-73	1.5861781703181016E-4	7.534346309010983E-4	0.0	8.829912845854253	5.630932504629261E-4
74-75	1.5861781703181016E-4	7.534346309010983E-4	0.0	9.49856625355185	5.710241413145166E-4
76-77	1.5861781703181016E-4	7.613655217526889E-4	0.0	10.219888707394858	5.789550321661072E-4
78-79	1.5861781703181016E-4	7.613655217526889E-4	0.0	10.942686306936263	5.789550321661072E-4
80-81	1.5861781703181016E-4	7.692964126042793E-4	0.0	11.72486645172895	6.027477047208787E-4
82-83	1.5861781703181016E-4	7.811927488816651E-4	0.0	12.562578694264475	6.146440409982644E-4
84-85	1.5861781703181016E-4	7.930890851590509E-4	0.0	13.37712877006793	6.22574931849855E-4
86-87	1.6654870788340067E-4	8.168817577138223E-4	0.0	14.201065055194242	6.305058227014453E-4
88	1.6654870788340067E-4	8.327435394170034E-4	0.0	14.841361562651976	6.344712681272406E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	6785	0.0	24.992882	1
GTATAAG	7150	0.0	23.191435	1
GTGATCG	5050	0.0	23.157932	8
ACGTTAA	1885	0.0	22.42451	8
TAGGCAT	10120	0.0	20.607008	5
TGATCGC	5825	0.0	19.996204	9
TAGTACT	7970	0.0	19.920574	4
CTAGGCA	11030	0.0	18.993406	4
CGTTAAC	2205	0.0	18.957157	9
CTGTGCG	8200	0.0	18.72948	9
ATAAGGT	7190	0.0	18.684834	3
GTACATA	3350	0.0	18.64944	1
CTAGTAC	9095	0.0	18.334867	3
GACGTTA	2415	0.0	18.086916	7
GCATCGT	8300	0.0	17.768196	8
TCTAACG	3945	0.0	17.741611	2
CATGGGG	31275	0.0	17.57244	4
TAAGGTG	7255	0.0	17.481144	4
TATTCGC	1480	0.0	17.453913	9
CTATTGA	6105	0.0	17.386597	9
>>END_MODULE