##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765572_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7020908 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35845349347976 33.0 33.0 33.0 33.0 33.0 2 32.30495742146172 33.0 33.0 33.0 33.0 33.0 3 32.36526771181163 33.0 33.0 33.0 33.0 33.0 4 36.22851360536273 37.0 37.0 37.0 33.0 37.0 5 36.16460121682267 37.0 37.0 37.0 33.0 37.0 6 36.17142697212383 37.0 37.0 37.0 33.0 37.0 7 36.06086007678779 37.0 37.0 37.0 33.0 37.0 8 36.077191155332045 37.0 37.0 37.0 33.0 37.0 9 36.10454815815846 37.0 37.0 37.0 33.0 37.0 10-11 36.05886062030723 37.0 37.0 37.0 33.0 37.0 12-13 36.030628516995236 37.0 37.0 37.0 33.0 37.0 14-15 37.505319611081646 38.5 37.0 40.0 33.0 40.0 16-17 37.499530972347166 37.0 37.0 40.0 33.0 40.0 18-19 37.54485374256436 40.0 37.0 40.0 33.0 40.0 20-21 37.52643005719489 40.0 37.0 40.0 33.0 40.0 22-23 37.49101690265704 40.0 37.0 40.0 33.0 40.0 24-25 37.468317060984134 40.0 37.0 40.0 33.0 40.0 26-27 37.3303505757375 37.0 37.0 40.0 33.0 40.0 28-29 37.210177800364285 37.0 37.0 40.0 33.0 40.0 30-31 37.03906367951268 37.0 37.0 40.0 33.0 40.0 32-33 36.89694808705654 37.0 37.0 40.0 33.0 40.0 34-35 36.77845999406345 37.0 37.0 40.0 33.0 40.0 36-37 36.61494296749082 37.0 37.0 40.0 33.0 40.0 38-39 36.477135079964015 37.0 37.0 40.0 33.0 40.0 40-41 36.289992049461404 37.0 37.0 40.0 33.0 40.0 42-43 36.103483908349176 37.0 37.0 40.0 33.0 40.0 44-45 35.79564295672297 37.0 37.0 40.0 30.0 40.0 46-47 35.52591787273099 37.0 37.0 40.0 27.0 40.0 48-49 35.23805809732872 37.0 35.0 40.0 27.0 40.0 50-51 35.0181970765035 37.0 33.0 40.0 27.0 40.0 52-53 34.85614517381512 37.0 33.0 40.0 27.0 40.0 54-55 34.65404553086296 37.0 33.0 40.0 27.0 40.0 56-57 34.44384921437512 37.0 33.0 40.0 27.0 40.0 58-59 34.236987794171355 37.0 33.0 37.0 27.0 40.0 60-61 34.06950845958956 37.0 33.0 37.0 27.0 40.0 62-63 33.84416410242094 37.0 33.0 37.0 27.0 40.0 64-65 33.62843018310453 37.0 33.0 37.0 27.0 40.0 66-67 33.40233535320503 37.0 33.0 37.0 24.5 40.0 68-69 33.19808292602609 37.0 33.0 37.0 22.0 40.0 70-71 33.013256903523015 37.0 33.0 37.0 22.0 37.0 72-73 32.80567214383097 37.0 33.0 37.0 22.0 37.0 74-75 32.57799689157015 37.0 33.0 37.0 22.0 37.0 76-77 32.409389996279685 35.0 33.0 37.0 22.0 37.0 78-79 32.252575664002435 33.0 33.0 37.0 22.0 37.0 80-81 32.08850158697422 33.0 33.0 37.0 22.0 37.0 82-83 32.003900777506274 33.0 33.0 37.0 22.0 37.0 84-85 31.879536877566263 33.0 33.0 37.0 22.0 37.0 86-87 31.76653760453776 33.0 33.0 37.0 22.0 37.0 88-89 31.655358822534065 33.0 33.0 37.0 22.0 37.0 90-91 31.55567606070326 33.0 33.0 37.0 22.0 37.0 92-93 31.477018143522177 33.0 33.0 37.0 22.0 37.0 94-95 31.37947598800611 33.0 33.0 37.0 22.0 37.0 96-97 31.327861439004757 33.0 33.0 37.0 22.0 37.0 98-99 30.523510349373616 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03961871143924611 1101 2 0.10700696655271003 1101 3 0.05270477354034142 1101 4 0.06844816240250395 1101 5 0.11056398887268415 1101 6 0.08942201515819193 1101 7 0.13040222411608937 1101 8 0.14354502982368444 1101 9 0.10627985562997111 1101 10-11 0.1299529096088179 1101 12-13 0.10016880962434982 1101 14-15 0.19454297680228905 1101 16-17 0.18210829301372655 1101 18-19 0.15570787434991473 1101 20-21 0.22751015129224328 1101 22-23 0.20402194575937216 1101 24-25 0.21927232909530403 1101 26-27 0.19574502452485376 1101 28-29 0.2238347814121724 1101 30-31 0.20590804950797548 1101 32-33 0.278105636875857 1101 34-35 0.3140992068133315 1101 36-37 0.30094502481220076 1101 38-39 0.2963044530801682 1101 40-41 0.23443852361405249 1101 42-43 0.3519324891128903 1101 44-45 0.3639616256734328 1101 46-47 0.3531505823627654 1101 48-49 0.3086793446660465 1101 50-51 0.3815289743720669 1101 52-53 0.39469945340108126 1101 54-55 0.29524746509999744 1101 56-57 0.3369856761233265 1101 58-59 0.37971608904398124 1101 60-61 0.3272467792729543 1101 62-63 0.3420399391417064 1101 64-65 0.3341495371526335 1101 66-67 0.41806141064106583 1101 68-69 0.4243325439675729 1101 70-71 0.3169231280476197 1101 72-73 0.31257133171216367 1101 74-75 0.40167539183998 1101 76-77 0.46260767953810955 1101 78-79 0.3634388710819323 1101 80-81 0.38958950237392287 1101 82-83 0.3943763788383734 1101 84-85 0.3860515051511264 1101 86-87 0.3719434243775197 1101 88-89 0.3410486219621127 1101 90-91 0.4503586747943622 1101 92-93 0.37707269275845334 1101 94-95 0.4827942361803892 1101 96-97 0.514405816964846 1101 98-99 0.5446574691576203 1102 1 0.026692146575619802 1102 2 0.07229674948260367 1102 3 0.08272977719447283 1102 4 0.05110574794000655 1102 5 0.08941610424815138 1102 6 0.08915951201035455 1102 7 0.06152954287554735 1102 8 0.11575469657272919 1102 9 0.07831609786750704 1102 10-11 0.08890226011255464 1102 12-13 0.07720653479371009 1102 14-15 0.1461284994840284 1102 16-17 0.09397672254709732 1102 18-19 0.10859626380817389 1102 20-21 0.1678452586650181 1102 22-23 0.1571233094696396 1102 24-25 0.18028868551785848 1102 26-27 0.1570931695172888 1102 28-29 0.1190033697021633 1102 30-31 0.19117905474938368 1102 32-33 0.21965541290251878 1102 34-35 0.22290585172740407 1102 36-37 0.19655981342140905 1102 38-39 0.22776851329908254 1102 40-41 0.23668234769093743 1102 42-43 0.21039561628078474 1102 44-45 0.2965754350046481 1102 46-47 0.33057802656607294 1102 48-49 0.2650394345606202 1102 50-51 0.2538362717467848 1102 52-53 0.23590352140374193 1102 54-55 0.048255960296202716 1102 56-57 0.16729716591728305 1102 58-59 0.21489625095284026 1102 60-61 0.11181110622882784 1102 62-63 0.1784360904750102 1102 64-65 0.11329334837401461 1102 66-67 0.18512646328697713 1102 68-69 0.2276599392143197 1102 70-71 0.020556670571437508 1102 72-73 0.14346643699518324 1102 74-75 0.23587510174006354 1102 76-77 0.2291197145461581 1102 78-79 0.19583376636243344 1102 80-81 0.19042091643196812 1102 82-83 0.22432129977117654 1102 84-85 0.1860454222689576 1102 86-87 0.2182620112586875 1102 88-89 0.31975413180596135 1102 90-91 0.16895544706592958 1102 92-93 0.23850364639588406 1102 94-95 0.28668134463795525 1102 96-97 0.40867125501160473 1102 98-99 0.32241619563542656 1103 1 0.017977537370249763 1103 2 0.08888282319147578 1103 3 0.10993086785079953 1103 4 0.07077873018997138 1103 5 0.05994925031153997 1103 6 0.05651549449679294 1103 7 0.06740901890189122 1103 8 0.12995458335085175 1103 9 0.08051908749838788 1103 10-11 0.09405580832859783 1103 12-13 0.08052520780462658 1103 14-15 0.14813652175578085 1103 16-17 0.14495000075044828 1103 18-19 0.15417091858893173 1103 20-21 0.05989962666478732 1103 22-23 0.10343691615976525 1103 24-25 0.1391293615527971 1103 26-27 0.2141820415263993 1103 28-29 0.0673227593072454 1103 30-31 0.17843258218418612 1103 32-33 0.11938119022804727 1103 34-35 0.1678449053931459 1103 36-37 0.13008470431994823 1103 38-39 0.031048956010963025 1103 40-41 0.05435923896209971 1103 42-43 0.09500206276195655 1103 44-45 0.12608404846658772 1103 46-47 0.1744283228777661 1103 48-49 0.1446936778730077 1103 50-51 0.1936090755647939 1103 52-53 0.1412399086225662 1103 54-55 0.07450113224297894 1103 56-57 0.15122596512043884 1103 58-59 0.2364116505615783 1103 60-61 0.1755454217739043 1103 62-63 0.2372271602784437 1103 64-65 0.1640194374180055 1103 66-67 0.23686058535162857 1103 68-69 0.21950955834452657 1103 70-71 0.07709387255783895 1103 72-73 0.10256338502060913 1103 74-75 0.1637237427192204 1103 76-77 0.14242968093789443 1103 78-79 0.14324505200404047 1103 80-81 0.17553206018792622 1103 82-83 0.13298592653310237 1103 84-85 0.13820083004688044 1103 86-87 0.07193817518676582 1103 88-89 0.17761054783187546 1103 90-91 0.13532134721569733 1103 92-93 0.17656038632236104 1103 94-95 0.04970392093732201 1103 96-97 0.08392263624687146 1103 98-99 0.1156490923240483 1104 1 -0.06448163369305604 1104 2 -0.013072702543475145 1104 3 -0.06205110967461991 1104 4 -0.04172391858674018 1104 5 -0.10135799519453315 1104 6 0.022460986359789104 1104 7 0.060813295079810814 1104 8 0.06289216216207194 1104 9 0.02168516984521318 1104 10-11 -0.015010504628186538 1104 12-13 -0.03768084183947451 1104 14-15 0.04541268438437385 1104 16-17 6.273590611343138E-4 1104 18-19 -0.022330011406012318 1104 20-21 0.016543361251066813 1104 22-23 0.04364133966183914 1104 24-25 -0.010642542346531059 1104 26-27 -0.00189146405087115 1104 28-29 -0.06955197648964884 1104 30-31 -0.1018417258510027 1104 32-33 -0.059481567161995486 1104 34-35 -0.03357954286082787 1104 36-37 0.07033213334262456 1104 38-39 -0.028899152249799442 1104 40-41 -0.019779081146836575 1104 42-43 0.00508722835321862 1104 44-45 -0.026805398159439164 1104 46-47 -0.09458862569549353 1104 48-49 -0.1154143238610601 1104 50-51 -0.13268552416861468 1104 52-53 -0.04406740793728403 1104 54-55 -0.12835349803456353 1104 56-57 -0.09366354176638225 1104 58-59 -0.07842254680961247 1104 60-61 -0.07965923107895634 1104 62-63 -0.08765565496195649 1104 64-65 -0.007267058587693498 1104 66-67 -0.0010684760010590821 1104 68-69 -0.06147918188108292 1104 70-71 -0.12025493131036313 1104 72-73 -0.026524518389429375 1104 74-75 -0.06358905757291211 1104 76-77 -0.036245377569045445 1104 78-79 -0.014796647566221566 1104 80-81 0.03286067219295319 1104 82-83 0.02424007058697697 1104 84-85 0.03942272108456635 1104 86-87 0.08872981854360162 1104 88-89 0.05075880557538781 1104 90-91 0.0011429806609761783 1104 92-93 0.0188971650141454 1104 94-95 -0.010767089123532969 1104 96-97 0.01226987153559378 1104 98-99 0.08200459593339815 1105 1 0.015206592118566675 1105 2 0.09158214944955745 1105 3 0.05588140584659129 1105 4 0.03332035112655518 1105 5 0.07735409236116197 1105 6 0.04922269770639076 1105 7 0.03058790272035594 1105 8 0.11019124198302421 1105 9 -0.030686882530687853 1105 10-11 0.004045021281108063 1105 12-13 -0.001456838872442745 1105 14-15 -0.0031651926189582014 1105 16-17 0.02394162842620773 1105 18-19 0.07645596533426158 1105 20-21 0.06100181348157463 1105 22-23 0.016348198054856766 1105 24-25 0.013790044361961407 1105 26-27 -0.00869067393885814 1105 28-29 -0.01975592006056104 1105 30-31 0.01232731898022621 1105 32-33 0.012831728223837047 1105 34-35 0.06833095706243597 1105 36-37 0.0969767043129437 1105 38-39 0.06495240996667917 1105 40-41 -0.05113200711911503 1105 42-43 -0.02701372790018297 1105 44-45 0.05159124975070739 1105 46-47 0.01440884930318731 1105 48-49 0.10733319181258594 1105 50-51 0.09306090100454867 1105 52-53 0.07460026427568067 1105 54-55 0.07307765770215724 1105 56-57 0.10134973828523641 1105 58-59 0.12539913408561887 1105 60-61 0.029297292549884446 1105 62-63 0.05051071127819995 1105 64-65 0.1290784620413561 1105 66-67 0.12052342031899599 1105 68-69 0.08781376883767678 1105 70-71 0.028800099025446002 1105 72-73 0.03722449230993874 1105 74-75 0.06950218682874976 1105 76-77 0.03848713104615342 1105 78-79 0.08339117383071937 1105 80-81 0.007489640178370394 1105 82-83 0.04009151444250847 1105 84-85 -0.04324602591168869 1105 86-87 0.09223595896269643 1105 88-89 0.14036142436153298 1105 90-91 0.06385484263918784 1105 92-93 0.0685580775003487 1105 94-95 0.06639695351114128 1105 96-97 0.049301001236798925 1105 98-99 0.19248608931505373 1106 1 -0.023860521463575424 1106 2 0.08667042951740456 1106 3 0.019821401312817954 1106 4 0.009210391360923609 1106 5 0.04404183465752709 1106 6 0.11343726252650299 1106 7 0.009223806891156983 1106 8 0.041847423104314885 1106 9 0.003983870202759476 1106 10-11 0.028937613057969713 1106 12-13 0.024073478779143898 1106 14-15 0.03965604466014838 1106 16-17 0.04637482448637087 1106 18-19 0.009928299674569985 1106 20-21 0.021397061327085964 1106 22-23 0.038239776758224764 1106 24-25 0.0974398124665612 1106 26-27 0.07037682525309918 1106 28-29 0.03186029242444732 1106 30-31 0.05620649544614764 1106 32-33 0.0772860041565906 1106 34-35 0.19122097125153203 1106 36-37 0.12050004471019804 1106 38-39 0.009812928481018446 1106 40-41 0.06054992040104423 1106 42-43 0.026687171674005583 1106 44-45 0.08913254209959831 1106 46-47 0.1500535513842891 1106 48-49 0.1107560880948526 1106 50-51 0.08330697910948714 1106 52-53 0.043470327237862705 1106 54-55 -0.001893840526847157 1106 56-57 0.16395780285871098 1106 58-59 0.1530408912528074 1106 60-61 -0.0040223348508448 1106 62-63 0.05077037595221867 1106 64-65 0.07969585138605595 1106 66-67 0.09311883107996977 1106 68-69 0.1020681773074017 1106 70-71 0.05468930814542716 1106 72-73 0.052617429521120584 1106 74-75 0.06875937674143273 1106 76-77 0.16178796963845343 1106 78-79 0.03818045071867715 1106 80-81 0.08346069510944432 1106 82-83 -0.012574973596336747 1106 84-85 0.14116742087680834 1106 86-87 0.14793448096185458 1106 88-89 0.18370716255644481 1106 90-91 0.029412086181974217 1106 92-93 0.06867469763185241 1106 94-95 0.07258513479863637 1106 96-97 0.037022285018263545 1106 98-99 0.1050211823855065 1107 1 -0.006061322920452028 1107 2 0.06799389533111366 1107 3 0.06538470212804981 1107 4 -0.060843543382901544 1107 5 0.04797576925339797 1107 6 0.015241815027998484 1107 7 0.055934126274031826 1107 8 0.03605469648274351 1107 9 0.05214387129267806 1107 10-11 -0.012512039861462654 1107 12-13 -0.05235820272660874 1107 14-15 0.05191515289421744 1107 16-17 -0.019194512352562754 1107 18-19 -0.034392280533523945 1107 20-21 0.01763074756461691 1107 22-23 0.052308064431329626 1107 24-25 0.049148953315139465 1107 26-27 0.05935517803086299 1107 28-29 0.012338155905041504 1107 30-31 -0.009520959807005624 1107 32-33 0.04980905402962321 1107 34-35 0.006034227667747416 1107 36-37 0.06498654778528845 1107 38-39 -0.09647310298955603 1107 40-41 -0.12410790120797799 1107 42-43 -0.01838293642996547 1107 44-45 -0.017426436006147128 1107 46-47 0.08301883355308348 1107 48-49 0.01769820658753929 1107 50-51 -0.0274334400209284 1107 52-53 -0.08832400917972194 1107 54-55 -0.1081097817183263 1107 56-57 -0.0668294729475889 1107 58-59 -0.054498588390863745 1107 60-61 -0.062095915727084616 1107 62-63 -0.09088642552126913 1107 64-65 -0.009813309623886823 1107 66-67 0.018025224928543082 1107 68-69 -0.13306187980602857 1107 70-71 -0.058980123955365116 1107 72-73 0.016008784266169584 1107 74-75 -0.02189131310741743 1107 76-77 -0.08113117989611851 1107 78-79 -0.050394812031363756 1107 80-81 -0.08692482761892961 1107 82-83 -0.18013534467053205 1107 84-85 -0.021467098607818258 1107 86-87 -0.034194914689472 1107 88-89 -0.009518962399855724 1107 90-91 -0.0011638785838776755 1107 92-93 0.06740462880287268 1107 94-95 -0.14162597201720928 1107 96-97 -0.011861807945408032 1107 98-99 -0.022308008679697622 1108 1 -0.06581629574594672 1108 2 0.007534979070861425 1108 3 -0.028679853632610275 1108 4 -0.037134929650420645 1108 5 -0.052877048783699365 1108 6 -0.008738576228232375 1108 7 -0.10484830805894774 1108 8 -0.09599820122987524 1108 9 -0.05806700568984979 1108 10-11 -0.08862602972357791 1108 12-13 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-0.15606183930088946 2215 72-73 -0.1448011224313177 2215 74-75 -0.2414943774383147 2215 76-77 -0.20255407025261007 2215 78-79 -0.13856039987374658 2215 80-81 -0.1396375709480111 2215 82-83 -0.17610719684201825 2215 84-85 -0.13275749577875828 2215 86-87 -0.06822064486008017 2215 88-89 -0.039669144052087546 2215 90-91 -0.13868396823643891 2215 92-93 -0.05528531755675914 2215 94-95 -0.08937317885932572 2215 96-97 -0.12101021273251078 2215 98-99 -0.14349322794159747 2216 1 -0.0688543634815062 2216 2 -0.0959942391102544 2216 3 -0.11358367169543726 2216 4 -0.19204637765215438 2216 5 -0.09070497900202668 2216 6 -0.22782683483115562 2216 7 -0.11223287101485369 2216 8 -0.16184901329409485 2216 9 -0.09457263050509823 2216 10-11 -0.07785534816891726 2216 12-13 -0.046226176858823464 2216 14-15 -0.2217239098911037 2216 16-17 -0.25321178795168464 2216 18-19 -0.08659841836569626 2216 20-21 -0.14875362669498315 2216 22-23 -0.2560877626338822 2216 24-25 -0.26474657090068376 2216 26-27 -0.25142538531186887 2216 28-29 -0.19725594494781973 2216 30-31 -0.1934224067029362 2216 32-33 -0.14499305093307413 2216 34-35 -0.24222199616234974 2216 36-37 -0.2257011395552908 2216 38-39 -0.21932023215323682 2216 40-41 -0.2148798534782017 2216 42-43 -0.2468575932259327 2216 44-45 -0.2917694370495454 2216 46-47 -0.17650849223389997 2216 48-49 -0.12276307314431278 2216 50-51 -0.13228457949817596 2216 52-53 -0.16531725195508784 2216 54-55 -0.11549427799015177 2216 56-57 -0.18956107743021988 2216 58-59 -0.290199496810537 2216 60-61 -0.235728708335742 2216 62-63 -0.16620225784583198 2216 64-65 -0.2517930634520056 2216 66-67 -0.20834867244401067 2216 68-69 -0.12745940524203547 2216 70-71 -0.23803713106602942 2216 72-73 -0.19487989443161524 2216 74-75 -0.17239961722213337 2216 76-77 -0.16950352345405406 2216 78-79 -0.030783893300785792 2216 80-81 -0.2479498367198012 2216 82-83 -0.23203157412810071 2216 84-85 -0.23247853278367003 2216 86-87 -0.2905549459629704 2216 88-89 -0.22815367348536952 2216 90-91 -0.30428039094100257 2216 92-93 -0.08637025135750065 2216 94-95 -0.1727999921579375 2216 96-97 -0.19762548013136438 2216 98-99 -0.23234428248087724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 23.0 10 67.0 11 153.0 12 310.0 13 652.0 14 1754.0 15 4752.0 16 10463.0 17 19494.0 18 30942.0 19 41546.0 20 51394.0 21 63572.0 22 74132.0 23 67469.0 24 55198.0 25 52971.0 26 62979.0 27 80404.0 28 105760.0 29 137245.0 30 179278.0 31 233004.0 32 303891.0 33 398100.0 34 529392.0 35 762338.0 36 1280076.0 37 1854953.0 38 617697.0 39 895.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33048071489801 18.665045828348376 12.86907621479148 25.13539724196214 2 16.151728522863422 21.419181108768267 36.66565635100189 25.763434017366414 3 18.21707391693496 26.342077121648654 29.440095212755956 26.000753748660433 4 13.101724164452802 17.29348967398519 36.10503085925638 33.49975530230563 5 14.038141185201328 37.170911737705104 33.1492152618868 15.641731815206764 6 31.713832455858988 36.70914075501345 17.161412740346403 14.415614048781155 7 26.954277139082294 31.79336632811596 22.60666284190022 18.645693690901517 8 27.44223396745834 31.816981507235248 20.433610581423373 20.307173943883043 9 27.476730929959487 13.713553859415335 19.947391420027152 38.862323790598026 10-11 25.770071335502475 24.743508959240028 26.862266248183282 22.624153457074215 12-13 27.03714391357927 22.675407511393114 26.984031125318836 23.303417449708785 14-15 24.548136183716693 23.31116352671558 25.41440375113413 26.726296538433598 16-17 23.55147793374508 25.545069503007262 26.484476993440232 24.418975569807433 18-19 24.003066010089093 24.059946579125523 29.177930139807838 22.75905727097755 20-21 24.980939034393487 23.67401839053807 28.251384830960962 23.093657744107478 22-23 23.96415107561586 23.40559084380539 28.13294947035341 24.497308610225343 24-25 23.715109213794 24.128987304775965 28.03883059000346 24.11707289142658 26-27 24.20689745542884 23.77770083299767 27.945195977500347 24.070205734073145 28-29 23.59022508199794 23.603428502410228 28.870069227513024 23.936277188078805 30-31 24.29063836636096 23.64691655901175 28.37034193670603 23.69210313792126 32-33 23.56606866234396 23.74939965030164 27.938921860249415 24.745609827104982 34-35 24.102238627824207 23.859029344922337 28.45091404131773 23.58781798593572 36-37 24.42467555478579 23.80615869058532 27.595846577109402 24.173319177519488 38-39 24.549958495396893 23.29403832096931 27.70848870260086 24.447514481032936 40-41 24.165264663772835 23.80237000684242 27.894547257989995 24.137818071394754 42-43 25.048013732696685 23.60820708660475 27.309010458476312 24.034768722222253 44-45 24.577704194386254 23.930907512247703 26.723979291567417 24.767409001798626 46-47 24.156262979090453 24.021387262160392 27.1183228721983 24.704026886550857 48-49 25.263527167711068 24.46148703273138 25.977451919324395 24.297533880233154 50-51 24.076657890973646 24.96156480045031 25.769188258840593 25.192589049735446 52-53 24.181395056024094 24.702545596666415 25.4241474179693 25.691911929340193 54-55 24.446104407008324 24.741721441158322 25.442058206716283 25.37011594511707 56-57 24.60956617007373 24.080033522729536 25.23296132067248 26.07743898652425 58-59 24.042598769646144 24.19846894425464 26.05943932885025 25.69949295724897 60-61 24.258557440148767 24.40758374842684 25.73631501794355 25.597543793480842 62-63 23.627107775803356 24.547408967615013 25.667235633909456 26.158247622672164 64-65 23.666397565670994 24.207424452791575 25.866725500462334 26.259452481075098 66-67 24.503604092234223 24.28645269244377 25.677811189094058 25.532132026227945 68-69 24.525168254590433 23.716319883411092 25.719878397495027 26.03863346450345 70-71 23.582847118919663 23.993470645107443 25.97090006022013 26.452782175752766 72-73 24.415709478033325 24.06225804411623 25.789214158624496 25.73281831922595 74-75 24.829761975129987 23.7332580343987 25.726555194807503 25.710424795663805 76-77 24.104219379804718 24.17372612334755 25.97278996151705 25.749264535330685 78-79 24.286110652955617 24.258372067650527 25.80963197627201 25.645885303121847 80-81 23.709568252302482 24.131999488385116 26.1423383249968 26.0160939343156 82-83 23.76784625064495 24.461830610928857 25.38540779806599 26.384915340360205 84-85 23.77285699615191 24.53340257440626 25.117118492679197 26.576621936762635 86-87 23.70546639017589 23.918432087059557 25.463060094299138 26.913041428465416 88-89 23.69442234965627 23.715359876562598 25.735895317493025 26.854322456288106 90-91 24.107200847761806 23.94083186627241 25.31981215922881 26.632155126736972 92-93 23.583402176390155 24.157482053327257 25.262477210574293 26.9966385597083 94-95 22.90472186067923 24.295204814698653 25.903895463131587 26.89617786149053 96-97 23.84537872674511 23.981646132192772 25.385248836147166 26.787726304914948 98-99 23.480541896075078 23.914412843485913 25.460240555777553 27.144804704661457 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 99.0 1 109.0 2 341.0 3 578.0 4 403.0 5 332.5 6 699.5 7 1894.0 8 3335.0 9 3169.5 10 1904.0 11 1223.5 12 1206.0 13 1359.0 14 1652.5 15 2106.5 16 2619.0 17 3234.5 18 4129.0 19 5214.5 20 6558.5 21 8259.0 22 10403.5 23 13250.0 24 16456.5 25 20670.0 26 25925.0 27 30563.0 28 36249.5 29 43827.5 30 50422.5 31 57114.5 32 65032.5 33 72892.0 34 79110.0 35 84949.5 36 90436.0 37 97597.5 38 109585.5 39 115188.0 40 118360.5 41 125572.0 42 139232.0 43 162541.5 44 185667.0 45 210799.0 46 251109.0 47 288824.0 48 313503.5 49 345683.0 50 387177.5 51 368494.0 52 320949.0 53 311281.0 54 297660.0 55 287492.0 56 266798.5 57 248526.0 58 243186.0 59 217757.5 60 180286.5 61 137883.5 62 114208.0 63 92924.5 64 67864.5 65 53361.5 66 38387.5 67 28490.0 68 22461.5 69 19547.0 70 18585.5 71 17664.5 72 19013.0 73 16377.5 74 10042.0 75 7632.5 76 6019.5 77 3898.5 78 1931.5 79 1124.5 80 779.5 81 554.5 82 417.0 83 315.0 84 209.0 85 135.0 86 68.0 87 31.5 88 13.0 89 8.0 90 5.0 91 3.5 92 2.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056445690500430995 2 0.0 3 0.0 4 0.0 5 0.005882430021871815 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013958308526475494 16-17 0.004450991239309787 18-19 8.118608020501052E-4 20-21 2.1364757948686978E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.563770953842437E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.424317196579132E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.272951589737396E-5 76-77 9.258061777764357E-5 78-79 2.848634393158264E-5 80-81 2.848634393158264E-5 82-83 7.12158598289566E-6 84-85 3.845656430763656E-4 86-87 8.403471459816878E-4 88-89 3.0622819726451334E-4 90-91 9.685356936738097E-4 92-93 0.0015382625723054624 94-95 0.0010539947254685576 96-97 0.0021863268967489678 98-99 5.910916365803397E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7020908.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.849965007749326 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0705851479184 25.116573704929746 2 15.317426947886167 10.676235862851792 3 5.309932237926126 5.551528580557368 4 2.34404679000518 3.2675979443283065 5 1.2617305225003432 2.198563227917312 6 0.7636768227908503 1.596846633089419 7 0.5010080690922636 1.222207957352581 8 0.35491068771288314 0.9894900038136182 9 0.26272835849311654 0.824046669002568 >10 1.445844227630665 9.493159910213718 >50 0.1575085111057252 3.8833088953705053 >100 0.16401868067893233 12.182919380498976 >500 0.0265896081192515 6.471762607992266 >1k 0.018723316352235463 12.446262515234682 >5k 0.001024251441581867 2.3570634534985038 >10k+ 2.458203459796481E-4 1.722432653348873 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 31573 0.4496996684759293 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 31423 0.44756319268106065 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20037 0.2853904366785607 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13744 0.19575815549783587 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 13087 0.186400391516311 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10771 0.1534132052435383 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 9550 0.13602229227330712 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 9408 0.13399976185416473 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 9298 0.1324330129379277 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 9266 0.13197723143502238 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 8978 0.12787519790887447 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.4243171965791319E-5 0.0 2 0.0 0.0 0.0 1.4243171965791319E-5 0.0 3 0.0 0.0 0.0 1.4243171965791319E-5 0.0 4 0.0 0.0 0.0 1.4243171965791319E-5 0.0 5 0.0 0.0 0.0 2.8486343931582637E-5 0.0 6 0.0 0.0 0.0 2.8486343931582637E-5 0.0 7 0.0 0.0 0.0 4.272951589737396E-5 0.0 8 0.0 0.0 0.0 4.272951589737396E-5 0.0 9 0.0 0.0 0.0 4.272951589737396E-5 0.0 10-11 7.121585982895659E-6 0.0 0.0 6.409427384606094E-5 0.0 12-13 1.4243171965791319E-5 0.0 0.0 8.545903179474792E-5 0.0 14-15 1.4243171965791319E-5 0.0 0.0 1.0682378974343489E-4 0.0 16-17 1.4243171965791319E-5 0.0 0.0 1.3531013367501754E-4 0.0 18-19 1.4243171965791319E-5 0.0 0.0 2.2076916546976544E-4 0.0 20-21 2.8486343931582637E-5 0.0 0.0 5.483621206829658E-4 0.0 22-23 2.8486343931582637E-5 0.0 0.0 0.002157840552817385 0.0 24-25 3.56079299144783E-5 0.0 0.0 0.0056830256143507365 0.0 26-27 4.272951589737396E-5 0.0 0.0 0.010433123464942141 0.0 28-29 4.9851101880269616E-5 0.0 0.0 0.026129098971244175 0.0 30-31 5.6972687863165274E-5 0.0 0.0 0.07105206335135 0.0 32-33 6.409427384606094E-5 0.0 0.0 0.14248157075979345 0.0 34-35 7.12158598289566E-5 0.0 0.0 0.22931506864924023 0.0 36-37 7.12158598289566E-5 0.0 0.0 0.3617836895170824 0.0 38-39 7.12158598289566E-5 0.0 0.0 0.5633316944190125 0.0 40-41 7.12158598289566E-5 0.0 0.0 0.8168886417540295 0.0 42-43 7.12158598289566E-5 0.0 0.0 1.0351794953017475 0.0 44-45 7.12158598289566E-5 0.0 0.0 1.2794570161010514 0.0 46-47 7.12158598289566E-5 0.0 0.0 1.5480974825478415 0.0 48-49 7.12158598289566E-5 0.0 0.0 1.826387698001455 0.0 50-51 7.12158598289566E-5 0.0 0.0 2.1464602584167176 0.0 52-53 7.12158598289566E-5 0.0 0.0 2.465393365074717 0.0 54-55 7.833744581185226E-5 0.0 0.0 2.771386550001795 0.0 56-57 8.545903179474792E-5 7.121585982895659E-6 0.0 3.1115704692327544 0.0 58-59 9.258061777764357E-5 1.4243171965791319E-5 0.0 3.4823914513621315 0.0 60-61 9.970220376053923E-5 1.4243171965791319E-5 0.0 3.854757817649797 0.0 62-63 9.970220376053923E-5 1.4243171965791319E-5 0.0 4.234160310888563 0.0 64-65 1.1394537572633055E-4 1.4243171965791319E-5 0.0 4.628454040417564 0.0 66-67 1.1394537572633055E-4 1.4243171965791319E-5 0.0 5.055015676035065 0.0 68-69 1.1394537572633055E-4 1.4243171965791319E-5 0.0 5.519442784323623 0.0 70-71 1.1394537572633055E-4 1.4243171965791319E-5 0.0 6.061395477621983 0.0 72-73 1.1394537572633055E-4 1.4243171965791319E-5 0.0 6.56775448417783 0.0 74-75 1.1394537572633055E-4 1.4243171965791319E-5 0.0 7.055191436777124 0.0 76-77 1.1394537572633055E-4 1.4243171965791319E-5 0.0 7.581968030345932 0.0 78-79 1.1394537572633055E-4 1.4243171965791319E-5 0.0 8.127125437336595 0.0 80-81 1.1394537572633055E-4 1.4243171965791319E-5 0.0 8.716208786669759 0.0 82-83 1.1394537572633055E-4 1.4243171965791319E-5 0.0 9.341868601611074 0.0 84-85 1.2818854769212188E-4 1.4243171965791319E-5 0.0 9.952038967039591 0.0 86-87 1.2818854769212188E-4 1.4243171965791319E-5 0.0 10.571695284997325 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 660 0.0 40.867386 2 TCACGCA 925 0.0 30.66768 4 ATCACGC 1085 0.0 26.145258 3 AGTACTT 33765 0.0 22.12746 12-13 GTACTAG 2495 0.0 20.887863 1 GTATCAA 64475 0.0 20.676634 1 TAACGCA 495 0.0 20.668564 4 TAGTACT 3445 0.0 20.383585 4 CATGGGG 12885 0.0 19.309107 4 TACTTTT 39355 0.0 19.10855 14-15 CTAACGC 1085 0.0 18.430264 3 CTAGTAC 3710 0.0 18.30087 3 GAGTACT 32805 0.0 18.238426 12-13 ATGGGTA 3785 0.0 17.692509 5 CATGGGT 4805 0.0 17.61452 4 CAACGCA 75185 0.0 17.541546 5 ATCAACG 75890 0.0 17.37859 3 CCGTCGT 3975 0.0 17.312948 9 AACGCAG 77495 0.0 17.250608 6 CGTTAAC 950 0.0 17.143166 1 >>END_MODULE