FastQCFastQC Report
Fri 27 May 2016
ERR765572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7020908
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA326080.46444135146052334No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT289740.4126816645368377No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG128460.1829677870725553No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG100370.14295871702064747No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT90650.1291143538698983No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC90240.12853038381930088No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG84040.11969961720051026No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC81100.11551212464256759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG23700.027.8606741
GTACATA18000.022.7332651
TACATGA27350.022.272172
GTACAAG30950.021.1841031
TAGGCAT38250.019.572355
CGTTAAC7000.019.2642271
TAGTACT32600.018.9706654
ATGGGTA41600.018.5551015
CTAACGC12700.018.30693
TACGGGC19200.018.1638764
CTAGGCA41650.017.9746064
GTATAAG27350.017.8518661
TCTAACG12050.017.7508562
ATACGGG19400.017.7369333
GTAGGAC29250.017.6459733
CGGGCGT19400.017.4972466
GGGTACC42150.017.3201087
CATGGGT56700.017.3040584
TACTAGG38300.017.1186262
CTAGTAC35550.017.0040473
Produced by FastQC (version 0.11.5)