FastQCFastQC Report
Fri 27 May 2016
ERR765572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7020908
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT315730.4496996684759293No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA314230.44756319268106065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200370.2853904366785607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137440.19575815549783587No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG130870.186400391516311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107710.1534132052435383No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT95500.13602229227330712No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC94080.13399976185416473No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG92980.1324330129379277No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC92660.13197723143502238No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG89780.12787519790887447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6600.040.8673862
TCACGCA9250.030.667684
ATCACGC10850.026.1452583
AGTACTT337650.022.1274612-13
GTACTAG24950.020.8878631
GTATCAA644750.020.6766341
TAACGCA4950.020.6685644
TAGTACT34450.020.3835854
CATGGGG128850.019.3091074
TACTTTT393550.019.1085514-15
CTAACGC10850.018.4302643
CTAGTAC37100.018.300873
GAGTACT328050.018.23842612-13
ATGGGTA37850.017.6925095
CATGGGT48050.017.614524
CAACGCA751850.017.5415465
ATCAACG758900.017.378593
CCGTCGT39750.017.3129489
AACGCAG774950.017.2506086
CGTTAAC9500.017.1431661
Produced by FastQC (version 0.11.5)