FastQCFastQC Report
Fri 27 May 2016
ERR765573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13459752
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1286890.9561023115433329No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1178800.8757962256659707No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG547940.4070951678753071No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG474120.3522501751889634No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG452070.33586800113404763No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC363510.27007184084818203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346510.2574415932774987No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC341060.2533924844974856No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC313580.23297606077734567No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA304810.22646033894235199No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT304480.22621516354833282No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC271190.20148216698197707No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT267660.19885953322171168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239710.17809392030402937No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG181590.13491333272708145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159890.11879119318097392No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC147380.10949681688042989No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG143220.10640612100430974No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA136990.10177750674752403No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT136050.1010791283524392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG54100.042.7431451
TACATGA55400.035.630512
GTACATA41100.032.59131
GTACAAA52100.025.710221
TACAAGA42000.024.5062792
CAACACA221150.023.886575
GTACTAG40650.022.4305441
ACATGAG62500.022.4083083
TACATAG34000.022.1168672
GTATAAG47050.020.9777221
TACAAGG70850.020.7628522
CTATTGA48300.020.3363769
CATGGGG558450.019.9283734
GGGTACC80100.019.655627
AACACAA168800.019.4059456
CATGGGT137850.019.1603764
GTCCTAC36700.019.0817781
TGGGTAC84600.018.9434246
TAGGCAT57150.018.9141145
TAGTACT49200.018.9136164
Produced by FastQC (version 0.11.5)